Rorug06G0242800

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007242
Physical Location & Seq
Reverse (-)
38645197 .. 38648424
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0242800.1

Sequence Viewer

Length: 1170 bp
ATGGCAATGGCTTGGAGGCTTCCAACCCAGCTGGCAACCAAAACCCACCATCACCACGACCACCCCAAACTCTCATGGTGCCGCACTTTATCACCAGACCATCTCCAGCTCACCTCAATCTCTACCGCCACTGGGAGTTGTACTCGGGGTTTGGTGAGGTGTACGGCCCGGTCGGGAGTTGGGGCTGTGAATTTGGCTCCGGGGACACCTGTCAGGCCCACGAGTATACTAGTGGTTGGCGCGACTGGAACGCTGGGGAGGCAAATTGTGAGGCGAGCATTGGATGAAGGCTATGATGTTAGGTGCTTGGTTCGGCCTAGACCGGCTCCGGCGGACTTTCTCAGAGATTGGGGTGCTACTGTTGTCAATGCAGACCTTAGCAAACCGGAGACCATACCTGCAACATTGGTTGGTGTTCATACAGTTATTGATTGTGCCACGGGCCGTCCAGAGGAGCCTATCAAAAAGGTAGATTGGGAAGGGAAAGTTGCTCTAATACAATGTGCAAAGGCAATGGGGATCCAAAAATTTGTGTTCTTTTCAATCCACAACTGTGACAAGCATCCTGAAGTTCCTCTTATGGAGATCAAGTATTGCACTGAAAAGTTTCTTAAGGATTCAGGCATAAATCACATCATAATTCGTGCATGTGGATTCATGCAAGGGATTATTGGGCAGTATGCAGTACCTATATTGGAAGAGAAATCAGTTTGGGGAACAGATGCCCCTACAAGAATTGCATACATGGACACCCAGGATATAGCTCGGTTGACATTTATAGCTTTACGAAATGAGAATGTTAATGGGAAGCTTCTCACATTTGCTGGCCCTCGTGCATGGACAACCCAAGAGGTAATAACATTGTGTGAAAGGTTTGCGGGGCAAGAGGCAAACATTACCACAGTTCCTGTCTCTGTCTTAAGAGTAACCCGCCAGCTGACTAGGCTTTTTGAGTGGACAAATGATGTTGCTGATAGATTGGCATTTTCAGAGGTCCTTTCAAGTGATACTGTTTTCTCTGTTCCAATGAACGAGACATTTAGTCTTCTTGGTGTGGACGCAAAAGATATAGTTACTCTTGAAAAATATTTGCAGGATTACTTCACCAACATATTGAAGAAGTTGAAGGATCTCAAAGCACAATCTAAGCAATCTGACATTTACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18

Protein Analysis

389

Amino Acids

43.24

Weight (kDa)

8.62

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 75 - 324 3.1e-28 NmrA-like family
Epimerase PF01370 76 - 181 5.2e-10 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 80 - 229 2.9e-17 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 406
AccB1I GGYRCC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 226
AccII CGCG 1 cut(s) 242
AciI CCGC 5 cut(s) 82, 126, 332, 878, 931
AclWI GGATC 3 cut(s) 514, 527, 1137
AcsI RAATTY 2 cut(s) 190, 527
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 3 cut(s) 142, 163, 687
AfiI CCNNNNNNNGG 2 cut(s) 132, 451
AflII CTTAAG 2 cut(s) 611, 919
AgsI TTSAA 5 cut(s) 543, 1002, 1082, 1117, 1126
AhdI GACNNNNNGTC 1 cut(s) 1041
AhlI ACTAGT 1 cut(s) 229
AjnI CCWGG 1 cut(s) 753
AleI CACNNNNGTG 1 cut(s) 552
AluBI AGCT 6 cut(s) 31, 109, 764, 782, 811, 937
AluI AGCT 6 cut(s) 31, 109, 764, 782, 811, 937
Alw26I GTCTC 3 cut(s) 383, 916, 1028
AlwI GGATC 3 cut(s) 514, 527, 1137
AlwNI CAGNNNCTG 1 cut(s) 908
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 5 cut(s) 165, 215, 314, 442, 826
ApoI RAATTY 2 cut(s) 190, 527
Asp700I GAANNNNTTC 1 cut(s) 606
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 5 cut(s) 166, 216, 442, 827, 994
AsuC2I CCSGG 2 cut(s) 169, 201
AsuHPI GGTGA 5 cut(s) 44, 84, 103, 166, 1096
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 1 cut(s) 994
BamHI GGATCC 1 cut(s) 519
BanI GGYRCC 1 cut(s) 78
BauI CACGAG 2 cut(s) 220, 831
BbsI GAAGAC 1 cut(s) 1037
BccI CCATC 2 cut(s) 57, 108
BceAI ACGGC 2 cut(s) 180, 429
BciT130I CCWGG 1 cut(s) 755
BcnI CCSGG 2 cut(s) 169, 201
BcoDI GTCTC 3 cut(s) 383, 916, 1028
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 3 cut(s) 230, 318, 942
BfrI CTTAAG 2 cut(s) 611, 919
BfuAI ACCTGC 1 cut(s) 406
BisI GCNGC 1 cut(s) 82
BlsI GCNGC 1 cut(s) 83
Bme1390I CCNGG 3 cut(s) 169, 201, 755
Bme18I GGWCC 1 cut(s) 994
BmeRI GACNNNNNGTC 1 cut(s) 1041
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 5 cut(s) 166, 216, 442, 827, 994
BmiI GGNNCC 5 cut(s) 80, 198, 327, 456, 521
BmrFI CCNGG 3 cut(s) 169, 201, 755
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 2 cut(s) 571, 712
BmuI ACTGGG 1 cut(s) 141
BoxI GACNNNNGTC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 1037
BplI GAGNNNNNCTC 2 cut(s) 127, 159
BpmI CTGGAG 1 cut(s) 89
Bpu10I CCTNAGC 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 169, 201
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 200, 438, 753
BsaWI WCCGGW 1 cut(s) 385
BsaXI ACNNNNNCTCC 2 cut(s) 575, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 451
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 2 cut(s) 136, 250
Bse3DI GCAATG 2 cut(s) 12, 519
BseBI CCWGG 1 cut(s) 755
BseDI CCNNGG 3 cut(s) 200, 438, 753
BseGI GGATG 2 cut(s) 289, 562
BseLI CCNNNNNNNGG 2 cut(s) 132, 451
BseMI GCAATG 2 cut(s) 12, 519
BseMII CTCAG 1 cut(s) 355
BseNI ACTGG 2 cut(s) 136, 250
BseRI GAGGAG 1 cut(s) 467
BseYI CCCAGC 2 cut(s) 27, 253
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 1 cut(s) 173
BshFI GGCC 5 cut(s) 167, 217, 316, 444, 828
BshNI GGYRCC 1 cut(s) 78
BsiEI CGRYCG 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 5 cut(s) 169, 200, 323, 329, 386
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 2 cut(s) 132, 451
BsmAI GTCTC 3 cut(s) 383, 916, 1028
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 5 cut(s) 167, 217, 316, 444, 828
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 144
Bsp143I GATC 3 cut(s) 519, 585, 1129
BspACI CCGC 5 cut(s) 82, 126, 332, 878, 931
BspANI GGCC 5 cut(s) 167, 217, 316, 444, 828
BspCNI CTCAG 1 cut(s) 354
BspFNI CGCG 1 cut(s) 242
BspLI GGNNCC 5 cut(s) 80, 198, 327, 456, 521
BspMI ACCTGC 1 cut(s) 406
BspPI GGATC 3 cut(s) 514, 527, 1137
BspT107I GGYRCC 1 cut(s) 78
BspTI CTTAAG 2 cut(s) 611, 919
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 12, 519
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 2 cut(s) 136, 250
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 3 cut(s) 200, 438, 753
BssMI GATC 3 cut(s) 519, 585, 1129
BssNAI GTATAC 1 cut(s) 227
BssSI CACGAG 2 cut(s) 220, 831
Bst1107I GTATAC 1 cut(s) 227
Bst2BI CACGAG 2 cut(s) 220, 831
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 5 cut(s) 361, 424, 554, 904, 1012
Bst6I CTCTTC 1 cut(s) 693
BstAFI CTTAAG 2 cut(s) 611, 919
BstC8I GCNNGC 4 cut(s) 33, 276, 826, 935
BstDEI CTNAG 3 cut(s) 341, 377, 1146
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 2 cut(s) 289, 562
BstFNI CGCG 1 cut(s) 242
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 3 cut(s) 522, 588, 1132
BstMAI GTCTC 3 cut(s) 383, 916, 1028
BstMBI GATC 3 cut(s) 519, 585, 1129
BstMCI CGRYCG 1 cut(s) 173
BstMWI GCNNNNNNNGC 2 cut(s) 259, 943
BstNI CCWGG 1 cut(s) 755
BstNSI RCATGY 1 cut(s) 651
BstPAI GACNNNNGTC 1 cut(s) 209
BstSCI CCNGG 3 cut(s) 167, 199, 753
BstUI CGCG 1 cut(s) 242
BstV2I GAAGAC 1 cut(s) 1037
BstX2I RGATCY 2 cut(s) 519, 1129
BstYI RGATCY 2 cut(s) 519, 1129
BstZ17I GTATAC 1 cut(s) 227
BsuRI GGCC 5 cut(s) 167, 217, 316, 444, 828
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 2 cut(s) 289, 562
BtsIMutI CAGTG 2 cut(s) 129, 597
BveI ACCTGC 1 cut(s) 406
Cac8I GCNNGC 4 cut(s) 33, 276, 826, 935
CaiI CAGNNNCTG 1 cut(s) 908
CfoI GCGC 1 cut(s) 242
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 5 cut(s) 166, 216, 442, 827, 994
CseI GACGC 1 cut(s) 1067
Csp6I GTAC 3 cut(s) 141, 162, 686
CviAII CATG 5 cut(s) 75, 648, 658, 745, 837
CviQI GTAC 3 cut(s) 141, 162, 686
DdeI CTNAG 3 cut(s) 341, 377, 1146
DpnI GATC 3 cut(s) 521, 587, 1131
DpnII GATC 3 cut(s) 519, 585, 1129
DriI GACNNNNNGTC 1 cut(s) 1041
Eam1104I CTCTTC 1 cut(s) 693
Eam1105I GACNNNNNGTC 1 cut(s) 1041
EarI CTCTTC 1 cut(s) 693
EciI GGCGGA 1 cut(s) 347
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 994
Eco57I CTGAAG 1 cut(s) 588
Eco88I CYCGRG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 994
EcoRII CCWGG 1 cut(s) 753
FaeI CATG 5 cut(s) 78, 651, 661, 748, 840
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FaqI GGGAC 1 cut(s) 217
FatI CATG 5 cut(s) 74, 647, 657, 744, 836
FauI CCCGC 2 cut(s) 871, 938
FblI GTMKAC 1 cut(s) 226
Fnu4HI GCNGC 1 cut(s) 82
FokI GGATG 2 cut(s) 296, 549
Fsp4HI GCNGC 1 cut(s) 82
FspBI CTAG 3 cut(s) 230, 318, 942
GlaI GCGC 1 cut(s) 241
GluI GCNGC 1 cut(s) 82
GsaI CCCAGC 2 cut(s) 31, 257
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 5 cut(s) 167, 217, 316, 444, 828
HapII CCGG 5 cut(s) 169, 200, 323, 329, 386
HgaI GACGC 1 cut(s) 1067
HhaI GCGC 1 cut(s) 242
Hin1II CATG 5 cut(s) 78, 651, 661, 748, 840
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 1 cut(s) 771
HindII GTYRAC 1 cut(s) 771
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 2 cut(s) 617, 654
HpaII CCGG 5 cut(s) 169, 200, 323, 329, 386
HphI GGTGA 5 cut(s) 44, 84, 103, 166, 1096
Hpy166II GTNNAC 5 cut(s) 162, 227, 771, 957, 1057
Hpy188I TCNGA 3 cut(s) 344, 991, 1156
Hpy188III TCNNGA 4 cut(s) 174, 449, 566, 1079
Hpy8I GTNNAC 5 cut(s) 162, 227, 771, 957, 1057
HpyAV CCTTC 3 cut(s) 281, 473, 1120
HpyCH4III ACNGT 5 cut(s) 361, 424, 554, 904, 1012
HpyF10VI GCNNNNNNNGC 2 cut(s) 259, 943
HpyF3I CTNAG 3 cut(s) 341, 377, 1146
Hsp92II CATG 5 cut(s) 78, 651, 661, 748, 840
HspAI GCGC 1 cut(s) 240
Kzo9I GATC 3 cut(s) 519, 585, 1129
LmnI GCTCC 3 cut(s) 202, 331, 454
LweI GCATC 2 cut(s) 571, 712
MaeI CTAG 3 cut(s) 230, 318, 942
MaeIII GTNAC 3 cut(s) 554, 925, 1072
MalI GATC 3 cut(s) 521, 587, 1131
MboI GATC 3 cut(s) 519, 585, 1129
MboII GAAGA 3 cut(s) 710, 1037, 1129
MflI RGATCY 2 cut(s) 519, 1129
MluCI AATT 5 cut(s) 190, 264, 527, 639, 735
MmeI TCCRAC 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 3 cut(s) 612, 801, 920
MslI CAYNNNNRTG 1 cut(s) 552
MspA1I CMGCKG 2 cut(s) 31, 937
MspCI CTTAAG 2 cut(s) 611, 919
MspI CCGG 5 cut(s) 169, 200, 323, 329, 386
MspR9I CCNGG 3 cut(s) 169, 201, 755
MvaI CCWGG 1 cut(s) 755
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 2 cut(s) 259, 943
NciI CCSGG 2 cut(s) 169, 201
NdeII GATC 3 cut(s) 519, 585, 1129
NlaIII CATG 5 cut(s) 78, 651, 661, 748, 840
NlaIV GGNNCC 5 cut(s) 80, 198, 327, 456, 521
NmuCI GTSAC 1 cut(s) 554
NspI RCATGY 1 cut(s) 651
OliI CACNNNNGTG 1 cut(s) 552
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 2 cut(s) 617, 654
PkrI GCNGC 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 994
PshAI GACNNNNGTC 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 994
Psp6I CCWGG 1 cut(s) 753
PspFI CCCAGC 2 cut(s) 27, 253
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 5 cut(s) 80, 198, 327, 456, 521
PspPI GGNCC 5 cut(s) 166, 216, 442, 827, 994
PspPPI RGGWCCY 1 cut(s) 994
PstNI CAGNNNCTG 1 cut(s) 908
PsuI RGATCY 2 cut(s) 519, 1129
PvuII CAGCTG 2 cut(s) 31, 937
RsaI GTAC 3 cut(s) 142, 163, 687
RsaNI GTAC 3 cut(s) 141, 162, 686
RseI CAYNNNNRTG 1 cut(s) 552
SaqAI TTAA 3 cut(s) 612, 801, 920
SatI GCNGC 1 cut(s) 82
Sau3AI GATC 3 cut(s) 519, 585, 1129
Sau96I GGNCC 5 cut(s) 166, 216, 442, 827, 994
ScrFI CCNGG 3 cut(s) 169, 201, 755
SfaNI GCATC 2 cut(s) 571, 712
SinI GGWCC 1 cut(s) 994
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 2 cut(s) 611, 919
SmoI CTYRAG 2 cut(s) 611, 919
SpeI ACTAGT 1 cut(s) 229
Sse9I AATT 5 cut(s) 190, 264, 527, 639, 735
SsiI CCGC 5 cut(s) 82, 126, 332, 878, 931
SspI AATATT 1 cut(s) 1088
SspMI CTAG 3 cut(s) 230, 318, 942
StyD4I CCNGG 3 cut(s) 167, 199, 753
TaaI ACNGT 5 cut(s) 361, 424, 554, 904, 1012
TasI AATT 5 cut(s) 190, 264, 527, 639, 735
TatI WGTACW 1 cut(s) 140
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 2 cut(s) 617, 654
Tru1I TTAA 3 cut(s) 612, 801, 920
Tru9I TTAA 3 cut(s) 612, 801, 920
TscAI CASTG 2 cut(s) 136, 604
TseFI GTSAC 1 cut(s) 554
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 4 cut(s) 300, 407, 646, 1043
TspRI CASTG 2 cut(s) 136, 604
Vha464I CTTAAG 2 cut(s) 611, 919
VpaK11BI GGWCC 1 cut(s) 994
XapI RAATTY 2 cut(s) 190, 527
XceI RCATGY 1 cut(s) 651
XmiI GTMKAC 1 cut(s) 226
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 3 cut(s) 230, 318, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.