pycom05g07040

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000397
Physical Location & Seq
Reverse (-)
9982821 .. 9983911
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07040.1

Sequence Viewer

Length: 960 bp
ATGGTAATGCCCATGAGTAACGATATAACCTATGATTTCTCTCCTCTCTTCAAAATCTACAAAGATGGTCGAATTGAGCGGCTCACCGGCACAACCAAGCTTCCTCCATCCCTTGATCCCCAAACTGGAGTCGAATCCAAAGATGCTGTGGTCTCACAAGAACCAGCATTGTCCGGAAGGCTTTACCTCCCCACCAAATCCACCACCAACTCACCCGATAAAAAACTCCCTCTTCTTGTTTACATCCACGGAGGTGGTTTCGTGGTTGAAACTGCATTTTCTCCAATTTATCACACCCACCTTAACGTACTAGTTGCTCAAGCCAATGTTGTTGCTGTCTCAGTTGACTACAGGCTAGCCCCAGAGCACTCTCTGCCAGCTGCTTACAACGATGCATGGGCTGCTATCAAATGGGTTGCTTCCCATTCTGAGGGGAAGGGCTCCGAAGAGTGGCTAAATCGCCACGCAGATTTTCAGCGCGTGTTTTTTGCAGGTGACAGCGCTGGAGCTAACATAGCTCACCACATGGGATTGAGAGTAGGATCCGAGGGGTTGAGTGGTGTTAAGCTGAATGGCATTGTGTTGGTGCATCCATATTTTTGGGGAACAGAGCCGATTGGGGAAGAATCAGCTCTGCCCCCTATTGCAAGAGAGTATATGGCTGGTTTGTGGCGTGTGGCGTACCCCTCAACAAGTGGATCCGATGACCCGTTTTTGAATCCTGGTAAGGATCCAAAATTGGGGGAATTGGGCTGTGAGAAAGTGCTGGTTTGTGTTGGAGAGAAAGATGTCTTGAAAGAAAGGGGATGCAATTATAGTGACGTACTAAAAAAGAGTGGATGGAAAGGGGCTGTGGAGGTCATCGAAGCAAAGGGGGAGAACCATGTGTTCCATTTGTTCAATCCCACTTGCGACAATGCTGTGGCAATGTTCAAAAAAATTGTTTCTTTCATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

320

Amino Acids

34.84

Weight (kDa)

6.13

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 53 - 178 5.3e-09 Carboxylesterase family
BD-FAE PF20434 72 - 182 1.3e-14 BD-FAE
Abhydrolase_3 PF07859 79 - 298 3.7e-55 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 482
Acc36I ACCTGC 1 cut(s) 482
AccBSI CCGCTC 1 cut(s) 79
AccII CGCG 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 173
AciI CCGC 1 cut(s) 79
AclWI GGATC 7 cut(s) 110, 537, 550, 693, 706, 725, 738
AfaI GTAC 3 cut(s) 309, 683, 825
AfeI AGCGCT 1 cut(s) 502
AfiI CCNNNNNNNGG 4 cut(s) 125, 430, 450, 740
AgsI TTSAA 6 cut(s) 52, 269, 718, 796, 901, 934
AhlI ACTAGT 1 cut(s) 310
AjnI CCWGG 1 cut(s) 721
AleI CACNNNNGTG 1 cut(s) 252
AluBI AGCT 6 cut(s) 100, 380, 509, 518, 568, 632
AluI AGCT 6 cut(s) 100, 380, 509, 518, 568, 632
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 2 cut(s) 157, 343
AlwI GGATC 7 cut(s) 110, 537, 550, 693, 706, 725, 738
Aor13HI TCCGGA 1 cut(s) 173
Aor51HI AGCGCT 1 cut(s) 502
ApeKI GCWGC 2 cut(s) 380, 401
AseI ATTAAT 1 cut(s) 954
AspLEI GCGC 2 cut(s) 480, 503
AsuHPI GGTGA 4 cut(s) 76, 204, 506, 512
AsuNHI GCTAGC 1 cut(s) 355
BamHI GGATCC 3 cut(s) 542, 698, 730
BanII GRGCYC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 2 cut(s) 367, 388
BccI CCATC 3 cut(s) 59, 115, 834
BciT130I CCWGG 1 cut(s) 723
BcoDI GTCTC 2 cut(s) 157, 343
BcuI ACTAGT 1 cut(s) 310
BfaI CTAG 2 cut(s) 311, 356
BfmI CTRYAG 1 cut(s) 349
BfoI RGCGCY 1 cut(s) 504
BfuAI ACCTGC 1 cut(s) 482
BisI GCNGC 3 cut(s) 80, 381, 402
BlsI GCNGC 3 cut(s) 81, 382, 403
Bme1390I CCNGG 1 cut(s) 723
BmiI GGNNCC 4 cut(s) 442, 544, 700, 732
BmrFI CCNGG 1 cut(s) 723
BmsI GCATC 4 cut(s) 133, 382, 598, 797
BmtI GCTAGC 1 cut(s) 359
BpmI CTGGAG 2 cut(s) 147, 525
BpuEI CTTGAG 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 247, 546
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 430, 450, 740
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 130
Bse3DI GCAATG 1 cut(s) 933
BseAI TCCGGA 1 cut(s) 173
BseBI CCWGG 1 cut(s) 723
BseDI CCNNGG 2 cut(s) 247, 546
BseGI GGATG 5 cut(s) 107, 243, 589, 812, 845
BseLI CCNNNNNNNGG 4 cut(s) 125, 430, 450, 740
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 2 cut(s) 354, 420
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 367, 388
Bsh1236I CGCG 1 cut(s) 480
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 2 cut(s) 87, 174
BslI CCNNNNNNNGG 4 cut(s) 125, 430, 450, 740
BsmAI GTCTC 2 cut(s) 157, 343
Bso31I GGTCTC 1 cut(s) 157
Bsp1286I GDGCHC 2 cut(s) 369, 443
Bsp13I TCCGGA 1 cut(s) 173
Bsp143I GATC 4 cut(s) 115, 542, 698, 730
BspACI CCGC 1 cut(s) 79
BspCNI CTCAG 2 cut(s) 353, 421
BspEI TCCGGA 1 cut(s) 173
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 4 cut(s) 442, 544, 700, 732
BspMI ACCTGC 1 cut(s) 482
BspOI GCTAGC 1 cut(s) 359
BspPI GGATC 7 cut(s) 110, 537, 550, 693, 706, 725, 738
BspTNI GGTCTC 1 cut(s) 157
BsrBI CCGCTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 933
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 247, 546
BssMI GATC 4 cut(s) 115, 542, 698, 730
Bst2UI CCWGG 1 cut(s) 723
Bst6I CTCTTC 3 cut(s) 53, 237, 441
BstAPI GCANNNNNTGC 2 cut(s) 373, 401
BstC8I GCNNGC 2 cut(s) 357, 378
BstDEI CTNAG 2 cut(s) 340, 429
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 5 cut(s) 107, 243, 589, 812, 845
BstFNI CGCG 1 cut(s) 480
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 2 cut(s) 480, 503
BstKTI GATC 4 cut(s) 118, 545, 701, 733
BstMAI GTCTC 2 cut(s) 157, 343
BstMBI GATC 4 cut(s) 115, 542, 698, 730
BstMWI GCNNNNNNNGC 3 cut(s) 373, 401, 515
BstNI CCWGG 1 cut(s) 723
BstSCI CCNGG 1 cut(s) 721
BstSFI CTRYAG 1 cut(s) 349
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 2 cut(s) 367, 388
BstX2I RGATCY 3 cut(s) 542, 698, 730
BstXI CCANNNNNNTGG 2 cut(s) 254, 600
BstYI RGATCY 3 cut(s) 542, 698, 730
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 5 cut(s) 107, 243, 589, 812, 845
BveI ACCTGC 1 cut(s) 482
Cac8I GCNNGC 2 cut(s) 357, 378
CfoI GCGC 2 cut(s) 480, 503
Cfr10I RCCGGY 1 cut(s) 86
Csp6I GTAC 3 cut(s) 308, 682, 824
CviAII CATG 4 cut(s) 13, 396, 526, 884
CviQI GTAC 3 cut(s) 308, 682, 824
DdeI CTNAG 2 cut(s) 340, 429
DpnI GATC 4 cut(s) 117, 544, 700, 732
DpnII GATC 4 cut(s) 115, 542, 698, 730
Eam1104I CTCTTC 3 cut(s) 53, 237, 441
EarI CTCTTC 3 cut(s) 53, 237, 441
Eco24I GRGCYC 1 cut(s) 443
Eco31I GGTCTC 1 cut(s) 157
Eco47III AGCGCT 1 cut(s) 502
EcoRII CCWGG 1 cut(s) 721
EcoT22I ATGCAT 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 443
FaeI CATG 4 cut(s) 16, 399, 529, 887
FatI CATG 4 cut(s) 12, 395, 525, 883
Fnu4HI GCNGC 3 cut(s) 80, 381, 402
FokI GGATG 5 cut(s) 94, 230, 576, 819, 852
FriOI GRGCYC 1 cut(s) 443
Fsp4HI GCNGC 3 cut(s) 80, 381, 402
FspBI CTAG 2 cut(s) 311, 356
GlaI GCGC 2 cut(s) 479, 502
GluI GCNGC 3 cut(s) 80, 381, 402
GsuI CTGGAG 2 cut(s) 147, 525
HaeII RGCGCY 1 cut(s) 504
HapII CCGG 2 cut(s) 87, 174
HhaI GCGC 2 cut(s) 480, 503
Hin1II CATG 4 cut(s) 16, 399, 529, 887
Hin6I GCGC 2 cut(s) 478, 501
HinP1I GCGC 2 cut(s) 478, 501
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 4 cut(s) 129, 134, 626, 718
HpaII CCGG 2 cut(s) 87, 174
HphI GGTGA 4 cut(s) 76, 204, 506, 512
Hpy166II GTNNAC 2 cut(s) 241, 346
Hpy188I TCNGA 4 cut(s) 430, 445, 547, 703
Hpy188III TCNNGA 2 cut(s) 174, 793
Hpy8I GTNNAC 2 cut(s) 241, 346
HpyAV CCTTC 2 cut(s) 171, 430
HpyCH4IV ACGT 2 cut(s) 306, 822
HpyCH4V TGCA 6 cut(s) 275, 395, 491, 589, 647, 810
HpyF10VI GCNNNNNNNGC 3 cut(s) 373, 401, 515
HpyF3I CTNAG 2 cut(s) 340, 429
HpySE526I ACGT 2 cut(s) 306, 822
Hsp92II CATG 4 cut(s) 16, 399, 529, 887
HspAI GCGC 2 cut(s) 478, 501
Kpn2I TCCGGA 1 cut(s) 173
Kzo9I GATC 4 cut(s) 115, 542, 698, 730
LmnI GCTCC 2 cut(s) 446, 506
Lsp1109I GCAGC 2 cut(s) 367, 388
LweI GCATC 4 cut(s) 133, 382, 598, 797
MaeI CTAG 2 cut(s) 311, 356
MaeII ACGT 2 cut(s) 306, 822
MaeIII GTNAC 3 cut(s) 17, 494, 818
MalI GATC 4 cut(s) 117, 544, 700, 732
MbiI CCGCTC 1 cut(s) 79
MboI GATC 4 cut(s) 115, 542, 698, 730
MboII GAAGA 4 cut(s) 40, 224, 458, 635
MflI RGATCY 3 cut(s) 542, 698, 730
MhlI GDGCHC 2 cut(s) 369, 443
MluCI AATT 7 cut(s) 72, 285, 737, 746, 811, 939, 955
MlyI GAGTC 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 757
MnlI CCTC 9 cut(s) 54, 114, 197, 240, 245, 424, 541, 697, 850
Mph1103I ATGCAT 1 cut(s) 397
MroI TCCGGA 1 cut(s) 173
MseI TTAA 4 cut(s) 303, 564, 954, 958
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 2 cut(s) 87, 174
MspR9I CCNGG 1 cut(s) 723
MvaI CCWGG 1 cut(s) 723
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 3 cut(s) 373, 401, 515
NdeII GATC 4 cut(s) 115, 542, 698, 730
NheI GCTAGC 1 cut(s) 355
NlaIII CATG 4 cut(s) 16, 399, 529, 887
NlaIV GGNNCC 4 cut(s) 442, 544, 700, 732
NmuCI GTSAC 2 cut(s) 494, 818
NsiI ATGCAT 1 cut(s) 397
OliI CACNNNNGTG 1 cut(s) 252
PacI TTAATTAA 1 cut(s) 958
PaqCI CACCTGC 1 cut(s) 482
PfeI GAWTC 3 cut(s) 134, 626, 718
PkrI GCNGC 3 cut(s) 81, 382, 403
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PshBI ATTAAT 1 cut(s) 954
Psp6I CCWGG 1 cut(s) 721
PspGI CCWGG 1 cut(s) 721
PspN4I GGNNCC 4 cut(s) 442, 544, 700, 732
PsuI RGATCY 3 cut(s) 542, 698, 730
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 3 cut(s) 309, 683, 825
RsaNI GTAC 3 cut(s) 308, 682, 824
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 4 cut(s) 303, 564, 954, 958
SatI GCNGC 3 cut(s) 80, 381, 402
Sau3AI GATC 4 cut(s) 115, 542, 698, 730
SchI GAGTC 1 cut(s) 138
ScrFI CCNGG 1 cut(s) 723
SduI GDGCHC 2 cut(s) 369, 443
SfaNI GCATC 4 cut(s) 133, 382, 598, 797
SfcI CTRYAG 1 cut(s) 349
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 318
SmoI CTYRAG 1 cut(s) 318
SpeI ACTAGT 1 cut(s) 310
Sse9I AATT 7 cut(s) 72, 285, 737, 746, 811, 939, 955
SsiI CCGC 1 cut(s) 79
SspMI CTAG 2 cut(s) 311, 356
StyD4I CCNGG 1 cut(s) 721
TaiI ACGT 2 cut(s) 309, 825
TaqI TCGA 3 cut(s) 70, 132, 864
TasI AATT 7 cut(s) 72, 285, 737, 746, 811, 939, 955
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 3 cut(s) 134, 626, 718
Tru1I TTAA 4 cut(s) 303, 564, 954, 958
Tru9I TTAA 4 cut(s) 303, 564, 954, 958
TseFI GTSAC 2 cut(s) 494, 818
TseI GCWGC 2 cut(s) 380, 401
Tsp45I GTSAC 2 cut(s) 494, 818
TspDTI ATGAA 1 cut(s) 940
TspGWI ACGGA 1 cut(s) 264
VspI ATTAAT 1 cut(s) 954
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XspI CTAG 2 cut(s) 311, 356
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.