MD10G1091600.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000397
Physical Location & Seq
Reverse (-)
13856971 .. 13857609
639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1091600.v1.1.491

Sequence Viewer

Length: 639 bp
ATGAGCGACGAAGAATTAGCCCACGATTTTTCTCCGATTATCAAAGTATACAAAGATGGTCGAGTCGAGAGATCTTTAGGCACATCCACAGTTCCTCCATCAACACATCCCCAAACCGGTGTCCAATCCAAAGACGTCGTGATCTCACAAGAACCACCAGTATCCGCCAGGCTCTACCTTCCAACCAAAGCCACCGCTACAACCAAACTCTCTCTTCTTGTTTACTTCCACGGTGGCGGCTTCTGCATTGAAGGCTCCTCCTCCCCCACGTATCACAACTACCTCAACTCCTTAGTCTCCGGTGCCAATGTTGTTGCAGTCTCTGTTAATTACCGGCTTGCCCCCGAGCACCCTCTGCCGGCTGCTTACGATGATTCGTGGGCTGCTGTCAAATGGGTTGCTTCCCATTTCGATGGAAACGGCTCAGAAGATTGGTTAAACCTCCATGCGGATTTTCATCGTGTTTTCTTCGCCGGCGACAGTGCTGGGGCTAATATAGCTCACCAGATGGGATTGAGAGTAGGTTCTGAGGGCATGGTTGGTGGTGCTAAGCTGATTGGGGTTGTTTTGATCTATCCATATTTCTGGGGGACAGAGCCAGTTGGGGCGGAGGAGCTGTCCACGCCTGCACTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

213

Amino Acids

22.76

Weight (kDa)

5.65

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 58 - 175 9.7e-13 BD-FAE
COesterase PF00135 59 - 180 5.6e-08 Carboxylesterase family
Abhydrolase_3 PF07859 73 - 197 1.1e-39 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccB1I GGYRCC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 48
AciI CCGC 5 cut(s) 165, 195, 237, 449, 608
AcyI GRCGYC 1 cut(s) 135
AfiI CCNNNNNNNGG 3 cut(s) 116, 358, 448
AgeI ACCGGT 1 cut(s) 116
AgsI TTSAA 1 cut(s) 251
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 3 cut(s) 500, 553, 616
AluI AGCT 3 cut(s) 500, 553, 616
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 2 cut(s) 301, 325
AlwNI CAGNNNCTG 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 344
ApeKI GCWGC 2 cut(s) 362, 383
AsiGI ACCGGT 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 494
AvaI CYCGRG 1 cut(s) 344
BanI GGYRCC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 349, 370
BccI CCATC 4 cut(s) 50, 106, 407, 502
BceAI ACGGC 1 cut(s) 436
BciT130I CCWGG 1 cut(s) 169
BciVI GTATCC 1 cut(s) 172
BcoDI GTCTC 2 cut(s) 301, 325
BfuI GTATCC 1 cut(s) 172
BglII AGATCT 1 cut(s) 71
BisI GCNGC 3 cut(s) 238, 363, 384
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 3 cut(s) 239, 364, 385
Bme1390I CCNGG 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 344
BmiI GGNNCC 2 cut(s) 256, 304
BmrFI CCNGG 1 cut(s) 169
Bpu1102I GCTNAGC 1 cut(s) 549
BsaAI YACGTR 1 cut(s) 270
BsaBI GATNNNNATC 1 cut(s) 456
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 229
BsaWI WCCGGW 2 cut(s) 116, 299
BsaXI ACNNNNNCTCC 6 cut(s) 79, 109, 272, 302, 602, 632
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 358, 448
Bse118I RCCGGY 4 cut(s) 116, 333, 358, 473
Bse1I ACTGG 2 cut(s) 158, 599
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 2 cut(s) 83, 106
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 3 cut(s) 116, 358, 448
BseMII CTCAG 2 cut(s) 438, 519
BseNI ACTGG 2 cut(s) 158, 599
BseRI GAGGAG 3 cut(s) 247, 250, 626
BseXI GCAGC 2 cut(s) 349, 370
BseYI CCCAGC 1 cut(s) 485
BsgI GTGCAG 1 cut(s) 612
BshNI GGYRCC 1 cut(s) 302
BshTI ACCGGT 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 5 cut(s) 117, 300, 334, 359, 474
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 3 cut(s) 116, 358, 448
BsmAI GTCTC 2 cut(s) 301, 325
BsmFI GGGAC 1 cut(s) 604
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 3 cut(s) 71, 141, 570
Bsp1720I GCTNAGC 1 cut(s) 549
BspACI CCGC 5 cut(s) 165, 195, 237, 449, 608
BspCNI CTCAG 2 cut(s) 437, 520
BspLI GGNNCC 2 cut(s) 256, 304
BspT107I GGYRCC 1 cut(s) 302
BsrFI RCCGGY 4 cut(s) 116, 333, 358, 473
BsrI ACTGG 2 cut(s) 158, 599
BssAI RCCGGY 4 cut(s) 116, 333, 358, 473
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 3 cut(s) 71, 141, 570
BssNAI GTATAC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 135
Bst1107I GTATAC 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 3 cut(s) 91, 233, 482
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 135
BstAPI GCANNNNNTGC 1 cut(s) 355
BstBAI YACGTR 1 cut(s) 270
BstC8I GCNNGC 4 cut(s) 339, 360, 475, 627
BstDEI CTNAG 4 cut(s) 292, 424, 528, 549
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 2 cut(s) 83, 106
BstKTI GATC 3 cut(s) 74, 144, 573
BstMAI GTCTC 2 cut(s) 301, 325
BstMBI GATC 3 cut(s) 71, 141, 570
BstMWI GCNNNNNNNGC 5 cut(s) 243, 252, 355, 497, 622
BstNI CCWGG 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 167
BstV1I GCAGC 2 cut(s) 349, 370
BstX2I RGATCY 1 cut(s) 71
BstXI CCANNNNNNTGG 2 cut(s) 413, 585
BstYI RGATCY 1 cut(s) 71
BstZ17I GTATAC 1 cut(s) 49
BsuI GTATCC 1 cut(s) 172
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 2 cut(s) 83, 106
BtsIMutI CAGTG 1 cut(s) 487
Cac8I GCNNGC 4 cut(s) 339, 360, 475, 627
CaiI CAGNNNCTG 1 cut(s) 323
Cfr10I RCCGGY 4 cut(s) 116, 333, 358, 473
CspAI ACCGGT 1 cut(s) 116
CviAII CATG 2 cut(s) 446, 535
DdeI CTNAG 4 cut(s) 292, 424, 528, 549
DpnI GATC 3 cut(s) 73, 143, 572
DpnII GATC 3 cut(s) 71, 141, 570
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
EciI GGCGGA 2 cut(s) 154, 623
Eco88I CYCGRG 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 167
FaeI CATG 2 cut(s) 449, 538
FaiI YATR 5 cut(s) 49, 447, 497, 536, 580
FaqI GGGAC 1 cut(s) 604
FatI CATG 2 cut(s) 445, 534
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 238, 363, 384
FokI GGATG 2 cut(s) 70, 93
Fsp4HI GCNGC 3 cut(s) 238, 363, 384
GluI GCNGC 3 cut(s) 238, 363, 384
GsaI CCCAGC 1 cut(s) 489
HapII CCGG 5 cut(s) 117, 300, 334, 359, 474
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 2 cut(s) 449, 538
HinfI GANTC 2 cut(s) 63, 374
HpaII CCGG 5 cut(s) 117, 300, 334, 359, 474
HphI GGTGA 1 cut(s) 494
Hpy166II GTNNAC 3 cut(s) 49, 223, 621
Hpy188I TCNGA 3 cut(s) 36, 427, 529
Hpy188III TCNNGA 2 cut(s) 67, 139
Hpy8I GTNNAC 3 cut(s) 49, 223, 621
Hpy99I CGWCG 2 cut(s) 11, 140
HpyAV CCTTC 2 cut(s) 188, 245
HpyCH4III ACNGT 3 cut(s) 91, 233, 482
HpyCH4IV ACGT 2 cut(s) 135, 269
HpyCH4V TGCA 3 cut(s) 246, 317, 629
HpyF10VI GCNNNNNNNGC 5 cut(s) 243, 252, 355, 497, 622
HpyF3I CTNAG 4 cut(s) 292, 424, 528, 549
HpySE526I ACGT 2 cut(s) 135, 269
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 2 cut(s) 449, 538
KroI GCCGGC 2 cut(s) 358, 473
KroNI GCCGGC 2 cut(s) 360, 475
Kzo9I GATC 3 cut(s) 71, 141, 570
LmnI GCTCC 2 cut(s) 260, 613
Lsp1109I GCAGC 2 cut(s) 349, 370
MaeII ACGT 2 cut(s) 135, 269
MalI GATC 3 cut(s) 73, 143, 572
MboI GATC 3 cut(s) 71, 141, 570
MboII GAAGA 4 cut(s) 23, 206, 440, 460
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 2 cut(s) 14, 328
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 8 cut(s) 105, 268, 271, 293, 363, 452, 523, 604
MreI CGCCGGCG 1 cut(s) 473
MroNI GCCGGC 2 cut(s) 358, 473
MseI TTAA 3 cut(s) 327, 437, 633
MslI CAYNNNNRTG 2 cut(s) 411, 634
MspI CCGG 5 cut(s) 117, 300, 334, 359, 474
MspR9I CCNGG 1 cut(s) 169
MvaI CCWGG 1 cut(s) 169
MwoI GCNNNNNNNGC 5 cut(s) 243, 252, 355, 497, 622
NaeI GCCGGC 2 cut(s) 360, 475
NdeII GATC 3 cut(s) 71, 141, 570
NgoMIV GCCGGC 2 cut(s) 358, 473
NlaIII CATG 2 cut(s) 449, 538
NlaIV GGNNCC 2 cut(s) 256, 304
PdiI GCCGGC 2 cut(s) 360, 475
PfeI GAWTC 1 cut(s) 374
PinAI ACCGGT 1 cut(s) 116
PkrI GCNGC 3 cut(s) 239, 364, 385
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 485
PspGI CCWGG 1 cut(s) 167
PspN4I GGNNCC 2 cut(s) 256, 304
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 71
RseI CAYNNNNRTG 2 cut(s) 411, 634
SaqAI TTAA 3 cut(s) 327, 437, 633
SatI GCNGC 3 cut(s) 238, 363, 384
Sau3AI GATC 3 cut(s) 71, 141, 570
SchI GAGTC 1 cut(s) 72
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 1 cut(s) 351
SetI ASST 9 cut(s) 138, 180, 272, 285, 444, 502, 526, 555, 618
SgrAI CRCCGGYG 1 cut(s) 473
SmiMI CAYNNNNRTG 2 cut(s) 411, 634
Sse9I AATT 2 cut(s) 14, 328
SsiI CCGC 5 cut(s) 165, 195, 237, 449, 608
StyD4I CCNGG 1 cut(s) 167
TaaI ACNGT 3 cut(s) 91, 233, 482
TaiI ACGT 2 cut(s) 138, 272
TaqI TCGA 3 cut(s) 61, 66, 411
TasI AATT 2 cut(s) 14, 328
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 3 cut(s) 327, 437, 633
Tru9I TTAA 3 cut(s) 327, 437, 633
TscAI CASTG 1 cut(s) 487
TseI GCWGC 2 cut(s) 362, 383
TspDTI ATGAA 1 cut(s) 446
TspRI CASTG 1 cut(s) 487
XmiI GTMKAC 1 cut(s) 48
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.