Carboxylesterase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 198 |
| AciI | CCGC | 5 cut(s) | 168, 569, 866, 917, 920 |
| AclWI | GGATC | 7 cut(s) | 281, 705, 718, 866, 890, 903, 1053 |
| AcoI | YGGCCR | 1 cut(s) | 238 |
| AcuI | CTGAAG | 2 cut(s) | 213, 846 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 172, 296, 598, 768, 861, 892, 905 |
| AflIII | ACRYGT | 1 cut(s) | 104 |
| AgeI | ACCGGT | 1 cut(s) | 296 |
| AgsI | TTSAA | 3 cut(s) | 557, 994, 1063 |
| AjiI | CACGTC | 1 cut(s) | 107 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 941, 973 |
| AluBI | AGCT | 5 cut(s) | 394, 465, 736, 830, 988 |
| AluI | AGCT | 5 cut(s) | 394, 465, 736, 830, 988 |
| Alw26I | GTCTC | 1 cut(s) | 113 |
| AlwI | GGATC | 7 cut(s) | 281, 705, 718, 866, 890, 903, 1053 |
| Ama87I | CYCGRG | 1 cut(s) | 886 |
| AoxI | GGCC | 3 cut(s) | 19, 238, 525 |
| AsiGI | ACCGGT | 1 cut(s) | 296 |
| AspLEI | GCGC | 2 cut(s) | 574, 791 |
| AspS9I | GGNCC | 3 cut(s) | 526, 588, 608 |
| AsuC2I | CCSGG | 2 cut(s) | 887, 888 |
| AsuHPI | GGTGA | 2 cut(s) | 432, 674 |
| AvaI | CYCGRG | 1 cut(s) | 886 |
| AvaII | GGWCC | 2 cut(s) | 588, 608 |
| BamHI | GGATCC | 2 cut(s) | 710, 895 |
| BanII | GRGCYC | 1 cut(s) | 342 |
| BauI | CACGAG | 1 cut(s) | 856 |
| BccI | CCATC | 2 cut(s) | 814, 947 |
| BceAI | ACGGC | 1 cut(s) | 253 |
| BciVI | GTATCC | 1 cut(s) | 169 |
| BcnI | CCSGG | 2 cut(s) | 887, 888 |
| BcoDI | GTCTC | 1 cut(s) | 113 |
| BfoI | RGCGCY | 2 cut(s) | 575, 792 |
| BfuI | GTATCC | 1 cut(s) | 169 |
| BglII | AGATCT | 1 cut(s) | 153 |
| BisI | GCNGC | 2 cut(s) | 570, 918 |
| BlsI | GCNGC | 2 cut(s) | 571, 919 |
| Bme1390I | CCNGG | 2 cut(s) | 887, 888 |
| Bme18I | GGWCC | 2 cut(s) | 588, 608 |
| BmeT110I | CYCGRG | 1 cut(s) | 886 |
| BmgBI | CACGTC | 1 cut(s) | 107 |
| BmgT120I | GGNCC | 3 cut(s) | 526, 588, 608 |
| BmiI | GGNNCC | 5 cut(s) | 528, 589, 590, 712, 897 |
| BmrFI | CCNGG | 2 cut(s) | 887, 888 |
| BmsI | GCATC | 1 cut(s) | 766 |
| BpuMI | CCSGG | 2 cut(s) | 887, 888 |
| BsaBI | GATNNNNATC | 1 cut(s) | 180 |
| BsaJI | CCNNGG | 6 cut(s) | 171, 415, 477, 486, 714, 886 |
| BsaWI | WCCGGW | 1 cut(s) | 296 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1000, 1030 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 172, 296, 598, 768, 861, 892, 905 |
| Bse118I | RCCGGY | 1 cut(s) | 296 |
| Bse1I | ACTGG | 1 cut(s) | 782 |
| Bse8I | GATNNNNATC | 1 cut(s) | 180 |
| BseDI | CCNNGG | 6 cut(s) | 171, 415, 477, 486, 714, 886 |
| BseGI | GGATG | 4 cut(s) | 357, 757, 874, 958 |
| BseJI | GATNNNNATC | 1 cut(s) | 180 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 172, 296, 598, 768, 861, 892, 905 |
| BseMII | CTCAG | 3 cut(s) | 68, 339, 936 |
| BseNI | ACTGG | 1 cut(s) | 782 |
| BseRI | GAGGAG | 3 cut(s) | 148, 151, 1055 |
| BseX3I | CGGCCG | 1 cut(s) | 238 |
| Bsh1285I | CGRYCG | 1 cut(s) | 241 |
| BshFI | GGCC | 3 cut(s) | 21, 240, 527 |
| BshTI | ACCGGT | 1 cut(s) | 296 |
| BsiEI | CGRYCG | 1 cut(s) | 241 |
| BsiHKCI | CYCGRG | 1 cut(s) | 886 |
| BsiSI | CCGG | 2 cut(s) | 297, 887 |
| BslFI | GGGAC | 1 cut(s) | 574 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 172, 296, 598, 768, 861, 892, 905 |
| BsmAI | GTCTC | 1 cut(s) | 113 |
| BsmBI | CGTCTC | 1 cut(s) | 113 |
| BsmFI | GGGAC | 1 cut(s) | 574 |
| BsnI | GGCC | 3 cut(s) | 21, 240, 527 |
| BsoBI | CYCGRG | 1 cut(s) | 886 |
| Bsp1286I | GDGCHC | 1 cut(s) | 342 |
| Bsp143I | GATC | 7 cut(s) | 153, 286, 321, 710, 871, 895, 1045 |
| Bsp19I | CCATGG | 2 cut(s) | 171, 415 |
| BspACI | CCGC | 5 cut(s) | 168, 569, 866, 917, 920 |
| BspANI | GGCC | 3 cut(s) | 21, 240, 527 |
| BspCNI | CTCAG | 3 cut(s) | 67, 338, 937 |
| BspLI | GGNNCC | 5 cut(s) | 528, 589, 590, 712, 897 |
| BspPI | GGATC | 7 cut(s) | 281, 705, 718, 866, 890, 903, 1053 |
| BsrFI | RCCGGY | 1 cut(s) | 296 |
| BsrI | ACTGG | 1 cut(s) | 782 |
| BssAI | RCCGGY | 1 cut(s) | 296 |
| BssECI | CCNNGG | 6 cut(s) | 171, 415, 477, 486, 714, 886 |
| BssMI | GATC | 7 cut(s) | 153, 286, 321, 710, 871, 895, 1045 |
| BssNAI | GTATAC | 1 cut(s) | 199 |
| BssSI | CACGAG | 1 cut(s) | 856 |
| BssT1I | CCWWGG | 4 cut(s) | 171, 415, 477, 486 |
| Bst1107I | GTATAC | 1 cut(s) | 199 |
| Bst2BI | CACGAG | 1 cut(s) | 856 |
| Bst4CI | ACNGT | 4 cut(s) | 67, 346, 802, 983 |
| Bst6I | CTCTTC | 1 cut(s) | 97 |
| BstDEI | CTNAG | 4 cut(s) | 54, 325, 945, 1035 |
| BstDSI | CCRYGG | 2 cut(s) | 171, 415 |
| BstEII | GGTNACC | 1 cut(s) | 438 |
| BstF5I | GGATG | 4 cut(s) | 357, 757, 874, 958 |
| BstH2I | RGCGCY | 2 cut(s) | 575, 792 |
| BstHHI | GCGC | 2 cut(s) | 574, 791 |
| BstKTI | GATC | 7 cut(s) | 156, 289, 324, 713, 874, 898, 1048 |
| BstMAI | GTCTC | 1 cut(s) | 113 |
| BstMBI | GATC | 7 cut(s) | 153, 286, 321, 710, 871, 895, 1045 |
| BstMCI | CGRYCG | 1 cut(s) | 241 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 683 |
| BstPI | GGTNACC | 1 cut(s) | 438 |
| BstSCI | CCNGG | 2 cut(s) | 885, 886 |
| BstX2I | RGATCY | 3 cut(s) | 153, 710, 895 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 422, 618 |
| BstYI | RGATCY | 3 cut(s) | 153, 710, 895 |
| BstZ17I | GTATAC | 1 cut(s) | 199 |
| BstZI | CGGCCG | 1 cut(s) | 238 |
| BsuI | GTATCC | 1 cut(s) | 169 |
| BsuRI | GGCC | 3 cut(s) | 21, 240, 527 |
| BtgI | CCRYGG | 2 cut(s) | 171, 415 |
| BtgZI | GCGATG | 1 cut(s) | 833 |
| BtrI | CACGTC | 1 cut(s) | 107 |
| BtsCI | GGATG | 4 cut(s) | 357, 757, 874, 958 |
| BtsIMutI | CAGTG | 4 cut(s) | 63, 189, 342, 988 |
| CfoI | GCGC | 2 cut(s) | 574, 791 |
| Cfr10I | RCCGGY | 1 cut(s) | 296 |
| Cfr13I | GGNCC | 3 cut(s) | 526, 588, 608 |
| Cfr9I | CCCGGG | 1 cut(s) | 886 |
| CseI | GACGC | 1 cut(s) | 323 |
| CspAI | ACCGGT | 1 cut(s) | 296 |
| CviAII | CATG | 6 cut(s) | 28, 172, 416, 564, 694, 1049 |
| DdeI | CTNAG | 4 cut(s) | 54, 325, 945, 1035 |
| DpnI | GATC | 7 cut(s) | 155, 288, 323, 712, 873, 897, 1047 |
| DpnII | GATC | 7 cut(s) | 153, 286, 321, 710, 871, 895, 1045 |
| EaeI | YGGCCR | 1 cut(s) | 238 |
| EagI | CGGCCG | 1 cut(s) | 238 |
| Eam1104I | CTCTTC | 1 cut(s) | 97 |
| EarI | CTCTTC | 1 cut(s) | 97 |
| EciI | GGCGGA | 2 cut(s) | 881, 935 |
| EclXI | CGGCCG | 1 cut(s) | 238 |
| Eco130I | CCWWGG | 4 cut(s) | 171, 415, 477, 486 |
| Eco24I | GRGCYC | 1 cut(s) | 342 |
| Eco47I | GGWCC | 2 cut(s) | 588, 608 |
| Eco52I | CGGCCG | 1 cut(s) | 238 |
| Eco57I | CTGAAG | 2 cut(s) | 213, 846 |
| Eco88I | CYCGRG | 1 cut(s) | 886 |
| Eco91I | GGTNACC | 1 cut(s) | 438 |
| EcoO109I | RGGNCCY | 2 cut(s) | 526, 588 |
| EcoO65I | GGTNACC | 1 cut(s) | 438 |
| EcoT14I | CCWWGG | 4 cut(s) | 171, 415, 477, 486 |
| EcoT38I | GRGCYC | 1 cut(s) | 342 |
| ErhI | CCWWGG | 4 cut(s) | 171, 415, 477, 486 |
| Esp3I | CGTCTC | 1 cut(s) | 113 |
| FaeI | CATG | 6 cut(s) | 31, 175, 419, 567, 697, 1052 |
| FaqI | GGGAC | 1 cut(s) | 574 |
| FatI | CATG | 6 cut(s) | 27, 171, 415, 563, 693, 1048 |
| FauI | CCCGC | 1 cut(s) | 175 |
| FblI | GTMKAC | 1 cut(s) | 198 |
| Fnu4HI | GCNGC | 2 cut(s) | 570, 918 |
| FokI | GGATG | 4 cut(s) | 344, 744, 881, 965 |
| FriOI | GRGCYC | 1 cut(s) | 342 |
| Fsp4HI | GCNGC | 2 cut(s) | 570, 918 |
| GlaI | GCGC | 2 cut(s) | 573, 790 |
| GluI | GCNGC | 2 cut(s) | 570, 918 |
| HaeII | RGCGCY | 2 cut(s) | 575, 792 |
| HaeIII | GGCC | 3 cut(s) | 21, 240, 527 |
| HapII | CCGG | 2 cut(s) | 297, 887 |
| HgaI | GACGC | 1 cut(s) | 323 |
| HhaI | GCGC | 2 cut(s) | 574, 791 |
| Hin1II | CATG | 6 cut(s) | 31, 175, 419, 567, 697, 1052 |
| Hin6I | GCGC | 2 cut(s) | 572, 789 |
| HinP1I | GCGC | 2 cut(s) | 572, 789 |
| HindIII | AAGCTT | 1 cut(s) | 392 |
| HinfI | GANTC | 5 cut(s) | 243, 351, 560, 794, 1031 |
| HpaII | CCGG | 2 cut(s) | 297, 887 |
| HphI | GGTGA | 2 cut(s) | 432, 674 |
| Hpy166II | GTNNAC | 3 cut(s) | 70, 199, 409 |
| Hpy188I | TCNGA | 8 cut(s) | 57, 328, 715, 778, 852, 900, 1071, 1095 |
| Hpy8I | GTNNAC | 3 cut(s) | 70, 199, 409 |
| HpyAV | CCTTC | 6 cut(s) | 142, 226, 943, 1015, 1093, 1123 |
| HpyCH4III | ACNGT | 4 cut(s) | 67, 346, 802, 983 |
| HpyCH4IV | ACGT | 2 cut(s) | 106, 468 |
| HpyCH4V | TGCA | 2 cut(s) | 757, 964 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 683 |
| HpyF3I | CTNAG | 4 cut(s) | 54, 325, 945, 1035 |
| HpySE526I | ACGT | 2 cut(s) | 106, 468 |
| Hsp92II | CATG | 6 cut(s) | 31, 175, 419, 567, 697, 1052 |
| HspAI | GCGC | 2 cut(s) | 572, 789 |
| KflI | GGGWCCC | 1 cut(s) | 588 |
| Kzo9I | GATC | 7 cut(s) | 153, 286, 321, 710, 871, 895, 1045 |
| LpnPI | CCDG | 8 cut(s) | 310, 321, 543, 624, 657, 795, 900, 917 |
| LweI | GCATC | 1 cut(s) | 766 |
| MaeII | ACGT | 2 cut(s) | 106, 468 |
| MaeIII | GTNAC | 1 cut(s) | 438 |
| MalI | GATC | 7 cut(s) | 155, 288, 323, 712, 873, 897, 1047 |
| MboI | GATC | 7 cut(s) | 153, 286, 321, 710, 871, 895, 1045 |
| MboII | GAAGA | 4 cut(s) | 84, 87, 569, 1006 |
| MfeI | CAATTG | 1 cut(s) | 546 |
| MflI | RGATCY | 3 cut(s) | 153, 710, 895 |
| MhlI | GDGCHC | 1 cut(s) | 342 |
| MluCI | AATT | 4 cut(s) | 372, 546, 705, 902 |
| MlyI | GAGTC | 2 cut(s) | 345, 1040 |
| MmeI | TCCRAC | 3 cut(s) | 299, 706, 875 |
| MseI | TTAA | 1 cut(s) | 738 |
| MslI | CAYNNNNRTG | 2 cut(s) | 420, 981 |
| MspI | CCGG | 2 cut(s) | 297, 887 |
| MspR9I | CCNGG | 2 cut(s) | 887, 888 |
| MunI | CAATTG | 1 cut(s) | 546 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 683 |
| NciI | CCSGG | 2 cut(s) | 887, 888 |
| NcoI | CCATGG | 2 cut(s) | 171, 415 |
| NdeII | GATC | 7 cut(s) | 153, 286, 321, 710, 871, 895, 1045 |
| NlaIII | CATG | 6 cut(s) | 31, 175, 419, 567, 697, 1052 |
| NlaIV | GGNNCC | 5 cut(s) | 528, 589, 590, 712, 897 |
| NmuCI | GTSAC | 1 cut(s) | 438 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 243 |
| PfeI | GAWTC | 3 cut(s) | 243, 560, 794 |
| PinAI | ACCGGT | 1 cut(s) | 296 |
| PkrI | GCNGC | 2 cut(s) | 571, 919 |
| PleI | GAGTC | 2 cut(s) | 345, 1039 |
| PpsI | GAGTC | 2 cut(s) | 345, 1039 |
| PpuMI | RGGWCCY | 1 cut(s) | 588 |
| Psp5II | RGGWCCY | 1 cut(s) | 588 |
| PspEI | GGTNACC | 1 cut(s) | 438 |
| PspN4I | GGNNCC | 5 cut(s) | 528, 589, 590, 712, 897 |
| PspPI | GGNCC | 3 cut(s) | 526, 588, 608 |
| PspPPI | RGGWCCY | 1 cut(s) | 588 |
| PsuI | RGATCY | 3 cut(s) | 153, 710, 895 |
| RseI | CAYNNNNRTG | 2 cut(s) | 420, 981 |
| SaqAI | TTAA | 1 cut(s) | 738 |
| SatI | GCNGC | 2 cut(s) | 570, 918 |
| Sau3AI | GATC | 7 cut(s) | 153, 286, 321, 710, 871, 895, 1045 |
| Sau96I | GGNCC | 3 cut(s) | 526, 588, 608 |
| SchI | GAGTC | 2 cut(s) | 345, 1040 |
| ScrFI | CCNGG | 2 cut(s) | 887, 888 |
| SduI | GDGCHC | 1 cut(s) | 342 |
| SfaNI | GCATC | 1 cut(s) | 766 |
| SinI | GGWCC | 2 cut(s) | 588, 608 |
| SmaI | CCCGGG | 1 cut(s) | 888 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 420, 981 |
| Sse9I | AATT | 4 cut(s) | 372, 546, 705, 902 |
| SsiI | CCGC | 5 cut(s) | 168, 569, 866, 917, 920 |
| StyD4I | CCNGG | 2 cut(s) | 885, 886 |
| StyI | CCWWGG | 4 cut(s) | 171, 415, 477, 486 |
| TaaI | ACNGT | 4 cut(s) | 67, 346, 802, 983 |
| TaiI | ACGT | 2 cut(s) | 109, 471 |
| TaqI | TCGA | 3 cut(s) | 246, 303, 797 |
| TasI | AATT | 4 cut(s) | 372, 546, 705, 902 |
| TauI | GCSGC | 2 cut(s) | 572, 920 |
| TfiI | GAWTC | 3 cut(s) | 243, 560, 794 |
| Tru1I | TTAA | 1 cut(s) | 738 |
| Tru9I | TTAA | 1 cut(s) | 738 |
| TscAI | CASTG | 4 cut(s) | 70, 196, 349, 988 |
| TseFI | GTSAC | 1 cut(s) | 438 |
| Tsp45I | GTSAC | 1 cut(s) | 438 |
| TspDTI | ATGAA | 6 cut(s) | 87, 124, 552, 1046, 1105, 1112 |
| TspGWI | ACGGA | 1 cut(s) | 864 |
| TspMI | CCCGGG | 1 cut(s) | 886 |
| TspRI | CASTG | 4 cut(s) | 70, 196, 349, 988 |
| VpaK11BI | GGWCC | 2 cut(s) | 588, 608 |
| XmaI | CCCGGG | 1 cut(s) | 886 |
| XmiI | GTMKAC | 1 cut(s) | 198 |