FvH4_2g06430

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000397
Physical Location & Seq
Forward (+)
5349442 .. 5350904
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06430.t1

Sequence Viewer

Length: 954 bp
ATGAGCGACGAAGTAGCCCATGATTTCTATCCCATCATCAGAGTATACAAAGATGGTCGAATCGACCGACTCTGGGACACATCCCCAGTTCCTGCAACTATTGATCCCCAAACCAGCGTTCAATCCAAAGACGTCGTCATCTCAACCACACCACTCATCTCTGCAAGGCTTTACCTCCCTAAATCCGCCACACCCGAGTCAACCGGCAAGTTCCCTCTTCTTATTTACTTTCACGGCGGTGGCTTCTGCAATGGAAGTGCCTTCTCTCCCACTTATCACAACCACCTCAACTTATTAGTCTCCGAGGCCAATGTTGTTGCTGTGTCTGTGGAGTATAGATTGGTGCCGGAGCACCCTCTTCCGGCTGCCTATGAGGATTCATGGGCTGCTGTAAAATGGGTGGAAACCCATTTCGCCGGAACGGGCCCGGAAGAGTGGCTGAACCAGCATGCAGATTTGAACCGGGTTTTCTTTTCAGGTGATAGTGCAGGGGCTAACATAGCTCACCAAATGGGTTTGAAAGTTGGGACCGAGAAGTTGGTGGGTTTTAAGCTGAATGGGATTGTTCTAGTGCATCCCTACTTTTGGGGTGAAGAACCAATAGGGGGAGAGCTAGCTTTGGCTGAGAATACTAGAAAGATGTTGGCTGATTTGTGGAGGTTTTGTTACCCTTTAACTAGTGGAACCGATGACCCGTTTCTTAATCCGGGTAAGGATCCAAAGTTGGCGGAGCTGGGTTGTGGGAAAGTGTTGGTCTGTGTTGCGGAGAAGGATTCGGTGAAAGATAGAGGGTTGTATTACAGTGAGGTCAGTGAGGTGTTGAAGAAAAGTGGATGGAATGGAGTTGTGGAGGTTATGGAAGCAAAGGAGGAGGATCATGTGTTCCATTTGATCGATAATAGTTGTGACAATGCTAGGACCATGCTCAAGAAGGTTGTTTCTTTCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

318

Amino Acids

35.12

Weight (kDa)

5.37

Isoelectric Point (pI)

28.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 58 - 173 4.8e-08 Carboxylesterase family
BD-FAE PF20434 65 - 179 5e-14 BD-FAE
Abhydrolase_3 PF07859 74 - 296 2.7e-48 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 45
AciI CCGC 4 cut(s) 186, 237, 728, 764
AclWI GGATC 4 cut(s) 98, 710, 723, 882
AcyI GRCGYC 1 cut(s) 132
AfiI CCNNNNNNNGG 4 cut(s) 73, 361, 585, 605
AgsI TTSAA 4 cut(s) 122, 460, 520, 823
AhlI ACTAGT 1 cut(s) 677
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 237
AloI GAACNNNNNNTCC 2 cut(s) 866, 898
AluBI AGCT 5 cut(s) 503, 553, 613, 617, 733
AluI AGCT 5 cut(s) 503, 553, 613, 617, 733
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 4 cut(s) 98, 710, 723, 882
AlwNI CAGNNNCTG 1 cut(s) 92
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 2 cut(s) 306, 424
ApaI GGGCCC 1 cut(s) 428
ApeKI GCWGC 2 cut(s) 365, 386
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AspS9I GGNCC 4 cut(s) 424, 425, 528, 918
AsuC2I CCSGG 3 cut(s) 428, 464, 708
AsuHPI GGTGA 4 cut(s) 491, 497, 602, 790
AsuNHI GCTAGC 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 2 cut(s) 528, 918
BaeGI GKGCMC 1 cut(s) 428
BamHI GGATCC 1 cut(s) 715
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 2 cut(s) 352, 373
BccI CCATC 3 cut(s) 41, 47, 828
BceAI ACGGC 1 cut(s) 250
BcnI CCSGG 3 cut(s) 428, 464, 708
BcoDI GTCTC 1 cut(s) 304
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 5 cut(s) 569, 614, 633, 678, 915
BisI GCNGC 2 cut(s) 366, 387
BlsI GCNGC 2 cut(s) 367, 388
Bme1390I CCNGG 3 cut(s) 428, 464, 708
Bme18I GGWCC 2 cut(s) 528, 918
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 4 cut(s) 424, 425, 528, 918
BmiI GGNNCC 5 cut(s) 345, 426, 529, 685, 717
BmrFI CCNGG 3 cut(s) 428, 464, 708
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 583
BmtI GCTAGC 1 cut(s) 617
BmuI ACTGGG 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 911
BpuMI CCSGG 3 cut(s) 428, 464, 708
Bsa29I ATCGAT 1 cut(s) 894
BsaBI GATNNNNATC 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 649, 679
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 361, 585, 605
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 86
Bse3DI GCAATG 1 cut(s) 256
Bse8I GATNNNNATC 1 cut(s) 27
BseCI ATCGAT 1 cut(s) 894
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 3 cut(s) 80, 574, 839
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 4 cut(s) 73, 361, 585, 605
BseMI GCAATG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 1 cut(s) 86
BseRI GAGGAG 1 cut(s) 884
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 2 cut(s) 352, 373
BseYI CCCAGC 1 cut(s) 733
BsgI GTGCAG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 2 cut(s) 308, 426
BshNI GGYRCC 1 cut(s) 343
BshVI ATCGAT 1 cut(s) 894
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 7 cut(s) 204, 347, 362, 417, 428, 463, 707
BslFI GGGAC 2 cut(s) 89, 541
BslI CCNNNNNNNGG 4 cut(s) 73, 361, 585, 605
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 2 cut(s) 89, 541
BsnI GGCC 2 cut(s) 308, 426
BsoBI CYCGRG 1 cut(s) 194
Bsp120I GGGCCC 1 cut(s) 424
Bsp1286I GDGCHC 2 cut(s) 354, 428
Bsp143I GATC 4 cut(s) 103, 715, 874, 891
BspACI CCGC 4 cut(s) 186, 237, 728, 764
BspANI GGCC 2 cut(s) 308, 426
BspCNI CTCAG 1 cut(s) 616
BspDI ATCGAT 1 cut(s) 894
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 5 cut(s) 345, 426, 529, 685, 717
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 4 cut(s) 98, 710, 723, 882
BspT107I GGYRCC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 4 cut(s) 103, 715, 874, 891
BssNAI GTATAC 1 cut(s) 46
BssNI GRCGYC 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 803
Bst6I CTCTTC 3 cut(s) 222, 363, 426
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 450, 615
BstDEI CTNAG 1 cut(s) 624
BstF5I GGATG 3 cut(s) 80, 574, 839
BstKTI GATC 4 cut(s) 106, 718, 877, 894
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 4 cut(s) 103, 715, 874, 891
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 2 cut(s) 445, 500
BstNSI RCATGY 1 cut(s) 452
BstSCI CCNGG 3 cut(s) 426, 462, 706
BstSLI GKGCMC 1 cut(s) 428
BstV1I GCAGC 2 cut(s) 352, 373
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BstZ17I GTATAC 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 894
BsuRI GGCC 2 cut(s) 308, 426
BsuTUI ATCGAT 1 cut(s) 894
BtsCI GGATG 3 cut(s) 80, 574, 839
BtsIMutI CAGTG 2 cut(s) 808, 817
Cac8I GCNNGC 2 cut(s) 450, 615
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 945
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 4 cut(s) 424, 425, 528, 918
ClaI ATCGAT 1 cut(s) 894
CviAII CATG 6 cut(s) 20, 381, 449, 878, 922, 946
DdeI CTNAG 1 cut(s) 624
DpnI GATC 4 cut(s) 105, 717, 876, 893
DpnII GATC 4 cut(s) 103, 715, 874, 891
Eam1104I CTCTTC 3 cut(s) 222, 363, 426
EarI CTCTTC 3 cut(s) 222, 363, 426
EciI GGCGGA 2 cut(s) 175, 743
Eco24I GRGCYC 1 cut(s) 428
Eco47I GGWCC 2 cut(s) 528, 918
Eco88I CYCGRG 1 cut(s) 194
EcoT38I GRGCYC 1 cut(s) 428
FaeI CATG 6 cut(s) 23, 384, 452, 881, 925, 949
FaqI GGGAC 2 cut(s) 89, 541
FatI CATG 6 cut(s) 19, 380, 448, 877, 921, 945
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 2 cut(s) 366, 387
FokI GGATG 3 cut(s) 67, 561, 846
FriOI GRGCYC 1 cut(s) 428
Fsp4HI GCNGC 2 cut(s) 366, 387
FspBI CTAG 5 cut(s) 569, 614, 633, 678, 915
GluI GCNGC 2 cut(s) 366, 387
GsaI CCCAGC 1 cut(s) 737
HaeIII GGCC 2 cut(s) 308, 426
HapII CCGG 7 cut(s) 204, 347, 362, 417, 428, 463, 707
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 6 cut(s) 23, 384, 452, 881, 925, 949
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HinfI GANTC 5 cut(s) 60, 69, 197, 377, 773
HpaII CCGG 7 cut(s) 204, 347, 362, 417, 428, 463, 707
HphI GGTGA 4 cut(s) 491, 497, 602, 790
Hpy166II GTNNAC 2 cut(s) 46, 201
Hpy188I TCNGA 2 cut(s) 41, 304
Hpy188III TCNNGA 2 cut(s) 928, 946
Hpy8I GTNNAC 2 cut(s) 46, 201
Hpy99I CGWCG 2 cut(s) 11, 137
HpyAV CCTTC 3 cut(s) 271, 763, 925
HpyCH4III ACNGT 1 cut(s) 803
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 95, 164, 249, 452, 488, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 500
HpyF3I CTNAG 1 cut(s) 624
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 6 cut(s) 23, 384, 452, 881, 925, 949
Kzo9I GATC 4 cut(s) 103, 715, 874, 891
LmnI GCTCC 2 cut(s) 349, 730
Lsp1109I GCAGC 2 cut(s) 352, 373
LweI GCATC 1 cut(s) 583
MaeI CTAG 5 cut(s) 569, 614, 633, 678, 915
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 665, 905
MalI GATC 4 cut(s) 105, 717, 876, 893
MboI GATC 4 cut(s) 103, 715, 874, 891
MboII GAAGA 5 cut(s) 209, 350, 443, 605, 835
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 2 cut(s) 354, 428
MluCI AATT 1 cut(s) 949
MlyI GAGTC 2 cut(s) 63, 206
MseI TTAA 3 cut(s) 549, 674, 702
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 7 cut(s) 204, 347, 362, 417, 428, 463, 707
MspR9I CCNGG 3 cut(s) 428, 464, 708
MwoI GCNNNNNNNGC 2 cut(s) 445, 500
NciI CCSGG 3 cut(s) 428, 464, 708
NdeII GATC 4 cut(s) 103, 715, 874, 891
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 6 cut(s) 23, 384, 452, 881, 925, 949
NlaIV GGNNCC 5 cut(s) 345, 426, 529, 685, 717
NmuCI GTSAC 1 cut(s) 905
NspI RCATGY 1 cut(s) 452
OliI CACNNNNGTG 1 cut(s) 237
PaeI GCATGC 1 cut(s) 452
PagI TCATGA 1 cut(s) 945
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PfeI GAWTC 3 cut(s) 60, 377, 773
PflFI GACNNNGTC 1 cut(s) 134
PkrI GCNGC 2 cut(s) 367, 388
PleI GAGTC 2 cut(s) 63, 205
PpsI GAGTC 2 cut(s) 63, 205
PspFI CCCAGC 1 cut(s) 733
PspN4I GGNNCC 5 cut(s) 345, 426, 529, 685, 717
PspOMI GGGCCC 1 cut(s) 424
PspPI GGNCC 4 cut(s) 424, 425, 528, 918
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 1 cut(s) 715
PsyI GACNNNGTC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 3 cut(s) 549, 674, 702
SatI GCNGC 2 cut(s) 366, 387
Sau3AI GATC 4 cut(s) 103, 715, 874, 891
Sau96I GGNCC 4 cut(s) 424, 425, 528, 918
SchI GAGTC 2 cut(s) 63, 206
ScrFI CCNGG 3 cut(s) 428, 464, 708
SduI GDGCHC 2 cut(s) 354, 428
SfaNI GCATC 1 cut(s) 583
SinI GGWCC 2 cut(s) 528, 918
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
SpeI ACTAGT 1 cut(s) 677
SphI GCATGC 1 cut(s) 452
Sse9I AATT 1 cut(s) 949
SsiI CCGC 4 cut(s) 186, 237, 728, 764
SspMI CTAG 5 cut(s) 569, 614, 633, 678, 915
StyD4I CCNGG 3 cut(s) 426, 462, 706
TaaI ACNGT 1 cut(s) 803
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 58, 63, 894
TaqII GACCGA 2 cut(s) 81, 545
TasI AATT 1 cut(s) 949
TfiI GAWTC 3 cut(s) 60, 377, 773
Tru1I TTAA 3 cut(s) 549, 674, 702
Tru9I TTAA 3 cut(s) 549, 674, 702
TscAI CASTG 2 cut(s) 808, 817
TseFI GTSAC 1 cut(s) 905
TseI GCWGC 2 cut(s) 365, 386
Tsp45I GTSAC 1 cut(s) 905
TspDTI ATGAA 2 cut(s) 369, 934
TspRI CASTG 2 cut(s) 808, 817
Tth111I GACNNNGTC 1 cut(s) 134
VpaK11BI GGWCC 2 cut(s) 528, 918
XceI RCATGY 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 5 cut(s) 569, 614, 633, 678, 915
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.