MD05G1015800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005824
Physical Location & Seq
Reverse (-)
2856763 .. 2859825
3063 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1015800.v1.1.491

Sequence Viewer

Length: 3063 bp
ATGCGTTTCAGACCTCCTCCCTCCAAAACCTTACACTTCTTCCACCGAAAGCAGAGCCTCTCTACTATTCCGTCCGAGTCGGTCTGCCAAGATGGAGCATCTGAGTCCCATTACACCCCTCTCTTGCAGATATGTCTACAGCAATGCAAGTATATTAAGACCCATAAGGTGTTCGACGAAATGCCTGACAGGCTGCTGGCTCGTGCTTCCAGGACCTGCAAGGCTGTCCACGCCCGCAGCTTGACATTTGGGATTTCATCCAAAGGGCTTCTGGGAAATGCCATTCTGGGTTTTTATGCCAAGTGCGGTAATGTGGATTTTGCCAAGAAGGCATTTGACTGCCTTGAAAACAAGGATGTGTTTGCTTGGAACTCAGTTTTGTCAATGTATTCGAGTAAGGGATTGCTGAAACAGGTTGTTAAGTCATTTGGATCGATGTGGAATGGCAGGGTTATGCCGAACGAGTTCACTTTCGCAATGGTTTTGTCTGGTTGTGCAAGATTGGCTGGTGTTGAATATGGTAGGCAAGTTCATTGTGGTGTTATTAAGACGGGGTTTGAGTCGAGTTCATTTTGTGAAGGTGCACTCATTGATATGTATGCCAAGTGCAATTGTATAAGTGATGCACGGCGGGTATTTGATGGCGTGTTGAAGTTGGATACAGTTGCTTGGACAACAATGATCTCAGGGTATGCTCAAGCTGGATTGCTCGAGGAGGCACTTAAAGTGTTTATGGAGATGCAAAGAGTAGGTGGTTTTCTGGACCAGGTGGGATTCGTGACTGTCATAAATGCATGCGTTGGTCTAGGAAGGCTAGATGACGCATGTGAGTTGTTTTCCCGGATGCCCAGTCCTAATGTTGTAGCATGGAATGTGATGATTTCTGGGCATGCCAAGAGAGGCAATGAGGAGAAAGCTGTTAACTTTTTCTTGAGGATGAGGAAATCTGGCGAAAAACCGACAAGATCCACGCTAGGAAGTGTTTTGAGCGCAATTGCTAGTTTAGCTGCTCTAGACTATGGCTTGCTAGTTCACAATATGGCAGTAAAACTGGGGTTAGATTCTAACGTTTATGTGGGAAGTTCTTTAATCAATATGTATGCCAAGTGTGAAAAGATGGATTCGTCAAAGAAAATATTTGATTATCTACCAGAGAAAAATGTTGTCTTGTGGAATGCGATGCTTGGGGGTTATGCCCAAAATGGGTATGCCAGTGAAGTTATAGAATTGTTTACCATTATGAAGGGGTGTGGTCTTTGCCCTGATGAATTTACCTACACTAGCATTCTTAGCGCATGCTCTAGCTTGGAATATCTAAACATGGGTTGTCAGTTGCATTCACATATCATCAAGAATAAACTTGCATCAAATTTATACGTGGGAAATGCATTGGTTGATATGTATGCTAAATCAGGGGATCTGAAGGAAGCAAGGAAACAATTTGAGCTTATAAGAAATCGAGACAATATATCATGGAATGCAATTATTGTTGGATACGTACAGGAAGAAGATGAAGACGAGGCTTTTAATATGTTCCGAAGAATGAAATTGCATGGAATTGTGCCTGATGAAGTCTCCTTGGCAAGTATACTAAGTGCGTGTGCAAATGTTCAAGCACTAAAGATGGGACAACAAGTCCACGGTCTCTCAGTTAAAAATGGGCTGGAAACAAGCCTCTATTCTGGGAGCTCCCTTATTGACATGTATTCTAAATGTGGGGTCATCGGCAATGCACGTAAAGCTCTCTGTTATATGCCCCACTGGAGCGTGGTCTCTGTGAATGCTCTAATTGCTGGTTTTGGCTATAGCAACTTGGCAGAAGCCATTAATTTGCTTCATGAGATGCAGGAGATAGGATTGAACCCAACTGAAATTACTTTTTCAGTCCTTCTAGATGCATGTAGTGGACCTCTCATGTTAACTCTGGGAAGGCAAATCCACTGCATAGTACTAAAGAAAGGCCTTTTGTGTGATGTTGACTTCTTGGGTGTCTCTCTCCTAGGCATGTACATGAACTCCCAAAGCAAAGTAGATGCAACAATTCTTTTCTCGGAGTTCCCAAAGCCAAAAAGCAAAGTATTATGGACCTCTATGATCTCAGGACTCAGTCAAAATGATTGCAGTGAGGAGGCGCTACAGTTGTATCGAGAGATGCATAGCAATAATGCCCTACCTGACCAAGCAACATTTGCTAGTGTTCTTCGAGCATGTGCTGTCATTTCTTCTTTGAAAAATGGTAGAGAGATCCATTCCCTCATTTTTCATACCGGTTTTGATCTGGACGAGTTAACTTGTAGTGCCCTCGTAGACATGTATGCTAAATGTGGGGATGTGGGTAGTTCTGTGCATGTTTTTAAAGAAATGGGTACTAAAAATAGTGTCATTTCTTGGAACTCAATGATAGTTGGGTTTGCTAAAAATGGTTATGCAGAAGATGCATTGAAAATCTTTGATGAGATGAGACACTTTCATATTTTGCCTGACGATGTCACATTCCTTGGCGTTCTCACTGCATGTAGTCATGCAGGGAAGGTAGCTGAAGGCCGACAAATGTATGATTCTATGGTTAATAAGTATAGTATCCAACCCAGGGTCGATCATGTTGCCTGCATGGTTGATCTTCTAGGCCGGTGGGGATTTCTTAAAGAAGCAGAGGAGTTTGTTGACAGACTAGATTCTGACCCAAATGCTATGATTTGGGCCACATTACTTGGTGCTTGCAGATTACATGGAGATTACATAAGGGGACAGCGTGCGGCTGAGAAACTCATTGAGTTAGAACCACAAAATTCTTCAACATATGTGCTGCTTTCTAATATACATGCTGCATCAGGAAACTGGGATGAGGCTAGCTCTTTGAGGAGAGAAATGAAAGAAAAGAAATTGACAAAGGTTCCTGGTTGTAGTTGGATTGTAGTGGGACAGACAACAAGTTTGTTTGTTGCTGGGGATAAGTCTCATCCAAATGCTGGTGAAATCATTGCGGCTTTAAAGTATTTGACAGCAGTAATGAAAGAAGAGGGCTATGTTGATGAGACAGATTATTTCTTCAATGAAGAATAG

Protein Analysis

1021

Amino Acids

112.69

Weight (kDa)

6.45

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 118 - 167 5.9e-08 PPR repeat family
PPR_1 PF12854 217 - 247 4.7e-07 PPR repeat
PPR_2 PF13041 220 - 266 4.3e-09 PPR repeat family
PPR PF01535 222 - 251 1.6e-09 PPR repeat
PPR_2 PF13041 285 - 332 3.6e-09 PPR repeat family
PPR PF01535 288 - 317 1.1e-06 PPR repeat
PPR_2 PF13041 386 - 434 8.4e-12 PPR repeat family
PPR_2 PF13041 488 - 536 6.4e-09 PPR repeat family
PPR_2 PF13041 589 - 635 3.5e-07 PPR repeat family
PPR_2 PF13041 693 - 738 7e-09 PPR repeat family
PPR PF01535 693 - 721 1.1e-06 PPR repeat
PPR_long PF17177 704 - 853 2.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 793 - 840 2.3e-10 PPR repeat family
PPR PF01535 795 - 822 6.5e-08 PPR repeat
E_motif PF20431 912 - 971 2.6e-19 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1451
Acc36I ACCTGC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 2969
AccI GTMKAC 3 cut(s) 136, 1588, 2307
AciI CCGC 5 cut(s) 235, 306, 631, 2756, 2984
AclI AACGTT 1 cut(s) 1068
AclWI GGATC 4 cut(s) 439, 960, 1425, 2239
AcsI RAATTY 3 cut(s) 1268, 1369, 2788
AcuI CTGAAG 2 cut(s) 1442, 2559
AfaI GTAC 4 cut(s) 1500, 1950, 2009, 2368
AfiI CCNNNNNNNGG 3 cut(s) 1203, 2590, 2969
AflIII ACRYGT 2 cut(s) 1701, 2310
AgeI ACCGGT 1 cut(s) 2267
AgsI TTSAA 9 cut(s) 347, 515, 652, 1613, 1861, 2230, 2443, 2796, 3052
AhdI GACNNNNNGTC 1 cut(s) 1634
AjnI CCWGG 4 cut(s) 209, 765, 2588, 2896
Alw21I GWGCWC 2 cut(s) 586, 1691
Alw26I GTCTC 8 cut(s) 1455, 1579, 1649, 1777, 1996, 2455, 2961, 3029
Alw44I GTGCAC 1 cut(s) 582
AlwI GGATC 4 cut(s) 439, 960, 1425, 2239
AlwNI CAGNNNCTG 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 710
AoxI GGCC 4 cut(s) 1960, 2542, 2626, 2700
ApaLI GTGCAC 1 cut(s) 582
ApeKI GCWGC 5 cut(s) 193, 237, 1007, 2806, 2825
ApoI RAATTY 3 cut(s) 1268, 1369, 2788
ArsI GACNNNNNNTTYG 2 cut(s) 2917, 2949
AseI ATTAAT 1 cut(s) 1827
AsiGI ACCGGT 1 cut(s) 2267
Asp700I GAANNNNTTC 1 cut(s) 464
AspA2I CCTAGG 1 cut(s) 1999
AspLEI GCGC 3 cut(s) 992, 1295, 2134
AspS9I GGNCC 5 cut(s) 213, 763, 1907, 2085, 2700
AsuC2I CCSGG 1 cut(s) 841
AsuHPI GGTGA 1 cut(s) 2984
AsuNHI GCTAGC 1 cut(s) 2849
AvaI CYCGRG 1 cut(s) 710
AvaII GGWCC 4 cut(s) 213, 763, 1907, 2085
AvrII CCTAGG 1 cut(s) 1999
BaeGI GKGCMC 2 cut(s) 586, 2302
BaeI ACNNNNGTAYC 2 cut(s) 2126, 2159
BanII GRGCYC 1 cut(s) 1691
BauI CACGAG 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 1521
Bbv12I GWGCWC 2 cut(s) 586, 1691
BbvI GCAGC 5 cut(s) 180, 249, 994, 2793, 2812
BccI CCATC 4 cut(s) 86, 635, 1111, 1618
BceAI ACGGC 1 cut(s) 644
BcgI CGANNNNNNTGC 2 cut(s) 2585, 2619
BciT130I CCWGG 4 cut(s) 211, 767, 2590, 2898
BciVI GTATCC 3 cut(s) 652, 1487, 2591
BcnI CCSGG 1 cut(s) 841
BcoDI GTCTC 8 cut(s) 1455, 1579, 1649, 1777, 1996, 2455, 2961, 3029
BfmI CTRYAG 3 cut(s) 137, 1804, 2135
BfoI RGCGCY 1 cut(s) 2135
BfuAI ACCTGC 1 cut(s) 224
BfuI GTATCC 3 cut(s) 652, 1487, 2591
BglI GCCNNNNNGGC 2 cut(s) 190, 329
BisI GCNGC 7 cut(s) 194, 238, 1008, 2757, 2807, 2826, 2985
BlnI CCTAGG 1 cut(s) 1999
BlsI GCNGC 7 cut(s) 195, 239, 1009, 2758, 2808, 2827, 2986
BmcAI AGTACT 1 cut(s) 1950
Bme1390I CCNGG 5 cut(s) 211, 767, 841, 2590, 2898
Bme18I GGWCC 4 cut(s) 213, 763, 1907, 2085
BmeRI GACNNNNNGTC 1 cut(s) 1634
BmeT110I CYCGRG 1 cut(s) 710
BmgT120I GGNCC 5 cut(s) 213, 763, 1907, 2085, 2700
BmiI GGNNCC 1 cut(s) 2895
BmrFI CCNGG 5 cut(s) 211, 767, 841, 2590, 2898
BmrI ACTGGG 3 cut(s) 843, 1061, 2848
BmtI GCTAGC 1 cut(s) 2853
BmuI ACTGGG 3 cut(s) 843, 1061, 2848
BpiI GAAGAC 1 cut(s) 1521
BplI GAGNNNNNCTC 2 cut(s) 2837, 2869
BpmI CTGGAG 1 cut(s) 1783
BpuEI CTTGAG 2 cut(s) 681, 952
BpuMI CCSGG 1 cut(s) 841
Bsa29I ATCGAT 1 cut(s) 434
BsaAI YACGTR 3 cut(s) 1378, 1498, 1736
BsaI GGTCTC 2 cut(s) 1649, 1777
BsaJI CCNNGG 6 cut(s) 1578, 1639, 1999, 2497, 2588, 2589
BsaWI WCCGGW 1 cut(s) 2267
BsaXI ACNNNNNCTCC 2 cut(s) 2000, 2030
Bsc4I CCNNNNNNNGG 3 cut(s) 1203, 2590, 2969
Bse118I RCCGGY 2 cut(s) 2267, 2628
Bse1I ACTGG 5 cut(s) 849, 1056, 1212, 1766, 2843
Bse3DI GCAATG 5 cut(s) 149, 483, 910, 1735, 2979
BseBI CCWGG 4 cut(s) 211, 767, 2590, 2898
BseCI ATCGAT 1 cut(s) 434
BseDI CCNNGG 6 cut(s) 1578, 1639, 1999, 2497, 2588, 2589
BseGI GGATG 7 cut(s) 257, 361, 849, 942, 2335, 2848, 2959
BseLI CCNNNNNNNGG 3 cut(s) 1203, 2590, 2969
BseMI GCAATG 5 cut(s) 149, 483, 910, 1735, 2979
BseMII CTCAG 7 cut(s) 93, 387, 699, 1662, 2112, 2119, 2751
BseNI ACTGG 5 cut(s) 849, 1056, 1212, 1766, 2843
BseRI GAGGAG 6 cut(s) 6, 728, 923, 2141, 2669, 2875
BseSI GKGCMC 2 cut(s) 586, 2302
BseXI GCAGC 5 cut(s) 180, 249, 994, 2793, 2812
BseYI CCCAGC 1 cut(s) 2945
BshFI GGCC 4 cut(s) 1962, 2544, 2628, 2702
BshTI ACCGGT 1 cut(s) 2267
BshVI ATCGAT 1 cut(s) 434
BsiHKAI GWGCWC 2 cut(s) 586, 1691
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 3 cut(s) 841, 2268, 2629
BslFI GGGAC 4 cut(s) 91, 1641, 2760, 2934
BslI CCNNNNNNNGG 3 cut(s) 1203, 2590, 2969
BsmAI GTCTC 8 cut(s) 1455, 1579, 1649, 1777, 1996, 2455, 2961, 3029
BsmFI GGGAC 4 cut(s) 91, 1641, 2760, 2934
BsmI GAATGC 5 cut(s) 1180, 1284, 1336, 1483, 1786
BsnI GGCC 4 cut(s) 1962, 2544, 2628, 2702
Bso31I GGTCTC 2 cut(s) 1649, 1777
BsoBI CYCGRG 1 cut(s) 710
Bsp1286I GDGCHC 3 cut(s) 586, 1691, 2302
Bsp1407I TGTACA 1 cut(s) 2007
Bsp143I GATC 9 cut(s) 431, 681, 965, 1417, 2094, 2244, 2275, 2596, 2617
BspACI CCGC 5 cut(s) 235, 306, 631, 2756, 2984
BspANI GGCC 4 cut(s) 1962, 2544, 2628, 2702
BspCNI CTCAG 7 cut(s) 94, 386, 698, 1661, 2111, 2118, 2752
BspDI ATCGAT 1 cut(s) 434
BspHI TCATGA 1 cut(s) 1837
BspLI GGNNCC 1 cut(s) 2895
BspMI ACCTGC 1 cut(s) 224
BspOI GCTAGC 1 cut(s) 2853
BspPI GGATC 4 cut(s) 439, 960, 1425, 2239
BspTNI GGTCTC 2 cut(s) 1649, 1777
BsrDI GCAATG 5 cut(s) 149, 483, 910, 1735, 2979
BsrFI RCCGGY 2 cut(s) 2267, 2628
BsrGI TGTACA 1 cut(s) 2007
BsrI ACTGG 5 cut(s) 849, 1056, 1212, 1766, 2843
BssAI RCCGGY 2 cut(s) 2267, 2628
BssECI CCNNGG 6 cut(s) 1578, 1639, 1999, 2497, 2588, 2589
BssMI GATC 9 cut(s) 431, 681, 965, 1417, 2094, 2244, 2275, 2596, 2617
BssNAI GTATAC 1 cut(s) 1589
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 3 cut(s) 1578, 1999, 2497
Bst1107I GTATAC 1 cut(s) 1589
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 4 cut(s) 211, 767, 2590, 2898
Bst4CI ACNGT 4 cut(s) 664, 784, 1643, 2139
Bst6I CTCTTC 1 cut(s) 3012
BstAPI GCANNNNNTGC 2 cut(s) 2189, 2435
BstAUI TGTACA 1 cut(s) 2007
BstBAI YACGTR 3 cut(s) 1378, 1498, 1736
BstDEI CTNAG 9 cut(s) 102, 373, 685, 1289, 1592, 1648, 2098, 2105, 2760
BstDSI CCRYGG 1 cut(s) 1639
BstF5I GGATG 7 cut(s) 257, 361, 849, 942, 2335, 2848, 2959
BstH2I RGCGCY 1 cut(s) 2135
BstHHI GCGC 3 cut(s) 992, 1295, 2134
BstKTI GATC 9 cut(s) 434, 684, 968, 1420, 2097, 2247, 2278, 2599, 2620
BstMAI GTCTC 8 cut(s) 1455, 1579, 1649, 1777, 1996, 2455, 2961, 3029
BstMBI GATC 9 cut(s) 431, 681, 965, 1417, 2094, 2244, 2275, 2596, 2617
BstNI CCWGG 4 cut(s) 211, 767, 2590, 2898
BstSCI CCNGG 5 cut(s) 209, 765, 839, 2588, 2896
BstSFI CTRYAG 3 cut(s) 137, 1804, 2135
BstSLI GKGCMC 2 cut(s) 586, 2302
BstSNI TACGTA 1 cut(s) 1498
BstV1I GCAGC 5 cut(s) 180, 249, 994, 2793, 2812
BstV2I GAAGAC 1 cut(s) 1521
BstX2I RGATCY 3 cut(s) 965, 1417, 2244
BstYI RGATCY 3 cut(s) 965, 1417, 2244
BstZ17I GTATAC 1 cut(s) 1589
Bsu15I ATCGAT 1 cut(s) 434
BsuI GTATCC 3 cut(s) 652, 1487, 2591
BsuRI GGCC 4 cut(s) 1962, 2544, 2628, 2702
BsuTUI ATCGAT 1 cut(s) 434
BtgI CCRYGG 1 cut(s) 1639
BtgZI GCGATG 1 cut(s) 1193
BtsCI GGATG 7 cut(s) 257, 361, 849, 942, 2335, 2848, 2959
BtsI GCAGTG 3 cut(s) 1939, 2128, 2508
BtsIMutI CAGTG 5 cut(s) 1219, 1759, 1939, 2128, 2508
BveI ACCTGC 1 cut(s) 224
CaiI CAGNNNCTG 1 cut(s) 216
CciI TCATGA 1 cut(s) 1837
CfoI GCGC 3 cut(s) 992, 1295, 2134
Cfr10I RCCGGY 2 cut(s) 2267, 2628
Cfr13I GGNCC 5 cut(s) 213, 763, 1907, 2085, 2700
ClaI ATCGAT 1 cut(s) 434
CseI GACGC 1 cut(s) 830
CsiI ACCWGGT 1 cut(s) 765
Csp6I GTAC 4 cut(s) 1499, 1949, 2008, 2367
CspAI ACCGGT 1 cut(s) 2267
CspCI CAANNNNNGTGG 2 cut(s) 2692, 2727
CviQI GTAC 4 cut(s) 1499, 1949, 2008, 2367
DdeI CTNAG 9 cut(s) 102, 373, 685, 1289, 1592, 1648, 2098, 2105, 2760
DpnI GATC 9 cut(s) 433, 683, 967, 1419, 2096, 2246, 2277, 2598, 2619
DpnII GATC 9 cut(s) 431, 681, 965, 1417, 2094, 2244, 2275, 2596, 2617
DraI TTTAAA 2 cut(s) 2356, 2991
DriI GACNNNNNGTC 1 cut(s) 1634
Eam1104I CTCTTC 1 cut(s) 3012
Eam1105I GACNNNNNGTC 1 cut(s) 1634
EarI CTCTTC 1 cut(s) 3012
Ecl136II GAGCTC 1 cut(s) 1689
Eco105I TACGTA 1 cut(s) 1498
Eco130I CCWWGG 3 cut(s) 1578, 1999, 2497
Eco147I AGGCCT 1 cut(s) 1962
Eco24I GRGCYC 1 cut(s) 1691
Eco31I GGTCTC 2 cut(s) 1649, 1777
Eco47I GGWCC 4 cut(s) 213, 763, 1907, 2085
Eco53kI GAGCTC 1 cut(s) 1689
Eco57I CTGAAG 2 cut(s) 1442, 2559
Eco88I CYCGRG 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 1689
EcoO109I RGGNCCY 1 cut(s) 213
EcoRII CCWGG 4 cut(s) 209, 765, 2588, 2896
EcoT14I CCWWGG 3 cut(s) 1578, 1999, 2497
EcoT22I ATGCAT 5 cut(s) 796, 1390, 1900, 2157, 2440
EcoT38I GRGCYC 1 cut(s) 1691
ErhI CCWWGG 3 cut(s) 1578, 1999, 2497
FaqI GGGAC 4 cut(s) 91, 1641, 2760, 2934
FauI CCCGC 2 cut(s) 242, 624
FauNDI CATATG 1 cut(s) 2800
FblI GTMKAC 3 cut(s) 136, 1588, 2307
Fnu4HI GCNGC 7 cut(s) 194, 238, 1008, 2757, 2807, 2826, 2985
FokI GGATG 7 cut(s) 244, 368, 856, 949, 2342, 2855, 2946
FriOI GRGCYC 1 cut(s) 1691
Fsp4HI GCNGC 7 cut(s) 194, 238, 1008, 2757, 2807, 2826, 2985
GlaI GCGC 3 cut(s) 991, 1294, 2133
GluI GCNGC 7 cut(s) 194, 238, 1008, 2757, 2807, 2826, 2985
GsaI CCCAGC 1 cut(s) 2949
GsuI CTGGAG 1 cut(s) 1783
HaeII RGCGCY 1 cut(s) 2135
HaeIII GGCC 4 cut(s) 1962, 2544, 2628, 2702
HapII CCGG 3 cut(s) 841, 2268, 2629
HgaI GACGC 1 cut(s) 830
HhaI GCGC 3 cut(s) 992, 1295, 2134
Hin6I GCGC 3 cut(s) 990, 1293, 2132
HinP1I GCGC 3 cut(s) 990, 1293, 2132
HincII GTYRAC 5 cut(s) 922, 1920, 1978, 2289, 2665
HindII GTYRAC 5 cut(s) 922, 1920, 1978, 2289, 2665
HinfI GANTC 9 cut(s) 77, 104, 560, 774, 1061, 1121, 2103, 2558, 2675
HpaI GTTAAC 3 cut(s) 922, 1920, 2289
HpaII CCGG 3 cut(s) 841, 2268, 2629
HphI GGTGA 1 cut(s) 2984
Hpy188I TCNGA 7 cut(s) 11, 76, 103, 1422, 1538, 2053, 2680
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 9 cut(s) 322, 572, 804, 1237, 1417, 1898, 1923, 2524, 2534
HpyCH4III ACNGT 4 cut(s) 664, 784, 1643, 2139
HpyCH4IV ACGT 4 cut(s) 1068, 1377, 1497, 1735
HpyF3I CTNAG 9 cut(s) 102, 373, 685, 1289, 1592, 1648, 2098, 2105, 2760
HpySE526I ACGT 4 cut(s) 1068, 1377, 1497, 1735
HspAI GCGC 3 cut(s) 990, 1293, 2132
KspAI GTTAAC 3 cut(s) 922, 1920, 2289
Kzo9I GATC 9 cut(s) 431, 681, 965, 1417, 2094, 2244, 2275, 2596, 2617
LmnI GCTCC 4 cut(s) 95, 1686, 1694, 1764
Lsp1109I GCAGC 5 cut(s) 180, 249, 994, 2793, 2812
MabI ACCWGGT 1 cut(s) 765
MaeII ACGT 4 cut(s) 1068, 1377, 1497, 1735
MaeIII GTNAC 2 cut(s) 778, 2488
MalI GATC 9 cut(s) 433, 683, 967, 1419, 2096, 2246, 2277, 2598, 2619
MboI GATC 9 cut(s) 431, 681, 965, 1417, 2094, 2244, 2275, 2596, 2617
MfeI CAATTG 2 cut(s) 610, 993
MflI RGATCY 3 cut(s) 965, 1417, 2244
MhlI GDGCHC 3 cut(s) 586, 1691, 2302
MlyI GAGTC 4 cut(s) 86, 113, 569, 2097
MmeI TCCRAC 4 cut(s) 636, 1471, 2608, 2888
Mph1103I ATGCAT 5 cut(s) 796, 1390, 1900, 2157, 2440
MroXI GAANNNNTTC 1 cut(s) 464
MslI CAYNNNNRTG 4 cut(s) 537, 593, 2009, 2964
MspI CCGG 3 cut(s) 841, 2268, 2629
MspR9I CCNGG 5 cut(s) 211, 767, 841, 2590, 2898
MunI CAATTG 2 cut(s) 610, 993
Mva1269I GAATGC 5 cut(s) 1180, 1284, 1336, 1483, 1786
MvaI CCWGG 4 cut(s) 211, 767, 2590, 2898
NciI CCSGG 1 cut(s) 841
NdeI CATATG 1 cut(s) 2800
NdeII GATC 9 cut(s) 431, 681, 965, 1417, 2094, 2244, 2275, 2596, 2617
NheI GCTAGC 1 cut(s) 2849
NlaIV GGNNCC 1 cut(s) 2895
NmuCI GTSAC 2 cut(s) 778, 2488
NsiI ATGCAT 5 cut(s) 796, 1390, 1900, 2157, 2440
PaeI GCATGC 3 cut(s) 798, 893, 1299
PaeR7I CTCGAG 1 cut(s) 710
PagI TCATGA 1 cut(s) 1837
PasI CCCWGGG 1 cut(s) 2589
PceI AGGCCT 1 cut(s) 1962
PciI ACATGT 2 cut(s) 1701, 2310
PctI GAATGC 5 cut(s) 1180, 1284, 1336, 1483, 1786
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 5 cut(s) 774, 1061, 1121, 2558, 2675
PflFI GACNNNGTC 2 cut(s) 2106, 2486
PflMI CCANNNNNTGG 1 cut(s) 2969
PfoI TCCNGGA 2 cut(s) 209, 839
PinAI ACCGGT 1 cut(s) 2267
PkrI GCNGC 7 cut(s) 195, 239, 1009, 2758, 2808, 2827, 2986
PleI GAGTC 4 cut(s) 85, 112, 568, 2097
PpsI GAGTC 4 cut(s) 85, 112, 568, 2097
Ppu21I YACGTR 3 cut(s) 1378, 1498, 1736
PpuMI RGGWCCY 1 cut(s) 213
PscI ACATGT 2 cut(s) 1701, 2310
PshBI ATTAAT 1 cut(s) 1827
PsiI TTATAA 1 cut(s) 1451
Psp124BI GAGCTC 1 cut(s) 1691
Psp1406I AACGTT 1 cut(s) 1068
Psp5II RGGWCCY 1 cut(s) 213
Psp6I CCWGG 4 cut(s) 209, 765, 2588, 2896
PspFI CCCAGC 1 cut(s) 2945
PspGI CCWGG 4 cut(s) 209, 765, 2588, 2896
PspN4I GGNNCC 1 cut(s) 2895
PspPI GGNCC 5 cut(s) 213, 763, 1907, 2085, 2700
PspPPI RGGWCCY 1 cut(s) 213
PspXI VCTCGAGB 1 cut(s) 710
PstNI CAGNNNCTG 1 cut(s) 216
PsuI RGATCY 3 cut(s) 965, 1417, 2244
PsyI GACNNNGTC 2 cut(s) 2106, 2486
RsaI GTAC 4 cut(s) 1500, 1950, 2009, 2368
RsaNI GTAC 4 cut(s) 1499, 1949, 2008, 2367
RseI CAYNNNNRTG 4 cut(s) 537, 593, 2009, 2964
SacI GAGCTC 1 cut(s) 1691
SatI GCNGC 7 cut(s) 194, 238, 1008, 2757, 2807, 2826, 2985
Sau3AI GATC 9 cut(s) 431, 681, 965, 1417, 2094, 2244, 2275, 2596, 2617
Sau96I GGNCC 5 cut(s) 213, 763, 1907, 2085, 2700
ScaI AGTACT 1 cut(s) 1950
SchI GAGTC 4 cut(s) 86, 113, 569, 2097
ScrFI CCNGG 5 cut(s) 211, 767, 841, 2590, 2898
SduI GDGCHC 3 cut(s) 586, 1691, 2302
SexAI ACCWGGT 1 cut(s) 765
SfcI CTRYAG 3 cut(s) 137, 1804, 2135
Sfr274I CTCGAG 1 cut(s) 710
SinI GGWCC 4 cut(s) 213, 763, 1907, 2085
SlaI CTCGAG 1 cut(s) 710
SmiMI CAYNNNNRTG 4 cut(s) 537, 593, 2009, 2964
SmlI CTYRAG 3 cut(s) 696, 710, 931
SmoI CTYRAG 3 cut(s) 696, 710, 931
SnaBI TACGTA 1 cut(s) 1498
SphI GCATGC 3 cut(s) 798, 893, 1299
SseBI AGGCCT 1 cut(s) 1962
SsiI CCGC 5 cut(s) 235, 306, 631, 2756, 2984
SspI AATATT 1 cut(s) 1137
SstI GAGCTC 1 cut(s) 1691
StuI AGGCCT 1 cut(s) 1962
StyD4I CCNGG 5 cut(s) 209, 765, 839, 2588, 2896
StyI CCWWGG 3 cut(s) 1578, 1999, 2497
TaaI ACNGT 4 cut(s) 664, 784, 1643, 2139
TaiI ACGT 4 cut(s) 1071, 1380, 1500, 1738
TaqI TCGA 9 cut(s) 174, 392, 434, 563, 711, 1459, 2146, 2203, 2595
TaqII GACCGA 1 cut(s) 70
TatI WGTACW 2 cut(s) 1948, 2007
TauI GCSGC 2 cut(s) 2759, 2987
TfiI GAWTC 5 cut(s) 774, 1061, 1121, 2558, 2675
TscAI CASTG 5 cut(s) 1219, 1766, 1946, 2128, 2515
TseFI GTSAC 2 cut(s) 778, 2488
TseI GCWGC 5 cut(s) 193, 237, 1007, 2806, 2825
Tsp45I GTSAC 2 cut(s) 778, 2488
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 5 cut(s) 1219, 1766, 1946, 2128, 2515
Tth111I GACNNNGTC 2 cut(s) 2106, 2486
Van91I CCANNNNNTGG 1 cut(s) 2969
VneI GTGCAC 1 cut(s) 582
VpaK11BI GGWCC 4 cut(s) 213, 763, 1907, 2085
VspI ATTAAT 1 cut(s) 1827
XapI RAATTY 3 cut(s) 1268, 1369, 2788
XbaI TCTAGA 2 cut(s) 1012, 1891
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 710
XmaJI CCTAGG 1 cut(s) 1999
XmiI GTMKAC 3 cut(s) 136, 1588, 2307
XmnI GAANNNNTTC 1 cut(s) 464
ZrmI AGTACT 1 cut(s) 1950
Zsp2I ATGCAT 5 cut(s) 796, 1390, 1900, 2157, 2440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.