Rroxscaffold_178G00437320

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000178
OG0000397
Physical Location & Seq
Forward (+)
119836 .. 120789
954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_178G00437320.1

Sequence Viewer

Length: 954 bp
ATGAGCGACGAACTAGCCCATGATTTCTCTCCCATGATCAAAGTATACAAAGACGGTCGAATCGACCGACTCCTAGGCACATCCACAGTCCCTGCATCCATTGATCCCCAAACCGGTGTTCAATCCAAAGACGTCGTCATCTCAACCGCACCACCCATCTCTGCAAGGCTTTACATTCCCAAATCCACCGCAACCGAGTCAGCCGAGTCAACCCAGAAGCTCCCTCTTCTGGTTTACTTTCACGGCGGTGGCTTCTGCATTGAAAGTGCTTTCTCTCCCACTTATCACAACTACCTCAACTCTTTAGTCTCCGAGGCCAATGTTGCAGCTGTTTCTGTTGAATATAGGCTGGCGCCGGAGCATCCTCTCCCGGCTGCCTATGAAGATTCGTGGGCCGCACTCAAATGGGTGGAGTCCCATCTCGCCGGAAACGGCCCGGAAGACTGGCTGAACCGGTATGCAGACTTGAACCGGGTTTTCTTTTCGGGTGATAGCGCTGGGGCTAACATAGCTCACCACATGGGTTTGAGAGTTGGATCTGAGAAGCTGGTGGGTTTTAAGCTGAATGGGATTGTTCTGGTGCATCCCTACTTTTGGGGTGAAGAACCAATAGGGGGAGAAGTAGCTTTGGCCGAGAGTCTTAGAAAGATGATGCCTGCTTTGTGGCAGTTTACTTACCCTTTGACTAGTGGAACCGATGACCCGTTTTTTAATCCGGGTAAGGATCCGAAGTTGGGGGAGTTGGGTTGTGAGAAAGTTTTGGTCTGTGTTGCGGAGAAGGATGCGTTGAAAGATAGGGGGTGGTATTACAGTGAGGTGTTGAAGAAGAGTGGATGGAATGGAGTCGTGGAGGTATCAGAAGCGAAGGAGGAGCAGCATGTGTTCCATTTGTTCAATGGTACTTGTGACAATGCTGTGACCATGCTCAAGAAGATCGTTTCTTTCATGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

317

Amino Acids

34.88

Weight (kDa)

5.39

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 58 - 180 1.4e-08 Carboxylesterase family
BD-FAE PF20434 68 - 182 1.2e-12 BD-FAE
Abhydrolase_3 PF07859 77 - 296 2.7e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 352
AccI GTMKAC 1 cut(s) 45
AciI CCGC 5 cut(s) 147, 189, 246, 396, 773
AclWI GGATC 4 cut(s) 98, 544, 719, 732
AcoI YGGCCR 1 cut(s) 630
AcyI GRCGYC 2 cut(s) 132, 353
AfaI GTAC 1 cut(s) 901
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 5 cut(s) 113, 229, 594, 614, 734
AgeI ACCGGT 2 cut(s) 113, 453
AgsI TTSAA 7 cut(s) 122, 263, 341, 469, 790, 823, 895
AhlI ACTAGT 1 cut(s) 686
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 246
AluBI AGCT 6 cut(s) 220, 329, 512, 547, 562, 626
AluI AGCT 6 cut(s) 220, 329, 512, 547, 562, 626
Alw26I GTCTC 1 cut(s) 313
AlwI GGATC 4 cut(s) 98, 544, 719, 732
AlwNI CAGNNNCTG 1 cut(s) 92
Aor51HI AGCGCT 1 cut(s) 496
AoxI GGCC 4 cut(s) 315, 393, 433, 630
ApeKI GCWGC 3 cut(s) 326, 374, 874
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 2 cut(s) 113, 453
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 2 cut(s) 355, 497
AspS9I GGNCC 2 cut(s) 393, 434
AsuC2I CCSGG 4 cut(s) 371, 437, 473, 717
AsuHPI GGTGA 3 cut(s) 500, 506, 611
AvrII CCTAGG 1 cut(s) 73
BamHI GGATCC 1 cut(s) 724
BanI GGYRCC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 447
BbvI GCAGC 3 cut(s) 338, 361, 886
BccI CCATC 3 cut(s) 164, 426, 828
BceAI ACGGC 2 cut(s) 259, 448
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 4 cut(s) 371, 437, 473, 717
BcoDI GTCTC 1 cut(s) 313
BcuI ACTAGT 1 cut(s) 686
BfaI CTAG 3 cut(s) 14, 74, 687
BfoI RGCGCY 2 cut(s) 356, 498
BisI GCNGC 4 cut(s) 327, 375, 396, 875
BlnI CCTAGG 1 cut(s) 73
BlsI GCNGC 4 cut(s) 328, 376, 397, 876
Bme1390I CCNGG 4 cut(s) 371, 437, 473, 717
BmgT120I GGNCC 2 cut(s) 393, 434
BmiI GGNNCC 3 cut(s) 354, 694, 726
BmrFI CCNGG 4 cut(s) 371, 437, 473, 717
BmsI GCATC 5 cut(s) 104, 370, 592, 642, 772
BpiI GAAGAC 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 911
BpuMI CCSGG 4 cut(s) 371, 437, 473, 717
BsaHI GRCGYC 2 cut(s) 132, 353
BsaJI CCNNGG 2 cut(s) 73, 312
BsaWI WCCGGW 2 cut(s) 113, 453
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 229, 594, 614, 734
Bse118I RCCGGY 2 cut(s) 113, 453
Bse1I ACTGG 1 cut(s) 449
BseDI CCNNGG 2 cut(s) 73, 312
BseGI GGATG 6 cut(s) 80, 95, 361, 583, 787, 839
BseLI CCNNNNNNNGG 5 cut(s) 113, 229, 594, 614, 734
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 1 cut(s) 449
BseRI GAGGAG 1 cut(s) 884
BseXI GCAGC 3 cut(s) 338, 361, 886
BseYI CCCAGC 1 cut(s) 497
Bsh1285I CGRYCG 2 cut(s) 58, 67
BshFI GGCC 4 cut(s) 317, 395, 435, 632
BshNI GGYRCC 1 cut(s) 352
BshTI ACCGGT 2 cut(s) 113, 453
BsiEI CGRYCG 2 cut(s) 58, 67
BsiSI CCGG 8 cut(s) 114, 356, 371, 426, 437, 454, 472, 716
BslFI GGGAC 2 cut(s) 74, 400
BslI CCNNNNNNNGG 5 cut(s) 113, 229, 594, 614, 734
BsmAI GTCTC 1 cut(s) 313
BsmFI GGGAC 2 cut(s) 74, 400
BsnI GGCC 4 cut(s) 317, 395, 435, 632
Bsp143I GATC 5 cut(s) 36, 103, 536, 724, 933
BspACI CCGC 5 cut(s) 147, 189, 246, 396, 773
BspANI GGCC 4 cut(s) 317, 395, 435, 632
BspCNI CTCAG 1 cut(s) 532
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 3 cut(s) 354, 694, 726
BspPI GGATC 4 cut(s) 98, 544, 719, 732
BspT107I GGYRCC 1 cut(s) 352
BsrFI RCCGGY 2 cut(s) 113, 453
BsrI ACTGG 1 cut(s) 449
BssAI RCCGGY 2 cut(s) 113, 453
BssECI CCNNGG 2 cut(s) 73, 312
BssMI GATC 5 cut(s) 36, 103, 536, 724, 933
BssNAI GTATAC 1 cut(s) 46
BssNI GRCGYC 2 cut(s) 132, 353
BssT1I CCWWGG 1 cut(s) 73
Bst1107I GTATAC 1 cut(s) 46
Bst4CI ACNGT 3 cut(s) 56, 88, 812
Bst6I CTCTTC 2 cut(s) 231, 821
BstACI GRCGYC 2 cut(s) 132, 353
BstC8I GCNNGC 2 cut(s) 351, 657
BstDEI CTNAG 2 cut(s) 540, 641
BstF5I GGATG 6 cut(s) 80, 95, 361, 583, 787, 839
BstH2I RGCGCY 2 cut(s) 356, 498
BstHHI GCGC 2 cut(s) 355, 497
BstKTI GATC 5 cut(s) 39, 106, 539, 727, 936
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 5 cut(s) 36, 103, 536, 724, 933
BstMCI CGRYCG 2 cut(s) 58, 67
BstMWI GCNNNNNNNGC 2 cut(s) 323, 509
BstNSI RCATGY 1 cut(s) 881
BstSCI CCNGG 4 cut(s) 369, 435, 471, 715
BstV1I GCAGC 3 cut(s) 338, 361, 886
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 2 cut(s) 536, 724
BstYI RGATCY 2 cut(s) 536, 724
BstZ17I GTATAC 1 cut(s) 46
BsuRI GGCC 4 cut(s) 317, 395, 435, 632
BtsCI GGATG 6 cut(s) 80, 95, 361, 583, 787, 839
BtsIMutI CAGTG 1 cut(s) 817
Cac8I GCNNGC 2 cut(s) 351, 657
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 945
CfoI GCGC 2 cut(s) 355, 497
Cfr10I RCCGGY 2 cut(s) 113, 453
Cfr13I GGNCC 2 cut(s) 393, 434
Csp6I GTAC 1 cut(s) 900
CspAI ACCGGT 2 cut(s) 113, 453
CviAII CATG 6 cut(s) 20, 34, 520, 878, 922, 946
CviQI GTAC 1 cut(s) 900
DdeI CTNAG 2 cut(s) 540, 641
DinI GGCGCC 1 cut(s) 354
DpnI GATC 5 cut(s) 38, 105, 538, 726, 935
DpnII GATC 5 cut(s) 36, 103, 536, 724, 933
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 231, 821
EarI CTCTTC 2 cut(s) 231, 821
Eco130I CCWWGG 1 cut(s) 73
Eco47III AGCGCT 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 354
EheI GGCGCC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 6 cut(s) 23, 37, 523, 881, 925, 949
FaqI GGGAC 2 cut(s) 74, 400
FatI CATG 6 cut(s) 19, 33, 519, 877, 921, 945
FbaI TGATCA 1 cut(s) 36
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 4 cut(s) 327, 375, 396, 875
FokI GGATG 6 cut(s) 67, 82, 348, 570, 794, 846
Fsp4HI GCNGC 4 cut(s) 327, 375, 396, 875
FspBI CTAG 3 cut(s) 14, 74, 687
GlaI GCGC 2 cut(s) 354, 496
GluI GCNGC 4 cut(s) 327, 375, 396, 875
GsaI CCCAGC 1 cut(s) 501
HaeII RGCGCY 2 cut(s) 356, 498
HaeIII GGCC 4 cut(s) 317, 395, 435, 632
HapII CCGG 8 cut(s) 114, 356, 371, 426, 437, 454, 472, 716
HhaI GCGC 2 cut(s) 355, 497
Hin1I GRCGYC 2 cut(s) 132, 353
Hin1II CATG 6 cut(s) 23, 37, 523, 881, 925, 949
Hin6I GCGC 2 cut(s) 353, 495
HinP1I GCGC 2 cut(s) 353, 495
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 8 cut(s) 60, 69, 197, 206, 386, 413, 637, 843
HpaII CCGG 8 cut(s) 114, 356, 371, 426, 437, 454, 472, 716
HphI GGTGA 3 cut(s) 500, 506, 611
Hpy166II GTNNAC 4 cut(s) 46, 210, 235, 672
Hpy188I TCNGA 4 cut(s) 313, 541, 729, 859
Hpy188III TCNNGA 2 cut(s) 928, 946
Hpy8I GTNNAC 4 cut(s) 46, 210, 235, 672
Hpy99I CGWCG 2 cut(s) 11, 137
HpyAV CCTTC 2 cut(s) 772, 859
HpyCH4III ACNGT 3 cut(s) 56, 88, 812
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 95, 164, 258, 326, 461, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 509
HpyF3I CTNAG 2 cut(s) 540, 641
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 2 cut(s) 132, 353
Hsp92II CATG 6 cut(s) 23, 37, 523, 881, 925, 949
HspAI GCGC 2 cut(s) 353, 495
KasI GGCGCC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 5 cut(s) 36, 103, 536, 724, 933
LmnI GCTCC 3 cut(s) 225, 358, 871
Lsp1109I GCAGC 3 cut(s) 338, 361, 886
LweI GCATC 5 cut(s) 104, 370, 592, 642, 772
MaeI CTAG 3 cut(s) 14, 74, 687
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 905, 916
MalI GATC 5 cut(s) 38, 105, 538, 726, 935
MboI GATC 5 cut(s) 36, 103, 536, 724, 933
MboII GAAGA 7 cut(s) 218, 395, 452, 614, 835, 838, 943
MflI RGATCY 2 cut(s) 536, 724
Mly113I GGCGCC 1 cut(s) 353
MlyI GAGTC 6 cut(s) 63, 206, 215, 422, 646, 852
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 7 cut(s) 234, 305, 307, 375, 808, 844, 862
MseI TTAA 2 cut(s) 558, 711
MslI CAYNNNNRTG 2 cut(s) 246, 403
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 8 cut(s) 114, 356, 371, 426, 437, 454, 472, 716
MspR9I CCNGG 4 cut(s) 371, 437, 473, 717
MwoI GCNNNNNNNGC 2 cut(s) 323, 509
NarI GGCGCC 1 cut(s) 353
NciI CCSGG 4 cut(s) 371, 437, 473, 717
NdeII GATC 5 cut(s) 36, 103, 536, 724, 933
NlaIII CATG 6 cut(s) 23, 37, 523, 881, 925, 949
NlaIV GGNNCC 3 cut(s) 354, 694, 726
NmeAIII GCCGAG 2 cut(s) 229, 658
NmuCI GTSAC 2 cut(s) 905, 916
NspI RCATGY 1 cut(s) 881
OliI CACNNNNGTG 1 cut(s) 246
PagI TCATGA 1 cut(s) 945
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 64
PfeI GAWTC 2 cut(s) 60, 386
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 2 cut(s) 113, 453
PkrI GCNGC 4 cut(s) 328, 376, 397, 876
PleI GAGTC 6 cut(s) 63, 205, 214, 421, 645, 851
PluTI GGCGCC 1 cut(s) 356
PpsI GAGTC 6 cut(s) 63, 205, 214, 421, 645, 851
PspFI CCCAGC 1 cut(s) 497
PspN4I GGNNCC 3 cut(s) 354, 694, 726
PspPI GGNCC 2 cut(s) 393, 434
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 2 cut(s) 536, 724
PsyI GACNNNGTC 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 1 cut(s) 901
RsaNI GTAC 1 cut(s) 900
RseI CAYNNNNRTG 2 cut(s) 246, 403
SaqAI TTAA 2 cut(s) 558, 711
SatI GCNGC 4 cut(s) 327, 375, 396, 875
Sau3AI GATC 5 cut(s) 36, 103, 536, 724, 933
Sau96I GGNCC 2 cut(s) 393, 434
SchI GAGTC 6 cut(s) 63, 206, 215, 422, 646, 852
ScrFI CCNGG 4 cut(s) 371, 437, 473, 717
SfaNI GCATC 5 cut(s) 104, 370, 592, 642, 772
SfoI GGCGCC 1 cut(s) 354
SmiMI CAYNNNNRTG 2 cut(s) 246, 403
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
SpeI ACTAGT 1 cut(s) 686
SsiI CCGC 5 cut(s) 147, 189, 246, 396, 773
SspDI GGCGCC 1 cut(s) 352
SspMI CTAG 3 cut(s) 14, 74, 687
StyD4I CCNGG 4 cut(s) 369, 435, 471, 715
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 3 cut(s) 56, 88, 812
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 58, 63
TaqII GACCGA 1 cut(s) 81
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 2 cut(s) 60, 386
Tru1I TTAA 2 cut(s) 558, 711
Tru9I TTAA 2 cut(s) 558, 711
TscAI CASTG 1 cut(s) 817
TseFI GTSAC 2 cut(s) 905, 916
TseI GCWGC 3 cut(s) 326, 374, 874
Tsp45I GTSAC 2 cut(s) 905, 916
TspDTI ATGAA 2 cut(s) 396, 934
TspRI CASTG 1 cut(s) 817
Tth111I GACNNNGTC 1 cut(s) 134
XceI RCATGY 1 cut(s) 881
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XmaJI CCTAGG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 3 cut(s) 14, 74, 687
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.