MD10G1091900.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000397
Physical Location & Seq
Reverse (-)
13902028 .. 13902966
939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1091900.v1.1.491

Sequence Viewer

Length: 939 bp
ATGACAAACGAAGTAGCCCATGATCTCTCTCCATTCATCAAAACATACAAAGATGGTCGAGTCGAGAGACTCTTAGGCACATCCACAGTCCCTCCATCAACGCCCCAAATCGGTGTCCAATCCAAGGACGTCGTGATCTCACAAGAACCACCCATATCCGCAAGGCTTTACCTCCCAAAATCCGCCACCAACTCAACCGACAAGCTCCCTCTTCTCATTTACTTCCATGGAGGGGGGTTCTGTGTGGAAAGCGCCTTTTCTCCCACATATCACAACTACCTCAACTCCTTAGTATCCGAGGCCAATGTTGTTGCTGTCTCTGTTGAATATAGGCTTGCCCCAGAGCACCCTCTTCCGGCTGCTTACGATGATTCATGGGATGCTGTTAAATGGGTTGCTTCTCATTTTGATGGGAACGGCTTTGAAGATTGGCTAAATCACTACGCGGATTGTCATCGCGTGTTCTTCGCTGGCGACAGCGCTGGGGCTAATATAGCACACAACCTGGCTTCGAAATTAGGGTCCGAGGGGTTGGATGGTGTTAAGCTCTTTGGGCTTGTATTGATACATCCATACTTTTGGGGGACAGAACCAGTCGGGGGAGAGTTAACTACCCCAGAAGCTACAAGAGAGTTTATGGCTGGTATGTGGCGTTTTGTGCACCCTTCGACTAGTGGATCCGATGATCTGCTTATAAACCCATGTAAGGATCCGAAACTGGCGGAGTTGGGTTGTGAGAAAGTGTTGATTTTTGCTGCTGAGAAAGATGGGTTGAAAGATAGGGCATGGCATTATGGTGAGACACTGAGAAAGTGTGGGTGGAGTGGGGCTGTGGAGGTGATAGAATCAAAGGGGGAGGAGCATGTCTTTCATTTGTTCAAACCCACTTGTGAGAATGCTGTGGCCATGAAGAAAAAGATTTCTTCTTTCTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

313

Amino Acids

34.34

Weight (kDa)

5.61

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 56 - 178 5.6e-12 BD-FAE
COesterase PF00135 56 - 114 2.8e-08 Carboxylesterase family
Abhydrolase_3 PF07859 72 - 291 2.4e-53 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
AatII GACGTC 1 cut(s) 132
AccII CGCG 2 cut(s) 446, 459
AciI CCGC 4 cut(s) 159, 183, 446, 722
AclWI GGATC 4 cut(s) 672, 685, 704, 717
AcoI YGGCCR 1 cut(s) 903
AcyI GRCGYC 1 cut(s) 129
AfeI AGCGCT 1 cut(s) 481
AfiI CCNNNNNNNGG 6 cut(s) 110, 124, 232, 355, 599, 706
AgsI TTSAA 5 cut(s) 326, 425, 775, 880, 934
AhlI ACTAGT 1 cut(s) 671
AjnI CCWGG 1 cut(s) 504
AluBI AGCT 3 cut(s) 205, 547, 623
AluI AGCT 3 cut(s) 205, 547, 623
Alw21I GWGCWC 2 cut(s) 348, 663
Alw26I GTCTC 3 cut(s) 61, 322, 794
Alw44I GTGCAC 1 cut(s) 659
AlwI GGATC 4 cut(s) 672, 685, 704, 717
Aor51HI AGCGCT 1 cut(s) 481
AoxI GGCC 2 cut(s) 300, 903
ApaLI GTGCAC 1 cut(s) 659
ApeKI GCWGC 2 cut(s) 359, 755
ArsI GACNNNNNNTTYG 2 cut(s) 506, 538
AspLEI GCGC 2 cut(s) 254, 482
AspS9I GGNCC 1 cut(s) 522
AsuHPI GGTGA 2 cut(s) 809, 850
AsuII TTCGAA 1 cut(s) 512
AvaII GGWCC 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 663
BalI TGGCCA 1 cut(s) 905
BamHI GGATCC 2 cut(s) 677, 709
Bbv12I GWGCWC 2 cut(s) 348, 663
BbvI GCAGC 2 cut(s) 346, 742
BccI CCATC 5 cut(s) 47, 103, 404, 530, 761
BceAI ACGGC 1 cut(s) 433
BciT130I CCWGG 1 cut(s) 506
BciVI GTATCC 1 cut(s) 304
BcoDI GTCTC 3 cut(s) 61, 322, 794
BcuI ACTAGT 1 cut(s) 671
BfaI CTAG 1 cut(s) 672
BfoI RGCGCY 2 cut(s) 255, 483
BfuI GTATCC 1 cut(s) 304
BisI GCNGC 2 cut(s) 360, 756
BlsI GCNGC 2 cut(s) 361, 757
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 1 cut(s) 522
BmgT120I GGNCC 1 cut(s) 522
BmiI GGNNCC 3 cut(s) 523, 679, 711
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 1 cut(s) 370
Bpu14I TTCGAA 1 cut(s) 512
BsaBI GATNNNNATC 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 4 cut(s) 123, 226, 297, 525
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 269, 299
Bsc4I CCNNNNNNNGG 6 cut(s) 110, 124, 232, 355, 599, 706
Bse1I ACTGG 2 cut(s) 593, 723
Bse8I GATNNNNATC 1 cut(s) 453
BseBI CCWGG 1 cut(s) 506
BseDI CCNNGG 4 cut(s) 123, 226, 297, 525
BseGI GGATG 4 cut(s) 80, 385, 541, 568
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 6 cut(s) 110, 124, 232, 355, 599, 706
BseMII CTCAG 2 cut(s) 750, 797
BseNI ACTGG 2 cut(s) 593, 723
BseRI GAGGAG 1 cut(s) 872
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 2 cut(s) 346, 742
BseYI CCCAGC 1 cut(s) 482
Bsh1236I CGCG 2 cut(s) 446, 459
BshFI GGCC 2 cut(s) 302, 905
BsiHKAI GWGCWC 2 cut(s) 348, 663
BsiSI CCGG 1 cut(s) 356
BslFI GGGAC 2 cut(s) 74, 598
BslI CCNNNNNNNGG 6 cut(s) 110, 124, 232, 355, 599, 706
BsmAI GTCTC 3 cut(s) 61, 322, 794
BsmFI GGGAC 2 cut(s) 74, 598
BsmI GAATGC 1 cut(s) 901
BsnI GGCC 2 cut(s) 302, 905
Bsp119I TTCGAA 1 cut(s) 512
Bsp1286I GDGCHC 2 cut(s) 348, 663
Bsp143I GATC 5 cut(s) 22, 135, 677, 685, 709
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 4 cut(s) 159, 183, 446, 722
BspANI GGCC 2 cut(s) 302, 905
BspCNI CTCAG 2 cut(s) 751, 798
BspFNI CGCG 2 cut(s) 446, 459
BspLI GGNNCC 3 cut(s) 523, 679, 711
BspPI GGATC 4 cut(s) 672, 685, 704, 717
BspT104I TTCGAA 1 cut(s) 512
BsrI ACTGG 2 cut(s) 593, 723
BssECI CCNNGG 4 cut(s) 123, 226, 297, 525
BssMI GATC 5 cut(s) 22, 135, 677, 685, 709
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 2 cut(s) 123, 226
Bst2UI CCWGG 1 cut(s) 506
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 2 cut(s) 216, 357
BstACI GRCGYC 1 cut(s) 129
BstBI TTCGAA 1 cut(s) 512
BstC8I GCNNGC 2 cut(s) 336, 472
BstDEI CTNAG 4 cut(s) 73, 289, 759, 806
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 4 cut(s) 80, 385, 541, 568
BstFNI CGCG 2 cut(s) 446, 459
BstH2I RGCGCY 2 cut(s) 255, 483
BstHHI GCGC 2 cut(s) 254, 482
BstKTI GATC 5 cut(s) 25, 138, 680, 688, 712
BstMAI GTCTC 3 cut(s) 61, 322, 794
BstMBI GATC 5 cut(s) 22, 135, 677, 685, 709
BstMWI GCNNNNNNNGC 3 cut(s) 494, 553, 658
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 1 cut(s) 866
BstSCI CCNGG 1 cut(s) 504
BstSLI GKGCMC 1 cut(s) 663
BstUI CGCG 2 cut(s) 446, 459
BstV1I GCAGC 2 cut(s) 346, 742
BstX2I RGATCY 2 cut(s) 677, 709
BstXI CCANNNNNNTGG 1 cut(s) 579
BstYI RGATCY 2 cut(s) 677, 709
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 2 cut(s) 302, 905
BtgI CCRYGG 1 cut(s) 226
BtgZI GCGATG 1 cut(s) 440
BtsCI GGATG 4 cut(s) 80, 385, 541, 568
BtsIMutI CAGTG 1 cut(s) 803
Cac8I GCNNGC 2 cut(s) 336, 472
CfoI GCGC 2 cut(s) 254, 482
Cfr13I GGNCC 1 cut(s) 522
CviAII CATG 7 cut(s) 20, 227, 375, 702, 786, 863, 907
DdeI CTNAG 4 cut(s) 73, 289, 759, 806
DpnI GATC 5 cut(s) 24, 137, 679, 687, 711
DpnII GATC 5 cut(s) 22, 135, 677, 685, 709
EaeI YGGCCR 1 cut(s) 903
Eam1104I CTCTTC 2 cut(s) 216, 357
EarI CTCTTC 2 cut(s) 216, 357
EciI GGCGGA 2 cut(s) 172, 737
Eco130I CCWWGG 2 cut(s) 123, 226
Eco47I GGWCC 1 cut(s) 522
Eco47III AGCGCT 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 2 cut(s) 123, 226
ErhI CCWWGG 2 cut(s) 123, 226
FaeI CATG 7 cut(s) 23, 230, 378, 705, 789, 866, 910
FaqI GGGAC 2 cut(s) 74, 598
FatI CATG 7 cut(s) 19, 226, 374, 701, 785, 862, 906
Fnu4HI GCNGC 2 cut(s) 360, 756
FokI GGATG 4 cut(s) 67, 392, 548, 555
Fsp4HI GCNGC 2 cut(s) 360, 756
FspBI CTAG 1 cut(s) 672
GlaI GCGC 2 cut(s) 253, 481
GluI GCNGC 2 cut(s) 360, 756
GsaI CCCAGC 1 cut(s) 486
HaeII RGCGCY 2 cut(s) 255, 483
HaeIII GGCC 2 cut(s) 302, 905
HapII CCGG 1 cut(s) 356
HhaI GCGC 2 cut(s) 254, 482
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 7 cut(s) 23, 230, 378, 705, 789, 866, 910
Hin6I GCGC 2 cut(s) 252, 480
HinP1I GCGC 2 cut(s) 252, 480
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 4 cut(s) 60, 69, 371, 845
HpaI GTTAAC 1 cut(s) 609
HpaII CCGG 1 cut(s) 356
HphI GGTGA 2 cut(s) 809, 850
Hpy166II GTNNAC 2 cut(s) 609, 661
Hpy188I TCNGA 4 cut(s) 298, 526, 682, 714
Hpy188III TCNNGA 3 cut(s) 64, 133, 931
Hpy8I GTNNAC 2 cut(s) 609, 661
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 675
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 661
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 553, 658
HpyF3I CTNAG 4 cut(s) 73, 289, 759, 806
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 7 cut(s) 23, 230, 378, 705, 789, 866, 910
HspAI GCGC 2 cut(s) 252, 480
KspAI GTTAAC 1 cut(s) 609
Kzo9I GATC 5 cut(s) 22, 135, 677, 685, 709
LmnI GCTCC 2 cut(s) 210, 859
Lsp1109I GCAGC 2 cut(s) 346, 742
LweI GCATC 1 cut(s) 370
MaeI CTAG 1 cut(s) 672
MaeII ACGT 1 cut(s) 129
MalI GATC 5 cut(s) 24, 137, 679, 687, 711
MboI GATC 5 cut(s) 22, 135, 677, 685, 709
MboII GAAGA 6 cut(s) 203, 344, 437, 457, 915, 922
MflI RGATCY 2 cut(s) 677, 709
MhlI GDGCHC 2 cut(s) 348, 663
MlsI TGGCCA 1 cut(s) 905
MluCI AATT 2 cut(s) 515, 934
MluNI TGGCCA 1 cut(s) 905
MlyI GAGTC 2 cut(s) 63, 69
MmeI TCCRAC 1 cut(s) 513
Mox20I TGGCCA 1 cut(s) 905
MscI TGGCCA 1 cut(s) 905
MseI TTAA 3 cut(s) 387, 543, 608
MslI CAYNNNNRTG 2 cut(s) 408, 795
Msp20I TGGCCA 1 cut(s) 905
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 901
MvaI CCWGG 1 cut(s) 506
MvnI CGCG 2 cut(s) 446, 459
MwoI GCNNNNNNNGC 3 cut(s) 494, 553, 658
NcoI CCATGG 1 cut(s) 226
NdeII GATC 5 cut(s) 22, 135, 677, 685, 709
NlaIII CATG 7 cut(s) 23, 230, 378, 705, 789, 866, 910
NlaIV GGNNCC 3 cut(s) 523, 679, 711
NspI RCATGY 1 cut(s) 866
NspV TTCGAA 1 cut(s) 512
PctI GAATGC 1 cut(s) 901
PfeI GAWTC 2 cut(s) 371, 845
PkrI GCNGC 2 cut(s) 361, 757
PleI GAGTC 2 cut(s) 63, 68
PpsI GAGTC 2 cut(s) 63, 68
PsiI TTATAA 1 cut(s) 695
Psp6I CCWGG 1 cut(s) 504
PspFI CCCAGC 1 cut(s) 482
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 3 cut(s) 523, 679, 711
PspPI GGNCC 1 cut(s) 522
PsuI RGATCY 2 cut(s) 677, 709
RseI CAYNNNNRTG 2 cut(s) 408, 795
SaqAI TTAA 3 cut(s) 387, 543, 608
SatI GCNGC 2 cut(s) 360, 756
Sau3AI GATC 5 cut(s) 22, 135, 677, 685, 709
Sau96I GGNCC 1 cut(s) 522
SchI GAGTC 2 cut(s) 63, 69
ScrFI CCNGG 1 cut(s) 506
SduI GDGCHC 2 cut(s) 348, 663
SetI ASST 8 cut(s) 132, 174, 207, 282, 507, 549, 625, 840
SfaNI GCATC 1 cut(s) 370
SfuI TTCGAA 1 cut(s) 512
SinI GGWCC 1 cut(s) 522
SmiMI CAYNNNNRTG 2 cut(s) 408, 795
SpeI ACTAGT 1 cut(s) 671
Sse9I AATT 2 cut(s) 515, 934
SsiI CCGC 4 cut(s) 159, 183, 446, 722
SspMI CTAG 1 cut(s) 672
StyD4I CCNGG 1 cut(s) 504
StyI CCWWGG 2 cut(s) 123, 226
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 1 cut(s) 132
TaqI TCGA 4 cut(s) 58, 63, 512, 668
TasI AATT 2 cut(s) 515, 934
TfiI GAWTC 2 cut(s) 371, 845
Tru1I TTAA 3 cut(s) 387, 543, 608
Tru9I TTAA 3 cut(s) 387, 543, 608
TscAI CASTG 1 cut(s) 810
TseI GCWGC 2 cut(s) 359, 755
TspDTI ATGAA 4 cut(s) 25, 363, 860, 923
TspRI CASTG 1 cut(s) 810
VneI GTGCAC 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 522
XceI RCATGY 1 cut(s) 866
XspI CTAG 1 cut(s) 672
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.