MD03G1261400.v1.1

Non-specific lipid-transfer protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012814
Physical Location & Seq
Reverse (-)
34786179 .. 34787878
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1261400.v1.1.491

Sequence Viewer

Length: 633 bp
ATGGAATCATTCAAGGATTGTCGATTAAGTCTAATCCCAATTACCCTCCTAATCATTTCACTAAGATTGGTTAATGGGCAGATTAGTACACCATGCACAACCTCAATGATAAGCAGCGTCACTCCATGCGCAAATTACATCACCGGGAGTACCAGCAACGGCGGGTCACCGACAGCCGGATGTTGCAGTTCGTTAAAGTCGTTAATGGGCACCGGCATGGACTGTGCTTGTCTTCTTATAACTGGTGGTGTCCCTGTCCAACTGCCCATTAACCGAACCCTCGCGCTTTCTCTTCCTCGAGCTTGTAAGATGGGAGGCGTCCCAGTGCAATGCAAGGCCTCTGGTAGTCCTTTACCTGCTCCAGGTCCTTCGTTACTAGGGCCAACTCCTCCACCAACAGTTTCTTCAAGTCCAAGAGCTTCTAAGGCAGTTGCATCAGGTCCTGCACCAGAATCTGGAACATCTGATGATCTGCAGCCAGCATCTCCATCAGATGAATCAGAAGCTCCAACACAAAGTACCCAAGGAATCGGACGACCACAGCTGACCCCATCAGCATCGAGTTTATCTTATGTTTCCCCACCATCTGTCCTACTGATAATGTTGGGAATTATGGTTTTCAATTCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

211

Amino Acids

21.21

Weight (kDa)

8.09

Isoelectric Point (pI)

56.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 26 - 111 1.6e-19 Probable lipid transfer
Tryp_alpha_amyl PF00234 36 - 111 1.5e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012814)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G48140 AT2G48140
fragaria_vesca FvH4_2g23210
malus_domestica MD03G1261400.v1.1 MD11G1281900.v1.1 MD11G1282200.v1.1
prunus_persica Prupe.8G237800_v2.0.a1
pyrus_communis pycom03g20940 pycom11g24990
rosa_chinensis RchiOBHm_Chr6g0290481
rosa_laevigata RLG00000012211
rosa_roxburghii Rroxscaffold_7G00177020
rosa_rugosa Rorug06G0212900 Rorug06G0212900
rosa_samantha Rh6AG323300 Rh6BG330500 Rh6DG323700
rosa_wichuraiana Rw6G028030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 239
Acc16I TGCGCA 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 364
AccB1I GGYRCC 1 cut(s) 209
AccB7I CCANNNNNTGG 1 cut(s) 455
AccII CGCG 1 cut(s) 284
AciI CCGC 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 3 cut(s) 88, 151, 520
AfiI CCNNNNNNNGG 3 cut(s) 176, 362, 455
AgsI TTSAA 3 cut(s) 13, 408, 622
AjnI CCWGG 1 cut(s) 361
AjuI GAANNNNNNNTTGG 2 cut(s) 388, 420
AluBI AGCT 4 cut(s) 302, 419, 506, 544
AluI AGCT 4 cut(s) 302, 419, 506, 544
AlwNI CAGNNNCTG 2 cut(s) 443, 455
Ama87I CYCGRG 1 cut(s) 297
AoxI GGCC 2 cut(s) 336, 380
ApeKI GCWGC 2 cut(s) 114, 475
AspLEI GCGC 2 cut(s) 131, 286
AspS9I GGNCC 3 cut(s) 365, 380, 440
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 2 cut(s) 133, 159
AvaI CYCGRG 1 cut(s) 297
AvaII GGWCC 2 cut(s) 365, 440
BaeGI GKGCMC 1 cut(s) 212
BanI GGYRCC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 224
BbvI GCAGC 2 cut(s) 126, 487
BccI CCATC 4 cut(s) 304, 496, 559, 592
BceAI ACGGC 1 cut(s) 175
BciT130I CCWGG 1 cut(s) 363
BcnI CCSGG 1 cut(s) 145
BfaI CTAG 2 cut(s) 377, 631
BfmI CTRYAG 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 364
BisI GCNGC 2 cut(s) 115, 476
BlsI GCNGC 2 cut(s) 116, 477
Bme1390I CCNGG 2 cut(s) 145, 363
Bme18I GGWCC 2 cut(s) 365, 440
BmeT110I CYCGRG 1 cut(s) 297
BmgT120I GGNCC 3 cut(s) 365, 380, 440
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 145, 363
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 3 cut(s) 443, 491, 566
BmuI ACTGGG 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 145
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 523
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 362, 455
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 247, 323
Bse3DI GCAATG 1 cut(s) 335
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 523
BseGI GGATG 1 cut(s) 185
BseLI CCNNNNNNNGG 3 cut(s) 176, 362, 455
BseMI GCAATG 1 cut(s) 335
BseNI ACTGG 2 cut(s) 247, 323
BseRI GAGGAG 1 cut(s) 378
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 2 cut(s) 126, 487
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 2 cut(s) 338, 382
BshNI GGYRCC 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 3 cut(s) 144, 177, 213
BslFI GGGAC 2 cut(s) 236, 305
BslI CCNNNNNNNGG 3 cut(s) 176, 362, 455
BsmFI GGGAC 2 cut(s) 236, 305
BsnI GGCC 2 cut(s) 338, 382
BsoBI CYCGRG 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 1 cut(s) 469
BspACI CCGC 1 cut(s) 162
BspANI GGCC 2 cut(s) 338, 382
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 335
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 2 cut(s) 247, 323
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 523
BssMI GATC 1 cut(s) 469
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 523
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 224, 400
Bst6I CTCTTC 1 cut(s) 297
BstACI GRCGYC 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 2 cut(s) 62, 423
BstEII GGTNACC 1 cut(s) 165
BstENI CCTNNNNNAGG 1 cut(s) 360
BstF5I GGATG 1 cut(s) 185
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 2 cut(s) 131, 286
BstKTI GATC 1 cut(s) 472
BstMBI GATC 1 cut(s) 469
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 1 cut(s) 363
BstPI GGTNACC 1 cut(s) 165
BstSCI CCNGG 2 cut(s) 143, 361
BstSFI CTRYAG 1 cut(s) 473
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 2 cut(s) 126, 487
BstV2I GAAGAC 1 cut(s) 224
BsuRI GGCC 2 cut(s) 338, 382
BtsCI GGATG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 330
BveI ACCTGC 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 480
CaiI CAGNNNCTG 2 cut(s) 443, 455
CfoI GCGC 2 cut(s) 131, 286
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 3 cut(s) 365, 380, 440
CseI GACGC 2 cut(s) 106, 307
Csp6I GTAC 3 cut(s) 87, 150, 519
CviAII CATG 3 cut(s) 93, 126, 217
CviJI RGCY 8 cut(s) 176, 302, 338, 382, 419, 478, 506, 544
CviKI_1 RGCY 8 cut(s) 176, 302, 338, 382, 419, 478, 506, 544
CviQI GTAC 3 cut(s) 87, 150, 519
DdeI CTNAG 2 cut(s) 62, 423
DpnI GATC 1 cut(s) 471
DpnII GATC 1 cut(s) 469
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 523
Eco147I AGGCCT 1 cut(s) 338
Eco47I GGWCC 2 cut(s) 365, 440
Eco88I CYCGRG 1 cut(s) 297
Eco91I GGTNACC 1 cut(s) 165
EcoNI CCTNNNNNAGG 1 cut(s) 360
EcoO109I RGGNCCY 2 cut(s) 365, 440
EcoO65I GGTNACC 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 523
FaeI CATG 3 cut(s) 96, 129, 220
FaiI YATR 6 cut(s) 94, 127, 218, 239, 573, 614
FaqI GGGAC 2 cut(s) 236, 305
FatI CATG 3 cut(s) 92, 125, 216
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 2 cut(s) 115, 476
FokI GGATG 1 cut(s) 192
Fsp4HI GCNGC 2 cut(s) 115, 476
FspBI CTAG 2 cut(s) 377, 631
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 2 cut(s) 130, 285
GluI GCNGC 2 cut(s) 115, 476
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 2 cut(s) 338, 382
HapII CCGG 3 cut(s) 144, 177, 213
HgaI GACGC 2 cut(s) 106, 307
HhaI GCGC 2 cut(s) 131, 286
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 3 cut(s) 96, 129, 220
Hin6I GCGC 2 cut(s) 129, 284
HinP1I GCGC 2 cut(s) 129, 284
HinfI GANTC 4 cut(s) 5, 452, 497, 528
HpaII CCGG 3 cut(s) 144, 177, 213
HphI GGTGA 2 cut(s) 133, 159
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 4 cut(s) 466, 493, 502, 533
Hpy188III TCNNGA 1 cut(s) 456
Hpy8I GTNNAC 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 378
HpyCH4III ACNGT 2 cut(s) 224, 400
HpyCH4V TGCA 7 cut(s) 96, 186, 328, 333, 434, 446, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpyF3I CTNAG 2 cut(s) 62, 423
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 3 cut(s) 96, 129, 220
HspAI GCGC 2 cut(s) 129, 284
Kzo9I GATC 1 cut(s) 469
LmnI GCTCC 2 cut(s) 364, 511
Lsp1109I GCAGC 2 cut(s) 126, 487
LweI GCATC 3 cut(s) 443, 491, 566
MaeI CTAG 2 cut(s) 377, 631
MaeIII GTNAC 3 cut(s) 118, 165, 372
MalI GATC 1 cut(s) 471
MboI GATC 1 cut(s) 469
MboII GAAGA 3 cut(s) 224, 284, 396
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 4 cut(s) 39, 133, 609, 622
MmeI TCCRAC 2 cut(s) 283, 533
MnlI CCTC 7 cut(s) 56, 112, 290, 306, 308, 349, 399
MseI TTAA 5 cut(s) 26, 72, 194, 203, 270
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 544
MspI CCGG 3 cut(s) 144, 177, 213
MspR9I CCNGG 2 cut(s) 145, 363
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 425
NciI CCSGG 1 cut(s) 145
NdeII GATC 1 cut(s) 469
NlaIII CATG 3 cut(s) 96, 129, 220
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 2 cut(s) 118, 165
NsbI TGCGCA 1 cut(s) 130
PaeR7I CTCGAG 1 cut(s) 297
PceI AGGCCT 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 197
PfeI GAWTC 4 cut(s) 5, 452, 497, 528
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 2 cut(s) 116, 477
PpuMI RGGWCCY 2 cut(s) 365, 440
PsiI TTATAA 1 cut(s) 239
Psp5II RGGWCCY 2 cut(s) 365, 440
Psp6I CCWGG 1 cut(s) 361
PspEI GGTNACC 1 cut(s) 165
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 3 cut(s) 365, 380, 440
PspPPI RGGWCCY 2 cut(s) 365, 440
PspXI VCTCGAGB 1 cut(s) 297
PstI CTGCAG 1 cut(s) 477
PstNI CAGNNNCTG 2 cut(s) 443, 455
PvuII CAGCTG 1 cut(s) 544
RsaI GTAC 3 cut(s) 88, 151, 520
RsaNI GTAC 3 cut(s) 87, 150, 519
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 5 cut(s) 26, 72, 194, 203, 270
SatI GCNGC 2 cut(s) 115, 476
Sau3AI GATC 1 cut(s) 469
Sau96I GGNCC 3 cut(s) 365, 380, 440
ScrFI CCNGG 2 cut(s) 145, 363
SduI GDGCHC 1 cut(s) 212
SetI ASST 8 cut(s) 104, 304, 358, 367, 421, 442, 508, 546
SfaNI GCATC 3 cut(s) 443, 491, 566
SfcI CTRYAG 1 cut(s) 473
Sfr274I CTCGAG 1 cut(s) 297
SinI GGWCC 2 cut(s) 365, 440
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 4 cut(s) 39, 133, 609, 622
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 1 cut(s) 162
SspMI CTAG 2 cut(s) 377, 631
StuI AGGCCT 1 cut(s) 338
StyD4I CCNGG 2 cut(s) 143, 361
StyI CCWWGG 1 cut(s) 523
TaaI ACNGT 2 cut(s) 224, 400
TaqI TCGA 3 cut(s) 22, 298, 560
TasI AATT 4 cut(s) 39, 133, 609, 622
TatI WGTACW 1 cut(s) 86
TfiI GAWTC 4 cut(s) 5, 452, 497, 528
Tru1I TTAA 5 cut(s) 26, 72, 194, 203, 270
Tru9I TTAA 5 cut(s) 26, 72, 194, 203, 270
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 2 cut(s) 118, 165
TseI GCWGC 2 cut(s) 114, 475
Tsp45I GTSAC 2 cut(s) 118, 165
TspDTI ATGAA 1 cut(s) 510
TspRI CASTG 1 cut(s) 330
Van91I CCANNNNNTGG 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 365, 440
XagI CCTNNNNNAGG 1 cut(s) 360
XhoI CTCGAG 1 cut(s) 297
XspI CTAG 2 cut(s) 377, 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.