FvH4_2g23210

Non-specific lipid-transfer protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012814
Physical Location & Seq
Reverse (-)
19020254 .. 19021755
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23210.t1

Sequence Viewer

Length: 606 bp
ATGGAACCCTTCAAAGCCTATCAGCCAGTTCTTATAGTAATCATTTCACTGTTAATTTCAGTTCATGGGCAGATTAGTACACCATGCACAACCACAATGCTTAGCACCTTCACTCCATGCTTGAATTATATTACTGGGAGTACGAGCAACGGCTCATCACCAACAGGGGACTGTTGCGGTGCGCTGAAGTCGTTAATGGGGACGGGCATGGACTGCGCTTGCCTATTAGTAACCTCCAATGTTCCTGTCCAACTTCCCATCAACAGAACCCTTGCTCTCTCTCTCCCTAAAGCATGTAAAATGGGGGGTGTGCCACTACAATGCAAAGCCTCTGCTAGTCCTTTACCTGCCCCAGGTCCTTCCTTGCTAGGACCAACTCCTTCCCCAACATCTGATTCTCCTTTTAGTCCAAGAGCTTCTAAAGCAGTAGCAACAGCTCCTACACCAGAATCTGAAGCAGACTTTCCATCAGATGAACCTGAAGCTCCGACAACTGCGATCAATCGACCAGTGCTGACGCCATCTGCAGCCTCTAGTCTATCATTTGTTTCCCCACAGCCTCTGCTACTCGTAGTTTTAGGAATCATGGTTTATAAATCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

202

Amino Acids

20.65

Weight (kDa)

6.53

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 15 - 108 2.2e-20 Probable lipid transfer
Tryp_alpha_amyl PF00234 29 - 102 3.5e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012814)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G48140 AT2G48140
fragaria_vesca FvH4_2g23210
malus_domestica MD03G1261400.v1.1 MD11G1281900.v1.1 MD11G1282200.v1.1
prunus_persica Prupe.8G237800_v2.0.a1
pyrus_communis pycom03g20940 pycom11g24990
rosa_chinensis RchiOBHm_Chr6g0290481
rosa_laevigata RLG00000012211
rosa_roxburghii Rroxscaffold_7G00177020
rosa_rugosa Rorug06G0212900 Rorug06G0212900
rosa_samantha Rh6AG323300 Rh6BG330500 Rh6DG323700
rosa_wichuraiana Rw6G028030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 594
Acc36I ACCTGC 1 cut(s) 355
AciI CCGC 1 cut(s) 177
AcuI CTGAAG 3 cut(s) 206, 474, 501
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 2 cut(s) 79, 142
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 2 cut(s) 13, 124
AjnI CCWGG 1 cut(s) 352
AjuI GAANNNNNNNTTGG 2 cut(s) 379, 411
AluBI AGCT 3 cut(s) 416, 437, 485
AluI AGCT 3 cut(s) 416, 437, 485
AlwNI CAGNNNCTG 2 cut(s) 452, 562
ApeKI GCWGC 1 cut(s) 527
AspLEI GCGC 2 cut(s) 184, 218
AspS9I GGNCC 2 cut(s) 356, 371
AsuHPI GGTGA 1 cut(s) 150
AvaII GGWCC 2 cut(s) 356, 371
BbvI GCAGC 1 cut(s) 539
BccI CCATC 3 cut(s) 266, 475, 529
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 1 cut(s) 354
BfaI CTAG 4 cut(s) 336, 368, 534, 604
BfmI CTRYAG 1 cut(s) 525
BfuAI ACCTGC 1 cut(s) 355
BisI GCNGC 1 cut(s) 528
BlpI GCTNAGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 529
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 2 cut(s) 356, 371
BmgT120I GGNCC 2 cut(s) 356, 371
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 354
BmrI ACTGGG 1 cut(s) 144
BmuI ACTGGG 1 cut(s) 144
Bpu1102I GCTNAGC 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 518
BsaJI CCNNGG 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 26, 139, 509
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 353
BseNI ACTGG 3 cut(s) 26, 139, 509
BseXI GCAGC 1 cut(s) 539
BslFI GGGAC 2 cut(s) 182, 214
BslI CCNNNNNNNGG 1 cut(s) 353
BsmFI GGGAC 2 cut(s) 182, 214
Bsp143I GATC 1 cut(s) 498
Bsp1720I GCTNAGC 1 cut(s) 101
BspACI CCGC 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 355
BsrI ACTGG 3 cut(s) 26, 139, 509
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 1 cut(s) 498
BssNI GRCGYC 1 cut(s) 518
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 51, 173
BstACI GRCGYC 1 cut(s) 518
BstAPI GCANNNNNTGC 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 1 cut(s) 101
BstENI CCTNNNNNAGG 1 cut(s) 351
BstHHI GCGC 2 cut(s) 184, 218
BstKTI GATC 1 cut(s) 501
BstMBI GATC 1 cut(s) 498
BstMWI GCNNNNNNNGC 2 cut(s) 213, 422
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 352
BstSFI CTRYAG 1 cut(s) 525
BstV1I GCAGC 1 cut(s) 539
BtsIMutI CAGTG 2 cut(s) 47, 516
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 1 cut(s) 220
CaiI CAGNNNCTG 2 cut(s) 452, 562
CfoI GCGC 2 cut(s) 184, 218
Cfr13I GGNCC 2 cut(s) 356, 371
CseI GACGC 1 cut(s) 526
Csp6I GTAC 2 cut(s) 78, 141
CviAII CATG 6 cut(s) 65, 84, 117, 208, 294, 586
CviJI RGCY 9 cut(s) 17, 25, 153, 329, 416, 437, 485, 530, 559
CviKI_1 RGCY 9 cut(s) 17, 25, 153, 329, 416, 437, 485, 530, 559
CviQI GTAC 2 cut(s) 78, 141
DdeI CTNAG 1 cut(s) 101
DpnI GATC 1 cut(s) 500
DpnII GATC 1 cut(s) 498
Eco47I GGWCC 2 cut(s) 356, 371
Eco57I CTGAAG 3 cut(s) 206, 474, 501
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 352
FaeI CATG 6 cut(s) 68, 87, 120, 211, 297, 589
FaiI YATR 9 cut(s) 35, 66, 85, 118, 129, 209, 295, 587, 594
FaqI GGGAC 2 cut(s) 182, 214
FatI CATG 6 cut(s) 64, 83, 116, 207, 293, 585
Fnu4HI GCNGC 1 cut(s) 528
Fsp4HI GCNGC 1 cut(s) 528
FspBI CTAG 4 cut(s) 336, 368, 534, 604
GlaI GCGC 2 cut(s) 183, 217
GluI GCNGC 1 cut(s) 528
HgaI GACGC 1 cut(s) 526
HhaI GCGC 2 cut(s) 184, 218
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 6 cut(s) 68, 87, 120, 211, 297, 589
Hin6I GCGC 2 cut(s) 182, 216
HinP1I GCGC 2 cut(s) 182, 216
HinfI GANTC 3 cut(s) 395, 449, 582
HphI GGTGA 1 cut(s) 150
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 4 cut(s) 394, 454, 472, 489
Hpy8I GTNNAC 1 cut(s) 80
HpyAV CCTTC 4 cut(s) 19, 118, 369, 390
HpyCH4III ACNGT 2 cut(s) 51, 173
HpyCH4V TGCA 3 cut(s) 87, 324, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 213, 422
HpyF3I CTNAG 1 cut(s) 101
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 6 cut(s) 68, 87, 120, 211, 297, 589
HspAI GCGC 2 cut(s) 182, 216
Kzo9I GATC 1 cut(s) 498
LmnI GCTCC 2 cut(s) 442, 490
Lsp1109I GCAGC 1 cut(s) 539
MaeI CTAG 4 cut(s) 336, 368, 534, 604
MaeIII GTNAC 1 cut(s) 229
MalI GATC 1 cut(s) 500
MboI GATC 1 cut(s) 498
MluCI AATT 2 cut(s) 54, 124
MmeI TCCRAC 2 cut(s) 274, 512
MnlI CCTC 4 cut(s) 244, 340, 541, 570
MseI TTAA 2 cut(s) 53, 194
MslI CAYNNNNRTG 1 cut(s) 319
MspR9I CCNGG 1 cut(s) 354
MvaI CCWGG 1 cut(s) 354
MwoI GCNNNNNNNGC 2 cut(s) 213, 422
NdeII GATC 1 cut(s) 498
NlaIII CATG 6 cut(s) 68, 87, 120, 211, 297, 589
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 297
PfeI GAWTC 3 cut(s) 395, 449, 582
PkrI GCNGC 1 cut(s) 529
PpuMI RGGWCCY 1 cut(s) 356
PsiI TTATAA 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 356
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 356, 371
PspPPI RGGWCCY 1 cut(s) 356
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 2 cut(s) 452, 562
RsaI GTAC 2 cut(s) 79, 142
RsaNI GTAC 2 cut(s) 78, 141
RseI CAYNNNNRTG 1 cut(s) 319
SaqAI TTAA 2 cut(s) 53, 194
SatI GCNGC 1 cut(s) 528
Sau3AI GATC 1 cut(s) 498
Sau96I GGNCC 2 cut(s) 356, 371
ScrFI CCNGG 1 cut(s) 354
SetI ASST 8 cut(s) 110, 236, 349, 358, 418, 439, 481, 487
SfcI CTRYAG 1 cut(s) 525
SinI GGWCC 2 cut(s) 356, 371
SmiMI CAYNNNNRTG 1 cut(s) 319
Sse9I AATT 2 cut(s) 54, 124
SsiI CCGC 1 cut(s) 177
SspMI CTAG 4 cut(s) 336, 368, 534, 604
StyD4I CCNGG 1 cut(s) 352
TaaI ACNGT 2 cut(s) 51, 173
TaqI TCGA 1 cut(s) 505
TasI AATT 2 cut(s) 54, 124
TatI WGTACW 1 cut(s) 77
TfiI GAWTC 3 cut(s) 395, 449, 582
Tru1I TTAA 2 cut(s) 53, 194
Tru9I TTAA 2 cut(s) 53, 194
TscAI CASTG 2 cut(s) 54, 516
TseI GCWGC 1 cut(s) 527
TspDTI ATGAA 2 cut(s) 53, 489
TspRI CASTG 2 cut(s) 54, 516
VpaK11BI GGWCC 2 cut(s) 356, 371
XagI CCTNNNNNAGG 1 cut(s) 351
XceI RCATGY 1 cut(s) 297
XspI CTAG 4 cut(s) 336, 368, 534, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.