Rroxscaffold_7G00205650

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011520
Physical Location & Seq
Forward (+)
55209428 .. 55213247
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205650.1

Sequence Viewer

Length: 2835 bp
ATGGAGTTGGCTGCAGCCATGGCCGGCATTTTGAGTTGTGTATGTCCCAAAAATGTTATACAAGAAACAACAGGGCAGCATTGCATGTTTCTTCATTACAATGCAAAAGCAAAGATAGATGAGTTGAGGTCGAAAATGGAGGAATTAAGAGCACTTGTGGCGGATGTAAAAAGAAAACTGGAGGATACATGTCTCCAAGAAGGAAAGGAACCCACAGAACAGGTGAAGTTGTGGTTGAGAAATGTTCAAAGTTTTGATAATGAAAAGCTGAATAAACTACTATCTTGGGAAGAGGAAAGATGTTTATGTGGGTGTCTTCCAGATTATTACTCTCGAATGAAGTTGGGGAAACTTGTCGAAAGGAGGATTCAGGAGGTGGATGAGCTTCTCAGGAGAGGACAGTTTCAAGATGATGCATTGGCTCACTTGCTGTTAGAGAATATAAAGATGCTTCCTATGACGGTGGCGGTGGGTAATGAAACAACTAAAAGAAAGTTGGAAGAGATATGGGAGCATTTAATGGCAGATTATGAAGTGCAAAGAATTGGTGTTTATGGAATGGGTGGGGTTGGAAAAACAACAATTCTGAAAGAAATAAATAACAGACTCAGAAAAGAGATGACAGAAGGGACGATCACTTGCTTTGACAATGTCGTTTGGGTCACGATACCGAAGGAGTCCAACCCGGAGAAATTGCAAATGGCTATTGCCAAAGCCGTGAGGTTGAATCTCCCTGAAGATTGGAATATGATGGACAAGGCAGCAGAGTTGCTGAAAACATTACAACGAAGGAAGAGGCTTTTGCTAATTTTTGATGATGTATGGACTCCATTTTCATTGGAAGATATTGGAATTCCTGCACCTTCCAGGGCCAATGGCTGCAAACTCATATTAACTACTAGATCATTAAAGGTGTGTCGAGGCATGGAGACAAACAAAGAAGTGGAGGTAAAAGTTCTTTCTGAGGAAGAATCTTGGACCCTTTTTACTGATAAAGTTGGTTCTGTTGTGTTACAAAATCCCGAAATTAAAATTATTGCAAGGGAAATAGCAAAAGAATTTGGTGGTCTTCCACTAGCCATTATCACAGTTGGAAGAGCCTTGAGAAAAGTAAGTAATGAAATTGAGTGGCGAAATGCATTGACAGCCTTAAGAGGTTCTGCAGCTAATGTGGAAGGCATGGAAGAGCTGGTGTATTCTCGTTTGAGATTCAGTTATGAAAGATTAAAGGATGACACTACTCGATCATGTTTTCTTTATTGTGCATTGTATCCTGAAGATCATCAAATTGAAGCCAAAGAGCTAATAGAATATTGGATTTGGGAGGGTTTGCTTGGTGATGGGGGAAGGATACAGGCCAACATATGGAAGGGAGAAATGATTTTGGCTGATCTTAAAGATTCTTGCCTGCTGGACAATGTTGATGTAGATGGCGGTGTTGAATGTGTCAAAATGCATGACTTAATAAGGGATATGGCTATTGGTATTACAAATATCAGTCCTCGGTTTGTCATTAGTGCTGGGGTCGGAGTAGAAAAGGCATCACTTGGTGAGGAGGGGATTGAAGATGTTGAGAGGATTTCAATGATGTATAATGAATTAAAATGCCTTCCTAGCCAACCATTGTGTTCCAAGCTCTCCTCTTTGCTGCTGCAGTATAGCTCGATTGCCAATGATATCTCACATTGTTTCTTCTGCCACATGCCAAAGCTCAGAGTACTTGATCTGTCTTTCACTGGAATTCTTTGTATACCTGACTCCATTGCTGAACTAAGCAATTTGCGTGCACTGCTATTGCGTAGTTGTTGGAACCTAAGTAGTCTTCCTTCTCTAGCAACACTCGAGGCATTGTGGGTTTTGGATCTTTCATATACTCACATCACAGAATTGCCTCTAGGTATGGAAAAGTTACGCCAGCTTAGGCGCCTCAATCTTTCTTATACTAAATTGGACAGTTTTCCTAAAGGACTGTTAACCACTCTCAATCTGCTAGAAGAATTGCTCCTATATCGTTGTAAGTGTGAATTTGGTTTACAGTTTGTTGATGAACTCATCACCTCAAGAAATATTGTTGTTCTTGAAGTCGAATTCTCAACCTCCAGAGTGTTTGATACGTATACAAGATCAGGGCATTGGAATCTTCTGCGGAGTTTTAGATCCGGCATATGCTCATATGGCGATGACTTGGGAAAGAACAGTATAGACTACATTGGAAATTTTTTTCTTAGTGGTACTCCTGTTTTGCTCCCTGATCGCACCTATGAACTGCGACTCCTCCACTGCTATGATGTTGATCGATTGTCAAGGTGCTTGTCAGAATCAATGAAATTGAAAAAATGTGTCATAACACACTGCGATCAAATGGAGAGTATACTAATGACTGGGGAGAACAACTTAAGCTGTCTAAATACGTTAGAGCTTGGTTTCCTACAAAACTTTAAGAGTCTCTACAAGGGTGTCATGCCCTCAGGAGCTCTTGGTGCCCTGAAGATACTGCAAATCAGTTATTGTAACTCTTTAGAGAGCGTACTCAACTCAAGGTTGATTGAACACCTCAGAAACCTCGAAGAACTTTCTGTTGAACATTGCCCAAAATTGGAGGTGGTGATAGAGGAGGAGCAGTCAATCCACAATGCTGAAGTCACCTTTTCAAGATTGAAGAGCTTAAAGTTGTGTGAATTAAGGGAACTGGAGAGCATTTATAGTGAAGAAGTAATTTGCATATCTCTATGCACCATCACTATTGAGGGATGCAATAAACTGAGGAGACTTCCTATTAGGATTTCGATAGATGAAGGATATCAGCAAAGACAACCATCAATCACTCCCCCATAA

Protein Analysis

944

Amino Acids

107.5

Weight (kDa)

5.54

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 164 - 334 5.7e-42 NB-ARC domain
TniB PF05621 165 - 274 1.1e-06 Bacterial TniB protein
WHD_DRP PF23559 423 - 493 2e-10 Disease resistance protein Winged helix domain
LRR_14 PF23598 569 - 699 1.6e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 814 - 924 1e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1923, 2480
AccB7I CCANNNNNTGG 1 cut(s) 1365
AccI GTMKAC 3 cut(s) 1750, 2117, 2371
AciI CCGC 4 cut(s) 161, 467, 1434, 2146
AclWI GGATC 2 cut(s) 1869, 2151
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 6 cut(s) 852, 1058, 1740, 2024, 2087, 2215
AcuI CTGAAG 4 cut(s) 756, 1296, 2507, 2658
AcyI GRCGYC 1 cut(s) 1924
AdeI CACNNNGTG 1 cut(s) 1550
AfaI GTAC 3 cut(s) 1719, 2233, 2529
AfiI CCNNNNNNNGG 2 cut(s) 1365, 2596
AflII CTTAAG 2 cut(s) 1150, 2395
AflIII ACRYGT 1 cut(s) 188
AjnI CCWGG 1 cut(s) 866
AloI GAACNNNNNNTCC 2 cut(s) 2256, 2288
Alw21I GWGCWC 3 cut(s) 154, 1789, 2476
Alw26I GTCTC 4 cut(s) 197, 923, 2450, 2761
Alw44I GTGCAC 1 cut(s) 1785
AlwI GGATC 2 cut(s) 1869, 2151
Ama87I CYCGRG 1 cut(s) 1841
AoxI GGCC 3 cut(s) 21, 870, 1356
ApaLI GTGCAC 1 cut(s) 1785
ApeKI GCWGC 8 cut(s) 11, 14, 76, 761, 879, 1163, 1648, 1651
ApoI RAATTY 6 cut(s) 852, 1058, 1740, 2024, 2087, 2215
AspLEI GCGC 1 cut(s) 1926
AspS9I GGNCC 2 cut(s) 870, 978
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 6 cut(s) 235, 1349, 1562, 2047, 2617, 2635
AvaI CYCGRG 1 cut(s) 1841
AvaII GGWCC 1 cut(s) 978
AxyI CCTNAGG 1 cut(s) 2467
BaeGI GKGCMC 2 cut(s) 1789, 2485
BaeI ACNNNNGTAYC 2 cut(s) 2223, 2256
BanI GGYRCC 2 cut(s) 1923, 2480
BanII GRGCYC 1 cut(s) 2476
BarI GAAGNNNNNNTAC 2 cut(s) 1810, 1842
BbsI GAAGAC 3 cut(s) 308, 1061, 1814
Bbv12I GWGCWC 3 cut(s) 154, 1789, 2476
BbvI GCAGC 7 cut(s) 26, 88, 773, 866, 1175, 1635, 1638
BccI CCATC 5 cut(s) 745, 1334, 1424, 2744, 2824
BceAI ACGGC 1 cut(s) 701
BciT130I CCWGG 1 cut(s) 868
BciVI GTATCC 3 cut(s) 178, 1281, 1344
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 4 cut(s) 197, 923, 2450, 2761
BfaI CTAG 6 cut(s) 900, 1076, 1614, 1832, 1895, 1991
BfmI CTRYAG 3 cut(s) 12, 1161, 1652
BfoI RGCGCY 1 cut(s) 1927
BfrI CTTAAG 2 cut(s) 1150, 2395
BfuI GTATCC 3 cut(s) 178, 1281, 1344
BisI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
BlsI GCNGC 8 cut(s) 13, 16, 78, 763, 881, 1165, 1650, 1653
BmcAI AGTACT 1 cut(s) 1719
Bme1390I CCNGG 2 cut(s) 686, 868
Bme18I GGWCC 1 cut(s) 978
BmeT110I CYCGRG 1 cut(s) 1841
BmgT120I GGNCC 2 cut(s) 870, 978
BmiI GGNNCC 5 cut(s) 210, 980, 1811, 1925, 2482
BmrFI CCNGG 2 cut(s) 686, 868
BmrI ACTGGG 1 cut(s) 2391
BmsI GCATC 4 cut(s) 403, 438, 1550, 2741
BmuI ACTGGG 1 cut(s) 2391
BpiI GAAGAC 3 cut(s) 308, 1061, 1814
BpmI CTGGAG 3 cut(s) 200, 2083, 2711
Bpu10I CCTNAGC 1 cut(s) 1919
BpuEI CTTGAG 3 cut(s) 1123, 2044, 2521
BpuMI CCSGG 1 cut(s) 686
Bsa29I ATCGAT 1 cut(s) 2296
BsaAI YACGTR 1 cut(s) 2115
BsaBI GATNNNNATC 1 cut(s) 2292
BsaHI GRCGYC 1 cut(s) 1924
BsaJI CCNNGG 3 cut(s) 18, 867, 1502
BsaXI ACNNNNNCTCC 4 cut(s) 2256, 2286, 2606, 2636
Bsc4I CCNNNNNNNGG 2 cut(s) 1365, 2596
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 4 cut(s) 183, 1741, 2386, 2694
Bse21I CCTNAGG 1 cut(s) 2467
Bse3DI GCAATG 3 cut(s) 79, 1761, 2584
Bse8I GATNNNNATC 1 cut(s) 2292
BseBI CCWGG 1 cut(s) 868
BseCI ATCGAT 1 cut(s) 2296
BseDI CCNNGG 3 cut(s) 18, 867, 1502
BseGI GGATG 4 cut(s) 169, 385, 1237, 2756
BseJI GATNNNNATC 1 cut(s) 2292
BseLI CCNNNNNNNGG 2 cut(s) 1365, 2596
BseMI GCAATG 3 cut(s) 79, 1761, 2584
BseMII CTCAG 7 cut(s) 403, 622, 954, 1726, 2481, 2569, 2753
BseNI ACTGG 4 cut(s) 183, 1741, 2386, 2694
BseRI GAGGAG 6 cut(s) 1568, 1630, 2264, 2627, 2630, 2779
BseSI GKGCMC 2 cut(s) 1789, 2485
BseXI GCAGC 7 cut(s) 26, 88, 773, 866, 1175, 1635, 1638
BseYI CCCAGC 1 cut(s) 1520
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 3 cut(s) 23, 872, 1358
BshNI GGYRCC 2 cut(s) 1923, 2480
BshVI ATCGAT 1 cut(s) 2296
BsiHKAI GWGCWC 3 cut(s) 154, 1789, 2476
BsiHKCI CYCGRG 1 cut(s) 1841
BsiSI CCGG 3 cut(s) 24, 686, 2160
BslFI GGGAC 2 cut(s) 30, 643
BslI CCNNNNNNNGG 2 cut(s) 1365, 2596
BsmAI GTCTC 4 cut(s) 197, 923, 2450, 2761
BsmFI GGGAC 2 cut(s) 30, 643
BsnI GGCC 3 cut(s) 23, 872, 1358
BsoBI CYCGRG 1 cut(s) 1841
Bsp1286I GDGCHC 4 cut(s) 154, 1789, 2476, 2485
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 4 cut(s) 161, 467, 1434, 2146
BspANI GGCC 3 cut(s) 23, 872, 1358
BspCNI CTCAG 7 cut(s) 402, 621, 955, 1725, 2480, 2568, 2754
BspDI ATCGAT 1 cut(s) 2296
BspLI GGNNCC 5 cut(s) 210, 980, 1811, 1925, 2482
BspMAI CTGCAG 3 cut(s) 16, 1165, 1656
BspPI GGATC 2 cut(s) 1869, 2151
BspQI GCTCTTC 3 cut(s) 1090, 1179, 2654
BspT107I GGYRCC 2 cut(s) 1923, 2480
BspTI CTTAAG 2 cut(s) 1150, 2395
BsrDI GCAATG 3 cut(s) 79, 1761, 2584
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 4 cut(s) 183, 1741, 2386, 2694
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 3 cut(s) 18, 867, 1502
BssNAI GTATAC 3 cut(s) 1751, 2118, 2372
BssNI GRCGYC 1 cut(s) 1924
BssT1I CCWWGG 1 cut(s) 18
Bst1107I GTATAC 3 cut(s) 1751, 2118, 2372
Bst2UI CCWGG 1 cut(s) 868
Bst4CI ACNGT 7 cut(s) 402, 463, 1090, 1955, 1971, 2037, 2198
Bst6I CTCTTC 6 cut(s) 285, 495, 788, 1090, 1179, 2654
BstACI GRCGYC 1 cut(s) 1924
BstAFI CTTAAG 2 cut(s) 1150, 2395
BstBAI YACGTR 1 cut(s) 2115
BstC8I GCNNGC 4 cut(s) 25, 1409, 1785, 1916
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 4 cut(s) 169, 385, 1237, 2756
BstH2I RGCGCY 1 cut(s) 1927
BstHHI GCGC 1 cut(s) 1926
BstMAI GTCTC 4 cut(s) 197, 923, 2450, 2761
BstMWI GCNNNNNNNGC 7 cut(s) 20, 158, 1145, 1614, 1789, 2175, 2480
BstNI CCWGG 1 cut(s) 868
BstNSI RCATGY 3 cut(s) 88, 192, 1705
BstSCI CCNGG 2 cut(s) 684, 866
BstSFI CTRYAG 3 cut(s) 12, 1161, 1652
BstSLI GKGCMC 2 cut(s) 1789, 2485
BstSNI TACGTA 1 cut(s) 2115
BstV1I GCAGC 7 cut(s) 26, 88, 773, 866, 1175, 1635, 1638
BstV2I GAAGAC 3 cut(s) 308, 1061, 1814
BstX2I RGATCY 2 cut(s) 1861, 2156
BstYI RGATCY 2 cut(s) 1861, 2156
BstZ17I GTATAC 3 cut(s) 1751, 2118, 2372
Bsu15I ATCGAT 1 cut(s) 2296
Bsu36I CCTNAGG 1 cut(s) 2467
BsuI GTATCC 3 cut(s) 178, 1281, 1344
BsuRI GGCC 3 cut(s) 23, 872, 1358
BsuTUI ATCGAT 1 cut(s) 2296
BtgI CCRYGG 1 cut(s) 18
BtgZI GCGATG 1 cut(s) 2193
BtsCI GGATG 4 cut(s) 169, 385, 1237, 2756
BtsI GCAGTG 3 cut(s) 1787, 2278, 2350
BtsIMutI CAGTG 4 cut(s) 1734, 1787, 2278, 2350
Cac8I GCNNGC 4 cut(s) 25, 1409, 1785, 1916
CfoI GCGC 1 cut(s) 1926
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 870, 978
ClaI ATCGAT 1 cut(s) 2296
Csp6I GTAC 3 cut(s) 1718, 2232, 2528
CviAII CATG 9 cut(s) 19, 85, 189, 925, 1180, 1248, 1457, 1702, 2461
CviQI GTAC 3 cut(s) 1718, 2232, 2528
DinI GGCGCC 1 cut(s) 1925
DraIII CACNNNGTG 1 cut(s) 1550
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 6 cut(s) 285, 495, 788, 1090, 1179, 2654
EarI CTCTTC 6 cut(s) 285, 495, 788, 1090, 1179, 2654
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 2474
Eco105I TACGTA 1 cut(s) 2115
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 2476
Eco32I GATATC 2 cut(s) 1678, 2801
Eco47I GGWCC 1 cut(s) 978
Eco53kI GAGCTC 1 cut(s) 2474
Eco57I CTGAAG 4 cut(s) 756, 1296, 2507, 2658
Eco81I CCTNAGG 1 cut(s) 2467
Eco88I CYCGRG 1 cut(s) 1841
EcoICRI GAGCTC 1 cut(s) 2474
EcoRI GAATTC 3 cut(s) 852, 1740, 2087
EcoRII CCWGG 1 cut(s) 866
EcoRV GATATC 2 cut(s) 1678, 2801
EcoT14I CCWWGG 1 cut(s) 18
EcoT22I ATGCAT 3 cut(s) 418, 1141, 1458
EcoT38I GRGCYC 1 cut(s) 2476
EgeI GGCGCC 1 cut(s) 1925
EheI GGCGCC 1 cut(s) 1925
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 9 cut(s) 22, 88, 192, 928, 1183, 1251, 1460, 1705, 2464
FaqI GGGAC 2 cut(s) 30, 643
FatI CATG 9 cut(s) 18, 84, 188, 924, 1179, 1247, 1456, 1701, 2460
FauNDI CATATG 3 cut(s) 1364, 2165, 2173
FblI GTMKAC 3 cut(s) 1750, 2117, 2371
Fnu4HI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
FokI GGATG 4 cut(s) 176, 392, 1244, 2763
FriOI GRGCYC 1 cut(s) 2476
Fsp4HI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
FspBI CTAG 6 cut(s) 900, 1076, 1614, 1832, 1895, 1991
GlaI GCGC 1 cut(s) 1925
GluI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
GsaI CCCAGC 1 cut(s) 1524
GsuI CTGGAG 3 cut(s) 200, 2083, 2711
HaeII RGCGCY 1 cut(s) 1927
HaeIII GGCC 3 cut(s) 23, 872, 1358
HapII CCGG 3 cut(s) 24, 686, 2160
HhaI GCGC 1 cut(s) 1926
Hin1I GRCGYC 1 cut(s) 1924
Hin1II CATG 9 cut(s) 22, 88, 192, 928, 1183, 1251, 1460, 1705, 2464
Hin6I GCGC 1 cut(s) 1924
HinP1I GCGC 1 cut(s) 1924
HincII GTYRAC 1 cut(s) 1974
HindII GTYRAC 1 cut(s) 1974
HpaI GTTAAC 1 cut(s) 1974
HpaII CCGG 3 cut(s) 24, 686, 2160
HphI GGTGA 6 cut(s) 235, 1349, 1562, 2047, 2617, 2635
Hpy166II GTNNAC 6 cut(s) 1751, 1787, 1974, 2033, 2118, 2372
Hpy188I TCNGA 7 cut(s) 588, 611, 964, 1529, 1715, 2317, 2558
Hpy8I GTNNAC 6 cut(s) 1751, 1787, 1974, 2033, 2118, 2372
HpyCH4III ACNGT 7 cut(s) 402, 463, 1090, 1955, 1971, 2037, 2198
HpyCH4IV ACGT 2 cut(s) 2114, 2411
HpyF10VI GCNNNNNNNGC 7 cut(s) 20, 158, 1145, 1614, 1789, 2175, 2480
HpySE526I ACGT 2 cut(s) 2114, 2411
Hsp92I GRCGYC 1 cut(s) 1924
Hsp92II CATG 9 cut(s) 22, 88, 192, 928, 1183, 1251, 1460, 1705, 2464
HspAI GCGC 1 cut(s) 1924
KasI GGCGCC 1 cut(s) 1923
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
KspAI GTTAAC 1 cut(s) 1974
LguI GCTCTTC 3 cut(s) 1090, 1179, 2654
LmnI GCTCC 5 cut(s) 511, 2007, 2250, 2471, 2617
Lsp1109I GCAGC 7 cut(s) 26, 88, 773, 866, 1175, 1635, 1638
LweI GCATC 4 cut(s) 403, 438, 1550, 2741
MaeI CTAG 6 cut(s) 900, 1076, 1614, 1832, 1895, 1991
MaeII ACGT 2 cut(s) 2114, 2411
MaeIII GTNAC 5 cut(s) 661, 1011, 1908, 2510, 2641
MflI RGATCY 2 cut(s) 1861, 2156
MhlI GDGCHC 4 cut(s) 154, 1789, 2476, 2485
Mly113I GGCGCC 1 cut(s) 1924
MlyI GAGTC 6 cut(s) 600, 686, 820, 1751, 2265, 2452
MmeI TCCRAC 6 cut(s) 477, 550, 705, 1072, 1507, 1787
Mph1103I ATGCAT 3 cut(s) 418, 1141, 1458
MroNI GCCGGC 1 cut(s) 23
MslI CAYNNNNRTG 2 cut(s) 99, 2283
MspCI CTTAAG 2 cut(s) 1150, 2395
MspI CCGG 3 cut(s) 24, 686, 2160
MspR9I CCNGG 2 cut(s) 686, 868
MvaI CCWGG 1 cut(s) 868
MwoI GCNNNNNNNGC 7 cut(s) 20, 158, 1145, 1614, 1789, 2175, 2480
NaeI GCCGGC 1 cut(s) 25
NarI GGCGCC 1 cut(s) 1924
NciI CCSGG 1 cut(s) 686
NcoI CCATGG 1 cut(s) 18
NdeI CATATG 3 cut(s) 1364, 2165, 2173
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 9 cut(s) 22, 88, 192, 928, 1183, 1251, 1460, 1705, 2464
NlaIV GGNNCC 5 cut(s) 210, 980, 1811, 1925, 2482
NmuCI GTSAC 2 cut(s) 661, 2641
NsiI ATGCAT 3 cut(s) 418, 1141, 1458
NspI RCATGY 3 cut(s) 88, 192, 1705
PaeR7I CTCGAG 1 cut(s) 1841
PciI ACATGT 1 cut(s) 188
PciSI GCTCTTC 3 cut(s) 1090, 1179, 2654
PdiI GCCGGC 1 cut(s) 25
PfeI GAWTC 7 cut(s) 367, 727, 971, 1209, 1400, 2137, 2318
PflFI GACNNNGTC 1 cut(s) 650
PflMI CCANNNNNTGG 1 cut(s) 1365
PkrI GCNGC 8 cut(s) 13, 16, 78, 763, 881, 1165, 1650, 1653
PleI GAGTC 6 cut(s) 600, 685, 820, 1751, 2265, 2451
PluTI GGCGCC 1 cut(s) 1927
PpsI GAGTC 6 cut(s) 600, 685, 820, 1751, 2265, 2451
Ppu21I YACGTR 1 cut(s) 2115
PscI ACATGT 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 2476
Psp6I CCWGG 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 1520
PspGI CCWGG 1 cut(s) 866
PspN4I GGNNCC 5 cut(s) 210, 980, 1811, 1925, 2482
PspPI GGNCC 2 cut(s) 870, 978
PspXI VCTCGAGB 1 cut(s) 1841
PstI CTGCAG 3 cut(s) 16, 1165, 1656
PsuI RGATCY 2 cut(s) 1861, 2156
PsyI GACNNNGTC 1 cut(s) 650
RsaI GTAC 3 cut(s) 1719, 2233, 2529
RsaNI GTAC 3 cut(s) 1718, 2232, 2528
RseI CAYNNNNRTG 2 cut(s) 99, 2283
SacI GAGCTC 1 cut(s) 2476
SapI GCTCTTC 3 cut(s) 1090, 1179, 2654
SatI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
Sau96I GGNCC 2 cut(s) 870, 978
ScaI AGTACT 1 cut(s) 1719
SchI GAGTC 6 cut(s) 600, 686, 820, 1751, 2265, 2452
ScrFI CCNGG 2 cut(s) 686, 868
SduI GDGCHC 4 cut(s) 154, 1789, 2476, 2485
SfaNI GCATC 4 cut(s) 403, 438, 1550, 2741
SfcI CTRYAG 3 cut(s) 12, 1161, 1652
SfoI GGCGCC 1 cut(s) 1925
Sfr274I CTCGAG 1 cut(s) 1841
SinI GGWCC 1 cut(s) 978
SlaI CTCGAG 1 cut(s) 1841
SmiMI CAYNNNNRTG 2 cut(s) 99, 2283
SmlI CTYRAG 6 cut(s) 1102, 1150, 1841, 2059, 2395, 2536
SmoI CTYRAG 6 cut(s) 1102, 1150, 1841, 2059, 2395, 2536
SnaBI TACGTA 1 cut(s) 2115
SsiI CCGC 4 cut(s) 161, 467, 1434, 2146
SspDI GGCGCC 1 cut(s) 1923
SspI AATATT 2 cut(s) 1313, 2068
SspMI CTAG 6 cut(s) 900, 1076, 1614, 1832, 1895, 1991
SstI GAGCTC 1 cut(s) 2476
StyD4I CCNGG 2 cut(s) 684, 866
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 7 cut(s) 402, 463, 1090, 1955, 1971, 2037, 2198
TaiI ACGT 2 cut(s) 2117, 2414
TatI WGTACW 1 cut(s) 1717
TfiI GAWTC 7 cut(s) 367, 727, 971, 1209, 1400, 2137, 2318
TscAI CASTG 4 cut(s) 1741, 1794, 2285, 2357
TseFI GTSAC 2 cut(s) 661, 2641
TseI GCWGC 8 cut(s) 11, 14, 76, 761, 879, 1163, 1648, 1651
Tsp45I GTSAC 2 cut(s) 661, 2641
TspRI CASTG 4 cut(s) 1741, 1794, 2285, 2357
Tth111I GACNNNGTC 1 cut(s) 650
Van91I CCANNNNNTGG 1 cut(s) 1365
Vha464I CTTAAG 2 cut(s) 1150, 2395
VneI GTGCAC 1 cut(s) 1785
VpaK11BI GGWCC 1 cut(s) 978
XapI RAATTY 6 cut(s) 852, 1058, 1740, 2024, 2087, 2215
XceI RCATGY 3 cut(s) 88, 192, 1705
XhoI CTCGAG 1 cut(s) 1841
XmiI GTMKAC 3 cut(s) 1750, 2117, 2371
XspI CTAG 6 cut(s) 900, 1076, 1614, 1832, 1895, 1991
ZrmI AGTACT 1 cut(s) 1719
Zsp2I ATGCAT 3 cut(s) 418, 1141, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.