MD05G1191100.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000397
Physical Location & Seq
Reverse (-)
31979032 .. 31979991
960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1191100.v1.1.491

Sequence Viewer

Length: 960 bp
ATGAGCAACGAAGAACTATGTTATGACTTTTCTCCAATGATCAAGGCATACAAAGACGGTCGAATCGAGAGACTCCTAGGCACAGCCACAGTTCCTCCATCAACACAACCCGAAACCGGAGTCCAATCCAAAGACGTTGTCATCTCCCAACAACCAGCAATATCTGTAAGGCTCTACATCCCCAAATCCGCTGCCACCAAACTCCCTCTTCTCGTTTACTTTCACGGCGGTGGCTTCTGCATCGAAAGCGCTTCCTCCCCCACGTATCACGACTACCTCAACTCCTTAGTCTCTGAAGCCAATGTTGTTGCCGTCTCTGTTGAGTACAGGCTTGCCCCGGAGCACCCTGTCCCGGCTGCCTACGACGATTCATGGGCTGCTCTCAAATGGGTGGCTTCCCATTTTGATGGAACGCGAAAAGGCGGTGAAAAAGAAGACGAAGATTGGATAACTAGCTATGCGGATTCGCAGCGCGTGTTCTTCGCTGGGGACAGCGCCGGAGCTAATATAGCGCACCGTATGGGATTGAAAGTAGGATCGGACGGGCTGGTTGGTGTTAAGCTGATTGGGGTTGTGTTGGTCCATCCATATTTCTGGGGGTCAGAGTCAATTGGGGTAGAGCTCAATGCCCCTGCAGCGATGAGAGAGTTTATGGCAGCCATGTGGCGTTTTGTCAACCCGTTGAGTAGTGGATCCGATGACCCGCTCATGAACCCCGAAAAGGATCCAAAACTGGGGAAATTGGGGTGTGGGAAAGTGGTAGTTTTCGTGGCGGAAAAAGATGTGTTGAAAGATAGGGGATGGTATTACGGGGAGGTGCTGAGAAAGAGTGGATGGAATGGGGTTGTGGAGGTGATGGAAGCAAAAGGGGAGGAGCATTGTTTCCATTTAGACGATCTAACTTGTGAGAATGCTGTGGCCATGCAGAAGAAGATAGTCTCTTTCTTGAACCAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

320

Amino Acids

34.99

Weight (kDa)

5.33

Isoelectric Point (pI)

32.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 56 - 113 1.2e-08 Carboxylesterase family
BD-FAE PF20434 58 - 178 3.5e-12 BD-FAE
Abhydrolase_3 PF07859 71 - 296 7.3e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 706
AccII CGCG 2 cut(s) 415, 474
AciI CCGC 6 cut(s) 189, 228, 423, 461, 704, 773
AclWI GGATC 5 cut(s) 544, 687, 700, 719, 732
AcoI YGGCCR 1 cut(s) 918
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 1 cut(s) 326
AfeI AGCGCT 1 cut(s) 250
AfiI CCNNNNNNNGG 4 cut(s) 116, 352, 721, 734
AgsI TTSAA 3 cut(s) 529, 790, 949
AjnI CCWGG 1 cut(s) 951
AleI CACNNNNGTG 1 cut(s) 228
AluBI AGCT 4 cut(s) 456, 503, 562, 622
AluI AGCT 4 cut(s) 456, 503, 562, 622
Alw21I GWGCWC 2 cut(s) 345, 624
Alw26I GTCTC 4 cut(s) 64, 295, 319, 943
AlwI GGATC 5 cut(s) 544, 687, 700, 719, 732
Aor51HI AGCGCT 1 cut(s) 250
AoxI GGCC 1 cut(s) 918
ApeKI GCWGC 6 cut(s) 191, 356, 377, 469, 635, 656
ArsI GACNNNNNNTTYG 4 cut(s) 105, 123, 137, 155
AspA2I CCTAGG 1 cut(s) 76
AspLEI GCGC 4 cut(s) 251, 474, 497, 514
AspS9I GGNCC 1 cut(s) 580
AsuC2I CCSGG 2 cut(s) 338, 353
AsuHPI GGTGA 2 cut(s) 437, 865
AvaII GGWCC 1 cut(s) 580
AvrII CCTAGG 1 cut(s) 76
BalI TGGCCA 1 cut(s) 920
BamHI GGATCC 2 cut(s) 692, 724
BanII GRGCYC 1 cut(s) 624
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 345, 624
BbvI GCAGC 6 cut(s) 178, 343, 364, 481, 647, 668
BccI CCATC 6 cut(s) 106, 401, 591, 795, 828, 850
BceAI ACGGC 2 cut(s) 241, 296
BciT130I CCWGG 1 cut(s) 953
BclI TGATCA 1 cut(s) 39
BcnI CCSGG 2 cut(s) 338, 353
BcoDI GTCTC 4 cut(s) 64, 295, 319, 943
BfaI CTAG 2 cut(s) 77, 453
BfmI CTRYAG 1 cut(s) 633
BfoI RGCGCY 2 cut(s) 252, 498
BisI GCNGC 6 cut(s) 192, 357, 378, 470, 636, 657
BlnI CCTAGG 1 cut(s) 76
BlsI GCNGC 6 cut(s) 193, 358, 379, 471, 637, 658
Bme1390I CCNGG 3 cut(s) 338, 353, 953
Bme18I GGWCC 1 cut(s) 580
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 2 cut(s) 694, 726
BmrFI CCNGG 3 cut(s) 338, 353, 953
BmrI ACTGGG 1 cut(s) 743
BmsI GCATC 1 cut(s) 249
BmuI ACTGGG 1 cut(s) 743
BpiI GAAGAC 1 cut(s) 441
BpuMI CCSGG 2 cut(s) 338, 353
BsaAI YACGTR 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 76, 336
BsaWI WCCGGW 1 cut(s) 116
BsaXI ACNNNNNCTCC 4 cut(s) 79, 109, 266, 296
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 352, 721, 734
Bse1I ACTGG 1 cut(s) 738
BseBI CCWGG 1 cut(s) 953
BseDI CCNNGG 2 cut(s) 76, 336
BseGI GGATG 4 cut(s) 177, 583, 806, 839
BseLI CCNNNNNNNGG 4 cut(s) 116, 352, 721, 734
BseMII CTCAG 1 cut(s) 812
BseNI ACTGG 1 cut(s) 738
BseRI GAGGAG 1 cut(s) 887
BseXI GCAGC 6 cut(s) 178, 343, 364, 481, 647, 668
BseYI CCCAGC 1 cut(s) 485
Bsh1236I CGCG 2 cut(s) 415, 474
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 1 cut(s) 920
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 2 cut(s) 345, 624
BsiSI CCGG 4 cut(s) 117, 338, 353, 498
BslFI GGGAC 2 cut(s) 335, 503
BslI CCNNNNNNNGG 4 cut(s) 116, 352, 721, 734
BsmAI GTCTC 4 cut(s) 64, 295, 319, 943
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 2 cut(s) 335, 503
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 1 cut(s) 920
Bsp1286I GDGCHC 2 cut(s) 345, 624
Bsp143I GATC 5 cut(s) 39, 536, 692, 724, 895
BspACI CCGC 6 cut(s) 189, 228, 423, 461, 704, 773
BspANI GGCC 1 cut(s) 920
BspCNI CTCAG 1 cut(s) 813
BspFNI CGCG 2 cut(s) 415, 474
BspHI TCATGA 1 cut(s) 708
BspLI GGNNCC 2 cut(s) 694, 726
BspMAI CTGCAG 1 cut(s) 637
BspPI GGATC 5 cut(s) 544, 687, 700, 719, 732
BsrBI CCGCTC 1 cut(s) 706
BsrI ACTGG 1 cut(s) 738
BssECI CCNNGG 2 cut(s) 76, 336
BssMI GATC 5 cut(s) 39, 536, 692, 724, 895
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 1 cut(s) 953
Bst4CI ACNGT 3 cut(s) 59, 91, 518
Bst6I CTCTTC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 264
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 2 cut(s) 286, 821
BstF5I GGATG 4 cut(s) 177, 583, 806, 839
BstFNI CGCG 2 cut(s) 415, 474
BstH2I RGCGCY 2 cut(s) 252, 498
BstHHI GCGC 4 cut(s) 251, 474, 497, 514
BstKTI GATC 5 cut(s) 42, 539, 695, 727, 898
BstMAI GTCTC 4 cut(s) 64, 295, 319, 943
BstMBI GATC 5 cut(s) 39, 536, 692, 724, 895
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 246, 509, 635
BstNI CCWGG 1 cut(s) 953
BstSCI CCNGG 3 cut(s) 336, 351, 951
BstSFI CTRYAG 1 cut(s) 633
BstUI CGCG 2 cut(s) 415, 474
BstV1I GCAGC 6 cut(s) 178, 343, 364, 481, 647, 668
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 2 cut(s) 692, 724
BstXI CCANNNNNNTGG 2 cut(s) 407, 594
BstYI RGATCY 2 cut(s) 692, 724
BsuRI GGCC 1 cut(s) 920
BtgZI GCGATG 1 cut(s) 653
BtsCI GGATG 4 cut(s) 177, 583, 806, 839
Cac8I GCNNGC 1 cut(s) 333
CciI TCATGA 1 cut(s) 708
CfoI GCGC 4 cut(s) 251, 474, 497, 514
Cfr13I GGNCC 1 cut(s) 580
CsiI ACCWGGT 1 cut(s) 951
Csp6I GTAC 1 cut(s) 325
CviAII CATG 4 cut(s) 372, 661, 709, 922
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 2 cut(s) 286, 821
DpnI GATC 5 cut(s) 41, 538, 694, 726, 897
DpnII GATC 5 cut(s) 39, 536, 692, 724, 895
EaeI YGGCCR 1 cut(s) 918
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
EciI GGCGGA 1 cut(s) 788
Ecl136II GAGCTC 1 cut(s) 622
Eco130I CCWWGG 1 cut(s) 76
Eco24I GRGCYC 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 580
Eco47III AGCGCT 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 951
EcoT14I CCWWGG 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 4 cut(s) 375, 664, 712, 925
FaqI GGGAC 2 cut(s) 335, 503
FatI CATG 4 cut(s) 371, 660, 708, 921
FauI CCCGC 1 cut(s) 711
FbaI TGATCA 1 cut(s) 39
Fnu4HI GCNGC 6 cut(s) 192, 357, 378, 470, 636, 657
FokI GGATG 4 cut(s) 164, 570, 813, 846
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 6 cut(s) 192, 357, 378, 470, 636, 657
FspBI CTAG 2 cut(s) 77, 453
GlaI GCGC 4 cut(s) 250, 473, 496, 513
GluI GCNGC 6 cut(s) 192, 357, 378, 470, 636, 657
GsaI CCCAGC 1 cut(s) 489
HaeII RGCGCY 2 cut(s) 252, 498
HaeIII GGCC 1 cut(s) 920
HapII CCGG 4 cut(s) 117, 338, 353, 498
HhaI GCGC 4 cut(s) 251, 474, 497, 514
Hin1II CATG 4 cut(s) 375, 664, 712, 925
Hin6I GCGC 4 cut(s) 249, 472, 495, 512
HinP1I GCGC 4 cut(s) 249, 472, 495, 512
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HinfI GANTC 6 cut(s) 63, 72, 120, 368, 464, 605
HpaII CCGG 4 cut(s) 117, 338, 353, 498
HphI GGTGA 2 cut(s) 437, 865
Hpy166II GTNNAC 2 cut(s) 217, 676
Hpy188I TCNGA 4 cut(s) 295, 541, 604, 697
Hpy188III TCNNGA 4 cut(s) 67, 269, 709, 946
Hpy8I GTNNAC 2 cut(s) 217, 676
Hpy99I CGWCG 1 cut(s) 368
HpyCH4III ACNGT 3 cut(s) 59, 91, 518
HpyCH4IV ACGT 2 cut(s) 135, 263
HpyCH4V TGCA 3 cut(s) 240, 635, 925
HpyF10VI GCNNNNNNNGC 3 cut(s) 246, 509, 635
HpyF3I CTNAG 2 cut(s) 286, 821
HpySE526I ACGT 2 cut(s) 135, 263
Hsp92II CATG 4 cut(s) 375, 664, 712, 925
HspAI GCGC 4 cut(s) 249, 472, 495, 512
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 5 cut(s) 39, 536, 692, 724, 895
LmnI GCTCC 3 cut(s) 340, 500, 874
Lsp1109I GCAGC 6 cut(s) 178, 343, 364, 481, 647, 668
LweI GCATC 1 cut(s) 249
MabI ACCWGGT 1 cut(s) 951
MaeI CTAG 2 cut(s) 77, 453
MaeII ACGT 2 cut(s) 135, 263
MalI GATC 5 cut(s) 41, 538, 694, 726, 897
MbiI CCGCTC 1 cut(s) 706
MboI GATC 5 cut(s) 39, 536, 692, 724, 895
MboII GAAGA 7 cut(s) 23, 200, 446, 452, 472, 940, 943
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 2 cut(s) 692, 724
MhlI GDGCHC 2 cut(s) 345, 624
MlsI TGGCCA 1 cut(s) 920
MluCI AATT 2 cut(s) 609, 740
MluNI TGGCCA 1 cut(s) 920
MlyI GAGTC 3 cut(s) 66, 129, 614
MnlI CCTC 7 cut(s) 105, 216, 265, 287, 808, 844, 865
Mox20I TGGCCA 1 cut(s) 920
MscI TGGCCA 1 cut(s) 920
MseI TTAA 1 cut(s) 558
MslI CAYNNNNRTG 2 cut(s) 228, 405
Msp20I TGGCCA 1 cut(s) 920
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 4 cut(s) 117, 338, 353, 498
MspR9I CCNGG 3 cut(s) 338, 353, 953
MunI CAATTG 1 cut(s) 609
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 1 cut(s) 953
MvnI CGCG 2 cut(s) 415, 474
MwoI GCNNNNNNNGC 3 cut(s) 246, 509, 635
NciI CCSGG 2 cut(s) 338, 353
NdeII GATC 5 cut(s) 39, 536, 692, 724, 895
NlaIII CATG 4 cut(s) 375, 664, 712, 925
NlaIV GGNNCC 2 cut(s) 694, 726
OliI CACNNNNGTG 1 cut(s) 228
PagI TCATGA 1 cut(s) 708
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 916
PfeI GAWTC 3 cut(s) 63, 368, 464
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 6 cut(s) 193, 358, 379, 471, 637, 658
PleI GAGTC 3 cut(s) 66, 128, 613
PpsI GAGTC 3 cut(s) 66, 128, 613
Ppu21I YACGTR 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 624
Psp6I CCWGG 1 cut(s) 951
PspFI CCCAGC 1 cut(s) 485
PspGI CCWGG 1 cut(s) 951
PspN4I GGNNCC 2 cut(s) 694, 726
PspPI GGNCC 1 cut(s) 580
PstI CTGCAG 1 cut(s) 637
PsuI RGATCY 2 cut(s) 692, 724
PsyI GACNNNGTC 1 cut(s) 137
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 2 cut(s) 228, 405
SacI GAGCTC 1 cut(s) 624
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 6 cut(s) 192, 357, 378, 470, 636, 657
Sau3AI GATC 5 cut(s) 39, 536, 692, 724, 895
Sau96I GGNCC 1 cut(s) 580
SchI GAGTC 3 cut(s) 66, 129, 614
ScrFI CCNGG 3 cut(s) 338, 353, 953
SduI GDGCHC 2 cut(s) 345, 624
SexAI ACCWGGT 1 cut(s) 951
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 1 cut(s) 580
SmiMI CAYNNNNRTG 2 cut(s) 228, 405
Sse9I AATT 2 cut(s) 609, 740
SsiI CCGC 6 cut(s) 189, 228, 423, 461, 704, 773
SspMI CTAG 2 cut(s) 77, 453
SstI GAGCTC 1 cut(s) 624
StyD4I CCNGG 3 cut(s) 336, 351, 951
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 3 cut(s) 59, 91, 518
TaiI ACGT 2 cut(s) 138, 266
TaqI TCGA 3 cut(s) 61, 66, 243
TasI AATT 2 cut(s) 609, 740
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 3 cut(s) 63, 368, 464
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TseI GCWGC 6 cut(s) 191, 356, 377, 469, 635, 656
TspDTI ATGAA 2 cut(s) 360, 725
Tth111I GACNNNGTC 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 580
XmaJI CCTAGG 1 cut(s) 76
XspI CTAG 2 cut(s) 77, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.