RchiOBHm_Chr6g0262271

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008742
Physical Location & Seq
Reverse (-)
16964932 .. 16968838
3907 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23519

Sequence Viewer

Length: 771 bp
ATGGGTACAACAAAAGACCCGAACGGAGCTCAGAAAAGTCGCGTTGGCCCGACCACTACTCTGGCTTACCTCGATCCTCAATACTGGGATGAGCGCTTCACTACTGAGGAACATTACGAGTGGCTCAAGGACTATTCTCACTTTCGGCACCTCATTCAATCCCATCTCAATCCCAGTTCTTCTATCTTGGAGCTGGGTTGTGGAAATTCACAGTTGTGTGAGGAATTATATAAAGATGGGTTTACTGAAATAACATGCATTGATCTATCTGCTGTTGCGGTGGAGAACATGCAGAAGAAGTTACTGTCTAAAGGTCTTAAGGAAATAAAAGTTCTGGAAGCTGACATGCTAGACCTGCCTTTTAGCAACGAGTGTTTTGATGTTGTTATTGAGAAAGGAACCATGGAGGTATTGTTCGTGGATAGTGGTGACCCATGGAATCCCCGGCCTGCAACGGTAACCAAGGTCATGAAGATGCTTGAGGGCATTCATAGAGTTTTGAAACCAGATGGAATATACATTTCAATTTCTTTTGGCCAGCCACATTTCAGGCGCCCTTTATTTGAGGCTCCACAGTTTACCTGGTCTGTAGAGTGGAGTACCTTTGGTGATGGATTTCACTATTTCTTCTATACATTGAAGAAGGGAAGAAGATCACCAGATAATAGAAGTGATGAGAAGTTAGAGAGGCCATCCATAAATCTTTATCAAGATGAATTAGAGGGCGAAGACTTCATTTTCCGAACCAATTTGGATGAGCTCAGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_31

Protein Analysis

256

Amino Acids

29.57

Weight (kDa)

5.02

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 58 - 179 8.6e-20 Methyltransferase domain
Methyltransf_25 PF13649 62 - 171 1.1e-14 Methyltransferase domain
Methyltransf_11 PF08241 63 - 174 3.8e-15 Methyltransferase domain
Methyltransf_12 PF08242 63 - 172 3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 363
AccB1I GGYRCC 2 cut(s) 147, 552
AccII CGCG 1 cut(s) 42
AciI CCGC 1 cut(s) 278
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 1 cut(s) 205
AcyI GRCGYC 1 cut(s) 553
AfaI GTAC 2 cut(s) 7, 601
AfeI AGCGCT 1 cut(s) 95
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 4 cut(s) 158, 502, 525, 640
AjnI CCWGG 1 cut(s) 581
AleI CACNNNNGTG 1 cut(s) 214
AloI GAACNNNNNNTCC 2 cut(s) 398, 430
AluBI AGCT 5 cut(s) 29, 193, 341, 760, 765
AluI AGCT 5 cut(s) 29, 193, 341, 760, 765
Alw21I GWGCWC 2 cut(s) 31, 762
AlwI GGATC 1 cut(s) 68
Aor51HI AGCGCT 1 cut(s) 95
AoxI GGCC 4 cut(s) 46, 446, 535, 689
ApoI RAATTY 1 cut(s) 205
AspLEI GCGC 2 cut(s) 96, 555
AspS9I GGNCC 1 cut(s) 47
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 3 cut(s) 440, 620, 648
BalI TGGCCA 1 cut(s) 537
BanI GGYRCC 2 cut(s) 147, 552
BanII GRGCYC 2 cut(s) 31, 762
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 2 cut(s) 31, 762
BccI CCATC 5 cut(s) 171, 230, 503, 605, 700
BciT130I CCWGG 1 cut(s) 583
BcnI CCSGG 1 cut(s) 445
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 2 cut(s) 97, 556
BfrI CTTAAG 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 363
BlpI GCTNAGC 1 cut(s) 761
Bme1390I CCNGG 2 cut(s) 445, 583
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 4 cut(s) 149, 400, 554, 570
BmrFI CCNGG 2 cut(s) 445, 583
BmrI ACTGGG 2 cut(s) 94, 168
BmsI GCATC 1 cut(s) 465
BmuI ACTGGG 2 cut(s) 94, 168
BpiI GAAGAC 1 cut(s) 735
BplI GAGNNNNNCTC 1 cut(s) 749
Bpu1102I GCTNAGC 1 cut(s) 761
BpuEI CTTGAG 2 cut(s) 110, 500
BpuMI CCSGG 1 cut(s) 445
BsaHI GRCGYC 1 cut(s) 553
BsaJI CCNNGG 4 cut(s) 402, 434, 443, 462
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bse1I ACTGG 2 cut(s) 89, 174
BseBI CCWGG 1 cut(s) 583
BseDI CCNNGG 4 cut(s) 402, 434, 443, 462
BseGI GGATG 3 cut(s) 94, 692, 760
BseMII CTCAG 2 cut(s) 44, 96
BseNI ACTGG 2 cut(s) 89, 174
BseYI CCCAGC 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 4 cut(s) 48, 448, 537, 691
BshNI GGYRCC 2 cut(s) 147, 552
BsiHKAI GWGCWC 2 cut(s) 31, 762
BsiSI CCGG 1 cut(s) 445
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 4 cut(s) 48, 448, 537, 691
Bsp1286I GDGCHC 2 cut(s) 31, 762
Bsp143I GATC 3 cut(s) 73, 262, 653
Bsp1720I GCTNAGC 1 cut(s) 761
Bsp19I CCATGG 2 cut(s) 402, 434
BspACI CCGC 1 cut(s) 278
BspANI GGCC 4 cut(s) 48, 448, 537, 691
BspCNI CTCAG 2 cut(s) 43, 97
BspFNI CGCG 1 cut(s) 42
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 149, 400, 554, 570
BspMI ACCTGC 1 cut(s) 363
BspPI GGATC 1 cut(s) 68
BspT107I GGYRCC 2 cut(s) 147, 552
BspTI CTTAAG 1 cut(s) 317
BsrI ACTGG 2 cut(s) 89, 174
BssECI CCNNGG 4 cut(s) 402, 434, 443, 462
BssMI GATC 3 cut(s) 73, 262, 653
BssNI GRCGYC 1 cut(s) 553
BssT1I CCWWGG 3 cut(s) 402, 434, 462
Bst2UI CCWGG 1 cut(s) 583
Bst4CI ACNGT 4 cut(s) 213, 306, 457, 576
BstACI GRCGYC 1 cut(s) 553
BstAFI CTTAAG 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 450, 539
BstDEI CTNAG 4 cut(s) 30, 105, 761, 768
BstDSI CCRYGG 2 cut(s) 402, 434
BstEII GGTNACC 2 cut(s) 428, 457
BstF5I GGATG 3 cut(s) 94, 692, 760
BstFNI CGCG 1 cut(s) 42
BstH2I RGCGCY 2 cut(s) 97, 556
BstHHI GCGC 2 cut(s) 96, 555
BstKTI GATC 3 cut(s) 76, 265, 656
BstMBI GATC 3 cut(s) 73, 262, 653
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNI CCWGG 1 cut(s) 583
BstNSI RCATGY 3 cut(s) 258, 292, 349
BstPI GGTNACC 2 cut(s) 428, 457
BstSCI CCNGG 2 cut(s) 443, 581
BstSFI CTRYAG 1 cut(s) 588
BstUI CGCG 1 cut(s) 42
BstV2I GAAGAC 1 cut(s) 735
BstXI CCANNNNNNTGG 1 cut(s) 61
BsuRI GGCC 4 cut(s) 48, 448, 537, 691
BtgI CCRYGG 2 cut(s) 402, 434
BtsCI GGATG 3 cut(s) 94, 692, 760
BveI ACCTGC 1 cut(s) 363
Cac8I GCNNGC 2 cut(s) 450, 539
CciI TCATGA 1 cut(s) 468
CfoI GCGC 2 cut(s) 96, 555
Cfr13I GGNCC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 581
Csp6I GTAC 2 cut(s) 6, 600
CviAII CATG 6 cut(s) 255, 289, 346, 403, 435, 469
CviQI GTAC 2 cut(s) 6, 600
DdeI CTNAG 4 cut(s) 30, 105, 761, 768
DinI GGCGCC 1 cut(s) 554
DpnI GATC 3 cut(s) 75, 264, 655
DpnII GATC 3 cut(s) 73, 262, 653
EaeI YGGCCR 1 cut(s) 535
Ecl136II GAGCTC 2 cut(s) 29, 760
Eco130I CCWWGG 3 cut(s) 402, 434, 462
Eco24I GRGCYC 2 cut(s) 31, 762
Eco47III AGCGCT 1 cut(s) 95
Eco53kI GAGCTC 2 cut(s) 29, 760
Eco91I GGTNACC 2 cut(s) 428, 457
EcoICRI GAGCTC 2 cut(s) 29, 760
EcoO65I GGTNACC 2 cut(s) 428, 457
EcoRII CCWGG 1 cut(s) 581
EcoT14I CCWWGG 3 cut(s) 402, 434, 462
EcoT22I ATGCAT 1 cut(s) 260
EcoT38I GRGCYC 2 cut(s) 31, 762
EgeI GGCGCC 1 cut(s) 554
EheI GGCGCC 1 cut(s) 554
ErhI CCWWGG 3 cut(s) 402, 434, 462
FaeI CATG 6 cut(s) 258, 292, 349, 406, 438, 472
FatI CATG 6 cut(s) 254, 288, 345, 402, 434, 468
FokI GGATG 3 cut(s) 101, 679, 767
FriOI GRGCYC 2 cut(s) 31, 762
FspBI CTAG 1 cut(s) 350
GlaI GCGC 2 cut(s) 95, 554
GsaI CCCAGC 1 cut(s) 197
HaeII RGCGCY 2 cut(s) 97, 556
HaeIII GGCC 4 cut(s) 48, 448, 537, 691
HapII CCGG 1 cut(s) 445
HhaI GCGC 2 cut(s) 96, 555
Hin1I GRCGYC 1 cut(s) 553
Hin1II CATG 6 cut(s) 258, 292, 349, 406, 438, 472
Hin6I GCGC 2 cut(s) 94, 553
HinP1I GCGC 2 cut(s) 94, 553
HinfI GANTC 1 cut(s) 439
HpaII CCGG 1 cut(s) 445
HphI GGTGA 3 cut(s) 440, 620, 648
Hpy166II GTNNAC 2 cut(s) 243, 579
Hpy188I TCNGA 2 cut(s) 33, 743
Hpy188III TCNNGA 3 cut(s) 335, 469, 710
Hpy8I GTNNAC 2 cut(s) 243, 579
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 4 cut(s) 213, 306, 457, 576
HpyCH4V TGCA 3 cut(s) 258, 292, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 4 cut(s) 30, 105, 761, 768
Hsp92I GRCGYC 1 cut(s) 553
Hsp92II CATG 6 cut(s) 258, 292, 349, 406, 438, 472
HspAI GCGC 2 cut(s) 94, 553
KasI GGCGCC 1 cut(s) 552
Kzo9I GATC 3 cut(s) 73, 262, 653
LmnI GCTCC 3 cut(s) 26, 190, 574
LweI GCATC 1 cut(s) 465
MabI ACCWGGT 1 cut(s) 581
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 3 cut(s) 300, 428, 457
MalI GATC 3 cut(s) 75, 264, 655
MboI GATC 3 cut(s) 73, 262, 653
MboII GAAGA 8 cut(s) 171, 307, 484, 619, 652, 660, 663, 740
MhlI GDGCHC 2 cut(s) 31, 762
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 5 cut(s) 205, 224, 525, 716, 748
MluNI TGGCCA 1 cut(s) 537
Mly113I GGCGCC 1 cut(s) 553
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 1 cut(s) 260
MscI TGGCCA 1 cut(s) 537
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 2 cut(s) 214, 473
Msp20I TGGCCA 1 cut(s) 537
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 1 cut(s) 445
MspR9I CCNGG 2 cut(s) 445, 583
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 1 cut(s) 583
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 355
NarI GGCGCC 1 cut(s) 553
NciI CCSGG 1 cut(s) 445
NcoI CCATGG 2 cut(s) 402, 434
NdeII GATC 3 cut(s) 73, 262, 653
NlaIII CATG 6 cut(s) 258, 292, 349, 406, 438, 472
NlaIV GGNNCC 4 cut(s) 149, 400, 554, 570
NmuCI GTSAC 1 cut(s) 428
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 3 cut(s) 258, 292, 349
OliI CACNNNNGTG 1 cut(s) 214
PagI TCATGA 1 cut(s) 468
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 1 cut(s) 439
PluTI GGCGCC 1 cut(s) 556
Psp124BI GAGCTC 2 cut(s) 31, 762
Psp6I CCWGG 1 cut(s) 581
PspEI GGTNACC 2 cut(s) 428, 457
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 1 cut(s) 581
PspN4I GGNNCC 4 cut(s) 149, 400, 554, 570
PspPI GGNCC 1 cut(s) 47
RsaI GTAC 2 cut(s) 7, 601
RsaNI GTAC 2 cut(s) 6, 600
RseI CAYNNNNRTG 2 cut(s) 214, 473
SacI GAGCTC 2 cut(s) 31, 762
SaqAI TTAA 1 cut(s) 318
Sau3AI GATC 3 cut(s) 73, 262, 653
Sau96I GGNCC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 445, 583
SduI GDGCHC 2 cut(s) 31, 762
SexAI ACCWGGT 1 cut(s) 581
SfaNI GCATC 1 cut(s) 465
SfcI CTRYAG 1 cut(s) 588
SfoI GGCGCC 1 cut(s) 554
SmiMI CAYNNNNRTG 2 cut(s) 214, 473
SmlI CTYRAG 3 cut(s) 125, 317, 479
SmoI CTYRAG 3 cut(s) 125, 317, 479
Sse9I AATT 5 cut(s) 205, 224, 525, 716, 748
SsiI CCGC 1 cut(s) 278
SspDI GGCGCC 1 cut(s) 552
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 2 cut(s) 31, 762
StyD4I CCNGG 2 cut(s) 443, 581
StyI CCWWGG 3 cut(s) 402, 434, 462
TaaI ACNGT 4 cut(s) 213, 306, 457, 576
TaqI TCGA 1 cut(s) 72
TasI AATT 5 cut(s) 205, 224, 525, 716, 748
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TseFI GTSAC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 4 cut(s) 479, 485, 724, 729
TspGWI ACGGA 1 cut(s) 39
Vha464I CTTAAG 1 cut(s) 317
XapI RAATTY 1 cut(s) 205
XceI RCATGY 3 cut(s) 258, 292, 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XspI CTAG 1 cut(s) 350
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.