FvH4_2g15600

Flavin containing amine oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012576
Physical Location & Seq
Forward (+)
13700957 .. 13705499
4543 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15600.t1

Sequence Viewer

Length: 1632 bp
ATGTCGTTGGCTTCTTGTTGGAGATTCCCTCTCACACTAGTCCTAGTCCCAACGACACCGCGTTTCAATCACAGACACCCAACTGGGTCATCATCTCGTCGCAGCAGAGCTCCTGCTCAAGCTCAACTAGAGCTTACAGTCCCTGAAGATGGACCAAACGACGACAAGAAAGTGAAGAAGGTTGTCGTCGTTGGCTCTGGATGGGCTGGCCTTGGCGCTGCCCACCACCTCTGCAACCAGCAGGGCATTGATGTTACTGTTCTTGAAACTACTGATACTGGTCTTGATGATGTAGGTGTCCATGGGTATTGGTATCCCTACCGGAACATATTCAGCCTTGTTGATGAGCTTGAGATTAAACCCTTCACTGACTGGACCAAGTTTGCGCAATATTCTGCAGAGGGATTGGAGGTTGAATTTCCGGTATTTCAAGGGCTTCCTCAGTTGCCAACTCCTTTAGGAACCTTGTACTATTCACAGTTTGTTCAGTTGCCATTGGTTGATAGATTAACTGCACTTCCTTTAACAGCTGCAGTAATCGACTTTGACAATACTGATACAGCTTGGAGAAAATATGATTCAATTACTGCAAGGGAACTCTTTAAGCAGTTTGGCTGCTCAGAAAAGCTTTATCAGAAAGTCCTAGCACCATTGCTTCAAGTGGGTCTGTTTGCACCAGCAGAACAATGTAGTGCTGCTGCTACTCTCGGCTTGCTATCCTACATCCTTGCTCACCAGAAAGATTTTGATCTAGTATTGTGCCGTGGAACAACTAGGGAAAAGATCTTTAAGCCTTGGATAGAGTCTATGACAACCAAAGGTTGCAAGTTTGAGAAAGGTGCAAGGGTAACAGACCTTTTGCTTAATGAGGAAACAGGTTGTATTTCTGAAGTAGTTTGTGGCAAAGAGATGTTTAGTGCTGATGCTGTTGTCCTTGCTGTTGGAATCTCCACACTTCAGAAACTTGTTGAAAATAGTGCAGTGTTGTCTACAAGGGAGGAATTTATCAAGGTCTCGAACCTGGATACCATTGATGTACTCTCTGTTAAGCTCTGGCTCGATAGGAAGGTGAACATTCCAAATGCAAGCAATGCTTGCTCGGGATTTGATAATTCGTTTGGGTGGTCTTTCTTTGATTTGAATGCAATTCATGATGACCACAAAGATAGTACAGAAACAGTGCTACAAGCTGATTTTTATCGTGCCAATGAACTGTTAGTTCTGACAGATAAGCAAATAGTTGCCAAGGTGACATCTTACCTGTCAAACTGCATCAAGGACTTCGAGAATGCTACTGTGAAAAATAAGGAGCTCGGAAGATTTCCAAAATCATCAACTCATTTCTTTCCAGGCTCATATAAGTACATGATTCGCGGGTCTACATCTTTTCCAAACTTGTTCATGGCTGGAGACTGGATTATAACTCGACATGGTTCATGGTCACAGGAGAAGTCTTATGTCACCGGTCTAGAAGCTGCCAACCGTACAGTGGACTATCTAGAAGAAGGAAGCTTTGCCAAAATAATACCTGTTGAGGAGGATGAACCACATATTCAGACACTTCGTAGCTTAAACAGAAGTTTTAATGATATCAAAGCCCAATTACCGTTATCTAGTTATTTCCTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

544

Amino Acids

60.76

Weight (kDa)

5.58

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amino_oxidase PF01593 104 - 495 1.9e-18 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1421
Acc16I TGCGCA 1 cut(s) 387
AccI GTMKAC 2 cut(s) 989, 1379
AccII CGCG 2 cut(s) 61, 1374
AciI CCGC 2 cut(s) 59, 1374
AcsI RAATTY 2 cut(s) 416, 1001
AcuI CTGAAG 3 cut(s) 165, 909, 941
AfaI GTAC 5 cut(s) 470, 1038, 1171, 1364, 1486
AfiI CCNNNNNNNGG 2 cut(s) 149, 1489
AgeI ACCGGT 1 cut(s) 1463
AgsI TTSAA 8 cut(s) 67, 266, 416, 431, 582, 659, 971, 1141
AhlI ACTAGT 1 cut(s) 37
AjnI CCWGG 2 cut(s) 1020, 1348
Alw21I GWGCWC 2 cut(s) 112, 1314
Alw26I GTCTC 2 cut(s) 1018, 1404
AlwNI CAGNNNCTG 1 cut(s) 143
Ama87I CYCGRG 1 cut(s) 1099
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 7 cut(s) 102, 218, 530, 615, 695, 698, 1475
ApoI RAATTY 2 cut(s) 416, 1001
AsiGI ACCGGT 1 cut(s) 1463
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 2 cut(s) 218, 388
AspS9I GGNCC 2 cut(s) 152, 375
AsuHPI GGTGA 4 cut(s) 725, 1081, 1261, 1453
AvaI CYCGRG 1 cut(s) 1099
AvaII GGWCC 2 cut(s) 152, 375
BanII GRGCYC 2 cut(s) 112, 1314
Bbv12I GWGCWC 2 cut(s) 112, 1314
BbvI GCAGC 7 cut(s) 114, 205, 517, 602, 682, 685, 1462
BccI CCATC 2 cut(s) 143, 195
BceAI ACGGC 1 cut(s) 747
BciT130I CCWGG 2 cut(s) 1022, 1350
BciVI GTATCC 2 cut(s) 324, 1018
BcoDI GTCTC 2 cut(s) 1018, 1404
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 9 cut(s) 38, 44, 128, 644, 752, 774, 1469, 1499, 1614
BfmI CTRYAG 2 cut(s) 396, 531
BfoI RGCGCY 1 cut(s) 219
BfuI GTATCC 2 cut(s) 324, 1018
BglII AGATCT 1 cut(s) 783
BisI GCNGC 7 cut(s) 103, 219, 531, 616, 696, 699, 1476
BlsI GCNGC 7 cut(s) 104, 220, 532, 617, 697, 700, 1477
Bme1390I CCNGG 2 cut(s) 1022, 1350
Bme18I GGWCC 2 cut(s) 152, 375
BmeT110I CYCGRG 1 cut(s) 1099
BmgT120I GGNCC 2 cut(s) 152, 375
BmiI GGNNCC 1 cut(s) 463
BmrFI CCNGG 2 cut(s) 1022, 1350
BmrI ACTGGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 913, 1281
BmuI ACTGGG 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 13, 45
BpmI CTGGAG 1 cut(s) 1428
BpuEI CTTGAG 2 cut(s) 102, 371
BsaBI GATNNNNATC 2 cut(s) 747, 1197
BsaI GGTCTC 1 cut(s) 1018
BsaJI CCNNGG 5 cut(s) 211, 301, 763, 794, 1245
BsaWI WCCGGW 3 cut(s) 321, 421, 1463
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 1489
Bse118I RCCGGY 1 cut(s) 1463
Bse1I ACTGG 4 cut(s) 88, 283, 377, 1418
Bse3DI GCAATG 2 cut(s) 650, 1096
Bse8I GATNNNNATC 2 cut(s) 747, 1197
BseBI CCWGG 2 cut(s) 1022, 1350
BseDI CCNNGG 5 cut(s) 211, 301, 763, 794, 1245
BseGI GGATG 3 cut(s) 206, 723, 1546
BseJI GATNNNNATC 2 cut(s) 747, 1197
BseLI CCNNNNNNNGG 2 cut(s) 149, 1489
BseMI GCAATG 2 cut(s) 650, 1096
BseMII CTCAG 2 cut(s) 455, 633
BseNI ACTGG 4 cut(s) 88, 283, 377, 1418
BseRI GAGGAG 1 cut(s) 1550
BseXI GCAGC 7 cut(s) 114, 205, 517, 602, 682, 685, 1462
BsgI GTGCAG 2 cut(s) 498, 999
Bsh1236I CGCG 2 cut(s) 61, 1374
BshFI GGCC 1 cut(s) 210
BshTI ACCGGT 1 cut(s) 1463
BsiHKAI GWGCWC 2 cut(s) 112, 1314
BsiHKCI CYCGRG 1 cut(s) 1099
BsiSI CCGG 3 cut(s) 322, 422, 1464
BslFI GGGAC 2 cut(s) 32, 125
BslI CCNNNNNNNGG 2 cut(s) 149, 1489
BsmAI GTCTC 2 cut(s) 1018, 1404
BsmFI GGGAC 2 cut(s) 32, 125
BsmI GAATGC 2 cut(s) 1147, 1294
BsnI GGCC 1 cut(s) 210
Bso31I GGTCTC 1 cut(s) 1018
BsoBI CYCGRG 1 cut(s) 1099
Bsp1286I GDGCHC 2 cut(s) 112, 1314
Bsp143I GATC 2 cut(s) 748, 783
Bsp19I CCATGG 1 cut(s) 301
BspACI CCGC 2 cut(s) 59, 1374
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 2 cut(s) 454, 632
BspFNI CGCG 2 cut(s) 61, 1374
BspHI TCATGA 1 cut(s) 1150
BspLI GGNNCC 1 cut(s) 463
BspMAI CTGCAG 2 cut(s) 400, 535
BspTNI GGTCTC 1 cut(s) 1018
BsrDI GCAATG 2 cut(s) 650, 1096
BsrFI RCCGGY 1 cut(s) 1463
BsrI ACTGG 4 cut(s) 88, 283, 377, 1418
BssAI RCCGGY 1 cut(s) 1463
BssECI CCNNGG 5 cut(s) 211, 301, 763, 794, 1245
BssMI GATC 2 cut(s) 748, 783
BssT1I CCWWGG 4 cut(s) 211, 301, 794, 1245
Bst2UI CCWGG 2 cut(s) 1022, 1350
Bst4CI ACNGT 9 cut(s) 139, 259, 480, 1180, 1215, 1297, 1484, 1489, 1608
BstAPI GCANNNNNTGC 2 cut(s) 1091, 1095
BstC8I GCNNGC 4 cut(s) 208, 713, 1087, 1096
BstDEI CTNAG 2 cut(s) 441, 619
BstDSI CCRYGG 2 cut(s) 301, 763
BstF5I GGATG 3 cut(s) 206, 723, 1546
BstFNI CGCG 2 cut(s) 61, 1374
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 2 cut(s) 218, 388
BstKTI GATC 2 cut(s) 751, 786
BstMAI GTCTC 2 cut(s) 1018, 1404
BstMBI GATC 2 cut(s) 748, 783
BstMWI GCNNNNNNNGC 2 cut(s) 1091, 1095
BstNI CCWGG 2 cut(s) 1022, 1350
BstSCI CCNGG 2 cut(s) 1020, 1348
BstSFI CTRYAG 2 cut(s) 396, 531
BstUI CGCG 2 cut(s) 61, 1374
BstV1I GCAGC 7 cut(s) 114, 205, 517, 602, 682, 685, 1462
BstX2I RGATCY 1 cut(s) 783
BstYI RGATCY 1 cut(s) 783
BsuI GTATCC 2 cut(s) 324, 1018
BsuRI GGCC 1 cut(s) 210
BtgI CCRYGG 2 cut(s) 301, 763
BtsCI GGATG 3 cut(s) 206, 723, 1546
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 4 cut(s) 366, 987, 1185, 1494
Cac8I GCNNGC 4 cut(s) 208, 713, 1087, 1096
CaiI CAGNNNCTG 1 cut(s) 143
CciI TCATGA 1 cut(s) 1150
CfoI GCGC 2 cut(s) 218, 388
Cfr10I RCCGGY 1 cut(s) 1463
Cfr13I GGNCC 2 cut(s) 152, 375
Csp6I GTAC 5 cut(s) 469, 1037, 1170, 1363, 1485
CspAI ACCGGT 1 cut(s) 1463
CviAII CATG 6 cut(s) 302, 1151, 1366, 1402, 1430, 1437
CviQI GTAC 5 cut(s) 469, 1037, 1170, 1363, 1485
DdeI CTNAG 2 cut(s) 441, 619
DpnI GATC 2 cut(s) 750, 785
DpnII GATC 2 cut(s) 748, 783
Ecl136II GAGCTC 2 cut(s) 110, 1312
Eco130I CCWWGG 4 cut(s) 211, 301, 794, 1245
Eco24I GRGCYC 2 cut(s) 112, 1314
Eco31I GGTCTC 1 cut(s) 1018
Eco32I GATATC 1 cut(s) 1591
Eco47I GGWCC 2 cut(s) 152, 375
Eco53kI GAGCTC 2 cut(s) 110, 1312
Eco57I CTGAAG 3 cut(s) 165, 909, 941
Eco88I CYCGRG 1 cut(s) 1099
EcoICRI GAGCTC 2 cut(s) 110, 1312
EcoRII CCWGG 2 cut(s) 1020, 1348
EcoRV GATATC 1 cut(s) 1591
EcoT14I CCWWGG 4 cut(s) 211, 301, 794, 1245
EcoT38I GRGCYC 2 cut(s) 112, 1314
ErhI CCWWGG 4 cut(s) 211, 301, 794, 1245
FaeI CATG 6 cut(s) 305, 1154, 1369, 1405, 1433, 1440
FalI AAGNNNNNCTT 2 cut(s) 1078, 1110
FaqI GGGAC 2 cut(s) 32, 125
FatI CATG 6 cut(s) 301, 1150, 1365, 1401, 1429, 1436
FauI CCCGC 1 cut(s) 1367
FblI GTMKAC 2 cut(s) 989, 1379
Fnu4HI GCNGC 7 cut(s) 103, 219, 531, 616, 696, 699, 1476
FokI GGATG 3 cut(s) 213, 710, 1553
FriOI GRGCYC 2 cut(s) 112, 1314
Fsp4HI GCNGC 7 cut(s) 103, 219, 531, 616, 696, 699, 1476
FspBI CTAG 9 cut(s) 38, 44, 128, 644, 752, 774, 1469, 1499, 1614
FspI TGCGCA 1 cut(s) 387
GlaI GCGC 2 cut(s) 217, 387
GluI GCNGC 7 cut(s) 103, 219, 531, 616, 696, 699, 1476
GsuI CTGGAG 1 cut(s) 1428
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 1 cut(s) 210
HapII CCGG 3 cut(s) 322, 422, 1464
HhaI GCGC 2 cut(s) 218, 388
Hin1II CATG 6 cut(s) 305, 1154, 1369, 1405, 1433, 1440
Hin6I GCGC 2 cut(s) 216, 386
HinP1I GCGC 2 cut(s) 216, 386
HindIII AAGCTT 2 cut(s) 626, 1510
HinfI GANTC 5 cut(s) 24, 578, 803, 945, 1369
HpaII CCGG 3 cut(s) 322, 422, 1464
HphI GGTGA 4 cut(s) 725, 1081, 1261, 1453
Hpy166II GTNNAC 4 cut(s) 990, 1072, 1380, 1492
Hpy188I TCNGA 7 cut(s) 622, 636, 889, 960, 1224, 1316, 1557
Hpy188III TCNNGA 9 cut(s) 198, 263, 284, 1015, 1101, 1151, 1285, 1469, 1499
Hpy8I GTNNAC 4 cut(s) 990, 1072, 1380, 1492
Hpy99I CGWCG 3 cut(s) 102, 164, 191
HpyAV CCTTC 4 cut(s) 172, 373, 1060, 1499
HpyCH4III ACNGT 9 cut(s) 139, 259, 480, 1180, 1215, 1297, 1484, 1489, 1608
HpyF10VI GCNNNNNNNGC 2 cut(s) 1091, 1095
HpyF3I CTNAG 2 cut(s) 441, 619
Hsp92II CATG 6 cut(s) 305, 1154, 1369, 1405, 1433, 1440
HspAI GCGC 2 cut(s) 216, 386
Kzo9I GATC 2 cut(s) 748, 783
LmnI GCTCC 2 cut(s) 115, 1309
Lsp1109I GCAGC 7 cut(s) 114, 205, 517, 602, 682, 685, 1462
LweI GCATC 2 cut(s) 913, 1281
MaeI CTAG 9 cut(s) 38, 44, 128, 644, 752, 774, 1469, 1499, 1614
MaeIII GTNAC 5 cut(s) 253, 847, 1249, 1440, 1459
MalI GATC 2 cut(s) 750, 785
MboI GATC 2 cut(s) 748, 783
MboII GAAGA 4 cut(s) 158, 187, 1329, 1514
MflI RGATCY 1 cut(s) 783
MhlI GDGCHC 2 cut(s) 112, 1314
MluCI AATT 6 cut(s) 416, 582, 1001, 1111, 1146, 1601
MlyI GAGTC 1 cut(s) 812
MmeI TCCRAC 1 cut(s) 922
MnlI CCTC 9 cut(s) 39, 239, 394, 403, 450, 862, 991, 1528, 1531
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 9 cut(s) 357, 509, 524, 603, 789, 864, 1047, 1571, 1584
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 3 cut(s) 322, 422, 1464
MspR9I CCNGG 2 cut(s) 1022, 1350
Mva1269I GAATGC 2 cut(s) 1147, 1294
MvaI CCWGG 2 cut(s) 1022, 1350
MvnI CGCG 2 cut(s) 61, 1374
MwoI GCNNNNNNNGC 2 cut(s) 1091, 1095
NcoI CCATGG 1 cut(s) 301
NdeII GATC 2 cut(s) 748, 783
NlaIII CATG 6 cut(s) 305, 1154, 1369, 1405, 1433, 1440
NlaIV GGNNCC 1 cut(s) 463
NmeAIII GCCGAG 1 cut(s) 687
NmuCI GTSAC 3 cut(s) 1249, 1440, 1459
NsbI TGCGCA 1 cut(s) 387
PagI TCATGA 1 cut(s) 1150
PctI GAATGC 2 cut(s) 1147, 1294
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 4 cut(s) 24, 578, 945, 1369
PinAI ACCGGT 1 cut(s) 1463
PkrI GCNGC 7 cut(s) 104, 220, 532, 617, 697, 700, 1477
PleI GAGTC 1 cut(s) 811
PpsI GAGTC 1 cut(s) 811
PsiI TTATAA 1 cut(s) 1421
Psp124BI GAGCTC 2 cut(s) 112, 1314
Psp6I CCWGG 2 cut(s) 1020, 1348
PspGI CCWGG 2 cut(s) 1020, 1348
PspN4I GGNNCC 1 cut(s) 463
PspPI GGNCC 2 cut(s) 152, 375
PstI CTGCAG 2 cut(s) 400, 535
PstNI CAGNNNCTG 1 cut(s) 143
PsuI RGATCY 1 cut(s) 783
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 5 cut(s) 470, 1038, 1171, 1364, 1486
RsaNI GTAC 5 cut(s) 469, 1037, 1170, 1363, 1485
SacI GAGCTC 2 cut(s) 112, 1314
SaqAI TTAA 9 cut(s) 357, 509, 524, 603, 789, 864, 1047, 1571, 1584
SatI GCNGC 7 cut(s) 103, 219, 531, 616, 696, 699, 1476
Sau3AI GATC 2 cut(s) 748, 783
Sau96I GGNCC 2 cut(s) 152, 375
SchI GAGTC 1 cut(s) 812
ScrFI CCNGG 2 cut(s) 1022, 1350
SduI GDGCHC 2 cut(s) 112, 1314
SfaNI GCATC 2 cut(s) 913, 1281
SfcI CTRYAG 2 cut(s) 396, 531
SinI GGWCC 2 cut(s) 152, 375
SmlI CTYRAG 2 cut(s) 117, 350
SmoI CTYRAG 2 cut(s) 117, 350
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 6 cut(s) 416, 582, 1001, 1111, 1146, 1601
SsiI CCGC 2 cut(s) 59, 1374
SspI AATATT 1 cut(s) 392
SspMI CTAG 9 cut(s) 38, 44, 128, 644, 752, 774, 1469, 1499, 1614
SstI GAGCTC 2 cut(s) 112, 1314
StyD4I CCNGG 2 cut(s) 1020, 1348
StyI CCWWGG 4 cut(s) 211, 301, 794, 1245
TaaI ACNGT 9 cut(s) 139, 259, 480, 1180, 1215, 1297, 1484, 1489, 1608
TaqI TCGA 5 cut(s) 540, 1016, 1059, 1284, 1426
TasI AATT 6 cut(s) 416, 582, 1001, 1111, 1146, 1601
TatI WGTACW 4 cut(s) 468, 1036, 1169, 1362
TfiI GAWTC 4 cut(s) 24, 578, 945, 1369
Tru1I TTAA 9 cut(s) 357, 509, 524, 603, 789, 864, 1047, 1571, 1584
Tru9I TTAA 9 cut(s) 357, 509, 524, 603, 789, 864, 1047, 1571, 1584
TscAI CASTG 4 cut(s) 373, 987, 1185, 1494
TseFI GTSAC 3 cut(s) 1249, 1440, 1459
TseI GCWGC 7 cut(s) 102, 218, 530, 615, 695, 698, 1475
Tsp45I GTSAC 3 cut(s) 1249, 1440, 1459
TspDTI ATGAA 5 cut(s) 1139, 1224, 1390, 1425, 1557
TspRI CASTG 4 cut(s) 373, 987, 1185, 1494
VpaK11BI GGWCC 2 cut(s) 152, 375
XapI RAATTY 2 cut(s) 416, 1001
XbaI TCTAGA 2 cut(s) 1468, 1498
XcmI CCANNNNNNNNNTGG 1 cut(s) 1486
XmiI GTMKAC 2 cut(s) 989, 1379
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 9 cut(s) 38, 44, 128, 644, 752, 774, 1469, 1499, 1614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.