RLG00000014622

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000397
Physical Location & Seq
Reverse (-)
55520214 .. 55521125
912 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014622

Sequence Viewer

Length: 822 bp
ATGAGCGATGAGTTAGCCCATGACTTCTCTCCTCTCTTTAAAATCTACAAAGATGGTCGAATTGAGCGACTCATGGGCACAGATACAGTTCCTCCATCAGTTCATCCCCAAACTGGTGTCGAATCCAAAGACGTCATGATTTCAAACGAAACAGGATTATATGCTAGGCTTCACATCCCCAAATCCACCACAACCTCTTCCGCTAAACTCCCTCTCCTTGTTTACTTCCACGGTGGCGGCTTCTGTGTTGACACTGCCTCTTCTCCTACTTACCACAACTACCTCAACTCCTTAGTAGCGGAAGCTAATGTTGTTGCTGTGTCTGTTGACTTTAGGAGAGTCCCAGAGTACCCTCTGCCTGTTGCCTACAATGATTCCTGGGATGCTCTAAAATGGGTTGCTTCTCTTTCTGATGGAAGTGGCTCTGAGGAGTGGCTAAATAACCATGCAGATTTTCAGAAACTGTTCTTCTCTGGTGATAGTTCTGGTGGTAATATAGCGCATAACATGGCTGTGAAAGTCGGCTCTGAAGGCTTGGTTGGTGTTAAGCTCATAGGGATTGTGCTGGTGCATCCCTTTCTTTGGGGGAAAGAACCAATTGGGGGAGAGTCAACTATGCCTGCAGTTCAAAGAGAGTATCTAGATTCTATGTGGCGTTTTGTGTACCCTTTGACTAGCGGATCTGATGATCCACTTCTCAATCCGGTGAGGTCCTTAGAAAGAGTGGATGGAAAGGGGCTGTGGAGGTCTTGGAAGCAAAGGGGGAGGGCCATGTTTTCCATTTGTATAATCCAACTTGTAACAGTGCTCAGGCCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

274

Amino Acids

30.16

Weight (kDa)

6.06

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 43 - 112 5.3e-08 Carboxylesterase family
BD-FAE PF20434 65 - 175 2.5e-09 BD-FAE
Abhydrolase_3 PF07859 73 - 240 2.3e-40 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AciI CCGC 4 cut(s) 201, 237, 299, 678
AclWI GGATC 2 cut(s) 683, 688
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 2 cut(s) 350, 665
AfiI CCNNNNNNNGG 3 cut(s) 113, 582, 602
AgsI TTSAA 2 cut(s) 144, 629
AjnI CCWGG 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
AluBI AGCT 2 cut(s) 305, 550
AluI AGCT 2 cut(s) 305, 550
Alw21I GWGCWC 1 cut(s) 810
AlwI GGATC 2 cut(s) 683, 688
AlwNI CAGNNNCTG 1 cut(s) 463
AoxI GGCC 2 cut(s) 768, 812
Asp700I GAANNNNTTC 1 cut(s) 464
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 2 cut(s) 711, 768
AsuHPI GGTGA 2 cut(s) 488, 718
AvaII GGWCC 1 cut(s) 711
BaeGI GKGCMC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 810
BccI CCATC 4 cut(s) 47, 103, 407, 722
BciT130I CCWGG 1 cut(s) 379
BfaI CTAG 3 cut(s) 165, 641, 675
BfmI CTRYAG 1 cut(s) 621
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme1390I CCNGG 1 cut(s) 379
Bme18I GGWCC 1 cut(s) 711
BmgT120I GGNCC 2 cut(s) 711, 768
BmrFI CCNGG 1 cut(s) 379
BmsI GCATC 2 cut(s) 373, 580
Bpu10I CCTNAGC 1 cut(s) 809
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 229, 378
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 6 cut(s) 76, 106, 198, 228, 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 582, 602
Bse1I ACTGG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 2 cut(s) 229, 378
BseGI GGATG 5 cut(s) 103, 174, 388, 571, 733
BseLI CCNNNNNNNGG 3 cut(s) 113, 582, 602
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 2 cut(s) 21, 443
BseSI GKGCMC 1 cut(s) 80
BshFI GGCC 2 cut(s) 770, 814
BsiHKAI GWGCWC 1 cut(s) 810
BsiSI CCGG 1 cut(s) 704
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 3 cut(s) 113, 582, 602
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 2 cut(s) 770, 814
Bsp1286I GDGCHC 2 cut(s) 80, 810
Bsp143I GATC 2 cut(s) 680, 688
BspACI CCGC 4 cut(s) 201, 237, 299, 678
BspANI GGCC 2 cut(s) 770, 814
BspCNI CTCAG 2 cut(s) 418, 822
BspHI TCATGA 1 cut(s) 135
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 2 cut(s) 683, 688
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 229, 378
BssMI GATC 2 cut(s) 680, 688
BssNI GRCGYC 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 4 cut(s) 88, 233, 465, 805
Bst6I CTCTTC 2 cut(s) 202, 265
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 4 cut(s) 292, 426, 715, 809
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 5 cut(s) 103, 174, 388, 571, 733
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 2 cut(s) 683, 691
BstMBI GATC 2 cut(s) 680, 688
BstMWI GCNNNNNNNGC 1 cut(s) 531
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 377
BstSFI CTRYAG 1 cut(s) 621
BstSLI GKGCMC 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 680
BstYI RGATCY 1 cut(s) 680
BsuRI GGCC 2 cut(s) 770, 814
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 5 cut(s) 103, 174, 388, 571, 733
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 252, 810
Cac8I GCNNGC 1 cut(s) 621
CaiI CAGNNNCTG 1 cut(s) 463
CciI TCATGA 1 cut(s) 135
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 2 cut(s) 711, 768
Csp6I GTAC 2 cut(s) 349, 664
CviAII CATG 7 cut(s) 20, 73, 136, 446, 508, 772, 816
CviQI GTAC 2 cut(s) 349, 664
DdeI CTNAG 4 cut(s) 292, 426, 715, 809
DpnI GATC 2 cut(s) 682, 690
DpnII GATC 2 cut(s) 680, 688
DraI TTTAAA 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 202, 265
EarI CTCTTC 2 cut(s) 202, 265
Eco47I GGWCC 1 cut(s) 711
Eco57I CTGAAG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 711
EcoRII CCWGG 1 cut(s) 377
FaeI CATG 7 cut(s) 23, 76, 139, 449, 511, 775, 819
FaqI GGGAC 1 cut(s) 326
FatI CATG 7 cut(s) 19, 72, 135, 445, 507, 771, 815
Fnu4HI GCNGC 1 cut(s) 238
FokI GGATG 5 cut(s) 90, 161, 395, 558, 740
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 3 cut(s) 165, 641, 675
GlaI GCGC 1 cut(s) 501
GluI GCNGC 1 cut(s) 238
HaeIII GGCC 2 cut(s) 770, 814
HapII CCGG 1 cut(s) 704
HhaI GCGC 1 cut(s) 502
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 7 cut(s) 23, 76, 139, 449, 511, 775, 819
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 3 cut(s) 250, 328, 612
HindII GTYRAC 3 cut(s) 250, 328, 612
HinfI GANTC 6 cut(s) 69, 122, 339, 374, 608, 644
HpaII CCGG 1 cut(s) 704
HphI GGTGA 2 cut(s) 488, 718
Hpy166II GTNNAC 5 cut(s) 223, 250, 328, 612, 664
Hpy188I TCNGA 5 cut(s) 412, 427, 459, 529, 685
Hpy188III TCNNGA 2 cut(s) 136, 641
Hpy8I GTNNAC 5 cut(s) 223, 250, 328, 612, 664
HpyAV CCTTC 1 cut(s) 524
HpyCH4III ACNGT 4 cut(s) 88, 233, 465, 805
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 3 cut(s) 449, 571, 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 531
HpyF3I CTNAG 4 cut(s) 292, 426, 715, 809
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 7 cut(s) 23, 76, 139, 449, 511, 775, 819
HspAI GCGC 1 cut(s) 500
Kzo9I GATC 2 cut(s) 680, 688
LweI GCATC 2 cut(s) 373, 580
MaeI CTAG 3 cut(s) 165, 641, 675
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 799
MalI GATC 2 cut(s) 682, 690
MboI GATC 2 cut(s) 680, 688
MboII GAAGA 3 cut(s) 189, 252, 460
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 1 cut(s) 680
MhlI GDGCHC 2 cut(s) 80, 810
MluCI AATT 2 cut(s) 60, 597
MlyI GAGTC 3 cut(s) 63, 348, 617
MmeI TCCRAC 1 cut(s) 817
MroXI GAANNNNTTC 1 cut(s) 464
MseI TTAA 2 cut(s) 39, 546
MslI CAYNNNNRTG 1 cut(s) 512
MspI CCGG 1 cut(s) 704
MspR9I CCNGG 1 cut(s) 379
MunI CAATTG 1 cut(s) 597
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 531
NdeII GATC 2 cut(s) 680, 688
NlaIII CATG 7 cut(s) 23, 76, 139, 449, 511, 775, 819
PagI TCATGA 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 3 cut(s) 122, 374, 644
PkrI GCNGC 1 cut(s) 239
PleI GAGTC 3 cut(s) 63, 347, 616
PpsI GAGTC 3 cut(s) 63, 347, 616
PpuMI RGGWCCY 1 cut(s) 711
Psp5II RGGWCCY 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspPI GGNCC 2 cut(s) 711, 768
PspPPI RGGWCCY 1 cut(s) 711
PstI CTGCAG 1 cut(s) 625
PstNI CAGNNNCTG 1 cut(s) 463
PsuI RGATCY 1 cut(s) 680
RsaI GTAC 2 cut(s) 350, 665
RsaNI GTAC 2 cut(s) 349, 664
RseI CAYNNNNRTG 1 cut(s) 512
SaqAI TTAA 2 cut(s) 39, 546
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 2 cut(s) 680, 688
Sau96I GGNCC 2 cut(s) 711, 768
SchI GAGTC 3 cut(s) 63, 348, 617
ScrFI CCNGG 1 cut(s) 379
SduI GDGCHC 2 cut(s) 80, 810
SetI ASST 7 cut(s) 135, 197, 285, 307, 552, 713, 749
SfaNI GCATC 2 cut(s) 373, 580
SfcI CTRYAG 1 cut(s) 621
SinI GGWCC 1 cut(s) 711
SmiMI CAYNNNNRTG 1 cut(s) 512
Sse9I AATT 2 cut(s) 60, 597
SsiI CCGC 4 cut(s) 201, 237, 299, 678
SspMI CTAG 3 cut(s) 165, 641, 675
StyD4I CCNGG 1 cut(s) 377
TaaI ACNGT 4 cut(s) 88, 233, 465, 805
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 58, 120
TasI AATT 2 cut(s) 60, 597
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 3 cut(s) 122, 374, 644
Tru1I TTAA 2 cut(s) 39, 546
Tru9I TTAA 2 cut(s) 39, 546
TscAI CASTG 2 cut(s) 259, 810
TspDTI ATGAA 1 cut(s) 92
TspRI CASTG 2 cut(s) 259, 810
VpaK11BI GGWCC 1 cut(s) 711
XbaI TCTAGA 1 cut(s) 640
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 3 cut(s) 165, 641, 675
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.