Rw6G008530

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000397
Physical Location & Seq
Reverse (-)
14394056 .. 14394970
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008530.1

Sequence Viewer

Length: 915 bp
ATGGACCCAGCAACCCCAAACGACCAAATAGCCTTCGAATTCCCTCCCCTATTCCGACTCTACAAAGACGGACGCGCTGAACGACTTAAGGGCACCGAAACCGTCCCGCCTTCTACCGACCCGACTACCGGAGTCCAATCCAAAGACATCGTCCTCTCACCCGAGTCCGGTCTCGCTGCCCGCATCTACCTCCCCAAACTCTCCGACCCGACCCGGAAACTCCCTTTCCTCCTCTACATCCACGGCGGCGGCTTCGTCATCGAGTCCCCATACTCCCCTATCTACCACACCCACCTCAACTCCCTCACTGCCGAAGCAAAAGTCGTCGCTTTATCCATCCACTACCGCCGCGCCCCGGAGCACCCCCTTCCCGCCGCTTTTGAAGATTCCTGGGCCGCATTTGAATGGGTCGCCGCCCATTCGAGTGGCAACGGGCCGGAAGCCTGGCTGAACGAACACGCGGACTTTAACCGCGTGTTTGTGGCCGGAGACAGCGCCGGTGCCACTCTGGCGCACCACGTGGTGCGTCAGAGTGGAGTGGAGGGAGGTAGTGGGGTTAATATTGCGGGGGTTGTTTTGTTTCATCCGTATTTTCGGAATGAGAGGCCGGATAAGTTGTTGGAGGTGATATATCCAACGGGCGGCGGGTCGGAGGATCTGAAAGTATACCCGGGAAAGGATCCGAAACTGGGGAGCTTGGGAGCTGGAAGAGTGTTGGTGTTTGTGGCGGAGAAGGATTTTCTGAAGGACAGGGGATGGAGTTATTATGAGGCGTTGAAGAAGAGTGGGTGGGGTGGAGTGGTGGAGATTGAGGAGACTGAAGGAGAAGATCATGTGTTTCATTTGTTCGATCCGAGCTGTGAGAAGGCTGTGAGCTTGGTGAAGAAGGTAGCTGCTTTCATGAATCAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0009056 GO:0016787 GO:0016788 GO:0044424 GO:0044444 GO:0044464 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

304

Amino Acids

33.3

Weight (kDa)

5.65

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 53 - 173 7.4e-07 Carboxylesterase family
BD-FAE PF20434 62 - 172 1.5e-11 BD-FAE
Abhydrolase_3 PF07859 77 - 281 8.6e-45 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 92, 500
AccI GTMKAC 1 cut(s) 666
AccII CGCG 4 cut(s) 75, 351, 461, 474
AclWI GGATC 4 cut(s) 663, 674, 687, 845
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 1 cut(s) 38
AcuI CTGAAG 2 cut(s) 764, 840
AcvI CACGTG 1 cut(s) 520
AdeI CACNNNGTG 2 cut(s) 520, 523
AfiI CCNNNNNNNGG 6 cut(s) 128, 167, 355, 641, 676, 689
AflII CTTAAG 1 cut(s) 86
AgsI TTSAA 3 cut(s) 383, 404, 778
AjnI CCWGG 2 cut(s) 389, 443
AluBI AGCT 5 cut(s) 696, 704, 858, 876, 893
AluI AGCT 5 cut(s) 696, 704, 858, 876, 893
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 3 cut(s) 176, 483, 809
AlwI GGATC 4 cut(s) 663, 674, 687, 845
Ama87I CYCGRG 2 cut(s) 161, 670
AoxI GGCC 4 cut(s) 393, 434, 483, 605
ApeKI GCWGC 2 cut(s) 176, 893
ApoI RAATTY 1 cut(s) 38
ArsI GACNNNNNNTTYG 4 cut(s) 135, 167, 306, 338
AspLEI GCGC 4 cut(s) 77, 353, 497, 514
AspS9I GGNCC 3 cut(s) 4, 393, 434
AsuC2I CCSGG 4 cut(s) 214, 356, 671, 672
AsuHPI GGTGA 3 cut(s) 150, 637, 892
AsuII TTCGAA 1 cut(s) 36
AvaI CYCGRG 2 cut(s) 161, 670
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 95
BamHI GGATCC 1 cut(s) 679
BanI GGYRCC 2 cut(s) 92, 500
BbrPI CACGTG 1 cut(s) 520
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 163, 880
BccI CCATC 2 cut(s) 344, 750
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 2 cut(s) 391, 445
BcnI CCSGG 4 cut(s) 214, 356, 671, 672
BcoDI GTCTC 3 cut(s) 176, 483, 809
BfoI RGCGCY 1 cut(s) 498
BfrI CTTAAG 1 cut(s) 86
BglI GCCNNNNNGGC 1 cut(s) 509
BisI GCNGC 9 cut(s) 177, 247, 250, 349, 375, 396, 414, 643, 894
BlsI GCNGC 9 cut(s) 178, 248, 251, 350, 376, 397, 415, 644, 895
Bme1390I CCNGG 6 cut(s) 214, 356, 391, 445, 671, 672
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 2 cut(s) 161, 670
BmgT120I GGNCC 3 cut(s) 4, 393, 434
BmiI GGNNCC 4 cut(s) 6, 94, 502, 681
BmrFI CCNGG 6 cut(s) 214, 356, 391, 445, 671, 672
BmrI ACTGGG 1 cut(s) 698
BmsI GCATC 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 698
Bpu14I TTCGAA 1 cut(s) 36
BpuMI CCSGG 4 cut(s) 214, 356, 671, 672
BsaAI YACGTR 1 cut(s) 520
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 4 cut(s) 241, 354, 390, 670
BsaWI WCCGGW 2 cut(s) 128, 167
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 167, 355, 641, 676, 689
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 693
BseBI CCWGG 2 cut(s) 391, 445
BseDI CCNNGG 4 cut(s) 241, 354, 390, 670
BseGI GGATG 4 cut(s) 237, 336, 583, 761
BseLI CCNNNNNNNGG 6 cut(s) 128, 167, 355, 641, 676, 689
BseNI ACTGG 1 cut(s) 693
BseRI GAGGAG 2 cut(s) 221, 827
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 2 cut(s) 163, 880
BseYI CCCAGC 1 cut(s) 7
Bsh1236I CGCG 4 cut(s) 75, 351, 461, 474
BshFI GGCC 4 cut(s) 395, 436, 485, 607
BshNI GGYRCC 2 cut(s) 92, 500
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 2 cut(s) 161, 670
BsiSI CCGG 9 cut(s) 129, 168, 214, 356, 437, 486, 498, 608, 671
BslFI GGGAC 2 cut(s) 89, 250
BslI CCNNNNNNNGG 6 cut(s) 128, 167, 355, 641, 676, 689
BsmAI GTCTC 3 cut(s) 176, 483, 809
BsmFI GGGAC 2 cut(s) 89, 250
BsnI GGCC 4 cut(s) 395, 436, 485, 607
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 2 cut(s) 161, 670
Bsp119I TTCGAA 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 95, 363
Bsp143I GATC 4 cut(s) 655, 679, 829, 850
BspANI GGCC 4 cut(s) 395, 436, 485, 607
BspFNI CGCG 4 cut(s) 75, 351, 461, 474
BspHI TCATGA 1 cut(s) 900
BspLI GGNNCC 4 cut(s) 6, 94, 502, 681
BspPI GGATC 4 cut(s) 663, 674, 687, 845
BspT104I TTCGAA 1 cut(s) 36
BspT107I GGYRCC 2 cut(s) 92, 500
BspTI CTTAAG 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 1 cut(s) 693
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 4 cut(s) 241, 354, 390, 670
BssMI GATC 4 cut(s) 655, 679, 829, 850
BssNAI GTATAC 1 cut(s) 667
Bst1107I GTATAC 1 cut(s) 667
Bst2UI CCWGG 2 cut(s) 391, 445
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 2 cut(s) 703, 776
BstAFI CTTAAG 1 cut(s) 86
BstBAI YACGTR 1 cut(s) 520
BstBI TTCGAA 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 181
BstDSI CCRYGG 1 cut(s) 241
BstF5I GGATG 4 cut(s) 237, 336, 583, 761
BstFNI CGCG 4 cut(s) 75, 351, 461, 474
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 4 cut(s) 77, 353, 497, 514
BstKTI GATC 4 cut(s) 658, 682, 832, 853
BstMAI GTCTC 3 cut(s) 176, 483, 809
BstMBI GATC 4 cut(s) 655, 679, 829, 850
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNI CCWGG 2 cut(s) 391, 445
BstSCI CCNGG 6 cut(s) 212, 354, 389, 443, 669, 670
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 4 cut(s) 75, 351, 461, 474
BstV1I GCAGC 2 cut(s) 163, 880
BstX2I RGATCY 2 cut(s) 655, 679
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 2 cut(s) 655, 679
BstZ17I GTATAC 1 cut(s) 667
BsuRI GGCC 4 cut(s) 395, 436, 485, 607
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 4 cut(s) 237, 336, 583, 761
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 1 cut(s) 181
CciI TCATGA 1 cut(s) 900
CfoI GCGC 4 cut(s) 77, 353, 497, 514
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 3 cut(s) 4, 393, 434
Cfr9I CCCGGG 1 cut(s) 670
CseI GACGC 2 cut(s) 81, 515
CviAII CATG 2 cut(s) 833, 901
DpnI GATC 4 cut(s) 657, 681, 831, 852
DpnII GATC 4 cut(s) 655, 679, 829, 850
DraIII CACNNNGTG 2 cut(s) 520, 523
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 2 cut(s) 703, 776
EarI CTCTTC 2 cut(s) 703, 776
EciI GGCGGA 1 cut(s) 743
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 2 cut(s) 764, 840
Eco72I CACGTG 1 cut(s) 520
Eco88I CYCGRG 2 cut(s) 161, 670
EcoRI GAATTC 1 cut(s) 38
EcoRII CCWGG 2 cut(s) 389, 443
FaeI CATG 2 cut(s) 836, 904
FaiI YATR 6 cut(s) 271, 631, 667, 768, 834, 902
FaqI GGGAC 2 cut(s) 89, 250
FatI CATG 2 cut(s) 832, 900
FauI CCCGC 5 cut(s) 114, 188, 379, 559, 638
FblI GTMKAC 1 cut(s) 666
Fnu4HI GCNGC 9 cut(s) 177, 247, 250, 349, 375, 396, 414, 643, 894
FokI GGATG 4 cut(s) 224, 323, 570, 768
Fsp4HI GCNGC 9 cut(s) 177, 247, 250, 349, 375, 396, 414, 643, 894
GlaI GCGC 4 cut(s) 76, 352, 496, 513
GluI GCNGC 9 cut(s) 177, 247, 250, 349, 375, 396, 414, 643, 894
GsaI CCCAGC 1 cut(s) 11
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 4 cut(s) 395, 436, 485, 607
HapII CCGG 9 cut(s) 129, 168, 214, 356, 437, 486, 498, 608, 671
HgaI GACGC 2 cut(s) 81, 515
HhaI GCGC 4 cut(s) 77, 353, 497, 514
Hin1II CATG 2 cut(s) 836, 904
Hin6I GCGC 4 cut(s) 75, 351, 495, 512
HinP1I GCGC 4 cut(s) 75, 351, 495, 512
HinfI GANTC 6 cut(s) 57, 132, 164, 263, 386, 904
HpaII CCGG 9 cut(s) 129, 168, 214, 356, 437, 486, 498, 608, 671
HphI GGTGA 3 cut(s) 150, 637, 892
Hpy166II GTNNAC 1 cut(s) 667
Hpy188I TCNGA 9 cut(s) 56, 205, 531, 597, 652, 660, 684, 744, 855
Hpy188III TCNNGA 2 cut(s) 901, 908
Hpy8I GTNNAC 1 cut(s) 667
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 8 cut(s) 43, 120, 377, 727, 739, 815, 859, 880
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 1 cut(s) 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpySE526I ACGT 1 cut(s) 519
Hsp92II CATG 2 cut(s) 836, 904
HspAI GCGC 4 cut(s) 75, 351, 495, 512
Kzo9I GATC 4 cut(s) 655, 679, 829, 850
LmnI GCTCC 3 cut(s) 358, 693, 701
Lsp1109I GCAGC 2 cut(s) 163, 880
LweI GCATC 1 cut(s) 192
MaeII ACGT 1 cut(s) 519
MalI GATC 4 cut(s) 657, 681, 831, 852
MboI GATC 4 cut(s) 655, 679, 829, 850
MboII GAAGA 6 cut(s) 395, 720, 790, 793, 839, 895
MflI RGATCY 2 cut(s) 655, 679
MhlI GDGCHC 2 cut(s) 95, 363
MluCI AATT 1 cut(s) 38
MlyI GAGTC 4 cut(s) 51, 141, 173, 272
MmeI TCCRAC 5 cut(s) 79, 228, 600, 630, 659
MseI TTAA 4 cut(s) 87, 468, 558, 913
MslI CAYNNNNRTG 2 cut(s) 403, 423
MspCI CTTAAG 1 cut(s) 86
MspI CCGG 9 cut(s) 129, 168, 214, 356, 437, 486, 498, 608, 671
MspR9I CCNGG 6 cut(s) 214, 356, 391, 445, 671, 672
MvaI CCWGG 2 cut(s) 391, 445
MvnI CGCG 4 cut(s) 75, 351, 461, 474
MwoI GCNNNNNNNGC 1 cut(s) 509
NciI CCSGG 4 cut(s) 214, 356, 671, 672
NdeII GATC 4 cut(s) 655, 679, 829, 850
NlaIII CATG 2 cut(s) 836, 904
NlaIV GGNNCC 4 cut(s) 6, 94, 502, 681
NspV TTCGAA 1 cut(s) 36
PagI TCATGA 1 cut(s) 900
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PfeI GAWTC 2 cut(s) 386, 904
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 9 cut(s) 178, 248, 251, 350, 376, 397, 415, 644, 895
PleI GAGTC 4 cut(s) 51, 140, 172, 271
PmaCI CACGTG 1 cut(s) 520
PmlI CACGTG 1 cut(s) 520
PpsI GAGTC 4 cut(s) 51, 140, 172, 271
Ppu21I YACGTR 1 cut(s) 520
Psp6I CCWGG 2 cut(s) 389, 443
PspCI CACGTG 1 cut(s) 520
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 2 cut(s) 389, 443
PspN4I GGNNCC 4 cut(s) 6, 94, 502, 681
PspPI GGNCC 3 cut(s) 4, 393, 434
PsuI RGATCY 2 cut(s) 655, 679
PsyI GACNNNGTC 1 cut(s) 149
RseI CAYNNNNRTG 2 cut(s) 403, 423
SaqAI TTAA 4 cut(s) 87, 468, 558, 913
SatI GCNGC 9 cut(s) 177, 247, 250, 349, 375, 396, 414, 643, 894
Sau3AI GATC 4 cut(s) 655, 679, 829, 850
Sau96I GGNCC 3 cut(s) 4, 393, 434
SchI GAGTC 4 cut(s) 51, 141, 173, 272
ScrFI CCNGG 6 cut(s) 214, 356, 391, 445, 671, 672
SduI GDGCHC 2 cut(s) 95, 363
SfaNI GCATC 1 cut(s) 192
SfuI TTCGAA 1 cut(s) 36
SgrAI CRCCGGYG 1 cut(s) 497
SinI GGWCC 1 cut(s) 4
SmaI CCCGGG 1 cut(s) 672
SmiMI CAYNNNNRTG 2 cut(s) 403, 423
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 1 cut(s) 38
SspI AATATT 1 cut(s) 562
StyD4I CCNGG 6 cut(s) 212, 354, 389, 443, 669, 670
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 1 cut(s) 522
TaqI TCGA 4 cut(s) 36, 261, 422, 849
TasI AATT 1 cut(s) 38
TauI GCSGC 7 cut(s) 249, 252, 351, 377, 398, 416, 645
TfiI GAWTC 2 cut(s) 386, 904
Tru1I TTAA 4 cut(s) 87, 468, 558, 913
Tru9I TTAA 4 cut(s) 87, 468, 558, 913
TscAI CASTG 1 cut(s) 313
TseI GCWGC 2 cut(s) 176, 893
TspDTI ATGAA 3 cut(s) 572, 830, 889
TspGWI ACGGA 2 cut(s) 84, 576
TspMI CCCGGG 1 cut(s) 670
TspRI CASTG 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 149
Vha464I CTTAAG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 38
XmaI CCCGGG 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.