FvH4_2g14640

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005824
Physical Location & Seq
Forward (+)
12851878 .. 12858075
6198 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14640.t2

Sequence Viewer

Length: 3057 bp
ATGCGCCTCAGGCTTCCCTATAAGACTTTACACTCCTTCAGCCAACACCGCAGCCTCTCATCCGAATTGATTCTTCCCTATGGAACCCCCGAGCCGCTTCACACCCATCTCTTCCAAATTTGTCGAGAACAATGCAGGCTTATCAAGACCCACAAAGTGTTCGGTGAAATGTCTGAAAGGGAGCTGGCTCAAGCTTCAAGAACCTGCAAGGCTATCCATGCCCAGGGTTTGAAATTTGAATTTGGGTCGAAAGGGTTGCTAGGAAATGGCATTGTGGGTTTTTATGCCAAGTGTGGTAACCTGGGCTATGCCGAGAAGGCATTTAATTGCCTTGAAAATAAGGATGTCTTTGCTTGGAACTCTGTCTTATCGATGTATTCAAACAAAGGATTGCTTGATCAGGTTCTGAACTCATTTCAGTCAATGTGGAATTGCAAGGTTTTGCCGAATGAGTTCACATTTGCAATGGTCTTGTCTGCTTGTACAAGATTGGTGAATATAGAGTATGGTAGACAAGTTCATTGCAGTGTTATAAAGTTGGGGCTTGAGTTGATATCATTTTGTCAAGGTGCACTCATTGATATGTATGCCAAGTGCAATTGCATAAGTGATGCTCAGCAGATATTTGAAAGTGCAATTGAGTTGGATACTGTCTCTTGGACAACAATGATTTCAGGGTATGTTCAAGTTGGGCTGCTTGAGGAAGCACTTAAAGTGTTTAAGGAGATGCAGAGAGTTGGCGGTTTTGTGGACCAGGTGGCCTATGTGACTGTCATAAATGCGTGTGTTGGTCTAGGAAGGCTAGATGATGCATGTGACTTGTTCTCGCAGATGCCTAATCCAAATGATGTGGCATGGAATGTGATGATTTCTGGACATGCAAAGAGAGGTTTTGAGGTAGAGGCTGTTAACTTTTTCCTGCAAATGAGGAAAGGTGGTGTAAAACCGACAAGATCTACACTGGGAAGTCTTTTAAGTGCAATCTCTAGTTTAGCAGCTCTAGACTATGGGCTGATAGTTCATGCAATTGCAATAAAGCAGGGGTTAGAATCTAATGTTTATGTAGGAAGTTCTTTAATCAATATGTATGCCAAGTGTGAAAAAATTGATGATGCTACAAGAATATTTCATTATCTATCGGAGAAAAATGTTGTCTTGTGGAACACAATGCTTGGGGGTTATGCGCAGAATGGTTATGCCCATGAAGTCATAAGTTTATTTACTAATATGAAGGCATGTGGTCTTCACCCTGATCAGTTTACCTACACCAGCATTCTTAGTGCCTGTTCTTGCTTGCAAAATCTTGAAATGGGTCGCCAATTGCATTCAGTCATCATCAAGAACCAATTTGCATCAAATTTATTTGTCGGAAATGCATTGATTGATATGTATGCTAAATCAGGCAATCTGAAGGAAGCAAGGTACCAATTCGAGCTCATAAGAAATCGAGACAAGGTATCTTGGAATGCAATTATTGTTGGGTATGTGCAGGAAGAGGATGAGGATGAGGCTTTTTGTATGTTCAGAAGAATGATATTGCATGTGATTGTGCCTGATGAAGTATCCTTGGCCAGTATACTAAGTGCTTGTGCAAATGTTCAAGCACTGAAGATGGGATGGCAAGTCCACTGTCTATCGATTAAAATTGGTCTAGAAACAAGCCTTTACTCTGGGAGCTCCCTTATTGATATGTATTCCAAATGTGGGCTCATTTGGGATTCACGAAGAGTTCTTGATCTCTTGCCCCATTGCAGTGTGGTCTCGATGAATGCTCTGATTTCTGGCTTTGTTCATAGAAACTTTGAGGAAGCAATAAACATATTTTGTGAAATGCAGGATATTGGATTAAATCCATCTGAAGTTACTTTTTCAAGTCTTTTAGATGCATGCAGTGGACCTAGTATGTTACCACTGGGAAGACAAATCCACAACATAGTACTGAAGAAAGGCCTTTTGTTTGATGGTGACTTCTTAGGGGTCTCTCTTTTGGGGATGTATATGAACTGCCAAAGCAAAATAGATGCCACCAATCTTTTCTTAGAATTCCATAAGCCAAAAAACAAAGTATTATGGACTGCTATGATTTCGGGATTCAGTCAAAATGATTGTATAGAGGAGGCATTGCAGTTCTATCAAGATATGCGTAGTGATAATGCGCTCCCAGACCAAGCAACTTTCGCAAGTGTTCTTCGAGCATGTGCTGTTATATCTTCTTTACAAAATGGTAGACAGATACATTCCCTCATTTTTCATACTGGTTTTAATTTGGATGAGTTAACATGTAGCGCCCTGGTAGACATGTATGCCAAATGTGGGGATGTGGGAAGCTCCATGCAAGTTTTCCAAGAAATGGGTACTAAGAATGGTGTTATCTCTTGGAACTCAATGATCGTTGGTTTTGCTAAAAATGGGTATGCAGAAGATGCATTGAAGATCTTTGATGAGATGAAGCAATCTCATGTTGAGCCTGACGACGTCACATTTCTTGGTGTTCTCACTGCATGTAGTCATGCAGGGAAGGTAGCCGAAGGTCGTGAGATATTTGATTCTATGGTCAACGATTATTTTATTCAACCCAGGGTTGATCATATTTGCTGCATGGTTGATCTTCTGGGCAGATGGGGTTTGCTTAAAGAAGCAGAAGAGTTTATTGATAGGTTAGATATTATACCAAACTCTATGATTTGGGCCACATTGCTTGGTGCTTGCAAATTACATGGAGACGACATAAGGGGTCGGCGTGCGGCAGAGAAACTCAAGGAGTTAGAACCACAAAGTTCGTCACCATATATGCTACTTTCTAGCATGTGTGCTGAATCTGGAAACTGGAATGAGGCCAACTCTTTGAGGAGGGAAATGAAGGAAAAAGGGGTTATTAAGTTGCCTGGATTTAGTTGGATTTTTGTGGGACAGAGGACAAATTACTTTTCTGCTGGGGACAAGTCTCATCCAAGTGCTGCTGAAATTCATGTAGCTTTAAAGTACTTGACAGCAATAATGAAAGGAGAGGGCTATGTTTTTGATGAAACAGAATCTTTGTTGCATGAAGAAGAGTGA

Protein Analysis

1019

Amino Acids

113.61

Weight (kDa)

5.77

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 114 - 162 2e-07 PPR repeat family
PPR_1 PF12854 213 - 244 9.4e-07 PPR repeat
PPR_2 PF13041 216 - 263 2.6e-11 PPR repeat family
PPR PF01535 218 - 247 6.8e-10 PPR repeat
PPR_2 PF13041 281 - 327 2e-07 PPR repeat family
PPR_2 PF13041 382 - 430 4.4e-13 PPR repeat family
PPR PF01535 385 - 415 8.9e-06 PPR repeat
PPR_2 PF13041 484 - 532 3.6e-06 PPR repeat family
PPR_2 PF13041 585 - 631 9.2e-10 PPR repeat family
PPR_2 PF13041 687 - 734 3.1e-09 PPR repeat family
PPR PF01535 689 - 717 5e-06 PPR repeat
PPR_long PF17177 699 - 843 4.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 789 - 836 2.3e-12 PPR repeat family
PPR PF01535 791 - 819 1.6e-08 PPR repeat
E_motif PF20431 910 - 967 3.6e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
AatII GACGTC 1 cut(s) 2478
Acc16I TGCGCA 1 cut(s) 1185
Acc36I ACCTGC 1 cut(s) 212
Acc65I GGTACC 1 cut(s) 1422
AccB1I GGYRCC 1 cut(s) 1422
AccB7I CCANNNNNTGG 1 cut(s) 2350
AccI GTMKAC 4 cut(s) 511, 1576, 2227, 2295
AciI CCGC 4 cut(s) 49, 95, 741, 2744
AcoI YGGCCR 1 cut(s) 1569
AcsI RAATTY 6 cut(s) 117, 233, 239, 1357, 2042, 2964
AcuI CTGAAG 5 cut(s) 22, 1430, 1628, 1878, 1961
AcyI GRCGYC 1 cut(s) 2475
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 5 cut(s) 484, 1424, 1938, 2356, 2984
AfiI CCNNNNNNNGG 4 cut(s) 223, 1704, 2313, 2350
AflIII ACRYGT 2 cut(s) 2279, 2298
AjnI CCWGG 6 cut(s) 222, 300, 753, 2289, 2576, 2884
AluBI AGCT 7 cut(s) 184, 194, 998, 1435, 1677, 2328, 2975
AluI AGCT 7 cut(s) 184, 194, 998, 1435, 1677, 2328, 2975
Alw21I GWGCWC 3 cut(s) 574, 1437, 1679
Alw26I GTCTC 6 cut(s) 658, 1443, 1765, 1984, 2715, 2949
Alw44I GTGCAC 1 cut(s) 570
AlwNI CAGNNNCTG 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 5 cut(s) 759, 1569, 1948, 2688, 2835
ApaLI GTGCAC 1 cut(s) 570
ApeKI GCWGC 5 cut(s) 51, 694, 995, 2595, 2957
ApoI RAATTY 6 cut(s) 117, 233, 239, 1357, 2042, 2964
Asp700I GAANNNNTTC 2 cut(s) 69, 452
Asp718I GGTACC 1 cut(s) 1422
AspLEI GCGC 4 cut(s) 6, 1186, 2158, 2288
AspS9I GGNCC 3 cut(s) 751, 1895, 2688
AsuHPI GGTGA 5 cut(s) 176, 505, 1238, 1976, 2775
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 751, 1895
AxyI CCTNAGG 1 cut(s) 8
BaeGI GKGCMC 1 cut(s) 574
BalI TGGCCA 1 cut(s) 1571
BanI GGYRCC 1 cut(s) 1422
BanII GRGCYC 3 cut(s) 1437, 1679, 1710
BbsI GAAGAC 2 cut(s) 1235, 1924
Bbv12I GWGCWC 3 cut(s) 574, 1437, 1679
BbvI GCAGC 5 cut(s) 63, 681, 1007, 2582, 2944
BccI CCATC 6 cut(s) 114, 1606, 1611, 1861, 1955, 2613
BcgI CGANNNNNNTGC 4 cut(s) 114, 148, 238, 272
BciT130I CCWGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
BciVI GTATCC 2 cut(s) 640, 1573
BclI TGATCA 3 cut(s) 397, 1252, 2584
BcoDI GTCTC 6 cut(s) 658, 1443, 1765, 1984, 2715, 2949
BfaI CTAG 8 cut(s) 260, 794, 803, 987, 1001, 1652, 1899, 2802
BfoI RGCGCY 1 cut(s) 2289
BfuAI ACCTGC 1 cut(s) 212
BfuI GTATCC 2 cut(s) 640, 1573
BglI GCCNNNNNGGC 1 cut(s) 317
BglII AGATCT 2 cut(s) 953, 2433
BisI GCNGC 7 cut(s) 52, 95, 695, 996, 2596, 2745, 2958
BlpI GCTNAGC 1 cut(s) 615
BlsI GCNGC 7 cut(s) 53, 96, 696, 997, 2597, 2746, 2959
BmcAI AGTACT 2 cut(s) 1938, 2984
Bme1390I CCNGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
Bme18I GGWCC 2 cut(s) 751, 1895
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 751, 1895, 2688
BmiI GGNNCC 2 cut(s) 85, 1424
BmrFI CCNGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
BmrI ACTGGG 2 cut(s) 971, 1922
BmsI GCATC 9 cut(s) 601, 717, 799, 822, 1102, 1361, 1873, 2011, 2413
BmuI ACTGGG 2 cut(s) 971, 1922
BpiI GAAGAC 2 cut(s) 1235, 1924
BplI GAGNNNNNCTC 2 cut(s) 2825, 2857
Bpu1102I GCTNAGC 1 cut(s) 615
BpuEI CTTGAG 4 cut(s) 174, 566, 719, 2741
Bsa29I ATCGAT 2 cut(s) 371, 1637
BsaHI GRCGYC 1 cut(s) 2475
BsaI GGTCTC 2 cut(s) 1765, 1984
BsaJI CCNNGG 7 cut(s) 222, 223, 301, 1566, 2289, 2576, 2577
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 1704, 2313, 2350
Bse1I ACTGG 5 cut(s) 966, 1572, 1917, 2260, 2831
Bse21I CCTNAGG 1 cut(s) 8
Bse3DI GCAATG 5 cut(s) 471, 520, 1747, 2120, 2693
BseBI CCWGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
BseCI ATCGAT 2 cut(s) 371, 1637
BseDI CCNNGG 7 cut(s) 222, 223, 301, 1566, 2289, 2576, 2577
BseGI GGATG 9 cut(s) 59, 349, 1504, 1510, 1622, 1998, 2275, 2323, 2947
BseLI CCNNNNNNNGG 4 cut(s) 223, 1704, 2313, 2350
BseMI GCAATG 5 cut(s) 471, 520, 1747, 2120, 2693
BseMII CTCAG 2 cut(s) 22, 629
BseNI ACTGG 5 cut(s) 966, 1572, 1917, 2260, 2831
BseRI GAGGAG 2 cut(s) 2129, 2863
BseSI GKGCMC 1 cut(s) 574
BseXI GCAGC 5 cut(s) 63, 681, 1007, 2582, 2944
BseYI CCCAGC 1 cut(s) 2933
BsgI GTGCAG 1 cut(s) 1508
BshFI GGCC 5 cut(s) 761, 1571, 1950, 2690, 2837
BshNI GGYRCC 1 cut(s) 1422
BshVI ATCGAT 2 cut(s) 371, 1637
BsiHKAI GWGCWC 3 cut(s) 574, 1437, 1679
BsiHKCI CYCGRG 1 cut(s) 89
BslFI GGGAC 2 cut(s) 2922, 2951
BslI CCNNNNNNNGG 4 cut(s) 223, 1704, 2313, 2350
BsmAI GTCTC 6 cut(s) 658, 1443, 1765, 1984, 2715, 2949
BsmBI CGTCTC 1 cut(s) 2715
BsmFI GGGAC 2 cut(s) 2922, 2951
BsmI GAATGC 4 cut(s) 1272, 1324, 1471, 1774
BsnI GGCC 5 cut(s) 761, 1571, 1950, 2690, 2837
Bso31I GGTCTC 2 cut(s) 1765, 1984
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 4 cut(s) 574, 1437, 1679, 1710
Bsp1407I TGTACA 1 cut(s) 482
Bsp143I GATC 8 cut(s) 397, 953, 1252, 1735, 2388, 2433, 2584, 2605
Bsp1720I GCTNAGC 1 cut(s) 615
BspACI CCGC 4 cut(s) 49, 95, 741, 2744
BspANI GGCC 5 cut(s) 761, 1571, 1950, 2690, 2837
BspCNI CTCAG 2 cut(s) 21, 628
BspDI ATCGAT 2 cut(s) 371, 1637
BspLI GGNNCC 2 cut(s) 85, 1424
BspMI ACCTGC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 1422
BspTNI GGTCTC 2 cut(s) 1765, 1984
BsrDI GCAATG 5 cut(s) 471, 520, 1747, 2120, 2693
BsrGI TGTACA 1 cut(s) 482
BsrI ACTGG 5 cut(s) 966, 1572, 1917, 2260, 2831
BssECI CCNNGG 7 cut(s) 222, 223, 301, 1566, 2289, 2576, 2577
BssMI GATC 8 cut(s) 397, 953, 1252, 1735, 2388, 2433, 2584, 2605
BssNAI GTATAC 1 cut(s) 1577
BssNI GRCGYC 1 cut(s) 2475
BssT1I CCWWGG 1 cut(s) 1566
Bst1107I GTATAC 1 cut(s) 1577
Bst2UI CCWGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
Bst4CI ACNGT 3 cut(s) 652, 772, 1631
Bst6I CTCTTC 5 cut(s) 116, 1488, 1720, 2637, 3045
BstACI GRCGYC 1 cut(s) 2475
BstAPI GCANNNNNTGC 1 cut(s) 2423
BstAUI TGTACA 1 cut(s) 482
BstC8I GCNNGC 6 cut(s) 137, 186, 1295, 1888, 2707, 2742
BstDEI CTNAG 7 cut(s) 8, 615, 1277, 1580, 1972, 2038, 2358
BstEII GGTNACC 1 cut(s) 296
BstF5I GGATG 9 cut(s) 59, 349, 1504, 1510, 1622, 1998, 2275, 2323, 2947
BstH2I RGCGCY 1 cut(s) 2289
BstHHI GCGC 4 cut(s) 6, 1186, 2158, 2288
BstKTI GATC 8 cut(s) 400, 956, 1255, 1738, 2391, 2436, 2587, 2608
BstMAI GTCTC 6 cut(s) 658, 1443, 1765, 1984, 2715, 2949
BstMBI GATC 8 cut(s) 397, 953, 1252, 1735, 2388, 2433, 2584, 2605
BstMWI GCNNNNNNNGC 6 cut(s) 10, 48, 218, 317, 2177, 2423
BstNI CCWGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
BstPI GGTNACC 1 cut(s) 296
BstSCI CCNGG 6 cut(s) 222, 300, 753, 2289, 2576, 2884
BstSLI GKGCMC 1 cut(s) 574
BstV1I GCAGC 5 cut(s) 63, 681, 1007, 2582, 2944
BstV2I GAAGAC 2 cut(s) 1235, 1924
BstX2I RGATCY 2 cut(s) 953, 2433
BstYI RGATCY 2 cut(s) 953, 2433
BstZ17I GTATAC 1 cut(s) 1577
Bsu15I ATCGAT 2 cut(s) 371, 1637
Bsu36I CCTNAGG 1 cut(s) 8
BsuI GTATCC 2 cut(s) 640, 1573
BsuRI GGCC 5 cut(s) 761, 1571, 1950, 2690, 2837
BsuTUI ATCGAT 2 cut(s) 371, 1637
BtsCI GGATG 9 cut(s) 59, 349, 1504, 1510, 1622, 1998, 2275, 2323, 2947
BtsI GCAGTG 4 cut(s) 532, 1759, 1897, 2496
BtsIMutI CAGTG 8 cut(s) 532, 959, 1604, 1627, 1759, 1897, 1910, 2496
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 6 cut(s) 137, 186, 1295, 1888, 2707, 2742
CaiI CAGNNNCTG 1 cut(s) 406
CfoI GCGC 4 cut(s) 6, 1186, 2158, 2288
Cfr13I GGNCC 3 cut(s) 751, 1895, 2688
ClaI ATCGAT 2 cut(s) 371, 1637
CsiI ACCWGGT 1 cut(s) 753
Csp6I GTAC 5 cut(s) 483, 1423, 1937, 2355, 2983
CspCI CAANNNNNGTGG 4 cut(s) 831, 866, 2680, 2715
CviQI GTAC 5 cut(s) 483, 1423, 1937, 2355, 2983
DdeI CTNAG 7 cut(s) 8, 615, 1277, 1580, 1972, 2038, 2358
DpnI GATC 8 cut(s) 399, 955, 1254, 1737, 2390, 2435, 2586, 2607
DpnII GATC 8 cut(s) 397, 953, 1252, 1735, 2388, 2433, 2584, 2605
DraI TTTAAA 1 cut(s) 2979
DraIII CACNNNGTG 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 1569
Eam1104I CTCTTC 5 cut(s) 116, 1488, 1720, 2637, 3045
EarI CTCTTC 5 cut(s) 116, 1488, 1720, 2637, 3045
Ecl136II GAGCTC 2 cut(s) 1435, 1677
Eco130I CCWWGG 1 cut(s) 1566
Eco147I AGGCCT 1 cut(s) 1950
Eco24I GRGCYC 3 cut(s) 1437, 1679, 1710
Eco31I GGTCTC 2 cut(s) 1765, 1984
Eco32I GATATC 1 cut(s) 555
Eco47I GGWCC 2 cut(s) 751, 1895
Eco53kI GAGCTC 2 cut(s) 1435, 1677
Eco57I CTGAAG 5 cut(s) 22, 1430, 1628, 1878, 1961
Eco81I CCTNAGG 1 cut(s) 8
Eco88I CYCGRG 1 cut(s) 89
Eco91I GGTNACC 1 cut(s) 296
EcoICRI GAGCTC 2 cut(s) 1435, 1677
EcoO65I GGTNACC 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 2042
EcoRII CCWGG 6 cut(s) 222, 300, 753, 2289, 2576, 2884
EcoRV GATATC 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 1566
EcoT22I ATGCAT 4 cut(s) 814, 1378, 1888, 2428
EcoT38I GRGCYC 3 cut(s) 1437, 1679, 1710
ErhI CCWWGG 1 cut(s) 1566
Esp3I CGTCTC 1 cut(s) 2715
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 378, 410
FaqI GGGAC 2 cut(s) 2922, 2951
FbaI TGATCA 3 cut(s) 397, 1252, 2584
FblI GTMKAC 4 cut(s) 511, 1576, 2227, 2295
Fnu4HI GCNGC 7 cut(s) 52, 95, 695, 996, 2596, 2745, 2958
FokI GGATG 9 cut(s) 46, 356, 1511, 1517, 1629, 2005, 2282, 2330, 2934
FriOI GRGCYC 3 cut(s) 1437, 1679, 1710
Fsp4HI GCNGC 7 cut(s) 52, 95, 695, 996, 2596, 2745, 2958
FspBI CTAG 8 cut(s) 260, 794, 803, 987, 1001, 1652, 1899, 2802
FspI TGCGCA 1 cut(s) 1185
GlaI GCGC 4 cut(s) 5, 1185, 2157, 2287
GluI GCNGC 7 cut(s) 52, 95, 695, 996, 2596, 2745, 2958
GsaI CCCAGC 1 cut(s) 2937
HaeII RGCGCY 1 cut(s) 2289
HaeIII GGCC 5 cut(s) 761, 1571, 1950, 2690, 2837
HhaI GCGC 4 cut(s) 6, 1186, 2158, 2288
Hin1I GRCGYC 1 cut(s) 2475
Hin6I GCGC 4 cut(s) 4, 1184, 2156, 2286
HinP1I GCGC 4 cut(s) 4, 1184, 2156, 2286
HincII GTYRAC 3 cut(s) 910, 2277, 2557
HindII GTYRAC 3 cut(s) 910, 2277, 2557
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 7 cut(s) 70, 1049, 1718, 2091, 2546, 2816, 3032
HpaI GTTAAC 2 cut(s) 910, 2277
HphI GGTGA 5 cut(s) 176, 505, 1238, 1976, 2775
Hpy188I TCNGA 9 cut(s) 64, 175, 408, 1141, 1370, 1410, 1526, 1776, 1858
Hpy99I CGWCG 1 cut(s) 2477
HpyAV CCTTC 8 cut(s) 46, 310, 792, 1225, 1405, 2512, 2522, 2854
HpyCH4III ACNGT 3 cut(s) 652, 772, 1631
HpyCH4IV ACGT 1 cut(s) 2475
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 48, 218, 317, 2177, 2423
HpyF3I CTNAG 7 cut(s) 8, 615, 1277, 1580, 1972, 2038, 2358
HpySE526I ACGT 1 cut(s) 2475
Hsp92I GRCGYC 1 cut(s) 2475
HspAI GCGC 4 cut(s) 4, 1184, 2156, 2286
KpnI GGTACC 1 cut(s) 1426
Ksp22I TGATCA 3 cut(s) 397, 1252, 2584
KspAI GTTAAC 2 cut(s) 910, 2277
Kzo9I GATC 8 cut(s) 397, 953, 1252, 1735, 2388, 2433, 2584, 2605
LmnI GCTCC 5 cut(s) 181, 1674, 1682, 2163, 2333
Lsp1109I GCAGC 5 cut(s) 63, 681, 1007, 2582, 2944
LweI GCATC 9 cut(s) 601, 717, 799, 822, 1102, 1361, 1873, 2011, 2413
MabI ACCWGGT 1 cut(s) 753
MaeI CTAG 8 cut(s) 260, 794, 803, 987, 1001, 1652, 1899, 2802
MaeII ACGT 1 cut(s) 2475
MaeIII GTNAC 8 cut(s) 296, 766, 815, 1861, 1905, 1964, 2476, 2781
MalI GATC 8 cut(s) 399, 955, 1254, 1737, 2390, 2435, 2586, 2607
MboI GATC 8 cut(s) 397, 953, 1252, 1735, 2388, 2433, 2584, 2605
MfeI CAATTG 4 cut(s) 598, 636, 1026, 1319
MflI RGATCY 2 cut(s) 953, 2433
MhlI GDGCHC 4 cut(s) 574, 1437, 1679, 1710
MlsI TGGCCA 1 cut(s) 1571
MluNI TGGCCA 1 cut(s) 1571
MmeI TCCRAC 3 cut(s) 624, 1348, 2876
Mox20I TGGCCA 1 cut(s) 1571
Mph1103I ATGCAT 4 cut(s) 814, 1378, 1888, 2428
MroXI GAANNNNTTC 2 cut(s) 69, 452
MscI TGGCCA 1 cut(s) 1571
MslI CAYNNNNRTG 4 cut(s) 525, 581, 1752, 2952
Msp20I TGGCCA 1 cut(s) 1571
MspR9I CCNGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
MunI CAATTG 4 cut(s) 598, 636, 1026, 1319
Mva1269I GAATGC 4 cut(s) 1272, 1324, 1471, 1774
MvaI CCWGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
MwoI GCNNNNNNNGC 6 cut(s) 10, 48, 218, 317, 2177, 2423
NdeII GATC 8 cut(s) 397, 953, 1252, 1735, 2388, 2433, 2584, 2605
NlaIV GGNNCC 2 cut(s) 85, 1424
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 5 cut(s) 766, 815, 1964, 2476, 2781
NsbI TGCGCA 1 cut(s) 1185
NsiI ATGCAT 4 cut(s) 814, 1378, 1888, 2428
PaeI GCATGC 1 cut(s) 1890
PasI CCCWGGG 2 cut(s) 223, 2577
PceI AGGCCT 1 cut(s) 1950
PciI ACATGT 2 cut(s) 2279, 2298
PctI GAATGC 4 cut(s) 1272, 1324, 1471, 1774
PdmI GAANNNNTTC 2 cut(s) 69, 452
PfeI GAWTC 7 cut(s) 70, 1049, 1718, 2091, 2546, 2816, 3032
PflFI GACNNNGTC 1 cut(s) 2474
PflMI CCANNNNNTGG 1 cut(s) 2350
PkrI GCNGC 7 cut(s) 53, 96, 696, 997, 2597, 2746, 2959
PscI ACATGT 2 cut(s) 2279, 2298
PsiI TTATAA 1 cut(s) 533
Psp124BI GAGCTC 2 cut(s) 1437, 1679
Psp6I CCWGG 6 cut(s) 222, 300, 753, 2289, 2576, 2884
PspEI GGTNACC 1 cut(s) 296
PspFI CCCAGC 1 cut(s) 2933
PspGI CCWGG 6 cut(s) 222, 300, 753, 2289, 2576, 2884
PspN4I GGNNCC 2 cut(s) 85, 1424
PspPI GGNCC 3 cut(s) 751, 1895, 2688
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 2 cut(s) 953, 2433
PsyI GACNNNGTC 1 cut(s) 2474
RsaI GTAC 5 cut(s) 484, 1424, 1938, 2356, 2984
RsaNI GTAC 5 cut(s) 483, 1423, 1937, 2355, 2983
RseI CAYNNNNRTG 4 cut(s) 525, 581, 1752, 2952
SacI GAGCTC 2 cut(s) 1437, 1679
SatI GCNGC 7 cut(s) 52, 95, 695, 996, 2596, 2745, 2958
Sau3AI GATC 8 cut(s) 397, 953, 1252, 1735, 2388, 2433, 2584, 2605
Sau96I GGNCC 3 cut(s) 751, 1895, 2688
ScaI AGTACT 2 cut(s) 1938, 2984
ScrFI CCNGG 6 cut(s) 224, 302, 755, 2291, 2578, 2886
SduI GDGCHC 4 cut(s) 574, 1437, 1679, 1710
SexAI ACCWGGT 1 cut(s) 753
SfaNI GCATC 9 cut(s) 601, 717, 799, 822, 1102, 1361, 1873, 2011, 2413
SinI GGWCC 2 cut(s) 751, 1895
SmiMI CAYNNNNRTG 4 cut(s) 525, 581, 1752, 2952
SmlI CTYRAG 4 cut(s) 189, 545, 698, 2756
SmoI CTYRAG 4 cut(s) 189, 545, 698, 2756
SphI GCATGC 1 cut(s) 1890
SseBI AGGCCT 1 cut(s) 1950
SsiI CCGC 4 cut(s) 49, 95, 741, 2744
SspI AATATT 1 cut(s) 1125
SspMI CTAG 8 cut(s) 260, 794, 803, 987, 1001, 1652, 1899, 2802
SstI GAGCTC 2 cut(s) 1437, 1679
StuI AGGCCT 1 cut(s) 1950
StyD4I CCNGG 6 cut(s) 222, 300, 753, 2289, 2576, 2884
StyI CCWWGG 1 cut(s) 1566
TaaI ACNGT 3 cut(s) 652, 772, 1631
TaiI ACGT 1 cut(s) 2478
TaqI TCGA 8 cut(s) 124, 248, 371, 1431, 1447, 1637, 1763, 2191
TatI WGTACW 3 cut(s) 482, 1936, 2982
TauI GCSGC 2 cut(s) 97, 2747
TfiI GAWTC 7 cut(s) 70, 1049, 1718, 2091, 2546, 2816, 3032
TscAI CASTG 8 cut(s) 532, 966, 1611, 1634, 1759, 1897, 1917, 2503
TseFI GTSAC 5 cut(s) 766, 815, 1964, 2476, 2781
TseI GCWGC 5 cut(s) 51, 694, 995, 2595, 2957
Tsp45I GTSAC 5 cut(s) 766, 815, 1964, 2476, 2781
TspRI CASTG 8 cut(s) 532, 966, 1611, 1634, 1759, 1897, 1917, 2503
Tth111I GACNNNGTC 1 cut(s) 2474
Van91I CCANNNNNTGG 1 cut(s) 2350
VneI GTGCAC 1 cut(s) 570
VpaK11BI GGWCC 2 cut(s) 751, 1895
XapI RAATTY 6 cut(s) 117, 233, 239, 1357, 2042, 2964
XbaI TCTAGA 2 cut(s) 1000, 1651
XmiI GTMKAC 4 cut(s) 511, 1576, 2227, 2295
XmnI GAANNNNTTC 2 cut(s) 69, 452
XspI CTAG 8 cut(s) 260, 794, 803, 987, 1001, 1652, 1899, 2802
ZraI GACGTC 1 cut(s) 2476
ZrmI AGTACT 2 cut(s) 1938, 2984
Zsp2I ATGCAT 4 cut(s) 814, 1378, 1888, 2428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.