RLG00000014536

N-acetyl-gamma-glutamyl-phosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007780
Physical Location & Seq
Forward (+)
54456778 .. 54462049
5272 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014536

Sequence Viewer

Length: 1182 bp
ATGAGCTCTGCAACTCTCAGCTCCAATTTTCTCCAAACTGGGTGCCAATGGAAGGATGGAGTGAAGAATTTAGAGTTTAATAAGAGAAATGCAGTGAAGCTGTTTGTGAAATGTTCTGTGAATTCAAAAACCCAGAAAGCAGAAAAGGAAGTTCGCCTTGGTGTTCTTGGAGCCAGTGGCTACACTGGTTCTGAGATTGTTCGGCTGCTGGCAAATCATCCTCACTTTGGCATTACCTTAATGACTGCTGATAGAAAAGCTGGACAGTCAATTGCATCGGTGTTTCCTCATCTAATTTCCCAAAATCTGCCAAAGATGGTTGCTGTTAAGGATGCCGATTTTTCTGATGTGGATGCAGTATTTTGTTGTTTACCACATGGAACAACACAGGAAATTATCAAAGGCCTTCCAAAGAGTTTAAAGATTGTTGATCTGTCTGCTGACTTCCGTCTACAAGATATATCTGAGTATGAAGAATGGTATGGTCAGGCACATAGAGCGCCGGATTTGCAGAAAGAAGCTGTGTATGGTTTGACAGAGATTTTGAGGGAGGAGATTAAAAGTGCGCGTCTAGTTTCTAACCCTGGCTGTTACCCGACTTCTATTCAGCTTCCCCTTGTTCCATTGTTTAAGGCTAATCTTATTCAATCTAAAAATATTATTATCGACTCAAAATCTGGTGTTAGTGGAGCAGGACGTGGTGCCAAGGAGGCTAATTTGTACACTGAAATAGCTGAAGGCATATATTCTTATGGCATTACCCGTCATCGTCATGTTCCGGAAATTGAGCAGGGACTATCTGGTGCTGCCAATTCAAAAGTAACTGTTAGTTTTACCCCACATCTAATGCCAATGAGCCGTGGTATGCAATCAACTATTTACGTGGAAATGGCTCCAGGGGTAGAAATCAAGGACTTGCGGCAACAGTTGAGGTTATCCTATGAGGATGAAGAATTTGTAGTTTTGTTGGAGGAAGGTGTTGTTCCTCGCACACATAATACTCGAGGATCTAATTATTGTTTCATCAATGTCTTTCCTGATCGAATCCCTGGAAGAGCAATAATAATTTCAGTTATCGATAATCTTGTCAAGGGAGCTTCAGGTCAGGCTCTACAGAATCTTAATTTGATGATGGGATTTCCAGAGAGCACAGGGCTTCTTTATCAGCCATTGTTTCCTTAA

Protein Analysis

394

Amino Acids

43.27

Weight (kDa)

7.64

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Semialdhyde_dh PF01118 53 - 189 6.6e-29 Semialdehyde dehydrogenase, NAD binding domain
Semialdhyde_dhC_1 PF22698 197 - 361 1.2e-57 Semialdehyde dehydrogenase, dimerisation domain
Semialdhyde_dhC PF02774 206 - 364 2.2e-20 Semialdehyde dehydrogenase, dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 42, 701
AccI GTMKAC 1 cut(s) 451
AccII CGCG 1 cut(s) 568
AccIII TCCGGA 1 cut(s) 778
AciI CCGC 1 cut(s) 919
AclWI GGATC 1 cut(s) 1015
AcsI RAATTY 3 cut(s) 67, 121, 953
AcuI CTGAAG 2 cut(s) 756, 1083
AfaI GTAC 1 cut(s) 722
AfiI CCNNNNNNNGG 2 cut(s) 52, 227
AgsI TTSAA 3 cut(s) 126, 647, 816
AjiI CACGTC 1 cut(s) 698
AjnI CCWGG 3 cut(s) 583, 895, 1048
AjuI GAANNNNNNNTTGG 2 cut(s) 141, 173
AluBI AGCT 8 cut(s) 6, 21, 100, 260, 521, 610, 734, 1097
AluI AGCT 8 cut(s) 6, 21, 100, 260, 521, 610, 734, 1097
Alw21I GWGCWC 2 cut(s) 8, 1151
AlwI GGATC 1 cut(s) 1015
Ama87I CYCGRG 1 cut(s) 1002
Aor13HI TCCGGA 1 cut(s) 778
AoxI GGCC 1 cut(s) 403
ApeKI GCWGC 2 cut(s) 205, 806
ApoI RAATTY 3 cut(s) 67, 121, 953
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
AspLEI GCGC 2 cut(s) 502, 568
AvaI CYCGRG 1 cut(s) 1002
BanI GGYRCC 2 cut(s) 42, 701
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 2 cut(s) 8, 1151
BbvI GCAGC 2 cut(s) 192, 793
BccI CCATC 3 cut(s) 50, 310, 1126
BceAI ACGGC 1 cut(s) 843
BciT130I CCWGG 3 cut(s) 585, 897, 1050
BfaI CTAG 1 cut(s) 572
BfmI CTRYAG 1 cut(s) 1112
BfoI RGCGCY 1 cut(s) 503
BglI GCCNNNNNGGC 1 cut(s) 710
BisI GCNGC 3 cut(s) 206, 807, 920
BlsI GCNGC 3 cut(s) 207, 808, 921
Bme1390I CCNGG 3 cut(s) 585, 897, 1050
BmeT110I CYCGRG 1 cut(s) 1002
BmgBI CACGTC 1 cut(s) 698
BmiI GGNNCC 4 cut(s) 44, 172, 703, 894
BmrFI CCNGG 3 cut(s) 585, 897, 1050
BmrI ACTGGG 1 cut(s) 48
BmsI GCATC 3 cut(s) 284, 322, 343
BmuI ACTGGG 1 cut(s) 48
BoxI GACNNNNGTC 1 cut(s) 447
BpmI CTGGAG 1 cut(s) 879
Bsa29I ATCGAT 1 cut(s) 1077
BsaAI YACGTR 1 cut(s) 883
BsaJI CCNNGG 6 cut(s) 157, 583, 705, 859, 896, 1048
BsaWI WCCGGW 1 cut(s) 778
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 227
Bse1I ACTGG 3 cut(s) 43, 174, 190
BseAI TCCGGA 1 cut(s) 778
BseBI CCWGG 3 cut(s) 585, 897, 1050
BseCI ATCGAT 1 cut(s) 1077
BseDI CCNNGG 6 cut(s) 157, 583, 705, 859, 896, 1048
BseGI GGATG 5 cut(s) 61, 217, 337, 358, 952
BseLI CCNNNNNNNGG 2 cut(s) 52, 227
BseMII CTCAG 3 cut(s) 31, 183, 456
BseNI ACTGG 3 cut(s) 43, 174, 190
BseRI GAGGAG 1 cut(s) 566
BseXI GCAGC 2 cut(s) 192, 793
Bsh1236I CGCG 1 cut(s) 568
BshFI GGCC 1 cut(s) 405
BshNI GGYRCC 2 cut(s) 42, 701
BshVI ATCGAT 1 cut(s) 1077
BsiHKAI GWGCWC 2 cut(s) 8, 1151
BsiHKCI CYCGRG 1 cut(s) 1002
BsiSI CCGG 2 cut(s) 503, 779
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 2 cut(s) 52, 227
BsmFI GGGAC 1 cut(s) 807
BsnI GGCC 1 cut(s) 405
BsoBI CYCGRG 1 cut(s) 1002
Bsp1286I GDGCHC 2 cut(s) 8, 1151
Bsp13I TCCGGA 1 cut(s) 778
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 3 cut(s) 430, 1007, 1039
BspACI CCGC 1 cut(s) 919
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 3 cut(s) 30, 184, 457
BspDI ATCGAT 1 cut(s) 1077
BspEI TCCGGA 1 cut(s) 778
BspFNI CGCG 1 cut(s) 568
BspLI GGNNCC 4 cut(s) 44, 172, 703, 894
BspPI GGATC 1 cut(s) 1015
BspQI GCTCTTC 1 cut(s) 1048
BspT107I GGYRCC 2 cut(s) 42, 701
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 3 cut(s) 43, 174, 190
BssECI CCNNGG 6 cut(s) 157, 583, 705, 859, 896, 1048
BssMI GATC 3 cut(s) 430, 1007, 1039
BssT1I CCWWGG 2 cut(s) 157, 705
Bst2UI CCWGG 3 cut(s) 585, 897, 1050
Bst4CI ACNGT 3 cut(s) 267, 826, 927
Bst6I CTCTTC 1 cut(s) 1048
BstAUI TGTACA 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 883
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 3 cut(s) 17, 192, 465
BstDSI CCRYGG 1 cut(s) 859
BstF5I GGATG 5 cut(s) 61, 217, 337, 358, 952
BstFNI CGCG 1 cut(s) 568
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 2 cut(s) 502, 568
BstKTI GATC 3 cut(s) 433, 1010, 1042
BstMBI GATC 3 cut(s) 430, 1007, 1039
BstMWI GCNNNNNNNGC 3 cut(s) 497, 508, 710
BstNI CCWGG 3 cut(s) 585, 897, 1050
BstPAI GACNNNNGTC 1 cut(s) 447
BstSCI CCNGG 3 cut(s) 583, 895, 1048
BstSFI CTRYAG 1 cut(s) 1112
BstUI CGCG 1 cut(s) 568
BstV1I GCAGC 2 cut(s) 192, 793
BstX2I RGATCY 1 cut(s) 1007
BstYI RGATCY 1 cut(s) 1007
Bsu15I ATCGAT 1 cut(s) 1077
BsuRI GGCC 1 cut(s) 405
BsuTUI ATCGAT 1 cut(s) 1077
BtgI CCRYGG 1 cut(s) 859
BtrI CACGTC 1 cut(s) 698
BtsCI GGATG 5 cut(s) 61, 217, 337, 358, 952
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 4 cut(s) 99, 181, 183, 723
Cac8I GCNNGC 1 cut(s) 210
CfoI GCGC 2 cut(s) 502, 568
ClaI ATCGAT 1 cut(s) 1077
CseI GACGC 1 cut(s) 557
Csp6I GTAC 1 cut(s) 721
CviAII CATG 2 cut(s) 377, 773
CviQI GTAC 1 cut(s) 721
DdeI CTNAG 3 cut(s) 17, 192, 465
DpnI GATC 3 cut(s) 432, 1009, 1041
DpnII GATC 3 cut(s) 430, 1007, 1039
DraI TTTAAA 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 1048
EarI CTCTTC 1 cut(s) 1048
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 157, 705
Eco147I AGGCCT 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 2 cut(s) 756, 1083
Eco88I CYCGRG 1 cut(s) 1002
EcoICRI GAGCTC 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 583, 895, 1048
EcoT14I CCWWGG 2 cut(s) 157, 705
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 2 cut(s) 157, 705
FaeI CATG 2 cut(s) 380, 776
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 1 cut(s) 807
FatI CATG 2 cut(s) 376, 772
FblI GTMKAC 1 cut(s) 451
Fnu4HI GCNGC 3 cut(s) 206, 807, 920
FokI GGATG 5 cut(s) 68, 204, 344, 365, 959
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 3 cut(s) 206, 807, 920
FspBI CTAG 1 cut(s) 572
GlaI GCGC 2 cut(s) 501, 567
GluI GCNGC 3 cut(s) 206, 807, 920
GsuI CTGGAG 1 cut(s) 879
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 1 cut(s) 405
HapII CCGG 2 cut(s) 503, 779
HgaI GACGC 1 cut(s) 557
HhaI GCGC 2 cut(s) 502, 568
Hin1II CATG 2 cut(s) 380, 776
Hin6I GCGC 2 cut(s) 500, 566
HinP1I GCGC 2 cut(s) 500, 566
HinfI GANTC 3 cut(s) 668, 1044, 1117
HpaII CCGG 2 cut(s) 503, 779
Hpy166II GTNNAC 3 cut(s) 371, 452, 723
Hpy188I TCNGA 3 cut(s) 193, 346, 466
Hpy188III TCNNGA 3 cut(s) 779, 1037, 1142
Hpy8I GTNNAC 3 cut(s) 371, 452, 723
HpyAV CCTTC 4 cut(s) 46, 416, 731, 968
HpyCH4III ACNGT 3 cut(s) 267, 826, 927
HpyCH4IV ACGT 2 cut(s) 697, 882
HpyCH4V TGCA 6 cut(s) 11, 92, 275, 356, 511, 868
HpyF10VI GCNNNNNNNGC 3 cut(s) 497, 508, 710
HpyF3I CTNAG 3 cut(s) 17, 192, 465
HpySE526I ACGT 2 cut(s) 697, 882
Hsp92II CATG 2 cut(s) 380, 776
HspAI GCGC 2 cut(s) 500, 566
Kpn2I TCCGGA 1 cut(s) 778
Kzo9I GATC 3 cut(s) 430, 1007, 1039
LguI GCTCTTC 1 cut(s) 1048
LmnI GCTCC 5 cut(s) 26, 170, 689, 898, 1094
Lsp1109I GCAGC 2 cut(s) 192, 793
LweI GCATC 3 cut(s) 284, 322, 343
MaeI CTAG 1 cut(s) 572
MaeII ACGT 2 cut(s) 697, 882
MaeIII GTNAC 2 cut(s) 590, 820
MalI GATC 3 cut(s) 432, 1009, 1041
MboI GATC 3 cut(s) 430, 1007, 1039
MboII GAAGA 4 cut(s) 76, 485, 962, 1065
MfeI CAATTG 1 cut(s) 270
MflI RGATCY 1 cut(s) 1007
MhlI GDGCHC 2 cut(s) 8, 1151
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 1 cut(s) 948
MroI TCCGGA 1 cut(s) 778
MseI TTAA 8 cut(s) 78, 239, 327, 419, 558, 630, 1122, 1180
MslI CAYNNNNRTG 1 cut(s) 771
MspI CCGG 2 cut(s) 503, 779
MspR9I CCNGG 3 cut(s) 585, 897, 1050
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 3 cut(s) 585, 897, 1050
MvnI CGCG 1 cut(s) 568
MwoI GCNNNNNNNGC 3 cut(s) 497, 508, 710
NdeII GATC 3 cut(s) 430, 1007, 1039
NlaIII CATG 2 cut(s) 380, 776
NlaIV GGNNCC 4 cut(s) 44, 172, 703, 894
PaeR7I CTCGAG 1 cut(s) 1002
PceI AGGCCT 1 cut(s) 405
PciSI GCTCTTC 1 cut(s) 1048
PfeI GAWTC 2 cut(s) 1044, 1117
PkrI GCNGC 3 cut(s) 207, 808, 921
PleI GAGTC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 662
Ppu21I YACGTR 1 cut(s) 883
PshAI GACNNNNGTC 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 3 cut(s) 583, 895, 1048
PspGI CCWGG 3 cut(s) 583, 895, 1048
PspN4I GGNNCC 4 cut(s) 44, 172, 703, 894
PspXI VCTCGAGB 1 cut(s) 1002
PsuI RGATCY 1 cut(s) 1007
RsaI GTAC 1 cut(s) 722
RsaNI GTAC 1 cut(s) 721
RseI CAYNNNNRTG 1 cut(s) 771
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 1048
SaqAI TTAA 8 cut(s) 78, 239, 327, 419, 558, 630, 1122, 1180
SatI GCNGC 3 cut(s) 206, 807, 920
Sau3AI GATC 3 cut(s) 430, 1007, 1039
SchI GAGTC 1 cut(s) 662
ScrFI CCNGG 3 cut(s) 585, 897, 1050
SduI GDGCHC 2 cut(s) 8, 1151
SfaNI GCATC 3 cut(s) 284, 322, 343
SfcI CTRYAG 1 cut(s) 1112
Sfr274I CTCGAG 1 cut(s) 1002
SlaI CTCGAG 1 cut(s) 1002
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 1002
SmoI CTYRAG 1 cut(s) 1002
SseBI AGGCCT 1 cut(s) 405
SsiI CCGC 1 cut(s) 919
SspI AATATT 1 cut(s) 658
SspMI CTAG 1 cut(s) 572
SstI GAGCTC 1 cut(s) 8
StuI AGGCCT 1 cut(s) 405
StyD4I CCNGG 3 cut(s) 583, 895, 1048
StyI CCWWGG 2 cut(s) 157, 705
TaaI ACNGT 3 cut(s) 267, 826, 927
TaiI ACGT 2 cut(s) 700, 885
TaqI TCGA 4 cut(s) 666, 1003, 1042, 1077
TatI WGTACW 1 cut(s) 720
TauI GCSGC 1 cut(s) 922
TfiI GAWTC 2 cut(s) 1044, 1117
Tru1I TTAA 8 cut(s) 78, 239, 327, 419, 558, 630, 1122, 1180
Tru9I TTAA 8 cut(s) 78, 239, 327, 419, 558, 630, 1122, 1180
TscAI CASTG 4 cut(s) 99, 181, 190, 730
TseI GCWGC 2 cut(s) 205, 806
TspDTI ATGAA 3 cut(s) 486, 963, 1012
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 4 cut(s) 99, 181, 190, 730
XapI RAATTY 3 cut(s) 67, 121, 953
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 1002
XmiI GTMKAC 1 cut(s) 451
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.