RLG00000014635

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000397
Physical Location & Seq
Reverse (-)
55582239 .. 55583191
953 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014635

Sequence Viewer

Length: 867 bp
ATGAGCGACGAACTAGCCCATGATTTCTTTCCCATCATCAAAGTATATAAAGACGGTCGAGTCAACCGACTCTGGGGCACATCCACACTCCCTGCATCCTTCGATCCCCAAACCGGTGTTCAATCCAAAGACGTCATCATCTCAACCGCACCACCCGTCTCTGTGAGGCTTTACATTCCCAAATCCACCACAACCGAGTCAGCCGAGTCAACCCAGAAGCTCCCTCTTCTGGTTTACTTTCACGGCGGTGGCTTCTGCAATGGAAGTGCTTTCTCTCCCACTTATCACAACTACCACAACTCTTTAGTCTCTGAAGCCAATGTCGTAGCTGTTTCTGTTGACTATAGGTTGGTGCCGGAGCACCCTCTTCCGGCTGCCTATGAAGATTCATGGGCCACTCTCAAATGGGTGGAGTCCCATTTCGCCGGAAACGGCCCGGAAGACTGGCTGAACCGGCATGCAGACTTGAACCAGCTGAATGGGATTGTTCTGGTGCATCCATACTTTTGGGGTGAAGAACCATTAGGGGGAGAGCTAGCTATAGCTGAGAGTCATAGAAAGATGTTGGCTGCTTTGTGGAGGTTTTGTTACCCTTTGACTAGGGGATCCGATGACCCGGTTCTCAATCCGGGTAAGGATCCGAAGTTGGGGGAGTTGGGTTGTGAGAAAGTGTTGGTCTGTGTTGCGGAGAAGGATTCTTTGAAAGATAGGGGGTGGTATTACAGTGAGATGTGGAAGAAGAGTGGATGGAATGGAGTAGTGGAGGTTTTGGAAGCAAAGGAGGAGCAGCATGTGTTCCATTTGATCAATCCTAGTTGTGAAAATGCTGTGACCATGCTCAAGAAGTTGGTTTCTTTCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

289

Amino Acids

32.25

Weight (kDa)

5.65

Isoelectric Point (pI)

31.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 57 - 117 3.1e-08 Carboxylesterase family
BD-FAE PF20434 68 - 140 3.8e-09 BD-FAE
Abhydrolase_3 PF07859 77 - 155 9.9e-23 alpha/beta hydrolase fold
Abhydrolase_3 PF07859 154 - 267 8.9e-12 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 352
AciI CCGC 3 cut(s) 147, 246, 686
AclWI GGATC 5 cut(s) 98, 600, 613, 632, 645
AcuI CTGAAG 1 cut(s) 333
AcyI GRCGYC 1 cut(s) 132
AfiI CCNNNNNNNGG 6 cut(s) 73, 113, 229, 370, 527, 647
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 3 cut(s) 122, 469, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 246
AluBI AGCT 6 cut(s) 220, 329, 475, 535, 539, 545
AluI AGCT 6 cut(s) 220, 329, 475, 535, 539, 545
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 163, 313
AlwI GGATC 5 cut(s) 98, 600, 613, 632, 645
AoxI GGCC 2 cut(s) 393, 433
ApeKI GCWGC 3 cut(s) 374, 569, 787
AsiGI ACCGGT 1 cut(s) 113
AspS9I GGNCC 2 cut(s) 393, 434
AsuC2I CCSGG 3 cut(s) 437, 617, 630
AsuHPI GGTGA 1 cut(s) 524
AsuNHI GCTAGC 1 cut(s) 535
BaeGI GKGCMC 1 cut(s) 80
BamHI GGATCC 2 cut(s) 605, 637
BanI GGYRCC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 361, 556, 799
BccI CCATC 2 cut(s) 41, 741
BceAI ACGGC 2 cut(s) 259, 448
BclI TGATCA 1 cut(s) 804
BcnI CCSGG 3 cut(s) 437, 617, 630
BcoDI GTCTC 2 cut(s) 163, 313
BfaI CTAG 4 cut(s) 14, 536, 600, 813
BfmI CTRYAG 2 cut(s) 343, 540
BisI GCNGC 3 cut(s) 375, 570, 788
BlsI GCNGC 3 cut(s) 376, 571, 789
Bme1390I CCNGG 3 cut(s) 437, 617, 630
BmgT120I GGNCC 2 cut(s) 393, 434
BmiI GGNNCC 3 cut(s) 354, 607, 639
BmrFI CCNGG 3 cut(s) 437, 617, 630
BmsI GCATC 2 cut(s) 104, 505
BmtI GCTAGC 1 cut(s) 539
BpiI GAAGAC 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 824
BpuMI CCSGG 3 cut(s) 437, 617, 630
BsaHI GRCGYC 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 2 cut(s) 571, 601
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 113, 229, 370, 527, 647
Bse118I RCCGGY 2 cut(s) 113, 453
Bse1I ACTGG 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 265
BseGI GGATG 4 cut(s) 80, 95, 496, 752
BseLI CCNNNNNNNGG 6 cut(s) 73, 113, 229, 370, 527, 647
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 1 cut(s) 449
BseRI GAGGAG 1 cut(s) 797
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 3 cut(s) 361, 556, 799
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 395, 435
BshNI GGYRCC 1 cut(s) 352
BshTI ACCGGT 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 8 cut(s) 114, 356, 371, 426, 437, 454, 617, 629
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 6 cut(s) 73, 113, 229, 370, 527, 647
BsmAI GTCTC 2 cut(s) 163, 313
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 2 cut(s) 395, 435
Bsp1286I GDGCHC 2 cut(s) 80, 363
Bsp143I GATC 4 cut(s) 103, 605, 637, 804
BspACI CCGC 3 cut(s) 147, 246, 686
BspANI GGCC 2 cut(s) 395, 435
BspCNI CTCAG 1 cut(s) 538
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 3 cut(s) 354, 607, 639
BspOI GCTAGC 1 cut(s) 539
BspPI GGATC 5 cut(s) 98, 600, 613, 632, 645
BspT107I GGYRCC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 265
BsrFI RCCGGY 2 cut(s) 113, 453
BsrI ACTGG 1 cut(s) 449
BssAI RCCGGY 2 cut(s) 113, 453
BssMI GATC 4 cut(s) 103, 605, 637, 804
BssNI GRCGYC 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 56, 725
Bst6I CTCTTC 3 cut(s) 231, 372, 734
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 459, 537
BstDEI CTNAG 1 cut(s) 546
BstF5I GGATG 4 cut(s) 80, 95, 496, 752
BstKTI GATC 4 cut(s) 106, 608, 640, 807
BstMAI GTCTC 2 cut(s) 163, 313
BstMBI GATC 4 cut(s) 103, 605, 637, 804
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstNSI RCATGY 2 cut(s) 461, 794
BstSCI CCNGG 3 cut(s) 435, 615, 628
BstSFI CTRYAG 2 cut(s) 343, 540
BstSLI GKGCMC 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 361, 556, 799
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 2 cut(s) 605, 637
BstXI CCANNNNNNTGG 2 cut(s) 479, 507
BstYI RGATCY 2 cut(s) 605, 637
BsuRI GGCC 2 cut(s) 395, 435
BtsCI GGATG 4 cut(s) 80, 95, 496, 752
BtsIMutI CAGTG 1 cut(s) 730
Cac8I GCNNGC 2 cut(s) 459, 537
CciI TCATGA 1 cut(s) 858
Cfr10I RCCGGY 2 cut(s) 113, 453
Cfr13I GGNCC 2 cut(s) 393, 434
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 6 cut(s) 20, 390, 458, 791, 835, 859
DdeI CTNAG 1 cut(s) 546
DpnI GATC 4 cut(s) 105, 607, 639, 806
DpnII GATC 4 cut(s) 103, 605, 637, 804
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
Eam1104I CTCTTC 3 cut(s) 231, 372, 734
EarI CTCTTC 3 cut(s) 231, 372, 734
Eco57I CTGAAG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 6 cut(s) 23, 393, 461, 794, 838, 862
FaqI GGGAC 1 cut(s) 400
FatI CATG 6 cut(s) 19, 389, 457, 790, 834, 858
FbaI TGATCA 1 cut(s) 804
Fnu4HI GCNGC 3 cut(s) 375, 570, 788
FokI GGATG 4 cut(s) 67, 82, 483, 759
Fsp4HI GCNGC 3 cut(s) 375, 570, 788
FspBI CTAG 4 cut(s) 14, 536, 600, 813
GluI GCNGC 3 cut(s) 375, 570, 788
HaeIII GGCC 2 cut(s) 395, 435
HapII CCGG 8 cut(s) 114, 356, 371, 426, 437, 454, 617, 629
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 6 cut(s) 23, 393, 461, 794, 838, 862
HincII GTYRAC 3 cut(s) 64, 210, 340
HindII GTYRAC 3 cut(s) 64, 210, 340
HinfI GANTC 8 cut(s) 60, 69, 197, 206, 386, 413, 550, 695
HpaII CCGG 8 cut(s) 114, 356, 371, 426, 437, 454, 617, 629
HphI GGTGA 1 cut(s) 524
Hpy166II GTNNAC 4 cut(s) 64, 210, 235, 340
Hpy188I TCNGA 3 cut(s) 313, 610, 642
Hpy188III TCNNGA 2 cut(s) 841, 859
Hpy8I GTNNAC 4 cut(s) 64, 210, 235, 340
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 2 cut(s) 109, 685
HpyCH4III ACNGT 2 cut(s) 56, 725
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 4 cut(s) 95, 258, 461, 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpyF3I CTNAG 1 cut(s) 546
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 6 cut(s) 23, 393, 461, 794, 838, 862
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 4 cut(s) 103, 605, 637, 804
LmnI GCTCC 3 cut(s) 225, 358, 784
Lsp1109I GCAGC 3 cut(s) 361, 556, 799
LweI GCATC 2 cut(s) 104, 505
MaeI CTAG 4 cut(s) 14, 536, 600, 813
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 587, 829
MalI GATC 4 cut(s) 105, 607, 639, 806
MboI GATC 4 cut(s) 103, 605, 637, 804
MboII GAAGA 7 cut(s) 218, 359, 395, 452, 527, 748, 751
MflI RGATCY 2 cut(s) 605, 637
MhlI GDGCHC 2 cut(s) 80, 363
MluCI AATT 1 cut(s) 862
MlyI GAGTC 6 cut(s) 63, 69, 206, 215, 422, 559
MnlI CCTC 6 cut(s) 159, 234, 375, 573, 757, 775
MslI CAYNNNNRTG 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 8 cut(s) 114, 356, 371, 426, 437, 454, 617, 629
MspR9I CCNGG 3 cut(s) 437, 617, 630
MwoI GCNNNNNNNGC 1 cut(s) 454
NciI CCSGG 3 cut(s) 437, 617, 630
NdeII GATC 4 cut(s) 103, 605, 637, 804
NheI GCTAGC 1 cut(s) 535
NlaIII CATG 6 cut(s) 23, 393, 461, 794, 838, 862
NlaIV GGNNCC 3 cut(s) 354, 607, 639
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 829
NspI RCATGY 2 cut(s) 461, 794
OliI CACNNNNGTG 1 cut(s) 246
PaeI GCATGC 1 cut(s) 461
PagI TCATGA 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PfeI GAWTC 2 cut(s) 386, 695
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 3 cut(s) 376, 571, 789
PleI GAGTC 6 cut(s) 63, 68, 205, 214, 421, 558
PpsI GAGTC 6 cut(s) 63, 68, 205, 214, 421, 558
PspN4I GGNNCC 3 cut(s) 354, 607, 639
PspPI GGNCC 2 cut(s) 393, 434
PsuI RGATCY 2 cut(s) 605, 637
PvuII CAGCTG 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 246
SatI GCNGC 3 cut(s) 375, 570, 788
Sau3AI GATC 4 cut(s) 103, 605, 637, 804
Sau96I GGNCC 2 cut(s) 393, 434
SchI GAGTC 6 cut(s) 63, 69, 206, 215, 422, 559
ScrFI CCNGG 3 cut(s) 437, 617, 630
SduI GDGCHC 2 cut(s) 80, 363
SfaNI GCATC 2 cut(s) 104, 505
SfcI CTRYAG 2 cut(s) 343, 540
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
SphI GCATGC 1 cut(s) 461
Sse9I AATT 1 cut(s) 862
SsiI CCGC 3 cut(s) 147, 246, 686
SspMI CTAG 4 cut(s) 14, 536, 600, 813
StyD4I CCNGG 3 cut(s) 435, 615, 628
TaaI ACNGT 2 cut(s) 56, 725
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 58, 102
TasI AATT 1 cut(s) 862
TfiI GAWTC 2 cut(s) 386, 695
TscAI CASTG 1 cut(s) 730
TseFI GTSAC 1 cut(s) 829
TseI GCWGC 3 cut(s) 374, 569, 787
Tsp45I GTSAC 1 cut(s) 829
TspDTI ATGAA 3 cut(s) 378, 396, 847
TspRI CASTG 1 cut(s) 730
XceI RCATGY 2 cut(s) 461, 794
XspI CTAG 4 cut(s) 14, 536, 600, 813
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.