Rroxscaffold_7G00205410

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000397
Physical Location & Seq
Forward (+)
54983996 .. 54984939
944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205410.1

Sequence Viewer

Length: 867 bp
ATGAGCGATGAACTAGCCCATGATTTCTTTCCCATCATCAGAGTCTACAAAGACGGTCGAGTTGACCGACTCTGGGGCACATCCACACTCCCTGCATCCATCGATCCCCAAACCGGTGTTCAATCCAAAGACGTCGTCATCTCAACCGCACCACCCGTCTCTGTAAGGCTTTACATTCCCAAATCCACCACAACCGAGTCAACCCGGAAGCTCCCTCTTCTGGTTTACTTTCACGGCGGTGGCTTCTGCAATGGAAGTGCTTTCTCTCCCACTTATCACAACTACCTCAACTCTTTAGTCTCTGAAGCCAATGTTGTAGCTGTTTCTGTTGACTATAGGTTGGTGCCGGAGCACCCTCTTCCGGCTGCCTATGAAGATTCATGGGCCGCTCTCAAATGGGTGGAGTCCCATTTCGCAGGAAACGGCCCCGAAGACTGGCTGAACCGGCTTGCAGATTTGAACCGGGTTTTCTTTTCAGGTGATAGCGCTGGGGCTAACATAGCTCACCAAATGGGTTTGAGAGTTGGGGGAGAGCTAGCTTTGGCTGAGAATCATACAAAGATGTTCGCTGCTTTGTGGAGGTTTTGTTACCCTTTGACTAGTGGATCCGATGACCCGGTTCTCAATCCGGGTAAGGATCCGAAGTTGGGAGAGTTGGGTTGTGAGAAAGTGTTGGTCTGCGTTGCGGAGAAGGATTCGTTGAAAGATAGGGGGTGGTATTACAGTGAGGTGTTGAAGAAGAGTGGATGGAATGGAGTAGTGGAGGTTATGGAAGCAAAGGAGGAGCAGCATGTGTTCCATTTGGTCAATCCTAGTTGTGACAATGCTGTGACCATGCTCAAGAAGTTAGTTTCTTTCATAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

288

Amino Acids

31.82

Weight (kDa)

5.72

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 57 - 170 2.6e-09 Carboxylesterase family
BD-FAE PF20434 66 - 176 6.1e-13 BD-FAE
Abhydrolase_3 PF07859 74 - 176 5.4e-34 alpha/beta hydrolase fold
Abhydrolase_3 PF07859 186 - 267 1.8e-10 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 343
AccBSI CCGCTC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 45
AciI CCGC 4 cut(s) 147, 237, 387, 686
AclWI GGATC 5 cut(s) 98, 600, 613, 632, 645
AcuI CTGAAG 1 cut(s) 324
AcyI GRCGYC 1 cut(s) 132
AfeI AGCGCT 1 cut(s) 487
AfiI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 435, 647
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 4 cut(s) 122, 460, 703, 736
AhlI ACTAGT 1 cut(s) 599
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 5 cut(s) 211, 320, 503, 535, 539
AluI AGCT 5 cut(s) 211, 320, 503, 535, 539
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 2 cut(s) 163, 304
AlwI GGATC 5 cut(s) 98, 600, 613, 632, 645
Aor51HI AGCGCT 1 cut(s) 487
AoxI GGCC 2 cut(s) 384, 424
ApeKI GCWGC 3 cut(s) 365, 569, 787
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 384, 425
AsuC2I CCSGG 4 cut(s) 205, 464, 617, 630
AsuHPI GGTGA 2 cut(s) 491, 497
AsuNHI GCTAGC 1 cut(s) 535
BaeGI GKGCMC 1 cut(s) 80
BamHI GGATCC 2 cut(s) 605, 637
BanI GGYRCC 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 3 cut(s) 352, 556, 799
BccI CCATC 3 cut(s) 41, 107, 741
BceAI ACGGC 2 cut(s) 250, 439
BcnI CCSGG 4 cut(s) 205, 464, 617, 630
BcoDI GTCTC 2 cut(s) 163, 304
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 4 cut(s) 14, 536, 600, 813
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 489
BisI GCNGC 4 cut(s) 366, 387, 570, 788
BlsI GCNGC 4 cut(s) 367, 388, 571, 789
Bme1390I CCNGG 4 cut(s) 205, 464, 617, 630
BmgT120I GGNCC 2 cut(s) 384, 425
BmiI GGNNCC 4 cut(s) 345, 427, 607, 639
BmrFI CCNGG 4 cut(s) 205, 464, 617, 630
BmsI GCATC 1 cut(s) 104
BmtI GCTAGC 1 cut(s) 539
BpiI GAAGAC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 824
BpuMI CCSGG 4 cut(s) 205, 464, 617, 630
Bsa29I ATCGAT 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 2 cut(s) 571, 601
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 435, 647
Bse118I RCCGGY 2 cut(s) 113, 444
Bse1I ACTGG 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 256
BseCI ATCGAT 1 cut(s) 102
BseGI GGATG 3 cut(s) 80, 95, 752
BseLI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 435, 647
BseMI GCAATG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 1 cut(s) 440
BseRI GAGGAG 1 cut(s) 797
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 3 cut(s) 352, 556, 799
BseYI CCCAGC 1 cut(s) 488
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 386, 426
BshNI GGYRCC 1 cut(s) 343
BshTI ACCGGT 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 102
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 8 cut(s) 114, 205, 347, 362, 445, 463, 617, 629
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 6 cut(s) 73, 113, 220, 361, 435, 647
BsmAI GTCTC 2 cut(s) 163, 304
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 2 cut(s) 386, 426
Bsp1286I GDGCHC 2 cut(s) 80, 354
Bsp143I GATC 3 cut(s) 103, 605, 637
BspACI CCGC 4 cut(s) 147, 237, 387, 686
BspANI GGCC 2 cut(s) 386, 426
BspCNI CTCAG 1 cut(s) 538
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 4 cut(s) 345, 427, 607, 639
BspOI GCTAGC 1 cut(s) 539
BspPI GGATC 5 cut(s) 98, 600, 613, 632, 645
BspT107I GGYRCC 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 256
BsrFI RCCGGY 2 cut(s) 113, 444
BsrI ACTGG 1 cut(s) 440
BssAI RCCGGY 2 cut(s) 113, 444
BssMI GATC 3 cut(s) 103, 605, 637
BssNI GRCGYC 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 56, 725
Bst6I CTCTTC 3 cut(s) 222, 363, 734
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 450, 537
BstDEI CTNAG 1 cut(s) 546
BstF5I GGATG 3 cut(s) 80, 95, 752
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 3 cut(s) 106, 608, 640
BstMAI GTCTC 2 cut(s) 163, 304
BstMBI GATC 3 cut(s) 103, 605, 637
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 2 cut(s) 445, 500
BstNSI RCATGY 1 cut(s) 794
BstSCI CCNGG 4 cut(s) 203, 462, 615, 628
BstSFI CTRYAG 1 cut(s) 334
BstSLI GKGCMC 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 352, 556, 799
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 605, 637
BstYI RGATCY 2 cut(s) 605, 637
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 2 cut(s) 386, 426
BsuTUI ATCGAT 1 cut(s) 102
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 3 cut(s) 80, 95, 752
BtsIMutI CAGTG 1 cut(s) 730
Cac8I GCNNGC 2 cut(s) 450, 537
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 2 cut(s) 113, 444
Cfr13I GGNCC 2 cut(s) 384, 425
ClaI ATCGAT 1 cut(s) 102
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 4 cut(s) 20, 381, 791, 835
DdeI CTNAG 1 cut(s) 546
DpnI GATC 3 cut(s) 105, 607, 639
DpnII GATC 3 cut(s) 103, 605, 637
Eam1104I CTCTTC 3 cut(s) 222, 363, 734
EarI CTCTTC 3 cut(s) 222, 363, 734
Eco47III AGCGCT 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 4 cut(s) 23, 384, 794, 838
FaqI GGGAC 1 cut(s) 391
FatI CATG 4 cut(s) 19, 380, 790, 834
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 4 cut(s) 366, 387, 570, 788
FokI GGATG 3 cut(s) 67, 82, 759
Fsp4HI GCNGC 4 cut(s) 366, 387, 570, 788
FspBI CTAG 4 cut(s) 14, 536, 600, 813
GlaI GCGC 1 cut(s) 487
GluI GCNGC 4 cut(s) 366, 387, 570, 788
GsaI CCCAGC 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 2 cut(s) 386, 426
HapII CCGG 8 cut(s) 114, 205, 347, 362, 445, 463, 617, 629
HhaI GCGC 1 cut(s) 488
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 4 cut(s) 23, 384, 794, 838
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 3 cut(s) 64, 201, 331
HindII GTYRAC 3 cut(s) 64, 201, 331
HinfI GANTC 7 cut(s) 42, 69, 197, 377, 404, 550, 695
HpaII CCGG 8 cut(s) 114, 205, 347, 362, 445, 463, 617, 629
HphI GGTGA 2 cut(s) 491, 497
Hpy166II GTNNAC 5 cut(s) 46, 64, 201, 226, 331
Hpy188I TCNGA 4 cut(s) 41, 304, 610, 642
Hpy188III TCNNGA 1 cut(s) 841
Hpy8I GTNNAC 5 cut(s) 46, 64, 201, 226, 331
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 685
HpyCH4III ACNGT 2 cut(s) 56, 725
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 3 cut(s) 95, 249, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 500
HpyF3I CTNAG 1 cut(s) 546
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 4 cut(s) 23, 384, 794, 838
HspAI GCGC 1 cut(s) 486
Kzo9I GATC 3 cut(s) 103, 605, 637
LmnI GCTCC 3 cut(s) 216, 349, 784
Lsp1109I GCAGC 3 cut(s) 352, 556, 799
LweI GCATC 1 cut(s) 104
MaeI CTAG 4 cut(s) 14, 536, 600, 813
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 587, 818, 829
MalI GATC 3 cut(s) 105, 607, 639
MbiI CCGCTC 1 cut(s) 389
MboI GATC 3 cut(s) 103, 605, 637
MboII GAAGA 6 cut(s) 209, 350, 386, 443, 748, 751
MflI RGATCY 2 cut(s) 605, 637
MhlI GDGCHC 2 cut(s) 80, 354
MluCI AATT 1 cut(s) 862
MlyI GAGTC 4 cut(s) 51, 63, 206, 413
MnlI CCTC 7 cut(s) 225, 296, 366, 573, 721, 757, 775
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 8 cut(s) 114, 205, 347, 362, 445, 463, 617, 629
MspR9I CCNGG 4 cut(s) 205, 464, 617, 630
MwoI GCNNNNNNNGC 2 cut(s) 445, 500
NciI CCSGG 4 cut(s) 205, 464, 617, 630
NdeII GATC 3 cut(s) 103, 605, 637
NheI GCTAGC 1 cut(s) 535
NlaIII CATG 4 cut(s) 23, 384, 794, 838
NlaIV GGNNCC 4 cut(s) 345, 427, 607, 639
NmuCI GTSAC 2 cut(s) 818, 829
NspI RCATGY 1 cut(s) 794
OliI CACNNNNGTG 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PfeI GAWTC 3 cut(s) 377, 550, 695
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 4 cut(s) 367, 388, 571, 789
PleI GAGTC 4 cut(s) 50, 63, 205, 412
PpsI GAGTC 4 cut(s) 50, 63, 205, 412
PspFI CCCAGC 1 cut(s) 488
PspN4I GGNNCC 4 cut(s) 345, 427, 607, 639
PspPI GGNCC 2 cut(s) 384, 425
PsuI RGATCY 2 cut(s) 605, 637
PsyI GACNNNGTC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 237
SatI GCNGC 4 cut(s) 366, 387, 570, 788
Sau3AI GATC 3 cut(s) 103, 605, 637
Sau96I GGNCC 2 cut(s) 384, 425
SchI GAGTC 4 cut(s) 51, 63, 206, 413
ScrFI CCNGG 4 cut(s) 205, 464, 617, 630
SduI GDGCHC 2 cut(s) 80, 354
SfaNI GCATC 1 cut(s) 104
SfcI CTRYAG 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 1 cut(s) 862
SsiI CCGC 4 cut(s) 147, 237, 387, 686
SspMI CTAG 4 cut(s) 14, 536, 600, 813
StyD4I CCNGG 4 cut(s) 203, 462, 615, 628
TaaI ACNGT 2 cut(s) 56, 725
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 58, 102
TaqII GACCGA 1 cut(s) 81
TasI AATT 1 cut(s) 862
TauI GCSGC 1 cut(s) 389
TfiI GAWTC 3 cut(s) 377, 550, 695
TscAI CASTG 1 cut(s) 730
TseFI GTSAC 2 cut(s) 818, 829
TseI GCWGC 3 cut(s) 365, 569, 787
Tsp45I GTSAC 2 cut(s) 818, 829
TspDTI ATGAA 4 cut(s) 24, 369, 387, 847
TspRI CASTG 1 cut(s) 730
Tth111I GACNNNGTC 1 cut(s) 134
XceI RCATGY 1 cut(s) 794
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 4 cut(s) 14, 536, 600, 813
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.