Rw6G008370

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000397
Physical Location & Seq
Forward (+)
14253951 .. 14254895
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008370.1

Sequence Viewer

Length: 945 bp
ATGAGCGATGAACTAGCCCATGATTTCTTTCCCATCATCAGAGTCTACAAAGACGGTCGAGTCGACCGACTCTGGGGCACATCCACACTCCCTGCATCCATCGATCCCCAAACCGGTGTTCAATCCAAAGACGTCGTCATCTCAACCGCACCACCCGTCTCTGTAAGGCTTTACATTCCCAAATCAACCACAACCGAGTCAACCCGGAAGCTCCCTCTTCTGGTTTACTTTCACGGCGGTGGCTTCTGCAATGGAAGTGCATTCTCTCCCACTTATCACAACTACCTCAACTCTTTAGTCTCTGAAGCCAATGTTGTAGCTGTTTCTGTTGACTATAGGTTGGTGCCGGAGCACCCTCTTCCGGCTGCCTACGAAGATTCATGGGCCGCTCTCAAATGGGTGGAGTCCCATTTCGAAGGAAACGGCCCCGAAGACTGGCTGAACCGGCTTGCAGATTTGAACCGGGTTTTCTTTTCAGGTGATAGCGCTGGGGCTAACATAGCTCACCAAATGGGTTTGAGAGTTGGGTGTGAGAAGTTGGTGGGTTTTAAGCTGAATGGGATTGTTCTGGTGCATCCATACTTTTGGGGTGAAGAACCAGTAGGGGGAGAGCTAGCTTTGGCTGAGAATCATACAAAGATGTTGGCTGCTTTGTGGAGGTTTTGTTACCCTTTGACTAGTGGATCCGATGACCCGGTTCTCAATCCGGGTAAGGATCCGAAGTTGGGAGAGTTGGGTTGTGAGAAAGTGTTGGTCTGCGTTGCGGAGAAGGATTCGTTGAAAGATAGGGGGTGGTATTACAGTGAGGTGTTGAAGAAGAGTGGATGGAATGGAGTAGTGGAGGTTATGGAAGCAAAGGAGGAGCAGCATGTGTTCCATTTGGTCAATCCTAGTTGTGACAATGCTGTGACCATGCTCAAGAAGTTAGTTTCTTTCATAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

314

Amino Acids

34.76

Weight (kDa)

5.59

Isoelectric Point (pI)

28.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 57 - 170 3.5e-09 Carboxylesterase family
BD-FAE PF20434 66 - 176 6.8e-13 BD-FAE
Abhydrolase_3 PF07859 74 - 293 7.1e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 343
AccBSI CCGCTC 1 cut(s) 389
AccI GTMKAC 2 cut(s) 45, 63
AciI CCGC 4 cut(s) 147, 237, 387, 764
AclWI GGATC 5 cut(s) 98, 678, 691, 710, 723
AcuI CTGAAG 1 cut(s) 324
AcyI GRCGYC 1 cut(s) 132
AfeI AGCGCT 1 cut(s) 487
AfiI CCNNNNNNNGG 7 cut(s) 73, 113, 220, 361, 435, 605, 725
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 4 cut(s) 122, 460, 781, 814
AhlI ACTAGT 1 cut(s) 677
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 6 cut(s) 211, 320, 503, 553, 613, 617
AluI AGCT 6 cut(s) 211, 320, 503, 553, 613, 617
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 2 cut(s) 163, 304
AlwI GGATC 5 cut(s) 98, 678, 691, 710, 723
Aor51HI AGCGCT 1 cut(s) 487
AoxI GGCC 2 cut(s) 384, 424
ApeKI GCWGC 3 cut(s) 365, 647, 865
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 384, 425
AsuC2I CCSGG 4 cut(s) 205, 464, 695, 708
AsuHPI GGTGA 3 cut(s) 491, 497, 602
AsuII TTCGAA 1 cut(s) 414
AsuNHI GCTAGC 1 cut(s) 613
BaeGI GKGCMC 1 cut(s) 80
BamHI GGATCC 2 cut(s) 683, 715
BanI GGYRCC 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 3 cut(s) 352, 634, 877
BccI CCATC 3 cut(s) 41, 107, 819
BceAI ACGGC 2 cut(s) 250, 439
BcnI CCSGG 4 cut(s) 205, 464, 695, 708
BcoDI GTCTC 2 cut(s) 163, 304
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 4 cut(s) 14, 614, 678, 891
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 489
BisI GCNGC 4 cut(s) 366, 387, 648, 866
BlsI GCNGC 4 cut(s) 367, 388, 649, 867
Bme1390I CCNGG 4 cut(s) 205, 464, 695, 708
BmgT120I GGNCC 2 cut(s) 384, 425
BmiI GGNNCC 4 cut(s) 345, 427, 685, 717
BmrFI CCNGG 4 cut(s) 205, 464, 695, 708
BmsI GCATC 2 cut(s) 104, 583
BmtI GCTAGC 1 cut(s) 617
BpiI GAAGAC 1 cut(s) 438
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 4 cut(s) 205, 464, 695, 708
Bsa29I ATCGAT 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 2 cut(s) 649, 679
Bsc4I CCNNNNNNNGG 7 cut(s) 73, 113, 220, 361, 435, 605, 725
Bse118I RCCGGY 2 cut(s) 113, 444
Bse1I ACTGG 2 cut(s) 440, 599
Bse3DI GCAATG 1 cut(s) 256
BseCI ATCGAT 1 cut(s) 102
BseGI GGATG 4 cut(s) 80, 95, 574, 830
BseLI CCNNNNNNNGG 7 cut(s) 73, 113, 220, 361, 435, 605, 725
BseMI GCAATG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 2 cut(s) 440, 599
BseRI GAGGAG 1 cut(s) 875
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 3 cut(s) 352, 634, 877
BseYI CCCAGC 1 cut(s) 488
Bsh1285I CGRYCG 2 cut(s) 58, 67
BshFI GGCC 2 cut(s) 386, 426
BshNI GGYRCC 1 cut(s) 343
BshTI ACCGGT 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 102
BsiEI CGRYCG 2 cut(s) 58, 67
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 8 cut(s) 114, 205, 347, 362, 445, 463, 695, 707
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 7 cut(s) 73, 113, 220, 361, 435, 605, 725
BsmAI GTCTC 2 cut(s) 163, 304
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 391
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 2 cut(s) 386, 426
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 2 cut(s) 80, 354
Bsp143I GATC 3 cut(s) 103, 683, 715
BspACI CCGC 4 cut(s) 147, 237, 387, 764
BspANI GGCC 2 cut(s) 386, 426
BspCNI CTCAG 1 cut(s) 616
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 4 cut(s) 345, 427, 685, 717
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 5 cut(s) 98, 678, 691, 710, 723
BspT104I TTCGAA 1 cut(s) 414
BspT107I GGYRCC 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 256
BsrFI RCCGGY 2 cut(s) 113, 444
BsrI ACTGG 2 cut(s) 440, 599
BssAI RCCGGY 2 cut(s) 113, 444
BssMI GATC 3 cut(s) 103, 683, 715
BssNI GRCGYC 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 56, 803
Bst6I CTCTTC 3 cut(s) 222, 363, 812
BstACI GRCGYC 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 450, 615
BstDEI CTNAG 1 cut(s) 624
BstF5I GGATG 4 cut(s) 80, 95, 574, 830
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 3 cut(s) 106, 686, 718
BstMAI GTCTC 2 cut(s) 163, 304
BstMBI GATC 3 cut(s) 103, 683, 715
BstMCI CGRYCG 2 cut(s) 58, 67
BstMWI GCNNNNNNNGC 2 cut(s) 445, 500
BstNSI RCATGY 1 cut(s) 872
BstSCI CCNGG 4 cut(s) 203, 462, 693, 706
BstSFI CTRYAG 1 cut(s) 334
BstSLI GKGCMC 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 352, 634, 877
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 683, 715
BstXI CCANNNNNNTGG 1 cut(s) 585
BstYI RGATCY 2 cut(s) 683, 715
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 2 cut(s) 386, 426
BsuTUI ATCGAT 1 cut(s) 102
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 4 cut(s) 80, 95, 574, 830
BtsIMutI CAGTG 1 cut(s) 808
Cac8I GCNNGC 2 cut(s) 450, 615
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 2 cut(s) 113, 444
Cfr13I GGNCC 2 cut(s) 384, 425
ClaI ATCGAT 1 cut(s) 102
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 4 cut(s) 20, 381, 869, 913
DdeI CTNAG 1 cut(s) 624
DpnI GATC 3 cut(s) 105, 685, 717
DpnII GATC 3 cut(s) 103, 683, 715
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
Eam1104I CTCTTC 3 cut(s) 222, 363, 812
EarI CTCTTC 3 cut(s) 222, 363, 812
Eco47III AGCGCT 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 4 cut(s) 23, 384, 872, 916
FaqI GGGAC 1 cut(s) 391
FatI CATG 4 cut(s) 19, 380, 868, 912
FblI GTMKAC 2 cut(s) 45, 63
Fnu4HI GCNGC 4 cut(s) 366, 387, 648, 866
FokI GGATG 4 cut(s) 67, 82, 561, 837
Fsp4HI GCNGC 4 cut(s) 366, 387, 648, 866
FspBI CTAG 4 cut(s) 14, 614, 678, 891
GlaI GCGC 1 cut(s) 487
GluI GCNGC 4 cut(s) 366, 387, 648, 866
GsaI CCCAGC 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 2 cut(s) 386, 426
HapII CCGG 8 cut(s) 114, 205, 347, 362, 445, 463, 695, 707
HhaI GCGC 1 cut(s) 488
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 4 cut(s) 23, 384, 872, 916
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 3 cut(s) 64, 201, 331
HindII GTYRAC 3 cut(s) 64, 201, 331
HinfI GANTC 8 cut(s) 42, 60, 69, 197, 377, 404, 628, 773
HpaII CCGG 8 cut(s) 114, 205, 347, 362, 445, 463, 695, 707
HphI GGTGA 3 cut(s) 491, 497, 602
Hpy166II GTNNAC 5 cut(s) 46, 64, 201, 226, 331
Hpy188I TCNGA 4 cut(s) 41, 304, 688, 720
Hpy188III TCNNGA 1 cut(s) 919
Hpy8I GTNNAC 5 cut(s) 46, 64, 201, 226, 331
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 2 cut(s) 410, 763
HpyCH4III ACNGT 2 cut(s) 56, 803
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 5 cut(s) 95, 249, 260, 452, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 500
HpyF3I CTNAG 1 cut(s) 624
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 4 cut(s) 23, 384, 872, 916
HspAI GCGC 1 cut(s) 486
Kzo9I GATC 3 cut(s) 103, 683, 715
LmnI GCTCC 3 cut(s) 216, 349, 862
Lsp1109I GCAGC 3 cut(s) 352, 634, 877
LweI GCATC 2 cut(s) 104, 583
MaeI CTAG 4 cut(s) 14, 614, 678, 891
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 665, 896, 907
MalI GATC 3 cut(s) 105, 685, 717
MbiI CCGCTC 1 cut(s) 389
MboI GATC 3 cut(s) 103, 683, 715
MboII GAAGA 7 cut(s) 209, 350, 386, 443, 605, 826, 829
MflI RGATCY 2 cut(s) 683, 715
MhlI GDGCHC 2 cut(s) 80, 354
MluCI AATT 1 cut(s) 940
MlyI GAGTC 5 cut(s) 51, 63, 69, 206, 413
MnlI CCTC 7 cut(s) 225, 296, 366, 651, 799, 835, 853
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 8 cut(s) 114, 205, 347, 362, 445, 463, 695, 707
MspR9I CCNGG 4 cut(s) 205, 464, 695, 708
Mva1269I GAATGC 1 cut(s) 260
MwoI GCNNNNNNNGC 2 cut(s) 445, 500
NciI CCSGG 4 cut(s) 205, 464, 695, 708
NdeII GATC 3 cut(s) 103, 683, 715
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 4 cut(s) 23, 384, 872, 916
NlaIV GGNNCC 4 cut(s) 345, 427, 685, 717
NmuCI GTSAC 2 cut(s) 896, 907
NspI RCATGY 1 cut(s) 872
NspV TTCGAA 1 cut(s) 414
OliI CACNNNNGTG 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 64
PctI GAATGC 1 cut(s) 260
PfeI GAWTC 3 cut(s) 377, 628, 773
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 4 cut(s) 367, 388, 649, 867
PleI GAGTC 5 cut(s) 50, 63, 68, 205, 412
PpsI GAGTC 5 cut(s) 50, 63, 68, 205, 412
PspFI CCCAGC 1 cut(s) 488
PspN4I GGNNCC 4 cut(s) 345, 427, 685, 717
PspPI GGNCC 2 cut(s) 384, 425
PsuI RGATCY 2 cut(s) 683, 715
PsyI GACNNNGTC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 237
SalI GTCGAC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 4 cut(s) 366, 387, 648, 866
Sau3AI GATC 3 cut(s) 103, 683, 715
Sau96I GGNCC 2 cut(s) 384, 425
SchI GAGTC 5 cut(s) 51, 63, 69, 206, 413
ScrFI CCNGG 4 cut(s) 205, 464, 695, 708
SduI GDGCHC 2 cut(s) 80, 354
SfaNI GCATC 2 cut(s) 104, 583
SfcI CTRYAG 1 cut(s) 334
SfuI TTCGAA 1 cut(s) 414
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SpeI ACTAGT 1 cut(s) 677
Sse9I AATT 1 cut(s) 940
SsiI CCGC 4 cut(s) 147, 237, 387, 764
SspMI CTAG 4 cut(s) 14, 614, 678, 891
StyD4I CCNGG 4 cut(s) 203, 462, 693, 706
TaaI ACNGT 2 cut(s) 56, 803
TaiI ACGT 1 cut(s) 135
TaqI TCGA 4 cut(s) 58, 63, 102, 414
TaqII GACCGA 1 cut(s) 81
TasI AATT 1 cut(s) 940
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 3 cut(s) 377, 628, 773
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 1 cut(s) 808
TseFI GTSAC 2 cut(s) 896, 907
TseI GCWGC 3 cut(s) 365, 647, 865
Tsp45I GTSAC 2 cut(s) 896, 907
TspDTI ATGAA 3 cut(s) 24, 369, 925
TspRI CASTG 1 cut(s) 808
Tth111I GACNNNGTC 1 cut(s) 134
XceI RCATGY 1 cut(s) 872
XmiI GTMKAC 2 cut(s) 45, 63
XspI CTAG 4 cut(s) 14, 614, 678, 891
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.