pycom10g07450

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000397
Physical Location & Seq
Forward (+)
8806712 .. 8807632
921 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g07450.1

Sequence Viewer

Length: 921 bp
ATGGACTCTAAACCCTCCAGCGAAGTATCATTCGAGTTCCCGACTGTTTTCCGAATCTATAACGACGGCCGAACCGAGAGACTCAAGGGCATCGAAACCGTCCCACCCTCCACCGACCTGACAACCGGGGTCCAATCCAAAGACATCGTCCTCTCGCCACAATCCGGGCTCTCTGCCCGCGTCTTCCTCCCCAAGCTCCCCGACCCGACCCGCAAACTCCCTCTCCTTATTTTCATCCACGGCGACACTTTCGTCATCGAGTCCCTCTACTCTCCTCTCTACCATAACCACGTCGGGTTACTGGATTCCGAAGCCAACGTCGTGGCTTTGTCGGTGCACTACAGGCGTGCCCCGGAGCACCCACTCCTCGTTGCTTTTGAAGACTCCTGGGATGCAGTCCAATGGGCCGCGACCCATTCCTCTGGTAACGGGCTTGAGGCCTGGCTCAACGATCACGTTGACTTTGACCGTGTTTTTATGGGTGGTGACAGCGCTGGCGCCACTCTGACGCACAACGTGGTGCGGCAGGCCGGGCTTGATGGGTTGAGCGGGACAAGGATAGTGGGGATGATTTTGTTTCACCCCTACTTCATGGATGACGAGCCTGATAAGTTGTTGGAGGTTATTTATCCGACATGTGGTGGGTCGGATGACCCGAGGGTGAGGCCGGGCAATTTGAAGATCGATCCGAAATTGGGAGAGATTGGGTGCGGGAGGGTGTTGGTTTTTGTGGCTGAGAAGGATTTCTTGAGGGACAAGGGTTGGGCATACTACGAGGCGTTGAAGAAGAGTGGGTATGGTGGGGTGGTTGAGATTGTGGAGAGTGAAGGGGAGGACCACGTGTTTCATTTGTTCAACCCAAGTTGTGACAGTTCTGTGGACTTGGTGAAAAAGGTGGTTTCTTTCGTAAATCAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0009056 GO:0016787 GO:0016788 GO:0044424 GO:0044444 GO:0044464 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

307

Amino Acids

33.82

Weight (kDa)

5.05

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 76 - 283 2.7e-37 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 497
AccBSI CCGCTC 1 cut(s) 549
AccII CGCG 2 cut(s) 180, 410
AciI CCGC 6 cut(s) 178, 211, 408, 523, 549, 711
AclWI GGATC 1 cut(s) 680
AcoI YGGCCR 1 cut(s) 67
AcvI CACGTG 1 cut(s) 841
AcyI GRCGYC 1 cut(s) 498
AdeI CACNNNGTG 1 cut(s) 517
AfeI AGCGCT 1 cut(s) 493
AfiI CCNNNNNNNGG 3 cut(s) 164, 638, 695
AflIII ACRYGT 2 cut(s) 635, 840
AgsI TTSAA 4 cut(s) 380, 679, 784, 856
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 2 cut(s) 386, 440
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw21I GWGCWC 2 cut(s) 339, 360
Alw26I GTCTC 1 cut(s) 73
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 1 cut(s) 680
Ama87I CYCGRG 1 cut(s) 655
Aor51HI AGCGCT 1 cut(s) 493
AoxI GGCC 5 cut(s) 67, 405, 438, 528, 665
ApaLI GTGCAC 1 cut(s) 335
ArsI GACNNNNNNTTYG 4 cut(s) 132, 164, 303, 335
Asp700I GAANNNNTTC 1 cut(s) 743
AspLEI GCGC 2 cut(s) 494, 500
AspS9I GGNCC 3 cut(s) 130, 405, 835
AsuC2I CCSGG 5 cut(s) 127, 166, 353, 532, 669
AsuHPI GGTGA 4 cut(s) 497, 572, 673, 898
AvaI CYCGRG 1 cut(s) 655
AvaII GGWCC 2 cut(s) 130, 835
BaeGI GKGCMC 2 cut(s) 339, 352
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 171
BbrPI CACGTG 1 cut(s) 841
BbsI GAAGAC 2 cut(s) 175, 387
Bbv12I GWGCWC 2 cut(s) 339, 360
BccI CCATC 1 cut(s) 533
BceAI ACGGC 2 cut(s) 82, 256
BciT130I CCWGG 2 cut(s) 388, 442
BcnI CCSGG 5 cut(s) 127, 166, 353, 532, 669
BcoDI GTCTC 1 cut(s) 73
BfmI CTRYAG 1 cut(s) 340
BfoI RGCGCY 2 cut(s) 495, 501
BisI GCNGC 2 cut(s) 408, 524
BlsI GCNGC 2 cut(s) 409, 525
Bme1390I CCNGG 7 cut(s) 127, 166, 353, 388, 442, 532, 669
Bme18I GGWCC 2 cut(s) 130, 835
BmeT110I CYCGRG 1 cut(s) 655
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 130, 405, 835
BmiI GGNNCC 2 cut(s) 131, 499
BmrFI CCNGG 7 cut(s) 127, 166, 353, 388, 442, 532, 669
BmsI GCATC 2 cut(s) 99, 382
BpiI GAAGAC 2 cut(s) 175, 387
BpuEI CTTGAG 3 cut(s) 68, 455, 769
BpuMI CCSGG 5 cut(s) 127, 166, 353, 532, 669
Bsa29I ATCGAT 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 841
BsaHI GRCGYC 1 cut(s) 498
BsaJI CCNNGG 5 cut(s) 126, 238, 351, 387, 656
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 638, 695
Bse1I ACTGG 1 cut(s) 306
BseBI CCWGG 2 cut(s) 388, 442
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 5 cut(s) 126, 238, 351, 387, 656
BseGI GGATG 5 cut(s) 234, 397, 573, 601, 655
BseLI CCNNNNNNNGG 3 cut(s) 164, 638, 695
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 2 cut(s) 264, 356
BseSI GKGCMC 2 cut(s) 339, 352
BseX3I CGGCCG 1 cut(s) 67
Bsh1236I CGCG 2 cut(s) 180, 410
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 69, 407, 440, 530, 667
BshNI GGYRCC 1 cut(s) 497
BshVI ATCGAT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 2 cut(s) 339, 360
BsiHKCI CYCGRG 1 cut(s) 655
BsiSI CCGG 5 cut(s) 126, 165, 353, 531, 668
BslFI GGGAC 4 cut(s) 86, 247, 565, 767
BslI CCNNNNNNNGG 3 cut(s) 164, 638, 695
BsmAI GTCTC 1 cut(s) 73
BsmFI GGGAC 4 cut(s) 86, 247, 565, 767
BsnI GGCC 5 cut(s) 69, 407, 440, 530, 667
BsoBI CYCGRG 1 cut(s) 655
Bsp1286I GDGCHC 4 cut(s) 171, 339, 352, 360
Bsp143I GATC 3 cut(s) 451, 681, 685
BspACI CCGC 6 cut(s) 178, 211, 408, 523, 549, 711
BspANI GGCC 5 cut(s) 69, 407, 440, 530, 667
BspCNI CTCAG 1 cut(s) 727
BspDI ATCGAT 1 cut(s) 684
BspFNI CGCG 2 cut(s) 180, 410
BspLI GGNNCC 2 cut(s) 131, 499
BspPI GGATC 1 cut(s) 680
BspT107I GGYRCC 1 cut(s) 497
BsrBI CCGCTC 1 cut(s) 549
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 5 cut(s) 126, 238, 351, 387, 656
BssMI GATC 3 cut(s) 451, 681, 685
BssNI GRCGYC 1 cut(s) 498
Bst2UI CCWGG 2 cut(s) 388, 442
Bst4CI ACNGT 4 cut(s) 46, 100, 470, 872
Bst6I CTCTTC 1 cut(s) 782
BstACI GRCGYC 1 cut(s) 498
BstBAI YACGTR 1 cut(s) 841
BstC8I GCNNGC 4 cut(s) 178, 348, 496, 528
BstDEI CTNAG 1 cut(s) 735
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 5 cut(s) 234, 397, 573, 601, 655
BstFNI CGCG 2 cut(s) 180, 410
BstH2I RGCGCY 2 cut(s) 495, 501
BstHHI GCGC 2 cut(s) 494, 500
BstKTI GATC 3 cut(s) 454, 684, 688
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 3 cut(s) 451, 681, 685
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 2 cut(s) 343, 532
BstNI CCWGG 2 cut(s) 388, 442
BstNSI RCATGY 1 cut(s) 639
BstSCI CCNGG 7 cut(s) 125, 164, 351, 386, 440, 530, 667
BstSFI CTRYAG 1 cut(s) 340
BstSLI GKGCMC 2 cut(s) 339, 352
BstUI CGCG 2 cut(s) 180, 410
BstV2I GAAGAC 2 cut(s) 175, 387
BstXI CCANNNNNNTGG 2 cut(s) 322, 422
BstZI CGGCCG 1 cut(s) 67
Bsu15I ATCGAT 1 cut(s) 684
BsuRI GGCC 5 cut(s) 69, 407, 440, 530, 667
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 5 cut(s) 234, 397, 573, 601, 655
Cac8I GCNNGC 4 cut(s) 178, 348, 496, 528
CfoI GCGC 2 cut(s) 494, 500
Cfr13I GGNCC 3 cut(s) 130, 405, 835
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 2 cut(s) 169, 517
CspCI CAANNNNNGTGG 2 cut(s) 543, 578
CviAII CATG 2 cut(s) 592, 636
DdeI CTNAG 1 cut(s) 735
DinI GGCGCC 1 cut(s) 499
DpnI GATC 3 cut(s) 453, 683, 687
DpnII GATC 3 cut(s) 451, 681, 685
DraIII CACNNNGTG 1 cut(s) 517
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 67
EagI CGGCCG 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 782
EarI CTCTTC 1 cut(s) 782
EclXI CGGCCG 1 cut(s) 67
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 2 cut(s) 130, 835
Eco47III AGCGCT 1 cut(s) 493
Eco52I CGGCCG 1 cut(s) 67
Eco72I CACGTG 1 cut(s) 841
Eco88I CYCGRG 1 cut(s) 655
EcoRII CCWGG 2 cut(s) 386, 440
EcoT38I GRGCYC 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 499
EheI GGCGCC 1 cut(s) 499
FaeI CATG 2 cut(s) 595, 639
FaiI YATR 7 cut(s) 60, 285, 479, 593, 637, 769, 798
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FaqI GGGAC 4 cut(s) 86, 247, 565, 767
FatI CATG 2 cut(s) 591, 635
FauI CCCGC 4 cut(s) 185, 218, 542, 704
Fnu4HI GCNGC 2 cut(s) 408, 524
FokI GGATG 5 cut(s) 221, 404, 580, 608, 662
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 2 cut(s) 408, 524
GlaI GCGC 2 cut(s) 493, 499
GluI GCNGC 2 cut(s) 408, 524
HaeII RGCGCY 2 cut(s) 495, 501
HaeIII GGCC 5 cut(s) 69, 407, 440, 530, 667
HapII CCGG 5 cut(s) 126, 165, 353, 531, 668
HgaI GACGC 2 cut(s) 169, 517
HhaI GCGC 2 cut(s) 494, 500
Hin1I GRCGYC 1 cut(s) 498
Hin1II CATG 2 cut(s) 595, 639
Hin6I GCGC 2 cut(s) 492, 498
HinP1I GCGC 2 cut(s) 492, 498
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 6 cut(s) 5, 54, 81, 260, 305, 383
HpaII CCGG 5 cut(s) 126, 165, 353, 531, 668
HphI GGTGA 4 cut(s) 497, 572, 673, 898
Hpy166II GTNNAC 3 cut(s) 337, 460, 880
Hpy188I TCNGA 6 cut(s) 53, 310, 507, 633, 649, 690
Hpy188III TCNNGA 3 cut(s) 40, 748, 914
Hpy8I GTNNAC 3 cut(s) 337, 460, 880
Hpy99I CGWCG 3 cut(s) 68, 296, 323
HpyAV CCTTC 2 cut(s) 733, 821
HpyCH4III ACNGT 4 cut(s) 46, 100, 470, 872
HpyCH4IV ACGT 5 cut(s) 291, 318, 456, 516, 840
HpyCH4V TGCA 2 cut(s) 337, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 343, 532
HpyF3I CTNAG 1 cut(s) 735
HpySE526I ACGT 5 cut(s) 291, 318, 456, 516, 840
Hsp92I GRCGYC 1 cut(s) 498
Hsp92II CATG 2 cut(s) 595, 639
HspAI GCGC 2 cut(s) 492, 498
KasI GGCGCC 1 cut(s) 497
Kzo9I GATC 3 cut(s) 451, 681, 685
LmnI GCTCC 2 cut(s) 201, 355
LweI GCATC 2 cut(s) 99, 382
MaeII ACGT 5 cut(s) 291, 318, 456, 516, 840
MaeIII GTNAC 4 cut(s) 297, 425, 485, 866
MalI GATC 3 cut(s) 453, 683, 687
MbiI CCGCTC 1 cut(s) 549
MboI GATC 3 cut(s) 451, 681, 685
MboII GAAGA 5 cut(s) 175, 392, 691, 796, 799
MhlI GDGCHC 4 cut(s) 171, 339, 352, 360
MluCI AATT 2 cut(s) 673, 692
Mly113I GGCGCC 1 cut(s) 498
MlyI GAGTC 3 cut(s) 75, 269, 377
MmeI TCCRAC 3 cut(s) 597, 627, 656
MroXI GAANNNNTTC 1 cut(s) 743
MspI CCGG 5 cut(s) 126, 165, 353, 531, 668
MspR9I CCNGG 7 cut(s) 127, 166, 353, 388, 442, 532, 669
MvaI CCWGG 2 cut(s) 388, 442
MvnI CGCG 2 cut(s) 180, 410
MwoI GCNNNNNNNGC 2 cut(s) 343, 532
NarI GGCGCC 1 cut(s) 498
NciI CCSGG 5 cut(s) 127, 166, 353, 532, 669
NdeII GATC 3 cut(s) 451, 681, 685
NlaIII CATG 2 cut(s) 595, 639
NlaIV GGNNCC 2 cut(s) 131, 499
NmuCI GTSAC 2 cut(s) 485, 866
NspI RCATGY 1 cut(s) 639
PceI AGGCCT 1 cut(s) 440
PciI ACATGT 1 cut(s) 635
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 653
PdmI GAANNNNTTC 1 cut(s) 743
PfeI GAWTC 2 cut(s) 54, 305
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 2 cut(s) 409, 525
PleI GAGTC 3 cut(s) 75, 268, 377
PluTI GGCGCC 1 cut(s) 501
PmaCI CACGTG 1 cut(s) 841
PmlI CACGTG 1 cut(s) 841
PpsI GAGTC 3 cut(s) 75, 268, 377
Ppu21I YACGTR 1 cut(s) 841
PscI ACATGT 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 386, 440
PspCI CACGTG 1 cut(s) 841
PspGI CCWGG 2 cut(s) 386, 440
PspN4I GGNNCC 2 cut(s) 131, 499
PspPI GGNCC 3 cut(s) 130, 405, 835
PsyI GACNNNGTC 1 cut(s) 146
SatI GCNGC 2 cut(s) 408, 524
Sau3AI GATC 3 cut(s) 451, 681, 685
Sau96I GGNCC 3 cut(s) 130, 405, 835
SchI GAGTC 3 cut(s) 75, 269, 377
ScrFI CCNGG 7 cut(s) 127, 166, 353, 388, 442, 532, 669
SduI GDGCHC 4 cut(s) 171, 339, 352, 360
SetI ASST 9 cut(s) 120, 198, 294, 321, 459, 519, 624, 843, 897
SfaNI GCATC 2 cut(s) 99, 382
SfcI CTRYAG 1 cut(s) 340
SfoI GGCGCC 1 cut(s) 499
SinI GGWCC 2 cut(s) 130, 835
SmlI CTYRAG 3 cut(s) 83, 434, 748
SmoI CTYRAG 3 cut(s) 83, 434, 748
Sse9I AATT 2 cut(s) 673, 692
SseBI AGGCCT 1 cut(s) 440
SsiI CCGC 6 cut(s) 178, 211, 408, 523, 549, 711
SspDI GGCGCC 1 cut(s) 497
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 7 cut(s) 125, 164, 351, 386, 440, 530, 667
TaaI ACNGT 4 cut(s) 46, 100, 470, 872
TaiI ACGT 5 cut(s) 294, 321, 459, 519, 843
TaqI TCGA 4 cut(s) 33, 93, 258, 684
TasI AATT 2 cut(s) 673, 692
TauI GCSGC 2 cut(s) 410, 526
TfiI GAWTC 2 cut(s) 54, 305
TseFI GTSAC 2 cut(s) 485, 866
Tsp45I GTSAC 2 cut(s) 485, 866
TspDTI ATGAA 3 cut(s) 223, 580, 836
Tth111I GACNNNGTC 1 cut(s) 146
VneI GTGCAC 1 cut(s) 335
VpaK11BI GGWCC 2 cut(s) 130, 835
XceI RCATGY 1 cut(s) 639
XmnI GAANNNNTTC 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.