Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012576

Physical Location & Seq

Genomic Coordinates Forward (+)

24677226 .. 24681236

Gene Structure 4011 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1124600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGTCGTTGGCTGCCTGTTGGAGGCTGACTCTGACATGCCCGCCACCGACCTCCTCGCGGACTTCATCCTGCTTCTCTCCCAGAAGCCGAAGTGGGTTCCGTTGCAGAGCTTCTTCTGGGCAAGCCCACGCTGCAATTGGGGGCCCAAACGACAAGGAAAAGAAGAAGGTTGTCGTTGTTGGCTCTGGCTGGGCTGGCCTCGGCGCTGCTCACCACCTCTGCAACCAGGGTTTTGATGTTACTGTTATCGAACAAGGTAGTGGCTTGGGCGTACTTCATGAAATTGGTATCCAGCGCGGTAATTGGTATCCTTTCCGGAATATTTTTAGCCTTGTTGATGAGCTAGGTATCAAACCCTTTACTAACTGGACAAAGTTTGCACAATATTCAGCAGAAGGGTTGGAGGCTGAGTTTCCGGTATTTCAGGACCTTCCTCAATTGCCAGCACCATTTGGAACCTTATACTATACACAGTTTGCTCAGCTGCCATTGGTTGATAGATTAACTTCACTTCCCTTAACAGCTGCAGTGATCGATTTTGACAACACAGATACAGCCTGGAGAAAATACGATGCAATTACTGCAAGGGAGCTTTTTAAGCAGTTTGGATGTTCGGAAAACCTTTATCAGAATGTCTTATCACCATTGATTCAAGTGGGTCTTTCTGCACCGGCTGAACAATGTAGTGCTGCCGCAACTCTTGGTTTACTATCCTACATCTTTGCTCACCAGAAAAATTTTGATCTCGTTTTGTGTCGTGGAACAAGTAGAGAAAGGATTTTCAAGCCATGGATGGAGTCTTTGACAACTAAAGGTTGCAAATTCGAGAAGGGTATGCAATTAACAGACTTTGTGCTTAATGAGGAAACTGGTTCTATTTCAGAAGTAGTTTGCAATGGAGGAGTTTATAATGCTGATGCAGTTGTCTTAGCTATTGGAATATCCACACTCCAAAAAATTATTGAAAACAGTGCAGTGTTGTGTACAAGGGAGGAGTTTCTCAAAGTCTTGAACTTGGCTAGCATTGATGCACTCTCTGTTAAGCTTTGGCTTGATAGGAAGGTTAACATTCCAAATGCATGCAATGCTTGCTCCGGATTTGACGATTCATTTGGTTGGACTTTCTTTGATTTGAATGCAATCTATGACGATCATAAAGATAGTTCGGTAACAGTGTTACAAGCTGATTTTTATCGCGCCAACGAGTTGTTGCCACTGAAAGATGAGCAAATAGTCTCTCGAGCAGTATCCTACCTGTCAAAGTACATTAAAGACTTCAAGAATGTTACTGTGACAAACAAGAAGATTGGCAGATTTCCAAAATCATTACCTCACTTTTTTCCCGGTTCATATAAGGACATGATGCGTGGGGCTACATCTTTCCAGAACTTGTTCATGGCGGGAGACTGGATTATAACCCGACATGGTTCATGGTCACAGGAGAAAACTTACGTGACGGGACTTGAAGCTGCGAATCGCGCAGTAGACTACCTAGAAGAAGGAAGTTTTGCGAAAATAATACCTGTAGAGGAAGATGAACCGCACATTCAAGCGCTTCGCAGCCTGAACAGAAGCTTCAAGGAGATCACAGCACAGCTTCCGTTGTCCAGTTACTTCCTCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Amino_oxidase NAD_binding_8

Protein Analysis

542

Amino Acids

60.16

Weight (kDa)

6.34

Isoelectric Point (pI)

37.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAO	PF01266	56 - 90	1.2e-06	FAD dependent oxidoreductase
NAD_binding_8	PF13450	59 - 90	7.9e-08	NAD(P)-binding Rossmann-like domain
Amino_oxidase	PF01593	65 - 493	3.5e-18	Flavin containing amine oxidoreductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012576)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g15600
malus_domestica	MD05G1124600.v1.1
prunus_persica	Prupe.8G168300_v2.0.a1
pyrus_communis	pycom05g11860
rosa_chinensis	RchiOBHm_Chr6g0255801 RchiOBHm_Chr6g0278781 RchiOBHm_Chr6g0278801
rosa_laevigata	RLG00000013193
rosa_multiflora	Rmu_sc0004380.1_g000003
rosa_roxburghii	Rroxscaffold_5G00353520 Rroxscaffold_7G00189630 Rroxscaffold_7G00189900
rosa_rugosa	Rorug06G0119000
rosa_samantha	Rh5BG115500 Rh6AG227700 Rh6BG233300 Rh6CG235800 Rh6DG227000
rosa_wichuraiana	Rw6G019980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	909, 1415
AccI	GTMKAC	1 cut(s)	1485
AccII	CGCG	4 cut(s)	58, 297, 1197, 1479
AccIII	TCCGGA	2 cut(s)	315, 1094
AciI	CCGC	6 cut(s)	41, 58, 297, 693, 1400, 1541
AcsI	RAATTY	2 cut(s)	736, 821
AfaI	GTAC	3 cut(s)	273, 985, 1265
AfeI	AGCGCT	1 cut(s)	1554
AfiI	CCNNNNNNNGG	2 cut(s)	21, 57
AgsI	TTSAA	9 cut(s)	653, 784, 965, 1012, 1135, 1279, 1466, 1550, 1579
AjnI	CCWGG	2 cut(s)	225, 557
Alw26I	GTCTC	2 cut(s)	1240, 1398
Ama87I	CYCGRG	1 cut(s)	1239
Aor13HI	TCCGGA	2 cut(s)	315, 1094
Aor51HI	AGCGCT	1 cut(s)	1554
AoxI	GGCC	2 cut(s)	142, 196
ApaI	GGGCCC	1 cut(s)	146
ApeKI	GCWGC	8 cut(s)	11, 131, 206, 484, 524, 689, 1469, 1560
ApoI	RAATTY	2 cut(s)	736, 821
ArsI	GACNNNNNNTTYG	2 cut(s)	1094, 1126
Asp700I	GAANNNNTTC	1 cut(s)	1391
AspLEI	GCGC	5 cut(s)	206, 297, 1199, 1481, 1555
AspS9I	GGNCC	3 cut(s)	142, 143, 427
AsuC2I	CCSGG	1 cut(s)	1344
AsuHPI	GGTGA	3 cut(s)	203, 633, 719
AsuNHI	GCTAGC	1 cut(s)	1019
AvaI	CYCGRG	1 cut(s)	1239
AvaII	GGWCC	1 cut(s)	427
BaeGI	GKGCMC	1 cut(s)	146
BanII	GRGCYC	1 cut(s)	146
BbvI	GCAGC	7 cut(s)	118, 193, 471, 511, 676, 1456, 1572
BccI	CCATC	1 cut(s)	787
BciT130I	CCWGG	2 cut(s)	227, 559
BciVI	GTATCC	3 cut(s)	299, 318, 1258
BcnI	CCSGG	1 cut(s)	1344
BcoDI	GTCTC	2 cut(s)	1240, 1398
BfaI	CTAG	3 cut(s)	344, 1020, 1493
BfmI	CTRYAG	2 cut(s)	525, 1524
BfoI	RGCGCY	2 cut(s)	207, 1556
BfuI	GTATCC	3 cut(s)	299, 318, 1258
BisI	GCNGC	9 cut(s)	12, 132, 207, 485, 525, 690, 693, 1470, 1561
BlpI	GCTNAGC	1 cut(s)	480
BlsI	GCNGC	9 cut(s)	13, 133, 208, 486, 526, 691, 694, 1471, 1562
Bme1390I	CCNGG	3 cut(s)	227, 559, 1344
Bme18I	GGWCC	1 cut(s)	427
BmeT110I	CYCGRG	1 cut(s)	1239
BmgT120I	GGNCC	3 cut(s)	142, 143, 427
BmiI	GGNNCC	4 cut(s)	98, 143, 144, 457
BmrFI	CCNGG	3 cut(s)	227, 559, 1344
BmsI	GCATC	4 cut(s)	562, 907, 1018, 1353
BmtI	GCTAGC	1 cut(s)	1023
BplI	GAGNNNNNCTC	2 cut(s)	13, 45
BpmI	CTGGAG	1 cut(s)	580
Bpu1102I	GCTNAGC	1 cut(s)	480
BpuMI	CCSGG	1 cut(s)	1344
Bsa29I	ATCGAT	1 cut(s)	534
BsaAI	YACGTR	1 cut(s)	1453
BsaBI	GATNNNNATC	1 cut(s)	1191
BsaJI	CCNNGG	3 cut(s)	199, 226, 788
BsaWI	WCCGGW	3 cut(s)	315, 415, 1094
BsaXI	ACNNNNNCTCC	2 cut(s)	395, 425
Bsc4I	CCNNNNNNNGG	2 cut(s)	21, 57
Bse118I	RCCGGY	1 cut(s)	670
Bse1I	ACTGG	4 cut(s)	371, 874, 1412, 1608
Bse3DI	GCAATG	2 cut(s)	901, 1090
Bse8I	GATNNNNATC	1 cut(s)	1191
BseAI	TCCGGA	2 cut(s)	315, 1094
BseBI	CCWGG	2 cut(s)	227, 559
BseCI	ATCGAT	1 cut(s)	534
BseDI	CCNNGG	3 cut(s)	199, 226, 788
BseGI	GGATG	3 cut(s)	65, 614, 798
BseJI	GATNNNNATC	1 cut(s)	1191
BseLI	CCNNNNNNNGG	2 cut(s)	21, 57
BseMI	GCAATG	2 cut(s)	901, 1090
BseMII	CTCAG	2 cut(s)	399, 494
BseNI	ACTGG	4 cut(s)	371, 874, 1412, 1608
BseRI	GAGGAG	3 cut(s)	43, 915, 1007
BseSI	GKGCMC	1 cut(s)	146
BseXI	GCAGC	7 cut(s)	118, 193, 471, 511, 676, 1456, 1572
BseYI	CCCAGC	1 cut(s)	189
BsgI	GTGCAG	2 cut(s)	651, 993
Bsh1236I	CGCG	4 cut(s)	58, 297, 1197, 1479
BshFI	GGCC	2 cut(s)	144, 198
BshVI	ATCGAT	1 cut(s)	534
BsiHKCI	CYCGRG	1 cut(s)	1239
BsiSI	CCGG	5 cut(s)	316, 416, 671, 1095, 1344
BslFI	GGGAC	1 cut(s)	1473
BslI	CCNNNNNNNGG	2 cut(s)	21, 57
BsmAI	GTCTC	2 cut(s)	1240, 1398
BsmFI	GGGAC	1 cut(s)	1473
BsmI	GAATGC	1 cut(s)	1141
BsnI	GGCC	2 cut(s)	144, 198
BsoBI	CYCGRG	1 cut(s)	1239
Bsp120I	GGGCCC	1 cut(s)	142
Bsp1286I	GDGCHC	1 cut(s)	146
Bsp13I	TCCGGA	2 cut(s)	315, 1094
Bsp1407I	TGTACA	1 cut(s)	983
Bsp143I	GATC	4 cut(s)	531, 742, 1150, 1584
Bsp1720I	GCTNAGC	1 cut(s)	480
Bsp19I	CCATGG	1 cut(s)	788
BspACI	CCGC	6 cut(s)	41, 58, 297, 693, 1400, 1541
BspANI	GGCC	2 cut(s)	144, 198
BspCNI	CTCAG	2 cut(s)	400, 493
BspDI	ATCGAT	1 cut(s)	534
BspEI	TCCGGA	2 cut(s)	315, 1094
BspFNI	CGCG	4 cut(s)	58, 297, 1197, 1479
BspHI	TCATGA	1 cut(s)	277
BspLI	GGNNCC	4 cut(s)	98, 143, 144, 457
BspMAI	CTGCAG	1 cut(s)	529
BspOI	GCTAGC	1 cut(s)	1023
BsrDI	GCAATG	2 cut(s)	901, 1090
BsrFI	RCCGGY	1 cut(s)	670
BsrGI	TGTACA	1 cut(s)	983
BsrI	ACTGG	4 cut(s)	371, 874, 1412, 1608
BssAI	RCCGGY	1 cut(s)	670
BssECI	CCNNGG	3 cut(s)	199, 226, 788
BssMI	GATC	4 cut(s)	531, 742, 1150, 1584
BssT1I	CCWWGG	1 cut(s)	788
Bst2UI	CCWGG	2 cut(s)	227, 559
Bst4CI	ACNGT	5 cut(s)	244, 474, 971, 1174, 1291
BstAPI	GCANNNNNTGC	3 cut(s)	581, 1085, 1089
BstAUI	TGTACA	1 cut(s)	983
BstBAI	YACGTR	1 cut(s)	1453
BstC8I	GCNNGC	7 cut(s)	41, 123, 196, 444, 1021, 1081, 1090
BstDEI	CTNAG	3 cut(s)	408, 480, 928
BstDSI	CCRYGG	1 cut(s)	788
BstENI	CCTNNNNNAGG	1 cut(s)	19
BstF5I	GGATG	3 cut(s)	65, 614, 798
BstFNI	CGCG	4 cut(s)	58, 297, 1197, 1479
BstH2I	RGCGCY	2 cut(s)	207, 1556
BstHHI	GCGC	5 cut(s)	206, 297, 1199, 1481, 1555
BstKTI	GATC	4 cut(s)	534, 745, 1153, 1587
BstMAI	GTCTC	2 cut(s)	1240, 1398
BstMBI	GATC	4 cut(s)	531, 742, 1150, 1584
BstMWI	GCNNNNNNNGC	7 cut(s)	131, 195, 581, 598, 1085, 1089, 1478
BstNI	CCWGG	2 cut(s)	227, 559
BstNSI	RCATGY	2 cut(s)	39, 1083
BstSCI	CCNGG	3 cut(s)	225, 557, 1342
BstSFI	CTRYAG	2 cut(s)	525, 1524
BstSLI	GKGCMC	1 cut(s)	146
BstUI	CGCG	4 cut(s)	58, 297, 1197, 1479
BstV1I	GCAGC	7 cut(s)	118, 193, 471, 511, 676, 1456, 1572
Bsu15I	ATCGAT	1 cut(s)	534
BsuI	GTATCC	3 cut(s)	299, 318, 1258
BsuRI	GGCC	2 cut(s)	144, 198
BsuTUI	ATCGAT	1 cut(s)	534
BtgI	CCRYGG	1 cut(s)	788
BtsCI	GGATG	3 cut(s)	65, 614, 798
BtsI	GCAGTG	2 cut(s)	534, 981
BtsIMutI	CAGTG	5 cut(s)	534, 976, 981, 1179, 1214
Cac8I	GCNNGC	7 cut(s)	41, 123, 196, 444, 1021, 1081, 1090
CciI	TCATGA	1 cut(s)	277
CfoI	GCGC	5 cut(s)	206, 297, 1199, 1481, 1555
Cfr10I	RCCGGY	1 cut(s)	670
Cfr13I	GGNCC	3 cut(s)	142, 143, 427
ClaI	ATCGAT	1 cut(s)	534
Csp6I	GTAC	3 cut(s)	272, 984, 1264
CviAII	CATG	8 cut(s)	36, 278, 789, 1080, 1360, 1396, 1424, 1431
CviQI	GTAC	3 cut(s)	272, 984, 1264
DdeI	CTNAG	3 cut(s)	408, 480, 928
DpnI	GATC	4 cut(s)	533, 744, 1152, 1586
DpnII	GATC	4 cut(s)	531, 742, 1150, 1584
Eco130I	CCWWGG	1 cut(s)	788
Eco24I	GRGCYC	1 cut(s)	146
Eco47I	GGWCC	1 cut(s)	427
Eco47III	AGCGCT	1 cut(s)	1554
Eco88I	CYCGRG	1 cut(s)	1239
EcoNI	CCTNNNNNAGG	1 cut(s)	19
EcoO109I	RGGNCCY	2 cut(s)	142, 427
EcoRII	CCWGG	2 cut(s)	225, 557
EcoT14I	CCWWGG	1 cut(s)	788
EcoT22I	ATGCAT	1 cut(s)	1081
EcoT38I	GRGCYC	1 cut(s)	146
ErhI	CCWWGG	1 cut(s)	788
FaeI	CATG	8 cut(s)	39, 281, 792, 1083, 1363, 1399, 1427, 1434
FalI	AAGNNNNNCTT	2 cut(s)	645, 677
FaqI	GGGAC	1 cut(s)	1473
FatI	CATG	8 cut(s)	35, 277, 788, 1079, 1359, 1395, 1423, 1430
FauI	CCCGC	2 cut(s)	48, 1393
FblI	GTMKAC	1 cut(s)	1485
Fnu4HI	GCNGC	9 cut(s)	12, 132, 207, 485, 525, 690, 693, 1470, 1561
FokI	GGATG	3 cut(s)	52, 621, 805
FriOI	GRGCYC	1 cut(s)	146
Fsp4HI	GCNGC	9 cut(s)	12, 132, 207, 485, 525, 690, 693, 1470, 1561
FspBI	CTAG	3 cut(s)	344, 1020, 1493
GlaI	GCGC	5 cut(s)	205, 296, 1198, 1480, 1554
GluI	GCNGC	9 cut(s)	12, 132, 207, 485, 525, 690, 693, 1470, 1561
GsaI	CCCAGC	1 cut(s)	193
GsuI	CTGGAG	1 cut(s)	580
HaeII	RGCGCY	2 cut(s)	207, 1556
HaeIII	GGCC	2 cut(s)	144, 198
HapII	CCGG	5 cut(s)	316, 416, 671, 1095, 1344
HhaI	GCGC	5 cut(s)	206, 297, 1199, 1481, 1555
Hin1II	CATG	8 cut(s)	39, 281, 792, 1083, 1363, 1399, 1427, 1434
Hin6I	GCGC	5 cut(s)	204, 295, 1197, 1479, 1553
HinP1I	GCGC	5 cut(s)	204, 295, 1197, 1479, 1553
HincII	GTYRAC	1 cut(s)	1066
HindII	GTYRAC	1 cut(s)	1066
HindIII	AAGCTT	2 cut(s)	1043, 1573
HinfI	GANTC	5 cut(s)	28, 649, 797, 1106, 1474
HpaI	GTTAAC	1 cut(s)	1066
HpaII	CCGG	5 cut(s)	316, 416, 671, 1095, 1344
HphI	GGTGA	3 cut(s)	203, 633, 719
Hpy166II	GTNNAC	4 cut(s)	707, 984, 1066, 1486
Hpy188I	TCNGA	4 cut(s)	33, 616, 630, 883
Hpy188III	TCNNGA	9 cut(s)	278, 316, 425, 826, 1009, 1095, 1239, 1279, 1384
Hpy8I	GTNNAC	4 cut(s)	707, 984, 1066, 1486
HpyAV	CCTTC	6 cut(s)	160, 389, 440, 823, 1054, 1493
HpyCH4III	ACNGT	5 cut(s)	244, 474, 971, 1174, 1291
HpyCH4IV	ACGT	1 cut(s)	1452
HpyF10VI	GCNNNNNNNGC	7 cut(s)	131, 195, 581, 598, 1085, 1089, 1478
HpyF3I	CTNAG	3 cut(s)	408, 480, 928
HpySE526I	ACGT	1 cut(s)	1452
Hsp92II	CATG	8 cut(s)	39, 281, 792, 1083, 1363, 1399, 1427, 1434
HspAI	GCGC	5 cut(s)	204, 295, 1197, 1479, 1553
Kpn2I	TCCGGA	2 cut(s)	315, 1094
KspAI	GTTAAC	1 cut(s)	1066
Kzo9I	GATC	4 cut(s)	531, 742, 1150, 1584
LmnI	GCTCC	2 cut(s)	589, 1097
Lsp1109I	GCAGC	7 cut(s)	118, 193, 471, 511, 676, 1456, 1572
LweI	GCATC	4 cut(s)	562, 907, 1018, 1353
MaeI	CTAG	3 cut(s)	344, 1020, 1493
MaeII	ACGT	1 cut(s)	1452
MaeIII	GTNAC	8 cut(s)	238, 1168, 1176, 1285, 1291, 1434, 1453, 1610
MalI	GATC	4 cut(s)	533, 744, 1152, 1586
MboI	GATC	4 cut(s)	531, 742, 1150, 1584
MboII	GAAGA	5 cut(s)	105, 175, 1315, 1508, 1544
MfeI	CAATTG	2 cut(s)	135, 437
MhlI	GDGCHC	1 cut(s)	146
MluCI	AATT	9 cut(s)	135, 282, 301, 437, 576, 736, 821, 839, 957
MlyI	GAGTC	2 cut(s)	22, 806
MmeI	TCCRAC	2 cut(s)	381, 1097
Mph1103I	ATGCAT	1 cut(s)	1081
MroI	TCCGGA	2 cut(s)	315, 1094
MroXI	GAANNNNTTC	1 cut(s)	1391
MseI	TTAA	8 cut(s)	503, 518, 597, 842, 858, 1041, 1065, 1269
MspA1I	CMGCKG	2 cut(s)	484, 524
MspI	CCGG	5 cut(s)	316, 416, 671, 1095, 1344
MspR9I	CCNGG	3 cut(s)	227, 559, 1344
MunI	CAATTG	2 cut(s)	135, 437
Mva1269I	GAATGC	1 cut(s)	1141
MvaI	CCWGG	2 cut(s)	227, 559
MvnI	CGCG	4 cut(s)	58, 297, 1197, 1479
MwoI	GCNNNNNNNGC	7 cut(s)	131, 195, 581, 598, 1085, 1089, 1478
NciI	CCSGG	1 cut(s)	1344
NcoI	CCATGG	1 cut(s)	788
NdeII	GATC	4 cut(s)	531, 742, 1150, 1584
NheI	GCTAGC	1 cut(s)	1019
NlaIII	CATG	8 cut(s)	39, 281, 792, 1083, 1363, 1399, 1427, 1434
NlaIV	GGNNCC	4 cut(s)	98, 143, 144, 457
NmeAIII	GCCGAG	1 cut(s)	180
NmuCI	GTSAC	3 cut(s)	1291, 1434, 1453
NsiI	ATGCAT	1 cut(s)	1081
NspI	RCATGY	2 cut(s)	39, 1083
PaeI	GCATGC	1 cut(s)	1083
PaeR7I	CTCGAG	1 cut(s)	1239
PagI	TCATGA	1 cut(s)	277
PctI	GAATGC	1 cut(s)	1141
PdmI	GAANNNNTTC	1 cut(s)	1391
PfeI	GAWTC	3 cut(s)	649, 1106, 1474
PkrI	GCNGC	9 cut(s)	13, 133, 208, 486, 526, 691, 694, 1471, 1562
PleI	GAGTC	2 cut(s)	22, 805
PpsI	GAGTC	2 cut(s)	22, 805
Ppu21I	YACGTR	1 cut(s)	1453
PpuMI	RGGWCCY	1 cut(s)	427
PsiI	TTATAA	2 cut(s)	909, 1415
Psp5II	RGGWCCY	1 cut(s)	427
Psp6I	CCWGG	2 cut(s)	225, 557
PspFI	CCCAGC	1 cut(s)	189
PspGI	CCWGG	2 cut(s)	225, 557
PspN4I	GGNNCC	4 cut(s)	98, 143, 144, 457
PspOMI	GGGCCC	1 cut(s)	142
PspPI	GGNCC	3 cut(s)	142, 143, 427
PspPPI	RGGWCCY	1 cut(s)	427
PstI	CTGCAG	1 cut(s)	529
PvuII	CAGCTG	2 cut(s)	484, 524
RsaI	GTAC	3 cut(s)	273, 985, 1265
RsaNI	GTAC	3 cut(s)	272, 984, 1264
SaqAI	TTAA	8 cut(s)	503, 518, 597, 842, 858, 1041, 1065, 1269
SatI	GCNGC	9 cut(s)	12, 132, 207, 485, 525, 690, 693, 1470, 1561
Sau3AI	GATC	4 cut(s)	531, 742, 1150, 1584
Sau96I	GGNCC	3 cut(s)	142, 143, 427
SchI	GAGTC	2 cut(s)	22, 806
ScrFI	CCNGG	3 cut(s)	227, 559, 1344
SduI	GDGCHC	1 cut(s)	146
SfaNI	GCATC	4 cut(s)	562, 907, 1018, 1353
SfcI	CTRYAG	2 cut(s)	525, 1524
Sfr274I	CTCGAG	1 cut(s)	1239
SinI	GGWCC	1 cut(s)	427
SlaI	CTCGAG	1 cut(s)	1239
SmlI	CTYRAG	1 cut(s)	1239
SmoI	CTYRAG	1 cut(s)	1239
SphI	GCATGC	1 cut(s)	1083
Sse9I	AATT	9 cut(s)	135, 282, 301, 437, 576, 736, 821, 839, 957
SsiI	CCGC	6 cut(s)	41, 58, 297, 693, 1400, 1541
SspI	AATATT	2 cut(s)	322, 386
SspMI	CTAG	3 cut(s)	344, 1020, 1493
StyD4I	CCNGG	3 cut(s)	225, 557, 1342
StyI	CCWWGG	1 cut(s)	788
TaaI	ACNGT	5 cut(s)	244, 474, 971, 1174, 1291
TaiI	ACGT	1 cut(s)	1455
TaqI	TCGA	4 cut(s)	249, 534, 825, 1240
TasI	AATT	9 cut(s)	135, 282, 301, 437, 576, 736, 821, 839, 957
TatI	WGTACW	2 cut(s)	983, 1263
TauI	GCSGC	1 cut(s)	695
TfiI	GAWTC	3 cut(s)	649, 1106, 1474
Tru1I	TTAA	8 cut(s)	503, 518, 597, 842, 858, 1041, 1065, 1269
Tru9I	TTAA	8 cut(s)	503, 518, 597, 842, 858, 1041, 1065, 1269
TscAI	CASTG	5 cut(s)	534, 976, 981, 1179, 1221
TseFI	GTSAC	3 cut(s)	1291, 1434, 1453
TseI	GCWGC	8 cut(s)	11, 131, 206, 484, 524, 689, 1469, 1560
Tsp45I	GTSAC	3 cut(s)	1291, 1434, 1453
TspDTI	ATGAA	8 cut(s)	54, 266, 294, 1098, 1338, 1384, 1419, 1551
TspGWI	ACGGA	2 cut(s)	89, 1590
TspRI	CASTG	5 cut(s)	534, 976, 981, 1179, 1221
VpaK11BI	GGWCC	1 cut(s)	427
XagI	CCTNNNNNAGG	1 cut(s)	19
XapI	RAATTY	2 cut(s)	736, 821
XceI	RCATGY	2 cut(s)	39, 1083
XcmI	CCANNNNNNNNNTGG	1 cut(s)	134
XhoI	CTCGAG	1 cut(s)	1239
XmiI	GTMKAC	1 cut(s)	1485
XmnI	GAANNNNTTC	1 cut(s)	1391
XspI	CTAG	3 cut(s)	344, 1020, 1493
Zsp2I	ATGCAT	1 cut(s)	1081

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.