pycom10g06540

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008742
Physical Location & Seq
Forward (+)
7640386 .. 7642983
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g06540.3

Sequence Viewer

Length: 753 bp
ATGAAATCCCAAAAAGCAAATTTAGCAGCTCAGCCCAAAAGCGGAAACATCCACCCCCCCAAAACACCCCCACACCAACCACCAAAACAAAACAAGGCAGACTGGAGGGGTTTTTCAGGGTTTATGGCCGGCGGTGAAGCTTCAACCGGCCAGAGTCTGAGTGGGAGGAGAAGTAAAACCCGAAACAGTGAAATGGGTACAAAGCAATACCCGACCGGAACCCAGAGAAATGGCGTTGGACCATCTACTGCTGCCGCCTACCTCGATCCCAAATACTGGGACGAACGATTCTCCTCTGAGGAGCACTACGAGTGGCTCAAGGACTACTCTCACTTTCGCCACCTCATCCAATCACATATCACTCCCAATTCCTCTGTGTTGGAGCTGGGTTGTGGAAACTCACAGTTGTCCGAAGAGTTGTATAAAGATGGGGTTACGGAAATAACATGCATTGATCTCTCCGCTGTTGCTGTAGAGAACATGCAGAAGAAGTTACAGTCTAAAGGTTTTAAAGAAATAAAAGTTCTGGAAGCTGACATGCTAGACCTGCCTTTTAGCAACGAGTGTTTTGACGTTGTTATTGAAAAAGGAACCATGGAGGTACTGTTCGTTGATAGTGGTGACCCATGGAATCCACGGCCTGCAACAGTAACCAAGGTCATGAAGATGCTTGAGGGTGTTGATAGGGTTCTGAAACCAGATGGCATTTACATCTCAATTTCTTTTGGACAGGGAAGAAGATCGATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_31

Protein Analysis

251

Amino Acids

27.76

Weight (kDa)

7.71

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 555
AccB7I CCANNNNNTGG 1 cut(s) 276
AciI CCGC 4 cut(s) 42, 132, 255, 462
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 2 cut(s) 126, 148
AcsI RAATTY 1 cut(s) 19
AfaI GTAC 2 cut(s) 199, 603
AfiI CCNNNNNNNGG 2 cut(s) 41, 276
AgsI TTSAA 2 cut(s) 144, 584
AloI GAACNNNNNNTCC 2 cut(s) 590, 622
AluBI AGCT 4 cut(s) 29, 140, 385, 533
AluI AGCT 4 cut(s) 29, 140, 385, 533
Alw21I GWGCWC 1 cut(s) 306
AlwI GGATC 1 cut(s) 260
AlwNI CAGNNNCTG 1 cut(s) 157
AoxI GGCC 3 cut(s) 126, 148, 638
ApeKI GCWGC 2 cut(s) 26, 251
ApoI RAATTY 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 146, 632
AvaII GGWCC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 2 cut(s) 38, 238
BccI CCATC 3 cut(s) 250, 422, 695
BceAI ACGGC 1 cut(s) 653
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 471
BfuAI ACCTGC 1 cut(s) 555
BisI GCNGC 3 cut(s) 27, 252, 255
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 3 cut(s) 28, 253, 256
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 220, 592
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 1 cut(s) 657
BmuI ACTGGG 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 124
Bpu1102I GCTNAGC 1 cut(s) 30
BpuEI CTTGAG 2 cut(s) 302, 692
Bsa29I ATCGAT 1 cut(s) 743
BsaJI CCNNGG 4 cut(s) 594, 626, 635, 654
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 590, 620
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 276
Bse118I RCCGGY 2 cut(s) 128, 146
Bse1I ACTGG 2 cut(s) 107, 281
BseCI ATCGAT 1 cut(s) 743
BseDI CCNNGG 4 cut(s) 594, 626, 635, 654
BseGI GGATG 2 cut(s) 48, 345
BseLI CCNNNNNNNGG 2 cut(s) 41, 276
BseMII CTCAG 3 cut(s) 44, 149, 288
BseNI ACTGG 2 cut(s) 107, 281
BseRI GAGGAG 3 cut(s) 181, 283, 314
BseXI GCAGC 2 cut(s) 38, 238
BseYI CCCAGC 1 cut(s) 385
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 3 cut(s) 128, 150, 640
BshVI ATCGAT 1 cut(s) 743
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 3 cut(s) 129, 147, 216
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 41, 276
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 3 cut(s) 128, 150, 640
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 3 cut(s) 265, 454, 740
Bsp1720I GCTNAGC 1 cut(s) 30
Bsp19I CCATGG 2 cut(s) 594, 626
BspACI CCGC 4 cut(s) 42, 132, 255, 462
BspANI GGCC 3 cut(s) 128, 150, 640
BspCNI CTCAG 3 cut(s) 43, 150, 289
BspDI ATCGAT 1 cut(s) 743
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 2 cut(s) 220, 592
BspMI ACCTGC 1 cut(s) 555
BspPI GGATC 1 cut(s) 260
BsrFI RCCGGY 2 cut(s) 128, 146
BsrI ACTGG 2 cut(s) 107, 281
BssAI RCCGGY 2 cut(s) 128, 146
BssECI CCNNGG 4 cut(s) 594, 626, 635, 654
BssMI GATC 3 cut(s) 265, 454, 740
BssT1I CCWWGG 3 cut(s) 594, 626, 654
Bst4CI ACNGT 5 cut(s) 188, 405, 498, 606, 649
Bst6I CTCTTC 1 cut(s) 408
BstC8I GCNNGC 2 cut(s) 130, 642
BstDEI CTNAG 3 cut(s) 30, 158, 297
BstDSI CCRYGG 3 cut(s) 594, 626, 635
BstEII GGTNACC 1 cut(s) 620
BstF5I GGATG 2 cut(s) 48, 345
BstKTI GATC 3 cut(s) 268, 457, 743
BstMBI GATC 3 cut(s) 265, 454, 740
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 2 cut(s) 23, 547
BstNSI RCATGY 3 cut(s) 450, 484, 541
BstPI GGTNACC 1 cut(s) 620
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 2 cut(s) 38, 238
BstXI CCANNNNNNTGG 1 cut(s) 230
Bsu15I ATCGAT 1 cut(s) 743
BsuRI GGCC 3 cut(s) 128, 150, 640
BsuTUI ATCGAT 1 cut(s) 743
BtgI CCRYGG 3 cut(s) 594, 626, 635
BtsCI GGATG 2 cut(s) 48, 345
BtsIMutI CAGTG 1 cut(s) 193
BveI ACCTGC 1 cut(s) 555
Cac8I GCNNGC 2 cut(s) 130, 642
CaiI CAGNNNCTG 1 cut(s) 157
CciI TCATGA 1 cut(s) 660
Cfr10I RCCGGY 2 cut(s) 128, 146
Cfr13I GGNCC 1 cut(s) 239
ClaI ATCGAT 1 cut(s) 743
Csp6I GTAC 2 cut(s) 198, 602
CviAII CATG 6 cut(s) 447, 481, 538, 595, 627, 661
CviJI RGCY 9 cut(s) 29, 34, 128, 140, 150, 316, 385, 533, 640
CviKI_1 RGCY 9 cut(s) 29, 34, 128, 140, 150, 316, 385, 533, 640
CviQI GTAC 2 cut(s) 198, 602
DdeI CTNAG 3 cut(s) 30, 158, 297
DpnI GATC 3 cut(s) 267, 456, 742
DpnII GATC 3 cut(s) 265, 454, 740
DraI TTTAAA 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 126, 148
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco130I CCWWGG 3 cut(s) 594, 626, 654
Eco47I GGWCC 1 cut(s) 239
Eco91I GGTNACC 1 cut(s) 620
EcoO65I GGTNACC 1 cut(s) 620
EcoT14I CCWWGG 3 cut(s) 594, 626, 654
EcoT22I ATGCAT 1 cut(s) 452
ErhI CCWWGG 3 cut(s) 594, 626, 654
FaeI CATG 6 cut(s) 450, 484, 541, 598, 630, 664
FaiI YATR 9 cut(s) 125, 357, 423, 448, 482, 539, 596, 628, 662
FaqI GGGAC 1 cut(s) 293
FatI CATG 6 cut(s) 446, 480, 537, 594, 626, 660
Fnu4HI GCNGC 3 cut(s) 27, 252, 255
FokI GGATG 2 cut(s) 35, 332
Fsp4HI GCNGC 3 cut(s) 27, 252, 255
FspBI CTAG 1 cut(s) 542
GluI GCNGC 3 cut(s) 27, 252, 255
GsaI CCCAGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 3 cut(s) 128, 150, 640
HapII CCGG 3 cut(s) 129, 147, 216
Hin1II CATG 6 cut(s) 450, 484, 541, 598, 630, 664
HindIII AAGCTT 1 cut(s) 138
HinfI GANTC 3 cut(s) 154, 288, 631
HpaII CCGG 3 cut(s) 129, 147, 216
HphI GGTGA 2 cut(s) 146, 632
Hpy188I TCNGA 4 cut(s) 159, 298, 412, 693
Hpy188III TCNNGA 2 cut(s) 527, 661
HpyCH4III ACNGT 5 cut(s) 188, 405, 498, 606, 649
HpyCH4IV ACGT 1 cut(s) 573
HpyCH4V TGCA 3 cut(s) 450, 484, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 547
HpyF3I CTNAG 3 cut(s) 30, 158, 297
HpySE526I ACGT 1 cut(s) 573
Hsp92II CATG 6 cut(s) 450, 484, 541, 598, 630, 664
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Kzo9I GATC 3 cut(s) 265, 454, 740
LmnI GCTCC 2 cut(s) 301, 382
Lsp1109I GCAGC 2 cut(s) 38, 238
LweI GCATC 1 cut(s) 657
MaeI CTAG 1 cut(s) 542
MaeII ACGT 1 cut(s) 573
MaeIII GTNAC 4 cut(s) 433, 492, 620, 649
MalI GATC 3 cut(s) 267, 456, 742
MboI GATC 3 cut(s) 265, 454, 740
MboII GAAGA 5 cut(s) 425, 499, 676, 747, 750
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 3 cut(s) 19, 367, 717
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 2 cut(s) 217, 360
MnlI CCTC 9 cut(s) 99, 159, 272, 292, 304, 353, 382, 592, 667
Mph1103I ATGCAT 1 cut(s) 452
MroNI GCCGGC 1 cut(s) 128
MseI TTAA 1 cut(s) 510
MslI CAYNNNNRTG 1 cut(s) 665
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 3 cut(s) 129, 147, 216
MwoI GCNNNNNNNGC 2 cut(s) 23, 547
NaeI GCCGGC 1 cut(s) 130
NcoI CCATGG 2 cut(s) 594, 626
NdeII GATC 3 cut(s) 265, 454, 740
NgoMIV GCCGGC 1 cut(s) 128
NlaIII CATG 6 cut(s) 450, 484, 541, 598, 630, 664
NlaIV GGNNCC 2 cut(s) 220, 592
NmuCI GTSAC 1 cut(s) 620
NsiI ATGCAT 1 cut(s) 452
NspI RCATGY 3 cut(s) 450, 484, 541
PagI TCATGA 1 cut(s) 660
PdiI GCCGGC 1 cut(s) 130
PfeI GAWTC 2 cut(s) 288, 631
PflMI CCANNNNNTGG 1 cut(s) 276
PkrI GCNGC 3 cut(s) 28, 253, 256
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
PspEI GGTNACC 1 cut(s) 620
PspFI CCCAGC 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 220, 592
PspPI GGNCC 1 cut(s) 239
PstNI CAGNNNCTG 1 cut(s) 157
RsaI GTAC 2 cut(s) 199, 603
RsaNI GTAC 2 cut(s) 198, 602
RseI CAYNNNNRTG 1 cut(s) 665
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 3 cut(s) 27, 252, 255
Sau3AI GATC 3 cut(s) 265, 454, 740
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 163
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 1 cut(s) 657
SfcI CTRYAG 1 cut(s) 471
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 665
SmlI CTYRAG 2 cut(s) 317, 671
SmoI CTYRAG 2 cut(s) 317, 671
Sse9I AATT 3 cut(s) 19, 367, 717
SsiI CCGC 4 cut(s) 42, 132, 255, 462
SspMI CTAG 1 cut(s) 542
StyI CCWWGG 3 cut(s) 594, 626, 654
TaaI ACNGT 5 cut(s) 188, 405, 498, 606, 649
TaiI ACGT 1 cut(s) 576
TaqI TCGA 2 cut(s) 264, 743
TasI AATT 3 cut(s) 19, 367, 717
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 288, 631
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 1 cut(s) 620
TseI GCWGC 2 cut(s) 26, 251
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 2 cut(s) 17, 677
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 1 cut(s) 193
Van91I CCANNNNNTGG 1 cut(s) 276
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 1 cut(s) 19
XceI RCATGY 3 cut(s) 450, 484, 541
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XspI CTAG 1 cut(s) 542
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.