Carboxylesterase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 581 |
| AciI | CCGC | 3 cut(s) | 579, 875, 929 |
| AclWI | GGATC | 7 cut(s) | 290, 591, 604, 875, 899, 912, 1062 |
| AcoI | YGGCCR | 1 cut(s) | 247 |
| AcsI | RAATTY | 1 cut(s) | 634 |
| AcuI | CTGAAG | 2 cut(s) | 222, 855 |
| AfaI | GTAC | 1 cut(s) | 207 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 181, 305, 607, 777, 870, 901, 914 |
| AgeI | ACCGGT | 1 cut(s) | 305 |
| AgsI | TTSAA | 3 cut(s) | 566, 1003, 1072 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 647, 679, 950, 982 |
| AluBI | AGCT | 6 cut(s) | 403, 474, 745, 815, 839, 997 |
| AluI | AGCT | 6 cut(s) | 403, 474, 745, 815, 839, 997 |
| AlwI | GGATC | 7 cut(s) | 290, 591, 604, 875, 899, 912, 1062 |
| Ama87I | CYCGRG | 1 cut(s) | 895 |
| AoxI | GGCC | 5 cut(s) | 19, 27, 247, 534, 576 |
| ApeKI | GCWGC | 1 cut(s) | 926 |
| ApoI | RAATTY | 1 cut(s) | 634 |
| AsiGI | ACCGGT | 1 cut(s) | 305 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 659 |
| AspLEI | GCGC | 1 cut(s) | 583 |
| AspS9I | GGNCC | 4 cut(s) | 28, 535, 576, 617 |
| AsuC2I | CCSGG | 2 cut(s) | 896, 897 |
| AsuHPI | GGTGA | 2 cut(s) | 441, 683 |
| AvaI | CYCGRG | 1 cut(s) | 895 |
| AvaII | GGWCC | 1 cut(s) | 617 |
| BamHI | GGATCC | 2 cut(s) | 596, 904 |
| BanII | GRGCYC | 1 cut(s) | 351 |
| BauI | CACGAG | 1 cut(s) | 865 |
| BbvI | GCAGC | 1 cut(s) | 938 |
| BccI | CCATC | 2 cut(s) | 823, 956 |
| BceAI | ACGGC | 1 cut(s) | 262 |
| BciVI | GTATCC | 1 cut(s) | 178 |
| BcnI | CCSGG | 2 cut(s) | 896, 897 |
| BfmI | CTRYAG | 1 cut(s) | 810 |
| BfuI | GTATCC | 1 cut(s) | 178 |
| BisI | GCNGC | 2 cut(s) | 579, 927 |
| BlsI | GCNGC | 2 cut(s) | 580, 928 |
| Bme1390I | CCNGG | 2 cut(s) | 896, 897 |
| Bme18I | GGWCC | 1 cut(s) | 617 |
| BmeT110I | CYCGRG | 1 cut(s) | 895 |
| BmgT120I | GGNCC | 4 cut(s) | 28, 535, 576, 617 |
| BmiI | GGNNCC | 3 cut(s) | 537, 598, 906 |
| BmrFI | CCNGG | 2 cut(s) | 896, 897 |
| BmsI | GCATC | 1 cut(s) | 775 |
| BpuMI | CCSGG | 2 cut(s) | 896, 897 |
| BsaBI | GATNNNNATC | 1 cut(s) | 189 |
| BsaJI | CCNNGG | 4 cut(s) | 180, 424, 486, 895 |
| BsaWI | WCCGGW | 1 cut(s) | 305 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1009, 1039 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 181, 305, 607, 777, 870, 901, 914 |
| Bse118I | RCCGGY | 1 cut(s) | 305 |
| Bse1I | ACTGG | 1 cut(s) | 791 |
| Bse8I | GATNNNNATC | 1 cut(s) | 189 |
| BseDI | CCNNGG | 4 cut(s) | 180, 424, 486, 895 |
| BseGI | GGATG | 4 cut(s) | 366, 766, 883, 967 |
| BseJI | GATNNNNATC | 1 cut(s) | 189 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 181, 305, 607, 777, 870, 901, 914 |
| BseMII | CTCAG | 3 cut(s) | 77, 348, 945 |
| BseNI | ACTGG | 1 cut(s) | 791 |
| BseRI | GAGGAG | 3 cut(s) | 157, 160, 1064 |
| BseX3I | CGGCCG | 1 cut(s) | 247 |
| BseXI | GCAGC | 1 cut(s) | 938 |
| BseYI | CCCAGC | 1 cut(s) | 680 |
| Bsh1236I | CGCG | 1 cut(s) | 581 |
| Bsh1285I | CGRYCG | 1 cut(s) | 250 |
| BshFI | GGCC | 5 cut(s) | 21, 29, 249, 536, 578 |
| BshTI | ACCGGT | 1 cut(s) | 305 |
| BsiEI | CGRYCG | 1 cut(s) | 250 |
| BsiHKCI | CYCGRG | 1 cut(s) | 895 |
| BsiSI | CCGG | 2 cut(s) | 306, 896 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 181, 305, 607, 777, 870, 901, 914 |
| BsnI | GGCC | 5 cut(s) | 21, 29, 249, 536, 578 |
| BsoBI | CYCGRG | 1 cut(s) | 895 |
| Bsp1286I | GDGCHC | 1 cut(s) | 351 |
| Bsp143I | GATC | 6 cut(s) | 295, 330, 596, 880, 904, 1054 |
| Bsp19I | CCATGG | 2 cut(s) | 180, 424 |
| BspACI | CCGC | 3 cut(s) | 579, 875, 929 |
| BspANI | GGCC | 5 cut(s) | 21, 29, 249, 536, 578 |
| BspCNI | CTCAG | 3 cut(s) | 76, 347, 946 |
| BspFNI | CGCG | 1 cut(s) | 581 |
| BspLI | GGNNCC | 3 cut(s) | 537, 598, 906 |
| BspPI | GGATC | 7 cut(s) | 290, 591, 604, 875, 899, 912, 1062 |
| BsrFI | RCCGGY | 1 cut(s) | 305 |
| BsrI | ACTGG | 1 cut(s) | 791 |
| BssAI | RCCGGY | 1 cut(s) | 305 |
| BssECI | CCNNGG | 4 cut(s) | 180, 424, 486, 895 |
| BssMI | GATC | 6 cut(s) | 295, 330, 596, 880, 904, 1054 |
| BssSI | CACGAG | 1 cut(s) | 865 |
| BssT1I | CCWWGG | 3 cut(s) | 180, 424, 486 |
| Bst2BI | CACGAG | 1 cut(s) | 865 |
| Bst4CI | ACNGT | 4 cut(s) | 76, 355, 811, 992 |
| Bst6I | CTCTTC | 1 cut(s) | 106 |
| BstDEI | CTNAG | 4 cut(s) | 63, 334, 954, 1044 |
| BstDSI | CCRYGG | 2 cut(s) | 180, 424 |
| BstEII | GGTNACC | 1 cut(s) | 447 |
| BstF5I | GGATG | 4 cut(s) | 366, 766, 883, 967 |
| BstFNI | CGCG | 1 cut(s) | 581 |
| BstHHI | GCGC | 1 cut(s) | 583 |
| BstKTI | GATC | 6 cut(s) | 298, 333, 599, 883, 907, 1057 |
| BstMBI | GATC | 6 cut(s) | 295, 330, 596, 880, 904, 1054 |
| BstMCI | CGRYCG | 1 cut(s) | 250 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 692 |
| BstPI | GGTNACC | 1 cut(s) | 447 |
| BstSCI | CCNGG | 2 cut(s) | 894, 895 |
| BstSFI | CTRYAG | 1 cut(s) | 810 |
| BstUI | CGCG | 1 cut(s) | 581 |
| BstV1I | GCAGC | 1 cut(s) | 938 |
| BstX2I | RGATCY | 2 cut(s) | 596, 904 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 431, 627 |
| BstYI | RGATCY | 2 cut(s) | 596, 904 |
| BstZI | CGGCCG | 1 cut(s) | 247 |
| BsuI | GTATCC | 1 cut(s) | 178 |
| BsuRI | GGCC | 5 cut(s) | 21, 29, 249, 536, 578 |
| BtgI | CCRYGG | 2 cut(s) | 180, 424 |
| BtgZI | GCGATG | 1 cut(s) | 842 |
| BtsCI | GGATG | 4 cut(s) | 366, 766, 883, 967 |
| BtsIMutI | CAGTG | 4 cut(s) | 72, 198, 351, 997 |
| CfoI | GCGC | 1 cut(s) | 583 |
| Cfr10I | RCCGGY | 1 cut(s) | 305 |
| Cfr13I | GGNCC | 4 cut(s) | 28, 535, 576, 617 |
| Cfr9I | CCCGGG | 1 cut(s) | 895 |
| CseI | GACGC | 1 cut(s) | 332 |
| Csp6I | GTAC | 1 cut(s) | 206 |
| CspAI | ACCGGT | 1 cut(s) | 305 |
| CviAII | CATG | 6 cut(s) | 37, 181, 425, 573, 703, 1058 |
| CviQI | GTAC | 1 cut(s) | 206 |
| DdeI | CTNAG | 4 cut(s) | 63, 334, 954, 1044 |
| DpnI | GATC | 6 cut(s) | 297, 332, 598, 882, 906, 1056 |
| DpnII | GATC | 6 cut(s) | 295, 330, 596, 880, 904, 1054 |
| EaeI | YGGCCR | 1 cut(s) | 247 |
| EagI | CGGCCG | 1 cut(s) | 247 |
| Eam1104I | CTCTTC | 1 cut(s) | 106 |
| EarI | CTCTTC | 1 cut(s) | 106 |
| EciI | GGCGGA | 1 cut(s) | 890 |
| EclXI | CGGCCG | 1 cut(s) | 247 |
| Eco130I | CCWWGG | 3 cut(s) | 180, 424, 486 |
| Eco24I | GRGCYC | 1 cut(s) | 351 |
| Eco47I | GGWCC | 1 cut(s) | 617 |
| Eco52I | CGGCCG | 1 cut(s) | 247 |
| Eco57I | CTGAAG | 2 cut(s) | 222, 855 |
| Eco88I | CYCGRG | 1 cut(s) | 895 |
| Eco91I | GGTNACC | 1 cut(s) | 447 |
| EcoO109I | RGGNCCY | 1 cut(s) | 535 |
| EcoO65I | GGTNACC | 1 cut(s) | 447 |
| EcoRI | GAATTC | 1 cut(s) | 634 |
| EcoT14I | CCWWGG | 3 cut(s) | 180, 424, 486 |
| EcoT38I | GRGCYC | 1 cut(s) | 351 |
| ErhI | CCWWGG | 3 cut(s) | 180, 424, 486 |
| FaeI | CATG | 6 cut(s) | 40, 184, 428, 576, 706, 1061 |
| FatI | CATG | 6 cut(s) | 36, 180, 424, 572, 702, 1057 |
| Fnu4HI | GCNGC | 2 cut(s) | 579, 927 |
| FokI | GGATG | 4 cut(s) | 353, 753, 890, 974 |
| FriOI | GRGCYC | 1 cut(s) | 351 |
| Fsp4HI | GCNGC | 2 cut(s) | 579, 927 |
| GlaI | GCGC | 1 cut(s) | 582 |
| GluI | GCNGC | 2 cut(s) | 579, 927 |
| GsaI | CCCAGC | 1 cut(s) | 684 |
| HaeIII | GGCC | 5 cut(s) | 21, 29, 249, 536, 578 |
| HapII | CCGG | 2 cut(s) | 306, 896 |
| HgaI | GACGC | 1 cut(s) | 332 |
| HhaI | GCGC | 1 cut(s) | 583 |
| Hin1II | CATG | 6 cut(s) | 40, 184, 428, 576, 706, 1061 |
| Hin6I | GCGC | 1 cut(s) | 581 |
| HinP1I | GCGC | 1 cut(s) | 581 |
| HindIII | AAGCTT | 1 cut(s) | 401 |
| HinfI | GANTC | 5 cut(s) | 252, 360, 569, 803, 1040 |
| HpaII | CCGG | 2 cut(s) | 306, 896 |
| HphI | GGTGA | 2 cut(s) | 441, 683 |
| Hpy166II | GTNNAC | 3 cut(s) | 79, 208, 418 |
| Hpy188I | TCNGA | 7 cut(s) | 66, 337, 787, 861, 909, 1080, 1104 |
| Hpy8I | GTNNAC | 3 cut(s) | 79, 208, 418 |
| HpyAV | CCTTC | 3 cut(s) | 952, 1102, 1132 |
| HpyCH4III | ACNGT | 4 cut(s) | 76, 355, 811, 992 |
| HpyCH4V | TGCA | 2 cut(s) | 766, 973 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 692 |
| HpyF3I | CTNAG | 4 cut(s) | 63, 334, 954, 1044 |
| Hsp92II | CATG | 6 cut(s) | 40, 184, 428, 576, 706, 1061 |
| HspAI | GCGC | 1 cut(s) | 581 |
| Kzo9I | GATC | 6 cut(s) | 295, 330, 596, 880, 904, 1054 |
| LpnPI | CCDG | 8 cut(s) | 319, 330, 552, 633, 666, 804, 909, 926 |
| Lsp1109I | GCAGC | 1 cut(s) | 938 |
| LweI | GCATC | 1 cut(s) | 775 |
| MaeIII | GTNAC | 1 cut(s) | 447 |
| MalI | GATC | 6 cut(s) | 297, 332, 598, 882, 906, 1056 |
| MboI | GATC | 6 cut(s) | 295, 330, 596, 880, 904, 1054 |
| MboII | GAAGA | 4 cut(s) | 93, 96, 578, 1015 |
| MfeI | CAATTG | 1 cut(s) | 555 |
| MflI | RGATCY | 2 cut(s) | 596, 904 |
| MhlI | GDGCHC | 1 cut(s) | 351 |
| MluCI | AATT | 6 cut(s) | 144, 381, 555, 634, 714, 911 |
| MlyI | GAGTC | 2 cut(s) | 354, 1049 |
| MmeI | TCCRAC | 4 cut(s) | 308, 574, 884, 1103 |
| MroXI | GAANNNNTTC | 1 cut(s) | 659 |
| MseI | TTAA | 1 cut(s) | 143 |
| MslI | CAYNNNNRTG | 2 cut(s) | 429, 990 |
| MspA1I | CMGCKG | 1 cut(s) | 929 |
| MspI | CCGG | 2 cut(s) | 306, 896 |
| MspR9I | CCNGG | 2 cut(s) | 896, 897 |
| MunI | CAATTG | 1 cut(s) | 555 |
| MvnI | CGCG | 1 cut(s) | 581 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 692 |
| NciI | CCSGG | 2 cut(s) | 896, 897 |
| NcoI | CCATGG | 2 cut(s) | 180, 424 |
| NdeII | GATC | 6 cut(s) | 295, 330, 596, 880, 904, 1054 |
| NlaIII | CATG | 6 cut(s) | 40, 184, 428, 576, 706, 1061 |
| NlaIV | GGNNCC | 3 cut(s) | 537, 598, 906 |
| NmuCI | GTSAC | 1 cut(s) | 447 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 252 |
| PdmI | GAANNNNTTC | 1 cut(s) | 659 |
| PfeI | GAWTC | 3 cut(s) | 252, 569, 803 |
| PinAI | ACCGGT | 1 cut(s) | 305 |
| PkrI | GCNGC | 2 cut(s) | 580, 928 |
| PleI | GAGTC | 2 cut(s) | 354, 1048 |
| PpsI | GAGTC | 2 cut(s) | 354, 1048 |
| PspEI | GGTNACC | 1 cut(s) | 447 |
| PspFI | CCCAGC | 1 cut(s) | 680 |
| PspN4I | GGNNCC | 3 cut(s) | 537, 598, 906 |
| PspPI | GGNCC | 4 cut(s) | 28, 535, 576, 617 |
| PsuI | RGATCY | 2 cut(s) | 596, 904 |
| RsaI | GTAC | 1 cut(s) | 207 |
| RsaNI | GTAC | 1 cut(s) | 206 |
| RseI | CAYNNNNRTG | 2 cut(s) | 429, 990 |
| SaqAI | TTAA | 1 cut(s) | 143 |
| SatI | GCNGC | 2 cut(s) | 579, 927 |
| Sau3AI | GATC | 6 cut(s) | 295, 330, 596, 880, 904, 1054 |
| Sau96I | GGNCC | 4 cut(s) | 28, 535, 576, 617 |
| SchI | GAGTC | 2 cut(s) | 354, 1049 |
| ScrFI | CCNGG | 2 cut(s) | 896, 897 |
| SduI | GDGCHC | 1 cut(s) | 351 |
| SfaNI | GCATC | 1 cut(s) | 775 |
| SfcI | CTRYAG | 1 cut(s) | 810 |
| SinI | GGWCC | 1 cut(s) | 617 |
| SmaI | CCCGGG | 1 cut(s) | 897 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 429, 990 |
| Sse9I | AATT | 6 cut(s) | 144, 381, 555, 634, 714, 911 |
| SsiI | CCGC | 3 cut(s) | 579, 875, 929 |
| StyD4I | CCNGG | 2 cut(s) | 894, 895 |
| StyI | CCWWGG | 3 cut(s) | 180, 424, 486 |
| TaaI | ACNGT | 4 cut(s) | 76, 355, 811, 992 |
| TaqI | TCGA | 3 cut(s) | 255, 312, 806 |
| TasI | AATT | 6 cut(s) | 144, 381, 555, 634, 714, 911 |
| TatI | WGTACW | 1 cut(s) | 205 |
| TauI | GCSGC | 1 cut(s) | 581 |
| TfiI | GAWTC | 3 cut(s) | 252, 569, 803 |
| Tru1I | TTAA | 1 cut(s) | 143 |
| Tru9I | TTAA | 1 cut(s) | 143 |
| TscAI | CASTG | 4 cut(s) | 79, 205, 358, 997 |
| TseFI | GTSAC | 1 cut(s) | 447 |
| TseI | GCWGC | 1 cut(s) | 926 |
| Tsp45I | GTSAC | 1 cut(s) | 447 |
| TspDTI | ATGAA | 5 cut(s) | 96, 561, 1055, 1114, 1121 |
| TspGWI | ACGGA | 1 cut(s) | 873 |
| TspMI | CCCGGG | 1 cut(s) | 895 |
| TspRI | CASTG | 4 cut(s) | 79, 205, 358, 997 |
| VpaK11BI | GGWCC | 1 cut(s) | 617 |
| XapI | RAATTY | 1 cut(s) | 634 |
| XmaI | CCCGGG | 1 cut(s) | 895 |
| XmnI | GAANNNNTTC | 1 cut(s) | 659 |