Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0005824

Physical Location & Seq

Genomic Coordinates Reverse (-)

1066445 .. 1068034

Gene Structure 1590 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g00870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGCAATAAAACTGGGGTTAGAGTCTAACGTTTATGTGGGAAGTTCCTTAATCAATATGTATGCCAAGTGTGAAAAGATGGATTTGTCAAAGAAAATATTTGATTATCTACCAGAGAAAAATGTTGTCTTGTGGAATACGATGCTTGGGGGTTATGCCCAAAATGGGTATGCCAGTGAAGTTATAGAATTGTTTACCATTATGAAGGGGTGTGGTCTTTGCCCTGATGAATTTACCTACACTAGCATTCTTAGCGCATGCTCTAGCTTGGAATATCTAAACATGGGTTGTCAGTTGCATTCACATATCATCAAGAATAAACTTGTATCAAATTTATACATGGGAAATGCATTGGTTTATATGTATGCTAAATCAGGGGATCTGAAGGAAGCACGGAAACAATTTGAGCTCATAAGAAATCGAGACAATATATCATGGAATGCAATTATTGTTGGATACGTACAGGAAGAAGATGAAAACGAGGCTTTTAATATGTTCCGAAGAATGAATTTGCATGGAATTGTGCCTGATGAAGTCTCCTTGGCAAGTATACTAAGTGCGTGTGCAAATTTTCAAGCACTAAAGATGGGACAACAAGTCCACGGTCTCTCAGTTAAAAATGGGCTGGAAACAAGCCTCTATTCTGGGAGCTCCCTTATTGACATGTATTCTAGATGTGGGGTCATCGGCAATGCATGTAAAGCTCTCTATTATATGCCCCACTGGAGCGTGGTATCTGTGAATGCTCTAATTGCTGGTTTTGGCTATAGTAACTTGGCAGAAGCTGTTAATTTGCTTCATGAGATGCAGGAGATAGGATTGAACCCAACTGAAATTACTTTTTCAGTCCTTCTAGATGCATGTAGTGGACCTCTCATGTTAACTCTGGGAAGGCAAATCCACTGCATAGTACTAAAGAAAGGCCTTTTGTGTGATGTTAACTTTTTGGGTGTCTCTCTCTTAGGTATGTACATGAATTCCGAAAGCAAAGTAGATGCACCAGTTCTTTTCTCGGAGTTCCCAAAGCCAAAAAGCAAAGTATTATGGACTGCTATGATCTCAGCACTCAGTAAAAATGATTGCAGTGAGGAGGCGCTACAGTTGTATCAAGAGATGTGTAGCCATAATGCTCTACCCGACCAAGCAACATTTGCTAGTGTTCTTCGAGCATGTGCTGTCATTTCTTCTTTGAACAATGGTAGAGAGATCCATTCCCTCATTTTTCATACCGGTTTTGATCTGGACAAGTTAACTTGTAGTGCCCTCGTAGACATGTATGCTAAATGTGGGGATACGGGTAGTTCTGTGCATGTTTTTAAAGACATGGGTACTAAAAATAGTGTCATTTCTTGGAACTCAATGATAGTTGGGTTTGCTAAAAATGGTTATGCAGAAGATGCATTGAAGATCTTTGATGGGATGAGACACTTTCATGTTTTTCCTGATGATGTCACATTCCTCGGTGTTCTCACTGCATGTAGTCATGCAGGGAAGGTAGCTGAAGGCCAACAAATGTATGATTCTATGGTTTATAAGTATAGTATCCAACCCAGGGTCGATCATGTTGCCTGCATGGGATCGATCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

530

Amino Acids

58.44

Weight (kDa)

5.85

Isoelectric Point (pI)

40.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	40 - 88	1.5e-12	PPR repeat family
PPR	PF01535	43 - 73	3.4e-06	PPR repeat
PPR_2	PF13041	142 - 190	3e-08	PPR repeat family
PPR_2	PF13041	243 - 289	1.9e-07	PPR repeat family
PPR_2	PF13041	346 - 392	1.9e-09	PPR repeat family
PPR	PF01535	347 - 375	1.2e-06	PPR repeat
PPR_2	PF13041	447 - 494	3.5e-09	PPR repeat family
PPR	PF01535	449 - 476	7.6e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005824)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09040 AT3G09040 AT3G09040 AT3G09040
fragaria_vesca	FvH4_2g14640 FvH4_2g14640
malus_domestica	MD05G1015800.v1.1 MD10G1015600.v1.1
prunus_persica	Prupe.8G019100_v2.0.a1
pyrus_communis	pycom05g00870 pycom10g01020
rosa_chinensis	RchiOBHm_Chr6g0277121
rosa_laevigata	RLG00000013330
rosa_multiflora	Rmu_sc0003539.1_g000006 Rmu_sc0006244.1_g000049
rosa_roxburghii	Rroxscaffold_7G00191600
rosa_rugosa	Rorug06G0106300 Rorug06G0106400 Rorug06G0106500 Rorug06G0106500
rosa_samantha	Rh6AG216500 Rh6AG216800 Rh6AG216900 Rh6BG221100 Rh6CG223800 Rh6DG213700
rosa_wichuraiana	Rw6G018890 Rw6G018920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1533
AccI	GTMKAC	2 cut(s)	550, 1269
AclI	AACGTT	1 cut(s)	30
AclWI	GGATC	3 cut(s)	387, 1201, 1585
AcsI	RAATTY	5 cut(s)	230, 331, 508, 568, 976
AcuI	CTGAAG	2 cut(s)	404, 1521
AfaI	GTAC	4 cut(s)	462, 912, 971, 1330
AfiI	CCNNNNNNNGG	2 cut(s)	165, 1552
AflIII	ACRYGT	2 cut(s)	663, 1272
AgeI	ACCGGT	1 cut(s)	1229
AgsI	TTSAA	4 cut(s)	575, 823, 1192, 1405
AhdI	GACNNNNNGTC	1 cut(s)	596
AjnI	CCWGG	1 cut(s)	1550
AluBI	AGCT	6 cut(s)	267, 409, 651, 704, 785, 1499
AluI	AGCT	6 cut(s)	267, 409, 651, 704, 785, 1499
Alw21I	GWGCWC	2 cut(s)	411, 653
Alw26I	GTCTC	5 cut(s)	417, 541, 611, 958, 1417
AlwI	GGATC	3 cut(s)	387, 1201, 1585
AlwNI	CAGNNNCTG	1 cut(s)	785
AoxI	GGCC	2 cut(s)	922, 1504
ApoI	RAATTY	5 cut(s)	230, 331, 508, 568, 976
AsiGI	ACCGGT	1 cut(s)	1229
AspLEI	GCGC	2 cut(s)	257, 1096
AspS9I	GGNCC	1 cut(s)	869
AvaII	GGWCC	1 cut(s)	869
BaeGI	GKGCMC	1 cut(s)	1264
BaeI	ACNNNNGTAYC	2 cut(s)	1088, 1121
BanII	GRGCYC	2 cut(s)	411, 653
Bbv12I	GWGCWC	2 cut(s)	411, 653
BccI	CCATC	3 cut(s)	73, 580, 1409
BcgI	CGANNNNNNTGC	2 cut(s)	1547, 1581
BciT130I	CCWGG	1 cut(s)	1552
BciVI	GTATCC	3 cut(s)	449, 1285, 1553
BcoDI	GTCTC	5 cut(s)	417, 541, 611, 958, 1417
BfaI	CTAG	6 cut(s)	243, 264, 672, 854, 1155, 1588
BfmI	CTRYAG	2 cut(s)	766, 1097
BfoI	RGCGCY	1 cut(s)	1097
BfuI	GTATCC	3 cut(s)	449, 1285, 1553
BglII	AGATCT	1 cut(s)	1407
BmcAI	AGTACT	1 cut(s)	912
Bme1390I	CCNGG	1 cut(s)	1552
Bme18I	GGWCC	1 cut(s)	869
BmeRI	GACNNNNNGTC	1 cut(s)	596
BmgT120I	GGNCC	1 cut(s)	869
BmrFI	CCNGG	1 cut(s)	1552
BmrI	ACTGGG	1 cut(s)	23
BmsI	GCATC	5 cut(s)	132, 795, 847, 985, 1387
BmuI	ACTGGG	1 cut(s)	23
BpmI	CTGGAG	1 cut(s)	745
Bsa29I	ATCGAT	1 cut(s)	1580
BsaAI	YACGTR	1 cut(s)	460
BsaI	GGTCTC	1 cut(s)	611
BsaJI	CCNNGG	5 cut(s)	540, 601, 1459, 1550, 1551
BsaWI	WCCGGW	1 cut(s)	1229
Bsc4I	CCNNNNNNNGG	2 cut(s)	165, 1552
Bse118I	RCCGGY	1 cut(s)	1229
Bse1I	ACTGG	4 cut(s)	18, 174, 728, 1001
Bse3DI	GCAATG	1 cut(s)	697
BseBI	CCWGG	1 cut(s)	1552
BseCI	ATCGAT	1 cut(s)	1580
BseDI	CCNNGG	5 cut(s)	540, 601, 1459, 1550, 1551
BseGI	GGATG	1 cut(s)	1425
BseLI	CCNNNNNNNGG	2 cut(s)	165, 1552
BseMI	GCAATG	1 cut(s)	697
BseMII	CTCAG	3 cut(s)	624, 1074, 1081
BseNI	ACTGG	4 cut(s)	18, 174, 728, 1001
BseRI	GAGGAG	1 cut(s)	1103
BseSI	GKGCMC	1 cut(s)	1264
BshFI	GGCC	2 cut(s)	924, 1506
BshTI	ACCGGT	1 cut(s)	1229
BshVI	ATCGAT	1 cut(s)	1580
BsiHKAI	GWGCWC	2 cut(s)	411, 653
BsiSI	CCGG	1 cut(s)	1230
BslFI	GGGAC	1 cut(s)	603
BslI	CCNNNNNNNGG	2 cut(s)	165, 1552
BsmAI	GTCTC	5 cut(s)	417, 541, 611, 958, 1417
BsmFI	GGGAC	1 cut(s)	603
BsmI	GAATGC	4 cut(s)	246, 298, 445, 748
BsnI	GGCC	2 cut(s)	924, 1506
Bso31I	GGTCTC	1 cut(s)	611
Bsp1286I	GDGCHC	3 cut(s)	411, 653, 1264
Bsp1407I	TGTACA	1 cut(s)	969
Bsp143I	GATC	8 cut(s)	379, 1056, 1206, 1237, 1407, 1558, 1577, 1581
BspANI	GGCC	2 cut(s)	924, 1506
BspCNI	CTCAG	3 cut(s)	623, 1073, 1080
BspDI	ATCGAT	1 cut(s)	1580
BspHI	TCATGA	1 cut(s)	799
BspPI	GGATC	3 cut(s)	387, 1201, 1585
BspTNI	GGTCTC	1 cut(s)	611
BsrDI	GCAATG	1 cut(s)	697
BsrFI	RCCGGY	1 cut(s)	1229
BsrGI	TGTACA	1 cut(s)	969
BsrI	ACTGG	4 cut(s)	18, 174, 728, 1001
BssAI	RCCGGY	1 cut(s)	1229
BssECI	CCNNGG	5 cut(s)	540, 601, 1459, 1550, 1551
BssMI	GATC	8 cut(s)	379, 1056, 1206, 1237, 1407, 1558, 1577, 1581
BssNAI	GTATAC	1 cut(s)	551
BssT1I	CCWWGG	1 cut(s)	540
Bst1107I	GTATAC	1 cut(s)	551
Bst2UI	CCWGG	1 cut(s)	1552
Bst4CI	ACNGT	2 cut(s)	605, 1101
BstAPI	GCANNNNNTGC	2 cut(s)	1151, 1397
BstAUI	TGTACA	1 cut(s)	969
BstBAI	YACGTR	1 cut(s)	460
BstC8I	GCNNGC	2 cut(s)	259, 1570
BstDEI	CTNAG	6 cut(s)	251, 554, 610, 961, 1060, 1067
BstDSI	CCRYGG	1 cut(s)	601
BstF5I	GGATG	1 cut(s)	1425
BstH2I	RGCGCY	1 cut(s)	1097
BstHHI	GCGC	2 cut(s)	257, 1096
BstKTI	GATC	8 cut(s)	382, 1059, 1209, 1240, 1410, 1561, 1580, 1584
BstMAI	GTCTC	5 cut(s)	417, 541, 611, 958, 1417
BstMBI	GATC	8 cut(s)	379, 1056, 1206, 1237, 1407, 1558, 1577, 1581
BstMWI	GCNNNNNNNGC	5 cut(s)	252, 701, 752, 1151, 1397
BstNI	CCWGG	1 cut(s)	1552
BstNSI	RCATGY	8 cut(s)	261, 667, 699, 864, 1173, 1276, 1313, 1479
BstSCI	CCNGG	1 cut(s)	1550
BstSFI	CTRYAG	2 cut(s)	766, 1097
BstSLI	GKGCMC	1 cut(s)	1264
BstSNI	TACGTA	1 cut(s)	460
BstX2I	RGATCY	3 cut(s)	379, 1206, 1407
BstYI	RGATCY	3 cut(s)	379, 1206, 1407
BstZ17I	GTATAC	1 cut(s)	551
Bsu15I	ATCGAT	1 cut(s)	1580
BsuI	GTATCC	3 cut(s)	449, 1285, 1553
BsuRI	GGCC	2 cut(s)	924, 1506
BsuTUI	ATCGAT	1 cut(s)	1580
BtgI	CCRYGG	1 cut(s)	601
BtsCI	GGATG	1 cut(s)	1425
BtsI	GCAGTG	3 cut(s)	901, 1090, 1470
BtsIMutI	CAGTG	5 cut(s)	181, 721, 901, 1090, 1470
Cac8I	GCNNGC	2 cut(s)	259, 1570
CaiI	CAGNNNCTG	1 cut(s)	785
CciI	TCATGA	1 cut(s)	799
CfoI	GCGC	2 cut(s)	257, 1096
Cfr10I	RCCGGY	1 cut(s)	1229
Cfr13I	GGNCC	1 cut(s)	869
ClaI	ATCGAT	1 cut(s)	1580
Csp6I	GTAC	4 cut(s)	461, 911, 970, 1329
CspAI	ACCGGT	1 cut(s)	1229
CviQI	GTAC	4 cut(s)	461, 911, 970, 1329
DdeI	CTNAG	6 cut(s)	251, 554, 610, 961, 1060, 1067
DpnI	GATC	8 cut(s)	381, 1058, 1208, 1239, 1409, 1560, 1579, 1583
DpnII	GATC	8 cut(s)	379, 1056, 1206, 1237, 1407, 1558, 1577, 1581
DraI	TTTAAA	1 cut(s)	1318
DriI	GACNNNNNGTC	1 cut(s)	596
Eam1105I	GACNNNNNGTC	1 cut(s)	596
Ecl136II	GAGCTC	2 cut(s)	409, 651
Eco105I	TACGTA	1 cut(s)	460
Eco130I	CCWWGG	1 cut(s)	540
Eco147I	AGGCCT	1 cut(s)	924
Eco24I	GRGCYC	2 cut(s)	411, 653
Eco31I	GGTCTC	1 cut(s)	611
Eco47I	GGWCC	1 cut(s)	869
Eco53kI	GAGCTC	2 cut(s)	409, 651
Eco57I	CTGAAG	2 cut(s)	404, 1521
EcoICRI	GAGCTC	2 cut(s)	409, 651
EcoRI	GAATTC	1 cut(s)	976
EcoRII	CCWGG	1 cut(s)	1550
EcoT14I	CCWWGG	1 cut(s)	540
EcoT22I	ATGCAT	4 cut(s)	352, 697, 862, 1402
EcoT38I	GRGCYC	2 cut(s)	411, 653
ErhI	CCWWGG	1 cut(s)	540
FaqI	GGGAC	1 cut(s)	603
FblI	GTMKAC	2 cut(s)	550, 1269
FokI	GGATG	1 cut(s)	1432
FriOI	GRGCYC	2 cut(s)	411, 653
FspBI	CTAG	6 cut(s)	243, 264, 672, 854, 1155, 1588
GlaI	GCGC	2 cut(s)	256, 1095
GsuI	CTGGAG	1 cut(s)	745
HaeII	RGCGCY	1 cut(s)	1097
HaeIII	GGCC	2 cut(s)	924, 1506
HapII	CCGG	1 cut(s)	1230
HhaI	GCGC	2 cut(s)	257, 1096
Hin6I	GCGC	2 cut(s)	255, 1094
HinP1I	GCGC	2 cut(s)	255, 1094
HincII	GTYRAC	3 cut(s)	882, 940, 1251
HindII	GTYRAC	3 cut(s)	882, 940, 1251
HinfI	GANTC	2 cut(s)	23, 1520
HpaI	GTTAAC	3 cut(s)	882, 940, 1251
HpaII	CCGG	1 cut(s)	1230
Hpy166II	GTNNAC	8 cut(s)	195, 551, 601, 869, 882, 940, 1251, 1270
Hpy188I	TCNGA	4 cut(s)	384, 500, 982, 1015
Hpy188III	TCNNGA	8 cut(s)	313, 422, 672, 800, 854, 1109, 1241, 1442
Hpy8I	GTNNAC	8 cut(s)	195, 551, 601, 869, 882, 940, 1251, 1270
HpyAV	CCTTC	6 cut(s)	199, 379, 860, 885, 1486, 1496
HpyCH4III	ACNGT	2 cut(s)	605, 1101
HpyCH4IV	ACGT	2 cut(s)	30, 459
HpyF10VI	GCNNNNNNNGC	5 cut(s)	252, 701, 752, 1151, 1397
HpyF3I	CTNAG	6 cut(s)	251, 554, 610, 961, 1060, 1067
HpySE526I	ACGT	2 cut(s)	30, 459
HspAI	GCGC	2 cut(s)	255, 1094
KspAI	GTTAAC	3 cut(s)	882, 940, 1251
Kzo9I	GATC	8 cut(s)	379, 1056, 1206, 1237, 1407, 1558, 1577, 1581
LmnI	GCTCC	3 cut(s)	648, 656, 726
LweI	GCATC	5 cut(s)	132, 795, 847, 985, 1387
MaeI	CTAG	6 cut(s)	243, 264, 672, 854, 1155, 1588
MaeII	ACGT	2 cut(s)	30, 459
MaeIII	GTNAC	2 cut(s)	770, 1450
MalI	GATC	8 cut(s)	381, 1058, 1208, 1239, 1409, 1560, 1579, 1583
MboI	GATC	8 cut(s)	379, 1056, 1206, 1237, 1407, 1558, 1577, 1581
MboII	GAAGA	8 cut(s)	479, 482, 513, 1154, 1176, 1406, 1417, 1576
MflI	RGATCY	3 cut(s)	379, 1206, 1407
MhlI	GDGCHC	3 cut(s)	411, 653, 1264
MlyI	GAGTC	1 cut(s)	32
MmeI	TCCRAC	2 cut(s)	433, 1570
MnlI	CCTC	8 cut(s)	475, 647, 882, 1081, 1084, 1226, 1274, 1469
Mph1103I	ATGCAT	4 cut(s)	352, 697, 862, 1402
MseI	TTAA	8 cut(s)	50, 489, 615, 789, 881, 939, 1250, 1317
MslI	CAYNNNNRTG	1 cut(s)	1431
MspI	CCGG	1 cut(s)	1230
MspR9I	CCNGG	1 cut(s)	1552
Mva1269I	GAATGC	4 cut(s)	246, 298, 445, 748
MvaI	CCWGG	1 cut(s)	1552
MwoI	GCNNNNNNNGC	5 cut(s)	252, 701, 752, 1151, 1397
NdeII	GATC	8 cut(s)	379, 1056, 1206, 1237, 1407, 1558, 1577, 1581
NmuCI	GTSAC	1 cut(s)	1450
NsiI	ATGCAT	4 cut(s)	352, 697, 862, 1402
NspI	RCATGY	8 cut(s)	261, 667, 699, 864, 1173, 1276, 1313, 1479
PaeI	GCATGC	1 cut(s)	261
PagI	TCATGA	1 cut(s)	799
PasI	CCCWGGG	1 cut(s)	1551
PceI	AGGCCT	1 cut(s)	924
PciI	ACATGT	2 cut(s)	663, 1272
PctI	GAATGC	4 cut(s)	246, 298, 445, 748
PfeI	GAWTC	1 cut(s)	1520
PinAI	ACCGGT	1 cut(s)	1229
PleI	GAGTC	1 cut(s)	31
PpsI	GAGTC	1 cut(s)	31
Ppu21I	YACGTR	1 cut(s)	460
PscI	ACATGT	2 cut(s)	663, 1272
PsiI	TTATAA	1 cut(s)	1533
Psp124BI	GAGCTC	2 cut(s)	411, 653
Psp1406I	AACGTT	1 cut(s)	30
Psp6I	CCWGG	1 cut(s)	1550
PspGI	CCWGG	1 cut(s)	1550
PspPI	GGNCC	1 cut(s)	869
PstNI	CAGNNNCTG	1 cut(s)	785
PsuI	RGATCY	3 cut(s)	379, 1206, 1407
RsaI	GTAC	4 cut(s)	462, 912, 971, 1330
RsaNI	GTAC	4 cut(s)	461, 911, 970, 1329
RseI	CAYNNNNRTG	1 cut(s)	1431
SacI	GAGCTC	2 cut(s)	411, 653
SaqAI	TTAA	8 cut(s)	50, 489, 615, 789, 881, 939, 1250, 1317
Sau3AI	GATC	8 cut(s)	379, 1056, 1206, 1237, 1407, 1558, 1577, 1581
Sau96I	GGNCC	1 cut(s)	869
ScaI	AGTACT	1 cut(s)	912
SchI	GAGTC	1 cut(s)	32
ScrFI	CCNGG	1 cut(s)	1552
SduI	GDGCHC	3 cut(s)	411, 653, 1264
SfaNI	GCATC	5 cut(s)	132, 795, 847, 985, 1387
SfcI	CTRYAG	2 cut(s)	766, 1097
SinI	GGWCC	1 cut(s)	869
SmiMI	CAYNNNNRTG	1 cut(s)	1431
SnaBI	TACGTA	1 cut(s)	460
SphI	GCATGC	1 cut(s)	261
SseBI	AGGCCT	1 cut(s)	924
SspI	AATATT	1 cut(s)	99
SspMI	CTAG	6 cut(s)	243, 264, 672, 854, 1155, 1588
SstI	GAGCTC	2 cut(s)	411, 653
StuI	AGGCCT	1 cut(s)	924
StyD4I	CCNGG	1 cut(s)	1550
StyI	CCWWGG	1 cut(s)	540
TaaI	ACNGT	2 cut(s)	605, 1101
TaiI	ACGT	2 cut(s)	33, 462
TaqI	TCGA	4 cut(s)	421, 1165, 1557, 1580
TatI	WGTACW	2 cut(s)	910, 969
TfiI	GAWTC	1 cut(s)	1520
Tru1I	TTAA	8 cut(s)	50, 489, 615, 789, 881, 939, 1250, 1317
Tru9I	TTAA	8 cut(s)	50, 489, 615, 789, 881, 939, 1250, 1317
TscAI	CASTG	5 cut(s)	181, 728, 908, 1090, 1477
TseFI	GTSAC	1 cut(s)	1450
Tsp45I	GTSAC	1 cut(s)	1450
TspDTI	ATGAA	9 cut(s)	218, 243, 489, 521, 546, 788, 989, 1214, 1421
TspGWI	ACGGA	1 cut(s)	409
TspRI	CASTG	5 cut(s)	181, 728, 908, 1090, 1477
VpaK11BI	GGWCC	1 cut(s)	869
XapI	RAATTY	5 cut(s)	230, 331, 508, 568, 976
XbaI	TCTAGA	2 cut(s)	671, 853
XceI	RCATGY	8 cut(s)	261, 667, 699, 864, 1173, 1276, 1313, 1479
XmiI	GTMKAC	2 cut(s)	550, 1269
XspI	CTAG	6 cut(s)	243, 264, 672, 854, 1155, 1588
ZrmI	AGTACT	1 cut(s)	912
Zsp2I	ATGCAT	4 cut(s)	352, 697, 862, 1402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.