FvH4_2g06450

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000397
Physical Location & Seq
Forward (+)
5365320 .. 5369558
4239 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06450.t1

Sequence Viewer

Length: 948 bp
ATGAGCGACGAACTAGCCCAAGATTTTTCTCCCCTCCTCAAAGTATACAAAGACGGTCGAGTCGAGCGGCTCCTAGGCACAGCCAGAGTTCCTGCATCGATCCATCCCCAAACCGGTGTCCAATCCAAAGACGTCGTCATCTCAACCTCACCACCCATCTCTGCAAGGCTTTACATTCCCAAATCATCCGCCTCATCTGAGTCAAGCCAGAAGCTCCCTGTTCTCGTCTACTTTCACGGCGGTGGCTTCTGCATTGAGAGTGCTTTCTCTCCCGCTTACCACAACTACCTCAACTCTTTGGTTTCAGAAGCCAATGTTGTAGCTGTTTCTGTTGACTATAGGTTGGCGCCGGAGCACCCTTTGCCGGCGGCATATGAAGACTCATGGGCGGCTCTAAAATGGGTGGAGTCCCATTTCGCCGGAATGGGCCCGGAAGAGTGGCTGAACCGGCATGCAGATTTGAACCGGGTTTTCTTTTCTGGTGATAGTGCTGGGGCTAACATAGCTCACCACATGGGTTTGAGAGTTGGGTCTGAGAAGCCGGTGGGTTTTAAGCTGAATGGAATTGTTCTGGTGCATCCCTACTTTTGGGGTGAAGAACCAATAGGGGGAGAGCTAGCTTTGGGTGAGAGTCATAGAAATTTGTTGGCTACTTTGTGGAGGTTTTGTTACCCTTTGACTAGTGGATCCGATGATCCGTTGTACAATCCGGGTAAGGATCCGAAGTTGGGGGAGATGGGTTGTGAGAAAGTATTGGTCTGTGTTGCGGAGAAGGATTCGTTGAAAGATAGGGGGTGGTATTACAGTGAGTTGTTGAAGAAGAGTGGATGGAATGGAGTTGTGGAGGTTATGGAAGCAAAGGAGGAGCAGCATGTGTTCCATTTGTTCAATCCTACTTGTGACAATGCTGTGGCCATGCTCAAGAAGATCGTATCTTTCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

316

Amino Acids

34.78

Weight (kDa)

5.84

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 58 - 172 4.7e-11 Carboxylesterase family
BD-FAE PF20434 66 - 177 8.1e-13 BD-FAE
Abhydrolase_3 PF07859 75 - 294 6.7e-55 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 346
AccBSI CCGCTC 1 cut(s) 67
AccI GTMKAC 2 cut(s) 45, 228
AciI CCGC 7 cut(s) 67, 189, 240, 273, 368, 389, 767
AclWI GGATC 6 cut(s) 94, 681, 689, 694, 713, 726
AcoI YGGCCR 1 cut(s) 912
AcsI RAATTY 1 cut(s) 640
AcyI GRCGYC 2 cut(s) 132, 347
AfaI GTAC 1 cut(s) 704
AfiI CCNNNNNNNGG 5 cut(s) 113, 364, 588, 608, 728
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 4 cut(s) 463, 784, 817, 889
AhlI ACTAGT 1 cut(s) 680
AleI CACNNNNGTG 1 cut(s) 240
AluBI AGCT 6 cut(s) 214, 323, 506, 556, 616, 620
AluI AGCT 6 cut(s) 214, 323, 506, 556, 616, 620
Alw21I GWGCWC 1 cut(s) 357
AlwI GGATC 6 cut(s) 94, 681, 689, 694, 713, 726
AoxI GGCC 2 cut(s) 427, 912
ApaI GGGCCC 1 cut(s) 431
ApeKI GCWGC 1 cut(s) 868
ApoI RAATTY 1 cut(s) 640
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 113
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 2 cut(s) 427, 428
AsuC2I CCSGG 3 cut(s) 431, 467, 711
AsuHPI GGTGA 5 cut(s) 141, 494, 500, 605, 638
AsuNHI GCTAGC 1 cut(s) 616
AvrII CCTAGG 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 431
BalI TGGCCA 1 cut(s) 914
BamHI GGATCC 2 cut(s) 686, 718
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 880
BccI CCATC 4 cut(s) 111, 164, 730, 822
BceAI ACGGC 1 cut(s) 253
BcnI CCSGG 3 cut(s) 431, 467, 711
BcuI ACTAGT 1 cut(s) 680
BfaI CTAG 4 cut(s) 14, 74, 617, 681
BfmI CTRYAG 1 cut(s) 337
BfoI RGCGCY 1 cut(s) 350
BisI GCNGC 4 cut(s) 68, 369, 390, 869
BlnI CCTAGG 1 cut(s) 73
BlsI GCNGC 4 cut(s) 69, 370, 391, 870
Bme1390I CCNGG 3 cut(s) 431, 467, 711
BmgT120I GGNCC 2 cut(s) 427, 428
BmiI GGNNCC 5 cut(s) 71, 348, 429, 688, 720
BmrFI CCNGG 3 cut(s) 431, 467, 711
BmsI GCATC 2 cut(s) 104, 586
BmtI GCTAGC 1 cut(s) 620
BpiI GAAGAC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 905
BpuMI CCSGG 3 cut(s) 431, 467, 711
Bsa29I ATCGAT 1 cut(s) 98
BsaHI GRCGYC 2 cut(s) 132, 347
BsaJI CCNNGG 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 2 cut(s) 652, 682
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 364, 588, 608, 728
Bse118I RCCGGY 4 cut(s) 113, 364, 447, 541
BseCI ATCGAT 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 4 cut(s) 103, 185, 577, 833
BseLI CCNNNNNNNGG 5 cut(s) 113, 364, 588, 608, 728
BseMII CTCAG 2 cut(s) 189, 525
BseRI GAGGAG 2 cut(s) 26, 878
BseSI GKGCMC 1 cut(s) 431
BseXI GCAGC 1 cut(s) 880
BseYI CCCAGC 1 cut(s) 491
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 429, 914
BshNI GGYRCC 1 cut(s) 346
BshTI ACCGGT 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 9 cut(s) 114, 350, 365, 420, 431, 448, 466, 542, 710
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 5 cut(s) 113, 364, 588, 608, 728
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 2 cut(s) 429, 914
Bsp120I GGGCCC 1 cut(s) 427
Bsp1286I GDGCHC 2 cut(s) 357, 431
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 5 cut(s) 99, 686, 694, 718, 927
BspACI CCGC 7 cut(s) 67, 189, 240, 273, 368, 389, 767
BspANI GGCC 2 cut(s) 429, 914
BspCNI CTCAG 2 cut(s) 190, 526
BspDI ATCGAT 1 cut(s) 98
BspHI TCATGA 1 cut(s) 939
BspLI GGNNCC 5 cut(s) 71, 348, 429, 688, 720
BspOI GCTAGC 1 cut(s) 620
BspPI GGATC 6 cut(s) 94, 681, 689, 694, 713, 726
BspT107I GGYRCC 1 cut(s) 346
BsrBI CCGCTC 1 cut(s) 67
BsrFI RCCGGY 4 cut(s) 113, 364, 447, 541
BsrGI TGTACA 1 cut(s) 702
BssAI RCCGGY 4 cut(s) 113, 364, 447, 541
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 5 cut(s) 99, 686, 694, 718, 927
BssNAI GTATAC 1 cut(s) 46
BssNI GRCGYC 2 cut(s) 132, 347
BssT1I CCWWGG 1 cut(s) 73
Bst1107I GTATAC 1 cut(s) 46
Bst4CI ACNGT 2 cut(s) 56, 806
Bst6I CTCTTC 2 cut(s) 429, 815
BstACI GRCGYC 2 cut(s) 132, 347
BstAPI GCANNNNNTGC 1 cut(s) 361
BstAUI TGTACA 1 cut(s) 702
BstC8I GCNNGC 3 cut(s) 366, 453, 618
BstDEI CTNAG 2 cut(s) 198, 534
BstF5I GGATG 4 cut(s) 103, 185, 577, 833
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 5 cut(s) 102, 689, 697, 721, 930
BstMBI GATC 5 cut(s) 99, 686, 694, 718, 927
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 3 cut(s) 361, 448, 503
BstNSI RCATGY 2 cut(s) 455, 875
BstSCI CCNGG 3 cut(s) 429, 465, 709
BstSFI CTRYAG 1 cut(s) 337
BstSLI GKGCMC 1 cut(s) 431
BstV1I GCAGC 1 cut(s) 880
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 2 cut(s) 686, 718
BstYI RGATCY 2 cut(s) 686, 718
BstZ17I GTATAC 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 98
BsuRI GGCC 2 cut(s) 429, 914
BsuTUI ATCGAT 1 cut(s) 98
BtsCI GGATG 4 cut(s) 103, 185, 577, 833
BtsIMutI CAGTG 1 cut(s) 811
Cac8I GCNNGC 3 cut(s) 366, 453, 618
CciI TCATGA 1 cut(s) 939
CfoI GCGC 1 cut(s) 349
Cfr10I RCCGGY 4 cut(s) 113, 364, 447, 541
Cfr13I GGNCC 2 cut(s) 427, 428
ClaI ATCGAT 1 cut(s) 98
Csp6I GTAC 1 cut(s) 703
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 6 cut(s) 384, 452, 514, 872, 916, 940
CviQI GTAC 1 cut(s) 703
DdeI CTNAG 2 cut(s) 198, 534
DinI GGCGCC 1 cut(s) 348
DpnI GATC 5 cut(s) 101, 688, 696, 720, 929
DpnII GATC 5 cut(s) 99, 686, 694, 718, 927
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 1 cut(s) 912
Eam1104I CTCTTC 2 cut(s) 429, 815
EarI CTCTTC 2 cut(s) 429, 815
EciI GGCGGA 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 431
EcoT14I CCWWGG 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 431
EgeI GGCGCC 1 cut(s) 348
EheI GGCGCC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 6 cut(s) 387, 455, 517, 875, 919, 943
FaqI GGGAC 1 cut(s) 394
FatI CATG 6 cut(s) 383, 451, 513, 871, 915, 939
FauI CCCGC 1 cut(s) 280
FauNDI CATATG 1 cut(s) 373
FblI GTMKAC 2 cut(s) 45, 228
Fnu4HI GCNGC 4 cut(s) 68, 369, 390, 869
FokI GGATG 4 cut(s) 90, 172, 564, 840
FriOI GRGCYC 1 cut(s) 431
Fsp4HI GCNGC 4 cut(s) 68, 369, 390, 869
FspBI CTAG 4 cut(s) 14, 74, 617, 681
GlaI GCGC 1 cut(s) 348
GluI GCNGC 4 cut(s) 68, 369, 390, 869
GsaI CCCAGC 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 2 cut(s) 429, 914
HapII CCGG 9 cut(s) 114, 350, 365, 420, 431, 448, 466, 542, 710
HhaI GCGC 1 cut(s) 349
Hin1I GRCGYC 2 cut(s) 132, 347
Hin1II CATG 6 cut(s) 387, 455, 517, 875, 919, 943
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 6 cut(s) 60, 200, 380, 407, 631, 776
HpaII CCGG 9 cut(s) 114, 350, 365, 420, 431, 448, 466, 542, 710
HphI GGTGA 5 cut(s) 141, 494, 500, 605, 638
Hpy166II GTNNAC 3 cut(s) 46, 229, 334
Hpy188I TCNGA 5 cut(s) 199, 307, 535, 691, 723
Hpy188III TCNNGA 2 cut(s) 922, 940
Hpy8I GTNNAC 3 cut(s) 46, 229, 334
Hpy99I CGWCG 2 cut(s) 11, 137
HpyAV CCTTC 1 cut(s) 766
HpyCH4III ACNGT 2 cut(s) 56, 806
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 5 cut(s) 95, 164, 252, 455, 577
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 448, 503
HpyF3I CTNAG 2 cut(s) 198, 534
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 2 cut(s) 132, 347
Hsp92II CATG 6 cut(s) 387, 455, 517, 875, 919, 943
HspAI GCGC 1 cut(s) 347
KasI GGCGCC 1 cut(s) 346
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
Kzo9I GATC 5 cut(s) 99, 686, 694, 718, 927
LmnI GCTCC 4 cut(s) 75, 219, 352, 865
Lsp1109I GCAGC 1 cut(s) 880
LweI GCATC 2 cut(s) 104, 586
MaeI CTAG 4 cut(s) 14, 74, 617, 681
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 668, 899
MalI GATC 5 cut(s) 101, 688, 696, 720, 929
MbiI CCGCTC 1 cut(s) 67
MboI GATC 5 cut(s) 99, 686, 694, 718, 927
MboII GAAGA 6 cut(s) 389, 446, 608, 829, 832, 937
MflI RGATCY 2 cut(s) 686, 718
MhlI GDGCHC 2 cut(s) 357, 431
MlsI TGGCCA 1 cut(s) 914
MluCI AATT 3 cut(s) 564, 640, 943
MluNI TGGCCA 1 cut(s) 914
Mly113I GGCGCC 1 cut(s) 347
MlyI GAGTC 5 cut(s) 69, 209, 374, 416, 640
MnlI CCTC 8 cut(s) 44, 47, 157, 202, 299, 654, 838, 856
Mox20I TGGCCA 1 cut(s) 914
MroNI GCCGGC 1 cut(s) 364
MscI TGGCCA 1 cut(s) 914
MseI TTAA 1 cut(s) 552
MslI CAYNNNNRTG 1 cut(s) 240
Msp20I TGGCCA 1 cut(s) 914
MspI CCGG 9 cut(s) 114, 350, 365, 420, 431, 448, 466, 542, 710
MspR9I CCNGG 3 cut(s) 431, 467, 711
MwoI GCNNNNNNNGC 3 cut(s) 361, 448, 503
NaeI GCCGGC 1 cut(s) 366
NarI GGCGCC 1 cut(s) 347
NciI CCSGG 3 cut(s) 431, 467, 711
NdeI CATATG 1 cut(s) 373
NdeII GATC 5 cut(s) 99, 686, 694, 718, 927
NgoMIV GCCGGC 1 cut(s) 364
NheI GCTAGC 1 cut(s) 616
NlaIII CATG 6 cut(s) 387, 455, 517, 875, 919, 943
NlaIV GGNNCC 5 cut(s) 71, 348, 429, 688, 720
NmuCI GTSAC 1 cut(s) 899
NspI RCATGY 2 cut(s) 455, 875
OliI CACNNNNGTG 1 cut(s) 240
PaeI GCATGC 1 cut(s) 455
PagI TCATGA 1 cut(s) 939
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PdiI GCCGGC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 776
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 4 cut(s) 69, 370, 391, 870
PleI GAGTC 5 cut(s) 68, 208, 374, 415, 639
PluTI GGCGCC 1 cut(s) 350
PpsI GAGTC 5 cut(s) 68, 208, 374, 415, 639
PspFI CCCAGC 1 cut(s) 491
PspN4I GGNNCC 5 cut(s) 71, 348, 429, 688, 720
PspOMI GGGCCC 1 cut(s) 427
PspPI GGNCC 2 cut(s) 427, 428
PsuI RGATCY 2 cut(s) 686, 718
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 1 cut(s) 704
RsaNI GTAC 1 cut(s) 703
RseI CAYNNNNRTG 1 cut(s) 240
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 4 cut(s) 68, 369, 390, 869
Sau3AI GATC 5 cut(s) 99, 686, 694, 718, 927
Sau96I GGNCC 2 cut(s) 427, 428
SchI GAGTC 5 cut(s) 69, 209, 374, 416, 640
ScrFI CCNGG 3 cut(s) 431, 467, 711
SduI GDGCHC 2 cut(s) 357, 431
SfaNI GCATC 2 cut(s) 104, 586
SfcI CTRYAG 1 cut(s) 337
SfoI GGCGCC 1 cut(s) 348
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SpeI ACTAGT 1 cut(s) 680
SphI GCATGC 1 cut(s) 455
Sse9I AATT 3 cut(s) 564, 640, 943
SsiI CCGC 7 cut(s) 67, 189, 240, 273, 368, 389, 767
SspDI GGCGCC 1 cut(s) 346
SspMI CTAG 4 cut(s) 14, 74, 617, 681
StyD4I CCNGG 3 cut(s) 429, 465, 709
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 2 cut(s) 56, 806
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 58, 63, 98
TasI AATT 3 cut(s) 564, 640, 943
TatI WGTACW 1 cut(s) 702
TauI GCSGC 3 cut(s) 70, 371, 392
TfiI GAWTC 1 cut(s) 776
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 1 cut(s) 811
TseFI GTSAC 1 cut(s) 899
TseI GCWGC 1 cut(s) 868
Tsp45I GTSAC 1 cut(s) 899
TspDTI ATGAA 2 cut(s) 390, 928
TspGWI ACGGA 1 cut(s) 687
TspRI CASTG 1 cut(s) 811
Tth111I GACNNNGTC 1 cut(s) 134
XapI RAATTY 1 cut(s) 640
XceI RCATGY 2 cut(s) 455, 875
XmaJI CCTAGG 1 cut(s) 73
XmiI GTMKAC 2 cut(s) 45, 228
XspI CTAG 4 cut(s) 14, 74, 617, 681
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.