MD11G1282200.v1.1

Non-specific lipid-transfer protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012814
Physical Location & Seq
Reverse (-)
40084123 .. 40085409
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1282200.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGACTCATTCAAGGACTTTCGATTGAGTCTAATCCCAATTACCCTCCTTATCATTTCACTAAGATGGGTTAATGGGCAGATTAGTACACCATGCACAACCTCAATGATAAGCAGCATCACTCCATGCGTAAATTTCATCACCGGGAGTACAAGCAGCGGCGCACCACCGACAGCCGGATGTTGCAATTCGTTAAAGTCAATAATGGGCACGGGCACGGACTGCGCTTGTCTTCTTATAACCGGCGGTGTCCCTTTCCAACTGCCCATTAACCAAACCCTTGCGCTTTCTCTTCCTCAAGTTTGTGGGATGGGAGCCGTCCCACTGCAATGCAAAGCCTCTGGTAGTCCTTTAGCTGCTCCAGGTTCTGGGTTACTAGGGCCAACACCCCCACCAACAGCTTCTTCACCTCTAAGTCCAGGAGATTCTATGGCGGTTGCAGCAGGTCCTGCACCAGAATCTGGAACATCTGATGATCTGCAGCCAGCATCTCCATCAGATGAACCAGAAGCTCCAACACAAAGTACCAAAGGAGTCGGAAGACCACAGCTGACCCCATCAGCATCTAGTTTAACCTATGTTCCCCCACCATCTTTCATACTGATAATTTTGGGAATTATGGTTTTCAAGTCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

213

Amino Acids

21.42

Weight (kDa)

4.81

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 27 - 111 1.7e-19 Probable lipid transfer
Tryp_alpha_amyl PF00234 36 - 111 5.4e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012814)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G48140 AT2G48140
fragaria_vesca FvH4_2g23210
malus_domestica MD03G1261400.v1.1 MD11G1281900.v1.1 MD11G1282200.v1.1
prunus_persica Prupe.8G237800_v2.0.a1
pyrus_communis pycom03g20940 pycom11g24990
rosa_chinensis RchiOBHm_Chr6g0290481
rosa_laevigata RLG00000012211
rosa_roxburghii Rroxscaffold_7G00177020
rosa_rugosa Rorug06G0212900 Rorug06G0212900
rosa_samantha Rh6AG323300 Rh6BG330500 Rh6DG323700
rosa_wichuraiana Rw6G028030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 239
Acc36I ACCTGC 1 cut(s) 434
AccB7I CCANNNNNTGG 2 cut(s) 368, 461
AciI CCGC 3 cut(s) 159, 246, 434
AcsI RAATTY 1 cut(s) 133
AfaI GTAC 3 cut(s) 88, 151, 526
AfiI CCNNNNNNNGG 3 cut(s) 176, 368, 461
AgsI TTSAA 2 cut(s) 13, 628
AjnI CCWGG 2 cut(s) 361, 418
AjuI GAANNNNNNNTTGG 2 cut(s) 388, 420
AluBI AGCT 4 cut(s) 356, 401, 512, 550
AluI AGCT 4 cut(s) 356, 401, 512, 550
AlwNI CAGNNNCTG 3 cut(s) 368, 449, 461
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 5 cut(s) 114, 156, 356, 440, 481
ApoI RAATTY 1 cut(s) 133
AspLEI GCGC 3 cut(s) 164, 227, 286
AspS9I GGNCC 2 cut(s) 380, 446
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 2 cut(s) 133, 399
AvaII GGWCC 1 cut(s) 446
BaeGI GKGCMC 2 cut(s) 212, 218
BbsI GAAGAC 2 cut(s) 224, 547
BbvI GCAGC 5 cut(s) 126, 168, 343, 452, 493
BccI CCATC 5 cut(s) 60, 304, 502, 565, 598
BceAI ACGGC 1 cut(s) 302
BciT130I CCWGG 2 cut(s) 363, 420
BcnI CCSGG 1 cut(s) 145
BfaI CTAG 2 cut(s) 377, 567
BfmI CTRYAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 6 cut(s) 115, 157, 160, 357, 441, 482
BlsI GCNGC 6 cut(s) 116, 158, 161, 358, 442, 483
Bme1390I CCNGG 3 cut(s) 145, 363, 420
Bme18I GGWCC 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 380, 446
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 3 cut(s) 145, 363, 420
BmsI GCATC 3 cut(s) 126, 497, 572
BpiI GAAGAC 2 cut(s) 224, 547
BpmI CTGGAG 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 368, 461
Bse118I RCCGGY 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 335
BseBI CCWGG 2 cut(s) 363, 420
BseGI GGATG 2 cut(s) 185, 315
BseLI CCNNNNNNNGG 3 cut(s) 176, 368, 461
BseMI GCAATG 1 cut(s) 335
BseSI GKGCMC 2 cut(s) 212, 218
BseXI GCAGC 5 cut(s) 126, 168, 343, 452, 493
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 1 cut(s) 382
BsiSI CCGG 3 cut(s) 144, 177, 243
BslFI GGGAC 2 cut(s) 236, 305
BslI CCNNNNNNNGG 3 cut(s) 176, 368, 461
BsmFI GGGAC 2 cut(s) 236, 305
BsnI GGCC 1 cut(s) 382
Bsp1286I GDGCHC 2 cut(s) 212, 218
Bsp143I GATC 1 cut(s) 475
BspACI CCGC 3 cut(s) 159, 246, 434
BspANI GGCC 1 cut(s) 382
BspLI GGNNCC 1 cut(s) 316
BspMAI CTGCAG 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 335
BsrFI RCCGGY 1 cut(s) 242
BssAI RCCGGY 1 cut(s) 242
BssMI GATC 1 cut(s) 475
Bst2UI CCWGG 2 cut(s) 363, 420
Bst6I CTCTTC 1 cut(s) 297
BstAPI GCANNNNNTGC 2 cut(s) 222, 449
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 2 cut(s) 62, 413
BstF5I GGATG 2 cut(s) 185, 315
BstHHI GCGC 3 cut(s) 164, 227, 286
BstKTI GATC 1 cut(s) 478
BstMBI GATC 1 cut(s) 475
BstMWI GCNNNNNNNGC 3 cut(s) 222, 440, 449
BstNI CCWGG 2 cut(s) 363, 420
BstSCI CCNGG 3 cut(s) 143, 361, 418
BstSFI CTRYAG 1 cut(s) 479
BstSLI GKGCMC 2 cut(s) 212, 218
BstV1I GCAGC 5 cut(s) 126, 168, 343, 452, 493
BstV2I GAAGAC 2 cut(s) 224, 547
BsuRI GGCC 1 cut(s) 382
BtsCI GGATG 2 cut(s) 185, 315
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 1 cut(s) 323
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 1 cut(s) 486
CaiI CAGNNNCTG 3 cut(s) 368, 449, 461
CfoI GCGC 3 cut(s) 164, 227, 286
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 380, 446
Csp6I GTAC 3 cut(s) 87, 150, 525
CviAII CATG 2 cut(s) 93, 126
CviJI RGCY 9 cut(s) 176, 317, 338, 356, 382, 401, 484, 512, 550
CviKI_1 RGCY 9 cut(s) 176, 317, 338, 356, 382, 401, 484, 512, 550
CviQI GTAC 3 cut(s) 87, 150, 525
DdeI CTNAG 2 cut(s) 62, 413
DpnI GATC 1 cut(s) 477
DpnII GATC 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 446
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 2 cut(s) 361, 418
FaeI CATG 2 cut(s) 96, 129
FaiI YATR 7 cut(s) 94, 127, 239, 431, 579, 599, 620
FaqI GGGAC 2 cut(s) 236, 305
FatI CATG 2 cut(s) 92, 125
Fnu4HI GCNGC 6 cut(s) 115, 157, 160, 357, 441, 482
FokI GGATG 2 cut(s) 192, 322
Fsp4HI GCNGC 6 cut(s) 115, 157, 160, 357, 441, 482
FspBI CTAG 2 cut(s) 377, 567
GlaI GCGC 3 cut(s) 163, 226, 285
GluI GCNGC 6 cut(s) 115, 157, 160, 357, 441, 482
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 1 cut(s) 382
HapII CCGG 3 cut(s) 144, 177, 243
HhaI GCGC 3 cut(s) 164, 227, 286
Hin1II CATG 2 cut(s) 96, 129
Hin6I GCGC 3 cut(s) 162, 225, 284
HinP1I GCGC 3 cut(s) 162, 225, 284
HinfI GANTC 5 cut(s) 5, 28, 425, 458, 534
HpaII CCGG 3 cut(s) 144, 177, 243
HphI GGTGA 2 cut(s) 133, 399
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 3 cut(s) 472, 499, 539
Hpy188III TCNNGA 1 cut(s) 462
Hpy8I GTNNAC 1 cut(s) 89
HpyCH4V TGCA 7 cut(s) 96, 186, 328, 333, 440, 452, 481
HpyF10VI GCNNNNNNNGC 3 cut(s) 222, 440, 449
HpyF3I CTNAG 2 cut(s) 62, 413
Hsp92II CATG 2 cut(s) 96, 129
HspAI GCGC 3 cut(s) 162, 225, 284
Kzo9I GATC 1 cut(s) 475
LmnI GCTCC 3 cut(s) 314, 364, 517
Lsp1109I GCAGC 5 cut(s) 126, 168, 343, 452, 493
LweI GCATC 3 cut(s) 126, 497, 572
MaeI CTAG 2 cut(s) 377, 567
MaeIII GTNAC 1 cut(s) 372
MalI GATC 1 cut(s) 477
MboI GATC 1 cut(s) 475
MboII GAAGA 4 cut(s) 224, 284, 396, 552
MhlI GDGCHC 2 cut(s) 212, 218
MluCI AATT 5 cut(s) 39, 133, 187, 606, 615
MlyI GAGTC 2 cut(s) 37, 543
MmeI TCCRAC 3 cut(s) 283, 517, 539
MnlI CCTC 5 cut(s) 56, 112, 306, 349, 420
MseI TTAA 4 cut(s) 72, 194, 270, 572
MslI CAYNNNNRTG 2 cut(s) 64, 328
MspA1I CMGCKG 2 cut(s) 159, 550
MspI CCGG 3 cut(s) 144, 177, 243
MspR9I CCNGG 3 cut(s) 145, 363, 420
MvaI CCWGG 2 cut(s) 363, 420
MwoI GCNNNNNNNGC 3 cut(s) 222, 440, 449
NciI CCSGG 1 cut(s) 145
NdeII GATC 1 cut(s) 475
NlaIII CATG 2 cut(s) 96, 129
NlaIV GGNNCC 1 cut(s) 316
PfeI GAWTC 2 cut(s) 425, 458
PflMI CCANNNNNTGG 2 cut(s) 368, 461
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 6 cut(s) 116, 158, 161, 358, 442, 483
PleI GAGTC 2 cut(s) 36, 542
PpsI GAGTC 2 cut(s) 36, 542
PpuMI RGGWCCY 1 cut(s) 446
PsiI TTATAA 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 2 cut(s) 361, 418
PspGI CCWGG 2 cut(s) 361, 418
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 2 cut(s) 380, 446
PspPPI RGGWCCY 1 cut(s) 446
PstI CTGCAG 1 cut(s) 483
PstNI CAGNNNCTG 3 cut(s) 368, 449, 461
PvuII CAGCTG 1 cut(s) 550
RsaI GTAC 3 cut(s) 88, 151, 526
RsaNI GTAC 3 cut(s) 87, 150, 525
RseI CAYNNNNRTG 2 cut(s) 64, 328
SaqAI TTAA 4 cut(s) 72, 194, 270, 572
SatI GCNGC 6 cut(s) 115, 157, 160, 357, 441, 482
Sau3AI GATC 1 cut(s) 475
Sau96I GGNCC 2 cut(s) 380, 446
SchI GAGTC 2 cut(s) 37, 543
ScrFI CCNGG 3 cut(s) 145, 363, 420
SduI GDGCHC 2 cut(s) 212, 218
SetI ASST 9 cut(s) 104, 358, 367, 403, 412, 448, 514, 552, 578
SfaNI GCATC 3 cut(s) 126, 497, 572
SfcI CTRYAG 1 cut(s) 479
SinI GGWCC 1 cut(s) 446
SmiMI CAYNNNNRTG 2 cut(s) 64, 328
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 5 cut(s) 39, 133, 187, 606, 615
SsiI CCGC 3 cut(s) 159, 246, 434
SspMI CTAG 2 cut(s) 377, 567
StyD4I CCNGG 3 cut(s) 143, 361, 418
TaqI TCGA 1 cut(s) 22
TasI AATT 5 cut(s) 39, 133, 187, 606, 615
TatI WGTACW 2 cut(s) 86, 149
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 2 cut(s) 425, 458
Tru1I TTAA 4 cut(s) 72, 194, 270, 572
Tru9I TTAA 4 cut(s) 72, 194, 270, 572
TscAI CASTG 1 cut(s) 330
TseI GCWGC 5 cut(s) 114, 156, 356, 440, 481
TspDTI ATGAA 3 cut(s) 127, 516, 586
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 1 cut(s) 330
Van91I CCANNNNNTGG 2 cut(s) 368, 461
VpaK11BI GGWCC 1 cut(s) 446
XapI RAATTY 1 cut(s) 133
XspI CTAG 2 cut(s) 377, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.