Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0005776

Physical Location & Seq

Genomic Coordinates Forward (+)

20692687 .. 20699717

Gene Structure 7031 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g25570.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4521 bp

ATGGCGTCCGCGTCGTGGATCACGTCCACCTCGTGTTCCTCCTCCGTGATTCACTCCCAAGACGACACCTCATCATCAGCTCCGGCCATCTTTCAATGGCTGAGATTCGTCTTCCTCTCCCCCTGCCCTCAGCGAGCTCTGTTATCCTCCATCAATCTCTTGTTCCTCCTCACACTCCTCGCTTTCGCAATCCAAAAGCTCTACTCGAGGCTCACCTCCAGCCGCGGCGGCGCTTCGGAGCTCGACAAGCCGTTGATAACAAACAGCCGAGCTCACCGTCCCAGCACCACGCTCTGCTTCAAGCTCTCGCTTACCGTGAGCCTTTTCTTGACTCTATGTTACTCCATTGTGTGCATTTTAGCTTTCACGAGACGAAGTAGTAGTACCGAGTCTCTATGGAAGACCGTGGACGGGTTGTTCTGGCTGGTGCAAGCTGTAACTCATGGGGTTGTTACAGTGCTGGTGGCGCATGAGAAGAGATTCGAAGCTGTGAAGCACCCGCTTTCGCTTCGGATTTACTGGCTGGCTAATTTCGTTGCGGTTTCTCTGTTTACAGCTTCTGGTGTGATTCGTTTGGTTCATAATGAAGGCAGTATGAGATTGGATGATGTGGTTTCCTTTGTGTCTTTGCCGTTATCTGTTGTTCTTGCTGTTGTTGCGGTTAGAGGATCCACTGGGATTCGAGTGATGATTAACGGAGAAGAGTCTAACGGAGTGTATGAGCCGTTACTGAGCAAGTCCAATGTGACTGGTTTCGCTTCAGCTTCGTTCATTAGTAAGACGTTTTGGATTTGGATGAATCCTCTGCTCCGTAAAGGGTACAAGTCCCCTTTGAAAGTTGATGAAGTTCCGACGCTTGCGCCGGAGCACAGAGCCGAGAGAATGTCGCTCATTTTCGAATCCAACTGGCCTAAACCGGAGGAGAAGTCGGAGCATCCGGTTCGAACCACATTGCTGCGGTGCTTCTGGAAAGAGATTGCTTTCACTGCTTTTCTTGCGGTGATCAGGCTGTGTGTCATGTATGTAGGTCCTGTGCTGATCCAGAGCTTTGTGGATTTCACTGCTGGGAAAAGAAGCTCGCCGTTTGAGGGCTACTATTTGGTGTTGATTCTTCTGTGTGCGAAGTTCGTCGAGGTGTTGTGCACTCACCAGTTTAACTTCAACTCACAGAAGCTGGGGATGCTCATAAGATCAACTCTCATTACTTCTTTGTACAAGAAGGGCCTGAGGCTGACCTGCTCGGCGCGGCAGGCTCATGGTGTTGGACAGATTGTGAATTACATGGCTGTCGATGCGCAGCAGCTATCGGACATGATGCTGCAGCTTCATGCCATATGGATGATGCCAGTGCAGCTCATCATTGCTTTAGTCCTGCTGTACAACAACCTCGGAGCAGCGGTGATCACAGCAATGATAGGGATCATTGGTGTGCTTGTGTTTGTGGTGTTTGGGACGAGGAGGAACAATAGGTTTCAGTTCAATCTGATGAAGCAAAGGGACTCGAGGATGAAGGCCACGAATGAGATGCTTAATTACATGAGGGTCATCAAGTTCCAGGCGTGGGAGGAACACTTCAACAAGAGGATTCAGACTTTTCGTGAATCTGAGTTTAGCTGGCTGACCAAGTTTATGTACTCCATCTCGGCTAATGTTGTGCTCATGTGGTGCACACCCTTACTTATATCAACTGTCACATTCGCCACTGCCTTGTTTTTGGGTGTCAAACTTGATGCTGGGACGGTGTTCACTACCACAACCATTTTCAAAATTCTGCAGGAGCCTATTAGGACTTTCCCACAATCTATGATTTCGATTTCTCAAGCTATGATCTCGCTTGGGAGGTTGGACCGGTATATGAGTAGTAGGGAGCTGGTGGAGGGTTCGGTTGAGAGAGAAGAAGGTTGTGACAGTAGAGTTGCTGTGGAGGTTAAGGATGGTGCATTTAGCTGGGATGATGAAAGCAATGAAGCGGTATTGAAGAATATAAACTTGACTGTCAACAAAGGCGAGCTAACTGCGATTGTTGGGACAGTAGGATCTGGAAAGTCTTCCCTGCTGGCCTCAATCCTTGGTGAGATGCACAAATTGTCTGGAAAGGTAAAAGTTTGCGGGACAACTGCTTATGTGGCTCAAACATCATGGATTCAGAATGGGACAATTGAAGAAAACATCCTATTTGGTTCGCCAATGGACAGAGCGAGATACCAGGAAGTTATGAGGGTCTGTTGCTTGGAGAAAGACATGGAAATGATGGAGTATGGAGATCAAACCGAGATAGGAGAGCGTGGCATCAACCTAAGTGGTGGACAGAAACAAAGGATACAGCTTGCCAGGGCAGTATACCAGGACTGTGATATATATCTTCTTGATGATGTCTTCAGTGCTGTTGATGCTCATACCGGATCAGAAATATTCAAGGAATGTGTGCGGGGAGCACTTAAGAACAAGACCATTTTACTAGTAACCCATCAAGTTGACTTCTTGCATAATGTTGATCTTATAGTGGTCATGAGAGAGGGAATGATAGTACAAGCAGGAAAGTACAACGATTTGCTAAGCCTCGATTTCAAAGCACTTGTAGTTGCGCATGAAAGCTCCATGGAGCTTGTAGAAATGGGCACAGCTATGCCTGGTGAAAGTACTTCTCCCAAACCACAGATATCTCGGCAGTCCTCTTCAAAACATGGAGAAGCGAATGGTGAAAACAACTCTCAGCTGGATGAACCCAAGTCCAAAGATGGAACATCTAAGCTCATCAAAGAAGAGGAGAAAGAATCAGGCAAAGTCAGCTTGCAGAATTACAAAATTTATTGCACAGAGGCTTTTGGATGGTGGGGTGTGGTTCTAGTGTTGTCTCTCTCTCTTGTGTGGCAAGGATCACTTATGGCTGGTGATTATTGGCTGGCCTATGAAACTTCTGCAAAGCGAGCAGCATCATTTGACCCTTCTGTGTTCATTACTGTCTATGCTATTATCGCAGTTGTTTCATTCTTCCTGGTATTGGTGAGAGCATTTACTGTGACAATTGTGGGACTTACAACAGCTCAAATTTTCTTCAAGCAAATTCTTCACAGCATCCTTCATGCCCCCATGTCATTCTTTGATACTACACCTTCTGGAAGAATTCTGAGTAGGGCATCAACTGATCAGACGAATATCGACTTATTCCTTCCCTTCATGTTGGGCGTTACCATTGCCATGTATATCACTGTGCTCAGTATCTTCATCGTCGTATGTCAAAACTCGTGGCCAACAATTTTCTTACTGATACCACTTCTTTGGCTTAATATATGGTACCGGGGTTACTATCTTGCATCATCTCGTGAATTAACCCGACTTGATTCAATCACGAAGGCGCCTGTTATCCATCATTTCTCAGAAAGCATATCTGGGGTTATGACAATCCGATCCTTCAGAAACCAGAACAAGTTTACCAAGGAAAATGTTAGAAGAGTGAATGCCAATTTGCGTATGGATTTCCACAATAATGGCTCCAATGAGTGGTTGGGATTTCGTCTAGAACTTCTTGGGAGCTTAATTCTCTGCATTTCCACTTTGTTCATGATCTTGCTGCCAAGCAGTATAGTAAAGCCTGAGAATATTGGCTTATCACTCTCCTATGGATTGTCCCTCAATGGTGTACTATTTTGGGCCATATATATGAGCTGCTTCGTTGAAAATAGGATGGTGTCAGTTGAGAGGATAAAGCAGTTCTGTAACATTCCATCAGAAGCAGCATGGAAGATAGTAGATCGTGTCCCTCCTATGAATTGGCCAACTCATGGAAACGTTGAGCTGAAAGACTTGCAGGTCAGATATCGTCCGAATACTCCCTTGGTTCTGAAAGGCATTAGTCTGAGCATTAATGGGGGTGAGAAGGTTGGTGTTGTTGGTCGAACTGGGAGTGGGAAATCCACTTTAATCCAGGTGTTCTTTAGGCTGGTGGAACCTTCAGCGGGAAAAATTATCATTGATGGCATTGACATATGCACAATAGGGCTTCATGATCTCCGGTCTAGCTTTGGGATCATTCCTCAAGAACCTGTCCTTTTCGAAGGCACTGTGAGAAGCAACATTGATCCAATCGGGGTGTATTCAGATGAAGAGATTTGGAAGAGCTTGGAGCGGTGCCAACTTAAAGATGTTGTTGCTGCAAAAACTGAAAAACTCAATGCTTTAGTGGCTGATGATGGAGATAACTGGAGCGTGGGGCAAAGACAGCTTCTCTGTTTGGGACGAGTTATGTTAAAGCGCAGCAGGCTGTTGTTCATGGATGAGGCCACGGCTTCAGTTGATTCACAGACGGATGCTGCGATACAAAAGATCATCAGAGAGGACTTTGCTGCATGTACAATCATCAGCATTGCTCACAGAATACCAACAGTGATGGACTGCAATAGAGTGTTAGTAGTAGATGCAGGGAGGGCTAAAGAGTTTGACTCTCCATCACACCTGCTCGAGAGGCGGTCGCTGTTTGGGGCATTGGTTCAGGAGTATGCCAACCGCTCAGAAGGAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006855 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009624 GO:0009628 GO:0009987 GO:0010035 GO:0010118 GO:0012505 GO:0015399 GO:0015405 GO:0015893 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0030054 GO:0031090 GO:0042221 GO:0042493 GO:0042623 GO:0042626 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04071 ko04976 ko04977 ko05206 map01523 map01524 map02010 map04071 map04976 map04977 map05206

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1507

Amino Acids

168.39

Weight (kDa)

8.57

Isoelectric Point (pI)

37.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	328 - 594	3.1e-26	ABC transporter transmembrane region
ABC_tran	PF00005	659 - 792	2.5e-21	ABC transporter
ABC_membrane	PF00664	963 - 1200	4.2e-31	ABC transporter transmembrane region
ABC_tran	PF00005	1281 - 1429	2.9e-26	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47800 AT3G62700
fragaria_vesca	FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570
malus_domestica	MD03G1286300.v1.1 MD11G1313300.v1.1 MD11G1313800.v1.1 MD11G1314300.v1.1
prunus_persica	Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1
pyrus_communis	pycom03g22680 pycom03g22690 pycom11g27270
rosa_chinensis	RchiOBHm_Chr6g0293781
rosa_laevigata	RLG00000011924
rosa_multiflora	Rmu_sc0005641.1_g000002
rosa_roxburghii	Rroxscaffold_7G00173910
rosa_rugosa	Rorug06G0238000
rosa_samantha	Rh6AG351300 Rh6BG358200 Rh6DG352000
rosa_wichuraiana	Rw6G030560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	4461
AasI	GACNNNNNNGTC	1 cut(s)	3809
Acc16I	TGCGCA	2 cut(s)	1296, 2586
Acc36I	ACCTGC	3 cut(s)	1244, 3799, 4461
Acc65I	GGTACC	1 cut(s)	3294
AccB1I	GGYRCC	3 cut(s)	3294, 3355, 4128
AccB7I	CCANNNNNTGG	2 cut(s)	3501, 3782
AccBSI	CCGCTC	2 cut(s)	4126, 4506
AccI	GTMKAC	1 cut(s)	2340
AccII	CGCG	3 cut(s)	11, 225, 1246
AclI	AACGTT	1 cut(s)	3789
AcoI	YGGCCR	3 cut(s)	84, 3248, 3773
AcsI	RAATTY	6 cut(s)	1767, 2805, 3048, 3063, 3123, 4515
AcuI	CTGAAG	5 cut(s)	744, 2362, 3397, 3936, 4272
AcyI	GRCGYC	2 cut(s)	5, 3356
AdeI	CACNNNGTG	1 cut(s)	33
AfiI	CCNNNNNNNGG	8 cut(s)	15, 411, 1088, 1561, 3001, 3501, 3782, 3956
AflII	CTTAAG	1 cut(s)	2438
AgeI	ACCGGT	1 cut(s)	1848
AhlI	ACTAGT	1 cut(s)	2458
AjiI	CACGTC	1 cut(s)	24
AjnI	CCWGG	7 cut(s)	1554, 2205, 2330, 2343, 2629, 2994, 3924
AjuI	GAANNNNNNNTTGG	2 cut(s)	3818, 3850
AloI	GAACNNNNNNTCC	2 cut(s)	401, 433
Alw21I	GWGCWC	9 cut(s)	139, 243, 274, 870, 1145, 1659, 1670, 2437, 3216
Alw26I	GTCTC	3 cut(s)	364, 396, 2859
Alw44I	GTGCAC	2 cut(s)	1141, 1666
AlwNI	CAGNNNCTG	2 cut(s)	560, 1174
Ama87I	CYCGRG	3 cut(s)	205, 1501, 4457
ApaLI	GTGCAC	2 cut(s)	1141, 1666
ApoI	RAATTY	6 cut(s)	1767, 2805, 3048, 3063, 3123, 4515
AseI	ATTAAT	1 cut(s)	3864
AsiGI	ACCGGT	1 cut(s)	1848
Asp700I	GAANNNNTTC	2 cut(s)	479, 2047
Asp718I	GGTACC	1 cut(s)	3294
AspLEI	GCGC	8 cut(s)	233, 469, 862, 1246, 1297, 2587, 3358, 4254
AspS9I	GGNCC	4 cut(s)	1028, 1222, 1846, 3651
AsuC2I	CCSGG	1 cut(s)	3299
AsuII	TTCGAA	4 cut(s)	483, 897, 943, 4053
AvaI	CYCGRG	3 cut(s)	205, 1501, 4457
AvaII	GGWCC	2 cut(s)	1028, 1846
AxyI	CCTNAGG	1 cut(s)	1226
BaeGI	GKGCMC	3 cut(s)	1145, 1670, 2621
BaeI	ACNNNNGTAYC	2 cut(s)	3286, 3319
BalI	TGGCCA	2 cut(s)	3250, 3775
BamHI	GGATCC	1 cut(s)	668
BanI	GGYRCC	3 cut(s)	3294, 3355, 4128
BanII	GRGCYC	3 cut(s)	139, 243, 274
BarI	GAAGNNNNNNTAC	2 cut(s)	367, 399
BauI	CACGAG	4 cut(s)	31, 367, 3244, 3321
BbsI	GAAGAC	4 cut(s)	103, 407, 2040, 2368
Bbv12I	GWGCWC	9 cut(s)	139, 243, 274, 870, 1145, 1659, 1670, 2437, 3216
BbvCI	CCTCAGC	1 cut(s)	129
BceAI	ACGGC	5 cut(s)	235, 616, 709, 1066, 4299
BcgI	CGANNNNNNTGC	6 cut(s)	923, 957, 1507, 1541, 1997, 2031
BciT130I	CCWGG	7 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3926
BciVI	GTATCC	1 cut(s)	2313
BclI	TGATCA	3 cut(s)	1002, 1401, 3145
BcnI	CCSGG	1 cut(s)	3299
BcoDI	GTCTC	3 cut(s)	364, 396, 2859
BcuI	ACTAGT	1 cut(s)	2458
BfaI	CTAG	4 cut(s)	2459, 2846, 3518, 4017
BfmI	CTRYAG	2 cut(s)	1319, 1772
BfoI	RGCGCY	2 cut(s)	234, 3359
BfrI	CTTAAG	1 cut(s)	2438
BfuAI	ACCTGC	3 cut(s)	1244, 3799, 4461
BfuI	GTATCC	1 cut(s)	2313
BglI	GCCNNNNNGGC	1 cut(s)	228
BlpI	GCTNAGC	1 cut(s)	2555
BmcAI	AGTACT	1 cut(s)	2641
Bme1390I	CCNGG	8 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3299, 3926
Bme18I	GGWCC	2 cut(s)	1028, 1846
BmeT110I	CYCGRG	3 cut(s)	205, 1501, 4457
BmgBI	CACGTC	1 cut(s)	24
BmgT120I	GGNCC	4 cut(s)	1028, 1222, 1846, 3651
BmiI	GGNNCC	7 cut(s)	670, 1779, 3296, 3357, 3493, 3948, 4130
BmrFI	CCNGG	8 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3299, 3926
BmrI	ACTGGG	2 cut(s)	684, 3909
BmuI	ACTGGG	2 cut(s)	684, 3909
BpiI	GAAGAC	4 cut(s)	103, 407, 2040, 2368
BplI	GAGNNNNNCTC	2 cut(s)	4424, 4456
BpmI	CTGGAG	2 cut(s)	202, 4222
Bpu10I	CCTNAGC	1 cut(s)	129
Bpu1102I	GCTNAGC	1 cut(s)	2555
Bpu14I	TTCGAA	4 cut(s)	483, 897, 943, 4053
BpuEI	CTTGAG	2 cut(s)	1803, 4020
BpuMI	CCSGG	1 cut(s)	3299
BsaBI	GATNNNNATC	2 cut(s)	1418, 2358
BsaHI	GRCGYC	2 cut(s)	5, 3356
BsaWI	WCCGGW	5 cut(s)	916, 937, 1848, 2399, 4011
BsaXI	ACNNNNNCTCC	4 cut(s)	911, 941, 1383, 1413
Bsc4I	CCNNNNNNNGG	8 cut(s)	15, 411, 1088, 1561, 3001, 3501, 3782, 3956
Bse118I	RCCGGY	1 cut(s)	1848
Bse1I	ACTGG	8 cut(s)	524, 679, 754, 911, 1150, 1346, 3904, 4205
Bse21I	CCTNAGG	1 cut(s)	1226
Bse3DI	GCAATG	6 cut(s)	950, 1359, 1416, 1969, 3192, 4362
Bse8I	GATNNNNATC	2 cut(s)	1418, 2358
BseBI	CCWGG	7 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3926
BseJI	GATNNNNATC	2 cut(s)	1418, 2358
BseLI	CCNNNNNNNGG	8 cut(s)	15, 411, 1088, 1561, 3001, 3501, 3782, 3956
BseMI	GCAATG	6 cut(s)	950, 1359, 1416, 1969, 3192, 4362
BseNI	ACTGG	8 cut(s)	524, 679, 754, 911, 1150, 1346, 3904, 4205
BseRI	GAGGAG	6 cut(s)	31, 158, 167, 935, 1471, 2780
BseSI	GKGCMC	3 cut(s)	1145, 1670, 2621
BseYI	CCCAGC	5 cut(s)	281, 1064, 1174, 1733, 1947
BsgI	GTGCAG	1 cut(s)	1370
Bsh1236I	CGCG	3 cut(s)	11, 225, 1246
Bsh1285I	CGRYCG	1 cut(s)	4469
BshNI	GGYRCC	3 cut(s)	3294, 3355, 4128
BshTI	ACCGGT	1 cut(s)	1848
BsiEI	CGRYCG	1 cut(s)	4469
BsiHKAI	GWGCWC	9 cut(s)	139, 243, 274, 870, 1145, 1659, 1670, 2437, 3216
BsiHKCI	CYCGRG	3 cut(s)	205, 1501, 4457
BsiSI	CCGG	8 cut(s)	83, 863, 917, 938, 1849, 2400, 3298, 4012
BslI	CCNNNNNNNGG	8 cut(s)	15, 411, 1088, 1561, 3001, 3501, 3782, 3956
BsmAI	GTCTC	3 cut(s)	364, 396, 2859
BsmBI	CGTCTC	1 cut(s)	364
BsmI	GAATGC	1 cut(s)	3463
BsoBI	CYCGRG	3 cut(s)	205, 1501, 4457
Bsp119I	TTCGAA	4 cut(s)	483, 897, 943, 4053
Bsp1407I	TGTACA	3 cut(s)	1212, 1377, 4349
Bsp1720I	GCTNAGC	1 cut(s)	2555
Bsp19I	CCATGG	1 cut(s)	2598
BspFNI	CGCG	3 cut(s)	11, 225, 1246
BspHI	TCATGA	3 cut(s)	2508, 3561, 4003
BspLI	GGNNCC	7 cut(s)	670, 1779, 3296, 3357, 3493, 3948, 4130
BspMAI	CTGCAG	2 cut(s)	1323, 1776
BspMI	ACCTGC	3 cut(s)	1244, 3799, 4461
BspQI	GCTCTTC	1 cut(s)	4109
BspT104I	TTCGAA	4 cut(s)	483, 897, 943, 4053
BspT107I	GGYRCC	3 cut(s)	3294, 3355, 4128
BspTI	CTTAAG	1 cut(s)	2438
BsrBI	CCGCTC	2 cut(s)	4126, 4506
BsrDI	GCAATG	6 cut(s)	950, 1359, 1416, 1969, 3192, 4362
BsrFI	RCCGGY	1 cut(s)	1848
BsrGI	TGTACA	3 cut(s)	1212, 1377, 4349
BsrI	ACTGG	8 cut(s)	524, 679, 754, 911, 1150, 1346, 3904, 4205
BssAI	RCCGGY	1 cut(s)	1848
BssNAI	GTATAC	1 cut(s)	2341
BssNI	GRCGYC	2 cut(s)	5, 3356
BssSI	CACGAG	4 cut(s)	31, 367, 3244, 3321
BssT1I	CCWWGG	4 cut(s)	2068, 2598, 3435, 3834
Bst1107I	GTATAC	1 cut(s)	2341
Bst2BI	CACGAG	4 cut(s)	31, 367, 3244, 3321
Bst2UI	CCWGG	7 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3926
Bst6I	CTCTTC	7 cut(s)	470, 696, 2680, 2757, 3445, 4098, 4109
BstACI	GRCGYC	2 cut(s)	5, 3356
BstAFI	CTTAAG	1 cut(s)	2438
BstAUI	TGTACA	3 cut(s)	1212, 1377, 4349
BstBI	TTCGAA	4 cut(s)	483, 897, 943, 4053
BstDSI	CCRYGG	4 cut(s)	223, 405, 2598, 4281
BstFNI	CGCG	3 cut(s)	11, 225, 1246
BstH2I	RGCGCY	2 cut(s)	234, 3359
BstHHI	GCGC	8 cut(s)	233, 469, 862, 1246, 1297, 2587, 3358, 4254
BstMAI	GTCTC	3 cut(s)	364, 396, 2859
BstMCI	CGRYCG	1 cut(s)	4469
BstNI	CCWGG	7 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3926
BstNSI	RCATGY	1 cut(s)	4350
BstSCI	CCNGG	8 cut(s)	1554, 2205, 2330, 2343, 2629, 2994, 3297, 3924
BstSFI	CTRYAG	2 cut(s)	1319, 1772
BstSLI	GKGCMC	3 cut(s)	1145, 1670, 2621
BstUI	CGCG	3 cut(s)	11, 225, 1246
BstV2I	GAAGAC	4 cut(s)	103, 407, 2040, 2368
BstX2I	RGATCY	2 cut(s)	668, 2036
BstXI	CCANNNNNNTGG	2 cut(s)	3279, 3488
BstYI	RGATCY	2 cut(s)	668, 2036
BstZ17I	GTATAC	1 cut(s)	2341
Bsu36I	CCTNAGG	1 cut(s)	1226
BsuI	GTATCC	1 cut(s)	2313
BtgI	CCRYGG	4 cut(s)	223, 405, 2598, 4281
BtrI	CACGTC	1 cut(s)	24
BtsI	GCAGTG	3 cut(s)	984, 1059, 1701
BveI	ACCTGC	3 cut(s)	1244, 3799, 4461
CaiI	CAGNNNCTG	2 cut(s)	560, 1174
CciI	TCATGA	3 cut(s)	2508, 3561, 4003
CfoI	GCGC	8 cut(s)	233, 469, 862, 1246, 1297, 2587, 3358, 4254
Cfr10I	RCCGGY	1 cut(s)	1848
Cfr13I	GGNCC	4 cut(s)	1028, 1222, 1846, 3651
Cfr42I	CCGCGG	1 cut(s)	226
CseI	GACGC	1 cut(s)	862
CspAI	ACCGGT	1 cut(s)	1848
CspCI	CAANNNNNGTGG	4 cut(s)	2281, 2316, 3227, 3262
DinI	GGCGCC	1 cut(s)	3357
DraIII	CACNNNGTG	1 cut(s)	33
DrdI	GACNNNNNNGTC	1 cut(s)	3809
DseDI	GACNNNNNNGTC	1 cut(s)	3809
EaeI	YGGCCR	3 cut(s)	84, 3248, 3773
Eam1104I	CTCTTC	7 cut(s)	470, 696, 2680, 2757, 3445, 4098, 4109
EarI	CTCTTC	7 cut(s)	470, 696, 2680, 2757, 3445, 4098, 4109
Ecl136II	GAGCTC	3 cut(s)	137, 241, 272
Eco130I	CCWWGG	4 cut(s)	2068, 2598, 3435, 3834
Eco24I	GRGCYC	3 cut(s)	139, 243, 274
Eco32I	GATATC	2 cut(s)	2661, 3818
Eco47I	GGWCC	2 cut(s)	1028, 1846
Eco53kI	GAGCTC	3 cut(s)	137, 241, 272
Eco57I	CTGAAG	5 cut(s)	744, 2362, 3397, 3936, 4272
Eco81I	CCTNAGG	1 cut(s)	1226
Eco88I	CYCGRG	3 cut(s)	205, 1501, 4457
EcoICRI	GAGCTC	3 cut(s)	137, 241, 272
EcoO109I	RGGNCCY	2 cut(s)	1028, 1222
EcoRI	GAATTC	1 cut(s)	3123
EcoRII	CCWGG	7 cut(s)	1554, 2205, 2330, 2343, 2629, 2994, 3924
EcoRV	GATATC	2 cut(s)	2661, 3818
EcoT14I	CCWWGG	4 cut(s)	2068, 2598, 3435, 3834
EcoT38I	GRGCYC	3 cut(s)	139, 243, 274
EgeI	GGCGCC	1 cut(s)	3357
EheI	GGCGCC	1 cut(s)	3357
ErhI	CCWWGG	4 cut(s)	2068, 2598, 3435, 3834
Esp3I	CGTCTC	1 cut(s)	364
FalI	AAGNNNNNCTT	2 cut(s)	2865, 2897
FauI	CCCGC	4 cut(s)	507, 2102, 2421, 3949
FauNDI	CATATG	2 cut(s)	1334, 3986
FbaI	TGATCA	3 cut(s)	1002, 1401, 3145
FblI	GTMKAC	1 cut(s)	2340
FriOI	GRGCYC	3 cut(s)	139, 243, 274
FspBI	CTAG	4 cut(s)	2459, 2846, 3518, 4017
FspI	TGCGCA	2 cut(s)	1296, 2586
GlaI	GCGC	8 cut(s)	232, 468, 861, 1245, 1296, 2586, 3357, 4253
GsaI	CCCAGC	5 cut(s)	285, 1068, 1178, 1737, 1951
GsuI	CTGGAG	2 cut(s)	202, 4222
HaeII	RGCGCY	2 cut(s)	234, 3359
HapII	CCGG	8 cut(s)	83, 863, 917, 938, 1849, 2400, 3298, 4012
HgaI	GACGC	1 cut(s)	862
HhaI	GCGC	8 cut(s)	233, 469, 862, 1246, 1297, 2587, 3358, 4254
Hin1I	GRCGYC	2 cut(s)	5, 3356
Hin6I	GCGC	8 cut(s)	231, 467, 860, 1244, 1295, 2585, 3356, 4252
HinP1I	GCGC	8 cut(s)	231, 467, 860, 1244, 1295, 2585, 3356, 4252
HincII	GTYRAC	2 cut(s)	1999, 2476
HindII	GTYRAC	2 cut(s)	1999, 2476
HpaII	CCGG	8 cut(s)	83, 863, 917, 938, 1849, 2400, 3298, 4012
Hpy99I	CGWCG	4 cut(s)	16, 856, 1133, 3233
HpyCH4IV	ACGT	3 cut(s)	23, 782, 3789
HpySE526I	ACGT	3 cut(s)	23, 782, 3789
Hsp92I	GRCGYC	2 cut(s)	5, 3356
HspAI	GCGC	8 cut(s)	231, 467, 860, 1244, 1295, 2585, 3356, 4252
KasI	GGCGCC	1 cut(s)	3355
KpnI	GGTACC	1 cut(s)	3298
Ksp22I	TGATCA	3 cut(s)	1002, 1401, 3145
KspI	CCGCGG	1 cut(s)	226
LguI	GCTCTTC	1 cut(s)	4109
MaeI	CTAG	4 cut(s)	2459, 2846, 3518, 4017
MaeII	ACGT	3 cut(s)	23, 782, 3789
MbiI	CCGCTC	2 cut(s)	4126, 4506
MfeI	CAATTG	2 cut(s)	2157, 3024
MflI	RGATCY	2 cut(s)	668, 2036
MlsI	TGGCCA	2 cut(s)	3250, 3775
MluNI	TGGCCA	2 cut(s)	3250, 3775
Mly113I	GGCGCC	1 cut(s)	3356
MlyI	GAGTC	5 cut(s)	325, 398, 713, 1493, 4433
MmeI	TCCRAC	5 cut(s)	875, 909, 927, 1243, 1824
Mox20I	TGGCCA	2 cut(s)	3250, 3775
MroXI	GAANNNNTTC	2 cut(s)	479, 2047
MscI	TGGCCA	2 cut(s)	3250, 3775
MslI	CAYNNNNRTG	8 cut(s)	1337, 1409, 1427, 2246, 2624, 3197, 3486, 3659
Msp20I	TGGCCA	2 cut(s)	3250, 3775
MspA1I	CMGCKG	4 cut(s)	225, 1397, 2716, 3956
MspCI	CTTAAG	1 cut(s)	2438
MspI	CCGG	8 cut(s)	83, 863, 917, 938, 1849, 2400, 3298, 4012
MspR9I	CCNGG	8 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3299, 3926
MunI	CAATTG	2 cut(s)	2157, 3024
Mva1269I	GAATGC	1 cut(s)	3463
MvaI	CCWGG	7 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3926
MvnI	CGCG	3 cut(s)	11, 225, 1246
NarI	GGCGCC	1 cut(s)	3356
NciI	CCSGG	1 cut(s)	3299
NcoI	CCATGG	1 cut(s)	2598
NdeI	CATATG	2 cut(s)	1334, 3986
NlaIV	GGNNCC	7 cut(s)	670, 1779, 3296, 3357, 3493, 3948, 4130
NmeAIII	GCCGAG	5 cut(s)	293, 901, 1220, 1622, 2644
NmuCI	GTSAC	4 cut(s)	745, 1690, 1904, 3019
NsbI	TGCGCA	2 cut(s)	1296, 2586
NspI	RCATGY	1 cut(s)	4350
NspV	TTCGAA	4 cut(s)	483, 897, 943, 4053
PaeR7I	CTCGAG	3 cut(s)	205, 1501, 4457
PagI	TCATGA	3 cut(s)	2508, 3561, 4003
PaqCI	CACCTGC	1 cut(s)	4461
PciSI	GCTCTTC	1 cut(s)	4109
PcsI	WCGNNNNNNNCGW	3 cut(s)	20, 29, 4310
PctI	GAATGC	1 cut(s)	3463
PdmI	GAANNNNTTC	2 cut(s)	479, 2047
PflMI	CCANNNNNTGG	2 cut(s)	3501, 3782
PinAI	ACCGGT	1 cut(s)	1848
PleI	GAGTC	5 cut(s)	325, 397, 712, 1493, 4433
PluTI	GGCGCC	1 cut(s)	3359
PpsI	GAGTC	5 cut(s)	325, 397, 712, 1493, 4433
PpuMI	RGGWCCY	1 cut(s)	1028
PshBI	ATTAAT	1 cut(s)	3864
Psp124BI	GAGCTC	3 cut(s)	139, 243, 274
Psp1406I	AACGTT	1 cut(s)	3789
Psp5II	RGGWCCY	1 cut(s)	1028
Psp6I	CCWGG	7 cut(s)	1554, 2205, 2330, 2343, 2629, 2994, 3924
PspFI	CCCAGC	5 cut(s)	281, 1064, 1174, 1733, 1947
PspGI	CCWGG	7 cut(s)	1554, 2205, 2330, 2343, 2629, 2994, 3924
PspN4I	GGNNCC	7 cut(s)	670, 1779, 3296, 3357, 3493, 3948, 4130
PspPI	GGNCC	4 cut(s)	1028, 1222, 1846, 3651
PspPPI	RGGWCCY	1 cut(s)	1028
PspXI	VCTCGAGB	2 cut(s)	205, 1501
PstI	CTGCAG	2 cut(s)	1323, 1776
PstNI	CAGNNNCTG	2 cut(s)	560, 1174
PsuI	RGATCY	2 cut(s)	668, 2036
PvuII	CAGCTG	1 cut(s)	2716
RseI	CAYNNNNRTG	8 cut(s)	1337, 1409, 1427, 2246, 2624, 3197, 3486, 3659
SacI	GAGCTC	3 cut(s)	139, 243, 274
SacII	CCGCGG	1 cut(s)	226
SapI	GCTCTTC	1 cut(s)	4109
Sau96I	GGNCC	4 cut(s)	1028, 1222, 1846, 3651
ScaI	AGTACT	1 cut(s)	2641
SchI	GAGTC	5 cut(s)	325, 398, 713, 1493, 4433
ScrFI	CCNGG	8 cut(s)	1556, 2207, 2332, 2345, 2631, 2996, 3299, 3926
SfcI	CTRYAG	2 cut(s)	1319, 1772
SfoI	GGCGCC	1 cut(s)	3357
Sfr274I	CTCGAG	3 cut(s)	205, 1501, 4457
Sfr303I	CCGCGG	1 cut(s)	226
SfuI	TTCGAA	4 cut(s)	483, 897, 943, 4053
SgrBI	CCGCGG	1 cut(s)	226
SinI	GGWCC	2 cut(s)	1028, 1846
SlaI	CTCGAG	3 cut(s)	205, 1501, 4457
SmiMI	CAYNNNNRTG	8 cut(s)	1337, 1409, 1427, 2246, 2624, 3197, 3486, 3659
SmlI	CTYRAG	6 cut(s)	205, 1501, 1818, 2438, 4035, 4457
SmoI	CTYRAG	6 cut(s)	205, 1501, 1818, 2438, 4035, 4457
SpeI	ACTAGT	1 cut(s)	2458
SspDI	GGCGCC	1 cut(s)	3355
SspI	AATATT	2 cut(s)	2412, 3601
SspMI	CTAG	4 cut(s)	2459, 2846, 3518, 4017
SstI	GAGCTC	3 cut(s)	139, 243, 274
StyD4I	CCNGG	8 cut(s)	1554, 2205, 2330, 2343, 2629, 2994, 3297, 3924
StyI	CCWWGG	4 cut(s)	2068, 2598, 3435, 3834
TaiI	ACGT	3 cut(s)	26, 785, 3792
TatI	WGTACW	8 cut(s)	1212, 1377, 1632, 2527, 2541, 2639, 3640, 4349
TauI	GCSGC	4 cut(s)	225, 228, 231, 1249
TseFI	GTSAC	4 cut(s)	745, 1690, 1904, 3019
Tsp45I	GTSAC	4 cut(s)	745, 1690, 1904, 3019
TspGWI	ACGGA	5 cut(s)	34, 711, 726, 800, 4319
Van91I	CCANNNNNTGG	2 cut(s)	3501, 3782
Vha464I	CTTAAG	1 cut(s)	2438
VneI	GTGCAC	2 cut(s)	1141, 1666
VpaK11BI	GGWCC	2 cut(s)	1028, 1846
VspI	ATTAAT	1 cut(s)	3864
XapI	RAATTY	6 cut(s)	1767, 2805, 3048, 3063, 3123, 4515
XbaI	TCTAGA	1 cut(s)	3517
XceI	RCATGY	1 cut(s)	4350
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3469, 3502
XhoI	CTCGAG	3 cut(s)	205, 1501, 4457
XmiI	GTMKAC	1 cut(s)	2340
XmnI	GAANNNNTTC	2 cut(s)	479, 2047
XspI	CTAG	4 cut(s)	2459, 2846, 3518, 4017
ZrmI	AGTACT	1 cut(s)	2641

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.