Rroxscaffold_178G00437470

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000178
OG0011520
Physical Location & Seq
Forward (+)
204971 .. 207959
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_178G00437470.1

Sequence Viewer

Length: 2886 bp
ATGGATCCTGGAACCGCAGCTACCATCTTGAGCTGTTTATGTCCCATAAACGTTTTACAAGAAACAACAGCGCAGCGTTGCAGCTATCTTAACAAGAATCCAAAAGAGAAGATAGAAGAGTTGAGATCAAAAATCGAAGAATTAAAGGCACTCTTTGAAGATGTGAATAGAAAACTAAAGCATGCATGTATTCAAGAAAGGAAAGCTCCCACAGCTCAAGTGAAACTGTGGTTGGAAAATGTTCAAAAGTTTGACAATAACGAGCGACGCGATCTACTTTCCCAGGAAGAGAAGAGATGCTTATGTAGATATCTTCCAAATTATGCCTCTCGCAGAAAGTTGGGGAAACTTGTTGAAAAGACTATTAAGATGGTTGATGAGCTTCTTAGGAAGGGACAGTTAATTAAAGATGATTCATTGGCTTATTTATTGGTAGAGAACGTAAAGGCGCTTCCAACGACAGTAACAGTGAGCAATGAATCAACTAAAAGAACGATAGAAGAAATCTGGGGCCATCTGACAGCAGACCATGAAGTGAAAAAGATTGGTGTTTACGGCATCGGTGGCGTTGGAAAAACAACAGTTATGAAAGAAATAAATAACCGACTCAAAAGAGAGATGGAGGAAAAGAAGATCACTTGTTTTGACAATGTGATCTGGATCACAATGCCTAAGAGTTCAAATTCCGAAAAATTGCAGAAGACCATTGCAAAATCTGTGAGCTTGCATCTCCTTGATCATTGGGATACAATGGAAAACGCAGCAGCGTTACTGGAAGCCTTAACACGGAGGAAGAAGCTTTTGCTGATTTTTGATGATGTTTGGACTGGATTTTCTTTAGAAAATGTTGGAATTCCTGAACCAACCAGAGAAAATGGCTGCAAGTTCATATTAACTACTAGATCGTTAAGGGCGTGCCGAGCCATGGAGACAGATAAAGAAGTGGAGGTACAGATGCTTTCTGAGGATGAAACATGGAGCCTTTTCACTGAAAAAGTTGGTTCTACTGTTCTGCAGAAAACAGAAATTTATCTTCTTGCAAGGAAAGTAGCGAAAGAATTTGGTGGTCTTCCACTAGCCATTGTCACAGTCGGAAGAGCCTTGAGAAAAGTAGAGAACGAAATGGGGTGGCAGAATGCTTTGACTGCATTAAGAGAGTCTGCAGCTAATGTGGAAGGCATGGAAGAGATGGTGTTTTCATGTTTGAGATTCAGTTATGAAAGATTAGATGATGACATTATCCGATCATGTTTCCTATATTGTGCCTTGTATCCTGAAGGTCATCATATTGAAGCAAAAGAGTTAATTGAAAATTGGATGTGGGAGGGTTTGCTCGGTGATGTAGGAAGACTACAAGCTAATATATGGAAGGGACAAATGATTTTGGCTAATCTAAAGGATTCTTGTCTGCTAGATCATGTTAACGCAGAGAGTGGCATTGAATGTGTCAAAATGCACGACTTAATTAGGGACATGGCAATTGATATTACAAGAAAAAGTAGTCATGTTTGCCTCATCAGAGCTGGGATTGCAGTAGAAAGGATCCAAATTCAAAGTGAGGAGATTGAAGACGTGAAGAGGATATCTATGATGTATAATGATTTGATATGTCTTCCTAAGAATCTATCTTGTCCCAAGTTATCCTCTCTGCTGCTGCAGTATTGCTCCGCGGACAAAGTAACTGATCTTTGTTTTACCGGAATGCAGAATCTTAAAGTACTTGACTTGTCCTTCACTGGAATTTCCCGTCTGTCTGAGTCCATTGTCAACTTGAGGAATTTGCGTGCCTTGCTATTGCGTAGTTGTTGGAACCTAACAAGGCTGCCTTCTCTAGAATTGCTCGAGGCTCTGTGGGATTTGGATCTATCTTATACTCCCATCAGAAGCTTGCCATTAGGAATGGAAATGTTACGTCACCTTAAGCGCCTGAATCTTTCTTATACAGAATTGGACTCGTTTCCAGAAGGACTACTAACCACTCTCAATTTTCTAGAAGAATTGCTTTTTGTTTGGTTCTCAATATCCGGGCATTGGAATAATCTACAGAGTTTCAGATTTGACATATGCTCATTCTTTTACCACAATATTGGAGCAATAAGGAAGCACGTTGAAAGGAGCAAGAGTATAACCTGCATAGGAAGTTTCTTTGTGAATGGTACTCGTCTTTTGCTCCCTGATTGCACTTCTGAACTGCAGCTCAGGCGCTGCAATGATGTTGATATATTGTCAAGATGCTTATCAGATTCCATCCAGTTGAAAAAATGTGCTGTCAGATACTGTAATCAAATGGAGAGTATAGTAACGACTGCGGAGAACAACTTGAGCAGTCTAAATTCGTTAAAGCTGTATTCTCTAAGAAATCTGAAGATGCTCTGCAAGGGTGTTGTCCCTCTGGGAACTTTCGGTGACCTTATGATATTGCAAATCAGTTGTTGTCATTCTATTGAGACTCTGTTCACTGTAACGCTGATTGAACAGCTCGGAAACCTTGAAGAACTCACTATCAGATATTGTTTAAGATTGAAGGTGCTGATACAGGAAGAAGGGTCAATCCAGAGAGCTGAAGTCAGCCTTCCAAGACTTAAGAATCTGGAATTGTATACGTTGTTGGAACTGAAGAGCATTTATAGTGAAGACATAATTTGCAATTCTTTGAGCACAATTACTGTTCAGAAATGTCCAGGTCTAAAGAAGCTACCTATCACCATATCGACAGATCAAGGAGATCCACATCGACAACCATCAACCACTCCACCAATCAAAGAAATCATAGCAGAGAGATCGTGGTGGAATTCAGTAGAATTAGCTAATCGCGATGCAAAAACCTTCCTTCAGCCTAGACTGGTAGAGCCTACAATGATGTACTTGGAACTGGATGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_4 LRR_8 NB-ARC

Protein Analysis

961

Amino Acids

109.89

Weight (kDa)

6.64

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 163 - 335 5.1e-39 NB-ARC domain
WHD_DRP PF23559 423 - 493 9.7e-09 Disease resistance protein Winged helix domain
LRR_8 PF13855 545 - 602 1.9e-08 Leucine rich repeat
LRR_RPS2 PF23247 730 - 838 7.2e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 788 - 899 1.6e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2138
AccI GTMKAC 1 cut(s) 2600
AccII CGCG 3 cut(s) 270, 1670, 2814
AciI CCGC 4 cut(s) 15, 1668, 1670, 2309
AclI AACGTT 1 cut(s) 51
AclWI GGATC 6 cut(s) 12, 668, 1537, 1550, 1869, 2720
AcsI RAATTY 9 cut(s) 682, 852, 1026, 1058, 1548, 1740, 1777, 2332, 2791
AcuI CTGAAG 5 cut(s) 1296, 2384, 2583, 2636, 2816
AfaI GTAC 4 cut(s) 951, 1719, 2158, 2864
AfiI CCNNNNNNNGG 3 cut(s) 786, 925, 2031
AflII CTTAAG 2 cut(s) 1919, 2582
AjiI CACGTC 1 cut(s) 1573
AjnI CCWGG 3 cut(s) 7, 282, 2680
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
Alw21I GWGCWC 1 cut(s) 2660
Alw26I GTCTC 2 cut(s) 923, 2441
AlwI GGATC 6 cut(s) 12, 668, 1537, 1550, 1869, 2720
AlwNI CAGNNNCTG 2 cut(s) 2205, 2277
Ama87I CYCGRG 1 cut(s) 1841
AoxI GGCC 1 cut(s) 511
ApoI RAATTY 9 cut(s) 682, 852, 1026, 1058, 1548, 1740, 1777, 2332, 2791
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 4 cut(s) 73, 451, 1926, 2205
AspS9I GGNCC 1 cut(s) 511
AsuC2I CCSGG 1 cut(s) 2026
AsuHPI GGTGA 4 cut(s) 1349, 1907, 2417, 2695
AvaI CYCGRG 1 cut(s) 1841
BamHI GGATCC 2 cut(s) 4, 1542
BarI GAAGNNNNNNTAC 4 cut(s) 435, 467, 933, 965
BbsI GAAGAC 6 cut(s) 707, 1061, 1354, 1575, 1604, 2640
Bbv12I GWGCWC 1 cut(s) 2660
BccI CCATC 8 cut(s) 32, 364, 522, 613, 1183, 1886, 2255, 2749
BceAI ACGGC 1 cut(s) 571
BcgI CGANNNNNNTGC 2 cut(s) 2763, 2797
BciT130I CCWGG 3 cut(s) 9, 284, 2682
BciVI GTATCC 2 cut(s) 739, 1281
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 1 cut(s) 2026
BcoDI GTCTC 2 cut(s) 923, 2441
BfaI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2838
BfmI CTRYAG 5 cut(s) 1013, 1161, 1655, 2042, 2192
BfoI RGCGCY 3 cut(s) 452, 1927, 2206
BfrI CTTAAG 2 cut(s) 1919, 2582
BfuAI ACCTGC 1 cut(s) 2138
BfuI GTATCC 2 cut(s) 739, 1281
BmcAI AGTACT 1 cut(s) 1719
Bme1390I CCNGG 4 cut(s) 9, 284, 2026, 2682
BmeT110I CYCGRG 1 cut(s) 1841
BmgBI CACGTC 1 cut(s) 1573
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 6 cut(s) 6, 13, 512, 980, 1544, 1811
BmrFI CCNGG 4 cut(s) 9, 284, 2026, 2682
BmsI GCATC 7 cut(s) 287, 567, 736, 945, 2222, 2358, 2806
BpiI GAAGAC 6 cut(s) 707, 1061, 1354, 1575, 1604, 2640
Bpu10I CCTNAGC 1 cut(s) 2198
BpuEI CTTGAG 5 cut(s) 49, 201, 1123, 1792, 2341
BpuMI CCSGG 1 cut(s) 2026
BsaBI GATNNNNATC 4 cut(s) 659, 1860, 2236, 2730
BsaJI CCNNGG 3 cut(s) 282, 924, 1668
BsaWI WCCGGW 1 cut(s) 1697
BsaXI ACNNNNNCTCC 2 cut(s) 2107, 2137
Bsc4I CCNNNNNNNGG 3 cut(s) 786, 925, 2031
Bse1I ACTGG 6 cut(s) 777, 832, 1741, 2251, 2847, 2877
Bse3DI GCAATG 3 cut(s) 481, 705, 2215
Bse8I GATNNNNATC 4 cut(s) 659, 1860, 2236, 2730
BseBI CCWGG 3 cut(s) 9, 284, 2682
BseDI CCNNGG 3 cut(s) 282, 924, 1668
BseGI GGATG 4 cut(s) 973, 1323, 2247, 2881
BseJI GATNNNNATC 4 cut(s) 659, 1860, 2236, 2730
BseLI CCNNNNNNNGG 3 cut(s) 786, 925, 2031
BseMI GCAATG 3 cut(s) 481, 705, 2215
BseMII CTCAG 3 cut(s) 954, 1746, 2212
BseNI ACTGG 6 cut(s) 777, 832, 1741, 2251, 2847, 2877
BseRI GAGGAG 1 cut(s) 1574
BseYI CCCAGC 1 cut(s) 1523
Bsh1236I CGCG 3 cut(s) 270, 1670, 2814
BshFI GGCC 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 2660
BsiHKCI CYCGRG 1 cut(s) 1841
BsiSI CCGG 2 cut(s) 1698, 2025
BslFI GGGAC 6 cut(s) 27, 408, 1386, 1484, 1617, 2372
BslI CCNNNNNNNGG 3 cut(s) 786, 925, 2031
BsmAI GTCTC 2 cut(s) 923, 2441
BsmFI GGGAC 6 cut(s) 27, 408, 1386, 1484, 1617, 2372
BsmI GAATGC 2 cut(s) 1141, 1707
BsnI GGCC 1 cut(s) 513
BsoBI CYCGRG 1 cut(s) 1841
Bsp1286I GDGCHC 1 cut(s) 2660
Bsp19I CCATGG 1 cut(s) 924
Bsp68I TCGCGA 1 cut(s) 2814
BspACI CCGC 4 cut(s) 15, 1668, 1670, 2309
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 3 cut(s) 955, 1747, 2211
BspFNI CGCG 3 cut(s) 270, 1670, 2814
BspLI GGNNCC 6 cut(s) 6, 13, 512, 980, 1544, 1811
BspMAI CTGCAG 4 cut(s) 1017, 1165, 1659, 2196
BspMI ACCTGC 1 cut(s) 2138
BspPI GGATC 6 cut(s) 12, 668, 1537, 1550, 1869, 2720
BspQI GCTCTTC 2 cut(s) 1090, 2612
BspTI CTTAAG 2 cut(s) 1919, 2582
BsrDI GCAATG 3 cut(s) 481, 705, 2215
BsrI ACTGG 6 cut(s) 777, 832, 1741, 2251, 2847, 2877
BssECI CCNNGG 3 cut(s) 282, 924, 1668
BssNAI GTATAC 1 cut(s) 2601
BssT1I CCWWGG 1 cut(s) 924
Bst1107I GTATAC 1 cut(s) 2601
Bst2UI CCWGG 3 cut(s) 9, 284, 2682
Bst6I CTCTTC 7 cut(s) 111, 282, 287, 1090, 1179, 1571, 2612
BstAFI CTTAAG 2 cut(s) 1919, 2582
BstC8I GCNNGC 5 cut(s) 183, 725, 916, 1785, 1889
BstDEI CTNAG 7 cut(s) 386, 672, 963, 1617, 1755, 2198, 2354
BstDSI CCRYGG 2 cut(s) 924, 1668
BstEII GGTNACC 1 cut(s) 2405
BstF5I GGATG 4 cut(s) 973, 1323, 2247, 2881
BstFNI CGCG 3 cut(s) 270, 1670, 2814
BstH2I RGCGCY 3 cut(s) 452, 1927, 2206
BstHHI GCGC 4 cut(s) 73, 451, 1926, 2205
BstMAI GTCTC 2 cut(s) 923, 2441
BstMWI GCNNNNNNNGC 7 cut(s) 212, 564, 920, 1145, 1529, 1789, 2200
BstNI CCWGG 3 cut(s) 9, 284, 2682
BstNSI RCATGY 2 cut(s) 185, 189
BstPI GGTNACC 1 cut(s) 2405
BstSCI CCNGG 4 cut(s) 7, 282, 2024, 2680
BstSFI CTRYAG 5 cut(s) 1013, 1161, 1655, 2042, 2192
BstUI CGCG 3 cut(s) 270, 1670, 2814
BstV2I GAAGAC 6 cut(s) 707, 1061, 1354, 1575, 1604, 2640
BstX2I RGATCY 4 cut(s) 4, 1542, 1861, 2725
BstXI CCANNNNNNTGG 1 cut(s) 2087
BstYI RGATCY 4 cut(s) 4, 1542, 1861, 2725
BstZ17I GTATAC 1 cut(s) 2601
BsuI GTATCC 2 cut(s) 739, 1281
BsuRI GGCC 1 cut(s) 513
BtgI CCRYGG 2 cut(s) 924, 1668
BtgZI GCGATG 1 cut(s) 2829
BtrI CACGTC 1 cut(s) 1573
BtsCI GGATG 4 cut(s) 973, 1323, 2247, 2881
BtsIMutI CAGTG 4 cut(s) 474, 987, 1734, 2457
BtuMI TCGCGA 1 cut(s) 2814
BveI ACCTGC 1 cut(s) 2138
Cac8I GCNNGC 5 cut(s) 183, 725, 916, 1785, 1889
CaiI CAGNNNCTG 2 cut(s) 2205, 2277
CfoI GCGC 4 cut(s) 73, 451, 1926, 2205
Cfr13I GGNCC 1 cut(s) 511
Cfr42I CCGCGG 1 cut(s) 1671
CseI GACGC 1 cut(s) 276
Csp6I GTAC 4 cut(s) 950, 1718, 2157, 2863
CviQI GTAC 4 cut(s) 950, 1718, 2157, 2863
DdeI CTNAG 7 cut(s) 386, 672, 963, 1617, 1755, 2198, 2354
Eam1104I CTCTTC 7 cut(s) 111, 282, 287, 1090, 1179, 1571, 2612
EarI CTCTTC 7 cut(s) 111, 282, 287, 1090, 1179, 1571, 2612
Eco130I CCWWGG 1 cut(s) 924
Eco32I GATATC 2 cut(s) 311, 1584
Eco57I CTGAAG 5 cut(s) 1296, 2384, 2583, 2636, 2816
Eco88I CYCGRG 1 cut(s) 1841
Eco91I GGTNACC 1 cut(s) 2405
EcoO65I GGTNACC 1 cut(s) 2405
EcoRI GAATTC 2 cut(s) 852, 2791
EcoRII CCWGG 3 cut(s) 7, 282, 2680
EcoRV GATATC 2 cut(s) 311, 1584
EcoT14I CCWWGG 1 cut(s) 924
EcoT22I ATGCAT 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 924
FaqI GGGAC 6 cut(s) 27, 408, 1386, 1484, 1617, 2372
FauNDI CATATG 1 cut(s) 2063
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 1 cut(s) 2600
FokI GGATG 3 cut(s) 980, 1330, 2234
FspBI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2838
GlaI GCGC 4 cut(s) 72, 450, 1925, 2204
GsaI CCCAGC 1 cut(s) 1527
HaeII RGCGCY 3 cut(s) 452, 1927, 2206
HaeIII GGCC 1 cut(s) 513
HapII CCGG 2 cut(s) 1698, 2025
HgaI GACGC 1 cut(s) 276
HhaI GCGC 4 cut(s) 73, 451, 1926, 2205
Hin6I GCGC 4 cut(s) 71, 449, 1924, 2203
HinP1I GCGC 4 cut(s) 71, 449, 1924, 2203
HincII GTYRAC 2 cut(s) 1423, 1768
HindII GTYRAC 2 cut(s) 1423, 1768
HindIII AAGCTT 2 cut(s) 797, 1885
HpaI GTTAAC 1 cut(s) 1423
HpaII CCGG 2 cut(s) 1698, 2025
HphI GGTGA 4 cut(s) 1349, 1907, 2417, 2695
Hpy166II GTNNAC 5 cut(s) 553, 1423, 1768, 2457, 2601
Hpy8I GTNNAC 5 cut(s) 553, 1423, 1768, 2457, 2601
Hpy99I CGWCG 1 cut(s) 270
HpyCH4IV ACGT 6 cut(s) 51, 441, 1572, 1912, 2106, 2603
HpyF10VI GCNNNNNNNGC 7 cut(s) 212, 564, 920, 1145, 1529, 1789, 2200
HpyF3I CTNAG 7 cut(s) 386, 672, 963, 1617, 1755, 2198, 2354
HpySE526I ACGT 6 cut(s) 51, 441, 1572, 1912, 2106, 2603
HspAI GCGC 4 cut(s) 71, 449, 1924, 2203
Ksp22I TGATCA 1 cut(s) 736
KspAI GTTAAC 1 cut(s) 1423
KspI CCGCGG 1 cut(s) 1671
LguI GCTCTTC 2 cut(s) 1090, 2612
LmnI GCTCC 6 cut(s) 211, 978, 1670, 2090, 2115, 2175
LweI GCATC 7 cut(s) 287, 567, 736, 945, 2222, 2358, 2806
MaeI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2838
MaeII ACGT 6 cut(s) 51, 441, 1572, 1912, 2106, 2603
MaeIII GTNAC 9 cut(s) 463, 768, 1084, 1678, 1908, 1913, 2299, 2405, 2461
MfeI CAATTG 1 cut(s) 1479
MflI RGATCY 4 cut(s) 4, 1542, 1861, 2725
MhlI GDGCHC 1 cut(s) 2660
MlyI GAGTC 5 cut(s) 600, 1166, 1766, 1946, 2443
MmeI TCCRAC 7 cut(s) 213, 479, 550, 829, 1072, 1787, 2589
Mph1103I ATGCAT 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 1670
MspCI CTTAAG 2 cut(s) 1919, 2582
MspI CCGG 2 cut(s) 1698, 2025
MspR9I CCNGG 4 cut(s) 9, 284, 2026, 2682
MunI CAATTG 1 cut(s) 1479
Mva1269I GAATGC 2 cut(s) 1141, 1707
MvaI CCWGG 3 cut(s) 9, 284, 2682
MvnI CGCG 3 cut(s) 270, 1670, 2814
MwoI GCNNNNNNNGC 7 cut(s) 212, 564, 920, 1145, 1529, 1789, 2200
NciI CCSGG 1 cut(s) 2026
NcoI CCATGG 1 cut(s) 924
NdeI CATATG 1 cut(s) 2063
NlaIV GGNNCC 6 cut(s) 6, 13, 512, 980, 1544, 1811
NmeAIII GCCGAG 1 cut(s) 944
NmuCI GTSAC 3 cut(s) 1084, 1913, 2405
NruI TCGCGA 1 cut(s) 2814
NsiI ATGCAT 1 cut(s) 187
NspI RCATGY 2 cut(s) 185, 189
PacI TTAATTAA 1 cut(s) 405
PaeI GCATGC 1 cut(s) 185
PaeR7I CTCGAG 1 cut(s) 1841
PciSI GCTCTTC 2 cut(s) 1090, 2612
PcsI WCGNNNNNNNCGW 2 cut(s) 267, 911
PctI GAATGC 2 cut(s) 1141, 1707
PdmI GAANNNNTTC 1 cut(s) 240
PfoI TCCNGGA 1 cut(s) 7
PleI GAGTC 5 cut(s) 600, 1165, 1765, 1946, 2443
PpsI GAGTC 5 cut(s) 600, 1165, 1765, 1946, 2443
Psp1406I AACGTT 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 7, 282, 2680
PspEI GGTNACC 1 cut(s) 2405
PspFI CCCAGC 1 cut(s) 1523
PspGI CCWGG 3 cut(s) 7, 282, 2680
PspN4I GGNNCC 6 cut(s) 6, 13, 512, 980, 1544, 1811
PspPI GGNCC 1 cut(s) 511
PspXI VCTCGAGB 1 cut(s) 1841
PsrI GAACNNNNNNTAC 1 cut(s) 36
PstI CTGCAG 4 cut(s) 1017, 1165, 1659, 2196
PstNI CAGNNNCTG 2 cut(s) 2205, 2277
PsuI RGATCY 4 cut(s) 4, 1542, 1861, 2725
RruI TCGCGA 1 cut(s) 2814
RsaI GTAC 4 cut(s) 951, 1719, 2158, 2864
RsaNI GTAC 4 cut(s) 950, 1718, 2157, 2863
SacII CCGCGG 1 cut(s) 1671
SapI GCTCTTC 2 cut(s) 1090, 2612
Sau96I GGNCC 1 cut(s) 511
ScaI AGTACT 1 cut(s) 1719
SchI GAGTC 5 cut(s) 600, 1166, 1766, 1946, 2443
ScrFI CCNGG 4 cut(s) 9, 284, 2026, 2682
SduI GDGCHC 1 cut(s) 2660
SfaNI GCATC 7 cut(s) 287, 567, 736, 945, 2222, 2358, 2806
SfcI CTRYAG 5 cut(s) 1013, 1161, 1655, 2042, 2192
Sfr274I CTCGAG 1 cut(s) 1841
Sfr303I CCGCGG 1 cut(s) 1671
SgrBI CCGCGG 1 cut(s) 1671
SlaI CTCGAG 1 cut(s) 1841
SmlI CTYRAG 8 cut(s) 28, 216, 1102, 1771, 1841, 1919, 2320, 2582
SmoI CTYRAG 8 cut(s) 28, 216, 1102, 1771, 1841, 1919, 2320, 2582
SphI GCATGC 1 cut(s) 185
SsiI CCGC 4 cut(s) 15, 1668, 1670, 2309
SspI AATATT 1 cut(s) 2086
SspMI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2838
StyD4I CCNGG 4 cut(s) 7, 282, 2024, 2680
StyI CCWWGG 1 cut(s) 924
TaiI ACGT 6 cut(s) 54, 444, 1575, 1915, 2109, 2606
TaqI TCGA 4 cut(s) 135, 1842, 2711, 2734
TatI WGTACW 2 cut(s) 1717, 2862
TscAI CASTG 4 cut(s) 474, 994, 1741, 2464
TseFI GTSAC 3 cut(s) 1084, 1913, 2405
Tsp45I GTSAC 3 cut(s) 1084, 1913, 2405
TspDTI ATGAA 8 cut(s) 405, 492, 546, 602, 877, 984, 1188, 1233
TspGWI ACGGA 1 cut(s) 802
TspRI CASTG 4 cut(s) 474, 994, 1741, 2464
Vha464I CTTAAG 2 cut(s) 1919, 2582
XapI RAATTY 9 cut(s) 682, 852, 1026, 1058, 1548, 1740, 1777, 2332, 2791
XbaI TCTAGA 2 cut(s) 1831, 1990
XceI RCATGY 2 cut(s) 185, 189
XhoI CTCGAG 1 cut(s) 1841
XmiI GTMKAC 1 cut(s) 2600
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 6 cut(s) 900, 1076, 1412, 1832, 1991, 2838
ZrmI AGTACT 1 cut(s) 1719
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.