FvH4_2g30210

Enhancer of polycomb-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010036
Physical Location & Seq
Forward (+)
23360985 .. 23367642
6658 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30210.t1

Sequence Viewer

Length: 4359 bp
ATGGAAAATAGAGTAGAGATTTCACATGGCACTGAAATTCCCAGGAGATCGAGATCTTTGGATGTTAAGAGTTTGTATAGGTCCAGGAGTACAAAGGAGGCTGAGAATCAGAGTTTGAAGAGGAATGGGAGTGAGGGGGATGGGGATGGGGAGAAGAAGAAGAAGAGTAGGAAGGAGGTGTCCCTCAGTAGTCTCAAGAATGTTAATAGTAGTAGTAGTAGTAGCTGGAAGAATATAGATAAAGAGTATGATAGAGGGTTGGAGTCTGGTTCGCATGATCCGGAGGCATCGAATTCGGGGTCGAGTCAGAAATTGGATAGCGGTAGTAGGTTAAACAGCGTGTCACAGCTTAGTTTGGATAATAGTGGCATTCAAATCCCGAGGCGGAAGAGGGGATTTGTGGGGAGGAAAAAGTTTGAGGGTGGTCAGGCGTTGAAGCTGTCGGATGAGTCTGCCGGTAAAGCAAGTATTGCTGATCAGAATCATCAGGTAGCTAAGTTAAGTGGTGAGGAGTTGGATAGTCAGGCTGAGGGTTGGAAGGCTGAAAGGAACAAAGGTTTGGATGAATGCAAAGAAAATCTAAATAGTGAGTTGAATGGAGCTCTTCATGCAAAGAAAGAAAATGCTCTTGAGAGTCGGTCAGTCGTAAGCAATGGTAATTCATCTTTGAAAAAGTCCCGGAGGAAAAGTCGGAAAAGCAAGGACCTGTCATCTGATAGCAGAACTGATGCAAAGAAGGCTGAGCCTTTGGTAAATAGTTCTACTAAAGCTTGCCAGGCTTCACATGAAGACGAGGAGGAGAATCTTGAAGAGAATGCAGCAATGATGCTTTCATCAAGGTTTGATCCAAGCTGTACTGGGTTTTCATTGAATGCCAAGGCTTGTGCGATGCAATCTTCAAACGGATTGTCTGGTCAGGATTTTGATGGTCATATGTCGAAGTCATTGTCTGGATCCGAATCTCCGTCTATTGATAATGCCGGTAGAACACTTAGGCCCAGGCCTAGGAAGCATCACAAAGAAAAGAAAGGTACGAGGAAAAGACGCCACTTCTATGAAATCTTCTTTGGGGACTTGGATGCTTGTTGGGTAGTGAACCGTAGGATCAAGGTCTTCTGGCCTTTGGACCAAAGTTGGTACTATGGCCTTGTGAATGATTATGACAAGGACAAAAAACTTCACCATATTAGATACGATGACCGTGAAGAAGAATGGATTGATCTTCAACACGAGAGGTTCAAGCTCTTGCTACTTCCTACTGAAGTTCCTGGTAAGGCAAAGAAAAGATCATTCATACGGATTACAGGTTCTGAAGAGAGGGAAGAAAACTTGAAACCCAGAAAAGAAAAGAAAAAAAGAGACTTGATGTCAGAGGATGATAGCTGTATAGGCAGCTGTATGGATTCTGAACCTATTATTTCCTGGCTGGCGAGATCAACCCGTCGGATCAAGTCTCCTTCTCATGCTGTGAAGAAGCAAAAAACATCTGGTCTATCTCCAAAGTCCCTGCCAACCTTGTCTGACAGTGCTGGTACTCATGGGTGTTTGGGTGATGTTTCCTCTAGAAGGGATACAAGTAAATCATCTAGTAATTCTGGCAGATATTCTGATGCTTTGAGGGAAGAGAAGCGTGCTCCAGAGGGAGACATTTACCCGGAAGATAGCAGAATGCCTATTGTTTATTATCGGAAGCGATTGCGCAAGACTGGCTCTGTATTGTCTCAAATATACAAGGATGAGCATGCTTCCATGTATGGACATCGTTGCTGTACTTCTGTTACTCCTGTTGAGGAGATTTGGGACTTGGAAGAGCCAGATGATCATGTTGTTATATTGGATCGCAGTTGGCCATTGTGGTATAGTGATGGTGCAGGGTTGCTAAAGTTAACACTTCCTTGGGTAGAATCAGGAAAAGTTATATTTAAATGCCTTCAACTGCATTCACTAATTAATGATTCCCTTGGAGTAGAGTTGTTACGGTTCTGTCATGCTGCAATGCTGCTTCGGCATGGTATTGTGGTGATCACATGGCCAAAGATTCATCTGGAGATGCTTTTTGTTGATAATGTAGTTGGACTGAGGTTTCTTTTGTTTGAGGGTTGCTTGAAGCAGGCAGTAGTCTTGGTTTTTCTAATCCTGACATTATTTCATCAACCTAATGATCAAGGGAAGCTAACTGATTTCCAGTTACCAGCAACTTCAATCAGGTTCAAATTCTCTTGTGTTCAGCATCTTGGAAAGGAACTTGTGTTTGCATTCTATAACTTCTGTCGAGTTAAGAATTCAAAGTGGATGCACCTAGACAATAAACTCGGGAGGCATTGTTTACTCACCAAGAAACTGCCTCTTTCTGAATGCACCTATGATAATATCATGGCACTTCAAAATGGAATAAATCAGTCACCTTGTATCACTCTTTATGGGCAACCCTCCTCTGTCAAGGCTACACAGAAGAGGTCCAGGCAGGGGATTAACTTCATGGGAGGTTCCCGAGAAGTTGGTTTTGTAAATATCAGCCATTCTGCTACTCATTCTGATGAGATCCACAGAAAGCTTCCTCCATTTGCTCTATCTTTTACAGCTGCACCTACTTTCTTCATTAATTTGCATCTTAAGTTGCTTATGGAACACAGGGTTGCTAATATCTGCTTTCAAGACCGTGATTCAGAAATTACTCCTGAGAACAATTTGAAGGCTTCAGAAAATGTGGCTACCTCTGGTGGACCCTGTACTAAACTGGTAACAGAAGCATCTCTTTCTATTTGCAGTCACCGAGGTCGGATAAAGTCATCTCAGCTTTACCAAAATTGTGTTGTTAATGTTGCTGGAGCGTCTTCTAGGACTTCCGCTGGCAGAGATAAAGCTGATACTAGTTCCCGTTCTATTGTGAACGGGCTTACTGTTGAAATTCCACCTTTTGACCAATCTGAGAAGTTTGTGGAGAGGGAGATACAGAGTGCTGAACAGCCTACTGATTTTTCATTGAATATGAATGGTAGCATTATCCCCAGTCCCAGCCCCACTGCTCCCAGAAGCACAGGGCAACGAAATAGGAATAGCATGTCATCATTTGGAAACCTCTCACATTGTTGGTCAGATGGAAAGGCTGACATTTTCCATAATGGTTTTGGCAATGGACCGAAGAAACCCCGAACTCAAGTCTCATACACATTGCCTTGTGGAGGTTCTGATGGTAGCTCCAAGCAAAGAAACGTTCACAAAGGGCTTCCTAATAAACGAATTAGAAGGGCCAGTGAGAAGAGGTCGTTAGACACTTCCAGAGGTTCGCAAAGGAACTTGGAGCTGCTAACCTGCGAGGCAAATGTATTAATTACTGCAAGTGACAGAGGGTGGAGAGAAAATGGGGCACGTGTAGCATTAGAACAATTTGATAATAGTGAGTGGAAGCTTGCTGTTAAACTTTCAGGAACTACCAAGTATTTATACAAAGCTCATCAATTTTTGCAGCCTGGGTCAACAAACCGCTATACTCATGTTATGATGTGGAAAGGAGGAAAGGATTGGTTCTTGGAGTTTCCAGATAGAAGCCAGTGGGCCCTTTTCAAGGAGATGCATGAAGAGTGTTACAATCGGAACCTTCGGTCATCAGTTAAAAACATTCCTATTCCTGGGGTTCGCTTAGTAGAAGATATTGATGATAATGGAATAGAAATAGCATTTCTTCGCAGTTCTACCAAGTACTTCCAGCAAATGAAAACAGATGTTGAGATGGCTTTAGATCCATCTCGTATCTTGTATGACATGGATAGTGATGATGAGCGATGGATTTTGAAGTTTCGAAATTCTTCAGAAATGGACAAGAGTAGCTCAACAGAGATTGGCGAGGAGATGTTTGAGAAGACAATGGACATGTTTGAGAAGGCTGCATATGTTCAGCAATGTGATCAGTTCACATCTGAAGAAATAGAAGAGTTCATGACTGGTCTTGGGCCCATGGATTTGCTTAAAACCATATATGAGCATTGGCGTCAGAAACGGCTGAGAAAGGGAATGCCTTTAATCCGACATCTCCAGCCACCTTCATGGGAAATATATCAGAAACAAGTGAGGGAGTGGGAGCAAGTTATGACAAAGATGAATACCACACTTGCAAATGGAAGCCGTGAGAAGGCTGCACCAGTTGAGAAGCCACCGATGTATGCTTTTTGTCTGAAACCGCGGGGCCTGGAAGTTCCCAATAAGGGATCAAAGCAACGGTCACAGAAGAAATATTCTATTTCTGCACATACCAATGCGGTGTTAGGAGATCAGGATGGTTTCCATTCTATTGGAGATCAAGTGGGTTTGCATTCGGTGATGAAAAATTTGCATATTCAGGCCATAACTATGAATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPL1

Protein Analysis

1453

Amino Acids

164.13

Weight (kDa)

9.12

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPL1 PF10513 1200 - 1294 8.4e-09 Enhancer of polycomb-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1700
Acc36I ACCTGC 1 cut(s) 3320
AccII CGCG 1 cut(s) 4180
AccIII TCCGGA 1 cut(s) 280
AciI CCGC 7 cut(s) 321, 385, 2847, 3484, 4178, 4180, 4256
AclI AACGTT 1 cut(s) 3213
AcoI YGGCCR 2 cut(s) 1847, 2030
AcsI RAATTY 7 cut(s) 36, 292, 2213, 2281, 2907, 3802, 4324
AcuI CTGAAG 5 cut(s) 1281, 1332, 2682, 3792, 3939
AcvI CACGTG 1 cut(s) 3371
AcyI GRCGYC 2 cut(s) 1045, 3988
AfaI GTAC 8 cut(s) 91, 856, 1033, 1139, 1534, 1771, 2731, 3701
AfiI CCNNNNNNNGG 8 cut(s) 1005, 1566, 2466, 3182, 3565, 3629, 4129, 4202
AflII CTTAAG 1 cut(s) 2612
AflIII ACRYGT 2 cut(s) 3370, 3870
AhdI GACNNNNNGTC 1 cut(s) 1366
AhlI ACTAGT 1 cut(s) 2870
AjuI GAANNNNNNNTTGG 4 cut(s) 542, 574, 1050, 1082
AloI GAACNNNNNNTCC 2 cut(s) 1291, 1323
Alw21I GWGCWC 2 cut(s) 604, 1636
Alw26I GTCTC 6 cut(s) 197, 1353, 1458, 1638, 1725, 3166
AlwNI CAGNNNCTG 1 cut(s) 1310
Ama87I CYCGRG 3 cut(s) 379, 2312, 2490
Aor13HI TCCGGA 1 cut(s) 280
ApaI GGGCCC 2 cut(s) 3559, 3954
ApeKI GCWGC 9 cut(s) 818, 1392, 1991, 1999, 2582, 3304, 3466, 3884, 4133
ApoI RAATTY 7 cut(s) 36, 292, 2213, 2281, 2907, 3802, 4324
ArsI GACNNNNNNTTYG 8 cut(s) 284, 316, 893, 925, 932, 964, 4202, 4234
AseI ATTAAT 3 cut(s) 1950, 2601, 3329
Asp700I GAANNNNTTC 1 cut(s) 3805
AspA2I CCTAGG 1 cut(s) 1004
AspLEI GCGC 1 cut(s) 1701
AsuC2I CCSGG 2 cut(s) 679, 1655
AsuHPI GGTGA 8 cut(s) 518, 1172, 1562, 2032, 2323, 2394, 2762, 4327
AsuII TTCGAA 1 cut(s) 3799
AvaI CYCGRG 3 cut(s) 379, 2312, 2490
AvaII GGWCC 6 cut(s) 81, 703, 1126, 2457, 2723, 3137
AvrII CCTAGG 1 cut(s) 1004
BaeGI GKGCMC 3 cut(s) 3370, 3559, 3954
BalI TGGCCA 2 cut(s) 1849, 2032
BamHI GGATCC 1 cut(s) 953
BanII GRGCYC 3 cut(s) 604, 3559, 3954
BauI CACGAG 1 cut(s) 1229
BbrPI CACGTG 1 cut(s) 3371
BbsI GAAGAC 4 cut(s) 795, 1105, 2826, 3866
Bbv12I GWGCWC 2 cut(s) 604, 1636
BbvCI CCTCAGC 1 cut(s) 528
BbvI GCAGC 9 cut(s) 830, 1404, 1978, 1986, 2569, 3291, 3478, 3871, 4120
BceAI ACGGC 2 cut(s) 4013, 4107
BcgI CGANNNNNNTGC 2 cut(s) 276, 310
BciVI GTATCC 1 cut(s) 1564
BclI TGATCA 5 cut(s) 475, 1819, 2022, 2161, 3904
BcnI CCSGG 2 cut(s) 679, 1655
BcoDI GTCTC 6 cut(s) 197, 1353, 1458, 1638, 1725, 3166
BcuI ACTAGT 1 cut(s) 2870
BfaI CTAG 6 cut(s) 1005, 1563, 1587, 2300, 2838, 2871
BfrI CTTAAG 1 cut(s) 2612
BfuAI ACCTGC 1 cut(s) 3320
BfuI GTATCC 1 cut(s) 1564
BglII AGATCT 1 cut(s) 53
BisI GCNGC 9 cut(s) 819, 1393, 1992, 2000, 2583, 3305, 3467, 3885, 4134
BlnI CCTAGG 1 cut(s) 1004
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 9 cut(s) 820, 1394, 1993, 2001, 2584, 3306, 3468, 3886, 4135
BmcAI AGTACT 1 cut(s) 3701
Bme18I GGWCC 6 cut(s) 81, 703, 1126, 2457, 2723, 3137
BmeRI GACNNNNNGTC 1 cut(s) 1366
BmeT110I CYCGRG 3 cut(s) 379, 2312, 2490
BmiI GGNNCC 7 cut(s) 955, 2488, 2725, 3557, 3596, 3952, 4184
BmrI ACTGGG 2 cut(s) 867, 3003
BmuI ACTGGG 2 cut(s) 867, 3003
BpiI GAAGAC 4 cut(s) 795, 1105, 2826, 3866
BpmI CTGGAG 4 cut(s) 1620, 2066, 2847, 4016
Bpu10I CCTNAGC 1 cut(s) 528
Bpu1102I GCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 1 cut(s) 3799
BpuEI CTTGAG 3 cut(s) 179, 650, 3141
BpuMI CCSGG 2 cut(s) 679, 1655
BsaAI YACGTR 1 cut(s) 3371
BsaBI GATNNNNATC 3 cut(s) 52, 480, 958
BsaHI GRCGYC 2 cut(s) 1045, 3988
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 6 cut(s) 673, 703, 2308, 2338, 4284, 4314
Bsc4I CCNNNNNNNGG 8 cut(s) 1005, 1566, 2466, 3182, 3565, 3629, 4129, 4202
Bse118I RCCGGY 2 cut(s) 455, 980
Bse1I ACTGG 9 cut(s) 862, 1711, 2185, 2742, 3009, 3252, 3550, 3946, 4139
Bse3DI GCAATG 6 cut(s) 658, 828, 2001, 3139, 3170, 3905
Bse8I GATNNNNATC 3 cut(s) 52, 480, 958
BseAI TCCGGA 1 cut(s) 280
BseJI GATNNNNATC 3 cut(s) 52, 480, 958
BseLI CCNNNNNNNGG 8 cut(s) 1005, 1566, 2466, 3182, 3565, 3629, 4129, 4202
BseMI GCAATG 6 cut(s) 658, 828, 2001, 3139, 3170, 3905
BseMII CTCAG 9 cut(s) 93, 199, 519, 732, 2069, 2670, 2807, 2919, 3992
BseNI ACTGG 9 cut(s) 862, 1711, 2185, 2742, 3009, 3252, 3550, 3946, 4139
BseRI GAGGAG 6 cut(s) 524, 809, 812, 1805, 2422, 3860
BseSI GKGCMC 3 cut(s) 3370, 3559, 3954
BseXI GCAGC 9 cut(s) 830, 1404, 1978, 1986, 2569, 3291, 3478, 3871, 4120
BseYI CCCAGC 1 cut(s) 3014
BsgI GTGCAG 4 cut(s) 1890, 2568, 4119, 4227
Bsh1236I CGCG 1 cut(s) 4180
BsiHKAI GWGCWC 2 cut(s) 604, 1636
BsiHKCI CYCGRG 3 cut(s) 379, 2312, 2490
BsiSI CCGG 5 cut(s) 281, 456, 679, 981, 1655
BslFI GGGAC 6 cut(s) 166, 661, 1085, 1489, 1814, 2997
BslI CCNNNNNNNGG 8 cut(s) 1005, 1566, 2466, 3182, 3565, 3629, 4129, 4202
BsmAI GTCTC 6 cut(s) 197, 1353, 1458, 1638, 1725, 3166
BsmFI GGGAC 6 cut(s) 166, 661, 1085, 1489, 1814, 2997
BsoBI CYCGRG 3 cut(s) 379, 2312, 2490
Bsp119I TTCGAA 1 cut(s) 3799
Bsp120I GGGCCC 2 cut(s) 3555, 3950
Bsp1286I GDGCHC 5 cut(s) 604, 1636, 3370, 3559, 3954
Bsp13I TCCGGA 1 cut(s) 280
Bsp1720I GCTNAGC 1 cut(s) 741
Bsp19I CCATGG 1 cut(s) 3954
BspACI CCGC 7 cut(s) 321, 385, 2847, 3484, 4178, 4180, 4256
BspCNI CTCAG 9 cut(s) 94, 198, 520, 733, 2070, 2671, 2806, 2920, 3993
BspEI TCCGGA 1 cut(s) 280
BspFNI CGCG 1 cut(s) 4180
BspHI TCATGA 1 cut(s) 3936
BspLI GGNNCC 7 cut(s) 955, 2488, 2725, 3557, 3596, 3952, 4184
BspMI ACCTGC 1 cut(s) 3320
BspQI GCTCTTC 2 cut(s) 609, 1803
BspT104I TTCGAA 1 cut(s) 3799
BspTI CTTAAG 1 cut(s) 2612
BsrDI GCAATG 6 cut(s) 658, 828, 2001, 3139, 3170, 3905
BsrFI RCCGGY 2 cut(s) 455, 980
BsrI ACTGG 9 cut(s) 862, 1711, 2185, 2742, 3009, 3252, 3550, 3946, 4139
BssAI RCCGGY 2 cut(s) 455, 980
BssNI GRCGYC 2 cut(s) 1045, 3988
BssSI CACGAG 1 cut(s) 1229
BssT1I CCWWGG 5 cut(s) 876, 1004, 1895, 1960, 3954
Bst2BI CACGAG 1 cut(s) 1229
Bst4CI ACNGT 7 cut(s) 1100, 1202, 1526, 1980, 2660, 2902, 4218
BstACI GRCGYC 2 cut(s) 1045, 3988
BstAFI CTTAAG 1 cut(s) 2612
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 3371
BstBI TTCGAA 1 cut(s) 3799
BstC8I GCNNGC 7 cut(s) 772, 1428, 1632, 1743, 2112, 2851, 3411
BstDSI CCRYGG 2 cut(s) 3954, 4178
BstENI CCTNNNNNAGG 3 cut(s) 1564, 3180, 3563
BstFNI CGCG 1 cut(s) 4180
BstHHI GCGC 1 cut(s) 1701
BstMAI GTCTC 6 cut(s) 197, 1353, 1458, 1638, 1725, 3166
BstNSI RCATGY 3 cut(s) 1745, 3064, 3874
BstSLI GKGCMC 3 cut(s) 3370, 3559, 3954
BstUI CGCG 1 cut(s) 4180
BstV1I GCAGC 9 cut(s) 830, 1404, 1978, 1986, 2569, 3291, 3478, 3871, 4120
BstV2I GAAGAC 4 cut(s) 795, 1105, 2826, 3866
BstX2I RGATCY 4 cut(s) 53, 953, 2541, 3739
BstXI CCANNNNNNTGG 2 cut(s) 4044, 4289
BstYI RGATCY 4 cut(s) 53, 953, 2541, 3739
BsuI GTATCC 1 cut(s) 1564
BtgI CCRYGG 2 cut(s) 3954, 4178
BtgZI GCGATG 2 cut(s) 902, 3796
BtsI GCAGTG 1 cut(s) 3021
BtsIMutI CAGTG 5 cut(s) 30, 1531, 3021, 3259, 3557
BveI ACCTGC 1 cut(s) 3320
Cac8I GCNNGC 7 cut(s) 772, 1428, 1632, 1743, 2112, 2851, 3411
CaiI CAGNNNCTG 1 cut(s) 1310
CciI TCATGA 1 cut(s) 3936
CfoI GCGC 1 cut(s) 1701
Cfr10I RCCGGY 2 cut(s) 455, 980
Cfr42I CCGCGG 1 cut(s) 4181
CseI GACGC 3 cut(s) 1053, 2820, 3977
Csp6I GTAC 8 cut(s) 90, 855, 1032, 1138, 1533, 1770, 2730, 3700
CviQI GTAC 8 cut(s) 90, 855, 1032, 1138, 1533, 1770, 2730, 3700
DraI TTTAAA 1 cut(s) 1924
DriI GACNNNNNGTC 1 cut(s) 1366
EaeI YGGCCR 2 cut(s) 1847, 2030
Eam1105I GACNNNNNGTC 1 cut(s) 1366
EciI GGCGGA 1 cut(s) 400
Ecl136II GAGCTC 1 cut(s) 602
Eco130I CCWWGG 5 cut(s) 876, 1004, 1895, 1960, 3954
Eco147I AGGCCT 1 cut(s) 1003
Eco24I GRGCYC 3 cut(s) 604, 3559, 3954
Eco47I GGWCC 6 cut(s) 81, 703, 1126, 2457, 2723, 3137
Eco53kI GAGCTC 1 cut(s) 602
Eco57I CTGAAG 5 cut(s) 1281, 1332, 2682, 3792, 3939
Eco72I CACGTG 1 cut(s) 3371
Eco88I CYCGRG 3 cut(s) 379, 2312, 2490
EcoICRI GAGCTC 1 cut(s) 602
EcoNI CCTNNNNNAGG 3 cut(s) 1564, 3180, 3563
EcoO109I RGGNCCY 3 cut(s) 703, 3556, 4183
EcoRI GAATTC 2 cut(s) 292, 2281
EcoT14I CCWWGG 5 cut(s) 876, 1004, 1895, 1960, 3954
EcoT22I ATGCAT 1 cut(s) 3576
EcoT38I GRGCYC 3 cut(s) 604, 3559, 3954
ErhI CCWWGG 5 cut(s) 876, 1004, 1895, 1960, 3954
FalI AAGNNNNNCTT 2 cut(s) 2739, 2771
FaqI GGGAC 6 cut(s) 166, 661, 1085, 1489, 1814, 2997
FauI CCCGC 1 cut(s) 4173
FauNDI CATATG 2 cut(s) 933, 3889
FbaI TGATCA 5 cut(s) 475, 1819, 2022, 2161, 3904
Fnu4HI GCNGC 9 cut(s) 819, 1393, 1992, 2000, 2583, 3305, 3467, 3885, 4134
FriOI GRGCYC 3 cut(s) 604, 3559, 3954
Fsp4HI GCNGC 9 cut(s) 819, 1393, 1992, 2000, 2583, 3305, 3467, 3885, 4134
FspBI CTAG 6 cut(s) 1005, 1563, 1587, 2300, 2838, 2871
FspI TGCGCA 1 cut(s) 1700
GlaI GCGC 1 cut(s) 1700
GluI GCNGC 9 cut(s) 819, 1393, 1992, 2000, 2583, 3305, 3467, 3885, 4134
GsaI CCCAGC 1 cut(s) 3018
GsuI CTGGAG 4 cut(s) 1620, 2066, 2847, 4016
HapII CCGG 5 cut(s) 281, 456, 679, 981, 1655
HgaI GACGC 3 cut(s) 1053, 2820, 3977
HhaI GCGC 1 cut(s) 1701
Hin1I GRCGYC 2 cut(s) 1045, 3988
Hin6I GCGC 1 cut(s) 1699
HinP1I GCGC 1 cut(s) 1699
HincII GTYRAC 2 cut(s) 1887, 3477
HindII GTYRAC 2 cut(s) 1887, 3477
HindIII AAGCTT 3 cut(s) 768, 2552, 3407
HpaI GTTAAC 1 cut(s) 1887
HpaII CCGG 5 cut(s) 281, 456, 679, 981, 1655
HphI GGTGA 8 cut(s) 518, 1172, 1562, 2032, 2323, 2394, 2762, 4327
Hpy166II GTNNAC 8 cut(s) 1096, 1887, 2327, 2723, 2890, 3217, 3477, 3912
Hpy8I GTNNAC 8 cut(s) 1096, 1887, 2327, 2723, 2890, 3217, 3477, 3912
Hpy99I CGWCG 1 cut(s) 1446
HpyCH4III ACNGT 7 cut(s) 1100, 1202, 1526, 1980, 2660, 2902, 4218
HpyCH4IV ACGT 2 cut(s) 3213, 3370
HpySE526I ACGT 2 cut(s) 3213, 3370
Hsp92I GRCGYC 2 cut(s) 1045, 3988
HspAI GCGC 1 cut(s) 1699
Kpn2I TCCGGA 1 cut(s) 280
Ksp22I TGATCA 5 cut(s) 475, 1819, 2022, 2161, 3904
KspAI GTTAAC 1 cut(s) 1887
KspI CCGCGG 1 cut(s) 4181
LguI GCTCTTC 2 cut(s) 609, 1803
LmnI GCTCC 7 cut(s) 599, 1639, 2828, 3031, 3203, 3301, 4078
Lsp1109I GCAGC 9 cut(s) 830, 1404, 1978, 1986, 2569, 3291, 3478, 3871, 4120
MaeI CTAG 6 cut(s) 1005, 1563, 1587, 2300, 2838, 2871
MaeII ACGT 2 cut(s) 3213, 3370
MflI RGATCY 4 cut(s) 53, 953, 2541, 3739
MhlI GDGCHC 5 cut(s) 604, 1636, 3370, 3559, 3954
MlsI TGGCCA 2 cut(s) 1849, 2032
MluNI TGGCCA 2 cut(s) 1849, 2032
MlyI GAGTC 4 cut(s) 272, 313, 458, 643
MmeI TCCRAC 9 cut(s) 240, 423, 495, 515, 671, 1424, 2053, 2759, 4048
Mox20I TGGCCA 2 cut(s) 1849, 2032
Mph1103I ATGCAT 1 cut(s) 3576
MroI TCCGGA 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 3805
MscI TGGCCA 2 cut(s) 1849, 2032
MslI CAYNNNNRTG 5 cut(s) 1053, 2535, 4042, 4251, 4346
Msp20I TGGCCA 2 cut(s) 1849, 2032
MspA1I CMGCKG 4 cut(s) 1395, 2582, 2849, 4180
MspCI CTTAAG 1 cut(s) 2612
MspI CCGG 5 cut(s) 281, 456, 679, 981, 1655
MvnI CGCG 1 cut(s) 4180
NciI CCSGG 2 cut(s) 679, 1655
NcoI CCATGG 1 cut(s) 3954
NdeI CATATG 2 cut(s) 933, 3889
NlaIV GGNNCC 7 cut(s) 955, 2488, 2725, 3557, 3596, 3952, 4184
NmuCI GTSAC 5 cut(s) 342, 2400, 2768, 3341, 4218
NsbI TGCGCA 1 cut(s) 1700
NsiI ATGCAT 1 cut(s) 3576
NspI RCATGY 3 cut(s) 1745, 3064, 3874
NspV TTCGAA 1 cut(s) 3799
PaeI GCATGC 1 cut(s) 1745
PagI TCATGA 1 cut(s) 3936
PceI AGGCCT 1 cut(s) 1003
PciI ACATGT 1 cut(s) 3870
PciSI GCTCTTC 2 cut(s) 609, 1803
PdmI GAANNNNTTC 1 cut(s) 3805
PfoI TCCNGGA 2 cut(s) 83, 677
PkrI GCNGC 9 cut(s) 820, 1394, 1993, 2001, 2584, 3306, 3468, 3886, 4135
PleI GAGTC 4 cut(s) 271, 312, 457, 642
PmaCI CACGTG 1 cut(s) 3371
PmlI CACGTG 1 cut(s) 3371
PpsI GAGTC 4 cut(s) 271, 312, 457, 642
Ppu21I YACGTR 1 cut(s) 3371
PpuMI RGGWCCY 1 cut(s) 703
PscI ACATGT 1 cut(s) 3870
PshBI ATTAAT 3 cut(s) 1950, 2601, 3329
Psp124BI GAGCTC 1 cut(s) 604
Psp1406I AACGTT 1 cut(s) 3213
Psp5II RGGWCCY 1 cut(s) 703
PspCI CACGTG 1 cut(s) 3371
PspFI CCCAGC 1 cut(s) 3014
PspN4I GGNNCC 7 cut(s) 955, 2488, 2725, 3557, 3596, 3952, 4184
PspOMI GGGCCC 2 cut(s) 3555, 3950
PspPPI RGGWCCY 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 1310
PsuI RGATCY 4 cut(s) 53, 953, 2541, 3739
PvuII CAGCTG 2 cut(s) 1395, 2582
RsaI GTAC 8 cut(s) 91, 856, 1033, 1139, 1534, 1771, 2731, 3701
RsaNI GTAC 8 cut(s) 90, 855, 1032, 1138, 1533, 1770, 2730, 3700
RseI CAYNNNNRTG 5 cut(s) 1053, 2535, 4042, 4251, 4346
SacI GAGCTC 1 cut(s) 604
SacII CCGCGG 1 cut(s) 4181
SapI GCTCTTC 2 cut(s) 609, 1803
SatI GCNGC 9 cut(s) 819, 1393, 1992, 2000, 2583, 3305, 3467, 3885, 4134
ScaI AGTACT 1 cut(s) 3701
SchI GAGTC 4 cut(s) 272, 313, 458, 643
SduI GDGCHC 5 cut(s) 604, 1636, 3370, 3559, 3954
Sfr303I CCGCGG 1 cut(s) 4181
SfuI TTCGAA 1 cut(s) 3799
SgrBI CCGCGG 1 cut(s) 4181
SinI GGWCC 6 cut(s) 81, 703, 1126, 2457, 2723, 3137
SmiI ATTTAAAT 1 cut(s) 1924
SmiMI CAYNNNNRTG 5 cut(s) 1053, 2535, 4042, 4251, 4346
SmlI CTYRAG 4 cut(s) 194, 629, 2612, 3156
SmoI CTYRAG 4 cut(s) 194, 629, 2612, 3156
SpeI ACTAGT 1 cut(s) 2870
SphI GCATGC 1 cut(s) 1745
SseBI AGGCCT 1 cut(s) 1003
SsiI CCGC 7 cut(s) 321, 385, 2847, 3484, 4178, 4180, 4256
SspI AATATT 1 cut(s) 4232
SspMI CTAG 6 cut(s) 1005, 1563, 1587, 2300, 2838, 2871
SstI GAGCTC 1 cut(s) 604
StuI AGGCCT 1 cut(s) 1003
StyI CCWWGG 5 cut(s) 876, 1004, 1895, 1960, 3954
SwaI ATTTAAAT 1 cut(s) 1924
TaaI ACNGT 7 cut(s) 1100, 1202, 1526, 1980, 2660, 2902, 4218
TaiI ACGT 2 cut(s) 3216, 3373
TaqI TCGA 6 cut(s) 50, 290, 302, 938, 2272, 3799
TaqII GACCGA 3 cut(s) 627, 3154, 3591
TatI WGTACW 5 cut(s) 89, 854, 1769, 2729, 3699
TscAI CASTG 5 cut(s) 37, 1531, 3028, 3259, 3557
TseFI GTSAC 5 cut(s) 342, 2400, 2768, 3341, 4218
TseI GCWGC 9 cut(s) 818, 1392, 1991, 1999, 2582, 3304, 3466, 3884, 4133
Tsp45I GTSAC 5 cut(s) 342, 2400, 2768, 3341, 4218
TspGWI ACGGA 3 cut(s) 918, 954, 1312
TspRI CASTG 5 cut(s) 37, 1531, 3028, 3259, 3557
Vha464I CTTAAG 1 cut(s) 2612
VpaK11BI GGWCC 6 cut(s) 81, 703, 1126, 2457, 2723, 3137
VspI ATTAAT 3 cut(s) 1950, 2601, 3329
XagI CCTNNNNNAGG 3 cut(s) 1564, 3180, 3563
XapI RAATTY 7 cut(s) 36, 292, 2213, 2281, 2907, 3802, 4324
XbaI TCTAGA 1 cut(s) 1562
XceI RCATGY 3 cut(s) 1745, 3064, 3874
XcmI CCANNNNNNNNNTGG 1 cut(s) 3125
XmaJI CCTAGG 1 cut(s) 1004
XmnI GAANNNNTTC 1 cut(s) 3805
XspI CTAG 6 cut(s) 1005, 1563, 1587, 2300, 2838, 2871
ZrmI AGTACT 1 cut(s) 3701
Zsp2I ATGCAT 1 cut(s) 3576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.