Rroxscaffold_178G00437350

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000178
OG0000397
Physical Location & Seq
Forward (+)
131520 .. 132434
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_178G00437350.1

Sequence Viewer

Length: 915 bp
ATGAGCGATGAGTTAGTCCATGACTTCTCTCCTCTCTTTAAAATCTACAAAGATGGTCGAATTGAGCGACTCATGGGCACAGATACAGTTCCTCCATCAGTTCATCCCCAAACTGGTGTCGAATCCAAAGACGTCATGATTTCAAACGAAACAGGATTATATGCTAGGCTTTACATCCCCAAATCCGCCACAACCTCTTCCGCTAAACTCCCTCTTCTTGTTTACTTCCACGGTGGCGGCTTCTGTGTTGACACTGCCTCTTCTCCTACTTACCACAACTACCTCAACTCCTTAGTAGCGGAAGCTAATGTTGTTGCTGTGTCTGTTGACTTTAGGAGAGTCCCAGAGTACCCTCTGCCTGTTGCCTACAATGATTCCTGGGATGCTCTCAAATGGGTTGCTTCTCATTCTGATGGAAGTGGCTCTGAGGAGTGGCTAAATAACCATGCAGATTTTCAGAAACTGTTCTTCTCTGGTGATAGTTCTGGTGGTAATATAGCGCATAACATGGCTGTGAAAGTCGGCTCTGAAGGCTTGGTTGGTGTTAAGCTCATAGGGATTGTGCTGGTGCATCCCTTTCTTTGGGGGAAAGAACCAATTGGGGGAGAGTCAACTATGCCTGCAGTTCAAAGAGAGTATCTGGATTCTATGTGGCGTTTTGTGTACTCTTTGACTAGCGGATCTGATGATCCGCTTCTCAATCCGGGTAAGGATCCGAAATTGGGTGGATTGGGGTGTGATAAAGTGTTGGTTTGTGTTGCTGAGAATGATACATTGAAACATAGAAACTGGTATTACAGTGAGGTCCTTAGAAAGAGTGGATGGAAAGGGGCTGTGGAGGTCTTGGAAGCAAAGGGGAGGGCCATGTTTTCCATTTGTTCAATCCAACTTGTGACAGTGCTAAGGCCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

304

Amino Acids

33.38

Weight (kDa)

5.85

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 42 - 112 5.4e-09 Carboxylesterase family
BD-FAE PF20434 58 - 175 1.4e-10 BD-FAE
Abhydrolase_3 PF07859 73 - 286 3e-49 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AciI CCGC 6 cut(s) 186, 201, 237, 299, 678, 692
AclWI GGATC 4 cut(s) 683, 688, 707, 720
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 2 cut(s) 350, 665
AfiI CCNNNNNNNGG 4 cut(s) 113, 582, 602, 722
AgsI TTSAA 4 cut(s) 144, 629, 778, 882
AjnI CCWGG 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
AluBI AGCT 2 cut(s) 305, 550
AluI AGCT 2 cut(s) 305, 550
AlwI GGATC 4 cut(s) 683, 688, 707, 720
AlwNI CAGNNNCTG 1 cut(s) 463
AoxI GGCC 2 cut(s) 861, 905
Asp700I GAANNNNTTC 1 cut(s) 464
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 2 cut(s) 805, 861
AsuC2I CCSGG 1 cut(s) 705
AsuHPI GGTGA 1 cut(s) 488
AvaII GGWCC 1 cut(s) 805
BaeGI GKGCMC 1 cut(s) 80
BamHI GGATCC 1 cut(s) 712
BccI CCATC 4 cut(s) 47, 103, 407, 816
BciT130I CCWGG 1 cut(s) 379
BcnI CCSGG 1 cut(s) 705
BfaI CTAG 2 cut(s) 165, 675
BfmI CTRYAG 1 cut(s) 621
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 379, 705
Bme18I GGWCC 1 cut(s) 805
BmgT120I GGNCC 2 cut(s) 805, 861
BmiI GGNNCC 1 cut(s) 714
BmrFI CCNGG 2 cut(s) 379, 705
BmsI GCATC 2 cut(s) 373, 580
Bpu10I CCTNAGC 1 cut(s) 902
BpuMI CCSGG 1 cut(s) 705
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 229, 378
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 272, 302
Bsc4I CCNNNNNNNGG 4 cut(s) 113, 582, 602, 722
Bse1I ACTGG 2 cut(s) 118, 794
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 2 cut(s) 229, 378
BseGI GGATG 5 cut(s) 103, 174, 388, 571, 827
BseLI CCNNNNNNNGG 4 cut(s) 113, 582, 602, 722
BseMII CTCAG 2 cut(s) 417, 753
BseNI ACTGG 2 cut(s) 118, 794
BseRI GAGGAG 2 cut(s) 21, 443
BseSI GKGCMC 1 cut(s) 80
BshFI GGCC 2 cut(s) 863, 907
BsiSI CCGG 1 cut(s) 704
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 4 cut(s) 113, 582, 602, 722
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 2 cut(s) 863, 907
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 3 cut(s) 680, 688, 712
BspACI CCGC 6 cut(s) 186, 201, 237, 299, 678, 692
BspANI GGCC 2 cut(s) 863, 907
BspCNI CTCAG 2 cut(s) 418, 754
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 714
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 4 cut(s) 683, 688, 707, 720
BsrI ACTGG 2 cut(s) 118, 794
BssECI CCNNGG 2 cut(s) 229, 378
BssMI GATC 3 cut(s) 680, 688, 712
BssNI GRCGYC 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 5 cut(s) 88, 233, 465, 800, 898
Bst6I CTCTTC 3 cut(s) 202, 219, 265
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 5 cut(s) 292, 426, 762, 809, 902
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 5 cut(s) 103, 174, 388, 571, 827
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 3 cut(s) 683, 691, 715
BstMBI GATC 3 cut(s) 680, 688, 712
BstMWI GCNNNNNNNGC 1 cut(s) 531
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 377, 703
BstSFI CTRYAG 1 cut(s) 621
BstSLI GKGCMC 1 cut(s) 80
BstX2I RGATCY 2 cut(s) 680, 712
BstYI RGATCY 2 cut(s) 680, 712
BsuRI GGCC 2 cut(s) 863, 907
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 5 cut(s) 103, 174, 388, 571, 827
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 3 cut(s) 252, 805, 903
Cac8I GCNNGC 1 cut(s) 621
CaiI CAGNNNCTG 1 cut(s) 463
CciI TCATGA 1 cut(s) 135
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 2 cut(s) 805, 861
Csp6I GTAC 2 cut(s) 349, 664
CviAII CATG 7 cut(s) 20, 73, 136, 446, 508, 865, 909
CviQI GTAC 2 cut(s) 349, 664
DdeI CTNAG 5 cut(s) 292, 426, 762, 809, 902
DpnI GATC 3 cut(s) 682, 690, 714
DpnII GATC 3 cut(s) 680, 688, 712
DraI TTTAAA 1 cut(s) 40
Eam1104I CTCTTC 3 cut(s) 202, 219, 265
EarI CTCTTC 3 cut(s) 202, 219, 265
EciI GGCGGA 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 805
Eco57I CTGAAG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 377
FaeI CATG 7 cut(s) 23, 76, 139, 449, 511, 868, 912
FaqI GGGAC 1 cut(s) 326
FatI CATG 7 cut(s) 19, 72, 135, 445, 507, 864, 908
Fnu4HI GCNGC 1 cut(s) 238
FokI GGATG 5 cut(s) 90, 161, 395, 558, 834
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 2 cut(s) 165, 675
GlaI GCGC 1 cut(s) 501
GluI GCNGC 1 cut(s) 238
HaeIII GGCC 2 cut(s) 863, 907
HapII CCGG 1 cut(s) 704
HhaI GCGC 1 cut(s) 502
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 7 cut(s) 23, 76, 139, 449, 511, 868, 912
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 3 cut(s) 250, 328, 612
HindII GTYRAC 3 cut(s) 250, 328, 612
HinfI GANTC 6 cut(s) 69, 122, 339, 374, 608, 644
HpaII CCGG 1 cut(s) 704
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 5 cut(s) 223, 250, 328, 612, 664
Hpy188I TCNGA 6 cut(s) 412, 427, 459, 529, 685, 717
Hpy188III TCNNGA 2 cut(s) 136, 641
Hpy8I GTNNAC 5 cut(s) 223, 250, 328, 612, 664
HpyAV CCTTC 1 cut(s) 524
HpyCH4III ACNGT 5 cut(s) 88, 233, 465, 800, 898
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 3 cut(s) 449, 571, 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 531
HpyF3I CTNAG 5 cut(s) 292, 426, 762, 809, 902
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 7 cut(s) 23, 76, 139, 449, 511, 868, 912
HspAI GCGC 1 cut(s) 500
Kzo9I GATC 3 cut(s) 680, 688, 712
LweI GCATC 2 cut(s) 373, 580
MaeI CTAG 2 cut(s) 165, 675
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 892
MalI GATC 3 cut(s) 682, 690, 714
MboI GATC 3 cut(s) 680, 688, 712
MboII GAAGA 4 cut(s) 189, 206, 252, 460
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 2 cut(s) 680, 712
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 3 cut(s) 60, 597, 719
MlyI GAGTC 3 cut(s) 63, 348, 617
MmeI TCCRAC 1 cut(s) 910
MroXI GAANNNNTTC 1 cut(s) 464
MseI TTAA 2 cut(s) 39, 546
MslI CAYNNNNRTG 2 cut(s) 411, 512
MspI CCGG 1 cut(s) 704
MspR9I CCNGG 2 cut(s) 379, 705
MunI CAATTG 1 cut(s) 597
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 531
NciI CCSGG 1 cut(s) 705
NdeII GATC 3 cut(s) 680, 688, 712
NlaIII CATG 7 cut(s) 23, 76, 139, 449, 511, 868, 912
NlaIV GGNNCC 1 cut(s) 714
NmuCI GTSAC 1 cut(s) 892
PagI TCATGA 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 3 cut(s) 122, 374, 644
PkrI GCNGC 1 cut(s) 239
PleI GAGTC 3 cut(s) 63, 347, 616
PpsI GAGTC 3 cut(s) 63, 347, 616
PpuMI RGGWCCY 1 cut(s) 805
Psp5II RGGWCCY 1 cut(s) 805
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 1 cut(s) 714
PspPI GGNCC 2 cut(s) 805, 861
PspPPI RGGWCCY 1 cut(s) 805
PstI CTGCAG 1 cut(s) 625
PstNI CAGNNNCTG 1 cut(s) 463
PsuI RGATCY 2 cut(s) 680, 712
RsaI GTAC 2 cut(s) 350, 665
RsaNI GTAC 2 cut(s) 349, 664
RseI CAYNNNNRTG 2 cut(s) 411, 512
SaqAI TTAA 2 cut(s) 39, 546
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 3 cut(s) 680, 688, 712
Sau96I GGNCC 2 cut(s) 805, 861
SchI GAGTC 3 cut(s) 63, 348, 617
ScrFI CCNGG 2 cut(s) 379, 705
SduI GDGCHC 1 cut(s) 80
SetI ASST 7 cut(s) 135, 197, 285, 307, 552, 807, 843
SfaNI GCATC 2 cut(s) 373, 580
SfcI CTRYAG 1 cut(s) 621
SinI GGWCC 1 cut(s) 805
SmiMI CAYNNNNRTG 2 cut(s) 411, 512
Sse9I AATT 3 cut(s) 60, 597, 719
SsiI CCGC 6 cut(s) 186, 201, 237, 299, 678, 692
SspMI CTAG 2 cut(s) 165, 675
StyD4I CCNGG 2 cut(s) 377, 703
TaaI ACNGT 5 cut(s) 88, 233, 465, 800, 898
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 58, 120
TasI AATT 3 cut(s) 60, 597, 719
TatI WGTACW 1 cut(s) 663
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 3 cut(s) 122, 374, 644
Tru1I TTAA 2 cut(s) 39, 546
Tru9I TTAA 2 cut(s) 39, 546
TscAI CASTG 3 cut(s) 259, 805, 903
TseFI GTSAC 1 cut(s) 892
Tsp45I GTSAC 1 cut(s) 892
TspDTI ATGAA 1 cut(s) 92
TspRI CASTG 3 cut(s) 259, 805, 903
VpaK11BI GGWCC 1 cut(s) 805
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 2 cut(s) 165, 675
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.