RchiOBHm_Chr6g0258291

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000397
Physical Location & Seq
Forward (+)
13672504 .. 13673699
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23161

Sequence Viewer

Length: 945 bp
ATGAGCGACGAACTAGCCCATGATTTCTCTCCCATCATCAAAGTATACAAAGACGGTCGAATCGACCGCCTCCTAGGCACATCCACAGTCCCTGCATGCATTGATCCCCAAACCGGTGTTCAATCCAAAGACGTCGTCATCTCAACCGCACCACCCATCTCTGCAAGGCTTTATATTCCCAAATCCACCGCAACCGAGTCAGCCCAGAAGCTCCCTCTTCTGGTTTACTTTCACGGCGGTGGCTTTTGCGTTGAAAGCGCTTTCTCTCCCACTTATCACAACTACCTCAACTCTTTAGTCTCCGAGGCCAAAGTTGCAGCTGTTTCTGTTGAATATAGGCTGGCGCCGGAGGATCCACTCCCGGCTGCCTATGAAGATTCGTGGGCCGCACTCAAATGGGTGGAGTCCCATCTCGCAGGAAACGGCCCGGAAGACTGGCTGAACCGGTATGCAGATTTGAACCGGGTTTTCTTTTCAGGTGATAGTGCTGGGGCTAACATAGCTCACCACATGGGTTTGAGAGTTGGATCTGAGAAGTTGGTGGGTTTTAAGCTGAATGGGATTGTTCTGGTGCATCCCTACTTTTGGGGTGAAGAACCAATAGGGGGAGAAGTAGCTTTGGCCGAGAGTCTTAGAAAGATGGTGCCTGCTTTGTGGCGGTTTGCTTACCCTTTGACTAGTGGATCCGATGACCCGTTTTTTAATCCGGGTAAGGATCCGAAGTTGGGGGAGTTGGGTTGTGAGAAAGTTTTGGTCTGTGTTGCGGAGAAGGATGCGTTGAAAGATAGGGGGTGGTATTACAGTGAGGTGTTGAAGAAGAGTGGATGGAATGGAGTTGTGGAGGTATCAGAAGCGAAGGAGGAGCAGCATGTGTTCCATTTGTCCAATGCTACTTGTGACAATGCTGTGACCATGCTCAAGAAGATCGTTTCTTTCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

314

Amino Acids

34.47

Weight (kDa)

5.51

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 58 - 178 2.3e-08 Carboxylesterase family
BD-FAE PF20434 66 - 179 2e-12 BD-FAE
Abhydrolase_3 PF07859 74 - 293 9.7e-53 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 2 cut(s) 343, 643
AccI GTMKAC 1 cut(s) 45
AciI CCGC 7 cut(s) 67, 147, 189, 237, 387, 658, 764
AclWI GGATC 8 cut(s) 98, 347, 360, 535, 678, 691, 710, 723
AcoI YGGCCR 1 cut(s) 621
AcyI GRCGYC 2 cut(s) 132, 344
AfeI AGCGCT 1 cut(s) 259
AfiI CCNNNNNNNGG 5 cut(s) 113, 220, 585, 605, 725
AgeI ACCGGT 2 cut(s) 113, 444
AgsI TTSAA 6 cut(s) 122, 254, 332, 460, 781, 814
AhlI ACTAGT 1 cut(s) 677
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 5 cut(s) 211, 320, 503, 553, 617
AluI AGCT 5 cut(s) 211, 320, 503, 553, 617
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 8 cut(s) 98, 347, 360, 535, 678, 691, 710, 723
AlwNI CAGNNNCTG 1 cut(s) 92
Aor51HI AGCGCT 1 cut(s) 259
AoxI GGCC 4 cut(s) 306, 384, 424, 621
ApeKI GCWGC 3 cut(s) 317, 365, 865
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 2 cut(s) 113, 444
AspA2I CCTAGG 1 cut(s) 73
AspLEI GCGC 2 cut(s) 260, 346
AspS9I GGNCC 2 cut(s) 384, 425
AsuC2I CCSGG 4 cut(s) 362, 428, 464, 708
AsuHPI GGTGA 3 cut(s) 491, 497, 602
AvrII CCTAGG 1 cut(s) 73
BamHI GGATCC 3 cut(s) 352, 683, 715
BanI GGYRCC 2 cut(s) 343, 643
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 3 cut(s) 329, 352, 877
BccI CCATC 5 cut(s) 41, 164, 417, 634, 819
BceAI ACGGC 2 cut(s) 250, 439
BcnI CCSGG 4 cut(s) 362, 428, 464, 708
BcoDI GTCTC 1 cut(s) 304
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 3 cut(s) 14, 74, 678
BfoI RGCGCY 2 cut(s) 261, 347
BglI GCCNNNNNGGC 1 cut(s) 75
BisI GCNGC 4 cut(s) 318, 366, 387, 866
BlnI CCTAGG 1 cut(s) 73
BlsI GCNGC 4 cut(s) 319, 367, 388, 867
Bme1390I CCNGG 4 cut(s) 362, 428, 464, 708
BmgT120I GGNCC 2 cut(s) 384, 425
BmiI GGNNCC 5 cut(s) 345, 354, 645, 685, 717
BmrFI CCNGG 4 cut(s) 362, 428, 464, 708
BmsI GCATC 2 cut(s) 583, 763
BpiI GAAGAC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 4 cut(s) 362, 428, 464, 708
BsaHI GRCGYC 2 cut(s) 132, 344
BsaJI CCNNGG 2 cut(s) 73, 303
BsaWI WCCGGW 2 cut(s) 113, 444
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 220, 585, 605, 725
Bse118I RCCGGY 2 cut(s) 113, 444
Bse1I ACTGG 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 73, 303
BseGI GGATG 4 cut(s) 80, 574, 778, 830
BseLI CCNNNNNNNGG 5 cut(s) 113, 220, 585, 605, 725
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 1 cut(s) 440
BseRI GAGGAG 1 cut(s) 875
BseXI GCAGC 3 cut(s) 329, 352, 877
BseYI CCCAGC 1 cut(s) 488
Bsh1285I CGRYCG 2 cut(s) 58, 67
BshFI GGCC 4 cut(s) 308, 386, 426, 623
BshNI GGYRCC 2 cut(s) 343, 643
BshTI ACCGGT 2 cut(s) 113, 444
BsiEI CGRYCG 2 cut(s) 58, 67
BsiSI CCGG 7 cut(s) 114, 347, 362, 428, 445, 463, 707
BslFI GGGAC 2 cut(s) 74, 391
BslI CCNNNNNNNGG 5 cut(s) 113, 220, 585, 605, 725
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 2 cut(s) 74, 391
BsnI GGCC 4 cut(s) 308, 386, 426, 623
Bsp143I GATC 6 cut(s) 103, 352, 527, 683, 715, 924
BspACI CCGC 7 cut(s) 67, 147, 189, 237, 387, 658, 764
BspANI GGCC 4 cut(s) 308, 386, 426, 623
BspCNI CTCAG 1 cut(s) 523
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 5 cut(s) 345, 354, 645, 685, 717
BspPI GGATC 8 cut(s) 98, 347, 360, 535, 678, 691, 710, 723
BspT107I GGYRCC 2 cut(s) 343, 643
BsrFI RCCGGY 2 cut(s) 113, 444
BsrI ACTGG 1 cut(s) 440
BssAI RCCGGY 2 cut(s) 113, 444
BssECI CCNNGG 2 cut(s) 73, 303
BssMI GATC 6 cut(s) 103, 352, 527, 683, 715, 924
BssNAI GTATAC 1 cut(s) 46
BssNI GRCGYC 2 cut(s) 132, 344
BssT1I CCWWGG 1 cut(s) 73
Bst1107I GTATAC 1 cut(s) 46
Bst4CI ACNGT 3 cut(s) 56, 88, 803
Bst6I CTCTTC 2 cut(s) 222, 812
BstACI GRCGYC 2 cut(s) 132, 344
BstC8I GCNNGC 3 cut(s) 97, 342, 648
BstDEI CTNAG 2 cut(s) 531, 632
BstF5I GGATG 4 cut(s) 80, 574, 778, 830
BstH2I RGCGCY 2 cut(s) 261, 347
BstHHI GCGC 2 cut(s) 260, 346
BstKTI GATC 6 cut(s) 106, 355, 530, 686, 718, 927
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 6 cut(s) 103, 352, 527, 683, 715, 924
BstMCI CGRYCG 2 cut(s) 58, 67
BstMWI GCNNNNNNNGC 4 cut(s) 75, 255, 314, 500
BstNSI RCATGY 2 cut(s) 99, 872
BstSCI CCNGG 4 cut(s) 360, 426, 462, 706
BstV1I GCAGC 3 cut(s) 329, 352, 877
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 4 cut(s) 352, 527, 683, 715
BstYI RGATCY 4 cut(s) 352, 527, 683, 715
BstZ17I GTATAC 1 cut(s) 46
BsuRI GGCC 4 cut(s) 308, 386, 426, 623
BtsCI GGATG 4 cut(s) 80, 574, 778, 830
BtsIMutI CAGTG 1 cut(s) 808
Cac8I GCNNGC 3 cut(s) 97, 342, 648
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 936
CfoI GCGC 2 cut(s) 260, 346
Cfr10I RCCGGY 2 cut(s) 113, 444
Cfr13I GGNCC 2 cut(s) 384, 425
CspAI ACCGGT 2 cut(s) 113, 444
CviAII CATG 6 cut(s) 20, 96, 511, 869, 913, 937
DdeI CTNAG 2 cut(s) 531, 632
DinI GGCGCC 1 cut(s) 345
DpnI GATC 6 cut(s) 105, 354, 529, 685, 717, 926
DpnII GATC 6 cut(s) 103, 352, 527, 683, 715, 924
EaeI YGGCCR 1 cut(s) 621
Eam1104I CTCTTC 2 cut(s) 222, 812
EarI CTCTTC 2 cut(s) 222, 812
Eco130I CCWWGG 1 cut(s) 73
Eco47III AGCGCT 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 73
EcoT22I ATGCAT 1 cut(s) 101
EgeI GGCGCC 1 cut(s) 345
EheI GGCGCC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 6 cut(s) 23, 99, 514, 872, 916, 940
FaqI GGGAC 2 cut(s) 74, 391
FatI CATG 6 cut(s) 19, 95, 510, 868, 912, 936
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 4 cut(s) 318, 366, 387, 866
FokI GGATG 4 cut(s) 67, 561, 785, 837
Fsp4HI GCNGC 4 cut(s) 318, 366, 387, 866
FspBI CTAG 3 cut(s) 14, 74, 678
GlaI GCGC 2 cut(s) 259, 345
GluI GCNGC 4 cut(s) 318, 366, 387, 866
GsaI CCCAGC 1 cut(s) 492
HaeII RGCGCY 2 cut(s) 261, 347
HaeIII GGCC 4 cut(s) 308, 386, 426, 623
HapII CCGG 7 cut(s) 114, 347, 362, 428, 445, 463, 707
HhaI GCGC 2 cut(s) 260, 346
Hin1I GRCGYC 2 cut(s) 132, 344
Hin1II CATG 6 cut(s) 23, 99, 514, 872, 916, 940
Hin6I GCGC 2 cut(s) 258, 344
HinP1I GCGC 2 cut(s) 258, 344
HinfI GANTC 5 cut(s) 60, 197, 377, 404, 628
HpaII CCGG 7 cut(s) 114, 347, 362, 428, 445, 463, 707
HphI GGTGA 3 cut(s) 491, 497, 602
Hpy166II GTNNAC 2 cut(s) 46, 226
Hpy188I TCNGA 5 cut(s) 304, 532, 688, 720, 850
Hpy188III TCNNGA 2 cut(s) 919, 937
Hpy8I GTNNAC 2 cut(s) 46, 226
Hpy99I CGWCG 2 cut(s) 11, 137
HpyAV CCTTC 2 cut(s) 763, 850
HpyCH4III ACNGT 3 cut(s) 56, 88, 803
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 95, 99, 164, 317, 452, 574
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 255, 314, 500
HpyF3I CTNAG 2 cut(s) 531, 632
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 2 cut(s) 132, 344
Hsp92II CATG 6 cut(s) 23, 99, 514, 872, 916, 940
HspAI GCGC 2 cut(s) 258, 344
KasI GGCGCC 1 cut(s) 343
Kzo9I GATC 6 cut(s) 103, 352, 527, 683, 715, 924
LmnI GCTCC 2 cut(s) 216, 862
Lsp1109I GCAGC 3 cut(s) 329, 352, 877
LweI GCATC 2 cut(s) 583, 763
MaeI CTAG 3 cut(s) 14, 74, 678
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 896, 907
MalI GATC 6 cut(s) 105, 354, 529, 685, 717, 926
MboI GATC 6 cut(s) 103, 352, 527, 683, 715, 924
MboII GAAGA 7 cut(s) 209, 386, 443, 605, 826, 829, 934
MflI RGATCY 4 cut(s) 352, 527, 683, 715
MluCI AATT 1 cut(s) 940
Mly113I GGCGCC 1 cut(s) 344
MlyI GAGTC 3 cut(s) 206, 413, 637
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 8 cut(s) 80, 225, 296, 298, 343, 799, 835, 853
Mph1103I ATGCAT 1 cut(s) 101
MseI TTAA 2 cut(s) 549, 702
MslI CAYNNNNRTG 2 cut(s) 237, 394
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 7 cut(s) 114, 347, 362, 428, 445, 463, 707
MspR9I CCNGG 4 cut(s) 362, 428, 464, 708
MwoI GCNNNNNNNGC 4 cut(s) 75, 255, 314, 500
NarI GGCGCC 1 cut(s) 344
NciI CCSGG 4 cut(s) 362, 428, 464, 708
NdeII GATC 6 cut(s) 103, 352, 527, 683, 715, 924
NlaIII CATG 6 cut(s) 23, 99, 514, 872, 916, 940
NlaIV GGNNCC 5 cut(s) 345, 354, 645, 685, 717
NmeAIII GCCGAG 1 cut(s) 649
NmuCI GTSAC 2 cut(s) 896, 907
NsiI ATGCAT 1 cut(s) 101
NspI RCATGY 2 cut(s) 99, 872
OliI CACNNNNGTG 1 cut(s) 237
PaeI GCATGC 1 cut(s) 99
PagI TCATGA 1 cut(s) 936
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 2 cut(s) 60, 377
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 2 cut(s) 113, 444
PkrI GCNGC 4 cut(s) 319, 367, 388, 867
PleI GAGTC 3 cut(s) 205, 412, 636
PluTI GGCGCC 1 cut(s) 347
PpsI GAGTC 3 cut(s) 205, 412, 636
PspFI CCCAGC 1 cut(s) 488
PspN4I GGNNCC 5 cut(s) 345, 354, 645, 685, 717
PspPI GGNCC 2 cut(s) 384, 425
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 4 cut(s) 352, 527, 683, 715
PsyI GACNNNGTC 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 320
RseI CAYNNNNRTG 2 cut(s) 237, 394
SaqAI TTAA 2 cut(s) 549, 702
SatI GCNGC 4 cut(s) 318, 366, 387, 866
Sau3AI GATC 6 cut(s) 103, 352, 527, 683, 715, 924
Sau96I GGNCC 2 cut(s) 384, 425
SchI GAGTC 3 cut(s) 206, 413, 637
ScrFI CCNGG 4 cut(s) 362, 428, 464, 708
SfaNI GCATC 2 cut(s) 583, 763
SfoI GGCGCC 1 cut(s) 345
SmiMI CAYNNNNRTG 2 cut(s) 237, 394
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SpeI ACTAGT 1 cut(s) 677
SphI GCATGC 1 cut(s) 99
Sse9I AATT 1 cut(s) 940
SsiI CCGC 7 cut(s) 67, 147, 189, 237, 387, 658, 764
SspDI GGCGCC 1 cut(s) 343
SspMI CTAG 3 cut(s) 14, 74, 678
StyD4I CCNGG 4 cut(s) 360, 426, 462, 706
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 3 cut(s) 56, 88, 803
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 58, 63
TasI AATT 1 cut(s) 940
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 2 cut(s) 60, 377
Tru1I TTAA 2 cut(s) 549, 702
Tru9I TTAA 2 cut(s) 549, 702
TscAI CASTG 1 cut(s) 808
TseFI GTSAC 2 cut(s) 896, 907
TseI GCWGC 3 cut(s) 317, 365, 865
Tsp45I GTSAC 2 cut(s) 896, 907
TspDTI ATGAA 2 cut(s) 387, 925
TspRI CASTG 1 cut(s) 808
Tth111I GACNNNGTC 1 cut(s) 134
XceI RCATGY 2 cut(s) 99, 872
XmaJI CCTAGG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 3 cut(s) 14, 74, 678
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.