Rw6G028030

Non-specific lipid-transfer protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012814
Physical Location & Seq
Reverse (-)
51958557 .. 51959491
935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G028030.1

Sequence Viewer

Length: 597 bp
ATGGAACCCTGCAAGCCCTTTCCTTCAGTTCTTGTAGTACTCATTTCACTGCTAATTTCAGTTAATGGGCAGATTAGTACACCATGCACAGCCTCAAATCTTGGCACCTTAACTCCGTGCTTGAATTATATAACTGGGAGTACCAACAATGGCTCATCGCCAACAGGGGACTGTTGCTATGCCCTAAAGACCAGCATGGACTGCGCTTGCCTTTTAGTAACCGCCAATGTTCCAATCCAACTGCCCATTAACAGAACCCTTGCTCTCTCTCTTCCTAAAGCATGTAAAATGGGGAGTGTGCCACTACAATGCAAAGCTTCTGCTACTCCTTTAGCTGCCCCAGGTCCTTCCTTACTAGGACCAACCATTTCCCCCACAACTGCTGATTCTCCTTTAAGTCCAAGAGCTTCTAAAGCAGTAGCAACAGGTCCTGCACCACAATCTGAAGCAGAATCTCCATCAGTTGAACCTGAAGCTCCGACAACTGCGATCAATCGACCACTGCTGACGCCAACTGCAGCCTCTAGTCTATCTTATGTTTCTCCACAACCTCTGCTACTCATAGTTTTAGGAATCATGGTTTATAAATCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

198

Amino Acids

20.22

Weight (kDa)

8.07

Isoelectric Point (pI)

53.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 13 - 104 4.7e-17 Probable lipid transfer
Tryp_alpha_amyl PF00234 34 - 104 2e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012814)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G48140 AT2G48140
fragaria_vesca FvH4_2g23210
malus_domestica MD03G1261400.v1.1 MD11G1281900.v1.1 MD11G1282200.v1.1
prunus_persica Prupe.8G237800_v2.0.a1
pyrus_communis pycom03g20940 pycom11g24990
rosa_chinensis RchiOBHm_Chr6g0290481
rosa_laevigata RLG00000012211
rosa_roxburghii Rroxscaffold_7G00177020
rosa_rugosa Rorug06G0212900 Rorug06G0212900
rosa_samantha Rh6AG323300 Rh6BG330500 Rh6DG323700
rosa_wichuraiana Rw6G028030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AccB1I GGYRCC 1 cut(s) 104
AciI CCGC 1 cut(s) 222
AcuI CTGAAG 3 cut(s) 9, 465, 492
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 3 cut(s) 39, 79, 142
AgsI TTSAA 2 cut(s) 124, 467
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 4 cut(s) 317, 335, 407, 476
AluI AGCT 4 cut(s) 317, 335, 407, 476
AlwNI CAGNNNCTG 1 cut(s) 431
ApeKI GCWGC 2 cut(s) 335, 518
AspLEI GCGC 1 cut(s) 206
AspS9I GGNCC 3 cut(s) 344, 359, 428
AvaII GGWCC 3 cut(s) 344, 359, 428
BanI GGYRCC 1 cut(s) 104
BbvI GCAGC 2 cut(s) 322, 530
BccI CCATC 1 cut(s) 466
BciT130I CCWGG 1 cut(s) 342
BfaI CTAG 3 cut(s) 356, 525, 595
BfmI CTRYAG 1 cut(s) 516
BisI GCNGC 2 cut(s) 336, 519
BlsI GCNGC 2 cut(s) 337, 520
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 3 cut(s) 344, 359, 428
BmgT120I GGNCC 3 cut(s) 344, 359, 428
BmiI GGNNCC 2 cut(s) 6, 106
BmrFI CCNGG 1 cut(s) 342
BmrI ACTGGG 1 cut(s) 144
BmuI ACTGGG 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 340
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 340
BseNI ACTGG 1 cut(s) 139
BseXI GCAGC 2 cut(s) 322, 530
BsgI GTGCAG 1 cut(s) 417
BshNI GGYRCC 1 cut(s) 104
BslFI GGGAC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 182
Bsp143I GATC 1 cut(s) 489
BspACI CCGC 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 6, 106
BspMAI CTGCAG 1 cut(s) 520
BspT107I GGYRCC 1 cut(s) 104
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 1 cut(s) 489
BssNI GRCGYC 1 cut(s) 509
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 509
BstAPI GCANNNNNTGC 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 14, 208
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 1 cut(s) 492
BstMBI GATC 1 cut(s) 489
BstMWI GCNNNNNNNGC 2 cut(s) 201, 413
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 516
BstV1I GCAGC 2 cut(s) 322, 530
BtgZI GCGATG 1 cut(s) 141
BtsI GCAGTG 2 cut(s) 47, 500
BtsIMutI CAGTG 2 cut(s) 47, 500
Cac8I GCNNGC 2 cut(s) 14, 208
CaiI CAGNNNCTG 1 cut(s) 431
CfoI GCGC 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 344, 359, 428
CseI GACGC 1 cut(s) 517
Csp6I GTAC 3 cut(s) 38, 78, 141
CviAII CATG 4 cut(s) 84, 196, 282, 577
CviJI RGCY 8 cut(s) 16, 92, 153, 317, 335, 407, 476, 521
CviKI_1 RGCY 8 cut(s) 16, 92, 153, 317, 335, 407, 476, 521
CviQI GTAC 3 cut(s) 38, 78, 141
DpnI GATC 1 cut(s) 491
DpnII GATC 1 cut(s) 489
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 344, 359, 428
Eco57I CTGAAG 3 cut(s) 9, 465, 492
EcoO109I RGGNCCY 2 cut(s) 344, 428
EcoRII CCWGG 1 cut(s) 340
FaeI CATG 4 cut(s) 87, 199, 285, 580
FaqI GGGAC 1 cut(s) 182
FatI CATG 4 cut(s) 83, 195, 281, 576
Fnu4HI GCNGC 2 cut(s) 336, 519
Fsp4HI GCNGC 2 cut(s) 336, 519
FspBI CTAG 3 cut(s) 356, 525, 595
GlaI GCGC 1 cut(s) 205
GluI GCNGC 2 cut(s) 336, 519
HgaI GACGC 1 cut(s) 517
HhaI GCGC 1 cut(s) 206
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 4 cut(s) 87, 199, 285, 580
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 3 cut(s) 386, 452, 573
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 2 cut(s) 445, 480
Hpy8I GTNNAC 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 33, 357
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 5 cut(s) 12, 87, 312, 434, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 413
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 4 cut(s) 87, 199, 285, 580
HspAI GCGC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 489
LmnI GCTCC 1 cut(s) 481
LpnPI CCDG 9 cut(s) 22, 120, 150, 205, 327, 354, 411, 444, 483
Lsp1109I GCAGC 2 cut(s) 322, 530
MaeI CTAG 3 cut(s) 356, 525, 595
MaeIII GTNAC 1 cut(s) 217
MalI GATC 1 cut(s) 491
MboI GATC 1 cut(s) 489
MboII GAAGA 1 cut(s) 263
MluCI AATT 2 cut(s) 54, 124
MmeI TCCRAC 2 cut(s) 262, 503
MnlI CCTC 3 cut(s) 103, 532, 561
MseI TTAA 4 cut(s) 63, 110, 249, 395
MslI CAYNNNNRTG 1 cut(s) 307
MspR9I CCNGG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 2 cut(s) 201, 413
NdeII GATC 1 cut(s) 489
NlaIII CATG 4 cut(s) 87, 199, 285, 580
NlaIV GGNNCC 2 cut(s) 6, 106
NspI RCATGY 1 cut(s) 285
PfeI GAWTC 3 cut(s) 386, 452, 573
PkrI GCNGC 2 cut(s) 337, 520
PpuMI RGGWCCY 2 cut(s) 344, 428
PsiI TTATAA 1 cut(s) 585
Psp5II RGGWCCY 2 cut(s) 344, 428
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 6, 106
PspPI GGNCC 3 cut(s) 344, 359, 428
PspPPI RGGWCCY 2 cut(s) 344, 428
PstI CTGCAG 1 cut(s) 520
PstNI CAGNNNCTG 1 cut(s) 431
RsaI GTAC 3 cut(s) 39, 79, 142
RsaNI GTAC 3 cut(s) 38, 78, 141
RseI CAYNNNNRTG 1 cut(s) 307
SaqAI TTAA 4 cut(s) 63, 110, 249, 395
SatI GCNGC 2 cut(s) 336, 519
Sau3AI GATC 1 cut(s) 489
Sau96I GGNCC 3 cut(s) 344, 359, 428
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 342
SetI ASST 9 cut(s) 110, 319, 337, 346, 409, 430, 472, 478, 553
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 3 cut(s) 344, 359, 428
SmiMI CAYNNNNRTG 1 cut(s) 307
Sse9I AATT 2 cut(s) 54, 124
SsiI CCGC 1 cut(s) 222
SspMI CTAG 3 cut(s) 356, 525, 595
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 1 cut(s) 496
TasI AATT 2 cut(s) 54, 124
TatI WGTACW 2 cut(s) 37, 77
TfiI GAWTC 3 cut(s) 386, 452, 573
Tru1I TTAA 4 cut(s) 63, 110, 249, 395
Tru9I TTAA 4 cut(s) 63, 110, 249, 395
TscAI CASTG 2 cut(s) 54, 507
TseI GCWGC 2 cut(s) 335, 518
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 2 cut(s) 54, 507
VpaK11BI GGWCC 3 cut(s) 344, 359, 428
XceI RCATGY 1 cut(s) 285
XspI CTAG 3 cut(s) 356, 525, 595
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.