MD05G1078900.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000397
Physical Location & Seq
Forward (+)
16622206 .. 16623204
999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1078900.v1.1.491

Sequence Viewer

Length: 999 bp
ATGCCTACAATCTCCATACTCGTATTAATTTTTCTCTTCTCTTTTGTAACGCCGAGCATGAACGATGAAATAGCATATGATTTCTTCCCTTTCATGAGAATATACAAAAATGGTACAATTGAGCGTTTCATGGGTAAACTAAAGGTTCCTCCATCGCTCGATCCCAAAACTGGCGTGCAATCAAAAGACGTCGTGATCTCACATAAGGAAAACATATCTGCGAGGCTTTACCTCCCCAGTTCCACCGCCAACTCAGCCCGCAAGCTCCCTCTTCTTGTCTACTTTCATGGAGGCGGCTTCTGTGTCGAAAGTGCCTTTTCTCCGGTTTATCACAACTACGTCAACTCTTTAGTTGCCATGGCCAATGTGGTTGCTGTCTCTGTTGAATATAGGCTTGCCCCGGAGCACCCTCTGCCTATTGCTTATAAAGATTCATGGACCACTCTCAAATGGGTTGCTTCACATTTTGATGGAAACAGCTCCGAAGAGTGGCTGAAGAGATATGCAGATCCAACGCGTGTGTTTTTCTCCGGCGACAGCGCTGGGGCTAACATAGCACACCACATGGCTTTAAAAGTGGGCACAGAGGGCTTGGCTGGGTTCAAGCTCAATGGGATAGTCTTGGTGCATCCATTTTTCTGGGGGACAGAACTTAGTGGGGCAGAGTTAAAAATGCCACCAATTGTAAGACTATATATGGCCGGCCTGTGGCGTTTTTTGAACCCGTTGAGTAGTGGAACCGATGATCCGCTTTTCAACCCGATTAAGGATCCAAAACTGAGGGACTTGGGGTGTCAGAAGGTGCTGGTTTTTGTTGCTGAGAAAGATTCGTTGTATGATAGGGGAAGGTATTATAGTATGGCACTGAGAAGGAGTGGATGGAAAGGAGTTGTGAAGGTATGGAAAACAAAGGAGGAGAACCATGTATTCCATTTGCTCCAACCCACTAATGAAAAAGCTGTAGCCATGATGAAAAGGATCGTTTTGTTCTTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

333

Amino Acids

37.49

Weight (kDa)

9.35

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 77 - 195 2.2e-10 BD-FAE
COesterase PF00135 77 - 197 1.4e-08 Carboxylesterase family
Abhydrolase_3 PF07859 92 - 311 8.5e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
AatII GACGTC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 279
AccII CGCG 1 cut(s) 517
AciI CCGC 4 cut(s) 246, 259, 294, 749
AclWI GGATC 6 cut(s) 155, 503, 740, 764, 777, 986
AcoI YGGCCR 2 cut(s) 360, 699
AcuI CTGAAG 1 cut(s) 515
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 115
AfeI AGCGCT 1 cut(s) 541
AfiI CCNNNNNNNGG 4 cut(s) 170, 489, 708, 766
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 5 cut(s) 386, 604, 721, 757, 994
AloI GAACNNNNNNTCC 2 cut(s) 730, 762
AluBI AGCT 4 cut(s) 265, 480, 607, 959
AluI AGCT 4 cut(s) 265, 480, 607, 959
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 6 cut(s) 155, 503, 740, 764, 777, 986
Aor51HI AGCGCT 1 cut(s) 541
AoxI GGCC 3 cut(s) 360, 699, 703
AseI ATTAAT 1 cut(s) 26
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 1 cut(s) 438
AsuC2I CCSGG 1 cut(s) 401
AvaII GGWCC 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 584
BalI TGGCCA 1 cut(s) 362
BamHI GGATCC 1 cut(s) 769
Bbv12I GWGCWC 1 cut(s) 408
BccI CCATC 3 cut(s) 160, 464, 873
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 1 cut(s) 382
BfmI CTRYAG 1 cut(s) 960
BfoI RGCGCY 1 cut(s) 543
BisI GCNGC 1 cut(s) 295
BlsI GCNGC 1 cut(s) 296
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 1 cut(s) 438
BmgT120I GGNCC 1 cut(s) 438
BmiI GGNNCC 3 cut(s) 147, 739, 771
BmrFI CCNGG 1 cut(s) 401
BmrI ACTGGG 1 cut(s) 231
BmsI GCATC 1 cut(s) 637
BmuI ACTGGG 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 401
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 357, 399
BsaWI WCCGGW 1 cut(s) 322
Bsc4I CCNNNNNNNGG 4 cut(s) 170, 489, 708, 766
Bse118I RCCGGY 1 cut(s) 701
Bse1I ACTGG 2 cut(s) 175, 237
BseDI CCNNGG 2 cut(s) 357, 399
BseGI GGATG 2 cut(s) 628, 884
BseLI CCNNNNNNNGG 4 cut(s) 170, 489, 708, 766
BseMII CTCAG 4 cut(s) 267, 770, 810, 857
BseNI ACTGG 2 cut(s) 175, 237
BseRI GAGGAG 1 cut(s) 929
BseSI GKGCMC 1 cut(s) 584
BseYI CCCAGC 2 cut(s) 542, 596
Bsh1236I CGCG 1 cut(s) 517
BshFI GGCC 3 cut(s) 362, 701, 705
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 4 cut(s) 323, 401, 531, 702
BslFI GGGAC 2 cut(s) 658, 797
BslI CCNNNNNNNGG 4 cut(s) 170, 489, 708, 766
BsmAI GTCTC 1 cut(s) 382
BsmFI GGGAC 2 cut(s) 658, 797
BsnI GGCC 3 cut(s) 362, 701, 705
Bsp1286I GDGCHC 2 cut(s) 408, 584
Bsp143I GATC 6 cut(s) 160, 195, 508, 745, 769, 978
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 4 cut(s) 246, 259, 294, 749
BspANI GGCC 3 cut(s) 362, 701, 705
BspCNI CTCAG 4 cut(s) 266, 771, 811, 858
BspFNI CGCG 1 cut(s) 517
BspHI TCATGA 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 147, 739, 771
BspPI GGATC 6 cut(s) 155, 503, 740, 764, 777, 986
BsrFI RCCGGY 1 cut(s) 701
BsrI ACTGG 2 cut(s) 175, 237
BssAI RCCGGY 1 cut(s) 701
BssECI CCNNGG 2 cut(s) 357, 399
BssMI GATC 6 cut(s) 160, 195, 508, 745, 769, 978
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 357
Bst6I CTCTTC 4 cut(s) 41, 276, 480, 491
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 412
BstC8I GCNNGC 5 cut(s) 176, 259, 263, 396, 703
BstDEI CTNAG 5 cut(s) 253, 653, 779, 819, 866
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 2 cut(s) 628, 884
BstFNI CGCG 1 cut(s) 517
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 6 cut(s) 163, 198, 511, 748, 772, 981
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 6 cut(s) 160, 195, 508, 745, 769, 978
BstMWI GCNNNNNNNGC 4 cut(s) 254, 412, 554, 588
BstSCI CCNGG 1 cut(s) 399
BstSFI CTRYAG 1 cut(s) 960
BstSLI GKGCMC 1 cut(s) 584
BstUI CGCG 1 cut(s) 517
BstX2I RGATCY 2 cut(s) 508, 769
BstXI CCANNNNNNTGG 1 cut(s) 639
BstYI RGATCY 2 cut(s) 508, 769
BsuRI GGCC 3 cut(s) 362, 701, 705
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 138
BtsCI GGATG 2 cut(s) 628, 884
BtsIMutI CAGTG 1 cut(s) 863
Cac8I GCNNGC 5 cut(s) 176, 259, 263, 396, 703
CciI TCATGA 1 cut(s) 93
CfoI GCGC 1 cut(s) 542
Cfr10I RCCGGY 1 cut(s) 701
Cfr13I GGNCC 1 cut(s) 438
Csp6I GTAC 1 cut(s) 114
CviAII CATG 9 cut(s) 58, 94, 130, 287, 358, 435, 565, 923, 967
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 5 cut(s) 253, 653, 779, 819, 866
DpnI GATC 6 cut(s) 162, 197, 510, 747, 771, 980
DpnII GATC 6 cut(s) 160, 195, 508, 745, 769, 978
DraI TTTAAA 1 cut(s) 573
EaeI YGGCCR 2 cut(s) 360, 699
Eam1104I CTCTTC 4 cut(s) 41, 276, 480, 491
EarI CTCTTC 4 cut(s) 41, 276, 480, 491
Eco130I CCWWGG 1 cut(s) 357
Eco47I GGWCC 1 cut(s) 438
Eco47III AGCGCT 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 515
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 9 cut(s) 61, 97, 133, 290, 361, 438, 568, 926, 970
FaqI GGGAC 2 cut(s) 658, 797
FatI CATG 9 cut(s) 57, 93, 129, 286, 357, 434, 564, 922, 966
FauI CCCGC 1 cut(s) 266
FauNDI CATATG 1 cut(s) 76
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 1 cut(s) 295
FokI GGATG 2 cut(s) 615, 891
FseI GGCCGGCC 1 cut(s) 705
Fsp4HI GCNGC 1 cut(s) 295
GlaI GCGC 1 cut(s) 541
GluI GCNGC 1 cut(s) 295
GsaI CCCAGC 2 cut(s) 546, 600
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 3 cut(s) 362, 701, 705
HapII CCGG 4 cut(s) 323, 401, 531, 702
HhaI GCGC 1 cut(s) 542
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 9 cut(s) 61, 97, 133, 290, 361, 438, 568, 926, 970
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 2 cut(s) 431, 827
HpaII CCGG 4 cut(s) 323, 401, 531, 702
Hpy166II GTNNAC 3 cut(s) 137, 280, 343
Hpy188I TCNGA 2 cut(s) 484, 798
Hpy188III TCNNGA 2 cut(s) 94, 193
Hpy8I GTNNAC 3 cut(s) 137, 280, 343
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 4 cut(s) 793, 840, 864, 889
HpyCH4IV ACGT 2 cut(s) 189, 339
HpyCH4V TGCA 3 cut(s) 178, 506, 628
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 412, 554, 588
HpyF3I CTNAG 5 cut(s) 253, 653, 779, 819, 866
HpySE526I ACGT 2 cut(s) 189, 339
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 9 cut(s) 61, 97, 133, 290, 361, 438, 568, 926, 970
HspAI GCGC 1 cut(s) 540
KroI GCCGGC 1 cut(s) 701
KroNI GCCGGC 1 cut(s) 703
Kzo9I GATC 6 cut(s) 160, 195, 508, 745, 769, 978
LmnI GCTCC 4 cut(s) 270, 403, 485, 942
LweI GCATC 1 cut(s) 637
MaeII ACGT 2 cut(s) 189, 339
MaeIII GTNAC 1 cut(s) 46
MalI GATC 6 cut(s) 162, 197, 510, 747, 771, 980
MboI GATC 6 cut(s) 160, 195, 508, 745, 769, 978
MboII GAAGA 6 cut(s) 28, 76, 263, 497, 508, 982
MfeI CAATTG 2 cut(s) 117, 681
MflI RGATCY 2 cut(s) 508, 769
MhlI GDGCHC 2 cut(s) 408, 584
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 4 cut(s) 27, 117, 681, 994
MluI ACGCGT 1 cut(s) 515
MluNI TGGCCA 1 cut(s) 362
MmeI TCCRAC 2 cut(s) 536, 964
MnlI CCTC 9 cut(s) 159, 216, 242, 279, 284, 420, 580, 774, 907
Mox20I TGGCCA 1 cut(s) 362
MroNI GCCGGC 1 cut(s) 701
MscI TGGCCA 1 cut(s) 362
MseI TTAA 4 cut(s) 26, 572, 668, 765
MslI CAYNNNNRTG 1 cut(s) 468
Msp20I TGGCCA 1 cut(s) 362
MspI CCGG 4 cut(s) 323, 401, 531, 702
MspR9I CCNGG 1 cut(s) 401
MunI CAATTG 2 cut(s) 117, 681
MvnI CGCG 1 cut(s) 517
MwoI GCNNNNNNNGC 4 cut(s) 254, 412, 554, 588
NaeI GCCGGC 1 cut(s) 703
NciI CCSGG 1 cut(s) 401
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 76
NdeII GATC 6 cut(s) 160, 195, 508, 745, 769, 978
NgoMIV GCCGGC 1 cut(s) 701
NlaIII CATG 9 cut(s) 61, 97, 133, 290, 361, 438, 568, 926, 970
NlaIV GGNNCC 3 cut(s) 147, 739, 771
NmeAIII GCCGAG 1 cut(s) 78
PagI TCATGA 1 cut(s) 93
PdiI GCCGGC 1 cut(s) 703
PfeI GAWTC 2 cut(s) 431, 827
PkrI GCNGC 1 cut(s) 296
PshBI ATTAAT 1 cut(s) 26
PsiI TTATAA 1 cut(s) 426
PspFI CCCAGC 2 cut(s) 542, 596
PspN4I GGNNCC 3 cut(s) 147, 739, 771
PspPI GGNCC 1 cut(s) 438
PsuI RGATCY 2 cut(s) 508, 769
RigI GGCCGGCC 1 cut(s) 705
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
RseI CAYNNNNRTG 1 cut(s) 468
SaqAI TTAA 4 cut(s) 26, 572, 668, 765
SatI GCNGC 1 cut(s) 295
Sau3AI GATC 6 cut(s) 160, 195, 508, 745, 769, 978
Sau96I GGNCC 1 cut(s) 438
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 2 cut(s) 408, 584
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 1 cut(s) 960
SinI GGWCC 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 468
Sse9I AATT 4 cut(s) 27, 117, 681, 994
SsiI CCGC 4 cut(s) 246, 259, 294, 749
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 1 cut(s) 357
TaiI ACGT 2 cut(s) 192, 342
TaqI TCGA 2 cut(s) 159, 306
TasI AATT 4 cut(s) 27, 117, 681, 994
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 2 cut(s) 431, 827
Tru1I TTAA 4 cut(s) 26, 572, 668, 765
Tru9I TTAA 4 cut(s) 26, 572, 668, 765
TscAI CASTG 1 cut(s) 870
TspDTI ATGAA 8 cut(s) 74, 81, 82, 118, 275, 423, 966, 986
TspRI CASTG 1 cut(s) 870
VpaK11BI GGWCC 1 cut(s) 438
VspI ATTAAT 1 cut(s) 26
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 279
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.