MD05G1191000.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000397
Physical Location & Seq
Forward (+)
31975923 .. 31976858
936 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1191000.v1.1.491

Sequence Viewer

Length: 936 bp
ATGAGCAACGAAGAAGTAGCCTATGATCTCACTCCGTTGATCAAAGTATACAAAGACGGTCGAGTCGAGAGACTCCAAGGCACAGCCACAGTTCCTCCGTCAACAGTCCAATCCAAAGACATTGTCATCTCCCAACAACCACCCGTTTCCGCAAGGCTTTACCTCCCCAAATCCACCGAAACCAAACTCCCTCTTCTCGTTTACTTTCACGGCGGAGGCTTCTGCATTAATAGCGCCTTTTCTCCCAAGTATCATAATTACCTCAACTCCTTAGTCTCCGAAGCCAAAGTTGTGGCCGTCTCTGTTGAGTACAGGCTTGCCCCGGAGAACCCTCTCCCAGCCGCCTACGACGATTCGTGGGCTGCTCTCAAATGGGTCGCTTCCCATTTTGATGGAACGCGAAATGTTGATGAAGAAGAAGACGATTGGCTAAGTAGCTACGCGGATTCGCAGCGTGTGTTTTTCTCTGGAGACAGCTGTGGAGCTACTTTAGCGCACTACATGGGAGTGAGAATAGGCTCTGAGGGGCTGGTTGGTGTTAAGCTGATTGGGGTTGTGTTGGTCGATCCATATTTTTGGGGGGAAGAGCTAATTGGGGGAGAGATCACTCTCCCTGCAGCTGAAAGGGAGTTTATTGCTGCATTGTGGCGTTTTACCTACCCATCGACTATTGGATCAGATGACCCCCTTATCAACCCTCAAAAGGATCCGAAATTTGGGGAATTGGGGTGTGGAAAAGTACTGGTTTTTGTGGCTGAGTTAGATGTGTTGAAATACAGGGGATTGTACTATGGGGAGGCGCTGAGAAAGAGTGGATGGAATGGAGTTGTGGAGGTGATAGAAACAAAGGGGAAGGACCATAATTACCATTTGTCCGATCCTACCTGTGAGAATGCTGTGGCCATGCAGAAAAAGATAGTCTCGTTCTTGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

312

Amino Acids

34.38

Weight (kDa)

5.08

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 53 - 171 8.3e-13 BD-FAE
COesterase PF00135 54 - 108 5.3e-07 Carboxylesterase family
Abhydrolase_3 PF07859 66 - 290 1.2e-47 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 48
AccII CGCG 2 cut(s) 400, 443
AciI CCGC 4 cut(s) 150, 213, 342, 443
AclWI GGATC 5 cut(s) 560, 682, 701, 714, 872
AcoI YGGCCR 2 cut(s) 294, 900
AcsI RAATTY 1 cut(s) 713
AfaI GTAC 3 cut(s) 311, 741, 788
AfiI CCNNNNNNNGG 2 cut(s) 703, 716
AgsI TTSAA 2 cut(s) 772, 931
AjuI GAANNNNNNNTTGG 2 cut(s) 576, 608
AluBI AGCT 6 cut(s) 438, 477, 485, 544, 589, 620
AluI AGCT 6 cut(s) 438, 477, 485, 544, 589, 620
Alw26I GTCTC 5 cut(s) 64, 280, 304, 465, 925
AlwI GGATC 5 cut(s) 560, 682, 701, 714, 872
AoxI GGCC 2 cut(s) 294, 900
ApeKI GCWGC 4 cut(s) 362, 451, 617, 638
ApoI RAATTY 1 cut(s) 713
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AseI ATTAAT 1 cut(s) 228
AspLEI GCGC 3 cut(s) 236, 496, 802
AspS9I GGNCC 1 cut(s) 856
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 847
AvaII GGWCC 1 cut(s) 856
BalI TGGCCA 1 cut(s) 902
BamHI GGATCC 1 cut(s) 706
BbsI GAAGAC 1 cut(s) 426
BbvI GCAGC 4 cut(s) 349, 463, 625, 629
BccI CCATC 3 cut(s) 386, 670, 810
BceAI ACGGC 2 cut(s) 226, 281
BclI TGATCA 1 cut(s) 39
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 5 cut(s) 64, 280, 304, 465, 925
BfaI CTAG 1 cut(s) 934
BfmI CTRYAG 1 cut(s) 615
BfoI RGCGCY 2 cut(s) 237, 803
BisI GCNGC 5 cut(s) 342, 363, 452, 618, 639
BlsI GCNGC 5 cut(s) 343, 364, 453, 619, 640
BmcAI AGTACT 1 cut(s) 741
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 1 cut(s) 856
BmgT120I GGNCC 1 cut(s) 856
BmiI GGNNCC 1 cut(s) 708
BmrFI CCNGG 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 76, 321
BsaXI ACNNNNNCTCC 4 cut(s) 79, 109, 251, 281
Bsc4I CCNNNNNNNGG 2 cut(s) 703, 716
Bse1I ACTGG 1 cut(s) 747
BseDI CCNNGG 2 cut(s) 76, 321
BseGI GGATG 1 cut(s) 821
BseLI CCNNNNNNNGG 2 cut(s) 703, 716
BseMII CTCAG 3 cut(s) 513, 747, 794
BseNI ACTGG 1 cut(s) 747
BseXI GCAGC 4 cut(s) 349, 463, 625, 629
BseYI CCCAGC 1 cut(s) 337
Bsh1236I CGCG 2 cut(s) 400, 443
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 2 cut(s) 296, 902
BsiEI CGRYCG 1 cut(s) 61
BsiSI CCGG 1 cut(s) 323
BslI CCNNNNNNNGG 2 cut(s) 703, 716
BsmAI GTCTC 5 cut(s) 64, 280, 304, 465, 925
BsmBI CGTCTC 1 cut(s) 304
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 2 cut(s) 296, 902
Bsp143I GATC 7 cut(s) 25, 39, 565, 603, 674, 706, 877
BspACI CCGC 4 cut(s) 150, 213, 342, 443
BspANI GGCC 2 cut(s) 296, 902
BspCNI CTCAG 3 cut(s) 514, 748, 795
BspFNI CGCG 2 cut(s) 400, 443
BspLI GGNNCC 1 cut(s) 708
BspMAI CTGCAG 1 cut(s) 619
BspPI GGATC 5 cut(s) 560, 682, 701, 714, 872
BspQI GCTCTTC 1 cut(s) 579
BsrI ACTGG 1 cut(s) 747
BssECI CCNNGG 2 cut(s) 76, 321
BssMI GATC 7 cut(s) 25, 39, 565, 603, 674, 706, 877
BssNAI GTATAC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 76
Bst1107I GTATAC 1 cut(s) 49
Bst4CI ACNGT 3 cut(s) 59, 91, 106
Bst6I CTCTTC 2 cut(s) 198, 579
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 5 cut(s) 271, 431, 522, 756, 803
BstF5I GGATG 1 cut(s) 821
BstFNI CGCG 2 cut(s) 400, 443
BstH2I RGCGCY 2 cut(s) 237, 803
BstHHI GCGC 3 cut(s) 236, 496, 802
BstKTI GATC 7 cut(s) 28, 42, 568, 606, 677, 709, 880
BstMAI GTCTC 5 cut(s) 64, 280, 304, 465, 925
BstMBI GATC 7 cut(s) 25, 39, 565, 603, 674, 706, 877
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 231, 491
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 615
BstUI CGCG 2 cut(s) 400, 443
BstV1I GCAGC 4 cut(s) 349, 463, 625, 629
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 1 cut(s) 706
BstXI CCANNNNNNTGG 3 cut(s) 292, 392, 576
BstYI RGATCY 1 cut(s) 706
BstZ17I GTATAC 1 cut(s) 49
BsuRI GGCC 2 cut(s) 296, 902
BtsCI GGATG 1 cut(s) 821
Cac8I GCNNGC 1 cut(s) 318
CfoI GCGC 3 cut(s) 236, 496, 802
Cfr13I GGNCC 1 cut(s) 856
Csp6I GTAC 3 cut(s) 310, 740, 787
CviAII CATG 2 cut(s) 502, 904
CviQI GTAC 3 cut(s) 310, 740, 787
DdeI CTNAG 5 cut(s) 271, 431, 522, 756, 803
DpnI GATC 7 cut(s) 27, 41, 567, 605, 676, 708, 879
DpnII GATC 7 cut(s) 25, 39, 565, 603, 674, 706, 877
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 2 cut(s) 294, 900
Eam1104I CTCTTC 2 cut(s) 198, 579
EarI CTCTTC 2 cut(s) 198, 579
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 856
EcoT14I CCWWGG 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 2 cut(s) 505, 907
FaiI YATR 8 cut(s) 24, 49, 255, 503, 571, 792, 861, 905
FatI CATG 2 cut(s) 501, 903
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 5 cut(s) 342, 363, 452, 618, 639
FokI GGATG 1 cut(s) 828
Fsp4HI GCNGC 5 cut(s) 342, 363, 452, 618, 639
FspBI CTAG 1 cut(s) 934
GlaI GCGC 3 cut(s) 235, 495, 801
GluI GCNGC 5 cut(s) 342, 363, 452, 618, 639
GsaI CCCAGC 1 cut(s) 341
GsuI CTGGAG 1 cut(s) 489
HaeII RGCGCY 2 cut(s) 237, 803
HaeIII GGCC 2 cut(s) 296, 902
HapII CCGG 1 cut(s) 323
HhaI GCGC 3 cut(s) 236, 496, 802
Hin1II CATG 2 cut(s) 505, 907
Hin6I GCGC 3 cut(s) 234, 494, 800
HinP1I GCGC 3 cut(s) 234, 494, 800
HincII GTYRAC 1 cut(s) 102
HindII GTYRAC 1 cut(s) 102
HinfI GANTC 4 cut(s) 63, 72, 353, 446
HpaII CCGG 1 cut(s) 323
HphI GGTGA 1 cut(s) 847
Hpy166II GTNNAC 3 cut(s) 49, 102, 202
Hpy188I TCNGA 5 cut(s) 280, 523, 679, 711, 877
Hpy188III TCNNGA 3 cut(s) 67, 468, 928
Hpy8I GTNNAC 3 cut(s) 49, 102, 202
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 1 cut(s) 847
HpyCH4III ACNGT 3 cut(s) 59, 91, 106
HpyCH4V TGCA 4 cut(s) 225, 617, 641, 907
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 491
HpyF3I CTNAG 5 cut(s) 271, 431, 522, 756, 803
Hsp92II CATG 2 cut(s) 505, 907
HspAI GCGC 3 cut(s) 234, 494, 800
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 7 cut(s) 25, 39, 565, 603, 674, 706, 877
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 1 cut(s) 482
LpnPI CCDG 9 cut(s) 298, 336, 351, 453, 515, 627, 728, 763, 898
Lsp1109I GCAGC 4 cut(s) 349, 463, 625, 629
MaeI CTAG 1 cut(s) 934
MalI GATC 7 cut(s) 27, 41, 567, 605, 676, 708, 879
MboI GATC 7 cut(s) 25, 39, 565, 603, 674, 706, 877
MboII GAAGA 6 cut(s) 23, 185, 425, 428, 431, 596
MflI RGATCY 1 cut(s) 706
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 5 cut(s) 256, 591, 713, 722, 862
MluNI TGGCCA 1 cut(s) 902
MlyI GAGTC 2 cut(s) 66, 72
Mox20I TGGCCA 1 cut(s) 902
MscI TGGCCA 1 cut(s) 902
MseI TTAA 2 cut(s) 228, 540
MslI CAYNNNNRTG 2 cut(s) 390, 506
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 2 cut(s) 477, 620
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 1 cut(s) 323
Mva1269I GAATGC 1 cut(s) 898
MvnI CGCG 2 cut(s) 400, 443
MwoI GCNNNNNNNGC 2 cut(s) 231, 491
NciI CCSGG 1 cut(s) 323
NdeII GATC 7 cut(s) 25, 39, 565, 603, 674, 706, 877
NlaIII CATG 2 cut(s) 505, 907
NlaIV GGNNCC 1 cut(s) 708
PciSI GCTCTTC 1 cut(s) 579
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 898
PfeI GAWTC 2 cut(s) 353, 446
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 5 cut(s) 343, 364, 453, 619, 640
PleI GAGTC 2 cut(s) 66, 71
PpsI GAGTC 2 cut(s) 66, 71
PshBI ATTAAT 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 708
PspPI GGNCC 1 cut(s) 856
PstI CTGCAG 1 cut(s) 619
PsuI RGATCY 1 cut(s) 706
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 2 cut(s) 477, 620
RsaI GTAC 3 cut(s) 311, 741, 788
RsaNI GTAC 3 cut(s) 310, 740, 787
RseI CAYNNNNRTG 2 cut(s) 390, 506
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 2 cut(s) 228, 540
SatI GCNGC 5 cut(s) 342, 363, 452, 618, 639
Sau3AI GATC 7 cut(s) 25, 39, 565, 603, 674, 706, 877
Sau96I GGNCC 1 cut(s) 856
ScaI AGTACT 1 cut(s) 741
SchI GAGTC 2 cut(s) 66, 72
ScrFI CCNGG 1 cut(s) 323
SfcI CTRYAG 1 cut(s) 615
SinI GGWCC 1 cut(s) 856
SmiMI CAYNNNNRTG 2 cut(s) 390, 506
Sse9I AATT 5 cut(s) 256, 591, 713, 722, 862
SsiI CCGC 4 cut(s) 150, 213, 342, 443
SspMI CTAG 1 cut(s) 934
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 3 cut(s) 59, 91, 106
TaqI TCGA 4 cut(s) 61, 66, 564, 665
TasI AATT 5 cut(s) 256, 591, 713, 722, 862
TatI WGTACW 3 cut(s) 309, 739, 786
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 353, 446
Tru1I TTAA 2 cut(s) 228, 540
Tru9I TTAA 2 cut(s) 228, 540
TseI GCWGC 4 cut(s) 362, 451, 617, 638
TspDTI ATGAA 1 cut(s) 426
TspGWI ACGGA 2 cut(s) 24, 87
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 856
VspI ATTAAT 1 cut(s) 228
XapI RAATTY 1 cut(s) 713
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 1 cut(s) 934
ZrmI AGTACT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.