Rroxscaffold_7G00177020

Non-specific lipid-transfer protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012814
Physical Location & Seq
Forward (+)
16585778 .. 16587857
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00177020.1

Sequence Viewer

Length: 600 bp
ATGGAACCCTGCAAGCCCTTTCTTTCAGTTCTTGTAGTACTCATTTCACTGCTAATTTCAGTTAATGGGCAGATTAGTACACCATGCACAGCCTCAAAGCTTAGCACCTTAACTCCATGCTTGAATTATATAACTGGGAGTACCAACAATGGCTCATCGCCAACAGGGGACTGTTGCTATGCCCTAAAGATCAGCATGGACTGCGCTTGCCTTTTAGTAACCGCCAATGTTCCAATCCAACTGCCCATTAACAGAACCCTTGCTCTCTCTCTTCCTAAAGCATGTAAAATGGGGAGTGTGCCACTACAATGCAAAGCTTCTGCTACTCCTTTAGCTGCCCCAGGTCCTTCCTTACTAGGACCAACTATTTCCCCCACAGCTGCTGATTCTCCTTTAAGTCCAAGAGCAGCTTCTAAAGCAGTAGCAACAGGTCCTGCACCACAATCTGAACCAGAATCTCCATCAGTTGAACCTGAAGCTCCGACAACCGCGATCAATCGACCACTGCTGACGCCAACTGCAGCCTCTAGTCTATCTTATGTTTCTCCACAACCTCTGCTACTCATAGTTTTAGGAATCATGGTTTATAATTCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

199

Amino Acids

20.35

Weight (kDa)

8.07

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 13 - 104 9.3e-17 Probable lipid transfer
Tryp_alpha_amyl PF00234 34 - 104 2e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012814)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G48140 AT2G48140
fragaria_vesca FvH4_2g23210
malus_domestica MD03G1261400.v1.1 MD11G1281900.v1.1 MD11G1282200.v1.1
prunus_persica Prupe.8G237800_v2.0.a1
pyrus_communis pycom03g20940 pycom11g24990
rosa_chinensis RchiOBHm_Chr6g0290481
rosa_laevigata RLG00000012211
rosa_roxburghii Rroxscaffold_7G00177020
rosa_rugosa Rorug06G0212900 Rorug06G0212900
rosa_samantha Rh6AG323300 Rh6BG330500 Rh6DG323700
rosa_wichuraiana Rw6G028030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AccII CGCG 1 cut(s) 491
AciI CCGC 2 cut(s) 222, 489
AcuI CTGAAG 1 cut(s) 495
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 3 cut(s) 39, 79, 142
AgsI TTSAA 2 cut(s) 124, 470
AjnI CCWGG 1 cut(s) 340
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AluBI AGCT 6 cut(s) 100, 317, 335, 380, 410, 479
AluI AGCT 6 cut(s) 100, 317, 335, 380, 410, 479
AlwNI CAGNNNCTG 2 cut(s) 383, 434
ApeKI GCWGC 4 cut(s) 335, 380, 407, 521
AspLEI GCGC 1 cut(s) 206
AspS9I GGNCC 3 cut(s) 344, 359, 431
AvaII GGWCC 3 cut(s) 344, 359, 431
BbvI GCAGC 4 cut(s) 322, 367, 419, 533
BccI CCATC 1 cut(s) 469
BciT130I CCWGG 1 cut(s) 342
BfaI CTAG 3 cut(s) 356, 528, 598
BfmI CTRYAG 1 cut(s) 519
BisI GCNGC 4 cut(s) 336, 381, 408, 522
BlpI GCTNAGC 1 cut(s) 101
BlsI GCNGC 4 cut(s) 337, 382, 409, 523
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 3 cut(s) 344, 359, 431
BmgT120I GGNCC 3 cut(s) 344, 359, 431
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 342
BmrI ACTGGG 1 cut(s) 144
BmuI ACTGGG 1 cut(s) 144
Bpu1102I GCTNAGC 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 512
BsaJI CCNNGG 1 cut(s) 340
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 340
BseNI ACTGG 1 cut(s) 139
BseXI GCAGC 4 cut(s) 322, 367, 419, 533
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 1 cut(s) 491
BslFI GGGAC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 182
Bsp143I GATC 2 cut(s) 189, 492
Bsp1720I GCTNAGC 1 cut(s) 101
BspACI CCGC 2 cut(s) 222, 489
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 523
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 2 cut(s) 189, 492
BssNI GRCGYC 1 cut(s) 512
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 512
BstAPI GCANNNNNTGC 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 14, 208
BstDEI CTNAG 1 cut(s) 101
BstFNI CGCG 1 cut(s) 491
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 2 cut(s) 192, 495
BstMBI GATC 2 cut(s) 189, 492
BstMWI GCNNNNNNNGC 2 cut(s) 201, 416
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 4 cut(s) 322, 367, 419, 533
BtgZI GCGATG 1 cut(s) 141
BtsI GCAGTG 2 cut(s) 47, 503
BtsIMutI CAGTG 2 cut(s) 47, 503
Cac8I GCNNGC 2 cut(s) 14, 208
CaiI CAGNNNCTG 2 cut(s) 383, 434
CfoI GCGC 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 344, 359, 431
CseI GACGC 1 cut(s) 520
Csp6I GTAC 3 cut(s) 38, 78, 141
CviAII CATG 5 cut(s) 84, 117, 196, 282, 580
CviQI GTAC 3 cut(s) 38, 78, 141
DdeI CTNAG 1 cut(s) 101
DpnI GATC 2 cut(s) 191, 494
DpnII GATC 2 cut(s) 189, 492
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 344, 359, 431
Eco57I CTGAAG 1 cut(s) 495
EcoO109I RGGNCCY 2 cut(s) 344, 431
EcoRII CCWGG 1 cut(s) 340
FaeI CATG 5 cut(s) 87, 120, 199, 285, 583
FalI AAGNNNNNCTT 2 cut(s) 394, 426
FaqI GGGAC 1 cut(s) 182
FatI CATG 5 cut(s) 83, 116, 195, 281, 579
Fnu4HI GCNGC 4 cut(s) 336, 381, 408, 522
Fsp4HI GCNGC 4 cut(s) 336, 381, 408, 522
FspBI CTAG 3 cut(s) 356, 528, 598
GlaI GCGC 1 cut(s) 205
GluI GCNGC 4 cut(s) 336, 381, 408, 522
HgaI GACGC 1 cut(s) 520
HhaI GCGC 1 cut(s) 206
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 5 cut(s) 87, 120, 199, 285, 583
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HindIII AAGCTT 2 cut(s) 98, 315
HinfI GANTC 3 cut(s) 386, 455, 576
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 2 cut(s) 448, 483
Hpy8I GTNNAC 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 357
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 5 cut(s) 12, 87, 312, 437, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 416
HpyF3I CTNAG 1 cut(s) 101
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 5 cut(s) 87, 120, 199, 285, 583
HspAI GCGC 1 cut(s) 204
Kzo9I GATC 2 cut(s) 189, 492
LmnI GCTCC 1 cut(s) 484
LpnPI CCDG 9 cut(s) 22, 120, 150, 327, 354, 414, 447, 465, 486
Lsp1109I GCAGC 4 cut(s) 322, 367, 419, 533
MaeI CTAG 3 cut(s) 356, 528, 598
MaeIII GTNAC 1 cut(s) 217
MalI GATC 2 cut(s) 191, 494
MboI GATC 2 cut(s) 189, 492
MboII GAAGA 1 cut(s) 263
MluCI AATT 3 cut(s) 54, 124, 589
MmeI TCCRAC 2 cut(s) 262, 506
MnlI CCTC 3 cut(s) 103, 535, 564
MseI TTAA 4 cut(s) 63, 110, 249, 395
MslI CAYNNNNRTG 1 cut(s) 307
MspA1I CMGCKG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 342
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 2 cut(s) 201, 416
NdeII GATC 2 cut(s) 189, 492
NlaIII CATG 5 cut(s) 87, 120, 199, 285, 583
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 285
PfeI GAWTC 3 cut(s) 386, 455, 576
PkrI GCNGC 4 cut(s) 337, 382, 409, 523
PpuMI RGGWCCY 2 cut(s) 344, 431
PsiI TTATAA 1 cut(s) 588
Psp5II RGGWCCY 2 cut(s) 344, 431
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 344, 359, 431
PspPPI RGGWCCY 2 cut(s) 344, 431
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 2 cut(s) 383, 434
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 3 cut(s) 39, 79, 142
RsaNI GTAC 3 cut(s) 38, 78, 141
RseI CAYNNNNRTG 1 cut(s) 307
SaqAI TTAA 4 cut(s) 63, 110, 249, 395
SatI GCNGC 4 cut(s) 336, 381, 408, 522
Sau3AI GATC 2 cut(s) 189, 492
Sau96I GGNCC 3 cut(s) 344, 359, 431
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 342
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 3 cut(s) 344, 359, 431
SmiMI CAYNNNNRTG 1 cut(s) 307
Sse9I AATT 3 cut(s) 54, 124, 589
SsiI CCGC 2 cut(s) 222, 489
SspMI CTAG 3 cut(s) 356, 528, 598
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 1 cut(s) 499
TasI AATT 3 cut(s) 54, 124, 589
TatI WGTACW 2 cut(s) 37, 77
TfiI GAWTC 3 cut(s) 386, 455, 576
Tru1I TTAA 4 cut(s) 63, 110, 249, 395
Tru9I TTAA 4 cut(s) 63, 110, 249, 395
TscAI CASTG 2 cut(s) 54, 510
TseI GCWGC 4 cut(s) 335, 380, 407, 521
TspRI CASTG 2 cut(s) 54, 510
VpaK11BI GGWCC 3 cut(s) 344, 359, 431
XceI RCATGY 1 cut(s) 285
XspI CTAG 3 cut(s) 356, 528, 598
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.