Rroxscaffold_7G00191600

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005824
Physical Location & Seq
Reverse (-)
32289151 .. 32293543
4393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00191600.1

Sequence Viewer

Length: 3057 bp
ATGCGCCTCAAGCTTCCCTTCAAAACTTTACACTTCTTCAGCCAAAACCGCAGACTCTCATCTGAATCAACTCTCCCCTATGGAACCCCTGAGCCGCTTCCCACCCATCTCTTCCAAATTTGTCGAGAACAATGCAGGCTTATCAAGACCCACAAAGTGTTCGGTGAAATGTCTGAAAGAGATCTGGCTCAATCTTCAAGAACCTGCAAGGCTATCCATGCCCAGGGCTTGAAATTTGAAGTTGGGTCGAAAGGGTTGCTAGGAAATTCTATTGTGGGTTTTTATGCCAAGTGTGGTAACCTGGGCTATGCCGAGAAGGCATTTAATTGCCTTGATAACAAGGATGTGTTTGCTTGGAACTCTGTCTTGTCGATGTATTCAAATAAGGGATTGCTCGAGCAGGTTTTTAACTCATTTGAGTCAATGTGGAAATGCAAGGATTTGCCGAATGAGTTCACGTTTGCAATGGTCTTGTCTGCTTGTACAAGATTGGGGAATCTAGAGTATGGTAGACAAGTTCATTGCAGTGTTATTAAGATGGGGCTCGAGTTGGTATCATACTGTCAAGGTGCACTTATTGATATGTATGCCAAGTGCACTTGTATAAGTGATGCTCGGCAGATATTTGACAGTGCAGTGGAGTTGGATACTGTCGCTTGGACAACAATGATTTCAGGATATGTTCAAGTTGGGCTGCTTGAGGAAGCACTTAAAGTGTTTAAGGAGATGCAAAGAATTGGCGGTGTTCTGGACCAAGTGGCCTATGTGACTGTCATAAATGCATGTGTTGGTCTAGGAAGGCTAGATGATGCATGTGACTTGTTCTTGCAGATGCCCAATCCAAATGATGTGGCATGGAATGTGATGATTTCTGGGCATGCAAAGAGAGGTTATGAGGTAGAAGCTGTTAACTTTTTCCTGGAAATGAGGAAGAGTGGTGTAAAACCGACAAGATCTACACTGGGAAGTCTTTTAAGTGCAATCTCAAGTTTAGCAGCTCTAGACTATGGGCTGATAGTTCATGCTATGGCAATAAAGCAGGGGTTAGACTCTAATGTTTACGTAGGAAGTTCTTTAATCAATATGTATGCCAAGTGTGAAAAAATTGATGCTGCTAAGAGAATATTTCATTATATATCAGAGAAAAATCTTGTCTTGTGGAATGCAATGCTTGGGGGTTATGCACAGAATGGGTATGCCGATGAAGTCATAAAATTGTTTGCTAATATGAAGGCATGTGGTATTCACCCTGATCAGTTTACCTACACCAGCATTCTTAGTGCCTGTTCTTGCTTGCAAAATCTAGAAATGGGTCGCCAATTGCATTCAGTCATCATCAAGAACCAATTTGCATCAAATCTATTCGTCGGAAATGCATTGGTTGATATGTACGCTAAATCAGGGGATCTGAAGGAAGCAAGGAACCAATTTGAGCTCATAAGCAATCGAGACAATGTGTCTTGGAATGCAATTATTGTTGGATATGTGCAGGAAGAGGACGAGGACAAGGCTTTTTGTATGTTCAGAAGAATGATATTGCATAGGATTGTGCCTGATGAAGTATCCTTGGCCAGTATACTAAGTGCTTGTGCAAATGTTCAAGCACTGGACATGGGACAGCAAGTCCACTGTCTCTCGATTAAAATTGGTCTAGAAACAAGCCTTTATGCTGGGAGCTCCCTTATTGATATGTATTCCAAATGTGGGCTAATTAGGGATGCACGAAGTGTTCTTGATTTCTTGCCCCATTGCAGTGTGGTCTCGATGAATGCTCTGATTTCTGGCTTTGTTCATAGAAACTTCGAAGAAGCAGTAAACATATTTTGTGAGATGCAGGATATTGGATTGAACCCATCTGAAGTTACTTTTTCAAGTCTTTTAGATGCATGCAATGGACCTATTATGCTACCACTGGGAAGACAACTCCACAACATAGTACTAAAGAAAGGCCTTTTGTTTGATGGTGACTTCTTAGGTGTCTCTCTTTTGGGAATGTATATGAACTGCCAAAGCAAAATAGATGCAACCAATCTTTTCTTAGAATTCCCAAAGCCAAAAAGCAAAGTATTATGGACCGCTATGATTTCGGGACTCAGTCAAAATGATTGTATAGAGGAGGCATTGCAGTTGTATCGAGAAATGCGTAGTGATAATGCCCTACCAGACCAAGCAACTTTTGCCAGTGTTCTTCGAGCATGTTCTGTTATATCTTCTTTACAAAATGGTAGAGAGATCCATTCTCTCATTTTTCATACTGGTTTTAATTTGGATGAGTTAACATGTAGTGCCCTGGTAGACATGTATGCCAAATGTGGGGATGTGAGAAGCTCTGTGCAAGTTTTCCAAGAAATGGGTACTAAGAATGGTGTTATTTCTTGGAACTCAATGATTGTTGGTTTTGCTAAAAATGGATATGCAGAAGATGCATTTAAGATCTTTGATGAGATGAAGCAATCTCATGTTGAGCCTGACGACGTCACATTTCTTGGTGTTCTCACTGCATGTAGTCATGCAGGGAAGGTGGCTGAAGGTCGTGAGATCTTTGATTCTATGGTCAATTATTATTCTATTCAACCCAGGGTTGATCATATTTGCTGCATGCTTGATCTTCTGGGCAGATGGGGTTTGCTTAAAGAAGCAGAGGAGTTTATTGATAGGCTAGCTTTTGTACCAAATTCTATGATTTGGGCCACACTGCTTGGTGCTTGCAAATTACATGGAGACGACATAAGGGGTTGGCGTGCGGCTCAGAAACTGAAGGAGTTAGAACCACAAAGTTCATCCCCATATGTGCTACTTTCTAGTATGTGTGCTGAATCTGGAAACTGGAATGAGGCTAACTCTTTGAGGAGGGAAATGAAAGAAAAAGGGGTGACTAAGTTGCCTGGATTTAGTTGGATTTCTGTGGGACAGAGGACAAATTACTTTGTTGCTGGGGACAAGTCTCATCCAAGTGCTGCAGAAATTCATGTCGCTTTAAAGTACTTGACAGCAATAATGAAAGGAGAGGGCTATGTTTTTGATGAAACAGATTCTTTGTTGCATGAAGAAGAGTGA

Protein Analysis

1018

Amino Acids

113.31

Weight (kDa)

5.8

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 114 - 162 1.8e-07 PPR repeat family
PPR_1 PF12854 213 - 244 3.8e-06 PPR repeat
PPR_2 PF13041 216 - 262 1.1e-10 PPR repeat family
PPR PF01535 218 - 247 1.1e-09 PPR repeat
PPR_2 PF13041 281 - 327 4.3e-09 PPR repeat family
PPR PF01535 284 - 314 1.5e-06 PPR repeat
PPR_2 PF13041 383 - 430 4.2e-13 PPR repeat family
PPR PF01535 385 - 415 2e-06 PPR repeat
PPR_2 PF13041 484 - 532 4.6e-07 PPR repeat family
PPR_2 PF13041 585 - 630 1.7e-09 PPR repeat family
PPR_2 PF13041 689 - 733 3.5e-09 PPR repeat family
PPR PF01535 689 - 717 4.5e-07 PPR repeat
PPR_long PF17177 699 - 843 2.6e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 789 - 836 8.7e-13 PPR repeat family
PPR PF01535 791 - 819 2e-08 PPR repeat
E_motif PF20431 911 - 967 1.1e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2478
Acc36I ACCTGC 2 cut(s) 212, 391
AccB7I CCANNNNNTGG 1 cut(s) 2350
AccI GTMKAC 3 cut(s) 511, 1576, 2295
AciI CCGC 5 cut(s) 49, 95, 741, 2076, 2744
AclWI GGATC 2 cut(s) 1413, 2227
AcoI YGGCCR 1 cut(s) 1569
AcsI RAATTY 6 cut(s) 117, 233, 265, 2042, 2674, 2964
AcuI CTGAAG 5 cut(s) 22, 1430, 1878, 2547, 2777
AcyI GRCGYC 1 cut(s) 2475
AdeI CACNNNGTG 2 cut(s) 157, 1727
AfaI GTAC 6 cut(s) 484, 1391, 1938, 2356, 2670, 2984
AfiI CCNNNNNNNGG 4 cut(s) 223, 1704, 2313, 2350
AflIII ACRYGT 2 cut(s) 2279, 2298
AhdI GACNNNNNGTC 2 cut(s) 1456, 1622
AjnI CCWGG 6 cut(s) 222, 300, 918, 2289, 2576, 2884
AluBI AGCT 7 cut(s) 13, 905, 998, 1435, 1677, 2328, 2663
AluI AGCT 7 cut(s) 13, 905, 998, 1435, 1677, 2328, 2663
Alw21I GWGCWC 4 cut(s) 574, 599, 1437, 1679
Alw26I GTCTC 6 cut(s) 1443, 1637, 1765, 1984, 2715, 2949
Alw44I GTGCAC 2 cut(s) 570, 595
AlwI GGATC 2 cut(s) 1413, 2227
AlwNI CAGNNNCTG 1 cut(s) 2755
Ama87I CYCGRG 2 cut(s) 395, 545
AoxI GGCC 4 cut(s) 759, 1569, 1948, 2688
ApaLI GTGCAC 2 cut(s) 570, 595
ApeKI GCWGC 5 cut(s) 694, 995, 1112, 2595, 2957
ApoI RAATTY 6 cut(s) 117, 233, 265, 2042, 2674, 2964
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 4 cut(s) 751, 1895, 2073, 2688
AsuHPI GGTGA 4 cut(s) 176, 1238, 1976, 2884
AsuII TTCGAA 1 cut(s) 1803
AsuNHI GCTAGC 1 cut(s) 2659
AvaI CYCGRG 2 cut(s) 395, 545
AvaII GGWCC 3 cut(s) 751, 1895, 2073
BaeGI GKGCMC 3 cut(s) 574, 599, 2290
BalI TGGCCA 1 cut(s) 1571
BanII GRGCYC 3 cut(s) 546, 1437, 1679
BbsI GAAGAC 1 cut(s) 1924
Bbv12I GWGCWC 4 cut(s) 574, 599, 1437, 1679
BbvI GCAGC 5 cut(s) 681, 1007, 1099, 2582, 2944
BccI CCATC 5 cut(s) 114, 532, 1861, 1955, 2613
BcgI CGANNNNNNTGC 6 cut(s) 114, 148, 238, 272, 2114, 2148
BciT130I CCWGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
BciVI GTATCC 2 cut(s) 640, 1573
BclI TGATCA 2 cut(s) 1252, 2584
BcoDI GTCTC 6 cut(s) 1443, 1637, 1765, 1984, 2715, 2949
BfaI CTAG 9 cut(s) 260, 500, 794, 803, 1001, 1304, 1652, 2660, 2802
BfmI CTRYAG 1 cut(s) 2958
BfuAI ACCTGC 2 cut(s) 212, 391
BfuI GTATCC 2 cut(s) 640, 1573
BglI GCCNNNNNGGC 1 cut(s) 317
BglII AGATCT 4 cut(s) 181, 953, 2433, 2538
BisI GCNGC 7 cut(s) 95, 695, 996, 1113, 2596, 2745, 2958
BlsI GCNGC 7 cut(s) 96, 696, 997, 1114, 2597, 2746, 2959
BmcAI AGTACT 2 cut(s) 1938, 2984
Bme1390I CCNGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
Bme18I GGWCC 3 cut(s) 751, 1895, 2073
BmeRI GACNNNNNGTC 2 cut(s) 1456, 1622
BmeT110I CYCGRG 2 cut(s) 395, 545
BmgT120I GGNCC 4 cut(s) 751, 1895, 2073, 2688
BmiI GGNNCC 2 cut(s) 85, 1424
BmrFI CCNGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
BmrI ACTGGG 2 cut(s) 971, 1922
BmtI GCTAGC 1 cut(s) 2663
BmuI ACTGGG 2 cut(s) 971, 1922
BpiI GAAGAC 1 cut(s) 1924
BplI GAGNNNNNCTC 2 cut(s) 2825, 2857
Bpu10I CCTNAGC 1 cut(s) 90
Bpu14I TTCGAA 1 cut(s) 1803
BpuEI CTTGAG 2 cut(s) 719, 970
BsaAI YACGTR 1 cut(s) 1063
BsaHI GRCGYC 1 cut(s) 2475
BsaI GGTCTC 1 cut(s) 1765
BsaJI CCNNGG 7 cut(s) 222, 223, 301, 1566, 2289, 2576, 2577
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 1704, 2313, 2350
Bse1I ACTGG 7 cut(s) 966, 1572, 1611, 1917, 2181, 2260, 2831
Bse3DI GCAATG 6 cut(s) 471, 520, 1173, 1747, 1897, 2120
BseBI CCWGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
BseDI CCNNGG 7 cut(s) 222, 223, 301, 1566, 2289, 2576, 2577
BseGI GGATG 6 cut(s) 349, 1723, 2275, 2323, 2780, 2947
BseLI CCNNNNNNNGG 4 cut(s) 223, 1704, 2313, 2350
BseMI GCAATG 6 cut(s) 471, 520, 1173, 1747, 1897, 2120
BseMII CTCAG 3 cut(s) 81, 2107, 2762
BseNI ACTGG 7 cut(s) 966, 1572, 1611, 1917, 2181, 2260, 2831
BseRI GAGGAG 3 cut(s) 2129, 2657, 2863
BseSI GKGCMC 3 cut(s) 574, 599, 2290
BseXI GCAGC 5 cut(s) 681, 1007, 1099, 2582, 2944
BseYI CCCAGC 2 cut(s) 1670, 2933
BsgI GTGCAG 2 cut(s) 654, 1508
BshFI GGCC 4 cut(s) 761, 1571, 1950, 2690
BsiHKAI GWGCWC 4 cut(s) 574, 599, 1437, 1679
BsiHKCI CYCGRG 2 cut(s) 395, 545
BslFI GGGAC 4 cut(s) 1629, 2103, 2922, 2951
BslI CCNNNNNNNGG 4 cut(s) 223, 1704, 2313, 2350
BsmAI GTCTC 6 cut(s) 1443, 1637, 1765, 1984, 2715, 2949
BsmBI CGTCTC 1 cut(s) 2715
BsmFI GGGAC 4 cut(s) 1629, 2103, 2922, 2951
BsmI GAATGC 5 cut(s) 1168, 1272, 1324, 1471, 1774
BsnI GGCC 4 cut(s) 761, 1571, 1950, 2690
Bso31I GGTCTC 1 cut(s) 1765
BsoBI CYCGRG 2 cut(s) 395, 545
Bsp119I TTCGAA 1 cut(s) 1803
Bsp1286I GDGCHC 6 cut(s) 546, 574, 599, 1437, 1679, 2290
Bsp1407I TGTACA 1 cut(s) 482
Bsp143I GATC 9 cut(s) 181, 953, 1252, 1405, 2232, 2433, 2538, 2584, 2605
BspACI CCGC 5 cut(s) 49, 95, 741, 2076, 2744
BspANI GGCC 4 cut(s) 761, 1571, 1950, 2690
BspCNI CTCAG 3 cut(s) 82, 2106, 2761
BspLI GGNNCC 2 cut(s) 85, 1424
BspMAI CTGCAG 1 cut(s) 2962
BspMI ACCTGC 2 cut(s) 212, 391
BspOI GCTAGC 1 cut(s) 2663
BspPI GGATC 2 cut(s) 1413, 2227
BspT104I TTCGAA 1 cut(s) 1803
BspTNI GGTCTC 1 cut(s) 1765
BsrDI GCAATG 6 cut(s) 471, 520, 1173, 1747, 1897, 2120
BsrGI TGTACA 1 cut(s) 482
BsrI ACTGG 7 cut(s) 966, 1572, 1611, 1917, 2181, 2260, 2831
BssECI CCNNGG 7 cut(s) 222, 223, 301, 1566, 2289, 2576, 2577
BssMI GATC 9 cut(s) 181, 953, 1252, 1405, 2232, 2433, 2538, 2584, 2605
BssNAI GTATAC 1 cut(s) 1577
BssNI GRCGYC 1 cut(s) 2475
BssT1I CCWWGG 1 cut(s) 1566
Bst1107I GTATAC 1 cut(s) 1577
Bst2UI CCWGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
Bst4CI ACNGT 5 cut(s) 563, 632, 652, 772, 1631
Bst6I CTCTTC 4 cut(s) 116, 926, 1488, 3045
BstACI GRCGYC 1 cut(s) 2475
BstAPI GCANNNNNTGC 2 cut(s) 2177, 2423
BstAUI TGTACA 1 cut(s) 482
BstBAI YACGTR 1 cut(s) 1063
BstBI TTCGAA 1 cut(s) 1803
BstC8I GCNNGC 8 cut(s) 137, 879, 1295, 1888, 2600, 2661, 2707, 2742
BstEII GGTNACC 1 cut(s) 296
BstF5I GGATG 6 cut(s) 349, 1723, 2275, 2323, 2780, 2947
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 9 cut(s) 184, 956, 1255, 1408, 2235, 2436, 2541, 2587, 2608
BstMAI GTCTC 6 cut(s) 1443, 1637, 1765, 1984, 2715, 2949
BstMBI GATC 9 cut(s) 181, 953, 1252, 1405, 2232, 2433, 2538, 2584, 2605
BstMWI GCNNNNNNNGC 6 cut(s) 10, 48, 218, 317, 2177, 2423
BstNI CCWGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
BstPI GGTNACC 1 cut(s) 296
BstSCI CCNGG 6 cut(s) 222, 300, 918, 2289, 2576, 2884
BstSFI CTRYAG 1 cut(s) 2958
BstSLI GKGCMC 3 cut(s) 574, 599, 2290
BstSNI TACGTA 1 cut(s) 1063
BstV1I GCAGC 5 cut(s) 681, 1007, 1099, 2582, 2944
BstV2I GAAGAC 1 cut(s) 1924
BstX2I RGATCY 6 cut(s) 181, 953, 1405, 2232, 2433, 2538
BstYI RGATCY 6 cut(s) 181, 953, 1405, 2232, 2433, 2538
BstZ17I GTATAC 1 cut(s) 1577
BsuI GTATCC 2 cut(s) 640, 1573
BsuRI GGCC 4 cut(s) 761, 1571, 1950, 2690
BtsCI GGATG 6 cut(s) 349, 1723, 2275, 2323, 2780, 2947
BtsI GCAGTG 5 cut(s) 532, 642, 1759, 2496, 2693
BveI ACCTGC 2 cut(s) 212, 391
Cac8I GCNNGC 8 cut(s) 137, 879, 1295, 1888, 2600, 2661, 2707, 2742
CaiI CAGNNNCTG 1 cut(s) 2755
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 4 cut(s) 751, 1895, 2073, 2688
Csp6I GTAC 6 cut(s) 483, 1390, 1937, 2355, 2669, 2983
CspCI CAANNNNNGTGG 4 cut(s) 831, 866, 2680, 2715
CviQI GTAC 6 cut(s) 483, 1390, 1937, 2355, 2669, 2983
DpnI GATC 9 cut(s) 183, 955, 1254, 1407, 2234, 2435, 2540, 2586, 2607
DpnII GATC 9 cut(s) 181, 953, 1252, 1405, 2232, 2433, 2538, 2584, 2605
DraI TTTAAA 1 cut(s) 2979
DraIII CACNNNGTG 2 cut(s) 157, 1727
DriI GACNNNNNGTC 2 cut(s) 1456, 1622
EaeI YGGCCR 1 cut(s) 1569
Eam1104I CTCTTC 4 cut(s) 116, 926, 1488, 3045
Eam1105I GACNNNNNGTC 2 cut(s) 1456, 1622
EarI CTCTTC 4 cut(s) 116, 926, 1488, 3045
Ecl136II GAGCTC 2 cut(s) 1435, 1677
Eco105I TACGTA 1 cut(s) 1063
Eco130I CCWWGG 1 cut(s) 1566
Eco147I AGGCCT 1 cut(s) 1950
Eco24I GRGCYC 3 cut(s) 546, 1437, 1679
Eco31I GGTCTC 1 cut(s) 1765
Eco47I GGWCC 3 cut(s) 751, 1895, 2073
Eco53kI GAGCTC 2 cut(s) 1435, 1677
Eco57I CTGAAG 5 cut(s) 22, 1430, 1878, 2547, 2777
Eco88I CYCGRG 2 cut(s) 395, 545
Eco91I GGTNACC 1 cut(s) 296
EcoICRI GAGCTC 2 cut(s) 1435, 1677
EcoO65I GGTNACC 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 2042
EcoRII CCWGG 6 cut(s) 222, 300, 918, 2289, 2576, 2884
EcoT14I CCWWGG 1 cut(s) 1566
EcoT22I ATGCAT 5 cut(s) 784, 814, 1378, 1888, 2428
EcoT38I GRGCYC 3 cut(s) 546, 1437, 1679
ErhI CCWWGG 1 cut(s) 1566
Esp3I CGTCTC 1 cut(s) 2715
FalI AAGNNNNNCTT 3 cut(s) 34, 558, 590
FaqI GGGAC 4 cut(s) 1629, 2103, 2922, 2951
FauNDI CATATG 1 cut(s) 2788
FbaI TGATCA 2 cut(s) 1252, 2584
FblI GTMKAC 3 cut(s) 511, 1576, 2295
Fnu4HI GCNGC 7 cut(s) 95, 695, 996, 1113, 2596, 2745, 2958
FokI GGATG 6 cut(s) 356, 1730, 2282, 2330, 2767, 2934
FriOI GRGCYC 3 cut(s) 546, 1437, 1679
Fsp4HI GCNGC 7 cut(s) 95, 695, 996, 1113, 2596, 2745, 2958
FspBI CTAG 9 cut(s) 260, 500, 794, 803, 1001, 1304, 1652, 2660, 2802
GlaI GCGC 1 cut(s) 5
GluI GCNGC 7 cut(s) 95, 695, 996, 1113, 2596, 2745, 2958
GsaI CCCAGC 2 cut(s) 1674, 2937
HaeIII GGCC 4 cut(s) 761, 1571, 1950, 2690
HhaI GCGC 1 cut(s) 6
Hin1I GRCGYC 1 cut(s) 2475
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 2 cut(s) 910, 2277
HindII GTYRAC 2 cut(s) 910, 2277
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 9 cut(s) 54, 65, 419, 496, 1049, 2091, 2546, 2816, 3032
HpaI GTTAAC 2 cut(s) 910, 2277
HphI GGTGA 4 cut(s) 176, 1238, 1976, 2884
Hpy188I TCNGA 9 cut(s) 64, 175, 1141, 1370, 1410, 1526, 1776, 1858, 2751
Hpy99I CGWCG 2 cut(s) 1370, 2477
HpyAV CCTTC 8 cut(s) 28, 310, 792, 1225, 1405, 2512, 2522, 2752
HpyCH4III ACNGT 5 cut(s) 563, 632, 652, 772, 1631
HpyCH4IV ACGT 3 cut(s) 458, 1062, 2475
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 48, 218, 317, 2177, 2423
HpySE526I ACGT 3 cut(s) 458, 1062, 2475
Hsp92I GRCGYC 1 cut(s) 2475
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 2 cut(s) 1252, 2584
KspAI GTTAAC 2 cut(s) 910, 2277
Kzo9I GATC 9 cut(s) 181, 953, 1252, 1405, 2232, 2433, 2538, 2584, 2605
LmnI GCTCC 2 cut(s) 1674, 1682
Lsp1109I GCAGC 5 cut(s) 681, 1007, 1099, 2582, 2944
MaeI CTAG 9 cut(s) 260, 500, 794, 803, 1001, 1304, 1652, 2660, 2802
MaeII ACGT 3 cut(s) 458, 1062, 2475
MaeIII GTNAC 7 cut(s) 296, 766, 815, 1861, 1964, 2476, 2872
MalI GATC 9 cut(s) 183, 955, 1254, 1407, 2234, 2435, 2540, 2586, 2607
MboI GATC 9 cut(s) 181, 953, 1252, 1405, 2232, 2433, 2538, 2584, 2605
MfeI CAATTG 1 cut(s) 1319
MflI RGATCY 6 cut(s) 181, 953, 1405, 2232, 2433, 2538
MhlI GDGCHC 6 cut(s) 546, 574, 599, 1437, 1679, 2290
MlsI TGGCCA 1 cut(s) 1571
MluNI TGGCCA 1 cut(s) 1571
MlyI GAGTC 4 cut(s) 48, 428, 1043, 2085
MmeI TCCRAC 4 cut(s) 624, 1348, 1459, 2876
Mox20I TGGCCA 1 cut(s) 1571
Mph1103I ATGCAT 5 cut(s) 784, 814, 1378, 1888, 2428
MroXI GAANNNNTTC 1 cut(s) 452
MscI TGGCCA 1 cut(s) 1571
MslI CAYNNNNRTG 3 cut(s) 525, 1752, 2952
Msp20I TGGCCA 1 cut(s) 1571
MspR9I CCNGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
MunI CAATTG 1 cut(s) 1319
Mva1269I GAATGC 5 cut(s) 1168, 1272, 1324, 1471, 1774
MvaI CCWGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
MwoI GCNNNNNNNGC 6 cut(s) 10, 48, 218, 317, 2177, 2423
NdeI CATATG 1 cut(s) 2788
NdeII GATC 9 cut(s) 181, 953, 1252, 1405, 2232, 2433, 2538, 2584, 2605
NheI GCTAGC 1 cut(s) 2659
NlaIV GGNNCC 2 cut(s) 85, 1424
NmeAIII GCCGAG 2 cut(s) 337, 595
NmuCI GTSAC 5 cut(s) 766, 815, 1964, 2476, 2872
NsiI ATGCAT 5 cut(s) 784, 814, 1378, 1888, 2428
NspV TTCGAA 1 cut(s) 1803
PaeI GCATGC 3 cut(s) 881, 1890, 2602
PaeR7I CTCGAG 2 cut(s) 395, 545
PasI CCCWGGG 2 cut(s) 223, 2577
PceI AGGCCT 1 cut(s) 1950
PciI ACATGT 2 cut(s) 2279, 2298
PcsI WCGNNNNNNNCGW 1 cut(s) 2140
PctI GAATGC 5 cut(s) 1168, 1272, 1324, 1471, 1774
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 5 cut(s) 65, 496, 2546, 2816, 3032
PflFI GACNNNGTC 2 cut(s) 2094, 2474
PflMI CCANNNNNTGG 1 cut(s) 2350
PfoI TCCNGGA 1 cut(s) 918
PkrI GCNGC 7 cut(s) 96, 696, 997, 1114, 2597, 2746, 2959
PleI GAGTC 4 cut(s) 48, 427, 1043, 2085
PpsI GAGTC 4 cut(s) 48, 427, 1043, 2085
Ppu21I YACGTR 1 cut(s) 1063
PscI ACATGT 2 cut(s) 2279, 2298
Psp124BI GAGCTC 2 cut(s) 1437, 1679
Psp6I CCWGG 6 cut(s) 222, 300, 918, 2289, 2576, 2884
PspEI GGTNACC 1 cut(s) 296
PspFI CCCAGC 2 cut(s) 1670, 2933
PspGI CCWGG 6 cut(s) 222, 300, 918, 2289, 2576, 2884
PspN4I GGNNCC 2 cut(s) 85, 1424
PspPI GGNCC 4 cut(s) 751, 1895, 2073, 2688
PspXI VCTCGAGB 2 cut(s) 395, 545
PstI CTGCAG 1 cut(s) 2962
PstNI CAGNNNCTG 1 cut(s) 2755
PsuI RGATCY 6 cut(s) 181, 953, 1405, 2232, 2433, 2538
PsyI GACNNNGTC 2 cut(s) 2094, 2474
RsaI GTAC 6 cut(s) 484, 1391, 1938, 2356, 2670, 2984
RsaNI GTAC 6 cut(s) 483, 1390, 1937, 2355, 2669, 2983
RseI CAYNNNNRTG 3 cut(s) 525, 1752, 2952
SacI GAGCTC 2 cut(s) 1437, 1679
SatI GCNGC 7 cut(s) 95, 695, 996, 1113, 2596, 2745, 2958
Sau3AI GATC 9 cut(s) 181, 953, 1252, 1405, 2232, 2433, 2538, 2584, 2605
Sau96I GGNCC 4 cut(s) 751, 1895, 2073, 2688
ScaI AGTACT 2 cut(s) 1938, 2984
SchI GAGTC 4 cut(s) 48, 428, 1043, 2085
ScrFI CCNGG 6 cut(s) 224, 302, 920, 2291, 2578, 2886
SduI GDGCHC 6 cut(s) 546, 574, 599, 1437, 1679, 2290
SfcI CTRYAG 1 cut(s) 2958
Sfr274I CTCGAG 2 cut(s) 395, 545
SfuI TTCGAA 1 cut(s) 1803
SinI GGWCC 3 cut(s) 751, 1895, 2073
SlaI CTCGAG 2 cut(s) 395, 545
SmiMI CAYNNNNRTG 3 cut(s) 525, 1752, 2952
SmlI CTYRAG 5 cut(s) 8, 395, 545, 698, 985
SmoI CTYRAG 5 cut(s) 8, 395, 545, 698, 985
SnaBI TACGTA 1 cut(s) 1063
SphI GCATGC 3 cut(s) 881, 1890, 2602
SseBI AGGCCT 1 cut(s) 1950
SsiI CCGC 5 cut(s) 49, 95, 741, 2076, 2744
SspI AATATT 1 cut(s) 1125
SspMI CTAG 9 cut(s) 260, 500, 794, 803, 1001, 1304, 1652, 2660, 2802
SstI GAGCTC 2 cut(s) 1437, 1679
StuI AGGCCT 1 cut(s) 1950
StyD4I CCNGG 6 cut(s) 222, 300, 918, 2289, 2576, 2884
StyI CCWWGG 1 cut(s) 1566
TaaI ACNGT 5 cut(s) 563, 632, 652, 772, 1631
TaiI ACGT 3 cut(s) 461, 1065, 2478
TatI WGTACW 3 cut(s) 482, 1936, 2982
TauI GCSGC 2 cut(s) 97, 2747
TfiI GAWTC 5 cut(s) 65, 496, 2546, 2816, 3032
TseFI GTSAC 5 cut(s) 766, 815, 1964, 2476, 2872
TseI GCWGC 5 cut(s) 694, 995, 1112, 2595, 2957
Tsp45I GTSAC 5 cut(s) 766, 815, 1964, 2476, 2872
Tth111I GACNNNGTC 2 cut(s) 2094, 2474
Van91I CCANNNNNTGG 1 cut(s) 2350
VneI GTGCAC 2 cut(s) 570, 595
VpaK11BI GGWCC 3 cut(s) 751, 1895, 2073
XapI RAATTY 6 cut(s) 117, 233, 265, 2042, 2674, 2964
XbaI TCTAGA 4 cut(s) 499, 1000, 1303, 1651
XhoI CTCGAG 2 cut(s) 395, 545
XmiI GTMKAC 3 cut(s) 511, 1576, 2295
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 9 cut(s) 260, 500, 794, 803, 1001, 1304, 1652, 2660, 2802
ZraI GACGTC 1 cut(s) 2476
ZrmI AGTACT 2 cut(s) 1938, 2984
Zsp2I ATGCAT 5 cut(s) 784, 814, 1378, 1888, 2428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.