Rorug05G0580600

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000397
Physical Location & Seq
Reverse (-)
77749327 .. 77757426
8100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0580600.1

Sequence Viewer

Length: 3360 bp
ATGACCACTCAATTTGGGGCCACTTCTTCTTCCTCCCTCTCTTCCTCTTCTGGTACCATTCAGCCCTCTTTCTCTTCCTCTTGTGCTACCATTCAGTCATGGACATACCATGTCTTTTTGAGTTTTAGAGGAGAGGATACGCGCAACACTTTTACAGGCCATTTGCACAAAAATTTGGTTCAGAAGGGAATTAAAACCTTCATAGATGATGGCCTTACAAGAGGAGAAGAAATATCAAGCGCACTTTTTAAAGCAATTGACGAGTCAAAAATTTCTGTCATTGTGTTCTCTGAAAGTTATGCTTCATCAAAATGGTGTTTAGAAGAACTTGTGAAGATCATGCATTGTAATGAATCGAAGCAACAAATTGTATACCCTATTTTTTACAAGGTAGATCCATCAGATGTACGAAACCAAGAGGGTAGTTTTGGTCAGGCACTTGCTCATCATGATAACTTAGAGAAGGTGGCAGGATGGAAGACAGCTCTTACAAAAGCAGGAAATCTTTCTGGGTGGCATTTCTCACACGGAGGGCATGAATCTAAATTCATTGATAAAATTGTTGAAGAGATCTCAAAACAATTAGTTTTAAATTTTACATATTTGAATGGGGACAAATACTTAGTTGGAATAGAGTCTCGTTTAAAACATATGGAAGAGCTTTTATGTGTTGGAGAAAGTGACGTACGAATGATAGGGATATGGGGCATTGGTGGATTAGGCAAAACAACAATTGCAAAGACTGTTTATAATATAATTTCCCATAAATTTGAAGGCAATTGTTTTTTGGAAAATGTTAGAGAAAGATCGTTGAAATATGGAGGCCTACAAGAACTACAGACGTTTCTTCTTTCTAAGACTCTGGGGTTGAGAGACTTAGAGGTAAATAGTCTTGACGAAGGAAGCATCATGATAAAAGAAAGGTTAAGCCATAGGAGAGTTTTCTTAGTTGTTGATGATGTGAATGATTTGGACCAGTTAAAAAGTTTAGTCGGAAAGTCTAATTGGTTTGGTTCTGGTAGTAGAATAATCATAACAACACGAGATAAGCACTTGCTACATGATGTTAATCACATATACCAAGTGGAGAAGTTAAGTCACAGTGAAGCTTTGGAGCTCTTCAACTTTAATGCATTCAAAGGAAAAGGACAGATGGGTGATTACGTTGAACTCATAGATAATGTAATATGCTATGCTGAAGGGCTTCCTCTAGTTGTAGAAGTTATGGGTTCATATCTACATGGTAGAAGTACAGACACGTGGAAAGATGCATTAGAGTCTTGCAAAAGAGATCCTAAGTTTCAACAAGTGCTCAAATTAAGTTATGATGCACTTGAACCTTCGATGAAGGAAGTTTTTCTTCACATCGCATGTTTCTTTAAAGGTAGAAATAAGAACTATGTGATGGACATATTAGAAGGATGTGACCTACGACCTAAACACAGCATTGAAGTACTCATAGAAAAGGCCCTCATAAGCATTACTGAAGCAAATGGTGTTTGGATGCATGATGTGCTAGAAGAAATGGGCAAAGAAATAGTTTGTCAAGAGTCATCAGAGCCAGGAGAACGTAGCAGATTGTGGTTGTACGAAGATGTTTGTCACGTTTTTGTGCACAACACTGGAACAAGCAAAGTTAAAGGGATCATGGTTCTGAAAGGCGTACCAGATCAACAAATACCCTTGAATAAGAAAAGCTTCTTAAAGTTAAACGGTCTTCAAATTCTTATAATTTGTGGTGATGTATTCTATGGAGATCATGTGGATTACCTTCCCAATGATTTGAGACTCTTAAACTGGAAGGATTGTCAGTTACAATATTTTCCATCAGATTTTTATCCTAGGAAACTTGCTGCACTCAAGATGGATTTCAGTGAGATATCATCACCATGGAGTGTACTTAAGGGAGGTTTTCTACGTCAAATGACTCGGATCTGCACATCACCATTGGGGAAGAGACTACAGAATATGCACAATTTGAAATCAGTCGATTTGGGAAATTGCAATGGTGTAACAACGTTTTCCTACCTCTCTAGATTGCCAAACTTAGAGGAGTTGAAAATAAGAGACAATAAAAAAGTCAAGAAGTTTGAAATTGTGGAAGAGATGAAATCCTTGAAAAGGTTGGATCTAAGCCGTACTGCAATCAGAGAATTGGCTTCATCAACAATCAGATGTCTCATTAATCTGGAAGAGTTAACTTTATCATATTGCCATAAACTTAAACATGTGCCGTGCAGCATTTTTGAGTTGCAACATCTAGAGGTTTTGGATCTCTCTTATTGTAAAAAGCTGGTAACATTTCCAACAAAGTCAGGGTTTTCAACGGGATCGACCAGTTTACAGGACAAGCACTATACTCCATTATTCGTCAGTTTGAACAATTGCGGGCGTCTTGTAGAAATTGCAGAAATTCCGCGAGAAAGATATGACCTAGATGCGAGTGGCTGCTATGGATTGCAAAAAATTTCAAAATTGTCAAACATTTTGGAAGGTAAAGAGTCAAAATTGACCCCTTTTATGGACTTGCTTAATTGCTACGAACTGTCCGGCAATCTGGCTCGTGACGTGGCAAAGGTGAAAAAGAATTTTCCGGATAATTCCAGGTCAACAGCTCTGTGGTTTCTCGTTTTCTCATGTCGGCAATCTGAGTGTCAGGTTTTCTTTAACGGCCCGCCTTACAAATTTGGGATTCCGGAGTGGTTCACTTGGCGCAGGGATTTCGATATGGAAGGACTTCAAGGATGTGAAGAATTTAGAATTGATTTTCCTGGAAATTTCAAATGGGAGGACAAAGGATTGGCTTTCTGTGCTCAAACTGTTGATAACGATCACCAGCGCTGGGCTATGAGATCATGTTTCAGAATAGTCACAATCTACGTTAACGGTGTATGCATCACTGAAGTATACCTAATGACGGGATGGTCCGTGTGGGGATACTTGCCATTCGATACTATTATAGAACGGCTGAGTGAGAGTGGGTCACCACCACCTTCCGTGTGTCTGGTTAAGATTGAATTTAAAACCCGCGATGTATGGTCTAACGAGATTAAGGACGAAGGGCCCCCGATGCAAAGCTGCGGAGTCCACGTTGTAATGCCAGAGGATGAAGGCCAATTTGTTTTGAGCTTATCGACCCCTTCAGTCAATCTTGAGTTCCTCTTTGCTTTTAATGGTATAGAGGTTTTCCGGTCCTCTTGGCACTTAAAGTTACTTAATAGGATGCTACAGATTAGGCCCTGGAAATTTTACGTTGCTAAAACAAAGAAAATGTGGGCTGATATGATATTCTCCTGTGGAGGAAGGGATCTGGTGCTGACACTAATATGGGGTACTAAACCTGATGCTTTGTTGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

1119

Amino Acids

127.52

Weight (kDa)

6.79

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 36 - 204 8.5e-57 TIR domain
TIR_2 PF13676 38 - 133 9.8e-14 TIR domain
NB-ARC PF00931 219 - 381 5.1e-16 NB-ARC domain
WHD_ROQ1 PF23282 450 - 518 8.3e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_RPS2 PF23247 642 - 767 5.5e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 750, 1730
AasI GACNNNNNNGTC 1 cut(s) 2923
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 53
AccI GTMKAC 2 cut(s) 372, 2907
AccII CGCG 3 cut(s) 142, 2419, 3030
AccIII TCCGGA 2 cut(s) 2593, 2695
AciI CCGC 5 cut(s) 2388, 2417, 2675, 3028, 3081
AclI AACGTT 1 cut(s) 2018
AclWI GGATC 8 cut(s) 389, 1286, 1652, 1940, 2136, 2280, 2338, 3315
AcuI CTGAAG 4 cut(s) 1218, 1506, 2922, 3126
AcvI CACGTG 1 cut(s) 1260
AcyI GRCGYC 1 cut(s) 2392
AfeI AGCGCT 1 cut(s) 2840
AfiI CCNNNNNNNGG 4 cut(s) 2121, 2521, 2842, 2917
AflII CTTAAG 1 cut(s) 1901
AflIII ACRYGT 2 cut(s) 1257, 2227
AjiI CACGTC 1 cut(s) 2569
AjnI CCWGG 4 cut(s) 1561, 2603, 2770, 3239
AjuI GAANNNNNNNTTGG 2 cut(s) 988, 1020
AluBI AGCT 9 cut(s) 485, 661, 1109, 1117, 1698, 2293, 2615, 3078, 3129
AluI AGCT 9 cut(s) 485, 661, 1109, 1117, 1698, 2293, 2615, 3078, 3129
Alw21I GWGCWC 4 cut(s) 1119, 1314, 1617, 2815
Alw26I GTCTC 6 cut(s) 642, 867, 1780, 1951, 2061, 2183
Alw44I GTGCAC 1 cut(s) 1613
AlwI GGATC 8 cut(s) 389, 1286, 1652, 1940, 2136, 2280, 2338, 3315
Aor13HI TCCGGA 2 cut(s) 2593, 2695
Aor51HI AGCGCT 1 cut(s) 2840
AoxI GGCC 9 cut(s) 18, 157, 211, 823, 1467, 2671, 3062, 3112, 3236
ApaI GGGCCC 1 cut(s) 3066
ApaLI GTGCAC 1 cut(s) 1613
ApeKI GCWGC 4 cut(s) 1853, 2238, 2448, 3078
AseI ATTAAT 1 cut(s) 2184
Asp700I GAANNNNTTC 5 cut(s) 505, 1203, 1356, 1697, 2736
Asp718I GGTACC 1 cut(s) 53
AspA2I CCTAGG 1 cut(s) 1841
AspLEI GCGC 4 cut(s) 144, 242, 2715, 2841
AspS9I GGNCC 9 cut(s) 18, 973, 1468, 2672, 2925, 3062, 3063, 3192, 3237
AsuHPI GGTGA 7 cut(s) 1169, 1751, 1878, 1935, 2590, 2825, 2976
AvaII GGWCC 3 cut(s) 973, 2925, 3192
AvrII CCTAGG 1 cut(s) 1841
BaeGI GKGCMC 2 cut(s) 1617, 3066
BanI GGYRCC 1 cut(s) 53
BanII GRGCYC 2 cut(s) 1119, 3066
BauI CACGAG 2 cut(s) 1041, 2562
BbrPI CACGTG 1 cut(s) 1260
BbsI GAAGAC 2 cut(s) 485, 1709
Bbv12I GWGCWC 4 cut(s) 1119, 1314, 1617, 2815
BbvI GCAGC 4 cut(s) 1840, 2250, 2435, 3065
BccI CCATC 8 cut(s) 203, 406, 468, 1147, 1399, 1834, 1858, 2916
BceAI ACGGC 4 cut(s) 2121, 2218, 2686, 2981
BcgI CGANNNNNNTGC 2 cut(s) 2704, 2738
BciT130I CCWGG 4 cut(s) 1563, 2605, 2772, 3241
BciVI GTATCC 2 cut(s) 130, 2930
BcoDI GTCTC 6 cut(s) 642, 867, 1780, 1951, 2061, 2183
BfaI CTAG 6 cut(s) 1211, 1517, 1842, 2034, 2261, 2435
BfmI CTRYAG 3 cut(s) 836, 1961, 3227
BfoI RGCGCY 1 cut(s) 2842
BfrI CTTAAG 1 cut(s) 1901
BfuI GTATCC 2 cut(s) 130, 2930
BglII AGATCT 1 cut(s) 570
BisI GCNGC 4 cut(s) 1854, 2239, 2449, 3079
BlnI CCTAGG 1 cut(s) 1841
BlsI GCNGC 4 cut(s) 1855, 2240, 2450, 3080
BmcAI AGTACT 1 cut(s) 1455
Bme1390I CCNGG 4 cut(s) 1563, 2605, 2772, 3241
Bme18I GGWCC 3 cut(s) 973, 2925, 3192
BmgBI CACGTC 1 cut(s) 2569
BmgT120I GGNCC 9 cut(s) 18, 973, 1468, 2672, 2925, 3062, 3063, 3192, 3237
BmiI GGNNCC 4 cut(s) 19, 55, 3064, 3065
BmrFI CCNGG 4 cut(s) 1563, 2605, 2772, 3241
BmsI GCATC 9 cut(s) 915, 1258, 1318, 1494, 2428, 2904, 3060, 3213, 3334
BpiI GAAGAC 2 cut(s) 485, 1709
BpuEI CTTGAG 2 cut(s) 1844, 3173
BsaAI YACGTR 1 cut(s) 1260
BsaBI GATNNNNATC 3 cut(s) 1068, 1836, 2829
BsaHI GRCGYC 1 cut(s) 2392
BsaJI CCNNGG 3 cut(s) 1841, 1889, 3239
BsaWI WCCGGW 3 cut(s) 2593, 2695, 3189
BsaXI ACNNNNNCTCC 4 cut(s) 1746, 1776, 3075, 3105
Bsc4I CCNNNNNNNGG 4 cut(s) 2121, 2521, 2842, 2917
Bse1I ACTGG 4 cut(s) 976, 1627, 1802, 2337
Bse3DI GCAATG 1 cut(s) 2011
Bse8I GATNNNNATC 3 cut(s) 1068, 1836, 2829
BseAI TCCGGA 2 cut(s) 2593, 2695
BseBI CCWGG 4 cut(s) 1563, 2605, 2772, 3241
BseDI CCNNGG 3 cut(s) 1841, 1889, 3239
BseGI GGATG 7 cut(s) 479, 1427, 1509, 2750, 2927, 3112, 3228
BseJI GATNNNNATC 3 cut(s) 1068, 1836, 2829
BseLI CCNNNNNNNGG 4 cut(s) 2121, 2521, 2842, 2917
BseMI GCAATG 1 cut(s) 2011
BseMII CTCAG 2 cut(s) 2640, 2960
BseNI ACTGG 4 cut(s) 976, 1627, 1802, 2337
BseRI GAGGAG 3 cut(s) 144, 237, 2066
BseSI GKGCMC 2 cut(s) 1617, 3066
BseXI GCAGC 4 cut(s) 1840, 2250, 2435, 3065
BseYI CCCAGC 1 cut(s) 2841
BsgI GTGCAG 3 cut(s) 1839, 1921, 2257
Bsh1236I CGCG 3 cut(s) 142, 2419, 3030
BshFI GGCC 9 cut(s) 20, 159, 213, 825, 1469, 2673, 3064, 3114, 3238
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 4 cut(s) 1119, 1314, 1617, 2815
BsiSI CCGG 4 cut(s) 2550, 2594, 2696, 3190
BsiWI CGTACG 1 cut(s) 685
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 4 cut(s) 2121, 2521, 2842, 2917
BsmAI GTCTC 6 cut(s) 642, 867, 1780, 1951, 2061, 2183
BsmFI GGGAC 1 cut(s) 626
BsmI GAATGC 1 cut(s) 1133
BsnI GGCC 9 cut(s) 20, 159, 213, 825, 1469, 2673, 3064, 3114, 3238
Bsp120I GGGCCC 1 cut(s) 3062
Bsp1286I GDGCHC 5 cut(s) 1119, 1314, 1617, 2815, 3066
Bsp13I TCCGGA 2 cut(s) 2593, 2695
Bsp19I CCATGG 1 cut(s) 1889
BspACI CCGC 5 cut(s) 2388, 2417, 2675, 3028, 3081
BspANI GGCC 9 cut(s) 20, 159, 213, 825, 1469, 2673, 3064, 3114, 3238
BspCNI CTCAG 2 cut(s) 2641, 2961
BspEI TCCGGA 2 cut(s) 2593, 2695
BspFNI CGCG 3 cut(s) 142, 2419, 3030
BspHI TCATGA 2 cut(s) 448, 909
BspLI GGNNCC 4 cut(s) 19, 55, 3064, 3065
BspPI GGATC 8 cut(s) 389, 1286, 1652, 1940, 2136, 2280, 2338, 3315
BspQI GCTCTTC 2 cut(s) 651, 1124
BspT107I GGYRCC 1 cut(s) 53
BspTI CTTAAG 1 cut(s) 1901
BsrDI GCAATG 1 cut(s) 2011
BsrI ACTGG 4 cut(s) 976, 1627, 1802, 2337
BssECI CCNNGG 3 cut(s) 1841, 1889, 3239
BssNAI GTATAC 2 cut(s) 373, 2908
BssNI GRCGYC 1 cut(s) 2392
BssSI CACGAG 2 cut(s) 1041, 2562
BssT1I CCWWGG 2 cut(s) 1841, 1889
Bst1107I GTATAC 2 cut(s) 373, 2908
Bst2BI CACGAG 2 cut(s) 1041, 2562
Bst2UI CCWGG 4 cut(s) 1563, 2605, 2772, 3241
Bst4CI ACNGT 6 cut(s) 745, 1103, 1715, 2547, 2821, 2888
Bst6I CTCTTC 9 cut(s) 46, 52, 79, 561, 651, 1124, 1949, 2097, 2187
BstACI GRCGYC 1 cut(s) 2392
BstAFI CTTAAG 1 cut(s) 1901
BstAPI GCANNNNNTGC 1 cut(s) 1513
BstBAI YACGTR 1 cut(s) 1260
BstC8I GCNNGC 2 cut(s) 2390, 2675
BstDSI CCRYGG 1 cut(s) 1889
BstEII GGTNACC 1 cut(s) 2982
BstENI CCTNNNNNAGG 1 cut(s) 2119
BstF5I GGATG 7 cut(s) 479, 1427, 1509, 2750, 2927, 3112, 3228
BstFNI CGCG 3 cut(s) 142, 2419, 3030
BstH2I RGCGCY 1 cut(s) 2842
BstHHI GCGC 4 cut(s) 144, 242, 2715, 2841
BstMAI GTCTC 6 cut(s) 642, 867, 1780, 1951, 2061, 2183
BstMWI GCNNNNNNNGC 3 cut(s) 1513, 2810, 3070
BstNI CCWGG 4 cut(s) 1563, 2605, 2772, 3241
BstNSI RCATGY 2 cut(s) 1374, 2231
BstPI GGTNACC 1 cut(s) 2982
BstSCI CCNGG 4 cut(s) 1561, 2603, 2770, 3239
BstSFI CTRYAG 3 cut(s) 836, 1961, 3227
BstSLI GKGCMC 2 cut(s) 1617, 3066
BstUI CGCG 3 cut(s) 142, 2419, 3030
BstV1I GCAGC 4 cut(s) 1840, 2250, 2435, 3065
BstV2I GAAGAC 2 cut(s) 485, 1709
BstX2I RGATCY 7 cut(s) 394, 570, 1291, 1932, 2128, 2272, 3307
BstYI RGATCY 7 cut(s) 394, 570, 1291, 1932, 2128, 2272, 3307
BstZ17I GTATAC 2 cut(s) 373, 2908
BsuI GTATCC 2 cut(s) 130, 2930
BsuRI GGCC 9 cut(s) 20, 159, 213, 825, 1469, 2673, 3064, 3114, 3238
BtgI CCRYGG 1 cut(s) 1889
BtgZI GCGATG 2 cut(s) 1351, 3045
BtrI CACGTC 1 cut(s) 2569
BtsCI GGATG 7 cut(s) 479, 1427, 1509, 2750, 2927, 3112, 3228
BtsIMutI CAGTG 4 cut(s) 1108, 1620, 1879, 2898
Cac8I GCNNGC 2 cut(s) 2390, 2675
CciI TCATGA 2 cut(s) 448, 909
CfoI GCGC 4 cut(s) 144, 242, 2715, 2841
Cfr13I GGNCC 9 cut(s) 18, 973, 1468, 2672, 2925, 3062, 3063, 3192, 3237
CseI GACGC 1 cut(s) 2381
CspCI CAANNNNNGTGG 1 cut(s) 30
DraI TTTAAA 5 cut(s) 250, 591, 645, 1381, 3022
DrdI GACNNNNNNGTC 1 cut(s) 2923
DseDI GACNNNNNNGTC 1 cut(s) 2923
Eam1104I CTCTTC 9 cut(s) 46, 52, 79, 561, 651, 1124, 1949, 2097, 2187
EarI CTCTTC 9 cut(s) 46, 52, 79, 561, 651, 1124, 1949, 2097, 2187
Ecl136II GAGCTC 1 cut(s) 1117
Eco130I CCWWGG 2 cut(s) 1841, 1889
Eco147I AGGCCT 1 cut(s) 825
Eco24I GRGCYC 2 cut(s) 1119, 3066
Eco32I GATATC 1 cut(s) 1881
Eco47I GGWCC 3 cut(s) 973, 2925, 3192
Eco47III AGCGCT 1 cut(s) 2840
Eco53kI GAGCTC 1 cut(s) 1117
Eco57I CTGAAG 4 cut(s) 1218, 1506, 2922, 3126
Eco72I CACGTG 1 cut(s) 1260
Eco91I GGTNACC 1 cut(s) 2982
EcoICRI GAGCTC 1 cut(s) 1117
EcoNI CCTNNNNNAGG 1 cut(s) 2119
EcoO109I RGGNCCY 4 cut(s) 1468, 3062, 3063, 3237
EcoO65I GGTNACC 1 cut(s) 2982
EcoRII CCWGG 4 cut(s) 1561, 2603, 2770, 3239
EcoRV GATATC 1 cut(s) 1881
EcoT14I CCWWGG 2 cut(s) 1841, 1889
EcoT22I ATGCAT 5 cut(s) 345, 1135, 1273, 1509, 2897
EcoT38I GRGCYC 2 cut(s) 1119, 3066
ErhI CCWWGG 2 cut(s) 1841, 1889
FalI AAGNNNNNCTT 6 cut(s) 286, 318, 1344, 1376, 1682, 1714
FaqI GGGAC 1 cut(s) 626
FauI CCCGC 3 cut(s) 2381, 2682, 3035
FauNDI CATATG 1 cut(s) 651
FblI GTMKAC 2 cut(s) 372, 2907
Fnu4HI GCNGC 4 cut(s) 1854, 2239, 2449, 3079
FokI GGATG 7 cut(s) 486, 1434, 1516, 2757, 2934, 3119, 3235
FriOI GRGCYC 2 cut(s) 1119, 3066
Fsp4HI GCNGC 4 cut(s) 1854, 2239, 2449, 3079
FspBI CTAG 6 cut(s) 1211, 1517, 1842, 2034, 2261, 2435
GlaI GCGC 4 cut(s) 143, 241, 2714, 2840
GluI GCNGC 4 cut(s) 1854, 2239, 2449, 3079
GsaI CCCAGC 1 cut(s) 2845
HaeII RGCGCY 1 cut(s) 2842
HaeIII GGCC 9 cut(s) 20, 159, 213, 825, 1469, 2673, 3064, 3114, 3238
HapII CCGG 4 cut(s) 2550, 2594, 2696, 3190
HgaI GACGC 1 cut(s) 2381
HhaI GCGC 4 cut(s) 144, 242, 2715, 2841
Hin1I GRCGYC 1 cut(s) 2392
Hin6I GCGC 4 cut(s) 142, 240, 2713, 2839
HinP1I GCGC 4 cut(s) 142, 240, 2713, 2839
HincII GTYRAC 3 cut(s) 2199, 2610, 2884
HindII GTYRAC 3 cut(s) 2199, 2610, 2884
HindIII AAGCTT 2 cut(s) 1107, 1696
HpaI GTTAAC 2 cut(s) 2199, 2884
HpaII CCGG 4 cut(s) 2550, 2594, 2696, 3190
HphI GGTGA 7 cut(s) 1169, 1751, 1878, 1935, 2590, 2825, 2976
HpyCH4III ACNGT 6 cut(s) 745, 1103, 1715, 2547, 2821, 2888
HpyF10VI GCNNNNNNNGC 3 cut(s) 1513, 2810, 3070
Hsp92I GRCGYC 1 cut(s) 2392
HspAI GCGC 4 cut(s) 142, 240, 2713, 2839
Kpn2I TCCGGA 2 cut(s) 2593, 2695
KpnI GGTACC 1 cut(s) 57
KspAI GTTAAC 2 cut(s) 2199, 2884
LguI GCTCTTC 2 cut(s) 651, 1124
LmnI GCTCC 1 cut(s) 1114
Lsp1109I GCAGC 4 cut(s) 1840, 2250, 2435, 3065
LweI GCATC 9 cut(s) 915, 1258, 1318, 1494, 2428, 2904, 3060, 3213, 3334
MaeI CTAG 6 cut(s) 1211, 1517, 1842, 2034, 2261, 2435
MfeI CAATTG 4 cut(s) 255, 732, 778, 2383
MflI RGATCY 7 cut(s) 394, 570, 1291, 1932, 2128, 2272, 3307
MhlI GDGCHC 5 cut(s) 1119, 1314, 1617, 2815, 3066
MlyI GAGTC 9 cut(s) 272, 644, 853, 1286, 1559, 1782, 1921, 2510, 3093
MmeI TCCRAC 5 cut(s) 607, 652, 973, 2106, 2330
Mph1103I ATGCAT 5 cut(s) 345, 1135, 1273, 1509, 2897
MroI TCCGGA 2 cut(s) 2593, 2695
MroXI GAANNNNTTC 5 cut(s) 505, 1203, 1356, 1697, 2736
MslI CAYNNNNRTG 4 cut(s) 310, 348, 1888, 3325
MspCI CTTAAG 1 cut(s) 1901
MspI CCGG 4 cut(s) 2550, 2594, 2696, 3190
MspR9I CCNGG 4 cut(s) 1563, 2605, 2772, 3241
MunI CAATTG 4 cut(s) 255, 732, 778, 2383
Mva1269I GAATGC 1 cut(s) 1133
MvaI CCWGG 4 cut(s) 1563, 2605, 2772, 3241
MvnI CGCG 3 cut(s) 142, 2419, 3030
MwoI GCNNNNNNNGC 3 cut(s) 1513, 2810, 3070
NcoI CCATGG 1 cut(s) 1889
NdeI CATATG 1 cut(s) 651
NlaIV GGNNCC 4 cut(s) 19, 55, 3064, 3065
NmuCI GTSAC 7 cut(s) 680, 1097, 1424, 1601, 2564, 2869, 2982
NsiI ATGCAT 5 cut(s) 345, 1135, 1273, 1509, 2897
NspI RCATGY 2 cut(s) 1374, 2231
PagI TCATGA 2 cut(s) 448, 909
PceI AGGCCT 1 cut(s) 825
PciI ACATGT 1 cut(s) 2227
PciSI GCTCTTC 2 cut(s) 651, 1124
PctI GAATGC 1 cut(s) 1133
PdmI GAANNNNTTC 5 cut(s) 505, 1203, 1356, 1697, 2736
PfeI GAWTC 3 cut(s) 353, 539, 2692
Pfl23II CGTACG 1 cut(s) 685
PfoI TCCNGGA 1 cut(s) 2770
PkrI GCNGC 4 cut(s) 1855, 2240, 2450, 3080
PleI GAGTC 9 cut(s) 271, 643, 853, 1285, 1558, 1782, 1921, 2509, 3092
PmaCI CACGTG 1 cut(s) 1260
PmlI CACGTG 1 cut(s) 1260
PpsI GAGTC 9 cut(s) 271, 643, 853, 1285, 1558, 1782, 1921, 2509, 3092
Ppu21I YACGTR 1 cut(s) 1260
PscI ACATGT 1 cut(s) 2227
PshBI ATTAAT 1 cut(s) 2184
PsiI TTATAA 2 cut(s) 750, 1730
Psp124BI GAGCTC 1 cut(s) 1119
Psp1406I AACGTT 1 cut(s) 2018
Psp6I CCWGG 4 cut(s) 1561, 2603, 2770, 3239
PspCI CACGTG 1 cut(s) 1260
PspEI GGTNACC 1 cut(s) 2982
PspFI CCCAGC 1 cut(s) 2841
PspGI CCWGG 4 cut(s) 1561, 2603, 2770, 3239
PspLI CGTACG 1 cut(s) 685
PspN4I GGNNCC 4 cut(s) 19, 55, 3064, 3065
PspOMI GGGCCC 1 cut(s) 3062
PspPI GGNCC 9 cut(s) 18, 973, 1468, 2672, 2925, 3062, 3063, 3192, 3237
PsuI RGATCY 7 cut(s) 394, 570, 1291, 1932, 2128, 2272, 3307
RseI CAYNNNNRTG 4 cut(s) 310, 348, 1888, 3325
SacI GAGCTC 1 cut(s) 1119
SapI GCTCTTC 2 cut(s) 651, 1124
SatI GCNGC 4 cut(s) 1854, 2239, 2449, 3079
Sau96I GGNCC 9 cut(s) 18, 973, 1468, 2672, 2925, 3062, 3063, 3192, 3237
ScaI AGTACT 1 cut(s) 1455
SchI GAGTC 9 cut(s) 272, 644, 853, 1286, 1559, 1782, 1921, 2510, 3093
ScrFI CCNGG 4 cut(s) 1563, 2605, 2772, 3241
SduI GDGCHC 5 cut(s) 1119, 1314, 1617, 2815, 3066
SfaNI GCATC 9 cut(s) 915, 1258, 1318, 1494, 2428, 2904, 3060, 3213, 3334
SfcI CTRYAG 3 cut(s) 836, 1961, 3227
SinI GGWCC 3 cut(s) 973, 2925, 3192
SmiMI CAYNNNNRTG 4 cut(s) 310, 348, 1888, 3325
SmlI CTYRAG 3 cut(s) 1859, 1901, 3152
SmoI CTYRAG 3 cut(s) 1859, 1901, 3152
SseBI AGGCCT 1 cut(s) 825
SsiI CCGC 5 cut(s) 2388, 2417, 2675, 3028, 3081
SspI AATATT 1 cut(s) 1820
SspMI CTAG 6 cut(s) 1211, 1517, 1842, 2034, 2261, 2435
SstI GAGCTC 1 cut(s) 1119
StuI AGGCCT 1 cut(s) 825
StyD4I CCNGG 4 cut(s) 1561, 2603, 2770, 3239
StyI CCWWGG 2 cut(s) 1841, 1889
TaaI ACNGT 6 cut(s) 745, 1103, 1715, 2547, 2821, 2888
TaqI TCGA 7 cut(s) 356, 1343, 1989, 2333, 2724, 2949, 3134
TatI WGTACW 3 cut(s) 1250, 1453, 1897
TfiI GAWTC 3 cut(s) 353, 539, 2692
TscAI CASTG 4 cut(s) 1108, 1627, 1879, 2905
TseFI GTSAC 7 cut(s) 680, 1097, 1424, 1601, 2564, 2869, 2982
TseI GCWGC 4 cut(s) 1853, 2238, 2448, 3078
Tsp45I GTSAC 7 cut(s) 680, 1097, 1424, 1601, 2564, 2869, 2982
TspGWI ACGGA 3 cut(s) 543, 2917, 2986
TspRI CASTG 4 cut(s) 1108, 1627, 1879, 2905
Vha464I CTTAAG 1 cut(s) 1901
VneI GTGCAC 1 cut(s) 1613
VpaK11BI GGWCC 3 cut(s) 973, 2925, 3192
VspI ATTAAT 1 cut(s) 2184
XagI CCTNNNNNAGG 1 cut(s) 2119
XbaI TCTAGA 2 cut(s) 2033, 2260
XceI RCATGY 2 cut(s) 1374, 2231
XmaJI CCTAGG 1 cut(s) 1841
XmiI GTMKAC 2 cut(s) 372, 2907
XmnI GAANNNNTTC 5 cut(s) 505, 1203, 1356, 1697, 2736
XspI CTAG 6 cut(s) 1211, 1517, 1842, 2034, 2261, 2435
ZrmI AGTACT 1 cut(s) 1455
Zsp2I ATGCAT 5 cut(s) 345, 1135, 1273, 1509, 2897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.