FvH4_2g25920

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007242
Physical Location & Seq
Forward (+)
20903878 .. 20905891
2014 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25920.t1

Sequence Viewer

Length: 564 bp
ATGGGTATCGATTTGGTCGCCGGCGGCAAGTCAAAGAACACCAGGCGCACATCGCCTAGGTCTCAGGATATCTACCTCAAGCTCACGGTCAAGCTTTATGACTTCATTCACCGAAGGACCAAGAGCCATTTCAGCAAGGTGATTCGTCACAGGCTTTGCATGAGCAAGGTCAACAAGGCTCCTCTCTCGCTGTCCCGACTGTGTCGGTATATGCAGGGCCATGAGGACAAGATTGCTGTGCTCGTCGGGACTGTCACCGACGATGTTCGCATCGCTGAGGTCCCAGCTCTCAAGGTCACTGCTCTCAGGTTCACCGAGAGAGCTAGGGCTAGGATTGAGAAGGCTGGTGGAGAGTGCTTGACTTTTGACCAGCTGGCTCTCAGAGCTCCGCTTGGGCAGAACACGATGCTGTTACGAGGACCCAAGAATGCTCGTGAAGCTGTGAAGCATTTCGGTCCAGCACCTGGTGTCCCGCACAGCCACACCAAGCCATATGTGCGATCAAAGGGCAGGAAGTTCGAGAAGGCCAGGGGAAAAAGAAACAGCCGTGGTTTCCGTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18

Protein Analysis

188

Amino Acids

21.1

Weight (kDa)

11.11

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18 PF17135 2 - 187 5.3e-89 Ribosomal protein 60S L18 and 50S L18e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 464
AciI CCGC 3 cut(s) 24, 389, 473
AdeI CACNNNGTG 1 cut(s) 467
AfiI CCNNNNNNNGG 1 cut(s) 464
AjnI CCWGG 3 cut(s) 41, 463, 527
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 7 cut(s) 82, 94, 287, 323, 373, 386, 440
AluI AGCT 7 cut(s) 82, 94, 287, 323, 373, 386, 440
Alw21I GWGCWC 2 cut(s) 243, 388
Alw26I GTCTC 1 cut(s) 66
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 2 cut(s) 217, 525
Asp700I GAANNNNTTC 1 cut(s) 449
AspA2I CCTAGG 1 cut(s) 56
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 5 cut(s) 117, 217, 280, 419, 455
AsuHPI GGTGA 4 cut(s) 101, 151, 247, 304
AvaII GGWCC 4 cut(s) 117, 280, 419, 455
AvrII CCTAGG 1 cut(s) 56
BanII GRGCYC 1 cut(s) 388
BauI CACGAG 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 243, 388
BbvCI CCTCAGC 1 cut(s) 276
BceAI ACGGC 1 cut(s) 531
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 3 cut(s) 43, 465, 529
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 3 cut(s) 57, 324, 330
BisI GCNGC 1 cut(s) 25
BlnI CCTAGG 1 cut(s) 56
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 3 cut(s) 43, 465, 529
Bme18I GGWCC 4 cut(s) 117, 280, 419, 455
BmgT120I GGNCC 5 cut(s) 117, 217, 280, 419, 455
BmiI GGNNCC 3 cut(s) 180, 282, 421
BmrFI CCNGG 3 cut(s) 43, 465, 529
BmsI GCATC 2 cut(s) 279, 396
Bpu10I CCTNAGC 1 cut(s) 276
BpuEI CTTGAG 2 cut(s) 62, 275
Bsa29I ATCGAT 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 56, 528, 547
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 1 cut(s) 20
BseBI CCWGG 3 cut(s) 43, 465, 529
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 3 cut(s) 56, 528, 547
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMII CTCAG 4 cut(s) 77, 267, 319, 394
BseRI GAGGAG 1 cut(s) 171
BseYI CCCAGC 1 cut(s) 283
BshFI GGCC 2 cut(s) 219, 527
BshVI ATCGAT 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 243, 388
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 4 cut(s) 178, 262, 266, 455
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 66
BsmFI GGGAC 4 cut(s) 178, 262, 266, 455
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 2 cut(s) 219, 527
Bso31I GGTCTC 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 243, 388
Bsp143I GATC 1 cut(s) 500
BspACI CCGC 3 cut(s) 24, 389, 473
BspANI GGCC 2 cut(s) 219, 527
BspCNI CTCAG 4 cut(s) 76, 268, 318, 393
BspDI ATCGAT 1 cut(s) 9
BspLI GGNNCC 3 cut(s) 180, 282, 421
BspTNI GGTCTC 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 3 cut(s) 56, 528, 547
BssMI GATC 1 cut(s) 500
BssSI CACGAG 1 cut(s) 432
BssT1I CCWWGG 1 cut(s) 56
Bst2BI CACGAG 1 cut(s) 432
Bst2UI CCWGG 3 cut(s) 43, 465, 529
Bst4CI ACNGT 3 cut(s) 88, 201, 253
BstC8I GCNNGC 2 cut(s) 22, 375
BstDEI CTNAG 4 cut(s) 63, 276, 305, 380
BstDSI CCRYGG 1 cut(s) 547
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 1 cut(s) 503
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 1 cut(s) 500
BstMWI GCNNNNNNNGC 5 cut(s) 52, 132, 383, 437, 496
BstNI CCWGG 3 cut(s) 43, 465, 529
BstSCI CCNGG 3 cut(s) 41, 463, 527
Bsu15I ATCGAT 1 cut(s) 9
BsuRI GGCC 2 cut(s) 219, 527
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 547
BtgZI GCGATG 2 cut(s) 36, 256
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 2 cut(s) 22, 375
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 5 cut(s) 117, 217, 280, 419, 455
ClaI ATCGAT 1 cut(s) 9
CsiI ACCWGGT 1 cut(s) 463
CviAII CATG 2 cut(s) 160, 221
DdeI CTNAG 4 cut(s) 63, 276, 305, 380
DpnI GATC 1 cut(s) 502
DpnII GATC 1 cut(s) 500
DraIII CACNNNGTG 1 cut(s) 467
Ecl136II GAGCTC 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 70
Eco47I GGWCC 4 cut(s) 117, 280, 419, 455
Eco53kI GAGCTC 1 cut(s) 386
EcoICRI GAGCTC 1 cut(s) 386
EcoO109I RGGNCCY 2 cut(s) 280, 419
EcoRII CCWGG 3 cut(s) 41, 463, 527
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 56
EcoT38I GRGCYC 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 2 cut(s) 163, 224
FaiI YATR 7 cut(s) 99, 161, 210, 212, 222, 493, 495
FaqI GGGAC 4 cut(s) 178, 262, 266, 455
FatI CATG 2 cut(s) 159, 220
FauI CCCGC 1 cut(s) 480
FauNDI CATATG 1 cut(s) 493
Fnu4HI GCNGC 1 cut(s) 25
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 1 cut(s) 25
FspBI CTAG 3 cut(s) 57, 324, 330
GlaI GCGC 1 cut(s) 47
GluI GCNGC 1 cut(s) 25
GsaI CCCAGC 1 cut(s) 287
HaeIII GGCC 2 cut(s) 219, 527
HapII CCGG 1 cut(s) 21
HhaI GCGC 1 cut(s) 48
Hin1II CATG 2 cut(s) 163, 224
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 1 cut(s) 142
HpaII CCGG 1 cut(s) 21
HphI GGTGA 4 cut(s) 101, 151, 247, 304
Hpy166II GTNNAC 2 cut(s) 172, 312
Hpy188I TCNGA 1 cut(s) 383
Hpy188III TCNNGA 5 cut(s) 65, 195, 247, 434, 520
Hpy8I GTNNAC 2 cut(s) 172, 312
Hpy99I CGWCG 2 cut(s) 248, 263
HpyAV CCTTC 3 cut(s) 108, 334, 517
HpyCH4III ACNGT 3 cut(s) 88, 201, 253
HpyCH4V TGCA 2 cut(s) 159, 214
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 132, 383, 437, 496
HpyF3I CTNAG 4 cut(s) 63, 276, 305, 380
Hsp92II CATG 2 cut(s) 163, 224
HspAI GCGC 1 cut(s) 46
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 1 cut(s) 500
LmnI GCTCC 2 cut(s) 184, 391
LweI GCATC 2 cut(s) 279, 396
MabI ACCWGGT 1 cut(s) 463
MaeI CTAG 3 cut(s) 57, 324, 330
MaeIII GTNAC 4 cut(s) 146, 253, 295, 411
MalI GATC 1 cut(s) 502
MboI GATC 1 cut(s) 500
MhlI GDGCHC 2 cut(s) 243, 388
MnlI CCTC 5 cut(s) 86, 192, 217, 271, 410
MreI CGCCGGCG 1 cut(s) 20
MroNI GCCGGC 1 cut(s) 20
MroXI GAANNNNTTC 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 3 cut(s) 43, 465, 529
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 3 cut(s) 43, 465, 529
MwoI GCNNNNNNNGC 5 cut(s) 52, 132, 383, 437, 496
NaeI GCCGGC 1 cut(s) 22
NdeI CATATG 1 cut(s) 493
NdeII GATC 1 cut(s) 500
NgoMIV GCCGGC 1 cut(s) 20
NlaIII CATG 2 cut(s) 163, 224
NlaIV GGNNCC 3 cut(s) 180, 282, 421
NmuCI GTSAC 3 cut(s) 146, 253, 295
PctI GAATGC 1 cut(s) 433
PdiI GCCGGC 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 142
PflFI GACNNNGTC 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 464
PkrI GCNGC 1 cut(s) 26
PpuMI RGGWCCY 2 cut(s) 280, 419
Psp124BI GAGCTC 1 cut(s) 388
Psp5II RGGWCCY 2 cut(s) 280, 419
Psp6I CCWGG 3 cut(s) 41, 463, 527
PspFI CCCAGC 1 cut(s) 283
PspGI CCWGG 3 cut(s) 41, 463, 527
PspN4I GGNNCC 3 cut(s) 180, 282, 421
PspPI GGNCC 5 cut(s) 117, 217, 280, 419, 455
PspPPI RGGWCCY 2 cut(s) 280, 419
PstNI CAGNNNCTG 1 cut(s) 464
PsyI GACNNNGTC 1 cut(s) 201
PvuII CAGCTG 1 cut(s) 373
SacI GAGCTC 1 cut(s) 388
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 1 cut(s) 500
Sau96I GGNCC 5 cut(s) 117, 217, 280, 419, 455
ScrFI CCNGG 3 cut(s) 43, 465, 529
SduI GDGCHC 2 cut(s) 243, 388
SexAI ACCWGGT 1 cut(s) 463
SfaNI GCATC 2 cut(s) 279, 396
SgrAI CRCCGGYG 1 cut(s) 20
SinI GGWCC 4 cut(s) 117, 280, 419, 455
SmlI CTYRAG 2 cut(s) 77, 290
SmoI CTYRAG 2 cut(s) 77, 290
SsiI CCGC 3 cut(s) 24, 389, 473
SspMI CTAG 3 cut(s) 57, 324, 330
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 3 cut(s) 41, 463, 527
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 3 cut(s) 88, 201, 253
TaqI TCGA 2 cut(s) 9, 519
TaqII GACCGA 1 cut(s) 443
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 142
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 3 cut(s) 146, 253, 295
Tsp45I GTSAC 3 cut(s) 146, 253, 295
TspDTI ATGAA 1 cut(s) 94
TspGWI ACGGA 1 cut(s) 545
TspRI CASTG 1 cut(s) 304
Tth111I GACNNNGTC 1 cut(s) 201
Van91I CCANNNNNTGG 1 cut(s) 464
VpaK11BI GGWCC 4 cut(s) 117, 280, 419, 455
XmaJI CCTAGG 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 3 cut(s) 57, 324, 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.