MD05G1076400.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000397
Physical Location & Seq
Reverse (-)
16336146 .. 16337120
975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1076400.v1.1.491

Sequence Viewer

Length: 975 bp
ATGGACTTCTCTTCATTGAATCCAAGCATGCGTAACGATATAGCCTATGATTTCTCTCCTCTCATCAAAATCTACAAAGATGGTCGAATTGAGCGGCTCACCGGCACAACCAAGCTTCCTCCATCCCTTGATCACAAAACTGGAGTCGAATCCAAAGACGCTGTGATCTCACAAGAACCAGCATTGTCCGTAAGGCTTTACCTCCCCACCAAATCCACCACCAACTCACCCGATAAAAAACTCCCTCTTCTTGTTTACATCCACGGAGGCGGTTTCGTGGTTGAAACTGCATTTTCTCCAATTTATCACACCCACCTTAACGTACTAGTTGCTCAGGCCAATGTTGTCGCTGTCTCGGTTGACTACAGGCTAGCCCCAGAGCACTCTCTGCCAGCTGCTTACAATGATGCATGGGCTGCTATCAAATGGGTTGCTTCCCATTCTGAGGGGAAGGGCTCCGAAGAGTGGCTAAATCGCCACGCAGATTTTCAGCGCGTGTTTTTTGCAGGTGACAGCGCTGGAGCTAACATAGCTCACCACATGGGATTGAGAGTAGGGTCCGAGGGGTTGAGTGGTGTTAAGCTGAATGGCATTGTGTTGGTGCATCCATATTTTTGGGGAACAGAGCCGATTGGGGCAGAATCGGCTCTGCCCCCTATTGCAAGAGAGTATATGGCTGGTTTGTGGCGTGTGGCGTACCCCTTGACAAGTGGATCCGATGACCCGTTTTTGAATCCTGGTAAGGATCCAAAATTGGGGGAATTAGGCTGTGAGAAAGTGCTGGTTTGTGTTGGAGAGAAAGATGTCTTGAAAGAAAGGGGATGCAATTATAGTGAGGTACTAAAAAAGAGTGGATGGAAAGGGGCTGTGGAGGTCATCGAAGCAAAGGGGGAGAACCATGTGTTCCATTTGTTCAATCCCACTTGTGACAATGCTGTGGCAATGTTCAAAAAAATTGTTTCTTTCATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

325

Amino Acids

35.45

Weight (kDa)

6.46

Isoelectric Point (pI)

28.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 52 - 183 2.2e-10 Carboxylesterase family
BD-FAE PF20434 77 - 187 1.2e-14 BD-FAE
Abhydrolase_3 PF07859 84 - 303 2.8e-55 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 497
Acc36I ACCTGC 1 cut(s) 497
AccBSI CCGCTC 1 cut(s) 94
AccII CGCG 1 cut(s) 495
AciI CCGC 2 cut(s) 94, 270
AclWI GGATC 4 cut(s) 708, 721, 740, 753
AfaI GTAC 3 cut(s) 324, 698, 840
AfeI AGCGCT 1 cut(s) 517
AfiI CCNNNNNNNGG 3 cut(s) 445, 465, 755
AgsI TTSAA 6 cut(s) 19, 284, 733, 811, 916, 949
AhlI ACTAGT 1 cut(s) 325
AjnI CCWGG 1 cut(s) 736
AluBI AGCT 5 cut(s) 115, 395, 524, 533, 583
AluI AGCT 5 cut(s) 115, 395, 524, 533, 583
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 4 cut(s) 708, 721, 740, 753
Aor51HI AGCGCT 1 cut(s) 517
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 2 cut(s) 395, 416
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
AseI ATTAAT 1 cut(s) 969
AspLEI GCGC 2 cut(s) 495, 518
AspS9I GGNCC 1 cut(s) 558
AsuHPI GGTGA 4 cut(s) 91, 219, 521, 527
AsuNHI GCTAGC 1 cut(s) 370
AvaII GGWCC 1 cut(s) 558
BamHI GGATCC 2 cut(s) 713, 745
BanII GRGCYC 1 cut(s) 458
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 382, 403
BccI CCATC 3 cut(s) 74, 130, 849
BciT130I CCWGG 1 cut(s) 738
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 358
BcuI ACTAGT 1 cut(s) 325
BfaI CTAG 2 cut(s) 326, 371
BfmI CTRYAG 1 cut(s) 364
BfoI RGCGCY 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 497
BisI GCNGC 3 cut(s) 95, 396, 417
BlsI GCNGC 3 cut(s) 96, 397, 418
Bme1390I CCNGG 1 cut(s) 738
Bme18I GGWCC 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 4 cut(s) 457, 559, 715, 747
BmrFI CCNGG 1 cut(s) 738
BmsI GCATC 3 cut(s) 397, 613, 812
BmtI GCTAGC 1 cut(s) 374
BpmI CTGGAG 2 cut(s) 162, 540
Bpu10I CCTNAGC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 262, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 445, 465, 755
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 948
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 2 cut(s) 262, 561
BseGI GGATG 5 cut(s) 122, 258, 604, 827, 860
BseLI CCNNNNNNNGG 3 cut(s) 445, 465, 755
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 2 cut(s) 347, 435
BseNI ACTGG 1 cut(s) 145
BseRI GAGGAG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 382, 403
Bsh1236I CGCG 1 cut(s) 495
BshFI GGCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 1 cut(s) 102
BslI CCNNNNNNNGG 3 cut(s) 445, 465, 755
BsmAI GTCTC 1 cut(s) 358
BsnI GGCC 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 384, 458
Bsp143I GATC 4 cut(s) 130, 165, 713, 745
BspACI CCGC 2 cut(s) 94, 270
BspANI GGCC 1 cut(s) 338
BspCNI CTCAG 2 cut(s) 346, 436
BspFNI CGCG 1 cut(s) 495
BspLI GGNNCC 4 cut(s) 457, 559, 715, 747
BspMI ACCTGC 1 cut(s) 497
BspOI GCTAGC 1 cut(s) 374
BspPI GGATC 4 cut(s) 708, 721, 740, 753
BsrBI CCGCTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 948
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 262, 561
BssMI GATC 4 cut(s) 130, 165, 713, 745
Bst2UI CCWGG 1 cut(s) 738
Bst6I CTCTTC 3 cut(s) 16, 252, 456
BstAPI GCANNNNNTGC 2 cut(s) 388, 416
BstC8I GCNNGC 3 cut(s) 29, 372, 393
BstDEI CTNAG 2 cut(s) 333, 444
BstDSI CCRYGG 1 cut(s) 262
BstF5I GGATG 5 cut(s) 122, 258, 604, 827, 860
BstFNI CGCG 1 cut(s) 495
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 2 cut(s) 495, 518
BstKTI GATC 4 cut(s) 133, 168, 716, 748
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 4 cut(s) 130, 165, 713, 745
BstMWI GCNNNNNNNGC 4 cut(s) 388, 416, 530, 644
BstNI CCWGG 1 cut(s) 738
BstNSI RCATGY 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 736
BstSFI CTRYAG 1 cut(s) 364
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 2 cut(s) 382, 403
BstX2I RGATCY 2 cut(s) 713, 745
BstXI CCANNNNNNTGG 1 cut(s) 615
BstYI RGATCY 2 cut(s) 713, 745
BsuRI GGCC 1 cut(s) 338
BtgI CCRYGG 1 cut(s) 262
BtsCI GGATG 5 cut(s) 122, 258, 604, 827, 860
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 3 cut(s) 29, 372, 393
CfoI GCGC 2 cut(s) 495, 518
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 558
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 323, 697, 839
CviAII CATG 4 cut(s) 28, 411, 541, 899
CviQI GTAC 3 cut(s) 323, 697, 839
DdeI CTNAG 2 cut(s) 333, 444
DpnI GATC 4 cut(s) 132, 167, 715, 747
DpnII GATC 4 cut(s) 130, 165, 713, 745
Eam1104I CTCTTC 3 cut(s) 16, 252, 456
EarI CTCTTC 3 cut(s) 16, 252, 456
Eco24I GRGCYC 1 cut(s) 458
Eco47I GGWCC 1 cut(s) 558
Eco47III AGCGCT 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 736
EcoT22I ATGCAT 1 cut(s) 412
EcoT38I GRGCYC 1 cut(s) 458
FaeI CATG 4 cut(s) 31, 414, 544, 902
FatI CATG 4 cut(s) 27, 410, 540, 898
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 95, 396, 417
FokI GGATG 5 cut(s) 109, 245, 591, 834, 867
FriOI GRGCYC 1 cut(s) 458
Fsp4HI GCNGC 3 cut(s) 95, 396, 417
FspBI CTAG 2 cut(s) 326, 371
GlaI GCGC 2 cut(s) 494, 517
GluI GCNGC 3 cut(s) 95, 396, 417
GsuI CTGGAG 2 cut(s) 162, 540
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 1 cut(s) 338
HapII CCGG 1 cut(s) 102
HgaI GACGC 1 cut(s) 167
HhaI GCGC 2 cut(s) 495, 518
Hin1II CATG 4 cut(s) 31, 414, 544, 902
Hin6I GCGC 2 cut(s) 493, 516
HinP1I GCGC 2 cut(s) 493, 516
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 5 cut(s) 19, 144, 149, 641, 733
HpaII CCGG 1 cut(s) 102
HphI GGTGA 4 cut(s) 91, 219, 521, 527
Hpy166II GTNNAC 2 cut(s) 256, 361
Hpy188I TCNGA 4 cut(s) 445, 460, 562, 718
Hpy188III TCNNGA 1 cut(s) 808
Hpy8I GTNNAC 2 cut(s) 256, 361
HpyAV CCTTC 1 cut(s) 445
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 6 cut(s) 290, 410, 506, 604, 662, 825
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 416, 530, 644
HpyF3I CTNAG 2 cut(s) 333, 444
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 4 cut(s) 31, 414, 544, 902
HspAI GCGC 2 cut(s) 493, 516
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 4 cut(s) 130, 165, 713, 745
LmnI GCTCC 2 cut(s) 461, 521
Lsp1109I GCAGC 2 cut(s) 382, 403
LweI GCATC 3 cut(s) 397, 613, 812
MaeI CTAG 2 cut(s) 326, 371
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 3 cut(s) 32, 509, 926
MalI GATC 4 cut(s) 132, 167, 715, 747
MbiI CCGCTC 1 cut(s) 94
MboI GATC 4 cut(s) 130, 165, 713, 745
MboII GAAGA 3 cut(s) 3, 239, 473
MflI RGATCY 2 cut(s) 713, 745
MhlI GDGCHC 2 cut(s) 384, 458
MluCI AATT 7 cut(s) 87, 300, 752, 761, 826, 954, 970
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 772
MnlI CCTC 9 cut(s) 69, 129, 212, 255, 260, 439, 556, 829, 865
Mph1103I ATGCAT 1 cut(s) 412
MseI TTAA 4 cut(s) 318, 579, 969, 973
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 1 cut(s) 738
MvaI CCWGG 1 cut(s) 738
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 4 cut(s) 388, 416, 530, 644
NdeII GATC 4 cut(s) 130, 165, 713, 745
NheI GCTAGC 1 cut(s) 370
NlaIII CATG 4 cut(s) 31, 414, 544, 902
NlaIV GGNNCC 4 cut(s) 457, 559, 715, 747
NmuCI GTSAC 2 cut(s) 509, 926
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 1 cut(s) 31
PacI TTAATTAA 1 cut(s) 973
PaeI GCATGC 1 cut(s) 31
PaqCI CACCTGC 1 cut(s) 497
PfeI GAWTC 4 cut(s) 19, 149, 641, 733
PkrI GCNGC 3 cut(s) 96, 397, 418
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PshBI ATTAAT 1 cut(s) 969
Psp6I CCWGG 1 cut(s) 736
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 4 cut(s) 457, 559, 715, 747
PspPI GGNCC 1 cut(s) 558
PsuI RGATCY 2 cut(s) 713, 745
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 3 cut(s) 324, 698, 840
RsaNI GTAC 3 cut(s) 323, 697, 839
SaqAI TTAA 4 cut(s) 318, 579, 969, 973
SatI GCNGC 3 cut(s) 95, 396, 417
Sau3AI GATC 4 cut(s) 130, 165, 713, 745
Sau96I GGNCC 1 cut(s) 558
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 1 cut(s) 738
SduI GDGCHC 2 cut(s) 384, 458
SfaNI GCATC 3 cut(s) 397, 613, 812
SfcI CTRYAG 1 cut(s) 364
SinI GGWCC 1 cut(s) 558
SpeI ACTAGT 1 cut(s) 325
SphI GCATGC 1 cut(s) 31
Sse9I AATT 7 cut(s) 87, 300, 752, 761, 826, 954, 970
SsiI CCGC 2 cut(s) 94, 270
SspMI CTAG 2 cut(s) 326, 371
StyD4I CCNGG 1 cut(s) 736
TaiI ACGT 1 cut(s) 324
TaqI TCGA 3 cut(s) 85, 147, 879
TasI AATT 7 cut(s) 87, 300, 752, 761, 826, 954, 970
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 4 cut(s) 19, 149, 641, 733
Tru1I TTAA 4 cut(s) 318, 579, 969, 973
Tru9I TTAA 4 cut(s) 318, 579, 969, 973
TseFI GTSAC 2 cut(s) 509, 926
TseI GCWGC 2 cut(s) 395, 416
Tsp45I GTSAC 2 cut(s) 509, 926
TspDTI ATGAA 1 cut(s) 955
TspGWI ACGGA 2 cut(s) 178, 279
VpaK11BI GGWCC 1 cut(s) 558
VspI ATTAAT 1 cut(s) 969
XceI RCATGY 1 cut(s) 31
XspI CTAG 2 cut(s) 326, 371
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.