Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005776

Physical Location & Seq

Genomic Coordinates Forward (+)

36584609 .. 36593222

Gene Structure 8614 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1286300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4530 bp

ATGTCTTCCGGGACTTGGATCACATCATTTTCGTGTTCGCCATCCGTGGTTCAGTCCTCGGAGGACACCGCCGTGACACCAATCTTTCAATGGCTGAGATTCATTTTCCTCTCTCCCTGTCCTCAGAGAGCTCTGCTGTCCACTGTCGACCTCCTCTTCTTGCTCTCGCTCTTGGCATTTTCGATACACAAGCTTTATTCCCGCTTCATCTCCGCGGATGGCGGCCGCAACTCCGACCTCAACAAGCCCCTCATTAGGAACAACAGAGCTCATCTCAGAACTACTCTCCTCTTTAAGGCTTCTCTGATGGTGAGCAGTCTGTTGACAATATGTTATGCAGTTGTTTGCATTCTTGCTTTCACTAGAAGCACCGAGTTGCCATGGAAGCTCGTAGACGGGGTTTTCTGGTTGGTTCAGTCCATAACTCATGCAGTGATCACAATTCTGGTGGCGCACGGGAAGAGATTTGAAGCGGTTAAGCACCCGCTTTCTCTTCGGATTTATTGGGTTGCAAACTTTGTGGTTATTTCCTTGTTCACAGCATCGGGGATACTTCGTTTGGTTTATGCTCAAGGAATTCAGGAGCCTAGTTTGAGATTGGATGATATAGTTTCAATGGCTTCTTTCCCTTTGGCGATTGTTCTTCTTGTTATCGCCATTAGAGGATCAACTGGGATTTCTGTGAACAGAGAATTTGAGGGAGTTACAAACGGAGACGCAGTATTATACGAACCCCTTTCGAACAAATCCAATGTGACTGGCTTTGCTTCGGCTTCTATAATTTCTAAAACCTTTTGGATTTGGATGAATCCTCTGCTAAGTAAAGGGTACAAAAGTCCGCTGAAGGTTGACGAAATCCCATCCCTTTCCCCGGAACATAGAGCTGAAGTAATGTCAGTTCTCTTTGAATCGAAGTGGCCAAAACCGCAAGAAAAATCAGACCATCCAGTTCGAACAACATTACTCCGGTGCTTCTGGAAAGAAATTGCTTTCACAGCTTCCCTTGCTGTGATCAGGCTTTGTGTCCTGTATGTTGGGCCTGTGCTTATCCAAAGTTTTGTAGATTACACCGCGGGAATAAGAACCTCGCCATTTGAAGGATACTACCTCGTATTGATCCTTCTATGCGCGAAATTTGTCGAAGTTTTAACCACTCACCAATTTAACTTCAACTCCCAGAAGCTTGGGATGCTCATTCGTTCCACGCTTATTACTTCTTTGTACAAGAAGGGGTTGAGGTTGTCATGCTCAGCTCGGCAGGCTCATGGAGTTGGACAGATTGTGAATTACATGGCGGTTGATGCCCAACAGCTCTCAGATATGATGCTGCAGCTGCACTCTATATGGATGATGCCGGTGCAACTTACCATCGCATTGGCCCTTCTATATAACACCCTTGGTGCAGCAGCGATCACAGCTGTAATCGGAGTCATGTGTATTCTTGTGTTTGTTGTGCTGGGAGCGAGGCGGAACAACAGGTTCAATTTTAATGTGATGAAGAATCGGGATTCAAGGATGAAGGCAACGAACGAGATGCTTAATTATATGCGCGTGATCAAGTTCCAAGCTTGGGAGGATTACTTTAACAAAAGAATCCAAGCTTTCCGCGACTCAGAGTTTAGCTGGCTGACCAAGTTCTTTTACTCAATCAATGCCAATGTCGTGGTTATGTGGTGCACCCCAGTACTTATATCAACTCTCACATTCGGGACTGCACTTTTGTTGGGTGTACAACTCGATGCTGGGACAGTGTTCACAACCACAACCATCTTCAAAATTCTTCAGGAGCCAATCAGGGCATTCCCGCAATCTATGATATCTATTTCTCAAGCTATTATATCGCTTGGGAGGTTGGATCGATATATGATGAGTAGGGAATTGGTGGAGGATTCGGTGGAGAGGGTAGAAGACTGCGATAGTAGGATTGCTGTTGAAGTTAAAGATGCCGCGTTTAGCTGGGACGATGAAAGCAATGAAGAGGCTTTGAAAAACATAAATATAAATGTTAACAAAGGCGAGCTAACCGCGATTGTAGGGACAGTGGGATCCGGAAAGTCTTCCCTTCTCGCCTCAATTCTCGGTGAGATGCACAAGTTATCGGGAACGGTACGAGTTTGCGGGAAAACTGCCTACGTGGCTCAAACATCATGGATTCAAAACGGAACCATTGAAGAAAACATCCTATTTGGTTTTCCAATGGACAGAGAGAGATACCGGGAAGTTGTCAGGGTTTGTTGCTTGGAAAAGGACATGGAACTGATGGAGTTTGGAGATCAAACTGAGATAGGAGAGCGTGGCATCAACCTAAGTGGTGGCCAGAAACAGAGGATACAACTTGCTCGTGCTGTGTATCAAAACTGTGACATATATCTTCTCGATGACGTCTTCAGTGCTGTTGATGCTCATACAGGGTCCGAAATATTTAAGGAATGTGTGAGGGGAGCGCTTAAAAACAAGACGGTTTTACTTGTAACTCATCAAGTGGACTTCTTGCATAATGTTGATCTTATTTTGGTCATGAGAGACGGGATGATAGTACAAGCAGGAAAGTATAACGATTTGCTAAATTCCGGCCTGGATTTCAAAGAGCTTGTTGCTGCCCACGAAAACTCCATGGAACTTGTAGAAATGAGTACTGCTGATCCCAGCAAAAGTTCCCACGGACCTCAAATATCTCAGCAACTCTCTTCAAAACGTGGGGAAGCAAATGGTGAAAACAGCTCTTTGGACCAGCCAAAGTCTGATAAAGCGAGTTCTAAGCTCATCAAAGAAGAGGAGAAAGAAACCGGCAAAGTAAGCTTGCACATTTACAAAGTTTACTGCACGGAGGCTTACGGATGGGGTGGTGTGGTGGTAGTGTTGTCATTGTCTCTGATGTGGCAAGCATCTCAAATGTCGGGTGATTACTGGCTGTCGTATGAAACTGCAGCAAAGCGTGCAGCGTCATTCAAACCATCGGAGTTTATAATCGTCTATGCAATTATTGCTTGTGTCTCATTCGTTGTGGTATTGATAAGAGCACTTGTCGTGATGTTTGTGGGGCTTAAAACAGCTCAGACATTTTTCAAGCAAATTCTTCATAGCATCCTGCATGCCCCCATGTCGTTTTTCGATACTACACCTTCTGGAAGAATTCTGAGTAGGGCATCAACTGATCAGACGAATATCGATCTATTTCTTCCCTTCATAATGGGTGTCACCATTGCCATGTACTTTACTGTGCTTGGCATCTTCATCATCATATGCCAATACTCATGGCCCACTGTATTCTTGCTGATTCCGCTTGTTTGGCTTAATGTCTGGTACCGGGGTTACTATCTTGCATCATCTCGAGAGTTAACTCGACTTGATTCAATCACAAAAGCTCCTGTAATCCATCATTTCTCAGAAAGCATATCTGGGGTGATGACAATCCGCTCATTCAGAATGCAAAGCTTGTTCTCCAAGGAAAACATCAAACGAGTTAATGCCAACTTACGTATGGATTTCCACAACAATGGGTCCAATGAGTGGTTGGGTTTCCGATTGGAGATGCTTGGGAGTTTACTTCTCTGTGTATCCGCTATATTCATGATCTTGTTGCCGAGCAGCATTATAAAGCCCGAGAATGTTGGTTTAACTCTCTCCTACGGATTGTCCCTTAATGGTGTGCTGTTCTGGGCCATATATTTGAGCTGCTTCGTTGAAAATAGGATGGTGTCGGTTGAGAGGGTAAAACAGTTCACAAACATTCCATCAGAAGCAGCATGGAAGATTGTAGACCGTGTTCCTCCTCCAAATTGGCCTTCTCGTGGCAACGTTGAGCTCAAAGACTTGCAGGTCAGATATCGCCCTAATACACCATTGGTTCTGAAAGGCATTAGTTTAAACATTTTCGGGGGTGAGAAGATTGGCGTTGTTGGTCGAACAGGAAGTGGGAAATCCACTATAATCCAAGTGTTCTTTAGGCTGGTAGAACCTTCAGGAGGCAGAATTATCATCGATGGGATCGATATAACCACAATAGGGCTTCACGATCTCAGGTCTCGCTTTGGGATCATTCCTCAAGAACCTGTCCTTTTTGAAGGAACTGTGAGAAGCAACATTGACCCAGTTGGAATGTATTCAGATGAAGAGATTTGGAAGAGCCTCGAACGCTGCCAACTTAAAGACGTGGTTGCTGCAAAACCCGAAAAACTTAACTCTTTAGTGGCTGATAACGGAGACAATTGGAGCGTGGGACAAAGACAGCTTCTCTGTTTGGGACGAGTCATGTTAAAGCACAGCCGACTCTTGTTCATGGACGAGGCCACTGCATCAGTGGATTCACAGACCGATGCTGTAATACAAAGGATCATCCGAGAGGATTTCGCTGCCTGTACAATTATCAGCATTGCGCACAGAATACCAACGGTGATGGACTGCAATCGAGTTTTAGTAGTAGATGCAGGACTAGCTAGGGAATTTGATGCACCATCCCGCTTGCTTGAAAGACCGTCACTGTTTGGTGCCTTGGTTCAGGAGTACGCCAACCGATCATCAGGACTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006855 GO:0006950 GO:0008150 GO:0009414 GO:0009415 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009624 GO:0009628 GO:0009987 GO:0010035 GO:0010118 GO:0012505 GO:0015399 GO:0015405 GO:0015893 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0030054 GO:0031090 GO:0042221 GO:0042493 GO:0042623 GO:0042626 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04071 ko04976 ko04977 ko05206 map01523 map01524 map02010 map04071 map04976 map04977 map05206

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1510

Amino Acids

168.87

Weight (kDa)

8.55

Isoelectric Point (pI)

36.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	335 - 597	3.1e-26	ABC transporter transmembrane region
ABC_tran	PF00005	662 - 795	3.3e-21	ABC transporter
ABC_membrane	PF00664	983 - 1209	1e-30	ABC transporter transmembrane region
ABC_tran	PF00005	1284 - 1432	6e-26	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47800 AT3G62700
fragaria_vesca	FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570 FvH4_2g25570
malus_domestica	MD03G1286300.v1.1 MD11G1313300.v1.1 MD11G1313800.v1.1 MD11G1314300.v1.1
prunus_persica	Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1 Prupe.8G259800_v2.0.a1
pyrus_communis	pycom03g22680 pycom03g22690 pycom11g27270
rosa_chinensis	RchiOBHm_Chr6g0293781
rosa_laevigata	RLG00000011924
rosa_multiflora	Rmu_sc0005641.1_g000002
rosa_roxburghii	Rroxscaffold_7G00173910
rosa_rugosa	Rorug06G0238000
rosa_samantha	Rh6AG351300 Rh6BG358200 Rh6DG352000
rosa_wichuraiana	Rw6G030560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2966, 3596
AasI	GACNNNNNNGTC	1 cut(s)	3818
AatII	GACGTC	1 cut(s)	2385
Acc16I	TGCGCA	1 cut(s)	4377
Acc36I	ACCTGC	1 cut(s)	3808
Acc65I	GGTACC	1 cut(s)	3303
AccB1I	GGYRCC	2 cut(s)	3303, 4487
AccB7I	CCANNNNNTGG	1 cut(s)	3510
AccBSI	CCGCTC	1 cut(s)	3417
AccI	GTMKAC	3 cut(s)	147, 393, 3759
AccII	CGCG	7 cut(s)	215, 1073, 1130, 1551, 1608, 1949, 2027
AccIII	TCCGGA	1 cut(s)	2048
AclI	AACGTT	1 cut(s)	3798
AcoI	YGGCCR	3 cut(s)	223, 917, 2314
AcsI	RAATTY	8 cut(s)	576, 692, 1133, 1776, 2566, 3072, 3132, 4442
AcuI	CTGAAG	5 cut(s)	863, 906, 1766, 2371, 3945
AcyI	GRCGYC	1 cut(s)	2382
AfeI	AGCGCT	1 cut(s)	2445
AjiI	CACGTC	1 cut(s)	4153
AjnI	CCWGG	1 cut(s)	2574
AjuI	GAANNNNNNNTTGG	2 cut(s)	3769, 3801
AleI	CACNNNNGTG	1 cut(s)	71
Alw21I	GWGCWC	5 cut(s)	133, 271, 1679, 3022, 3807
Alw26I	GTCTC	6 cut(s)	708, 2517, 2874, 2998, 4029, 4197
Alw44I	GTGCAC	1 cut(s)	1675
Ama87I	CYCGRG	2 cut(s)	3330, 3602
Aor13HI	TCCGGA	1 cut(s)	2048
Aor51HI	AGCGCT	1 cut(s)	2445
ApaLI	GTGCAC	1 cut(s)	1675
ApoI	RAATTY	8 cut(s)	576, 692, 1133, 1776, 2566, 3072, 3132, 4442
ArsI	GACNNNNNNTTYG	2 cut(s)	1702, 1734
Asp700I	GAANNNNTTC	2 cut(s)	2056, 4102
Asp718I	GGTACC	1 cut(s)	3303
AspLEI	GCGC	5 cut(s)	454, 1130, 1551, 2446, 4378
AspS9I	GGNCC	8 cut(s)	1037, 1378, 2412, 2663, 2728, 3258, 3501, 3660
AsuC2I	CCSGG	4 cut(s)	10, 872, 2216, 3308
AsuHPI	GGTGA	9 cut(s)	322, 1148, 2093, 2723, 2912, 3190, 3415, 3893, 4405
AsuII	TTCGAA	2 cut(s)	740, 952
AvaI	CYCGRG	2 cut(s)	3330, 3602
AvaII	GGWCC	4 cut(s)	2412, 2663, 2728, 3501
BaeGI	GKGCMC	1 cut(s)	1679
BaeI	ACNNNNGTAYC	4 cut(s)	820, 853, 3295, 3328
BalI	TGGCCA	2 cut(s)	919, 2316
BamHI	GGATCC	1 cut(s)	2045
BanI	GGYRCC	2 cut(s)	3303, 4487
BanII	GRGCYC	3 cut(s)	133, 271, 3807
BarI	GAAGNNNNNNTAC	2 cut(s)	1104, 1136
BauI	CACGAG	2 cut(s)	2340, 3789
BbsI	GAAGAC	3 cut(s)	1914, 2049, 2377
Bbv12I	GWGCWC	5 cut(s)	133, 271, 1679, 3022, 3807
BceAI	ACGGC	1 cut(s)	56
BcgI	CGANNNNNNTGC	4 cut(s)	1516, 1550, 4274, 4308
BciT130I	CCWGG	1 cut(s)	2576
BciVI	GTATCC	4 cut(s)	543, 1094, 2322, 3568
BclI	TGATCA	4 cut(s)	435, 1011, 1554, 3154
BcnI	CCSGG	4 cut(s)	10, 872, 2216, 3308
BcoDI	GTCTC	6 cut(s)	708, 2517, 2874, 2998, 4029, 4197
BfaI	CTAG	4 cut(s)	363, 588, 4433, 4437
BfmI	CTRYAG	2 cut(s)	1328, 2925
BfoI	RGCGCY	1 cut(s)	2447
BfuAI	ACCTGC	1 cut(s)	3808
BfuI	GTATCC	4 cut(s)	543, 1094, 2322, 3568
BglI	GCCNNNNNGGC	1 cut(s)	2135
BlpI	GCTNAGC	1 cut(s)	1249
BmcAI	AGTACT	2 cut(s)	1686, 2635
Bme1390I	CCNGG	5 cut(s)	10, 872, 2216, 2576, 3308
Bme18I	GGWCC	4 cut(s)	2412, 2663, 2728, 3501
BmeT110I	CYCGRG	2 cut(s)	3330, 3602
BmgBI	CACGTC	1 cut(s)	4153
BmgT120I	GGNCC	8 cut(s)	1037, 1378, 2412, 2663, 2728, 3258, 3501, 3660
BmiI	GGNNCC	8 cut(s)	585, 1788, 2047, 2164, 2413, 3305, 3502, 4489
BmrFI	CCNGG	5 cut(s)	10, 872, 2216, 2576, 3308
BmrI	ACTGGG	3 cut(s)	681, 1676, 4085
BmuI	ACTGGG	3 cut(s)	681, 1676, 4085
BpiI	GAAGAC	3 cut(s)	1914, 2049, 2377
BplI	GAGNNNNNCTC	2 cut(s)	258, 290
Bpu1102I	GCTNAGC	1 cut(s)	1249
Bpu14I	TTCGAA	2 cut(s)	740, 952
BpuEI	CTTGAG	3 cut(s)	555, 1812, 4029
BpuMI	CCSGG	4 cut(s)	10, 872, 2216, 3308
Bsa29I	ATCGAT	4 cut(s)	1858, 3168, 3981, 3990
BsaAI	YACGTR	2 cut(s)	2134, 3479
BsaBI	GATNNNNATC	1 cut(s)	3410
BsaHI	GRCGYC	1 cut(s)	2382
BsaI	GGTCTC	1 cut(s)	4029
BsaWI	WCCGGW	2 cut(s)	966, 2048
BsaXI	ACNNNNNCTCC	8 cut(s)	705, 735, 1157, 1187, 2594, 2624, 3349, 3379
Bse118I	RCCGGY	2 cut(s)	1354, 2786
Bse1I	ACTGG	6 cut(s)	676, 763, 947, 1682, 2912, 4091
Bse3DI	GCAATG	3 cut(s)	1978, 3201, 4371
Bse8I	GATNNNNATC	1 cut(s)	3410
BseAI	TCCGGA	1 cut(s)	2048
BseBI	CCWGG	1 cut(s)	2576
BseCI	ATCGAT	4 cut(s)	1858, 3168, 3981, 3990
BseJI	GATNNNNATC	1 cut(s)	3410
BseMI	GCAATG	3 cut(s)	1978, 3201, 4371
BseNI	ACTGG	6 cut(s)	676, 763, 947, 1682, 2912, 4091
BseRI	GAGGAG	4 cut(s)	143, 278, 2789, 3762
BseSI	GKGCMC	1 cut(s)	1679
BseX3I	CGGCCG	1 cut(s)	223
BseYI	CCCAGC	4 cut(s)	1456, 1742, 1956, 2645
BsgI	GTGCAG	5 cut(s)	1319, 1422, 1698, 2806, 2958
Bsh1236I	CGCG	7 cut(s)	215, 1073, 1130, 1551, 1608, 1949, 2027
Bsh1285I	CGRYCG	1 cut(s)	226
BshNI	GGYRCC	2 cut(s)	3303, 4487
BshVI	ATCGAT	4 cut(s)	1858, 3168, 3981, 3990
BsiEI	CGRYCG	1 cut(s)	226
BsiHKAI	GWGCWC	5 cut(s)	133, 271, 1679, 3022, 3807
BsiHKCI	CYCGRG	2 cut(s)	3330, 3602
BsiSI	CCGG	9 cut(s)	9, 872, 967, 1355, 2049, 2215, 2571, 2787, 3307
BslFI	GGGAC	8 cut(s)	25, 1723, 1759, 1973, 2050, 3622, 4233, 4257
BsmAI	GTCTC	6 cut(s)	708, 2517, 2874, 2998, 4029, 4197
BsmBI	CGTCTC	2 cut(s)	708, 2517
BsmFI	GGGAC	8 cut(s)	25, 1723, 1759, 1973, 2050, 3622, 4233, 4257
BsmI	GAATGC	3 cut(s)	348, 1799, 3432
Bso31I	GGTCTC	1 cut(s)	4029
BsoBI	CYCGRG	2 cut(s)	3330, 3602
Bsp119I	TTCGAA	2 cut(s)	740, 952
Bsp1286I	GDGCHC	5 cut(s)	133, 271, 1679, 3022, 3807
Bsp13I	TCCGGA	1 cut(s)	2048
Bsp1407I	TGTACA	3 cut(s)	1221, 1729, 4358
Bsp1720I	GCTNAGC	1 cut(s)	1249
Bsp19I	CCATGG	2 cut(s)	380, 2613
BspDI	ATCGAT	4 cut(s)	1858, 3168, 3981, 3990
BspEI	TCCGGA	1 cut(s)	2048
BspFNI	CGCG	7 cut(s)	215, 1073, 1130, 1551, 1608, 1949, 2027
BspHI	TCATGA	2 cut(s)	2517, 3570
BspLI	GGNNCC	8 cut(s)	585, 1788, 2047, 2164, 2413, 3305, 3502, 4489
BspMAI	CTGCAG	2 cut(s)	1332, 2929
BspMI	ACCTGC	1 cut(s)	3808
BspQI	GCTCTTC	1 cut(s)	4118
BspT104I	TTCGAA	2 cut(s)	740, 952
BspT107I	GGYRCC	2 cut(s)	3303, 4487
BspTNI	GGTCTC	1 cut(s)	4029
BsrBI	CCGCTC	1 cut(s)	3417
BsrDI	GCAATG	3 cut(s)	1978, 3201, 4371
BsrFI	RCCGGY	2 cut(s)	1354, 2786
BsrGI	TGTACA	3 cut(s)	1221, 1729, 4358
BsrI	ACTGG	6 cut(s)	676, 763, 947, 1682, 2912, 4091
BssAI	RCCGGY	2 cut(s)	1354, 2786
BssNI	GRCGYC	1 cut(s)	2382
BssSI	CACGAG	2 cut(s)	2340, 3789
BssT1I	CCWWGG	5 cut(s)	380, 1396, 2613, 3444, 4491
Bst2BI	CACGAG	2 cut(s)	2340, 3789
Bst2UI	CCWGG	1 cut(s)	2576
Bst6I	CTCTTC	8 cut(s)	161, 455, 498, 1971, 2692, 2766, 4107, 4118
BstACI	GRCGYC	1 cut(s)	2382
BstAPI	GCANNNNNTGC	2 cut(s)	2936, 2984
BstAUI	TGTACA	3 cut(s)	1221, 1729, 4358
BstBAI	YACGTR	2 cut(s)	2134, 3479
BstBI	TTCGAA	2 cut(s)	740, 952
BstC8I	GCNNGC	8 cut(s)	1260, 1625, 2018, 2801, 2883, 2937, 3093, 4463
BstDSI	CCRYGG	6 cut(s)	45, 213, 380, 1071, 2613, 2659
BstENI	CCTNNNNNAGG	2 cut(s)	293, 3963
BstFNI	CGCG	7 cut(s)	215, 1073, 1130, 1551, 1608, 1949, 2027
BstH2I	RGCGCY	1 cut(s)	2447
BstHHI	GCGC	5 cut(s)	454, 1130, 1551, 2446, 4378
BstMAI	GTCTC	6 cut(s)	708, 2517, 2874, 2998, 4029, 4197
BstMCI	CGRYCG	1 cut(s)	226
BstNI	CCWGG	1 cut(s)	2576
BstNSI	RCATGY	1 cut(s)	3095
BstSCI	CCNGG	5 cut(s)	8, 870, 2214, 2574, 3306
BstSFI	CTRYAG	2 cut(s)	1328, 2925
BstSLI	GKGCMC	1 cut(s)	1679
BstSNI	TACGTA	1 cut(s)	3479
BstUI	CGCG	7 cut(s)	215, 1073, 1130, 1551, 1608, 1949, 2027
BstV2I	GAAGAC	3 cut(s)	1914, 2049, 2377
BstX2I	RGATCY	1 cut(s)	2045
BstXI	CCANNNNNNTGG	4 cut(s)	1184, 1375, 1663, 3497
BstYI	RGATCY	1 cut(s)	2045
BstZI	CGGCCG	1 cut(s)	223
Bsu15I	ATCGAT	4 cut(s)	1858, 3168, 3981, 3990
BsuI	GTATCC	4 cut(s)	543, 1094, 2322, 3568
BsuTUI	ATCGAT	4 cut(s)	1858, 3168, 3981, 3990
BtgI	CCRYGG	6 cut(s)	45, 213, 380, 1071, 2613, 2659
BtgZI	GCGATG	1 cut(s)	1354
BtrI	CACGTC	1 cut(s)	4153
BtsI	GCAGTG	2 cut(s)	438, 4290
BtsIMutI	CAGTG	9 cut(s)	141, 438, 1755, 2046, 2395, 3261, 4290, 4305, 4478
BveI	ACCTGC	1 cut(s)	3808
Cac8I	GCNNGC	8 cut(s)	1260, 1625, 2018, 2801, 2883, 2937, 3093, 4463
CciI	TCATGA	2 cut(s)	2517, 3570
CciNI	GCGGCCGC	1 cut(s)	223
CfoI	GCGC	5 cut(s)	454, 1130, 1551, 2446, 4378
Cfr10I	RCCGGY	2 cut(s)	1354, 2786
Cfr13I	GGNCC	8 cut(s)	1037, 1378, 2412, 2663, 2728, 3258, 3501, 3660
Cfr42I	CCGCGG	2 cut(s)	216, 1074
ClaI	ATCGAT	4 cut(s)	1858, 3168, 3981, 3990
CseI	GACGC	2 cut(s)	725, 2931
CspCI	CAANNNNNGTGG	6 cut(s)	429, 464, 501, 536, 2290, 2325
DraI	TTTAAA	1 cut(s)	3867
DrdI	GACNNNNNNGTC	1 cut(s)	3818
DseDI	GACNNNNNNGTC	1 cut(s)	3818
EaeI	YGGCCR	3 cut(s)	223, 917, 2314
EagI	CGGCCG	1 cut(s)	223
Eam1104I	CTCTTC	8 cut(s)	161, 455, 498, 1971, 2692, 2766, 4107, 4118
EarI	CTCTTC	8 cut(s)	161, 455, 498, 1971, 2692, 2766, 4107, 4118
EciI	GGCGGA	1 cut(s)	1483
Ecl136II	GAGCTC	3 cut(s)	131, 269, 3805
EclXI	CGGCCG	1 cut(s)	223
Eco105I	TACGTA	1 cut(s)	3479
Eco130I	CCWWGG	5 cut(s)	380, 1396, 2613, 3444, 4491
Eco24I	GRGCYC	3 cut(s)	133, 271, 3807
Eco31I	GGTCTC	1 cut(s)	4029
Eco32I	GATATC	2 cut(s)	1818, 3827
Eco47I	GGWCC	4 cut(s)	2412, 2663, 2728, 3501
Eco47III	AGCGCT	1 cut(s)	2445
Eco52I	CGGCCG	1 cut(s)	223
Eco53kI	GAGCTC	3 cut(s)	131, 269, 3805
Eco57I	CTGAAG	5 cut(s)	863, 906, 1766, 2371, 3945
Eco88I	CYCGRG	2 cut(s)	3330, 3602
EcoICRI	GAGCTC	3 cut(s)	131, 269, 3805
EcoNI	CCTNNNNNAGG	2 cut(s)	293, 3963
EcoRI	GAATTC	2 cut(s)	576, 3132
EcoRII	CCWGG	1 cut(s)	2574
EcoRV	GATATC	2 cut(s)	1818, 3827
EcoT14I	CCWWGG	5 cut(s)	380, 1396, 2613, 3444, 4491
EcoT38I	GRGCYC	3 cut(s)	133, 271, 3807
ErhI	CCWWGG	5 cut(s)	380, 1396, 2613, 3444, 4491
Esp3I	CGTCTC	2 cut(s)	708, 2517
FaqI	GGGAC	8 cut(s)	25, 1723, 1759, 1973, 2050, 3622, 4233, 4257
FauI	CCCGC	6 cut(s)	209, 492, 1066, 1812, 2111, 4466
FauNDI	CATATG	1 cut(s)	3242
FbaI	TGATCA	4 cut(s)	435, 1011, 1554, 3154
FblI	GTMKAC	3 cut(s)	147, 393, 3759
FriOI	GRGCYC	3 cut(s)	133, 271, 3807
FspBI	CTAG	4 cut(s)	363, 588, 4433, 4437
FspI	TGCGCA	1 cut(s)	4377
GlaI	GCGC	5 cut(s)	453, 1129, 1550, 2445, 4377
GsaI	CCCAGC	4 cut(s)	1460, 1746, 1960, 2649
HaeII	RGCGCY	1 cut(s)	2447
HapII	CCGG	9 cut(s)	9, 872, 967, 1355, 2049, 2215, 2571, 2787, 3307
HgaI	GACGC	2 cut(s)	725, 2931
HhaI	GCGC	5 cut(s)	454, 1130, 1551, 2446, 4378
Hin1I	GRCGYC	1 cut(s)	2382
Hin6I	GCGC	5 cut(s)	452, 1128, 1549, 2444, 4376
HinP1I	GCGC	5 cut(s)	452, 1128, 1549, 2444, 4376
HincII	GTYRAC	5 cut(s)	148, 324, 850, 2008, 3339
HindII	GTYRAC	5 cut(s)	148, 324, 850, 2008, 3339
HindIII	AAGCTT	6 cut(s)	191, 1181, 1566, 1599, 2797, 3433
HpaI	GTTAAC	2 cut(s)	2008, 3339
HpaII	CCGG	9 cut(s)	9, 872, 967, 1355, 2049, 2215, 2571, 2787, 3307
HphI	GGTGA	9 cut(s)	322, 1148, 2093, 2723, 2912, 3190, 3415, 3893, 4405
HpyCH4IV	ACGT	6 cut(s)	2133, 2382, 2695, 3478, 3798, 4152
HpySE526I	ACGT	6 cut(s)	2133, 2382, 2695, 3478, 3798, 4152
Hsp92I	GRCGYC	1 cut(s)	2382
HspAI	GCGC	5 cut(s)	452, 1128, 1549, 2444, 4376
Kpn2I	TCCGGA	1 cut(s)	2048
KpnI	GGTACC	1 cut(s)	3307
Ksp22I	TGATCA	4 cut(s)	435, 1011, 1554, 3154
KspAI	GTTAAC	2 cut(s)	2008, 3339
KspI	CCGCGG	2 cut(s)	216, 1074
LguI	GCTCTTC	1 cut(s)	4118
LmnI	GCTCC	6 cut(s)	583, 1460, 1786, 2441, 3370, 4212
MaeI	CTAG	4 cut(s)	363, 588, 4433, 4437
MaeII	ACGT	6 cut(s)	2133, 2382, 2695, 3478, 3798, 4152
MaeIII	GTNAC	8 cut(s)	73, 703, 754, 2360, 2470, 3196, 3311, 4476
MbiI	CCGCTC	1 cut(s)	3417
MfeI	CAATTG	1 cut(s)	4207
MflI	RGATCY	1 cut(s)	2045
MhlI	GDGCHC	5 cut(s)	133, 271, 1679, 3022, 3807
MlsI	TGGCCA	2 cut(s)	919, 2316
MluNI	TGGCCA	2 cut(s)	919, 2316
MlyI	GAGTC	4 cut(s)	1437, 1604, 4257, 4263
MmeI	TCCRAC	4 cut(s)	258, 1252, 1833, 4075
Mox20I	TGGCCA	2 cut(s)	919, 2316
MroI	TCCGGA	1 cut(s)	2048
MroXI	GAANNNNTTC	2 cut(s)	2056, 4102
MscI	TGGCCA	2 cut(s)	919, 2316
MslI	CAYNNNNRTG	4 cut(s)	31, 71, 3206, 3495
Msp20I	TGGCCA	2 cut(s)	919, 2316
MspA1I	CMGCKG	5 cut(s)	215, 841, 1073, 1333, 1418
MspI	CCGG	9 cut(s)	9, 872, 967, 1355, 2049, 2215, 2571, 2787, 3307
MspR9I	CCNGG	5 cut(s)	10, 872, 2216, 2576, 3308
MssI	GTTTAAAC	1 cut(s)	3867
MunI	CAATTG	1 cut(s)	4207
Mva1269I	GAATGC	3 cut(s)	348, 1799, 3432
MvaI	CCWGG	1 cut(s)	2576
MvnI	CGCG	7 cut(s)	215, 1073, 1130, 1551, 1608, 1949, 2027
NciI	CCSGG	4 cut(s)	10, 872, 2216, 3308
NcoI	CCATGG	2 cut(s)	380, 2613
NdeI	CATATG	1 cut(s)	3242
NlaIV	GGNNCC	8 cut(s)	585, 1788, 2047, 2164, 2413, 3305, 3502, 4489
NmeAIII	GCCGAG	2 cut(s)	1234, 3609
NmuCI	GTSAC	5 cut(s)	73, 754, 2360, 3196, 4476
NotI	GCGGCCGC	1 cut(s)	223
NsbI	TGCGCA	1 cut(s)	4377
NspI	RCATGY	1 cut(s)	3095
NspV	TTCGAA	2 cut(s)	740, 952
OliI	CACNNNNGTG	1 cut(s)	71
PaeI	GCATGC	1 cut(s)	3095
PaeR7I	CTCGAG	1 cut(s)	3330
PagI	TCATGA	2 cut(s)	2517, 3570
PciSI	GCTCTTC	1 cut(s)	4118
PcsI	WCGNNNNNNNCGW	1 cut(s)	3987
PctI	GAATGC	3 cut(s)	348, 1799, 3432
PdmI	GAANNNNTTC	2 cut(s)	2056, 4102
PflMI	CCANNNNNTGG	1 cut(s)	3510
PfoI	TCCNGGA	1 cut(s)	8
PleI	GAGTC	4 cut(s)	1436, 1604, 4256, 4263
PmeI	GTTTAAAC	1 cut(s)	3867
PpsI	GAGTC	4 cut(s)	1436, 1604, 4256, 4263
Ppu21I	YACGTR	2 cut(s)	2134, 3479
PsiI	TTATAA	2 cut(s)	2966, 3596
Psp124BI	GAGCTC	3 cut(s)	133, 271, 3807
Psp1406I	AACGTT	1 cut(s)	3798
Psp6I	CCWGG	1 cut(s)	2574
PspFI	CCCAGC	4 cut(s)	1456, 1742, 1956, 2645
PspGI	CCWGG	1 cut(s)	2574
PspN4I	GGNNCC	8 cut(s)	585, 1788, 2047, 2164, 2413, 3305, 3502, 4489
PspPI	GGNCC	8 cut(s)	1037, 1378, 2412, 2663, 2728, 3258, 3501, 3660
PsrI	GAACNNNNNNTAC	4 cut(s)	882, 914, 3750, 3782
PstI	CTGCAG	2 cut(s)	1332, 2929
PsuI	RGATCY	1 cut(s)	2045
PvuII	CAGCTG	2 cut(s)	1333, 1418
RseI	CAYNNNNRTG	4 cut(s)	31, 71, 3206, 3495
SacI	GAGCTC	3 cut(s)	133, 271, 3807
SacII	CCGCGG	2 cut(s)	216, 1074
SalI	GTCGAC	1 cut(s)	146
SapI	GCTCTTC	1 cut(s)	4118
Sau96I	GGNCC	8 cut(s)	1037, 1378, 2412, 2663, 2728, 3258, 3501, 3660
ScaI	AGTACT	2 cut(s)	1686, 2635
SchI	GAGTC	4 cut(s)	1437, 1604, 4257, 4263
ScrFI	CCNGG	5 cut(s)	10, 872, 2216, 2576, 3308
SduI	GDGCHC	5 cut(s)	133, 271, 1679, 3022, 3807
SfcI	CTRYAG	2 cut(s)	1328, 2925
Sfr274I	CTCGAG	1 cut(s)	3330
Sfr303I	CCGCGG	2 cut(s)	216, 1074
SfuI	TTCGAA	2 cut(s)	740, 952
SgrBI	CCGCGG	2 cut(s)	216, 1074
SinI	GGWCC	4 cut(s)	2412, 2663, 2728, 3501
SlaI	CTCGAG	1 cut(s)	3330
SmiMI	CAYNNNNRTG	4 cut(s)	31, 71, 3206, 3495
SmlI	CTYRAG	4 cut(s)	570, 1827, 3330, 4044
SmoI	CTYRAG	4 cut(s)	570, 1827, 3330, 4044
SnaBI	TACGTA	1 cut(s)	3479
SphI	GCATGC	1 cut(s)	3095
SspI	AATATT	1 cut(s)	2421
SspMI	CTAG	4 cut(s)	363, 588, 4433, 4437
SstI	GAGCTC	3 cut(s)	133, 271, 3807
StyD4I	CCNGG	5 cut(s)	8, 870, 2214, 2574, 3306
StyI	CCWWGG	5 cut(s)	380, 1396, 2613, 3444, 4491
TaiI	ACGT	6 cut(s)	2136, 2385, 2698, 3481, 3801, 4155
TaqII	GACCGA	1 cut(s)	4328
TatI	WGTACW	7 cut(s)	1221, 1684, 1729, 2536, 2633, 3210, 4358
TauI	GCSGC	3 cut(s)	225, 228, 1949
TscAI	CASTG	9 cut(s)	148, 438, 1755, 2046, 2395, 3268, 4297, 4305, 4485
TseFI	GTSAC	5 cut(s)	73, 754, 2360, 3196, 4476
Tsp45I	GTSAC	5 cut(s)	73, 754, 2360, 3196, 4476
TspGWI	ACGGA	8 cut(s)	34, 726, 2175, 2676, 2840, 2850, 3645, 4215
TspRI	CASTG	9 cut(s)	148, 438, 1755, 2046, 2395, 3268, 4297, 4305, 4485
Van91I	CCANNNNNTGG	1 cut(s)	3510
VneI	GTGCAC	1 cut(s)	1675
VpaK11BI	GGWCC	4 cut(s)	2412, 2663, 2728, 3501
XagI	CCTNNNNNAGG	2 cut(s)	293, 3963
XapI	RAATTY	8 cut(s)	576, 692, 1133, 1776, 2566, 3072, 3132, 4442
XceI	RCATGY	1 cut(s)	3095
XcmI	CCANNNNNNNNNTGG	4 cut(s)	87, 3478, 3511, 4297
XhoI	CTCGAG	1 cut(s)	3330
XmiI	GTMKAC	3 cut(s)	147, 393, 3759
XmnI	GAANNNNTTC	2 cut(s)	2056, 4102
XspI	CTAG	4 cut(s)	363, 588, 4433, 4437
ZraI	GACGTC	1 cut(s)	2383
ZrmI	AGTACT	2 cut(s)	1686, 2635

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.