Rroxscaffold_7G00207950

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008742
Physical Location & Seq
Reverse (-)
57915534 .. 57918416
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00207950.1

Sequence Viewer

Length: 642 bp
ATGGGTACAACAAAAGACCCGAACGGAGCTCAGAAAAGTCGCGTTGGCCCGACCACTACTCTGGCTTACCTCGATCCTCAATACTGGGATGAGCGCTTCACTACTGAGGAGCATTACGAGTGGCTCAAGGACTATTCTCACTTTCGGCACCTCATTCAATCCCACCTCAATCCCAATTCTTCTATCTTGGAGCTGGGTTGTGGAAATTCACAGTTGTGTGAGGAATTATATAAAGATGGGTTTGCTGAAATAACATGCATTGATCTATCTGCTGTTGCTGTGGAGAATATGCAGAAGAAGTTACTGTCTAAAGGTCTTAAAGAAATAAAAGTTCTGGAAGCTGACATGCTAGACCTGCCTTTTAGCAACGAGTGTTTTGATGTTGTTATTGAGAAAGGAACCATGCCACATTTCAGGCGCCCTTTATTTGAGGCTCCACAGTTTACCTGGTCTGTAGAGTGGAGTACCTTTGGTGATGGATTTCACTATTTCTTCTATACATTGAAGAAGGGAAGAAGATCACCAGATAATAAACGATCGAGTGATGAGAAGTTAGAGAGGCCATCCATAAATCTTTATCAAGATGAATTAGAGGGTGAAGACTTCATTTTCCGAACCAATTTGGATGAGCTCAGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_31

Protein Analysis

213

Amino Acids

24.76

Weight (kDa)

4.97

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 57 - 139 1.2e-15 Methyltransferase domain
Methyltransf_25 PF13649 62 - 141 6.1e-15 Methyltransferase domain
Methyltransf_11 PF08241 63 - 140 5.8e-13 Methyltransferase domain
Methyltransf_12 PF08242 63 - 140 1.5e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 363
AccB1I GGYRCC 2 cut(s) 147, 417
AccII CGCG 1 cut(s) 42
AclWI GGATC 1 cut(s) 68
AcsI RAATTY 1 cut(s) 205
AcyI GRCGYC 1 cut(s) 418
AfaI GTAC 2 cut(s) 7, 466
AfeI AGCGCT 1 cut(s) 95
AgsI TTSAA 2 cut(s) 158, 505
AjnI CCWGG 1 cut(s) 446
AleI CACNNNNGTG 1 cut(s) 214
AluBI AGCT 5 cut(s) 29, 193, 341, 631, 636
AluI AGCT 5 cut(s) 29, 193, 341, 631, 636
Alw21I GWGCWC 2 cut(s) 31, 633
AlwI GGATC 1 cut(s) 68
Aor51HI AGCGCT 1 cut(s) 95
AoxI GGCC 2 cut(s) 46, 560
ApoI RAATTY 1 cut(s) 205
AspLEI GCGC 2 cut(s) 96, 420
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 3 cut(s) 485, 513, 608
BanI GGYRCC 2 cut(s) 147, 417
BanII GRGCYC 2 cut(s) 31, 633
BbsI GAAGAC 1 cut(s) 606
Bbv12I GWGCWC 2 cut(s) 31, 633
BccI CCATC 3 cut(s) 230, 470, 571
BciT130I CCWGG 1 cut(s) 448
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 453
BfoI RGCGCY 2 cut(s) 97, 421
BfuAI ACCTGC 1 cut(s) 363
BlpI GCTNAGC 1 cut(s) 632
Bme1390I CCNGG 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 4 cut(s) 149, 400, 419, 435
BmrFI CCNGG 1 cut(s) 448
BmrI ACTGGG 1 cut(s) 94
BmuI ACTGGG 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 606
BplI GAGNNNNNCTC 1 cut(s) 620
Bpu1102I GCTNAGC 1 cut(s) 632
BpuEI CTTGAG 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 418
Bse1I ACTGG 1 cut(s) 89
BseBI CCWGG 1 cut(s) 448
BseGI GGATG 3 cut(s) 94, 563, 631
BseMII CTCAG 2 cut(s) 44, 96
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 122
BseYI CCCAGC 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 42
Bsh1285I CGRYCG 1 cut(s) 539
BshFI GGCC 2 cut(s) 48, 562
BshNI GGYRCC 2 cut(s) 147, 417
BsiEI CGRYCG 1 cut(s) 539
BsiHKAI GWGCWC 2 cut(s) 31, 633
BsnI GGCC 2 cut(s) 48, 562
Bsp1286I GDGCHC 2 cut(s) 31, 633
Bsp143I GATC 4 cut(s) 73, 262, 518, 536
Bsp1720I GCTNAGC 1 cut(s) 632
BspANI GGCC 2 cut(s) 48, 562
BspCNI CTCAG 2 cut(s) 43, 97
BspFNI CGCG 1 cut(s) 42
BspLI GGNNCC 4 cut(s) 149, 400, 419, 435
BspMI ACCTGC 1 cut(s) 363
BspPI GGATC 1 cut(s) 68
BspT107I GGYRCC 2 cut(s) 147, 417
BsrI ACTGG 1 cut(s) 89
BssMI GATC 4 cut(s) 73, 262, 518, 536
BssNI GRCGYC 1 cut(s) 418
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 3 cut(s) 213, 306, 441
BstACI GRCGYC 1 cut(s) 418
BstDEI CTNAG 4 cut(s) 30, 105, 632, 639
BstF5I GGATG 3 cut(s) 94, 563, 631
BstFNI CGCG 1 cut(s) 42
BstH2I RGCGCY 2 cut(s) 97, 421
BstHHI GCGC 2 cut(s) 96, 420
BstKTI GATC 4 cut(s) 76, 265, 521, 539
BstMBI GATC 4 cut(s) 73, 262, 518, 536
BstMCI CGRYCG 1 cut(s) 539
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNI CCWGG 1 cut(s) 448
BstNSI RCATGY 2 cut(s) 258, 349
BstSCI CCNGG 1 cut(s) 446
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 1 cut(s) 42
BstV2I GAAGAC 1 cut(s) 606
BstXI CCANNNNNNTGG 1 cut(s) 61
BsuRI GGCC 2 cut(s) 48, 562
BtsCI GGATG 3 cut(s) 94, 563, 631
BveI ACCTGC 1 cut(s) 363
CfoI GCGC 2 cut(s) 96, 420
Cfr13I GGNCC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 446
Csp6I GTAC 2 cut(s) 6, 465
CviAII CATG 3 cut(s) 255, 346, 403
CviQI GTAC 2 cut(s) 6, 465
DdeI CTNAG 4 cut(s) 30, 105, 632, 639
DinI GGCGCC 1 cut(s) 419
DpnI GATC 4 cut(s) 75, 264, 520, 538
DpnII GATC 4 cut(s) 73, 262, 518, 536
Ecl136II GAGCTC 2 cut(s) 29, 631
Eco24I GRGCYC 2 cut(s) 31, 633
Eco47III AGCGCT 1 cut(s) 95
Eco53kI GAGCTC 2 cut(s) 29, 631
EcoICRI GAGCTC 2 cut(s) 29, 631
EcoRII CCWGG 1 cut(s) 446
EcoT22I ATGCAT 1 cut(s) 260
EcoT38I GRGCYC 2 cut(s) 31, 633
EgeI GGCGCC 1 cut(s) 419
EheI GGCGCC 1 cut(s) 419
FaeI CATG 3 cut(s) 258, 349, 406
FaiI YATR 8 cut(s) 229, 231, 256, 290, 347, 404, 498, 569
FatI CATG 3 cut(s) 254, 345, 402
FokI GGATG 3 cut(s) 101, 550, 638
FriOI GRGCYC 2 cut(s) 31, 633
FspBI CTAG 1 cut(s) 350
GlaI GCGC 2 cut(s) 95, 419
GsaI CCCAGC 1 cut(s) 197
HaeII RGCGCY 2 cut(s) 97, 421
HaeIII GGCC 2 cut(s) 48, 562
HhaI GCGC 2 cut(s) 96, 420
Hin1I GRCGYC 1 cut(s) 418
Hin1II CATG 3 cut(s) 258, 349, 406
Hin6I GCGC 2 cut(s) 94, 418
HinP1I GCGC 2 cut(s) 94, 418
HphI GGTGA 3 cut(s) 485, 513, 608
Hpy166II GTNNAC 1 cut(s) 444
Hpy188I TCNGA 2 cut(s) 33, 614
Hpy188III TCNNGA 2 cut(s) 335, 581
Hpy8I GTNNAC 1 cut(s) 444
HpyAV CCTTC 1 cut(s) 502
HpyCH4III ACNGT 3 cut(s) 213, 306, 441
HpyCH4V TGCA 2 cut(s) 258, 292
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 4 cut(s) 30, 105, 632, 639
Hsp92I GRCGYC 1 cut(s) 418
Hsp92II CATG 3 cut(s) 258, 349, 406
HspAI GCGC 2 cut(s) 94, 418
KasI GGCGCC 1 cut(s) 417
Kzo9I GATC 4 cut(s) 73, 262, 518, 536
LmnI GCTCC 4 cut(s) 26, 109, 190, 439
LpnPI CCDG 9 cut(s) 47, 70, 179, 320, 368, 400, 433, 460, 537
MabI ACCWGGT 1 cut(s) 446
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 1 cut(s) 300
MalI GATC 4 cut(s) 75, 264, 520, 538
MboI GATC 4 cut(s) 73, 262, 518, 536
MboII GAAGA 7 cut(s) 171, 307, 484, 517, 525, 528, 611
MhlI GDGCHC 2 cut(s) 31, 633
MluCI AATT 5 cut(s) 175, 205, 224, 587, 619
Mly113I GGCGCC 1 cut(s) 418
MnlI CCTC 9 cut(s) 80, 87, 100, 161, 176, 214, 424, 552, 586
Mph1103I ATGCAT 1 cut(s) 260
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 1 cut(s) 214
MspR9I CCNGG 1 cut(s) 448
MvaI CCWGG 1 cut(s) 448
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 355
NarI GGCGCC 1 cut(s) 418
NdeII GATC 4 cut(s) 73, 262, 518, 536
NlaIII CATG 3 cut(s) 258, 349, 406
NlaIV GGNNCC 4 cut(s) 149, 400, 419, 435
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 2 cut(s) 258, 349
OliI CACNNNNGTG 1 cut(s) 214
Ple19I CGATCG 1 cut(s) 539
PluTI GGCGCC 1 cut(s) 421
Psp124BI GAGCTC 2 cut(s) 31, 633
Psp6I CCWGG 1 cut(s) 446
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 1 cut(s) 446
PspN4I GGNNCC 4 cut(s) 149, 400, 419, 435
PspPI GGNCC 1 cut(s) 47
PvuI CGATCG 1 cut(s) 539
RsaI GTAC 2 cut(s) 7, 466
RsaNI GTAC 2 cut(s) 6, 465
RseI CAYNNNNRTG 1 cut(s) 214
SacI GAGCTC 2 cut(s) 31, 633
SaqAI TTAA 1 cut(s) 318
Sau3AI GATC 4 cut(s) 73, 262, 518, 536
Sau96I GGNCC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 2 cut(s) 31, 633
SexAI ACCWGGT 1 cut(s) 446
SfcI CTRYAG 1 cut(s) 453
SfoI GGCGCC 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 214
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 5 cut(s) 175, 205, 224, 587, 619
SspDI GGCGCC 1 cut(s) 417
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 2 cut(s) 31, 633
StyD4I CCNGG 1 cut(s) 446
TaaI ACNGT 3 cut(s) 213, 306, 441
TaqI TCGA 2 cut(s) 72, 539
TasI AATT 5 cut(s) 175, 205, 224, 587, 619
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TspDTI ATGAA 2 cut(s) 595, 600
TspGWI ACGGA 1 cut(s) 39
XapI RAATTY 1 cut(s) 205
XceI RCATGY 2 cut(s) 258, 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 444
XspI CTAG 1 cut(s) 350
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.