Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005776

Physical Location & Seq

Genomic Coordinates Reverse (-)

42604340 .. 42611957

Gene Structure 7618 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1313800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4530 bp

ATGGCTTCCGGAACTTGGATCACGTCCTTTTCATGTTCGCCATCCGTGGTTCAGTCCTCGGAGGACACCGCCGTAACACCAATCGTTCAATGGCTGAGATTCATTTTCCTCTCTCCCTGTCCTCAGAGAGCTCTGCTGTCCTCTCTCAACCTCCTCTTCTTGCTCTCGCTCTTAGCATTTTCGATACAAAAGCTTTATTCCCGCTTCATCTCCGCGGATGGCGGCCGCAACTCTGACCTCAACAAGCCCCTCATTAGGAACAACAGAGCTCATCTCAGAACTACTCTTTGGTTTAAGCTTTCTCTGATTGTGAGCACTGTGTTGACATTTTGTTATGCAGTTGTTTGCATTCTAGCTTTCACCAGAAGCACCGAGTTGCAATGGAAGCTCGTAGACGGGTTTTTTTGGTTGGTTCAGGCCATAACTCATGCAGTGATCGCAATGCTGGTGGCGCACGAGAGGAGATTTGAAGTGGTTAAGCACCCGCTTTCCCTTCGGATTTATTGGGTCGCAAACTTTGTTGTTATTTCCTTGTTTACAGCATCGGGGATACTTCGTTTGGTTCATGCTCAAGGAATTCAGGAGCCTAGTCTGAGATTGGATGATATAGTTTCAATGGCGTCTTTCCCTTCAGCGATTCTTCTTTTTGTTATCGCCATTAGAGGATCAACTGGGATTGCTGTGAACAGAGAATTTGAGGGAGCTACAAATGGAGATGCGGTATTATACGAACCCCTTTCAAGCAAATCCAACGTGACTGGCTTTGCTTCGGCTTCCATAATTTCCAAGACCTTTTGGATTTGGATGAATCCTCTGCTCAGTAAGGGGTACAAAAGCCCGCTGAAGGTTGACGAAGTCCCATCCCTTTCCCCGGAACATAGAGCTGAAGTAATGTCAATTCTCTTTGAATCGAAGTGGCCAAAACCGCAAGAAAAATTGGACCACCCAGTTCGAACAACATTACTCCGGTGCTTCTGGAAAGAAATTGCTTTCACAGCTTTCCTTGCTGTAATCAGGCTTTGTGTCATGTATGTTGGGCCTCTGCTTATCCAAAGTTTTGTAGATTACACCGCGGGAATAAGAACCTCAGCCTATGAAGGATACTACCTCGTATTGATCCTTCTATGTGCGAAATTCGTTGAAGTTTTAACCACACACCAATTTAACTTCAACTCCCAGAAGCTTGGGATGCTCATCCGTTCCACCCTTATTACTTCTTTGTACAAGAAGGGTTTGAGGTTGTCGTGCTCAGCTAGGCAGGATCATGGAGTTGGACAGATTGTGAATTACATGGCGGTTGATGCCCAACAGCTCTCAGACATGATGCTGCAGCTGCACGCTATATGGATGATGCCAGTGCAACTTACCATTGCGTTGGTGCTTCTATATAACAGCCTTGGTGCAGCAGTAATCACAGCTATAACCGGAATCATGTGTATTCTCGTGTTCGTTGTGCTGGGAACGAAGCGGAACAACAGGTTCCAGTTTAATGTGATGAAGAATCGGGATTCAAGGATGAAGGCAACAAACGAGATGCTTAATTACATGCGCGTGATCAAGTTCCAAGCTTGGGAGGAGCACTTTAACAAAAGGATCCAAGCTTTCCGGGAGTCAGAGTTTAGCTGGCTGACCAAGTTCTTTTACTCAATCTCTGCCAATATCGTGATTATGTGGTGCACCCCAGTACTTATATCAACTCTCACGTTCGGGACTGCACTTTTGTTGGGTGTACGACTTGATGCTGGGACAGTGTTCACAACCACATCCATCTTCAAAATTCTTCAGGAGCCAATCAGGACATTCCCGCAATCTATGATATCAATTTCTCAAGCTATGATATCACTTGGGAGGCTGGATCGTTTTATGAAGAGTAGGGAATTGTTGGAGGATTCGGTGGAGAGGGTAGAAGGCGGTGATAGTGGGGTTGCTGTTGAAGTTAGAGATGGGGTGTTCAACTGGGACGATGAAAGCAATGAAGAGGATTTGAAAAATATAAATATAAAAGTTAATAAAGGTGAGCTAACGGCGATTGTAGGGACAGTGGGATCCGGAAAGTCTTCCCTTCTAGCTTCAATTCTTGGTGAGATGCACAAGTTATCCGGAAAGGTACGAGTTTGCGGGACAACTGCCTATGTGGCTCAAACGTCATGGATTCAAAACGGAACCATTGAAGAAAACATCCTATTTGGTTTGCCAATGGACAGAGAGAGATACCGGGAAGTAATCAAGGTTTGTTGCTTGGAAAAGGACATGGAAATGATGGAGTTCGGAGATCAAACTGAGATAGGAGAGCGTGGCATCAACCTAAGTGGTGGCCAGAAACAGAGGATTCAACTTGCTCGTGCTGTGTATCAAAATTGTGACATATATCTCCTCGATGACGTCTTCAGTGCTGTTGATGCTCATACTGGATCAGAAATATTTAAGGAATGTGTGAGGGGAGCACTTAAAAACAAGACGGTTTTACTTGTAACTCATCAAGTTGACTTCTTGCATAATGTTGATCTTATTTTGGTCATGAGAGATGGGATGATAGTACAGGCAGGAAAGTATAACGATTTGCTAAACTCTGGCCTGGATTTCAAAGAGCTTGTTGCTGCCCACGATACCTCCATGGAACTTGTAGAAATGAGCACTGCTGATCCCAGCAAAAGTTCCCCTGGACATCAAATATCTCGGCAACTCTCCTCAAAACGCGGGGAAGGAAATGGTGAAAACAACTCTTTGGACCAGCTGAGGTCTGATAAAGCGACTTCTAAGCTCATCAAAGAAGAGGAGAAAGAAACCGGCAAAGTCAGCCTTCACATTTACAAAGTCTATTGCACAGAGGCTTACGGATGGTGGGGTGTGGTGTTAGTATTGTCATTGACTCTGATGTGGCAAGCGTCTCAAATGGCGGCTGATTACTGGCTGTCTTATGAAACTGCAGCAAAGCGTGCGGCGTCATTCAAACCATCTGTGTTTATTATCGTCTATGCGCTCATTGCATGTGTCGCATTCGTTGCGGTATTGATAAGAGCACTTGTTGTGATGTTTGTGGGGCTTAAAACAGCTCAAACATTTTTCAAGCAAATTCTTCACAGCATCCTGCATGCTCCCATGTCGTTTTTCGATACTACACCTTCCGGAAGAATTCTGAGTAGGGCATCAACTGATCAGACGAATATCGATCTACTTCTTCCCTTCATGTTGGGTATTACCATTGTCATGTTTTTTACTGTGTTTGGCATCTTTATCATTGTTTGCCAATATTCGTGGCCCACTGTGTTCTTGCTGATTCCGCTTGCTTGGCTTAATGTCTGGTACCGGGGTTACTATCTTGCATCATCTCGAGAGTTAACTCGACTCGATTCAATCACGAAAGCTCCTGTAATCCATCATTTCTCAGAGAGCATATCTGGGGTCATGACGATTCGCTCTTTCAGAATGCAAAACATGTTCTCCAAGGAAAATGTTAAACGAGTTAATGCCAACTTGCGCATGGATTTCCACAACAATGGTTCCAATGAGTGGTTGGGTTTCCGATTGGAGTTACTTGGGAGTTTACTTCTCTGCATTTCCACTATATTCATGATCTTGTTGCCAAGCAGTATTATAAAGCCTGAGAATGTTGGTTTAACTCTCTCCTACGGATTGTCCCTTAATAGTGTGCTGTTCTGGGCTATATATTTTAGCTGCTTCGTTGAAAATAGGATGGTGTCGGTTGAGAGGATAAAACAGTTTACAAACATTCCATCAGAAGCAGCGTGGAAGATTGTAGACCGTGTTCCTCCTCCAAATTGGCCTTCCCGAGGCAACGTTGAGCTCAAAGACTTGCAGGTCAGATATCGTCCAAATACACCTTTGGTTCTCAAAGGCATTAGTCTAAACATTTTCGGGGGTGAGAAGATTGGCGTTGTTGGTCGAACAGGAAGTGGGAAATCCACTTTAATCCAAGTGTTCTTTAGGCTGGTAGAACCTTCAGGAGGCAGAATTATCATCGATGGGATCGATATAACCACGATAGGGCTTCACGATCTTAGGTCACGCTTTGGGATCATTCCTCAAGAACCTGTCCTTTTTGAAGGAACCGTGAGAAGCAACATTGATCCAATTGGAATGTATTCAGATGAACAGATTTGGAAGAGCCTCGAACGCTGCCAACTTAAAGACGTGGTTGCTGCAAAACCAGAAAAACTTAACTGTTTAGTGGCTGACAACGGAGACAATTGGAGCGTGGGGCAAAGACAACTCCTCTGCTTGGGGCGAGTTATGTTAAAGCACAGCCGGCTCTTGTTCATGGACGAGGCCACTGCATCAGTGGATTCACAGACCGATGCTGTAATACAAAGGATCATCCGGGAGGATTTCGCTGCCTGTACAATTATCAGCATTGCGCACAGAATACCTACAGTGATGGACTGCAATCGAGTTTTAGTAGTAGATGCAGGACTGGCTAAGGAATTTGAGAAACCATCCCGCTTACTTGAAAAACCGTCACTGTTCGGTGCGTTGGTTCAGGAGTACGCCAACCGGTCATCAGGACTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006855 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009624 GO:0009628 GO:0009987 GO:0010035 GO:0010118 GO:0012505 GO:0015399 GO:0015405 GO:0015893 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0030054 GO:0031090 GO:0042221 GO:0042493 GO:0042623 GO:0042626 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04071 ko04976 ko04977 ko05206 map01523 map01524 map02010 map04071 map04976 map04977 map05206

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1510

Amino Acids

169.5

Weight (kDa)

8.77

Isoelectric Point (pI)

33.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	331 - 597	7.4e-27	ABC transporter transmembrane region
ABC_tran	PF00005	662 - 795	5.8e-21	ABC transporter
ABC_membrane	PF00664	962 - 1208	1.8e-30	ABC transporter transmembrane region
ABC_tran	PF00005	1284 - 1432	4e-26	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47800 AT3G62700
fragaria_vesca	FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570
malus_domestica	MD03G1286300.v1.1 MD11G1313300.v1.1 MD11G1313800.v1.1 MD11G1314300.v1.1
prunus_persica	Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1
pyrus_communis	pycom03g22680 pycom03g22690 pycom11g27270
rosa_chinensis	RchiOBHm_Chr6g0293781
rosa_laevigata	RLG00000011924
rosa_multiflora	Rmu_sc0005641.1_g000002
rosa_roxburghii	Rroxscaffold_7G00173910
rosa_rugosa	Rorug06G0238000
rosa_samantha	Rh6AG351300 Rh6BG358200 Rh6DG352000
rosa_wichuraiana	Rw6G030560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3596
AasI	GACNNNNNNGTC	1 cut(s)	3818
AatII	GACGTC	1 cut(s)	2385
Acc16I	TGCGCA	2 cut(s)	3479, 4377
Acc36I	ACCTGC	1 cut(s)	3808
Acc65I	GGTACC	1 cut(s)	3303
AccB1I	GGYRCC	1 cut(s)	3303
AccB7I	CCANNNNNTGG	1 cut(s)	3510
AccI	GTMKAC	2 cut(s)	393, 3759
AccII	CGCG	4 cut(s)	215, 1073, 1551, 2697
AccIII	TCCGGA	4 cut(s)	8, 2048, 2099, 3125
AclI	AACGTT	1 cut(s)	3798
AcoI	YGGCCR	3 cut(s)	223, 917, 2314
AcsI	RAATTY	7 cut(s)	576, 692, 1133, 1776, 3072, 3132, 4442
AcuI	CTGAAG	6 cut(s)	615, 863, 906, 1766, 2371, 3945
AcyI	GRCGYC	3 cut(s)	620, 2382, 2942
AfaI	GTAC	9 cut(s)	830, 1223, 1686, 1731, 2109, 2538, 3305, 4360, 4505
AflIII	ACRYGT	1 cut(s)	3435
AgeI	ACCGGT	1 cut(s)	4512
AjiI	CACGTC	2 cut(s)	24, 4153
AjnI	CCWGG	2 cut(s)	2574, 2659
AjuI	GAANNNNNNNTTGG	4 cut(s)	271, 303, 3769, 3801
Alw26I	GTCTC	2 cut(s)	2892, 4197
Alw44I	GTGCAC	1 cut(s)	1675
Ama87I	CYCGRG	2 cut(s)	3330, 3789
Aor13HI	TCCGGA	4 cut(s)	8, 2048, 2099, 3125
AoxI	GGCC	9 cut(s)	223, 417, 917, 1037, 2314, 2572, 3257, 3782, 4287
ApaLI	GTGCAC	1 cut(s)	1675
ApoI	RAATTY	7 cut(s)	576, 692, 1133, 1776, 3072, 3132, 4442
ArsI	GACNNNNNNTTYG	2 cut(s)	1702, 1734
AsiGI	ACCGGT	1 cut(s)	4512
Asp700I	GAANNNNTTC	2 cut(s)	2056, 4102
Asp718I	GGTACC	1 cut(s)	3303
AspLEI	GCGC	5 cut(s)	454, 1551, 2980, 3480, 4378
AspS9I	GGNCC	4 cut(s)	940, 1037, 2728, 3258
AsuC2I	CCSGG	5 cut(s)	872, 1607, 2216, 3308, 4340
AsuHPI	GGTGA	6 cut(s)	352, 1925, 2027, 2093, 2723, 3893
AsuII	TTCGAA	1 cut(s)	952
AvaI	CYCGRG	2 cut(s)	3330, 3789
AvaII	GGWCC	2 cut(s)	940, 2728
BaeGI	GKGCMC	1 cut(s)	1679
BaeI	ACNNNNGTAYC	2 cut(s)	3295, 3328
BalI	TGGCCA	2 cut(s)	919, 2316
BamHI	GGATCC	2 cut(s)	1593, 2045
BanI	GGYRCC	1 cut(s)	3303
BanII	GRGCYC	3 cut(s)	133, 271, 3807
BarI	GAAGNNNNNNTAC	2 cut(s)	1104, 1136
BauI	CACGAG	3 cut(s)	455, 1442, 2340
BbsI	GAAGAC	2 cut(s)	2049, 2377
BbvCI	CCTCAGC	2 cut(s)	1087, 2735
BceAI	ACGGC	2 cut(s)	56, 2040
BcgI	CGANNNNNNTGC	2 cut(s)	4274, 4308
BciT130I	CCWGG	2 cut(s)	2576, 2661
BciVI	GTATCC	2 cut(s)	543, 1094
BclI	TGATCA	2 cut(s)	1554, 3154
BcnI	CCSGG	5 cut(s)	872, 1607, 2216, 3308, 4340
BcoDI	GTCTC	2 cut(s)	2892, 4197
BfaI	CTAG	4 cut(s)	353, 588, 1254, 2066
BfmI	CTRYAG	3 cut(s)	1328, 2925, 4389
BfuAI	ACCTGC	1 cut(s)	3808
BfuI	GTATCC	2 cut(s)	543, 1094
BglI	GCCNNNNNGGC	1 cut(s)	2135
BlpI	GCTNAGC	1 cut(s)	1249
BmcAI	AGTACT	1 cut(s)	1686
Bme1390I	CCNGG	7 cut(s)	872, 1607, 2216, 2576, 2661, 3308, 4340
Bme18I	GGWCC	2 cut(s)	940, 2728
BmeT110I	CYCGRG	2 cut(s)	3330, 3789
BmgBI	CACGTC	2 cut(s)	24, 4153
BmgT120I	GGNCC	4 cut(s)	940, 1037, 2728, 3258
BmiI	GGNNCC	9 cut(s)	585, 1481, 1595, 1788, 2047, 2164, 3305, 3502, 4069
BmrFI	CCNGG	7 cut(s)	872, 1607, 2216, 2576, 2661, 3308, 4340
BmrI	ACTGGG	4 cut(s)	681, 941, 1676, 1966
BmuI	ACTGGG	4 cut(s)	681, 941, 1676, 1966
BpiI	GAAGAC	2 cut(s)	2049, 2377
BplI	GAGNNNNNCTC	2 cut(s)	258, 290
Bpu10I	CCTNAGC	3 cut(s)	1087, 2735, 4437
Bpu1102I	GCTNAGC	1 cut(s)	1249
Bpu14I	TTCGAA	1 cut(s)	952
BpuEI	CTTGAG	3 cut(s)	555, 1812, 4029
BpuMI	CCSGG	5 cut(s)	872, 1607, 2216, 3308, 4340
Bsa29I	ATCGAT	3 cut(s)	3168, 3981, 3990
BsaBI	GATNNNNATC	1 cut(s)	1193
BsaHI	GRCGYC	3 cut(s)	620, 2382, 2942
BsaWI	WCCGGW	7 cut(s)	8, 966, 1424, 2048, 2099, 3125, 4512
Bse118I	RCCGGY	3 cut(s)	2786, 4266, 4512
Bse3DI	GCAATG	6 cut(s)	386, 447, 1368, 1978, 2982, 4371
Bse8I	GATNNNNATC	1 cut(s)	1193
BseAI	TCCGGA	4 cut(s)	8, 2048, 2099, 3125
BseBI	CCWGG	2 cut(s)	2576, 2661
BseCI	ATCGAT	3 cut(s)	3168, 3981, 3990
BseJI	GATNNNNATC	1 cut(s)	1193
BseMI	GCAATG	6 cut(s)	386, 447, 1368, 1978, 2982, 4371
BseRI	GAGGAG	8 cut(s)	143, 475, 1589, 2363, 2677, 2789, 3762, 4223
BseSI	GKGCMC	1 cut(s)	1679
BseX3I	CGGCCG	1 cut(s)	223
BseYI	CCCAGC	3 cut(s)	1456, 1742, 2645
BsgI	GTGCAG	3 cut(s)	1319, 1422, 1698
Bsh1236I	CGCG	4 cut(s)	215, 1073, 1551, 2697
Bsh1285I	CGRYCG	1 cut(s)	226
BshFI	GGCC	9 cut(s)	225, 419, 919, 1039, 2316, 2574, 3259, 3784, 4289
BshNI	GGYRCC	1 cut(s)	3303
BshTI	ACCGGT	1 cut(s)	4512
BshVI	ATCGAT	3 cut(s)	3168, 3981, 3990
BsiEI	CGRYCG	1 cut(s)	226
BsiHKCI	CYCGRG	2 cut(s)	3330, 3789
BslFI	GGGAC	7 cut(s)	842, 1723, 1759, 1973, 2050, 2134, 3622
BsmAI	GTCTC	2 cut(s)	2892, 4197
BsmBI	CGTCTC	1 cut(s)	2892
BsmFI	GGGAC	7 cut(s)	842, 1723, 1759, 1973, 2050, 2134, 3622
BsmI	GAATGC	3 cut(s)	348, 2996, 3432
BsnI	GGCC	9 cut(s)	225, 419, 919, 1039, 2316, 2574, 3259, 3784, 4289
BsoBI	CYCGRG	2 cut(s)	3330, 3789
Bsp119I	TTCGAA	1 cut(s)	952
Bsp13I	TCCGGA	4 cut(s)	8, 2048, 2099, 3125
Bsp1407I	TGTACA	2 cut(s)	1221, 4358
Bsp1720I	GCTNAGC	1 cut(s)	1249
Bsp19I	CCATGG	1 cut(s)	2613
BspANI	GGCC	9 cut(s)	225, 419, 919, 1039, 2316, 2574, 3259, 3784, 4289
BspDI	ATCGAT	3 cut(s)	3168, 3981, 3990
BspEI	TCCGGA	4 cut(s)	8, 2048, 2099, 3125
BspFNI	CGCG	4 cut(s)	215, 1073, 1551, 2697
BspHI	TCATGA	3 cut(s)	2517, 3405, 3570
BspLI	GGNNCC	9 cut(s)	585, 1481, 1595, 1788, 2047, 2164, 3305, 3502, 4069
BspMAI	CTGCAG	2 cut(s)	1332, 2929
BspMI	ACCTGC	1 cut(s)	3808
BspQI	GCTCTTC	1 cut(s)	4118
BspT104I	TTCGAA	1 cut(s)	952
BspT107I	GGYRCC	1 cut(s)	3303
BsrDI	GCAATG	6 cut(s)	386, 447, 1368, 1978, 2982, 4371
BsrFI	RCCGGY	3 cut(s)	2786, 4266, 4512
BsrGI	TGTACA	2 cut(s)	1221, 4358
BssAI	RCCGGY	3 cut(s)	2786, 4266, 4512
BssNI	GRCGYC	3 cut(s)	620, 2382, 2942
BssSI	CACGAG	3 cut(s)	455, 1442, 2340
BssT1I	CCWWGG	3 cut(s)	1396, 2613, 3444
Bst2BI	CACGAG	3 cut(s)	455, 1442, 2340
Bst2UI	CCWGG	2 cut(s)	2576, 2661
Bst6I	CTCTTC	5 cut(s)	161, 1862, 1971, 2766, 4118
BstACI	GRCGYC	3 cut(s)	620, 2382, 2942
BstAPI	GCANNNNNTGC	2 cut(s)	2936, 3002
BstAUI	TGTACA	2 cut(s)	1221, 4358
BstBI	TTCGAA	1 cut(s)	952
BstC8I	GCNNGC	8 cut(s)	839, 1338, 1625, 2883, 2937, 3093, 3285, 4268
BstDSI	CCRYGG	4 cut(s)	45, 213, 1071, 2613
BstENI	CCTNNNNNAGG	2 cut(s)	3789, 3963
BstFNI	CGCG	4 cut(s)	215, 1073, 1551, 2697
BstHHI	GCGC	5 cut(s)	454, 1551, 2980, 3480, 4378
BstMAI	GTCTC	2 cut(s)	2892, 4197
BstMCI	CGRYCG	1 cut(s)	226
BstNI	CCWGG	2 cut(s)	2576, 2661
BstNSI	RCATGY	4 cut(s)	1549, 2991, 3095, 3439
BstSCI	CCNGG	7 cut(s)	870, 1605, 2214, 2574, 2659, 3306, 4338
BstSFI	CTRYAG	3 cut(s)	1328, 2925, 4389
BstSLI	GKGCMC	1 cut(s)	1679
BstUI	CGCG	4 cut(s)	215, 1073, 1551, 2697
BstV2I	GAAGAC	2 cut(s)	2049, 2377
BstX2I	RGATCY	2 cut(s)	1593, 2045
BstXI	CCANNNNNNTGG	3 cut(s)	1184, 1375, 3497
BstYI	RGATCY	2 cut(s)	1593, 2045
BstZI	CGGCCG	1 cut(s)	223
Bsu15I	ATCGAT	3 cut(s)	3168, 3981, 3990
BsuI	GTATCC	2 cut(s)	543, 1094
BsuRI	GGCC	9 cut(s)	225, 419, 919, 1039, 2316, 2574, 3259, 3784, 4289
BsuTUI	ATCGAT	3 cut(s)	3168, 3981, 3990
BtgI	CCRYGG	4 cut(s)	45, 213, 1071, 2613
BtrI	CACGTC	2 cut(s)	24, 4153
BtsI	GCAGTG	3 cut(s)	438, 2634, 4290
BveI	ACCTGC	1 cut(s)	3808
Cac8I	GCNNGC	8 cut(s)	839, 1338, 1625, 2883, 2937, 3093, 3285, 4268
CciI	TCATGA	3 cut(s)	2517, 3405, 3570
CciNI	GCGGCCGC	1 cut(s)	223
CfoI	GCGC	5 cut(s)	454, 1551, 2980, 3480, 4378
Cfr10I	RCCGGY	3 cut(s)	2786, 4266, 4512
Cfr13I	GGNCC	4 cut(s)	940, 1037, 2728, 3258
Cfr42I	CCGCGG	2 cut(s)	216, 1074
ClaI	ATCGAT	3 cut(s)	3168, 3981, 3990
CseI	GACGC	3 cut(s)	609, 2874, 2931
Csp6I	GTAC	9 cut(s)	829, 1222, 1685, 1730, 2108, 2537, 3304, 4359, 4504
CspAI	ACCGGT	1 cut(s)	4512
CspCI	CAANNNNNGTGG	6 cut(s)	429, 464, 2290, 2325, 3236, 3271
CviQI	GTAC	9 cut(s)	829, 1222, 1685, 1730, 2108, 2537, 3304, 4359, 4504
DrdI	GACNNNNNNGTC	1 cut(s)	3818
DseDI	GACNNNNNNGTC	1 cut(s)	3818
EaeI	YGGCCR	3 cut(s)	223, 917, 2314
EagI	CGGCCG	1 cut(s)	223
Eam1104I	CTCTTC	5 cut(s)	161, 1862, 1971, 2766, 4118
EarI	CTCTTC	5 cut(s)	161, 1862, 1971, 2766, 4118
Ecl136II	GAGCTC	3 cut(s)	131, 269, 3805
EclXI	CGGCCG	1 cut(s)	223
Eco130I	CCWWGG	3 cut(s)	1396, 2613, 3444
Eco24I	GRGCYC	3 cut(s)	133, 271, 3807
Eco32I	GATATC	3 cut(s)	1818, 1839, 3827
Eco47I	GGWCC	2 cut(s)	940, 2728
Eco52I	CGGCCG	1 cut(s)	223
Eco53kI	GAGCTC	3 cut(s)	131, 269, 3805
Eco57I	CTGAAG	6 cut(s)	615, 863, 906, 1766, 2371, 3945
Eco88I	CYCGRG	2 cut(s)	3330, 3789
EcoICRI	GAGCTC	3 cut(s)	131, 269, 3805
EcoNI	CCTNNNNNAGG	2 cut(s)	3789, 3963
EcoRI	GAATTC	2 cut(s)	576, 3132
EcoRII	CCWGG	2 cut(s)	2574, 2659
EcoRV	GATATC	3 cut(s)	1818, 1839, 3827
EcoT14I	CCWWGG	3 cut(s)	1396, 2613, 3444
EcoT38I	GRGCYC	3 cut(s)	133, 271, 3807
ErhI	CCWWGG	3 cut(s)	1396, 2613, 3444
Esp3I	CGTCTC	1 cut(s)	2892
FalI	AAGNNNNNCTT	2 cut(s)	2784, 2816
FaqI	GGGAC	7 cut(s)	842, 1723, 1759, 1973, 2050, 2134, 3622
FauI	CCCGC	8 cut(s)	209, 492, 846, 1066, 1812, 2111, 2690, 4466
FbaI	TGATCA	2 cut(s)	1554, 3154
FblI	GTMKAC	2 cut(s)	393, 3759
FriOI	GRGCYC	3 cut(s)	133, 271, 3807
FspBI	CTAG	4 cut(s)	353, 588, 1254, 2066
FspI	TGCGCA	2 cut(s)	3479, 4377
GlaI	GCGC	5 cut(s)	453, 1550, 2979, 3479, 4377
GsaI	CCCAGC	3 cut(s)	1460, 1746, 2649
HaeIII	GGCC	9 cut(s)	225, 419, 919, 1039, 2316, 2574, 3259, 3784, 4289
HgaI	GACGC	3 cut(s)	609, 2874, 2931
HhaI	GCGC	5 cut(s)	454, 1551, 2980, 3480, 4378
Hin1I	GRCGYC	3 cut(s)	620, 2382, 2942
Hin6I	GCGC	5 cut(s)	452, 1549, 2978, 3478, 4376
HinP1I	GCGC	5 cut(s)	452, 1549, 2978, 3478, 4376
HincII	GTYRAC	4 cut(s)	324, 850, 2485, 3339
HindII	GTYRAC	4 cut(s)	324, 850, 2485, 3339
HindIII	AAGCTT	5 cut(s)	191, 296, 1181, 1566, 1599
HpaI	GTTAAC	1 cut(s)	3339
HphI	GGTGA	6 cut(s)	352, 1925, 2027, 2093, 2723, 3893
HpyCH4IV	ACGT	7 cut(s)	23, 753, 1703, 2144, 2382, 3798, 4152
HpySE526I	ACGT	7 cut(s)	23, 753, 1703, 2144, 2382, 3798, 4152
Hsp92I	GRCGYC	3 cut(s)	620, 2382, 2942
HspAI	GCGC	5 cut(s)	452, 1549, 2978, 3478, 4376
Kpn2I	TCCGGA	4 cut(s)	8, 2048, 2099, 3125
KpnI	GGTACC	1 cut(s)	3307
KroI	GCCGGC	1 cut(s)	4266
KroNI	GCCGGC	1 cut(s)	4268
Ksp22I	TGATCA	2 cut(s)	1554, 3154
KspAI	GTTAAC	1 cut(s)	3339
KspI	CCGCGG	2 cut(s)	216, 1074
LguI	GCTCTTC	1 cut(s)	4118
LmnI	GCTCC	8 cut(s)	583, 701, 1576, 1786, 2441, 3100, 3370, 4212
MaeI	CTAG	4 cut(s)	353, 588, 1254, 2066
MaeII	ACGT	7 cut(s)	23, 753, 1703, 2144, 2382, 3798, 4152
MaeIII	GTNAC	8 cut(s)	73, 754, 2360, 2470, 3311, 3531, 4023, 4476
MfeI	CAATTG	2 cut(s)	4092, 4207
MflI	RGATCY	2 cut(s)	1593, 2045
MlsI	TGGCCA	2 cut(s)	919, 2316
MluNI	TGGCCA	2 cut(s)	919, 2316
MlyI	GAGTC	3 cut(s)	1619, 2863, 3339
MmeI	TCCRAC	3 cut(s)	774, 1252, 1863
Mox20I	TGGCCA	2 cut(s)	919, 2316
MroI	TCCGGA	4 cut(s)	8, 2048, 2099, 3125
MroNI	GCCGGC	1 cut(s)	4266
MroXI	GAANNNNTTC	2 cut(s)	2056, 4102
MscI	TGGCCA	2 cut(s)	919, 2316
MslI	CAYNNNNRTG	4 cut(s)	1550, 2255, 3206, 3495
Msp20I	TGGCCA	2 cut(s)	919, 2316
MspA1I	CMGCKG	5 cut(s)	215, 841, 1073, 1333, 2734
MspR9I	CCNGG	7 cut(s)	872, 1607, 2216, 2576, 2661, 3308, 4340
MunI	CAATTG	2 cut(s)	4092, 4207
Mva1269I	GAATGC	3 cut(s)	348, 2996, 3432
MvaI	CCWGG	2 cut(s)	2576, 2661
MvnI	CGCG	4 cut(s)	215, 1073, 1551, 2697
NaeI	GCCGGC	1 cut(s)	4268
NciI	CCSGG	5 cut(s)	872, 1607, 2216, 3308, 4340
NcoI	CCATGG	1 cut(s)	2613
NgoMIV	GCCGGC	1 cut(s)	4266
NlaIV	GGNNCC	9 cut(s)	585, 1481, 1595, 1788, 2047, 2164, 3305, 3502, 4069
NmeAIII	GCCGAG	1 cut(s)	2656
NmuCI	GTSAC	4 cut(s)	754, 2360, 4023, 4476
NotI	GCGGCCGC	1 cut(s)	223
NsbI	TGCGCA	2 cut(s)	3479, 4377
NspI	RCATGY	4 cut(s)	1549, 2991, 3095, 3439
NspV	TTCGAA	1 cut(s)	952
PaeI	GCATGC	1 cut(s)	3095
PaeR7I	CTCGAG	1 cut(s)	3330
PagI	TCATGA	3 cut(s)	2517, 3405, 3570
PciI	ACATGT	1 cut(s)	3435
PciSI	GCTCTTC	1 cut(s)	4118
PcsI	WCGNNNNNNNCGW	1 cut(s)	3987
PctI	GAATGC	3 cut(s)	348, 2996, 3432
PdiI	GCCGGC	1 cut(s)	4268
PdmI	GAANNNNTTC	2 cut(s)	2056, 4102
PflFI	GACNNNGTC	1 cut(s)	854
PflMI	CCANNNNNTGG	1 cut(s)	3510
PfoI	TCCNGGA	2 cut(s)	1605, 4338
PinAI	ACCGGT	1 cut(s)	4512
PleI	GAGTC	3 cut(s)	1618, 2863, 3339
PpsI	GAGTC	3 cut(s)	1618, 2863, 3339
PscI	ACATGT	1 cut(s)	3435
PsiI	TTATAA	1 cut(s)	3596
Psp124BI	GAGCTC	3 cut(s)	133, 271, 3807
Psp1406I	AACGTT	1 cut(s)	3798
Psp6I	CCWGG	2 cut(s)	2574, 2659
PspFI	CCCAGC	3 cut(s)	1456, 1742, 2645
PspGI	CCWGG	2 cut(s)	2574, 2659
PspN4I	GGNNCC	9 cut(s)	585, 1481, 1595, 1788, 2047, 2164, 3305, 3502, 4069
PspPI	GGNCC	4 cut(s)	940, 1037, 2728, 3258
PsrI	GAACNNNNNNTAC	2 cut(s)	3750, 3782
PstI	CTGCAG	2 cut(s)	1332, 2929
PsuI	RGATCY	2 cut(s)	1593, 2045
PsyI	GACNNNGTC	1 cut(s)	854
PvuII	CAGCTG	2 cut(s)	1333, 2734
RsaI	GTAC	9 cut(s)	830, 1223, 1686, 1731, 2109, 2538, 3305, 4360, 4505
RsaNI	GTAC	9 cut(s)	829, 1222, 1685, 1730, 2108, 2537, 3304, 4359, 4504
RseI	CAYNNNNRTG	4 cut(s)	1550, 2255, 3206, 3495
SacI	GAGCTC	3 cut(s)	133, 271, 3807
SacII	CCGCGG	2 cut(s)	216, 1074
SapI	GCTCTTC	1 cut(s)	4118
Sau96I	GGNCC	4 cut(s)	940, 1037, 2728, 3258
ScaI	AGTACT	1 cut(s)	1686
SchI	GAGTC	3 cut(s)	1619, 2863, 3339
ScrFI	CCNGG	7 cut(s)	872, 1607, 2216, 2576, 2661, 3308, 4340
SfcI	CTRYAG	3 cut(s)	1328, 2925, 4389
Sfr274I	CTCGAG	1 cut(s)	3330
Sfr303I	CCGCGG	2 cut(s)	216, 1074
SfuI	TTCGAA	1 cut(s)	952
SgrBI	CCGCGG	2 cut(s)	216, 1074
SinI	GGWCC	2 cut(s)	940, 2728
SlaI	CTCGAG	1 cut(s)	3330
SmiMI	CAYNNNNRTG	4 cut(s)	1550, 2255, 3206, 3495
SmlI	CTYRAG	4 cut(s)	570, 1827, 3330, 4044
SmoI	CTYRAG	4 cut(s)	570, 1827, 3330, 4044
SphI	GCATGC	1 cut(s)	3095
SspI	AATATT	2 cut(s)	2421, 3251
SspMI	CTAG	4 cut(s)	353, 588, 1254, 2066
SstI	GAGCTC	3 cut(s)	133, 271, 3807
StyD4I	CCNGG	7 cut(s)	870, 1605, 2214, 2574, 2659, 3306, 4338
StyI	CCWWGG	3 cut(s)	1396, 2613, 3444
TaiI	ACGT	7 cut(s)	26, 756, 1706, 2147, 2385, 3801, 4155
TaqII	GACCGA	1 cut(s)	4328
TatI	WGTACW	4 cut(s)	1221, 1684, 2536, 4358
TauI	GCSGC	4 cut(s)	225, 228, 2900, 2942
TseFI	GTSAC	4 cut(s)	754, 2360, 4023, 4476
Tsp45I	GTSAC	4 cut(s)	754, 2360, 4023, 4476
TspGWI	ACGGA	6 cut(s)	34, 1187, 2175, 2850, 3645, 4215
Tth111I	GACNNNGTC	1 cut(s)	854
Van91I	CCANNNNNTGG	1 cut(s)	3510
VneI	GTGCAC	1 cut(s)	1675
VpaK11BI	GGWCC	2 cut(s)	940, 2728
XagI	CCTNNNNNAGG	2 cut(s)	3789, 3963
XapI	RAATTY	7 cut(s)	576, 692, 1133, 1776, 3072, 3132, 4442
XceI	RCATGY	4 cut(s)	1549, 2991, 3095, 3439
XcmI	CCANNNNNNNNNTGG	4 cut(s)	87, 3478, 3511, 4297
XhoI	CTCGAG	1 cut(s)	3330
XmiI	GTMKAC	2 cut(s)	393, 3759
XmnI	GAANNNNTTC	2 cut(s)	2056, 4102
XspI	CTAG	4 cut(s)	353, 588, 1254, 2066
ZraI	GACGTC	1 cut(s)	2383
ZrmI	AGTACT	1 cut(s)	1686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.