FvH4_2g07850

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008742
Physical Location & Seq
Reverse (-)
6769522 .. 6772166
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07850.t2

Sequence Viewer

Length: 777 bp
ATGGGTACAACAAAAGACCCGACCGGAGATCAGAAAAGTCGCGTTGGCCCGTCCACTGCTCTGGCTTACCTCGATCCTCAATACTGGGATGAGCGGTTCACTACTGAAGAGCATTACGAGTGGCTCAAGGACTATTCCCACTTTCGGCACCTCATTCAATCCCATATCTCTCCAAACTCTTTTGTCTTGGAGCTGGGTTGTGGAAATTCACAGTTGTCTGAGGAATTGTATAACGATGGGTTTAAGGAAATAACATGCATTGATTTATCTGCTGTTGCGGTGGAGAACATGCAGAAGAAGTTGCTGTCTAAAGGTCTTAAAGAAATAAAAGTTCTGGAAGCTGACATGCTAGACCTGCCTTTTAGCAACGAGTGTTTTGATGTTGTAATTGAGAAAGGAACCATGGAGGTATTGTTTGTGGATAGTGGTGACCCATGGAATCCTCGGCCTGCAACGGTAACCAAGGTCATGAAGATGCTTGAGGGTATTCATAGGGTTTTGAAACCAGATGGTGTTTACATTTCAATTTCCTTTGGCCAGCCACATTTCAGGCGCCCTTTATTTGAGGCTCCACAGTTTACCTGGTCTGTAGAGTGGAGTACCTTTGGTGATGGATTTCACTATTTCTTCTATACATTGAAGAAGGGAAGAAGATCACCAGATGATAAACGATCAAGTGATGTGAAGTTAGAGAGGCCATCCATAAATCTTTATCAAGACGAATTAGAGGGTGAAGACTTTATTTTCCGAACCAACTTGGACGATCTCAGCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_31

Protein Analysis

259

Amino Acids

29.77

Weight (kDa)

5.01

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 58 - 179 2.5e-19 Methyltransferase domain
Methyltransf_25 PF13649 62 - 136 7.2e-14 Methyltransferase domain
Methyltransf_11 PF08241 63 - 174 3.2e-15 Methyltransferase domain
Methyltransf_12 PF08242 63 - 172 2.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 363
AccB1I GGYRCC 2 cut(s) 147, 552
AccBSI CCGCTC 1 cut(s) 94
AccII CGCG 1 cut(s) 42
AciI CCGC 2 cut(s) 94, 278
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 1 cut(s) 205
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 553
AfaI GTAC 2 cut(s) 7, 601
AfiI CCNNNNNNNGG 1 cut(s) 144
AgsI TTSAA 4 cut(s) 158, 502, 525, 640
AjnI CCWGG 1 cut(s) 581
AloI GAACNNNNNNTCC 2 cut(s) 80, 112
AluBI AGCT 3 cut(s) 193, 341, 771
AluI AGCT 3 cut(s) 193, 341, 771
AlwI GGATC 1 cut(s) 68
AoxI GGCC 4 cut(s) 46, 446, 535, 695
ApoI RAATTY 1 cut(s) 205
AspLEI GCGC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 4 cut(s) 440, 620, 648, 743
BalI TGGCCA 1 cut(s) 537
BanI GGYRCC 2 cut(s) 147, 552
BbsI GAAGAC 1 cut(s) 741
BccI CCATC 4 cut(s) 230, 503, 605, 706
BciT130I CCWGG 1 cut(s) 583
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 556
BfuAI ACCTGC 1 cut(s) 363
Bme1390I CCNGG 1 cut(s) 583
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 4 cut(s) 149, 400, 554, 570
BmrFI CCNGG 1 cut(s) 583
BmrI ACTGGG 1 cut(s) 94
BmsI GCATC 1 cut(s) 465
BmuI ACTGGG 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 110, 500
BsaHI GRCGYC 1 cut(s) 553
BsaJI CCNNGG 4 cut(s) 402, 434, 443, 462
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 89
BseBI CCWGG 1 cut(s) 583
BseDI CCNNGG 4 cut(s) 402, 434, 443, 462
BseGI GGATG 2 cut(s) 94, 698
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 89
BseYI CCCAGC 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 4 cut(s) 48, 448, 537, 697
BshNI GGYRCC 2 cut(s) 147, 552
BsiEI CGRYCG 1 cut(s) 24
BsiSI CCGG 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 144
BsnI GGCC 4 cut(s) 48, 448, 537, 697
Bsp143I GATC 5 cut(s) 28, 73, 653, 671, 763
Bsp19I CCATGG 2 cut(s) 402, 434
BspACI CCGC 2 cut(s) 94, 278
BspANI GGCC 4 cut(s) 48, 448, 537, 697
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 1 cut(s) 42
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 149, 400, 554, 570
BspMI ACCTGC 1 cut(s) 363
BspPI GGATC 1 cut(s) 68
BspQI GCTCTTC 1 cut(s) 102
BspT107I GGYRCC 2 cut(s) 147, 552
BsrBI CCGCTC 1 cut(s) 94
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 4 cut(s) 402, 434, 443, 462
BssMI GATC 5 cut(s) 28, 73, 653, 671, 763
BssNI GRCGYC 1 cut(s) 553
BssT1I CCWWGG 3 cut(s) 402, 434, 462
Bst2UI CCWGG 1 cut(s) 583
Bst4CI ACNGT 3 cut(s) 213, 457, 576
Bst6I CTCTTC 1 cut(s) 102
BstACI GRCGYC 1 cut(s) 553
BstC8I GCNNGC 2 cut(s) 450, 539
BstDEI CTNAG 2 cut(s) 219, 767
BstDSI CCRYGG 2 cut(s) 402, 434
BstEII GGTNACC 2 cut(s) 428, 457
BstF5I GGATG 2 cut(s) 94, 698
BstFNI CGCG 1 cut(s) 42
BstH2I RGCGCY 1 cut(s) 556
BstHHI GCGC 1 cut(s) 555
BstKTI GATC 5 cut(s) 31, 76, 656, 674, 766
BstMBI GATC 5 cut(s) 28, 73, 653, 671, 763
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNI CCWGG 1 cut(s) 583
BstNSI RCATGY 3 cut(s) 258, 292, 349
BstPI GGTNACC 2 cut(s) 428, 457
BstSCI CCNGG 1 cut(s) 581
BstSFI CTRYAG 1 cut(s) 588
BstUI CGCG 1 cut(s) 42
BstV2I GAAGAC 1 cut(s) 741
BstXI CCANNNNNNTGG 1 cut(s) 61
BsuRI GGCC 4 cut(s) 48, 448, 537, 697
BtgI CCRYGG 2 cut(s) 402, 434
BtsCI GGATG 2 cut(s) 94, 698
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 363
Cac8I GCNNGC 2 cut(s) 450, 539
CciI TCATGA 1 cut(s) 468
CfoI GCGC 1 cut(s) 555
Cfr13I GGNCC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 581
Csp6I GTAC 2 cut(s) 6, 600
CviAII CATG 6 cut(s) 255, 289, 346, 403, 435, 469
CviQI GTAC 2 cut(s) 6, 600
DdeI CTNAG 2 cut(s) 219, 767
DinI GGCGCC 1 cut(s) 554
DpnI GATC 5 cut(s) 30, 75, 655, 673, 765
DpnII GATC 5 cut(s) 28, 73, 653, 671, 763
EaeI YGGCCR 1 cut(s) 535
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 3 cut(s) 402, 434, 462
Eco57I CTGAAG 1 cut(s) 126
Eco91I GGTNACC 2 cut(s) 428, 457
EcoO65I GGTNACC 2 cut(s) 428, 457
EcoRII CCWGG 1 cut(s) 581
EcoT14I CCWWGG 3 cut(s) 402, 434, 462
EcoT22I ATGCAT 1 cut(s) 260
EgeI GGCGCC 1 cut(s) 554
EheI GGCGCC 1 cut(s) 554
ErhI CCWWGG 3 cut(s) 402, 434, 462
FaeI CATG 6 cut(s) 258, 292, 349, 406, 438, 472
FatI CATG 6 cut(s) 254, 288, 345, 402, 434, 468
FokI GGATG 2 cut(s) 101, 685
FspBI CTAG 1 cut(s) 350
GlaI GCGC 1 cut(s) 554
GsaI CCCAGC 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 556
HaeIII GGCC 4 cut(s) 48, 448, 537, 697
HapII CCGG 1 cut(s) 24
HhaI GCGC 1 cut(s) 555
Hin1I GRCGYC 1 cut(s) 553
Hin1II CATG 6 cut(s) 258, 292, 349, 406, 438, 472
Hin6I GCGC 1 cut(s) 553
HinP1I GCGC 1 cut(s) 553
HinfI GANTC 1 cut(s) 439
HpaII CCGG 1 cut(s) 24
HphI GGTGA 4 cut(s) 440, 620, 648, 743
Hpy166II GTNNAC 4 cut(s) 54, 99, 517, 579
Hpy188I TCNGA 3 cut(s) 33, 220, 749
Hpy188III TCNNGA 3 cut(s) 335, 469, 716
Hpy8I GTNNAC 4 cut(s) 54, 99, 517, 579
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 3 cut(s) 213, 457, 576
HpyCH4V TGCA 3 cut(s) 258, 292, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 2 cut(s) 219, 767
Hsp92I GRCGYC 1 cut(s) 553
Hsp92II CATG 6 cut(s) 258, 292, 349, 406, 438, 472
HspAI GCGC 1 cut(s) 553
KasI GGCGCC 1 cut(s) 552
Kzo9I GATC 5 cut(s) 28, 73, 653, 671, 763
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 2 cut(s) 190, 574
LweI GCATC 1 cut(s) 465
MabI ACCWGGT 1 cut(s) 581
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 2 cut(s) 428, 457
MalI GATC 5 cut(s) 30, 75, 655, 673, 765
MbiI CCGCTC 1 cut(s) 94
MboI GATC 5 cut(s) 28, 73, 653, 671, 763
MboII GAAGA 8 cut(s) 119, 307, 484, 619, 652, 660, 663, 746
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 5 cut(s) 205, 224, 387, 525, 722
MluNI TGGCCA 1 cut(s) 537
Mly113I GGCGCC 1 cut(s) 553
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 1 cut(s) 260
MscI TGGCCA 1 cut(s) 537
MseI TTAA 2 cut(s) 243, 318
MslI CAYNNNNRTG 1 cut(s) 473
Msp20I TGGCCA 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 771
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 583
MvaI CCWGG 1 cut(s) 583
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 355
NarI GGCGCC 1 cut(s) 553
NcoI CCATGG 2 cut(s) 402, 434
NdeII GATC 5 cut(s) 28, 73, 653, 671, 763
NlaIII CATG 6 cut(s) 258, 292, 349, 406, 438, 472
NlaIV GGNNCC 4 cut(s) 149, 400, 554, 570
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 428
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 3 cut(s) 258, 292, 349
PagI TCATGA 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 102
PfeI GAWTC 1 cut(s) 439
PluTI GGCGCC 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 581
PspEI GGTNACC 2 cut(s) 428, 457
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 1 cut(s) 581
PspN4I GGNNCC 4 cut(s) 149, 400, 554, 570
PspPI GGNCC 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 771
RsaI GTAC 2 cut(s) 7, 601
RsaNI GTAC 2 cut(s) 6, 600
RseI CAYNNNNRTG 1 cut(s) 473
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 2 cut(s) 243, 318
Sau3AI GATC 5 cut(s) 28, 73, 653, 671, 763
Sau96I GGNCC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 583
SexAI ACCWGGT 1 cut(s) 581
SfaNI GCATC 1 cut(s) 465
SfcI CTRYAG 1 cut(s) 588
SfoI GGCGCC 1 cut(s) 554
SmiMI CAYNNNNRTG 1 cut(s) 473
SmlI CTYRAG 2 cut(s) 125, 479
SmoI CTYRAG 2 cut(s) 125, 479
Sse9I AATT 5 cut(s) 205, 224, 387, 525, 722
SsiI CCGC 2 cut(s) 94, 278
SspDI GGCGCC 1 cut(s) 552
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 1 cut(s) 581
StyI CCWWGG 3 cut(s) 402, 434, 462
TaaI ACNGT 3 cut(s) 213, 457, 576
TaqI TCGA 1 cut(s) 72
TasI AATT 5 cut(s) 205, 224, 387, 525, 722
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 2 cut(s) 243, 318
Tru9I TTAA 2 cut(s) 243, 318
TscAI CASTG 1 cut(s) 61
TseFI GTSAC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 2 cut(s) 479, 485
TspRI CASTG 1 cut(s) 61
XapI RAATTY 1 cut(s) 205
XceI RCATGY 3 cut(s) 258, 292, 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XspI CTAG 1 cut(s) 350
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.