MD10G1083300.v1.1

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008742
Physical Location & Seq
Forward (+)
12211767 .. 12214573
2807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1083300.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGGTACAAAGCAAGACCCGACCGGAACCCAGAGAAATGGCGTTGGGCCATCTACTGCTCTCGCCTACCTGGATCCCAAATACTGGGACGAACGATTCTCCTCTGAGGAGCACTACGAGTGGCTCAAGGACTACTCTCACTTCCGCCACCTCATCCAATCTCATATCACTCCCAATTCCTCTGTGTTGGAGCTGGGTTGTGGAAACTCACACTTGTCCGAAGAGTTGTATAAAGATGGGGTTACGGAAATAACTTGCATTGATCTGTCCGCTGTTGCCGTAGAGAACATGCAGAAGAAGTTACAGTCTAAAGGTTTTAAAGAAATAAAAGTTCTGGAAGCTGACATGCTAGACCTGCCTTTTAGCAACGAGTGTTTTGACGTTGTTATTGAGAAAGGAACCATGGAGGTACTGTTCGTTGATAGCGGTGACCCGTGGAATCCACGACCTGCAACAGTAACCAAGGTCATGAAAATGCTTGAGGGTGTTCATAGGGTTCTGAAACCAGATGGCATTTACATCTCAATTTCTTTTGGACAGCCACATTTTAGGCGCCCTTTTTTCGATGCTCCACAATTTACCTGGTCTGTGGAGTGGAGTACCTTTGGTGATGGATTTCATTACTTCTTCTATACATTGAAGAAGGGAAGAAGATCAGTGGATGATAAAGGATCTAGCAAAGAGAAGTTAGAGACTCAGTCTATTTGTCTTTTTCAAGATGAGTTAGAGGGCGAAGATTATCTTTTCCGAACCAACTTTGATGAACTCAATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_31

Protein Analysis

259

Amino Acids

29.65

Weight (kDa)

5.07

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 50 - 183 8.5e-06 Spermine/spermidine synthase domain
MTS PF05175 55 - 130 3.6e-06 Methyltransferase small domain
Methyltransf_31 PF13847 57 - 179 1e-21 Methyltransferase domain
Ubie_methyltran PF01209 57 - 129 3.2e-06 ubiE/COQ5 methyltransferase family
Methyltransf_25 PF13649 62 - 171 2.6e-15 Methyltransferase domain
Methyltransf_11 PF08241 63 - 174 2.8e-15 Methyltransferase domain
Methyltransf_12 PF08242 63 - 172 3.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 363, 457
AccB1I GGYRCC 1 cut(s) 552
AccB7I CCANNNNNTGG 1 cut(s) 84
AciI CCGC 3 cut(s) 145, 270, 426
AclWI GGATC 3 cut(s) 68, 81, 679
AcyI GRCGYC 1 cut(s) 553
AfaI GTAC 3 cut(s) 7, 411, 601
AfiI CCNNNNNNNGG 1 cut(s) 84
AgsI TTSAA 2 cut(s) 640, 716
AjnI CCWGG 2 cut(s) 69, 581
AloI GAACNNNNNNTCC 2 cut(s) 398, 430
AluBI AGCT 2 cut(s) 193, 341
AluI AGCT 2 cut(s) 193, 341
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 686
AlwI GGATC 3 cut(s) 68, 81, 679
AoxI GGCC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 440, 620
BamHI GGATCC 1 cut(s) 73
BanI GGYRCC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 114
BccI CCATC 4 cut(s) 58, 230, 503, 605
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 2 cut(s) 71, 583
BcoDI GTCTC 1 cut(s) 686
BfaI CTAG 2 cut(s) 350, 675
BfoI RGCGCY 1 cut(s) 556
BfuAI ACCTGC 2 cut(s) 363, 457
Bme1390I CCNGG 2 cut(s) 71, 583
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 4 cut(s) 28, 75, 400, 554
BmrFI CCNGG 2 cut(s) 71, 583
BmrI ACTGGG 1 cut(s) 94
BmsI GCATC 1 cut(s) 556
BmuI ACTGGG 1 cut(s) 94
BpuEI CTTGAG 2 cut(s) 110, 500
BsaHI GRCGYC 1 cut(s) 553
BsaJI CCNNGG 3 cut(s) 402, 434, 462
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse1I ACTGG 1 cut(s) 89
BseBI CCWGG 2 cut(s) 71, 583
BseDI CCNNGG 3 cut(s) 402, 434, 462
BseGI GGATG 2 cut(s) 153, 667
BseLI CCNNNNNNNGG 1 cut(s) 84
BseMII CTCAG 2 cut(s) 96, 710
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 91, 122
BseYI CCCAGC 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 1 cut(s) 49
BshNI GGYRCC 1 cut(s) 552
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 114
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 84
BsmAI GTCTC 1 cut(s) 686
BsmFI GGGAC 1 cut(s) 101
BsnI GGCC 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 4 cut(s) 73, 262, 653, 671
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 3 cut(s) 145, 270, 426
BspANI GGCC 1 cut(s) 49
BspCNI CTCAG 2 cut(s) 97, 709
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 28, 75, 400, 554
BspMI ACCTGC 2 cut(s) 363, 457
BspPI GGATC 3 cut(s) 68, 81, 679
BspT107I GGYRCC 1 cut(s) 552
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 3 cut(s) 402, 434, 462
BssMI GATC 4 cut(s) 73, 262, 653, 671
BssNI GRCGYC 1 cut(s) 553
BssT1I CCWWGG 2 cut(s) 402, 462
Bst2UI CCWGG 2 cut(s) 71, 583
Bst4CI ACNGT 3 cut(s) 306, 414, 457
Bst6I CTCTTC 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 553
BstDEI CTNAG 2 cut(s) 105, 696
BstDSI CCRYGG 2 cut(s) 402, 434
BstEII GGTNACC 1 cut(s) 428
BstF5I GGATG 2 cut(s) 153, 667
BstH2I RGCGCY 1 cut(s) 556
BstHHI GCGC 1 cut(s) 555
BstKTI GATC 4 cut(s) 76, 265, 656, 674
BstMAI GTCTC 1 cut(s) 686
BstMBI GATC 4 cut(s) 73, 262, 653, 671
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNI CCWGG 2 cut(s) 71, 583
BstNSI RCATGY 2 cut(s) 292, 349
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 2 cut(s) 69, 581
BstX2I RGATCY 2 cut(s) 73, 671
BstXI CCANNNNNNTGG 1 cut(s) 38
BstYI RGATCY 2 cut(s) 73, 671
BsuRI GGCC 1 cut(s) 49
BtgI CCRYGG 2 cut(s) 402, 434
BtsCI GGATG 2 cut(s) 153, 667
BtsIMutI CAGTG 1 cut(s) 663
BveI ACCTGC 2 cut(s) 363, 457
CciI TCATGA 1 cut(s) 468
CfoI GCGC 1 cut(s) 555
Cfr13I GGNCC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 581
Csp6I GTAC 3 cut(s) 6, 410, 600
CviAII CATG 4 cut(s) 289, 346, 403, 469
CviJI RGCY 5 cut(s) 49, 124, 193, 341, 541
CviKI_1 RGCY 5 cut(s) 49, 124, 193, 341, 541
CviQI GTAC 3 cut(s) 6, 410, 600
DdeI CTNAG 2 cut(s) 105, 696
DinI GGCGCC 1 cut(s) 554
DpnI GATC 4 cut(s) 75, 264, 655, 673
DpnII GATC 4 cut(s) 73, 262, 653, 671
DraI TTTAAA 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
EciI GGCGGA 1 cut(s) 134
Eco130I CCWWGG 2 cut(s) 402, 462
Eco91I GGTNACC 1 cut(s) 428
EcoO65I GGTNACC 1 cut(s) 428
EcoRII CCWGG 2 cut(s) 69, 581
EcoT14I CCWWGG 2 cut(s) 402, 462
EgeI GGCGCC 1 cut(s) 554
EheI GGCGCC 1 cut(s) 554
ErhI CCWWGG 2 cut(s) 402, 462
FaeI CATG 4 cut(s) 292, 349, 406, 472
FaiI YATR 8 cut(s) 165, 231, 290, 347, 404, 470, 492, 633
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 101
FatI CATG 4 cut(s) 288, 345, 402, 468
FokI GGATG 2 cut(s) 140, 674
FspBI CTAG 2 cut(s) 350, 675
GlaI GCGC 1 cut(s) 554
GsaI CCCAGC 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 556
HaeIII GGCC 1 cut(s) 49
HapII CCGG 1 cut(s) 24
HhaI GCGC 1 cut(s) 555
Hin1I GRCGYC 1 cut(s) 553
Hin1II CATG 4 cut(s) 292, 349, 406, 472
Hin6I GCGC 1 cut(s) 553
HinP1I GCGC 1 cut(s) 553
HinfI GANTC 3 cut(s) 96, 439, 694
HpaII CCGG 1 cut(s) 24
HphI GGTGA 2 cut(s) 440, 620
Hpy188I TCNGA 4 cut(s) 106, 220, 501, 749
Hpy188III TCNNGA 3 cut(s) 335, 469, 716
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 3 cut(s) 306, 414, 457
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 3 cut(s) 258, 292, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 2 cut(s) 105, 696
HpySE526I ACGT 1 cut(s) 381
Hsp92I GRCGYC 1 cut(s) 553
Hsp92II CATG 4 cut(s) 292, 349, 406, 472
HspAI GCGC 1 cut(s) 553
KasI GGCGCC 1 cut(s) 552
Kzo9I GATC 4 cut(s) 73, 262, 653, 671
LmnI GCTCC 3 cut(s) 109, 190, 574
LweI GCATC 1 cut(s) 556
MabI ACCWGGT 1 cut(s) 581
MaeI CTAG 2 cut(s) 350, 675
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 4 cut(s) 241, 300, 428, 457
MalI GATC 4 cut(s) 75, 264, 655, 673
MboI GATC 4 cut(s) 73, 262, 653, 671
MboII GAAGA 7 cut(s) 233, 307, 619, 652, 660, 663, 746
MflI RGATCY 2 cut(s) 73, 671
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 3 cut(s) 175, 525, 575
Mly113I GGCGCC 1 cut(s) 553
MlyI GAGTC 1 cut(s) 688
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 7 cut(s) 100, 112, 161, 190, 400, 475, 721
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 1 cut(s) 473
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 2 cut(s) 71, 583
MvaI CCWGG 2 cut(s) 71, 583
MwoI GCNNNNNNNGC 1 cut(s) 355
NarI GGCGCC 1 cut(s) 553
NcoI CCATGG 1 cut(s) 402
NdeII GATC 4 cut(s) 73, 262, 653, 671
NlaIII CATG 4 cut(s) 292, 349, 406, 472
NlaIV GGNNCC 4 cut(s) 28, 75, 400, 554
NmuCI GTSAC 1 cut(s) 428
NspI RCATGY 2 cut(s) 292, 349
PagI TCATGA 1 cut(s) 468
PfeI GAWTC 2 cut(s) 96, 439
PflFI GACNNNGTC 1 cut(s) 697
PflMI CCANNNNNTGG 1 cut(s) 84
PleI GAGTC 1 cut(s) 688
PluTI GGCGCC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 688
Psp6I CCWGG 2 cut(s) 69, 581
PspEI GGTNACC 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 2 cut(s) 69, 581
PspN4I GGNNCC 4 cut(s) 28, 75, 400, 554
PspPI GGNCC 1 cut(s) 47
PsuI RGATCY 2 cut(s) 73, 671
PsyI GACNNNGTC 1 cut(s) 697
RsaI GTAC 3 cut(s) 7, 411, 601
RsaNI GTAC 3 cut(s) 6, 410, 600
RseI CAYNNNNRTG 1 cut(s) 473
SaqAI TTAA 1 cut(s) 318
Sau3AI GATC 4 cut(s) 73, 262, 653, 671
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 1 cut(s) 688
ScrFI CCNGG 2 cut(s) 71, 583
SduI GDGCHC 1 cut(s) 114
SexAI ACCWGGT 1 cut(s) 581
SfaNI GCATC 1 cut(s) 556
SfoI GGCGCC 1 cut(s) 554
SmiMI CAYNNNNRTG 1 cut(s) 473
SmlI CTYRAG 2 cut(s) 125, 479
SmoI CTYRAG 2 cut(s) 125, 479
Sse9I AATT 3 cut(s) 175, 525, 575
SsiI CCGC 3 cut(s) 145, 270, 426
SspDI GGCGCC 1 cut(s) 552
SspMI CTAG 2 cut(s) 350, 675
StyD4I CCNGG 2 cut(s) 69, 581
StyI CCWWGG 2 cut(s) 402, 462
TaaI ACNGT 3 cut(s) 306, 414, 457
TaiI ACGT 1 cut(s) 384
TaqI TCGA 1 cut(s) 564
TasI AATT 3 cut(s) 175, 525, 575
TfiI GAWTC 2 cut(s) 96, 439
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 1 cut(s) 663
TseFI GTSAC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 4 cut(s) 479, 485, 608, 777
TspGWI ACGGA 1 cut(s) 260
TspRI CASTG 1 cut(s) 663
Tth111I GACNNNGTC 1 cut(s) 697
Van91I CCANNNNNTGG 1 cut(s) 84
XceI RCATGY 2 cut(s) 292, 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 579
XspI CTAG 2 cut(s) 350, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.