Rroxscaffold_7G00205720

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011520
Physical Location & Seq
Forward (+)
55357219 .. 55362152
4934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205720.1

Sequence Viewer

Length: 2988 bp
ATGGATCCTGGAACCGCAGCTACCATCTTGAGCTGTTTATGTCCCATAAACGTTTTACAAGAAACAACAGCGCAGCGTTGCAGCTATCTTAACAAGAATCCAAAAGAGAAGATAGAAGAGTTGAGATCAAAAATCGAAGAATTAAAGGCACTCTTTGAAGATGTGAATAGAAAACTAAAGCATGCATGTATTCAAGAAAGGAAAGCTCCCACAGCTCAAGTGAAACTGTGGTTGGAAAATGTTCAAAAGTTTAACAATAACGAGCGACGCGATCTACTTTCCCAGGAAGATAAGAGATGCTTATGTAGATATCTTCCAAATTATGCCTCTCGCAGAAAGTTGGGGAAACTTGTTGAAAAGACTATTAAGATGGTTGATGAGCTTCTTAGGAAGGGACAGTTAATTAAAGATGATTCATTGGCTTATTTATTGGTAGAGAACGTAAAGGCGCTTCCAACGACAGTAACAGTGAGCAATGAATCAACTAAAAGAACGATAGAAGAAATCTGGGGCCATCTGACAGCAGACCATGAAGTGAAAAAGATTGGTGTTTACGGCATCGGTGGCGTTGGAAAAACAACAGTTATGAAAGAAATAAATAACCGACTCAAAAGAGAGATGGAGGAAAAGAAGATCACTTGTTTTGACAATGTGATCTGGATCACAATGCCTAAGAGTTCAAATTCCGAAAAATTGCAGAAGACCATTGCAAAATCTGTGAGCTTGCATCTCCTTGATCATTGGGATACAATGGAAAACGCAGCAGCGTTACTGGAAGCCTTAACACGGAGGAAGAAGCTTTTGCTGATTTTTGATGATGTTTGGACTGGATTTTCTTTAGAAAATGTTGGAATTCCTGAACCAACCAGAGAAAATGGCTGCAAGTTCATATTAACTACTAGATCGTTAAGGGCGTGCCGAGCCATGGAGACAGATAAAGAAGTGGAGGTACAGATGCTTTCTGAGGATGAAACATGGAGCCTTTTCACTGAAAAAGTTGGTTCTACTGTTCTGCAGAAAACAGAAATTTATCTTCTTGCAAGGAAAGTAGCGAAAGAATTTGGTGGTCTTCCACTAGCCATTGTCACAGTCGGAAGAGCCTTGAGAAAAGTAGAGAACGAAATGGGGTGGCAGAATGCTTGGACTGCATTAAGAGAGTCTGCAGCTAATGTGGAAGGCATGGAAGAGATGGTGTTTTCATGTTTGAGATTCAGTTATGAAAGATTAGATGATGACATTATCCGATCATGTTTCCTATATTGTGCCTTGTATCCTGAAGGTCATCATATTGAAGCAAAAGAGTTAATTGAAAATTGGATGTGGGAGGGTTTGCTCGGTGATGTAGGAAGACTACAAGCTAATATATGGAAGGGACAAATGATTTTGGCTAATCTAAAGGATTCTTGTCTGCTAGATCATGTTAACGCAGAGAGTGGCATTGAATGTGTCAAAATGCACGACTTAATTAGGGACATGGCAATTGATATTACAAGAAAAAGTAGTCATGTTTGCCTCATCAGAGCTGGGATTGCAGTAGAAAGGATCCAAATTCAAAGTGAGGAGATTGAAGACGTGAAGAGGATATCTATGATGTATAATGATTTGATATGTCTTCCTAAGAATCTATCTTGTCCCAAGTTATCCTCTCTGCTGCTGCAGTATTGCTCCGCGGACAAAGTAACTGATCTTTGTTTTACCGGAATGCAGAATCTTAAAGTACTTGACTTGTCCTTCACTGGAATTTCCCGTCTGTCTGAGTCCATTGTCAACTTGAGGAATTTGCGTGCCTTGCTATTGCGTAGTTGTTGGAACCTAACAAGGCTGCCTTCTCTAGAATTGCTCGAGGCTCTGTGGGATTTGGATCTATCTTATACTCCCATCAGAAGCTTGCCATTAGGAATGGAAATGTTACGTCACCTTAAGCGCCTGAATCTTTCTTATACAGAATTGGACTCGTTTCCAGAAGGACTACTAACCACTCTCAATTTTCTAGAAGAATTGCTTTTGTTTGGTTCTCAATGTGCGTTTGGATTAGAACTTGTCAATGAACTCATCGCCTGCATAAACATCGTTGTTCTTGAAGTGCAATTCCAGGGCTCAGAAGTGTTTAATATTTATACAAGATCCGGGCATTGGAATAATCTACAGAGTTTCAGATTTGACATATGCTCATTCTTTTACCACAATATTGGAGCAATAAGGAAGCACGTTGAAAGGAGCAAGAGTATAACCTGCATAGGAAGTTTCTTTGTGAATGGTACTCGTCTTTTGCTCCCTGATTGCACTTCTGAACTGCAGCTCAGGCGCTGCAATGATGTTGATATATTGTCAAGATGCTTATCAGATTCCATCCAGTTGAAAAAATGTGCTGTCAGATACTGTAATCAAATGGAGAGTATAGTAACGACTGCGGAGAACAACTTGAGCAGTCTAAATTCGTTAAAGCTGTATTCTCTAAGAAATCTGAAGATGCTCTGCAAGGGTGTTGTCCCTCTGGGAACTTTCGGTGACCTTATGATATTGCAAATCAGTTGTTGTCATTCTATTGAGACTCTATTCACTGTAACGCTGATTGAACAGCTCGGAAACCTTGAAGAACTCACTATCAGATATTGTTTAAGATTGAAGGTGCTGATACAGGAAGAAGGGTCAATCCAGAGAGCTGAAGTCAGCCTTCCAAGACTTAAGAATCTGGAATTGTATACGTTGTTGGAACTGAAGAGCATTTATAGTGAAGACATAATTTGCAATTCTTTGAGCACAATTACTGTTCAGAAATGTCCAGGTCTAAAGAAGCTACCTATCACCATATCGACAGATCAAGGAGATCCACATCGACAACCATCAACCACTCCACCAATCAAAGAAATCATAGCAGAGAGATCGTGGTGGAATTCAGTAGAATTAGCTAATCGCGATGCAAAAACCTTCCTTCAGCCTAGACTGGTAGAGCCTACAATGATGTACTTGGAACTGGATGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_4 LRR_8 NB-ARC

Protein Analysis

995

Amino Acids

113.63

Weight (kDa)

6.42

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 163 - 335 5.4e-39 NB-ARC domain
WHD_DRP PF23559 423 - 493 1e-08 Disease resistance protein Winged helix domain
LRR_8 PF13855 545 - 602 2e-08 Leucine rich repeat
LRR_RPS2 PF23247 764 - 872 8.1e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 830 - 933 4.7e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2240
AccI GTMKAC 1 cut(s) 2702
AccII CGCG 3 cut(s) 270, 1670, 2916
AciI CCGC 4 cut(s) 15, 1668, 1670, 2411
AclI AACGTT 1 cut(s) 51
AclWI GGATC 7 cut(s) 12, 668, 1537, 1550, 1869, 2118, 2822
AcsI RAATTY 9 cut(s) 682, 852, 1026, 1058, 1548, 1740, 1777, 2434, 2893
AcuI CTGAAG 5 cut(s) 1296, 2486, 2685, 2738, 2918
AfaI GTAC 4 cut(s) 951, 1719, 2260, 2966
AfiI CCNNNNNNNGG 3 cut(s) 786, 925, 2133
AflII CTTAAG 2 cut(s) 1919, 2684
AjiI CACGTC 1 cut(s) 1573
AjnI CCWGG 4 cut(s) 7, 282, 2091, 2782
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
Alw21I GWGCWC 1 cut(s) 2762
Alw26I GTCTC 2 cut(s) 923, 2543
AlwI GGATC 7 cut(s) 12, 668, 1537, 1550, 1869, 2118, 2822
AlwNI CAGNNNCTG 2 cut(s) 2307, 2379
Ama87I CYCGRG 1 cut(s) 1841
AoxI GGCC 1 cut(s) 511
ApoI RAATTY 9 cut(s) 682, 852, 1026, 1058, 1548, 1740, 1777, 2434, 2893
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 4 cut(s) 73, 451, 1926, 2307
AspS9I GGNCC 1 cut(s) 511
AsuC2I CCSGG 1 cut(s) 2128
AsuHPI GGTGA 4 cut(s) 1349, 1907, 2519, 2797
AvaI CYCGRG 1 cut(s) 1841
BamHI GGATCC 2 cut(s) 4, 1542
BanII GRGCYC 1 cut(s) 2099
BarI GAAGNNNNNNTAC 4 cut(s) 435, 467, 933, 965
BbsI GAAGAC 6 cut(s) 707, 1061, 1354, 1575, 1604, 2742
Bbv12I GWGCWC 1 cut(s) 2762
BccI CCATC 8 cut(s) 32, 364, 522, 613, 1183, 1886, 2357, 2851
BceAI ACGGC 1 cut(s) 571
BcgI CGANNNNNNTGC 4 cut(s) 2050, 2084, 2865, 2899
BciT130I CCWGG 4 cut(s) 9, 284, 2093, 2784
BciVI GTATCC 2 cut(s) 739, 1281
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 1 cut(s) 2128
BcoDI GTCTC 2 cut(s) 923, 2543
BfaI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2940
BfmI CTRYAG 5 cut(s) 1013, 1161, 1655, 2144, 2294
BfoI RGCGCY 3 cut(s) 452, 1927, 2308
BfrI CTTAAG 2 cut(s) 1919, 2684
BfuAI ACCTGC 1 cut(s) 2240
BfuI GTATCC 2 cut(s) 739, 1281
BmcAI AGTACT 1 cut(s) 1719
Bme1390I CCNGG 5 cut(s) 9, 284, 2093, 2128, 2784
BmeT110I CYCGRG 1 cut(s) 1841
BmgBI CACGTC 1 cut(s) 1573
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 6 cut(s) 6, 13, 512, 980, 1544, 1811
BmrFI CCNGG 5 cut(s) 9, 284, 2093, 2128, 2784
BmsI GCATC 7 cut(s) 287, 567, 736, 945, 2324, 2460, 2908
BpiI GAAGAC 6 cut(s) 707, 1061, 1354, 1575, 1604, 2742
Bpu10I CCTNAGC 1 cut(s) 2300
BpuEI CTTGAG 5 cut(s) 49, 201, 1123, 1792, 2443
BpuMI CCSGG 1 cut(s) 2128
BsaBI GATNNNNATC 4 cut(s) 659, 1860, 2338, 2832
BsaJI CCNNGG 4 cut(s) 282, 924, 1668, 2092
BsaWI WCCGGW 1 cut(s) 1697
BsaXI ACNNNNNCTCC 2 cut(s) 2209, 2239
Bsc4I CCNNNNNNNGG 3 cut(s) 786, 925, 2133
Bse1I ACTGG 6 cut(s) 777, 832, 1741, 2353, 2949, 2979
Bse3DI GCAATG 3 cut(s) 481, 705, 2317
Bse8I GATNNNNATC 4 cut(s) 659, 1860, 2338, 2832
BseBI CCWGG 4 cut(s) 9, 284, 2093, 2784
BseDI CCNNGG 4 cut(s) 282, 924, 1668, 2092
BseGI GGATG 4 cut(s) 973, 1323, 2349, 2983
BseJI GATNNNNATC 4 cut(s) 659, 1860, 2338, 2832
BseLI CCNNNNNNNGG 3 cut(s) 786, 925, 2133
BseMI GCAATG 3 cut(s) 481, 705, 2317
BseMII CTCAG 4 cut(s) 954, 1746, 2112, 2314
BseNI ACTGG 6 cut(s) 777, 832, 1741, 2353, 2949, 2979
BseRI GAGGAG 1 cut(s) 1574
BseYI CCCAGC 1 cut(s) 1523
Bsh1236I CGCG 3 cut(s) 270, 1670, 2916
BshFI GGCC 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 2762
BsiHKCI CYCGRG 1 cut(s) 1841
BsiSI CCGG 2 cut(s) 1698, 2127
BslFI GGGAC 6 cut(s) 27, 408, 1386, 1484, 1617, 2474
BslI CCNNNNNNNGG 3 cut(s) 786, 925, 2133
BsmAI GTCTC 2 cut(s) 923, 2543
BsmFI GGGAC 6 cut(s) 27, 408, 1386, 1484, 1617, 2474
BsmI GAATGC 2 cut(s) 1141, 1707
BsnI GGCC 1 cut(s) 513
BsoBI CYCGRG 1 cut(s) 1841
Bsp1286I GDGCHC 2 cut(s) 2099, 2762
Bsp19I CCATGG 1 cut(s) 924
Bsp68I TCGCGA 1 cut(s) 2916
BspACI CCGC 4 cut(s) 15, 1668, 1670, 2411
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 4 cut(s) 955, 1747, 2111, 2313
BspFNI CGCG 3 cut(s) 270, 1670, 2916
BspLI GGNNCC 6 cut(s) 6, 13, 512, 980, 1544, 1811
BspMAI CTGCAG 4 cut(s) 1017, 1165, 1659, 2298
BspMI ACCTGC 1 cut(s) 2240
BspPI GGATC 7 cut(s) 12, 668, 1537, 1550, 1869, 2118, 2822
BspQI GCTCTTC 2 cut(s) 1090, 2714
BspTI CTTAAG 2 cut(s) 1919, 2684
BsrDI GCAATG 3 cut(s) 481, 705, 2317
BsrI ACTGG 6 cut(s) 777, 832, 1741, 2353, 2949, 2979
BssECI CCNNGG 4 cut(s) 282, 924, 1668, 2092
BssNAI GTATAC 1 cut(s) 2703
BssT1I CCWWGG 1 cut(s) 924
Bst1107I GTATAC 1 cut(s) 2703
Bst2UI CCWGG 4 cut(s) 9, 284, 2093, 2784
Bst6I CTCTTC 5 cut(s) 111, 1090, 1179, 1571, 2714
BstAFI CTTAAG 2 cut(s) 1919, 2684
BstC8I GCNNGC 6 cut(s) 183, 725, 916, 1785, 1889, 2059
BstDEI CTNAG 8 cut(s) 386, 672, 963, 1617, 1755, 2098, 2300, 2456
BstDSI CCRYGG 2 cut(s) 924, 1668
BstEII GGTNACC 1 cut(s) 2507
BstF5I GGATG 4 cut(s) 973, 1323, 2349, 2983
BstFNI CGCG 3 cut(s) 270, 1670, 2916
BstH2I RGCGCY 3 cut(s) 452, 1927, 2308
BstHHI GCGC 4 cut(s) 73, 451, 1926, 2307
BstMAI GTCTC 2 cut(s) 923, 2543
BstMWI GCNNNNNNNGC 7 cut(s) 212, 564, 920, 1145, 1529, 1789, 2302
BstNI CCWGG 4 cut(s) 9, 284, 2093, 2784
BstNSI RCATGY 2 cut(s) 185, 189
BstPI GGTNACC 1 cut(s) 2507
BstSCI CCNGG 5 cut(s) 7, 282, 2091, 2126, 2782
BstSFI CTRYAG 5 cut(s) 1013, 1161, 1655, 2144, 2294
BstUI CGCG 3 cut(s) 270, 1670, 2916
BstV2I GAAGAC 6 cut(s) 707, 1061, 1354, 1575, 1604, 2742
BstX2I RGATCY 5 cut(s) 4, 1542, 1861, 2123, 2827
BstXI CCANNNNNNTGG 1 cut(s) 2189
BstYI RGATCY 5 cut(s) 4, 1542, 1861, 2123, 2827
BstZ17I GTATAC 1 cut(s) 2703
BsuI GTATCC 2 cut(s) 739, 1281
BsuRI GGCC 1 cut(s) 513
BtgI CCRYGG 2 cut(s) 924, 1668
BtgZI GCGATG 2 cut(s) 2038, 2931
BtrI CACGTC 1 cut(s) 1573
BtsCI GGATG 4 cut(s) 973, 1323, 2349, 2983
BtsIMutI CAGTG 4 cut(s) 474, 987, 1734, 2559
BtuMI TCGCGA 1 cut(s) 2916
BveI ACCTGC 1 cut(s) 2240
Cac8I GCNNGC 6 cut(s) 183, 725, 916, 1785, 1889, 2059
CaiI CAGNNNCTG 2 cut(s) 2307, 2379
CfoI GCGC 4 cut(s) 73, 451, 1926, 2307
Cfr13I GGNCC 1 cut(s) 511
Cfr42I CCGCGG 1 cut(s) 1671
CseI GACGC 1 cut(s) 276
Csp6I GTAC 4 cut(s) 950, 1718, 2259, 2965
CviQI GTAC 4 cut(s) 950, 1718, 2259, 2965
DdeI CTNAG 8 cut(s) 386, 672, 963, 1617, 1755, 2098, 2300, 2456
Eam1104I CTCTTC 5 cut(s) 111, 1090, 1179, 1571, 2714
EarI CTCTTC 5 cut(s) 111, 1090, 1179, 1571, 2714
Eco130I CCWWGG 1 cut(s) 924
Eco24I GRGCYC 1 cut(s) 2099
Eco32I GATATC 2 cut(s) 311, 1584
Eco57I CTGAAG 5 cut(s) 1296, 2486, 2685, 2738, 2918
Eco88I CYCGRG 1 cut(s) 1841
Eco91I GGTNACC 1 cut(s) 2507
EcoO65I GGTNACC 1 cut(s) 2507
EcoRI GAATTC 2 cut(s) 852, 2893
EcoRII CCWGG 4 cut(s) 7, 282, 2091, 2782
EcoRV GATATC 2 cut(s) 311, 1584
EcoT14I CCWWGG 1 cut(s) 924
EcoT22I ATGCAT 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 2099
ErhI CCWWGG 1 cut(s) 924
FaqI GGGAC 6 cut(s) 27, 408, 1386, 1484, 1617, 2474
FauNDI CATATG 1 cut(s) 2165
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 1 cut(s) 2702
FokI GGATG 3 cut(s) 980, 1330, 2336
FriOI GRGCYC 1 cut(s) 2099
FspBI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2940
GlaI GCGC 4 cut(s) 72, 450, 1925, 2306
GsaI CCCAGC 1 cut(s) 1527
HaeII RGCGCY 3 cut(s) 452, 1927, 2308
HaeIII GGCC 1 cut(s) 513
HapII CCGG 2 cut(s) 1698, 2127
HgaI GACGC 1 cut(s) 276
HhaI GCGC 4 cut(s) 73, 451, 1926, 2307
Hin6I GCGC 4 cut(s) 71, 449, 1924, 2305
HinP1I GCGC 4 cut(s) 71, 449, 1924, 2305
HincII GTYRAC 2 cut(s) 1423, 1768
HindII GTYRAC 2 cut(s) 1423, 1768
HindIII AAGCTT 2 cut(s) 797, 1885
HpaI GTTAAC 1 cut(s) 1423
HpaII CCGG 2 cut(s) 1698, 2127
HphI GGTGA 4 cut(s) 1349, 1907, 2519, 2797
Hpy166II GTNNAC 4 cut(s) 553, 1423, 1768, 2703
Hpy8I GTNNAC 4 cut(s) 553, 1423, 1768, 2703
Hpy99I CGWCG 1 cut(s) 270
HpyCH4IV ACGT 6 cut(s) 51, 441, 1572, 1912, 2208, 2705
HpyF10VI GCNNNNNNNGC 7 cut(s) 212, 564, 920, 1145, 1529, 1789, 2302
HpyF3I CTNAG 8 cut(s) 386, 672, 963, 1617, 1755, 2098, 2300, 2456
HpySE526I ACGT 6 cut(s) 51, 441, 1572, 1912, 2208, 2705
HspAI GCGC 4 cut(s) 71, 449, 1924, 2305
Ksp22I TGATCA 1 cut(s) 736
KspAI GTTAAC 1 cut(s) 1423
KspI CCGCGG 1 cut(s) 1671
LguI GCTCTTC 2 cut(s) 1090, 2714
LmnI GCTCC 6 cut(s) 211, 978, 1670, 2192, 2217, 2277
LweI GCATC 7 cut(s) 287, 567, 736, 945, 2324, 2460, 2908
MaeI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2940
MaeII ACGT 6 cut(s) 51, 441, 1572, 1912, 2208, 2705
MaeIII GTNAC 9 cut(s) 463, 768, 1084, 1678, 1908, 1913, 2401, 2507, 2563
MfeI CAATTG 1 cut(s) 1479
MflI RGATCY 5 cut(s) 4, 1542, 1861, 2123, 2827
MhlI GDGCHC 2 cut(s) 2099, 2762
MlyI GAGTC 5 cut(s) 600, 1166, 1766, 1946, 2545
MmeI TCCRAC 7 cut(s) 213, 479, 550, 829, 1072, 1787, 2691
Mph1103I ATGCAT 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 1670
MspCI CTTAAG 2 cut(s) 1919, 2684
MspI CCGG 2 cut(s) 1698, 2127
MspR9I CCNGG 5 cut(s) 9, 284, 2093, 2128, 2784
MunI CAATTG 1 cut(s) 1479
Mva1269I GAATGC 2 cut(s) 1141, 1707
MvaI CCWGG 4 cut(s) 9, 284, 2093, 2784
MvnI CGCG 3 cut(s) 270, 1670, 2916
MwoI GCNNNNNNNGC 7 cut(s) 212, 564, 920, 1145, 1529, 1789, 2302
NciI CCSGG 1 cut(s) 2128
NcoI CCATGG 1 cut(s) 924
NdeI CATATG 1 cut(s) 2165
NlaIV GGNNCC 6 cut(s) 6, 13, 512, 980, 1544, 1811
NmeAIII GCCGAG 1 cut(s) 944
NmuCI GTSAC 3 cut(s) 1084, 1913, 2507
NruI TCGCGA 1 cut(s) 2916
NsiI ATGCAT 1 cut(s) 187
NspI RCATGY 2 cut(s) 185, 189
PacI TTAATTAA 1 cut(s) 405
PaeI GCATGC 1 cut(s) 185
PaeR7I CTCGAG 1 cut(s) 1841
PciSI GCTCTTC 2 cut(s) 1090, 2714
PcsI WCGNNNNNNNCGW 2 cut(s) 267, 911
PctI GAATGC 2 cut(s) 1141, 1707
PdmI GAANNNNTTC 1 cut(s) 240
PfoI TCCNGGA 1 cut(s) 7
PleI GAGTC 5 cut(s) 600, 1165, 1765, 1946, 2545
PpsI GAGTC 5 cut(s) 600, 1165, 1765, 1946, 2545
Psp1406I AACGTT 1 cut(s) 51
Psp6I CCWGG 4 cut(s) 7, 282, 2091, 2782
PspEI GGTNACC 1 cut(s) 2507
PspFI CCCAGC 1 cut(s) 1523
PspGI CCWGG 4 cut(s) 7, 282, 2091, 2782
PspN4I GGNNCC 6 cut(s) 6, 13, 512, 980, 1544, 1811
PspPI GGNCC 1 cut(s) 511
PspXI VCTCGAGB 1 cut(s) 1841
PsrI GAACNNNNNNTAC 1 cut(s) 36
PstI CTGCAG 4 cut(s) 1017, 1165, 1659, 2298
PstNI CAGNNNCTG 2 cut(s) 2307, 2379
PsuI RGATCY 5 cut(s) 4, 1542, 1861, 2123, 2827
RruI TCGCGA 1 cut(s) 2916
RsaI GTAC 4 cut(s) 951, 1719, 2260, 2966
RsaNI GTAC 4 cut(s) 950, 1718, 2259, 2965
SacII CCGCGG 1 cut(s) 1671
SapI GCTCTTC 2 cut(s) 1090, 2714
Sau96I GGNCC 1 cut(s) 511
ScaI AGTACT 1 cut(s) 1719
SchI GAGTC 5 cut(s) 600, 1166, 1766, 1946, 2545
ScrFI CCNGG 5 cut(s) 9, 284, 2093, 2128, 2784
SduI GDGCHC 2 cut(s) 2099, 2762
SfaNI GCATC 7 cut(s) 287, 567, 736, 945, 2324, 2460, 2908
SfcI CTRYAG 5 cut(s) 1013, 1161, 1655, 2144, 2294
Sfr274I CTCGAG 1 cut(s) 1841
Sfr303I CCGCGG 1 cut(s) 1671
SgrBI CCGCGG 1 cut(s) 1671
SlaI CTCGAG 1 cut(s) 1841
SmlI CTYRAG 8 cut(s) 28, 216, 1102, 1771, 1841, 1919, 2422, 2684
SmoI CTYRAG 8 cut(s) 28, 216, 1102, 1771, 1841, 1919, 2422, 2684
SphI GCATGC 1 cut(s) 185
SsiI CCGC 4 cut(s) 15, 1668, 1670, 2411
SspI AATATT 2 cut(s) 2113, 2188
SspMI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2940
StyD4I CCNGG 5 cut(s) 7, 282, 2091, 2126, 2782
StyI CCWWGG 1 cut(s) 924
TaiI ACGT 6 cut(s) 54, 444, 1575, 1915, 2211, 2708
TaqI TCGA 4 cut(s) 135, 1842, 2813, 2836
TatI WGTACW 2 cut(s) 1717, 2964
TscAI CASTG 4 cut(s) 474, 994, 1741, 2566
TseFI GTSAC 3 cut(s) 1084, 1913, 2507
Tsp45I GTSAC 3 cut(s) 1084, 1913, 2507
TspDTI ATGAA 9 cut(s) 405, 492, 546, 602, 877, 984, 1188, 1233, 2061
TspGWI ACGGA 1 cut(s) 802
TspRI CASTG 4 cut(s) 474, 994, 1741, 2566
Vha464I CTTAAG 2 cut(s) 1919, 2684
XapI RAATTY 9 cut(s) 682, 852, 1026, 1058, 1548, 1740, 1777, 2434, 2893
XbaI TCTAGA 2 cut(s) 1831, 1990
XceI RCATGY 2 cut(s) 185, 189
XhoI CTCGAG 1 cut(s) 1841
XmiI GTMKAC 1 cut(s) 2702
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2940
ZrmI AGTACT 1 cut(s) 1719
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.