FvH4_2g06400

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000397
Physical Location & Seq
Forward (+)
5320941 .. 5322175
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06400.t1

Sequence Viewer

Length: 615 bp
ATGAGCGACGAAGTAGCCCATGATTTCTATCCCATCATCAAAGTATACAAAGACGGTCGAATCGAAAGACTCTGGGACACATCCCCAGTCCCTGCAACGATTGATCCCCAAACCGGTGTTCAATCCAAAGACGTCGTCATCTCAACCATACCACTCATCTCTGCAAGACTTTACCTCCCTAAATCCGCCACACCCGAGTCAACCGGCAAGTTCCCTCTTCTTGTTTACTTTCACGGCGGTGGCTTCTGCATCGAGAGTGCTTTCTCTCGCACTTATCACAACTACCTCAACTCTTTCGTTTCCGAGGCCAATATTGTAGCTGTTTCTGTTGACTATAGGTTGGCGCCGGAGCACCCTTTGCCGGCAGCATATGAGGACTCATGGGCGGCTCTAAAATGGGTGGAGTCCCATTTCGCCGGAACCGGCCCGGAAGAGTGGCTGAACCAGCATGCAGATCTGAACCGGGTTTTCTTTGCAGGTGATAGTGCAGGGGCTAACATTGCTCACCACATGGGTTTGAGAGTTGGGTCTGAGAAGCCGGTGGGTTTTATGCTGAATGGGATTGTTCTGGTGCATCCCTACTTTGGGGGTGAAGAACCAATAGGGGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

205

Amino Acids

22.45

Weight (kDa)

5.26

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 58 - 171 2.3e-09 Carboxylesterase family
BD-FAE PF20434 65 - 176 1.7e-13 BD-FAE
Abhydrolase_3 PF07859 74 - 194 2.7e-40 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 467
AatII GACGTC 1 cut(s) 135
Acc36I ACCTGC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 45
AciI CCGC 3 cut(s) 186, 237, 386
AclWI GGATC 1 cut(s) 98
AcyI GRCGYC 2 cut(s) 132, 344
AfiI CCNNNNNNNGG 4 cut(s) 113, 361, 584, 585
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 2 cut(s) 320, 612
AluI AGCT 2 cut(s) 320, 612
Alw21I GWGCWC 1 cut(s) 354
AlwI GGATC 1 cut(s) 98
AlwNI CAGNNNCTG 1 cut(s) 92
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 2 cut(s) 306, 424
ApeKI GCWGC 1 cut(s) 365
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 1 cut(s) 425
AsuC2I CCSGG 2 cut(s) 428, 464
AsuHPI GGTGA 3 cut(s) 491, 497, 602
AvaI CYCGRG 1 cut(s) 194
BanI GGYRCC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 377
BccI CCATC 1 cut(s) 41
BceAI ACGGC 1 cut(s) 250
BcnI CCSGG 2 cut(s) 428, 464
BfaI CTAG 1 cut(s) 613
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 347
BfuAI ACCTGC 1 cut(s) 467
BglII AGATCT 1 cut(s) 454
BisI GCNGC 2 cut(s) 366, 387
BlsI GCNGC 2 cut(s) 367, 388
Bme1390I CCNGG 2 cut(s) 428, 464
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 2 cut(s) 345, 421
BmrFI CCNGG 2 cut(s) 428, 464
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 258, 583
BmuI ACTGGG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 428, 464
BsaBI GATNNNNATC 1 cut(s) 27
BsaHI GRCGYC 2 cut(s) 132, 344
BsaJI CCNNGG 1 cut(s) 303
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 2 cut(s) 159, 189
Bsc4I CCNNNNNNNGG 4 cut(s) 113, 361, 584, 585
Bse118I RCCGGY 5 cut(s) 113, 203, 361, 422, 538
Bse1I ACTGG 1 cut(s) 86
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 2 cut(s) 80, 574
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 4 cut(s) 113, 361, 584, 585
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 377
BsgI GTGCAG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 308, 426
BshNI GGYRCC 1 cut(s) 343
BshTI ACCGGT 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 9 cut(s) 114, 204, 347, 362, 417, 423, 428, 463, 539
BslFI GGGAC 3 cut(s) 74, 89, 391
BslI CCNNNNNNNGG 4 cut(s) 113, 361, 584, 585
BsmFI GGGAC 3 cut(s) 74, 89, 391
BsnI GGCC 2 cut(s) 308, 426
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 2 cut(s) 103, 454
BspACI CCGC 3 cut(s) 186, 237, 386
BspANI GGCC 2 cut(s) 308, 426
BspCNI CTCAG 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 345, 421
BspMI ACCTGC 1 cut(s) 467
BspPI GGATC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 5 cut(s) 113, 203, 361, 422, 538
BsrI ACTGG 1 cut(s) 86
BssAI RCCGGY 5 cut(s) 113, 203, 361, 422, 538
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 2 cut(s) 103, 454
BssNAI GTATAC 1 cut(s) 46
BssNI GRCGYC 2 cut(s) 132, 344
Bst1107I GTATAC 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 2 cut(s) 222, 426
BstACI GRCGYC 2 cut(s) 132, 344
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 363, 450
BstDEI CTNAG 1 cut(s) 531
BstF5I GGATG 2 cut(s) 80, 574
BstH2I RGCGCY 1 cut(s) 347
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 2 cut(s) 106, 457
BstMBI GATC 2 cut(s) 103, 454
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 3 cut(s) 358, 445, 500
BstNSI RCATGY 1 cut(s) 452
BstSCI CCNGG 2 cut(s) 426, 462
BstSFI CTRYAG 1 cut(s) 334
BstV1I GCAGC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BstZ17I GTATAC 1 cut(s) 46
BsuRI GGCC 2 cut(s) 308, 426
BtsCI GGATG 2 cut(s) 80, 574
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 2 cut(s) 363, 450
CaiI CAGNNNCTG 1 cut(s) 92
CfoI GCGC 1 cut(s) 346
Cfr10I RCCGGY 5 cut(s) 113, 203, 361, 422, 538
Cfr13I GGNCC 1 cut(s) 425
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 4 cut(s) 20, 381, 449, 511
DdeI CTNAG 1 cut(s) 531
DinI GGCGCC 1 cut(s) 345
DpnI GATC 2 cut(s) 105, 456
DpnII GATC 2 cut(s) 103, 454
Eam1104I CTCTTC 2 cut(s) 222, 426
EarI CTCTTC 2 cut(s) 222, 426
EciI GGCGGA 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 194
EgeI GGCGCC 1 cut(s) 345
EheI GGCGCC 1 cut(s) 345
FaeI CATG 4 cut(s) 23, 384, 452, 514
FaqI GGGAC 3 cut(s) 74, 89, 391
FatI CATG 4 cut(s) 19, 380, 448, 510
FauNDI CATATG 1 cut(s) 370
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 2 cut(s) 366, 387
FokI GGATG 2 cut(s) 67, 561
Fsp4HI GCNGC 2 cut(s) 366, 387
FspBI CTAG 1 cut(s) 613
GlaI GCGC 1 cut(s) 345
GluI GCNGC 2 cut(s) 366, 387
HaeII RGCGCY 1 cut(s) 347
HaeIII GGCC 2 cut(s) 308, 426
HapII CCGG 9 cut(s) 114, 204, 347, 362, 417, 423, 428, 463, 539
HhaI GCGC 1 cut(s) 346
Hin1I GRCGYC 2 cut(s) 132, 344
Hin1II CATG 4 cut(s) 23, 384, 452, 514
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HincII GTYRAC 2 cut(s) 201, 331
HindII GTYRAC 2 cut(s) 201, 331
HinfI GANTC 5 cut(s) 60, 69, 197, 377, 404
HpaII CCGG 9 cut(s) 114, 204, 347, 362, 417, 423, 428, 463, 539
HphI GGTGA 3 cut(s) 491, 497, 602
Hpy166II GTNNAC 4 cut(s) 46, 201, 226, 331
Hpy188I TCNGA 3 cut(s) 304, 459, 532
Hpy188III TCNNGA 1 cut(s) 253
Hpy8I GTNNAC 4 cut(s) 46, 201, 226, 331
Hpy99I CGWCG 2 cut(s) 11, 137
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 7 cut(s) 95, 164, 249, 452, 476, 488, 574
HpyF10VI GCNNNNNNNGC 3 cut(s) 358, 445, 500
HpyF3I CTNAG 1 cut(s) 531
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 2 cut(s) 132, 344
Hsp92II CATG 4 cut(s) 23, 384, 452, 514
HspAI GCGC 1 cut(s) 344
KasI GGCGCC 1 cut(s) 343
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
Kzo9I GATC 2 cut(s) 103, 454
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 1 cut(s) 377
LweI GCATC 2 cut(s) 258, 583
MaeI CTAG 1 cut(s) 613
MaeII ACGT 1 cut(s) 132
MalI GATC 2 cut(s) 105, 456
MboI GATC 2 cut(s) 103, 454
MboII GAAGA 3 cut(s) 209, 443, 605
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 1 cut(s) 354
Mly113I GGCGCC 1 cut(s) 344
MlyI GAGTC 4 cut(s) 63, 206, 371, 413
MnlI CCTC 5 cut(s) 185, 225, 296, 298, 367
MroNI GCCGGC 1 cut(s) 361
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 9 cut(s) 114, 204, 347, 362, 417, 423, 428, 463, 539
MspR9I CCNGG 2 cut(s) 428, 464
MwoI GCNNNNNNNGC 3 cut(s) 358, 445, 500
NaeI GCCGGC 1 cut(s) 363
NarI GGCGCC 1 cut(s) 344
NciI CCSGG 2 cut(s) 428, 464
NdeI CATATG 1 cut(s) 370
NdeII GATC 2 cut(s) 103, 454
NgoMIV GCCGGC 1 cut(s) 361
NlaIII CATG 4 cut(s) 23, 384, 452, 514
NlaIV GGNNCC 2 cut(s) 345, 421
NspI RCATGY 1 cut(s) 452
OliI CACNNNNGTG 1 cut(s) 237
PaeI GCATGC 1 cut(s) 452
PaqCI CACCTGC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PdiI GCCGGC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 60
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 2 cut(s) 367, 388
PleI GAGTC 4 cut(s) 63, 205, 371, 412
PluTI GGCGCC 1 cut(s) 347
PpsI GAGTC 4 cut(s) 63, 205, 371, 412
PspN4I GGNNCC 2 cut(s) 345, 421
PspPI GGNCC 1 cut(s) 425
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 1 cut(s) 454
PsyI GACNNNGTC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 237
SatI GCNGC 2 cut(s) 366, 387
Sau3AI GATC 2 cut(s) 103, 454
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 4 cut(s) 63, 206, 371, 413
ScrFI CCNGG 2 cut(s) 428, 464
SduI GDGCHC 1 cut(s) 354
SetI ASST 7 cut(s) 135, 177, 288, 322, 341, 481, 614
SfaNI GCATC 2 cut(s) 258, 583
SfcI CTRYAG 1 cut(s) 334
SfoI GGCGCC 1 cut(s) 345
SmiMI CAYNNNNRTG 1 cut(s) 237
SphI GCATGC 1 cut(s) 452
SsiI CCGC 3 cut(s) 186, 237, 386
SspDI GGCGCC 1 cut(s) 343
SspI AATATT 1 cut(s) 313
SspMI CTAG 1 cut(s) 613
StyD4I CCNGG 2 cut(s) 426, 462
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 58, 63, 252
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 1 cut(s) 60
TseI GCWGC 1 cut(s) 365
Tth111I GACNNNGTC 1 cut(s) 134
XceI RCATGY 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 1 cut(s) 613
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.