Rroxscaffold_7G00205450

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000397
Physical Location & Seq
Forward (+)
55007300 .. 55008243
944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205450.1

Sequence Viewer

Length: 843 bp
ATGAGCGACGAACTAGCCCATGATTTCTCTCCCATGATCAAAGTATACAAAGACGGTCGAATCGACCGACCCTGGGGAACAACCACAGTCCCTGCATCCATCGATCCCCAAACCGGTGTTCAATCCAAAGACGTCGTCATCTCAACCGCACCACCCGTCTCTGTAAGGCTTTACATTCCCAAATCCACCACAACCGAGTCAACCCGGAAGCTCCCTCTTCTGGTTTACTTTCACGGCGGTGGCTTCTGCATTGGAAGTGCTTTCTCTCGCATATATCACAACTACTGCAACTCTTTAGTCTCTGAAGCCAATGTGGTAGCTGTTTCTGTTGACTATAGGTTGGTGCCGGAGCACCCTCTTCCGGCTGCCTATGAAGATTCATGGGCCGCTCTCAAATGGGTGGAGTCCCATTTAGCCGGAACCGGCCCGGAAGAGTGGCTGAACCGGCATGCAGATTTGAACAGGGTTTTCTTTTCAGGTGATAGCGCTGGGGCTAACATAGCTCACCACATGGGTTTGAGAGTTGGGTCTGAGAAGTTGGTGGGTTTTAAGCTGAATGGGATTGTTCTGGGGCATCCATACTTTTGGGGTGAAGAACCAATAGGCGGAGAGGATCCGAAGTTGGGGGAGTTGGGTTGTGAGAAAGTGTTGGTCTGTGTTGCGGAGAAGGATGCGTTGAAAGATAGGGGGTGGTATTACAGTGAGGTGTTGAAGAAGAGTGGATGGAATGGAGTAGTGGAGGTTTTGGAAACAAAGGAGGAGCAGCATGTGTTCCATTTGATCAATCCTAGTTGTGACAATGCTGTGACCATGCTCAAGAAGATTGTTTCTTTCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

280

Amino Acids

30.96

Weight (kDa)

5.88

Isoelectric Point (pI)

29.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 57 - 178 1.6e-10 Carboxylesterase family
BD-FAE PF20434 66 - 179 4e-13 BD-FAE
Abhydrolase_3 PF07859 74 - 196 3.6e-39 alpha/beta hydrolase fold
Abhydrolase_3 PF07859 212 - 259 1.3e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 343
AccBSI CCGCTC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 45
AciI CCGC 5 cut(s) 147, 237, 387, 606, 662
AclWI GGATC 3 cut(s) 98, 608, 621
AcuI CTGAAG 1 cut(s) 324
AcyI GRCGYC 1 cut(s) 132
AfeI AGCGCT 1 cut(s) 487
AfiI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 605, 623
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 4 cut(s) 122, 460, 679, 712
AjnI CCWGG 1 cut(s) 71
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 4 cut(s) 211, 320, 503, 553
AluI AGCT 4 cut(s) 211, 320, 503, 553
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 2 cut(s) 163, 304
AlwI GGATC 3 cut(s) 98, 608, 621
AlwNI CAGNNNCTG 1 cut(s) 92
Aor51HI AGCGCT 1 cut(s) 487
AoxI GGCC 2 cut(s) 384, 424
ApeKI GCWGC 2 cut(s) 365, 763
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 384, 425
AsuC2I CCSGG 2 cut(s) 205, 428
AsuHPI GGTGA 3 cut(s) 491, 497, 602
BamHI GGATCC 1 cut(s) 613
BanI GGYRCC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 2 cut(s) 352, 775
BccI CCATC 2 cut(s) 107, 717
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 1 cut(s) 73
BclI TGATCA 2 cut(s) 36, 780
BcnI CCSGG 2 cut(s) 205, 428
BcoDI GTCTC 2 cut(s) 163, 304
BfaI CTAG 2 cut(s) 14, 789
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 489
BisI GCNGC 3 cut(s) 366, 387, 764
BlsI GCNGC 3 cut(s) 367, 388, 765
Bme1390I CCNGG 3 cut(s) 73, 205, 428
BmgT120I GGNCC 2 cut(s) 384, 425
BmiI GGNNCC 3 cut(s) 345, 421, 615
BmrFI CCNGG 3 cut(s) 73, 205, 428
BmsI GCATC 3 cut(s) 104, 583, 661
BpuEI CTTGAG 1 cut(s) 800
BpuMI CCSGG 2 cut(s) 205, 428
Bsa29I ATCGAT 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 71, 72
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 605, 623
Bse118I RCCGGY 3 cut(s) 113, 422, 444
BseBI CCWGG 1 cut(s) 73
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 71, 72
BseGI GGATG 4 cut(s) 95, 574, 676, 728
BseLI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 605, 623
BseMII CTCAG 1 cut(s) 522
BseRI GAGGAG 1 cut(s) 773
BseXI GCAGC 2 cut(s) 352, 775
BseYI CCCAGC 1 cut(s) 488
Bsh1285I CGRYCG 2 cut(s) 58, 67
BshFI GGCC 2 cut(s) 386, 426
BshNI GGYRCC 1 cut(s) 343
BshTI ACCGGT 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 102
BsiEI CGRYCG 2 cut(s) 58, 67
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 8 cut(s) 114, 205, 347, 362, 417, 423, 428, 445
BslFI GGGAC 2 cut(s) 74, 391
BslI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 605, 623
BsmAI GTCTC 2 cut(s) 163, 304
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 74, 391
BsnI GGCC 2 cut(s) 386, 426
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 4 cut(s) 36, 103, 613, 780
BspACI CCGC 5 cut(s) 147, 237, 387, 606, 662
BspANI GGCC 2 cut(s) 386, 426
BspCNI CTCAG 1 cut(s) 523
BspDI ATCGAT 1 cut(s) 102
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 3 cut(s) 345, 421, 615
BspPI GGATC 3 cut(s) 98, 608, 621
BspT107I GGYRCC 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 389
BsrFI RCCGGY 3 cut(s) 113, 422, 444
BssAI RCCGGY 3 cut(s) 113, 422, 444
BssECI CCNNGG 2 cut(s) 71, 72
BssMI GATC 4 cut(s) 36, 103, 613, 780
BssNAI GTATAC 1 cut(s) 46
BssNI GRCGYC 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 3 cut(s) 56, 88, 701
Bst6I CTCTTC 4 cut(s) 222, 363, 426, 710
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 1 cut(s) 531
BstF5I GGATG 4 cut(s) 95, 574, 676, 728
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 4 cut(s) 39, 106, 616, 783
BstMAI GTCTC 2 cut(s) 163, 304
BstMBI GATC 4 cut(s) 36, 103, 613, 780
BstMCI CGRYCG 2 cut(s) 58, 67
BstMWI GCNNNNNNNGC 2 cut(s) 445, 500
BstNI CCWGG 1 cut(s) 73
BstNSI RCATGY 2 cut(s) 452, 770
BstSCI CCNGG 3 cut(s) 71, 203, 426
BstSFI CTRYAG 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 352, 775
BstX2I RGATCY 1 cut(s) 613
BstXI CCANNNNNNTGG 1 cut(s) 585
BstYI RGATCY 1 cut(s) 613
BstZ17I GTATAC 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 2 cut(s) 386, 426
BsuTUI ATCGAT 1 cut(s) 102
BtsCI GGATG 4 cut(s) 95, 574, 676, 728
BtsIMutI CAGTG 1 cut(s) 706
Cac8I GCNNGC 1 cut(s) 450
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 834
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 3 cut(s) 113, 422, 444
Cfr13I GGNCC 2 cut(s) 384, 425
ClaI ATCGAT 1 cut(s) 102
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 8 cut(s) 20, 34, 381, 449, 511, 767, 811, 835
DdeI CTNAG 1 cut(s) 531
DpnI GATC 4 cut(s) 38, 105, 615, 782
DpnII GATC 4 cut(s) 36, 103, 613, 780
Eam1104I CTCTTC 4 cut(s) 222, 363, 426, 710
EarI CTCTTC 4 cut(s) 222, 363, 426, 710
EciI GGCGGA 1 cut(s) 621
Eco47III AGCGCT 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 8 cut(s) 23, 37, 384, 452, 514, 770, 814, 838
FaqI GGGAC 2 cut(s) 74, 391
FatI CATG 8 cut(s) 19, 33, 380, 448, 510, 766, 810, 834
FbaI TGATCA 2 cut(s) 36, 780
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 3 cut(s) 366, 387, 764
FokI GGATG 4 cut(s) 82, 561, 683, 735
Fsp4HI GCNGC 3 cut(s) 366, 387, 764
FspBI CTAG 2 cut(s) 14, 789
GlaI GCGC 1 cut(s) 487
GluI GCNGC 3 cut(s) 366, 387, 764
GsaI CCCAGC 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 2 cut(s) 386, 426
HapII CCGG 8 cut(s) 114, 205, 347, 362, 417, 423, 428, 445
HhaI GCGC 1 cut(s) 488
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 8 cut(s) 23, 37, 384, 452, 514, 770, 814, 838
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 2 cut(s) 201, 331
HindII GTYRAC 2 cut(s) 201, 331
HinfI GANTC 4 cut(s) 60, 197, 377, 404
HpaII CCGG 8 cut(s) 114, 205, 347, 362, 417, 423, 428, 445
HphI GGTGA 3 cut(s) 491, 497, 602
Hpy166II GTNNAC 4 cut(s) 46, 201, 226, 331
Hpy188I TCNGA 3 cut(s) 304, 532, 618
Hpy188III TCNNGA 2 cut(s) 817, 835
Hpy8I GTNNAC 4 cut(s) 46, 201, 226, 331
Hpy99I CGWCG 2 cut(s) 11, 137
HpyAV CCTTC 1 cut(s) 661
HpyCH4III ACNGT 3 cut(s) 56, 88, 701
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 4 cut(s) 95, 249, 288, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 500
HpyF3I CTNAG 1 cut(s) 531
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 8 cut(s) 23, 37, 384, 452, 514, 770, 814, 838
HspAI GCGC 1 cut(s) 486
Ksp22I TGATCA 2 cut(s) 36, 780
Kzo9I GATC 4 cut(s) 36, 103, 613, 780
LmnI GCTCC 3 cut(s) 216, 349, 760
Lsp1109I GCAGC 2 cut(s) 352, 775
LweI GCATC 3 cut(s) 104, 583, 661
MaeI CTAG 2 cut(s) 14, 789
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 794, 805
MalI GATC 4 cut(s) 38, 105, 615, 782
MbiI CCGCTC 1 cut(s) 389
MboI GATC 4 cut(s) 36, 103, 613, 780
MboII GAAGA 8 cut(s) 209, 350, 386, 443, 605, 724, 727, 832
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 1 cut(s) 838
MlyI GAGTC 2 cut(s) 206, 413
MnlI CCTC 6 cut(s) 225, 366, 604, 697, 733, 751
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 8 cut(s) 114, 205, 347, 362, 417, 423, 428, 445
MspR9I CCNGG 3 cut(s) 73, 205, 428
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 445, 500
NciI CCSGG 2 cut(s) 205, 428
NdeII GATC 4 cut(s) 36, 103, 613, 780
NlaIII CATG 8 cut(s) 23, 37, 384, 452, 514, 770, 814, 838
NlaIV GGNNCC 3 cut(s) 345, 421, 615
NmuCI GTSAC 2 cut(s) 794, 805
NspI RCATGY 2 cut(s) 452, 770
OliI CACNNNNGTG 1 cut(s) 237
PaeI GCATGC 1 cut(s) 452
PagI TCATGA 1 cut(s) 834
PasI CCCWGGG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 64
PfeI GAWTC 2 cut(s) 60, 377
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 3 cut(s) 367, 388, 765
PleI GAGTC 2 cut(s) 205, 412
PpsI GAGTC 2 cut(s) 205, 412
Psp6I CCWGG 1 cut(s) 71
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 3 cut(s) 345, 421, 615
PspPI GGNCC 2 cut(s) 384, 425
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 1 cut(s) 613
PsyI GACNNNGTC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 3 cut(s) 366, 387, 764
Sau3AI GATC 4 cut(s) 36, 103, 613, 780
Sau96I GGNCC 2 cut(s) 384, 425
SchI GAGTC 2 cut(s) 206, 413
ScrFI CCNGG 3 cut(s) 73, 205, 428
SduI GDGCHC 1 cut(s) 354
SetI ASST 9 cut(s) 135, 213, 322, 341, 481, 505, 555, 708, 744
SfaNI GCATC 3 cut(s) 104, 583, 661
SfcI CTRYAG 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
SphI GCATGC 1 cut(s) 452
Sse9I AATT 1 cut(s) 838
SsiI CCGC 5 cut(s) 147, 237, 387, 606, 662
SspMI CTAG 2 cut(s) 14, 789
StyD4I CCNGG 3 cut(s) 71, 203, 426
TaaI ACNGT 3 cut(s) 56, 88, 701
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 58, 63, 102
TaqII GACCGA 1 cut(s) 81
TasI AATT 1 cut(s) 838
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 2 cut(s) 60, 377
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 1 cut(s) 706
TseFI GTSAC 2 cut(s) 794, 805
TseI GCWGC 2 cut(s) 365, 763
Tsp45I GTSAC 2 cut(s) 794, 805
TspDTI ATGAA 3 cut(s) 369, 387, 823
TspRI CASTG 1 cut(s) 706
Tth111I GACNNNGTC 1 cut(s) 134
XceI RCATGY 2 cut(s) 452, 770
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 2 cut(s) 14, 789
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.