FvH4_2g07021

N-acetyl-gamma-glutamyl-phosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007780
Physical Location & Seq
Reverse (-)
5782665 .. 5788803
6139 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07021.t3

Sequence Viewer

Length: 1182 bp
ATGAGCTCTGCAACTCTCAGCTCCAATTTTCTCCAAACTGGGTGCCAATGGAAGGGTGGAGTGAAGAATTTAGAGGGTAATAAGCGAAATGCAGGGAAGGTGTTTGTGCAATGTTCTGTGAATTCAAAAACCCAGAAAGCAGAGAAGCAAGTTCGCCTTGGTGTTCTTGGGGCCAGTGGTTATACTGGTTCTGAGATTGTTCGGCTGCTGGCAAATCATCCTCATTTTGGCATTACCTTAATGACTGCTGATAGAAAAGCTGGACAGTCCATTGGATCAGTGTTTCCTCATCTAATTTGTCAAGATCTGCCAAAGATGGTTGCTATCAAGGATGCTGATTTTTCTGATGTGGATGCAGTATTTTGTTGTTTACCACATGGAACAACACAGGAAATAATCAAGGGCCTTCCAAAGAGTTTAAAGATTGTTGATCTGTCTGCTGACTTCCGTCTACAAGATATATCTGAGTATGAAGAATGGTACGGTCAGGCACATAGAGCACCAGATTTGCAGAAAGAAGCTGTGTATGGTTTGACAGAGATTTTGAGGGAGGAGATTAAAAGTGCACGTCTAGTTGCTAACCCTGGCTGTTATCCGACGTCTATTCAGCTTCCCCTTGTTCCACTATTTAAGGCTAATCTCATTCAACATAAAAACATTATTATAGACGCAAAATCTGGTGTTAGTGGAGCAGGACGTGGTGCCAAGGAGGCAAATTTGTACACTGAAATAGCTGAAGGCTTATATTCTTATGGCATTACCCGTCATCGCCATGTACCGGAAATTGAGCAGGGACTATCTGGTGCTACCAATTCAAAAGTAACCATTAGTTTTACTCCGCATCTAATGCCAATGAGCCGTGGTATGCAATCAACTATTTATGTGGAAATGGCTCCAGGGGTTGAAATCAAGGACTTGCAGCAACAGTTGAGGTTATCCTATGAGGATGAAGAATTTGTAGTTTTGTTGGAGGACGGTGTTGTTCCTCGCACGCATAATACTCGAGGGTCTAATTATTGTTTCATCAATGTCTTTCCTGATCGAATCCCAGGAAGAGCAATAATAATTTCAGTTATCGATAATCTTGTCAAGGGAGCTTCAGGTCAGGCTCTACAGAATCTTAATGTGATGATGGGATTTCCCGAGAGCACAGGGCTTCTATATCAGCCATTGTTTCCTTAA

Protein Analysis

394

Amino Acids

43.09

Weight (kDa)

7.13

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Semialdhyde_dh PF01118 53 - 189 6.1e-28 Semialdehyde dehydrogenase, NAD binding domain
Semialdhyde_dhC_1 PF22698 197 - 361 2.3e-57 Semialdehyde dehydrogenase, dimerisation domain
Semialdhyde_dhC PF02774 206 - 364 1.6e-19 Semialdehyde dehydrogenase, dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 602
AccB1I GGYRCC 2 cut(s) 42, 701
AccI GTMKAC 1 cut(s) 451
AciI CCGC 1 cut(s) 839
AclWI GGATC 1 cut(s) 283
AcsI RAATTY 4 cut(s) 67, 121, 715, 953
AcuI CTGAAG 2 cut(s) 756, 1083
AcyI GRCGYC 1 cut(s) 599
AfaI GTAC 3 cut(s) 482, 722, 777
AfiI CCNNNNNNNGG 3 cut(s) 52, 227, 778
AgsI TTSAA 4 cut(s) 126, 647, 816, 905
AjiI CACGTC 2 cut(s) 569, 698
AjnI CCWGG 3 cut(s) 583, 895, 1048
AluBI AGCT 7 cut(s) 6, 21, 260, 521, 610, 734, 1097
AluI AGCT 7 cut(s) 6, 21, 260, 521, 610, 734, 1097
Alw21I GWGCWC 4 cut(s) 8, 502, 568, 1151
Alw44I GTGCAC 1 cut(s) 564
AlwI GGATC 1 cut(s) 283
Ama87I CYCGRG 2 cut(s) 1002, 1142
AoxI GGCC 2 cut(s) 171, 403
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 2 cut(s) 205, 919
ApoI RAATTY 4 cut(s) 67, 121, 715, 953
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
AspS9I GGNCC 2 cut(s) 171, 403
AvaI CYCGRG 2 cut(s) 1002, 1142
BaeGI GKGCMC 1 cut(s) 568
BanI GGYRCC 2 cut(s) 42, 701
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 4 cut(s) 8, 502, 568, 1151
BbvI GCAGC 2 cut(s) 192, 931
BccI CCATC 2 cut(s) 310, 1126
BceAI ACGGC 1 cut(s) 843
BcgI CGANNNNNNTGC 2 cut(s) 983, 1017
BciT130I CCWGG 3 cut(s) 585, 897, 1050
BfaI CTAG 1 cut(s) 572
BfmI CTRYAG 1 cut(s) 1112
BglI GCCNNNNNGGC 1 cut(s) 710
BglII AGATCT 1 cut(s) 304
BisI GCNGC 2 cut(s) 206, 920
BlsI GCNGC 2 cut(s) 207, 921
Bme1390I CCNGG 3 cut(s) 585, 897, 1050
BmeT110I CYCGRG 2 cut(s) 1002, 1142
BmgBI CACGTC 2 cut(s) 569, 698
BmgT120I GGNCC 2 cut(s) 171, 403
BmiI GGNNCC 4 cut(s) 44, 172, 703, 894
BmrFI CCNGG 3 cut(s) 585, 897, 1050
BmrI ACTGGG 1 cut(s) 48
BmsI GCATC 3 cut(s) 322, 343, 850
BmuI ACTGGG 1 cut(s) 48
BoxI GACNNNNGTC 1 cut(s) 447
BpmI CTGGAG 1 cut(s) 879
Bsa29I ATCGAT 1 cut(s) 1077
BsaHI GRCGYC 1 cut(s) 599
BsaJI CCNNGG 6 cut(s) 157, 583, 705, 859, 896, 1048
BsaWI WCCGGW 1 cut(s) 778
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 227, 778
Bse1I ACTGG 3 cut(s) 43, 174, 190
Bse3DI GCAATG 1 cut(s) 116
BseBI CCWGG 3 cut(s) 585, 897, 1050
BseCI ATCGAT 1 cut(s) 1077
BseDI CCNNGG 6 cut(s) 157, 583, 705, 859, 896, 1048
BseGI GGATG 4 cut(s) 217, 337, 358, 952
BseLI CCNNNNNNNGG 3 cut(s) 52, 227, 778
BseMI GCAATG 1 cut(s) 116
BseMII CTCAG 3 cut(s) 31, 183, 456
BseNI ACTGG 3 cut(s) 43, 174, 190
BseRI GAGGAG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 2 cut(s) 192, 931
BshFI GGCC 2 cut(s) 173, 405
BshNI GGYRCC 2 cut(s) 42, 701
BshVI ATCGAT 1 cut(s) 1077
BsiHKAI GWGCWC 4 cut(s) 8, 502, 568, 1151
BsiHKCI CYCGRG 2 cut(s) 1002, 1142
BsiSI CCGG 1 cut(s) 779
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 3 cut(s) 52, 227, 778
BsmFI GGGAC 1 cut(s) 807
BsnI GGCC 2 cut(s) 173, 405
BsoBI CYCGRG 2 cut(s) 1002, 1142
Bsp1286I GDGCHC 4 cut(s) 8, 502, 568, 1151
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 4 cut(s) 275, 304, 430, 1039
BspACI CCGC 1 cut(s) 839
BspANI GGCC 2 cut(s) 173, 405
BspCNI CTCAG 3 cut(s) 30, 184, 457
BspDI ATCGAT 1 cut(s) 1077
BspLI GGNNCC 4 cut(s) 44, 172, 703, 894
BspPI GGATC 1 cut(s) 283
BspQI GCTCTTC 1 cut(s) 1048
BspT107I GGYRCC 2 cut(s) 42, 701
BsrDI GCAATG 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 3 cut(s) 43, 174, 190
BssECI CCNNGG 6 cut(s) 157, 583, 705, 859, 896, 1048
BssMI GATC 4 cut(s) 275, 304, 430, 1039
BssNI GRCGYC 1 cut(s) 599
BssT1I CCWWGG 2 cut(s) 157, 705
Bst2UI CCWGG 3 cut(s) 585, 897, 1050
Bst4CI ACNGT 4 cut(s) 267, 485, 927, 977
Bst6I CTCTTC 1 cut(s) 1048
BstACI GRCGYC 1 cut(s) 599
BstAPI GCANNNNNTGC 1 cut(s) 847
BstAUI TGTACA 1 cut(s) 720
BstC8I GCNNGC 2 cut(s) 210, 992
BstDEI CTNAG 3 cut(s) 17, 192, 465
BstDSI CCRYGG 1 cut(s) 859
BstF5I GGATG 4 cut(s) 217, 337, 358, 952
BstKTI GATC 4 cut(s) 278, 307, 433, 1042
BstMBI GATC 4 cut(s) 275, 304, 430, 1039
BstMWI GCNNNNNNNGC 3 cut(s) 497, 710, 847
BstNI CCWGG 3 cut(s) 585, 897, 1050
BstPAI GACNNNNGTC 1 cut(s) 447
BstSCI CCNGG 3 cut(s) 583, 895, 1048
BstSFI CTRYAG 1 cut(s) 1112
BstSLI GKGCMC 1 cut(s) 568
BstV1I GCAGC 2 cut(s) 192, 931
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
Bsu15I ATCGAT 1 cut(s) 1077
BsuRI GGCC 2 cut(s) 173, 405
BsuTUI ATCGAT 1 cut(s) 1077
BtgI CCRYGG 1 cut(s) 859
BtgZI GCGATG 1 cut(s) 752
BtrI CACGTC 2 cut(s) 569, 698
BtsCI GGATG 4 cut(s) 217, 337, 358, 952
BtsIMutI CAGTG 3 cut(s) 181, 285, 723
Cac8I GCNNGC 2 cut(s) 210, 992
Cfr13I GGNCC 2 cut(s) 171, 403
ClaI ATCGAT 1 cut(s) 1077
CseI GACGC 1 cut(s) 677
Csp6I GTAC 3 cut(s) 481, 721, 776
CviAII CATG 2 cut(s) 377, 773
CviQI GTAC 3 cut(s) 481, 721, 776
DdeI CTNAG 3 cut(s) 17, 192, 465
DpnI GATC 4 cut(s) 277, 306, 432, 1041
DpnII GATC 4 cut(s) 275, 304, 430, 1039
DraI TTTAAA 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 1048
EarI CTCTTC 1 cut(s) 1048
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 157, 705
Eco24I GRGCYC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 2 cut(s) 756, 1083
Eco88I CYCGRG 2 cut(s) 1002, 1142
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 403
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 583, 895, 1048
EcoT14I CCWWGG 2 cut(s) 157, 705
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 2 cut(s) 157, 705
FaeI CATG 2 cut(s) 380, 776
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 1 cut(s) 807
FatI CATG 2 cut(s) 376, 772
FblI GTMKAC 1 cut(s) 451
Fnu4HI GCNGC 2 cut(s) 206, 920
FokI GGATG 4 cut(s) 204, 344, 365, 959
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 2 cut(s) 206, 920
FspBI CTAG 1 cut(s) 572
GluI GCNGC 2 cut(s) 206, 920
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 2 cut(s) 173, 405
HapII CCGG 1 cut(s) 779
HgaI GACGC 1 cut(s) 677
Hin1I GRCGYC 1 cut(s) 599
Hin1II CATG 2 cut(s) 380, 776
HinfI GANTC 2 cut(s) 1044, 1117
HpaII CCGG 1 cut(s) 779
Hpy166II GTNNAC 4 cut(s) 371, 452, 566, 723
Hpy188I TCNGA 4 cut(s) 193, 346, 466, 597
Hpy188III TCNNGA 3 cut(s) 302, 1037, 1142
Hpy8I GTNNAC 4 cut(s) 371, 452, 566, 723
Hpy99I CGWCG 1 cut(s) 601
HpyAV CCTTC 4 cut(s) 46, 91, 416, 731
HpyCH4III ACNGT 4 cut(s) 267, 485, 927, 977
HpyCH4IV ACGT 3 cut(s) 568, 599, 697
HpyCH4V TGCA 8 cut(s) 11, 92, 109, 356, 511, 566, 868, 919
HpyF10VI GCNNNNNNNGC 3 cut(s) 497, 710, 847
HpyF3I CTNAG 3 cut(s) 17, 192, 465
HpySE526I ACGT 3 cut(s) 568, 599, 697
Hsp92I GRCGYC 1 cut(s) 599
Hsp92II CATG 2 cut(s) 380, 776
Kzo9I GATC 4 cut(s) 275, 304, 430, 1039
LguI GCTCTTC 1 cut(s) 1048
LmnI GCTCC 4 cut(s) 26, 689, 898, 1094
Lsp1109I GCAGC 2 cut(s) 192, 931
LweI GCATC 3 cut(s) 322, 343, 850
MaeI CTAG 1 cut(s) 572
MaeII ACGT 3 cut(s) 568, 599, 697
MaeIII GTNAC 1 cut(s) 820
MalI GATC 4 cut(s) 277, 306, 432, 1041
MboI GATC 4 cut(s) 275, 304, 430, 1039
MboII GAAGA 4 cut(s) 76, 485, 962, 1065
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 4 cut(s) 8, 502, 568, 1151
MmeI TCCRAC 2 cut(s) 620, 948
MseI TTAA 6 cut(s) 239, 419, 558, 630, 1122, 1180
MslI CAYNNNNRTG 1 cut(s) 771
MspI CCGG 1 cut(s) 779
MspR9I CCNGG 3 cut(s) 585, 897, 1050
MvaI CCWGG 3 cut(s) 585, 897, 1050
MwoI GCNNNNNNNGC 3 cut(s) 497, 710, 847
NdeII GATC 4 cut(s) 275, 304, 430, 1039
NlaIII CATG 2 cut(s) 380, 776
NlaIV GGNNCC 4 cut(s) 44, 172, 703, 894
PaeR7I CTCGAG 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 1048
PfeI GAWTC 2 cut(s) 1044, 1117
PkrI GCNGC 2 cut(s) 207, 921
PshAI GACNNNNGTC 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 3 cut(s) 583, 895, 1048
PspGI CCWGG 3 cut(s) 583, 895, 1048
PspN4I GGNNCC 4 cut(s) 44, 172, 703, 894
PspPI GGNCC 2 cut(s) 171, 403
PspXI VCTCGAGB 1 cut(s) 1002
PsuI RGATCY 1 cut(s) 304
RsaI GTAC 3 cut(s) 482, 722, 777
RsaNI GTAC 3 cut(s) 481, 721, 776
RseI CAYNNNNRTG 1 cut(s) 771
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 1048
SaqAI TTAA 6 cut(s) 239, 419, 558, 630, 1122, 1180
SatI GCNGC 2 cut(s) 206, 920
Sau3AI GATC 4 cut(s) 275, 304, 430, 1039
Sau96I GGNCC 2 cut(s) 171, 403
ScrFI CCNGG 3 cut(s) 585, 897, 1050
SduI GDGCHC 4 cut(s) 8, 502, 568, 1151
SfaNI GCATC 3 cut(s) 322, 343, 850
SfcI CTRYAG 1 cut(s) 1112
Sfr274I CTCGAG 1 cut(s) 1002
SlaI CTCGAG 1 cut(s) 1002
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 1002
SmoI CTYRAG 1 cut(s) 1002
SsiI CCGC 1 cut(s) 839
SspMI CTAG 1 cut(s) 572
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 583, 895, 1048
StyI CCWWGG 2 cut(s) 157, 705
TaaI ACNGT 4 cut(s) 267, 485, 927, 977
TaiI ACGT 3 cut(s) 571, 602, 700
TaqI TCGA 3 cut(s) 1003, 1042, 1077
TatI WGTACW 1 cut(s) 720
TfiI GAWTC 2 cut(s) 1044, 1117
Tru1I TTAA 6 cut(s) 239, 419, 558, 630, 1122, 1180
Tru9I TTAA 6 cut(s) 239, 419, 558, 630, 1122, 1180
TscAI CASTG 3 cut(s) 181, 285, 730
TseI GCWGC 2 cut(s) 205, 919
TspDTI ATGAA 3 cut(s) 486, 963, 1012
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 3 cut(s) 181, 285, 730
VneI GTGCAC 1 cut(s) 564
XapI RAATTY 4 cut(s) 67, 121, 715, 953
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 1002
XmiI GTMKAC 1 cut(s) 451
XspI CTAG 1 cut(s) 572
ZraI GACGTC 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.